AQEPLR2_2017_sediment_12S_fishes
collection_date
2017-09-26
depth
top 2cm of stream bottom sediment at a water depth of 300 mm or less
env_broad_scale
freshwater river
env_local_scale
freshwater river
env_medium
stream sediment
geo_loc_name
USA:Great Smoky Mountains National Park
lat_lon
35.65 N 83.56 W
pcr_primers
FWD(MiFish_FW_F):5_GTCGGTAAAACTCGTGCCAGC, REV(MiFish_FW_R):5_CATAGTGGGGTATCTAATCCCAGTTTG
target_gene
Mitochondrial 12S gene
seq_meth
Illumina MiSeq with v3 chemistry generating paired end reads
nucl_acid_ext
Qiagen Powersoil kit using aproximately 100 mg of sediment following the manufacturer instructions.
Lab_ID
80
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water