set57.WT.2.RNA-seq.rep1
source_name
human colorectal carcinoma cell line
cell line
HCT116
genotype
Wild type
treatment
Cells were treated with 100µg/mL cycloheximide for 10min and then lysed in polysome lysis buffer. After centrifugation, 100 µL cell lysates were used to extract RNA with TRIzol. Then 5~10 µg RNA was used to purify mRNAs with oligo dT beads. The purified mRNAs were used for RNA-seq library preparation.
molecule
polyA RNA