Protocols: The CD41+/CD42+ MK were sorted (10 000 to 30 000 cells). Cells were submitted to cell lysis, transposition, and purification steps according to Illumina protocol. The transposed DNA fragments were amplified by PCR 12 times using adapters from the Nextera Index Kit (Illumina). PCR purification was performed using MinElute PCR Purification Kit (Qiagen, 28004) to remove large fragments and remaining primers. Library quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies 5067-4626).
ENA first public
2022-06-15
ENA last update
2022-06-15
External Id
SAMEA10379572
INSDC center alias
INSERM Gustave Roussy institute
INSDC center name
INSERM Gustave Roussy institute
INSDC first public
2022-06-15T12:17:01Z
INSDC last update
2022-06-15T12:17:01Z
INSDC status
public
Submitter Id
E-MTAB-11044:S5_2_S8
broker name
ArrayExpress
cell type
CD34-negative, CD41-positive, CD42-positive megakaryocyte cell
common name
human
developmental stage
embryo
disease
Down syndrome
genotype
SMC3 mutation
karyotype
trisomy
organism part
embryo
progenitor cell type
induced pluripotent stem cell
sample name
E-MTAB-11044:S5_2_S8