Protocols: Cells were collected in exponential phase of the growth concentrated by centrifugation at 8000g for 5 min. Libraries were prepared with Magnatrix 8000+ (Nordiag), an eight-channel robotic workstation capable of running custom made scripts for in-tip magnetic bead separations41. Released mRNA-cDNA hybrids were transferred to the robotic workstation where they underwent second strand synthesis, end repair and in vitro transcription. Following transcription, sequencing adaptors are ligated to the RNA, and another round of cDNA synthesis is performed. Each step in the process is followed by a reaction clean-up step.
ENA-FIRST-PUBLIC
2022-10-27
ENA-LAST-UPDATE
2022-10-27
External Id
SAMEA111452601
INSDC center alias
Department of Gene Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health,SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden
INSDC center name
Department of Gene Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health,SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden
INSDC first public
2022-10-27T14:00:27Z
INSDC last update
2022-10-27T14:00:27Z
INSDC status
public
Submitter Id
E-MTAB-12261:Tet2
broker name
ArrayExpress
sample name
E-MTAB-12261:Tet2
spots with single cell detected
194
strain
630/1M