Protocols: Nuclei was isolated from frozen livers from young C57BL/6 mice (3 month old) using the protocol described in Krishnaswami et al. 2016 (Nature Protocols, 11: 499-524). Nuclei was purified using Iodixanol gradient (Optiprep, D1556, Sigma Aldrich Chemie). Nuclei were stained with Hoechst and FACS sorted according to their ploidy level diploid (2n) and tetraploid (4n) in 384-well plates.
ENA first public
2021-05-07
ENA last update
2021-03-11
External Id
SAMEA8230056
INSDC center alias
Helmholtz Pioneer Campus
INSDC center name
Helmholtz Pioneer Campus
INSDC first public
2021-05-07T00:27:00Z
INSDC last update
2021-03-11T09:48:15Z
INSDC status
public
Submitter Id
E-MTAB-93331615455990:MUC25641
age
3
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
individual
-
organism part
liver
ploidy
2n
post analysis well quality
pass
sample name
E-MTAB-93331615455990:MUC25641
sex
male
strain
C57Bl6/J