wildtype and mutant recombinant A/WSN/1933(H1N1) viruses were rescued and passaged to P3 at 37oC, 5% CO2, and MOI 0.001, the viral RNA was extracted, and genome segment amplicons were amplifed by consensus primers MBTuni-12 and MBTuni-13, the PCR prodcuts were constructed in to library
strain
A/WSN/1933(H1N1)
collected_by
Xinzhou XU
collection_date
2021-04
geo_loc_name
not applicable
host
Homo sapiens
host_disease
Influenza, Human
isolation_source
recombinant plasmids
lat_lon
not applicable
genotype
mutant
host_description
MDCK CELLS
passage_history
passage 3 on MDCK cells at MOI 0.001
subtype
H1N1
replicate
biological replicate 2
lane
sequencing replicate 2
sample ID
8
BioSampleModel
Pathogen.cl