049-blank
extraction blank
collection_date
NA
depth
NA
elev
NA
env_broad_scale
NA
env_local_scale
NA
env_medium
NA
geo_loc_name
NA
lat_lon
NA
annual_season_precpt
NA
annual_season_temp
NA
cur_land_use
NA
cur_vegetation
NA
cur_vegetation_meth
NA
drainage_class
NA
extreme_event
NA
extreme_salinity
NA
fao_class
NA
local_class
NA
local_class_meth
NA
rel_to_oxygen
NA
samp_collect_device
NA
samp_mat_process
NA
samp_size
NA
sieving
NA
soil_type
NA
soil_type_meth
NA
store_cond
NA
mixing_set
not applicable
times_mixed
NA
Fwd_Primer_Barcode
TCATCGAG
Rev_Primer_Barcode
TCATAGAC
seq_meth
Illumina MiSeq 2x250 PE
env_material
NA
nucl_acid_ext
Qiagen DNeasy PowerLyzer PowerSoil DNA extraction kit
target_gene
16S rRNA
target_subfragment
V4
pcr_cond
initial denaturation:98_2; denaturation:98_0.167; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil