ATAC-seq MOLM1
Protocols: 100.000 cells (MUTZ3, MOLM1) were lysed in lysis buffer The MUTZ3 cell lines were cultured in αMEM (HyClone) with 20% fetal calf serum (FCS) and 20% conditioned 5637 medium. The 293T were cultured in DMEM (Gibco) with 10% FCS. All cell lines were supplemented with 50 U/mL penicillin and 50 μg/mL streptomycin. Viable frozen AML cells were thawed and resuspended in IMDM medium supplemented with: 20% BIT medium (Stem cell technologies), 1x β-mercaptoethanol (1000x Life technologies), 6 µg/ml LDL (Sigma Aldrich), human IL6, IL3, G-CSF, GM-CSF at 20 ng/ml and FLT3, SCF at 50 ng/ml (Peprotech). Immediately after lysis, nuclei were spun at 500g for 8 minutes at 4°C. After pelleting the nuclei, we the nuclei were resuspended in Tn5 transposase reaction mix (25 ul 2X TD buffer, 2.5 ul Tn5 transposase, and 22.5 ul nucleasefree water). The transposition reaction was performed at 37°C for 30 minutes. Immediately after the transposition reaction, DNA was purified using a Qiagen MinElute kit. The transposed library was PCR amplified using Nextera primers. The PCR reaction was subsequently cleaned up using AMPureXP beads.
ENA first public
2021-07-26
ENA last update
2021-07-26
External Id
SAMEA7745360
INSDC center alias
Department of Hematology, Erasmus University Medical Center
INSDC center name
Department of Hematology, Erasmus University Medical Center
INSDC first public
2021-07-26T12:17:16Z
INSDC last update
2021-07-26T12:17:16Z
INSDC status
public
Submitter Id
E-MTAB-9949:ATAC-seq MOLM1
broker name
ArrayExpress
cell line
MOLM-1
cell type
myeloblast
common name
human
developmental stage
adult
disease
acute myeloid leukemia
genotype
inv(3)
organism part
bone marrow
sample name
E-MTAB-9949:ATAC-seq MOLM1