16S rRNA gene sequencing of cecal and colonic contents derived from SPF C1qc-/- pups fostered by FBZ treated dams (n=5)
Total genomic DNA from cecal and colonic contents derived from 20-day-old specific germ free (SPF) C1qc-/- mice fostered by dams treated with Fenbendazole (FBZ) during the lactation period, was extracted using QIAamp DNA Stool Mini Kit (QIAGEN). The V4 region of 16S rRNA gene were amplified with the published primer pair of 515F and 806R, followed by sequencing using an Ion S5 XL platform and 400 bp single-end reads were generated.