18S - Pond 5 replica a
ENA first public
2021-12-07
ENA last update
2021-12-07
External Id
SAMEA11324745
INSDC center alias
UNIVERSITY OF EXETER
INSDC center name
University of Exeter
INSDC first public
2021-12-07T00:33:34Z
INSDC last update
2021-12-07T00:33:34Z
INSDC status
public
Submitter Id
18S:P5a
amount or size of sample collected
0.2
collection date
2017-10
environment (biome)
freshwater biome (ENVO:00000873)
environment (feature)
pond (ENVO:00000033)
environment (material)
fresh water (ENVO:00002011)
geographic location (country and/or sea)
Malawi
geographic location (depth)
0
geographic location (latitude)
not collected
geographic location (longitude)
not collected
geographic location (region and locality)
Blantyre
investigation type
mimarks-survey
nucleic acid extraction
https://dx.doi.org/10.17504/protocols.io.bw8gphtw
pcr conditions
98 �C for 30 seconds; 30 cycles of 98 �C for 10 seconds, 60 �C for 30 seconds, 72 �C for 30 seconds; and a final extension of 72 �C for 2 minutes
pcr primers
1391F (5'-GTACACACCGCCCGTC-3') and EukBr (5'-TGATCCTTCTGCAGGTTCACCTAC-3')
project name
Tilapia skin microbiomes Malawi
sample collection device or method
0.4 um polycarbonate filter
sample material processing
stored in ethanol
sample name
18S:P5a
sequencing method
Illumina MiSeq
target gene
18S rRNA
target subfragment
V9
water environmental package
water