Skin hair follicle stem cells separated from DTG mice backskin by FACS sorting. Biological replicate 1. Mice were treated by Dox administration for 8 hours. Skins were digested in Trypsin-EDTA (Life technology) at 4 overnight. Epithelium cells were then released by scraping the epidermal side and filtered through 40 m cell strainers. The cell suspensions were incubated with PE-CD49f and FITC-CD34 for 30 min on ice and DAPI was used to exclude dead cells. CD49f+CD34+live cells were separated and collected.
breed
FVB
age
7week
sex
female
tissue
hair follicle stem cell
biomaterial_provider
Zhang Lab,CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
sample_id
19
BioSampleModel
Model organism or animal