Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4-OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease-free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo-Zero Gold
ENA-FIRST-PUBLIC
2022-10-31
ENA-LAST-UPDATE
2022-10-31
External Id
SAMEA111469062
INSDC center alias
Department of Life Sciences University of Bath
INSDC center name
Department of Life Sciences University of Bath
INSDC first public
2022-10-31T00:16:51Z
INSDC last update
2022-10-31T00:16:51Z
INSDC status
public
Submitter Id
E-MTAB-12301:16261X10
age
28
broker name
ArrayExpress
common name
zebrafish
developmental stage
embryo
disease
normal
genotype
cyt-hSNRNP70/-
organism part
whole organism
sample name
E-MTAB-12301:16261X10