RNA-seq_dex_etoh_rep1
Protocols: RNA was isolated from 1 million cells using the RNeasy kit from Qiagen. A549 cells were grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated with either 100 nM dexamethasone or 0.1% ethanol. After 4 hours, cells were washed 2x with PBS and cultured in hormone-free medium for 24 hours, after which cells were treated again for 4 hours with either 100 nM dexamethasone or 0.1% ethanol. Ribosomal RNA was depleted using the KAPA RNA HyperPrep with RiboErase (Roche #08098131702). Sequencing libraries were prepared using KAPA RNA HyperPrep with RiboErase (Roche #08098131702).
ENA first public
2021-05-12
ENA last update
2021-05-12
External Id
SAMEA7725471
INSDC center name
MPIMG
INSDC first public
2021-05-03T00:22:16Z
INSDC last update
2020-12-16T15:09:16Z
INSDC status
public
Submitter Id
E-MTAB-9923:RNA-seq_dex_etoh_rep1
broker name
ArrayExpress
cell line
A549
common name
human
developmental stage
adult
disease
carcinoma
organism part
lung
sample name
E-MTAB-9923:RNA-seq_dex_etoh_rep1