collection_date
2021-03-22
env_broad_scale
zebrafish facility
env_local_scale
not applicable
env_medium
egg water
geo_loc_name
Netherlands: Leiden
host
Danio rerio
lat_lon
52.17 N 4.46 E
host_age
3 days post-fertilization
host_family_relationship
A23
host_genotype
ABxTL
host_growth_cond
standard (http://zfin.org)
host_life_stage
larvae
host_phenotype
wildtype
host_taxid
7955
host_tissue_sampled
whole body
organism_count
30
perturbation
Conventionalized
samp_mat_process
Larvae were washed thrice in sterile egg water and stored at -80 dC in 0.8 mL lysis buffer until DNA extraction. Immediately prior to DNA extraction (DNeasy Blood and Tissue Kit), the sample was thawed and homogenized for 5 min with 6 zirconium oxide beads (1 mm-diameter) in a tissue homogenizer (speed 7, Bullet Blender Blue-CE, Next Advance). For remaining steps, the extraction protocol of Davis et al. (2016) was followed (DOI: 10.1016/j.dib.2016.06.057).
samp_size
1
samp_store_dur
8 days
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.host-associated