Per biological replicate, cells were transduced at 750-1000X representation with the Digenic Paralog CRISPR Library in cell lines stably expressing S. Pyogenese Cas9. Cells were selected with puromycin for 3 days post-transduction and passaged for 16 days after selection. Genomic DNA was collected from these cells was PCR amplified and sequenced.
isolate
lentiviral integrate
age
78
biomaterial_provider
Genetic Perturbation Platform
sex
male
tissue
lymph_node
cell_line
MEWO
disease
Skin Cancer
treatment
N/A
DeplexedBarcode
AATCCACG
BioSampleModel
Human