Protocols: Cells were seeded at 400,000 cells per 60 mm dish and were allowed to grow for 48 hours.  Cell lines were cultured in a humidified incubator with 5% CO2 at 37C as recommended by ATCC (10% FBS, RPMI) supplemented with 100 units penicillin, 0.10 mg streptomycin and 0.25 g amphotericin B per ml.  Total RNA was isolated using the RNeasy kit according to manufacturerâ€™s instructions. 1 ug total RNA was used to generate stranded, poly-A selected NGS libraries using the NEBNext Ultra Directional RNA kit
isolate
not applicable
organism
Homo sapiens
sex
female
age
61
developmental stage
adult
disease
pancreatic adenocarcinoma
organism part
pancreas
cell_line
BxPC-3
genotype
gemcitabine resistant subclone 2
phenotype
gemcitabine resistant
ENA-FIRST-PUBLIC
2021-12-22
ENA-LAST-UPDATE
2021-12-22