ENA-FIRST-PUBLIC
2022-10-13T12:28:57Z
ENA-LAST-UPDATE
2022-10-13T12:28:57Z
External Id
SAMEA111418863
INSDC center name
WAGENINGEN UNIVERSITY
INSDC first public
2022-10-13T12:28:57Z
INSDC last update
2022-10-13T12:28:57Z
INSDC status
public
Metadata submission software
FAIR Data Station
Submitter Id
Ppu_r12_2
biosafety level
1
observation_unit description
In order to investigate the off-target mutations that occur in the cells during the process of recombineering when using each individual SSAP, we used the GenElute Bacterial Genomic DNA kit (Sigma-Aldrich) to isolate gDNA from two colonies of the next strains: (i) P. putida expressing pSEVA2514-R8-mutL, (ii) P. putida expressing pSEVA2514-R12-mutL, (iii) P. putida expressing pSEVA2514-R35-mutL, (iv) P. putida expressing pSEVA2514-R47-mutL, (v) P. taiwanensis expressing pSEVA2514-R47-mutL, (vi) P. fluorescens expressing pSEVA2514-R9-mutL, (vii) P. fluorescens expressing pSEVA2514-R47-mutL, (viii) P. aeruginosa expressing pSEVA6514-R8-mutL. Successful incorporation of an oligonucleotide mediating either the rpsL gene mutation in P. putida, P. taiwanensis and P. fluorescens, or the rpoB gene mutation in P. aeruginosa had been performed using one recombineering cycle in the aforementioned strains prior gDNA isolation. In the same way, but without undertaking any recombineering, we extracted the gDNA from one colony of each four wild type parental strains.
observation_unit identifier
Ppu_r12_2
observation_unit name
P. putida expressing pSEVA2514-R12-mutL
packageIdentifier
UNLOCK_CORE
packageName
Core
sample description
P. putida expressing pSEVA2514-R12-mutL
sample identifier
Ppu_r12_2
sample name
P. putida expressing pSEVA2514-R12-mutL
scientific_name
Pseudomonas putida KT2440
scientific_name
P. putida
tax_id
160488
component_organism
Pseudomonas putida
component_organism
Pseudomonas putida KT2440