MZ-2
isolate
the intestinal tissues of Eimeria necatrix infection chickens
breed
yellow-feathered chickens
host
chicken
isolation_source
sixty fifteen-day-old yellow-feathered chickens were infected with 1.0 104 sporulated E. necatrix oocysts. Infected intestinal tissues were removed after 136 h, cut longitudinally and rinsed three times with ice-cold PBS at pH 7.4. The mucosa was scraped using two glass slides and put into ten volumes of a solution containing 120 mM NaCl, 10 mM CaCl2, 3 mM K2HPO4, 20 mM Tris-HCl, 0.1% bovine serum albumin and 0.1% hyaluronidase and incubated for 1 h at 37 C in a thermostat water bath. Large intestinal debris was removed by filtering through gauze, and small debris was removed by sequential filtration through 17-m and 10-m polymon mesh (Sefar Filtration Solution Co. Ltd., Suzhou, China). The mixture was then centrifuged at 1400g for 10 min and the pellet was washed three times in ice-cold PBS. To remove red blood cells, the pellet was resuspended in lysis buffer (Solarbio, Beijing, China) and placed for 10 min at 4 C. After three washes with ice-cold PBS, the resulting MZ-2 were purified by density-gradient centrifugation using the method described by Mo et al.
collection_date
2020-10-26
geo_loc_name
China: Yangzhou
tissue
intestinal tissues
BioSampleModel
Invertebrate