202-16x
mixed trt sample, see times_mixed column
collection_date
2018-08-30
depth
15-20 cm
elev
430 m
env_broad_scale
woodland area[ENVO:00000109]
env_local_scale
woodland area[ENVO:00000109]
env_medium
not applicable
geo_loc_name
USA:Wisconsin
lat_lon
45.748 N 90.731 W
annual_season_precpt
840 mm
annual_season_temp
5 C
cur_land_use
hardwoods (e.g. oak,hickory,elm,aspen)
cur_vegetation
northern mesic forest, early-to-mid seral
drainage_class
moderately well
extreme_event
16 week lab incubation with physical mixing treatments
fao_class
Luvisols
local_class
Freeon silt loam
local_class_meth
USDA soil classification
rel_to_oxygen
aerobe
samp_collect_device
Metal core, 1.8 cm dia
samp_mat_process
4 C until sieving to 2mm and start of incubation at RT
samp_size
50 mg
sieving
2mm
soil_type
Freeon silt loam
soil_type_meth
USDA soil classification
store_cond
after 16 week incubation, samples were stored at -80 C for up to a month prior to extraction
mixing_set
K
times_mixed
16
Fwd_Primer_Barcode
ACGTCTCG
Rev_Primer_Barcode
TAGCAGAC
seq_meth
Illumina MiSeq 2x250 PE
env_material
soil
nucl_acid_ext
Qiagen DNeasy PowerLyzer PowerSoil DNA extraction kit
target_gene
16S rRNA
target_subfragment
V4
pcr_cond
initial denaturation:98_2; denaturation:98_0.167; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil