ID-SNI-668-17-L
Protocols: Nuclei was isolated from frozen livers from young C57BL/6 mice (3 month old) using the protocol described in Krishnaswami et al. 2016 (Nature Protocols, 11: 499-524). Nuclei was purified using Iodixanol gradient (Optiprep, D1556, Sigma Aldrich Chemie). Nuclei were stained with Hoechst and FACS sorted according to their ploidy level diploid (2n) and tetraploid (4n) in 384-well plates. Sequencing libraries were prepared using Illumina Nextera XT kit (Illumina, #FC-131-1096) and the combination of 384 Combinatorial Dual Indexes (Illumina- Set A to D , #FC-131-2001 to FC-131-2004) following the manufacturer instructions but miniaturizing the reactions 10x using a liquid handling robot (Mosquito HV).
ENA first public
2021-05-07
ENA last update
2021-03-11
External Id
SAMEA8230564
INSDC center alias
Helmholtz Pioneer Campus
INSDC center name
Helmholtz Pioneer Campus
INSDC first public
2021-05-07T00:27:00Z
INSDC last update
2021-03-11T09:48:27Z
INSDC status
public
Submitter Id
E-MTAB-93331615455990:ID-SNI-668-17-L
age
3
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
individual
AN18CUK011304
organism part
liver
ploidy
4n
post analysis well quality
fail
sample name
E-MTAB-93331615455990:ID-SNI-668-17-L
sex
male
strain
C57Bl6/J