Per biological replicate, cells were transduced at 750-1000X representation with the Digenic Paralog CRISPR Library in cell lines stably expressing S. Pyogenese Cas9. Cells were selected with puromycin for 3 days post-transduction and passaged for 16 days after selection. Genomic DNA was collected from these cells was PCR amplified and sequenced.
isolate
lentiviral integrate
age
58
biomaterial_provider
Genetic Perturbation Platform
sex
female
tissue
skin
cell_line
MELJUSO
disease
Skin Cancer
treatment
DMSO
DeplexedBarcode
CCAGTGAT
BioSampleModel
Human