16S rRNA Seq of rainbow trout fry gut: fish fed with florfenicol-coated feed one day prior to infection (day -1 _fed with control feed at this time point) replicate3
host
Oncorhynchus mykiss
isolation_source
intestine
collection_date
2019-09-18
geo_loc_name
Denmark
lat_lon
not applicable
therapy
Florfenicol-coated feed
ongoing_therapy
NO (control feed)
bacterial_challenge_with_Flavobacterium_psychrophilum
NO
sampling_day_(day0=bacterial_challenge;_dpi=days_post_infection)
day -1
replicate
biological replicate 3
re-isolation_of_pathogen_in_sampled_fish
NO
samp_mat_process
The distal part of the intestine was aseptically removed together with the fecal content (if present), placed in a sterile 1.5 ml micro tube and stored at -20C. DNA extraction was performed using the Maxwell LEV Blood DNA Purification Kit. A community analysis targeting the 16S rRNA gene (V3-V4 region) of the DNA extracted was then performed.
BioSampleModel
Metagenome or environmental