Overexpession line grown on N-deficient medium.
ENA-FIRST-PUBLIC
2021-09-27
ENA-LAST-UPDATE
2021-09-27
External Id
SAMEA8961907
INSDC center alias
Bioscience and Biotechnology Institute of Aix-Marseille (BIAM)
INSDC center name
Bioscience and Biotechnology Institute of Aix-Marseille (BIAM)
INSDC first public
2021-09-27T00:27:31Z
INSDC last update
2021-09-27T00:27:31Z
INSDC status
public
Submitter Id
OXRSH1_N50_2
air temperature regimen
22°C/19.5°C day/night
amount or size of sample collected
~300 mg
collection date
2019-11-29
common name
thale cress
culture rooting medium
Control media diluted 1/25 in 0.5× Murashige and Skoog salts without nitrogen
estimated size
135 Mb
geographic location (country and/or sea)
France
geographic location (latitude)
43.2315625
geographic location (longitude)
5.4393125
growth facility
growth chamber
isolation and growth condition
xxxxx
light regimen
16-h-light/8-h-dark photoperiod at 80 µmol m−2 s−1 PAR lighting
number of replicons
5
organism common name
Arabidopsis
plant developmental stage
1.02
plant growth medium
in vitro plates
plant structure
aerial parts of seedlings
plant treatment
N-deprivation
ploidy
diploid
rooting conditions
Square plates containing 45 ml growth medium and 100 equally spaced seedlings. Sealed with microporous tape.
rooting medium carbon
1% sucrose
rooting medium macronutrients
CaCl2, 1.5 mM; KH2PO4, 0.63 mM; KNO3 0.38 mM; MgSO4, 0.75 mM; NH4NO3, 0.41 mM; KCl, 4.63 mM; K2SO4, 4. 58mM
rooting medium micronutrients
0.5X MS micronutrients
rooting medium pH
pH5.7 (KOH)
rooting medium regulators
MES buffer (0.5g/l)
rooting medium solidifier
0.4% phytagel
sample collection device or method
snap freezing in liquid nitrogen
sample health state
healthy
sample name
OXRSH1_N50_2
sampled age
12 days
sampling time point
14h00
source material identifiers
xxxxx
subspecific genetic lineage rank
Ecotype Col-0