do18191_3
Protocols: Nuclei were isolated from frozen livers from young C57BL/6 mice (3 month old) using the protocol described in Krishnaswami et al. 2016 (Nature Protocols, 11: 499-524). Nuclei were purified using Iodixanol gradient (Optiprep, D1556, Sigma Aldrich Chemie). Nuclei were stained with Hoechst and FACS sorted according to their ploidy level into tubes containing PBS/BSA 0,05%. The 4n nuclei samples were loaded in a Chromium Single Cell A Chip (10X Genomics, PN-120236) with the addition of the snRNAseq2 extra lysis reagents (NP-40 and triton-X) and were processed through the Chromium Single Cell Platform using the Chromium Single Cell 3′ Library and Gel Bead Kit v2 (10X Genomics, PN-120237) as per manufacturers instructions. After cDNA amplification, 25% of the cDNA was processed for Library preparation (10X Genomics, PN-120237) as per the manufacturer's instructions. The final libraries were quantified and pooled for sequencing.
ENA first public
2021-05-07
ENA last update
2021-03-17
External Id
SAMEA8268104
INSDC center alias
Helmholtz Pioneer Campus
INSDC center name
Helmholtz Pioneer Campus
INSDC first public
2021-05-07T00:27:00Z
INSDC last update
2021-03-17T08:13:19Z
INSDC status
public
Submitter Id
E-MTAB-10223:do18191_3
age
3
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
individual
-
organism part
liver
ploidy
4n
sample name
E-MTAB-10223:do18191_3
sex
male
strain
C57BL6/J