SPT16_AID_V5_Clone2_Untargeted_Rep1 [CUT&RUN]
source_name
SPT16_AID_V5_Clone_2
cell line/type
E14 mES cell line
tag integration
osTIR1 was integrated into the genome using CRISPR-directed homologous recombination; separately, Supt16 was C-terminally tagged with the 37 kDa mini-AID tag and a triple V5 tag
nuclear harvest
Cells were trypsinized, quenched, pelleted, washed with cold PBS, and nuclei were extracted
primary antibody
None
secondary antibody
recombinant Protein A/Protein G- MNase (pA/G-MN)