The brain was dissected and stored at 80 C until the RNA isolation.Total RNA was isolated from the brain tissue samples using the MagNA Pure Compact RNA Isolation Kit (Roche, Basel, Switzerland) according to the manufacturers protocol. Double stranded cDNA library was prepared by using an Illumina TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturers guidelines from 0,3 g of total RNA.The cDNA libraries were normalized to 4 nM, pooled together in equal volumes, and sequenced with 75 bp single-end reads on the NextSeq 500 System (Illumina, USA).
strain
Zanzibar TAN 14-02
breed
The fish were kept at 27 C under 14:10 h light:dark regime in commercial Aquatic housing system (Aquaneering, Inc., San Diego, CA, USA). The feeding of fish larvae was carried out 3 times per day with newly hatched brine shrimp Artemia salina. At the age of sexual maturity, the fish was switched to diet containing meclofenoxate at 100 mg/kg in 2/2 week cycles of treatment.
age
12 months
sex
male
tissue
brain
sample_type
whole brain tissue
treatment
meclofenoxate at 100 mg/kg in 2/2 week cycles of treatment
replicate
3
BioSampleModel
Model organism or animal