Validation of sg19 targeting BCL2 with NG-ABE8e in MOLM-13 cells, Venetoclax condition, day 21, replicate B
MOLM-13 cells were transduced with sg19 cloned into a vector containing ABE8e and a puromycin resistance cassette. Cells were selected with puromycin from day 2 post-transduction to day 8 post-transduction, then passaged in the presence of Venetoclax for the remainder of the screen. At 21 days post-transduction, cells were collected; genomic DNA from these cells was isolated, and the guide target sequences were PCR-amplified and sequenced.