CRC6
Protocols: Fresh nephrectomy samples were collected from a total of 5 untreated ccRCC patients. Up to 9 punch biopsies were collected in each specimen in order to capture its heterogeneity. Out of these 5 patients, 7 samples were obtained. Two samples consisted of fresh versus frozen single cells from the primary tumor site of one patient. Two other samples consisted of matched primary and distant thrombus sites (the vena cava) of a second patient. The three remaining samples came from the primary tumor sites of three distinct ccRCC patients. All steps for the nucleic acid extraction were carried out according to the manufacturer's protocol (Chromium™ Single Cell 3' Library & Gel Bead Kit v2 ; 10x Genomics) All steps for the construction of the single-cell libraries were carried out according to the manufacturer's protocol (Chromium™ Single Cell 3' Library & Gel Bead Kit v2 ; 10x Genomics)
ENA first public
2022-02-15
ENA-LAST-UPDATE
2022-08-01
External Id
SAMEA12287003
INSDC center alias
INSERM UMR1085
INSDC center name
INSERM UMR1085
INSDC first public
2022-02-15T00:13:16Z
INSDC last update
2022-08-01T10:42:13Z
INSDC status
suppressed
Submitter Id
E-MTAB-11310:CRC6
age
70
broker name
ArrayExpress
cell type
kidney cell
common name
human
developmental stage
adult
disease
clear cell renal carcinoma
disease staging
T3a tumor stage
individual
RCC4
organism part
kidney
sample name
E-MTAB-11310:CRC6
sampling site
Sample obtained from radical nephrectomy (dimension 23*13*7)
sex
female
specimen with known storage state
fresh specimen