In vivo footprinting of Sir3 from a time course after exit from starvation uisng Sir3Dam and direct detection of GATC methylation by nanopore sequencing (Oxford Nanopore). Fast5 files basecalled with guppy basecaller and dna_r9.4.1_450bps_modbases_dam-dcm-cpg_hac.cfg. Multiplexed library mix with the Rapid Barcoding Kit (RBK401:Starvation; RBK402: 5min after release from starvation; RBK403: 15min after release from starvation; RBK405: 60min after release from starvation).
strain
Sir3Dam (MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 SIR3:: Myc9-DAM (methylase from E.coli) Phleomycin)
isolate
starvation and 5, 15 and 60min after exit from starvation (glucose depletion)
cultivar
not applicable
ecotype
not applicable
collection_date
not applicable
env_broad_scale
not applicable
env_local_scale
not applicable
env_medium
not applicable
geo_loc_name
not applicable
isol_growth_condt
not applicable
lat_lon
not applicable
BioSampleModel
MIGS.eu