ATAC-seq_etoh_4h
Protocols: ATAC-seq was performed with 100,000 cells per treatment, cells were grown in DMEM containing 5% fetal bovine serum. A549 cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 4 hours with 100 nM demxathasone, 100 nM hydrocortisone or 0.1% ethanol and either harvested immediately or washed 2x with PBS and subsequently cultured in hormone-free medium for 24 hours before harvest. ATAC-seq was performed with 100,000 cells per treatment according to the Omni-ATAC-seq protocol [PMID: 28846090]. DNA was extracted using the DNA Clean & Concentrator Kit (Zymo research #D4014). Library amplification involved 11 PCR cycles using the NEBNext High-Fidelity 2x PCR Master Mix (New England Labs, Cat #M0541).
ENA first public
2021-05-12
ENA last update
2021-05-12
External Id
SAMEA7708251
INSDC center name
MPIMG
INSDC first public
2021-05-03T00:22:16Z
INSDC last update
2020-12-14T15:02:40Z
INSDC status
public
Submitter Id
E-MTAB-9911:ATAC-seq_etoh_4h
broker name
ArrayExpress
cell line
A549
common name
human
developmental stage
adult
disease
carcinoma
organism part
lung
sample name
E-MTAB-9911:ATAC-seq_etoh_4h