Wolbachia is an obligate endosymbiont that cannot be cultivated outside the host and often present at a relatively low abundance, it can be difficult to extract Wolbachia DNA directly from a host. In previous studies, we transfected wTtru that naturally infects Tetranychus truncatus into Aedes albopictus cell line, where it maintained relatively high titers. A higher quality Wolbachia genome was obtained by purifying Wolbachia from this cell line.
host
Tetranychus truncatus
collection_date
2012
geo_loc_name
China: Anhui
sample_type
cell culture
isolate
AH2012_wTtru
strain
missing
BioSampleModel
Microbe, viral or environmental