Protocols: Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies).
ENA-FIRST-PUBLIC
2022-08-02
ENA-LAST-UPDATE
2022-08-02
External Id
SAMEA8136064
INSDC center alias
KIS
INSDC center name
Karolinska Institutet
INSDC first public
2022-08-02T00:25:18Z
INSDC last update
2022-08-02T00:25:18Z
INSDC status
public
Submitter Id
E-MTAB-10097:D10_38
age
7
broker name
ArrayExpress
common name
human
developmental stage
embryo stage
individual
D10
inferred lineage
mural
organism part
whole organism
sample name
E-MTAB-10097:D10_38
sex
female
single cell well quality
OK
stimulus
Treat