Per biological replicate, cells were transduced at 750-1000X representation with the Digenic Paralog CRISPR Library in cell lines stably expressing S. Pyogenese Cas9. Cells were selected with puromycin for 3 days post-transduction and passaged for 16 days after selection. Genomic DNA was collected from these cells was PCR amplified and sequenced.
isolate
lentiviral integrate
age
999
biomaterial_provider
Genetic Perturbation Platform
sex
N/A
tissue
N/A
cell_line
pDNA
treatment
N/A
DeplexedBarcode
TTCTCATA
BioSampleModel
Human