Untagged_IAA_Rep3 [MNase-seq]
source_name
Untagged_IAA
cell ine/type
E14 mES cell line
tag integration
osTIR1 was integrated into the genome using CRISPR-directed homologous recombination
treatment
Cells were left undisturbed on 10 cm plates for 48 hours prior to treatment. Cells were treated with 500 nM 3-IAA mixed into medium for 24 hours. Cells were incubated at 37 degrees Celsius with 5% CO2 for the duration of treatment.
extraction
Cells were trypsinized, quenched, pelleted, washed with cold PBS, and crosslinked with 1% formaldehyde for 15 minutes. Crosslinking was quenched with .5M glycine for 5 minutes. Fixed cells were pelleted and washed with cold PBS