Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 in an RNAse-inhibitor supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).
ENA first public
2021-12-06
ENA last update
2021-12-06
External Id
SAMEA10112543
INSDC center alias
UNIVERSITY OF CAMBRIDGE
INSDC center name
UNIVERSITY OF CAMBRIDGE
INSDC first public
2021-12-06T00:11:46Z
INSDC last update
2021-12-06T00:11:46Z
INSDC status
public
Submitter Id
E-MTAB-93491631874715:SLX.19811.i703_i505.HKJ5LBBXY
broker name
ArrayExpress
cell line
New2f
cell type
naïve pluripotent stem cell
common name
white-tufted-ear marmoset
developmental stage
embryo stage
organism part
blastocyst
post analysis well quality
pass
sample name
E-MTAB-93491631874715:SLX.19811.i703_i505.HKJ5LBBXY
single cell well quality
OK