Validation of empty vector control (RDA336, MM2, F_D1_R_B1) targeting BRCA1 with NG-CBE in HAP1 cells, Dropout condition, day 21, replicate B
HAP1 cells were transduced with empty vector control (RDA336, MM2, F_D1_R_B1) cloned into a vector containing CBE and a puromycin resistance cassette. Cells were selected with puromycin from day 2 post-transduction to day 8 post-transduction, then passaged in the absence of drug for the remainder of the screen. At 21 days post-transduction, cells were collected; genomic DNA from these cells was isolated, and the guide target sequences were PCR-amplified and sequenced.