Protocols: Cells were sorted to high purity for transgene expression (GFP), expanded in vitro, and approximately 10^8 cells harvested for subsequent ChIP experiments. Cells were crosslinked with 1% formaldehyde and sonicated to yield chromatin of 100-500 bp. ChIP was performed by as previously described (May et al., 2013) using antibodies: H3K27ac. See May et al, 2013. https://doi.org/10.1016/j.stem.2013.09.003
ENA-FIRST-PUBLIC
2022-10-31
ENA-LAST-UPDATE
2022-10-31
External Id
SAMEA111455300
INSDC center alias
Cancer Institute University College London
INSDC center name
Cancer Institute University College London
INSDC first public
2022-10-31T00:16:52Z
INSDC last update
2022-10-31T00:16:52Z
INSDC status
public
Submitter Id
E-MTAB-12207:TARG_K27ac_R1
broker name
ArrayExpress
cell_line
NALM6
cell_type
B-lymphoblast
common name
human
disease
B-cell acute lymphoblastic leukemia
genotype
ETV6-RUNX1-R139G
organism part
blood
progenitor cell type
induced pluripotent stem cell
sample name
E-MTAB-12207:TARG_K27ac_R1