Pond 6 replica e
ENA first public
2021-12-07
ENA last update
2021-12-07
External Id
SAMEA11322895
INSDC center alias
UNIVERSITY OF EXETER
INSDC center name
University of Exeter
INSDC first public
2021-12-07T00:33:34Z
INSDC last update
2021-12-07T00:33:34Z
INSDC status
public
Submitter Id
P6e
amount or size of sample collected
0.2
collection date
2017-10
environment (biome)
freshwater biome (ENVO:00000873)
environment (feature)
pond (ENVO:00000033)
environment (material)
fresh water (ENVO:00002011)
geographic location (country and/or sea)
Malawi
geographic location (depth)
0
geographic location (latitude)
not collected
geographic location (longitude)
not collected
geographic location (region and locality)
Maldeco, Mangochi
investigation type
mimarks-survey
nucleic acid extraction
https://dx.doi.org/10.17504/protocols.io.bw8gphtw
pcr conditions
98 �C for 30 seconds; 30 cycles of 98 �C for 10 seconds, 55 �C for 30 seconds, 72 �C for 30 seconds; and a final extension of 72 �C for 2 minutes
pcr primers
515F (5'-GTGYCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACNVGGGTWTCTAAT-3')
project name
Tilapia skin microbiomes Malawi
sample collection device or method
0.4 um polycarbonate filter
sample material processing
stored in ethanol
sample name
P6e
sequencing method
Illumina MiSeq
target gene
16S rRNA
target subfragment
V4
water environmental package
water