late_elongating_pool_rep1
cultivar
Lea
dev_stage
3-months old plants
geo_loc_name
not applicable
tissue
young leaves
collected_by
O.W. Milvang, NMBU
collection_date
2015-12
growth_protocol
Seeds of red clover (Trifolium pratense L., cultivar 'Lea' (Graminor Ltd.)) were sown in a greenhouse in September 2015 and grown at approximately 16 °C with a 20 h photoperiod (natural light supplied with 90 µmol m-2 s-1 PAR (HPQ/HTI-P lamps)). The number of days from sowing until the first elongating internode was 2 cm long (days to elongation, DTE) was recorded for each plant.
phenotype
late elongating
population
subpopulation of cultivar Lea; first pool containing 17 late elongating plants
sample_type
The 52 earliest and 52 latest individuals (excluding non-elongating individuals) were selected for genotyping by pool-GBS. Pools were created as follows: DNA was extracted from leaf tissue of each individual with the DNeasy 96 Plant kit (Qiagen). The 52 individuals in each of the two phenotypic groups (early, late) were randomly divided into three subgroups of 17-18 individuals and equal amounts of DNA from each individual in each subgroup was combined in a pool, creating a total of six DNA pools. This pool contains the first subgroup (replicate 1) of 17 late elongating individuals.
BioSampleModel
Plant