Tilapia 6 Pond 3
ENA first public
2021-12-07
ENA last update
2021-12-07
External Id
SAMEA11325631
INSDC center alias
UNIVERSITY OF EXETER
INSDC center name
University of Exeter
INSDC first public
2021-12-07T00:33:34Z
INSDC last update
2021-12-07T00:33:34Z
INSDC status
public
Submitter Id
18S:T16s
collection date
2017-10
environment (biome)
freshwater biome (ENVO:00000873)
environment (feature)
pond (ENVO:00000033)
environment (material)
skin environment (ENVO:2100003)
geographic location (country and/or sea)
Malawi
geographic location (latitude)
not collected
geographic location (longitude)
not collected
geographic location (region and locality)
Blantyre
host body product
skin mucous
host body site
fish skin
host common name
Redbreast tilapia
host diet
omnivorous
host disease status
healthy
host length
15.1
host taxid
8129
host-associated environmental package
host-associated
investigation type
mimarks-survey
nucleic acid extraction
https://dx.doi.org/10.17504/protocols.io.bw8gphtw
pcr conditions
98 �C for 30 seconds; 30 cycles of 98 �C for 10 seconds, 60 �C for 30 seconds, 72 �C for 30 seconds; and a final extension of 72 �C for 2 minutes
pcr primers
1391F (5'-GTACACACCGCCCGTC-3') and EukBr (5'-TGATCCTTCTGCAGGTTCACCTAC-3')
project name
Tilapia skin microbiomes Malawi
sample collection device or method
swab
sample material processing
stored in RNAlater
sample name
18S:T16s
sequencing method
Illumina MiSeq
target gene
18S rRNA
target subfragment
V9