Untagged_V5_Rep3 [CUT&RUN]
source_name
Untagged
cell line/type
E14 mES cell line
tag integration
osTIR1 was integrated into the genome using CRISPR-directed homologous recombination
nuclear harvest
Cells were trypsinized, quenched, pelleted, washed with cold PBS, and nuclei were extracted
primary antibody
anti-V5 mouse monoclonal, Invitrogen 46-0705, lot 1923773
secondary antibody
recombinant Protein A/Protein G- MNase (pA/G-MN)