source_name
Pancreatic spheroids
cell type
Pancreatic epithelial cells
growth protocol
DMEM/F12 1:1 supplemented with 2.5 mM L-Glutamine, 15 mM HEPES Buffer (HyClone), 5 mg/ml D-glucose (Sigma Aldrich), 1.22 mg/ml nicotinamide (Sigma Aldrich), 5 nM 3,3,5-Tri-iodo-L-thyronine (Sigma Aldrich), 0.5 μM hydrocortisone Solution (Sigma Aldrich), 100 ng/ml cholera toxin (Sigma Aldrich), 0.5 % insulin-transferrin-selenium (Corning), 100 μg/ml penicillin/streptomycin (Gibco), 0.1 mg/ml soybean trypsin inhibitor (Sigma Aldrich), 20 ng/ml EGF (Peprotech), 25 μg/ml bovine pituitary extract (Invitrogen), and 5% Nu Serum IV Culture Supplement (BD)
antibody
none
treatment
Untreated