Nov_N03_0020_50_200_ni_hi
ENA first public
2021-08-17
ENA last update
2021-08-17
External Id
SAMEA7579395
INSDC center alias
UIO
INSDC center name
University of Oslo, Norway
INSDC first public
2021-08-17T00:25:44Z
INSDC last update
2021-08-17T00:25:44Z
INSDC status
public
Submitter Id
MP5_MI_29_euk_hiseq
amount or size of sample collected
25
chlorophyll
0.076
collection date
2014-11-17
environment (biome)
Ocean
environment (feature)
Open Ocean
environment (material)
plankton
fraction_max
200
fraction_min
50
geographic location (country and/or sea)
Svalbard
geographic location (depth)
20
geographic location (latitude)
79.022
geographic location (longitude)
11.33
geographic location (region and locality)
Arctic Ocean
investigation type
eukaryote
nucleic acid amplification
KAPA HiFi HotStart ReadyMix 2x
nucleic acid extraction
DNeasy Plant mini kit
pcr primers
F: 5'-CCAGCASCYGCGGTAATTCC-3', R: 5'-ACTTTCGTTCTTGATYRATGA-3' (Piredda et al. 2017)
project name
MicroPolar
salinity
34.59
sample collection device or method
Niskin bottle
sample material processing
filtration
sample name
MP5_MI_29_euk_hiseq
sample volume or weight for DNA extraction
25000
sequencing method
Illumina HiSeq
target gene
18S rDNA
target subfragment
V4
temperature
2.3
total depth of water column
327
water environmental package
water