In vitro transcript Haloferax volcanii 16S rRNA
First, genomic DNA was isolated from H. volcanii (H26) using standard phenol-chloroform extraction. The DNA was used as template for the PCR amplification of the 16S region using primers containing sites necessary for T7-based in vitro transcription (fwd: TAATACGACTCACTATAGGGATTCCGGTTGATCCTGCCGGA, rev: AGGAGGTGATCCAGCCGCAGA). The T7 RiboMax express kit (Promega) was used for the in vitro production of 16S rRNA under standard conditions.
ENA-FIRST-PUBLIC
2022-11-14
ENA-LAST-UPDATE
2022-11-14
External Id
SAMEA111560022
INSDC center alias
Microbiology_UR
INSDC center name
microbiology_ur
INSDC first public
2022-11-14T15:53:25Z
INSDC last update
2022-11-14T15:53:25Z
INSDC status
public
Submitter Id
hvo_ivt
sample name
hvo_ivt
strain
PCR template origin H26