Jetty3-2-t2-LDPE-8-2019-06-06
ENA first public
2021-07-24
ENA last update
2021-07-24
External Id
SAMEA8892237
INSDC center alias
NIOZ
INSDC center name
Royal Netherlands Institute for Sea Research
INSDC first public
2021-07-24T00:16:48Z
INSDC last update
2021-07-24T00:16:48Z
INSDC status
public
Submitter Id
NIOZ165.85
ammonia
5.049 microMol/L
barcodenames
515F.Golay169.926R.BC170.fasta
chlorophyll
0
collection date
2019-06-06
dissolved inorganic carbon
2316.2
environment (biome)
marine biome (ENVO: 00000428)
environment (feature)
manufactured product (ENVO: 00003074)
environment (material)
low density polyethylene
experimental factor
time series
forwardprimersequence
GTGYCAGCMGCCGCGGTAA
geographic location (country and/or sea)
Netherlands
geographic location (latitude)
53.002
geographic location (longitude)
4.789
investigation type
mimarks-survey
miscellaneous environmental package
miscellaneous natural or artificial environment
nitrate
7.628
nitrogen
29.85
nucleic acid extraction
Gentra Puregene Tissue DNA Isolation kit
oxygenation status of sample
aerobic
pcr conditions
initial denaturation at 98 C for 30 s; 30 cycles of 98 C for 30 s, 50 C for 15 s, and 72 C for 30 s; final extension at 72 C for 5 minutes
pcr primers
Forward Primer (515F) GTGYCAGCMGCCGCGGTAA Reverse Primer (926R) CCGYCAATTYMTTTRAGTTT
phosphate
0.174
phosphorus
0.523 microMol/L
project name
NIOZ Jetty Density Experiment
purificationmethod
AmpureBeads
reverseprimersequence
CCGYCAATTYMTTTRAGTTT
salinity
30.5
sample name
NIOZ165.85
sample storage temperature
-20
secchi depth
2.4 m
sequence quality check
software
sequencing method
Illumina MiSeq
silicate
0.94
target gene
16S rRNA
target subfragment
V4V5
targetedampliconsize
600bp400bp
temperature
16.4
total suspended matter
6.6 mg/L