Protocols: Sample for bulk RNA was collected by filtering 10 ml of culture in exponential phase (density of cells ≈ 10 mil) on 5 µm polycarbonate filters (Whatman).Filters were placed in cryoprotectant tubes with beads (Zirconium oxide beads, Precellys), amended with 600 µl of RLT buffer (Qiagen) which contained 1% of 2-Mercaptoethanol, shock frozen in liquid nitrogen and stored at -80 °C until further processing. Prior to RNA extraction, sample was taken out of -80, thawed on ice and subjected to bead beating (1x 6000 rmp, Precellys Evolution homogenizer)for 2 min. RNA from samples was further extracted with the RNeasy Qiagen kit (QIAGEN, Inc., Valencia, CA) following the kit instructions. RNA was re-eluted in 30 µl of nuclease free water. Input: RNA Type: Illumina TruSeq Stranded mRNA Option: Poly-A selection Category: default
ENA-FIRST-PUBLIC
2022-10-27
ENA-LAST-UPDATE
2022-10-27
External Id
SAMEA111452526
INSDC center alias
Department of Gene Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health,SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden
INSDC center name
Department of Gene Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health,SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden
INSDC first public
2022-10-27T14:00:03Z
INSDC last update
2022-10-27T14:00:03Z
INSDC status
public
Submitter Id
E-MTAB-12262:Heterocapsa RNA
broker name
ArrayExpress
sample name
E-MTAB-12262:Heterocapsa RNA
strain
dinoflagellate Heterocapsa sp. CCAP 1125/4