Cut&Run H3K27Ac Bulk
Protocols: H3K27Ac Cut&Run libraries for the MUTZ3 bulk and sorted fragments were generated with an input of 200.000 cells. The MUTZ3 cell lines were cultured in αMEM (HyClone) with 20% fetal calf serum (FCS) and 20% conditioned 5637 medium. The 293T were cultured in DMEM (Gibco) with 10% FCS. All cell lines were supplemented with 50 U/mL penicillin and 50 μg/mL streptomycin. Viable frozen AML cells were thawed and resuspended in IMDM medium supplemented with: 20% BIT medium (Stem cell technologies), 1x β-mercaptoethanol (1000x Life technologies), 6 µg/ml LDL (Sigma Aldrich), human IL6, IL3, G-CSF, GM-CSF at 20 ng/ml and FLT3, SCF at 50 ng/ml (Peprotech. The protocol described by the Henikoff group was used to generate these tracks (Skene et al., 2018), using a 0.04% Digitonin buffer and with the addition of cOmplete, EDTA-free Protease Inhibitor Cocktail (Roche) and 1M Sodiumbutyrate (Sigma Aldrich) to all the buffers. Isolation was done according to the standard Phenol Chloroform protocol. Cut&Run samples were processed according to the protocol described by the Fazzio group
ENA first public
2021-07-26
ENA last update
2021-07-26
External Id
SAMEA7742617
INSDC center alias
Department of Hematology, Erasmus University Medical Center
INSDC center name
Department of Hematology, Erasmus University Medical Center
INSDC first public
2021-07-26T12:17:16Z
INSDC last update
2021-07-26T12:17:16Z
INSDC status
public
Submitter Id
E-MTAB-9946:Cut&Run H3K27Ac Bulk
broker name
ArrayExpress
cell line
MUTZ-3
cell type
myeloblast
common name
human
developmental stage
adult
disease
adult acute myeloid leukemia
genotype
inv(3)
organism part
bone marrow
sample name
E-MTAB-9946:Cut&Run H3K27Ac Bulk
sorted fraction
Bulk