Protocols: The fish were unsystematically divided into three groups of 270 fish with triplicates. The corresponding fish dietary treatments were prepared using a basic feed (purchased from Tongwei Group, Chengdu, China) for the control group (CG) and the same but supplemented with enrofloxacin (EG) or florfenicol (FG) at a concentration of 10 mg/kg.The midgut tissues of 12 individual fish from each of the three treatment groups were snap-frozen and aseptically harvested in liquid nitrogen for the transcriptomic analyses. Total RNA was extracted from the midgut of grass carp by using the TRlzol Reagent and following the manufacturer's instructions (Life Technologies, California, USA). cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.
ENA-FIRST-PUBLIC
2022-08-08
ENA-LAST-UPDATE
2022-08-08
External Id
SAMEA110472432
INSDC center alias
Zhongkai University of Agriculture and Engineering
INSDC center name
Zhongkai University of Agriculture and Engineering
INSDC first public
2022-08-08T16:39:05Z
INSDC last update
2022-08-08T16:39:05Z
INSDC status
public
Submitter Id
E-MTAB-12058:FG3
age
0.5
broker name
ArrayExpress
common name
grass carp
organism part
gut
sample name
E-MTAB-12058:FG3
sex
male
stimulus
florfenicol