Protocols: Nuclei was isolated from frozen livers from young C57BL/6 mice (3 month old) using the protocol described in Krishnaswami et al. 2016 (Nature Protocols, 11: 499-524). Nuclei was purified using Iodixanol gradient (Optiprep, D1556, Sigma Aldrich Chemie). Nuclei were stained with Hoechst and FACS sorted according to their ploidy level diploid (2n) and tetraploid (4n) in 384-well plates.
ENA-FIRST-PUBLIC
2021-05-07T00:27:00Z
ENA-LAST-UPDATE
2021-03-11T09:48:18Z
External Id
SAMEA8230225
INSDC center name
Helmholtz Pioneer Campus
INSDC first public
2021-05-07T00:27:00Z
INSDC last update
2021-03-11T09:48:18Z
INSDC status
public
Submitter Id
E-MTAB-93331615455990:MUC25809
age
3
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
individual
-
organism part
liver
ploidy
2n
post analysis well quality
fail
sample name
E-MTAB-93331615455990:MUC25809
scientific_name
Mus musculus
sex
male
strain
C57-CCl4 treated