Protocols: Induced pluripotent stem cells were subjected to B-cell differentiation as described in Richardson et al, 2021 https://doi.org/10.1016/j.xpro.2021.100420. Cells were harvested and subjected to CD45 MACS enrichment according to manufacturer's instructions (Miltenyi Biotec, 130-045-801). https://doi.org/10.1016/j.xpro.2021.100420 Cells were crosslinked with 1% formaldehyde and sonicated to yield chromatin of 100-500 bp. ChIP was performed by as previously described (May et al., 2013) using antibodies: RUNX1 (Abcam, ab23980), V5 (Abcam, ab9116). Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645)
ENA-FIRST-PUBLIC
2022-10-31
ENA-LAST-UPDATE
2022-10-31
External Id
SAMEA111533045
INSDC center alias
Cancer Institute University College London
INSDC center name
Cancer Institute University College London
INSDC first public
2022-10-31T16:00:03Z
INSDC last update
2022-10-31T16:00:03Z
INSDC status
public
Submitter Id
E-MTAB-12208:ER2.1_RUNX1
broker name
ArrayExpress
cell_line
ER2.1
cell_type
B cell
common name
human
disease
B-cell acute lymphoblastic leukemia
genotype
V5-ETV6-RUNX1
immunophenotype
CD45-positive
organism part
blood
progenitor cell type
induced pluripotent stem cell
sample name
E-MTAB-12208:ER2.1_RUNX1