Spleen_Fibro_R1
Protocols: Tissues were digested and CD45-CD31-Epcam-PDPN+PDGFRA+ (fibroblasts) or CD45-CD31-Epcam-PDPN+PDGFRA- mesothelial cells were sorted directly into TRIZOL. RNA from FACS sorted cells was sourced from the following mouse tissues: Liver, Lung, Lymph Node, Spleen, Bone, Pancreas, Omentum, Mesentery, Epididymal, Inguinal Adipose, Brown Adipose Tissue. RNA was isolated as described (Painter et al., 2011, J. Imm.) or at Expression Analysis, Inc. Paired-end RNA-seq libraries were constructed using the SMARTer Low Input RNA Kit (Takara.
ENA first public
2021-05-13
ENA last update
2021-05-13
External Id
SAMEA8491884
INSDC center name
GENENTECH INC.
INSDC first public
2021-05-04T00:42:28Z
INSDC last update
2021-03-31T14:33:19Z
INSDC status
public
Submitter Id
E-MTAB-10324:Spleen_Fibro_R1
age
6 to 12
broker name
ArrayExpress
cell type
fibroblast
common name
house mouse
developmental stage
adult
organism part
spleen
phenotype
CD45-CD31-Epcam-PDPN+PDGFRA+
sample name
E-MTAB-10324:Spleen_Fibro_R1
sex
male
strain
C57BL6/J