EcTopoI ChIP-Seq, EcTopoI 14-kDa CTD overexpression, replicate 2
source_name
Escherichia coli DY330 topA-SPA
strain
DY330 topA-SPA
growth phase
Exponential phase
optical density at 600 nm
0.6
treatment
Overexpression of EcTopoI 14-kDa CTD was induced for 1 h with 1 mM IPTG (CTD)
immunoprecipitation reagent
ANTI-FLAG M2 affinity gel (Sigma-Aldrich)