day_12_not_edited_rep_2
Protocols: Samples were harvested for iPSCs prior to induction of organoid formation as well as at days 5, 7, 9, 11, 12, and 21 after induction of organoid formation (according to 10.1038/nbt.3392).Total RNA was collected. Parental Cell line:WT29-iCas9 iPSCs were infected with lentiviruses containing an expression construct for WT1 gRNA and Puromycin at a multiplicity of infection of 0.5, and were selected for 6 days. No Doxycyline was added RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)
ENA first public
2021-08-01
ENA last update
2021-08-01
External Id
SAMEA7745722
INSDC center alias
Friedrich Miescher Institute for Biomedical Research
INSDC center name
Friedrich Miescher Institute for Biomedical Research
INSDC first public
2021-08-01T12:14:03Z
INSDC last update
2021-08-01T12:14:03Z
INSDC status
public
Submitter Id
E-MTAB-9957:day_12_not_edited_rep_2
broker name
ArrayExpress
cell line
WT29-iCas9
cell type
induced pluripotent stem cell
common name
human
genotype
control, not edited
growth condition
organoid culture
sample name
E-MTAB-9957:day_12_not_edited_rep_2