Total population
Protocols: MUTZ3 cells were harvested at the indicated time point after treatment. The MUTZ3 cell lines were cultured in αMEM (HyClone) with 20% fetal calf serum (FCS) and 20% conditioned 5637 medium. They were supplemented with 50 U/mL penicillin and 50 μg/mL streptomycin. The sgRNAs were either cloned into pLentiV2_U6-IT-mPgk-iRFP720 using BsmBI restriction sites, px330 using BbsI or were in vitro transcribed using the T7 promoter. Lentiviruses were prepared by transfecting 293T cells with lentiviral packaging constructs pSPAX2 / pMdelta2.G and sgRNA cloned into pLentiV2_U6-IT-mPgk-iRFP720. RNA was isolated either using Trizol or the Qiagen Allprep DNA/RNA kit and protocol (Qiagen, #80204). For RNA sequencing, sample libraries were prepped using 500 ng of input RNA according to the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche) using Unique Dual Index adapters (Integrated DNA Technologies, Inc.).
ENA first public
2021-07-26
ENA last update
2021-07-26
External Id
SAMEA7742158
INSDC center alias
Department of Hematology, Erasmus University Medical Center
INSDC center name
Department of Hematology, Erasmus University Medical Center
INSDC first public
2021-07-26T12:17:16Z
INSDC last update
2021-07-26T12:17:16Z
INSDC status
public
Submitter Id
E-MTAB-9939:Total population
broker name
ArrayExpress
cell line
MUTZ-3
cell type
myeloblast
common name
human
developmental stage
adult
disease
acute myeloid leukemia
genotype
inv(3)
organism part
bone marrow
phenotype
unsorted
sample name
E-MTAB-9939:Total population