Sample 2
Protocols: Human glioblastoma cells (U87MG) were obtained from the American Type Culture Collection (Manassas, Va.). Cells were cultured in Dulbeccos Modified Eagle Medium supplemented with 10% fetal bovine serum (Gibco Fetal Bovine Serum South America, Thermo Scientific Fisher-US), 2mM L-glutamine, 50 U/ml penicillin, 50 ?g/ml streptomycin, at 37o C, 5% CO2 and 95% of humidity U87MG glioblastoma cells have been transfected, in biological triplicate, with a siRNA targeting praja2 gene or control siRNA. Cells were collected and homogenized in Trizol Reagent. Total RNA extraction was performed with a RNA Clean and Concentrator Kit (Zymo Research Corp., Irvine, CA, USA), following the manufacturers instructions. RNA purity and integrity were assessed with a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and a 4200 TapeStation instrument (Agilent Technologies, Santa Clara, CA, USA), respectively. For RNA purity, an A260/280 ratio of ?2.0 and a A260/230 ratio of 2.0-2.2 were considered acceptable; for RNA integrity, an RNA Integrity Number (RIN) of 9.0-10.0 has been obtained for all samples, indicating the absence of degradation and high integrity of RNA samples. For a precise estimation of the RNA concentration, a Qubit 2.0 fluorometer assay (Thermo Fisher Scientific, Waltham, MA, USA) has been employed
ENA-FIRST-PUBLIC
2022-06-29
ENA-LAST-UPDATE
2022-06-29
External Id
SAMEA10838354
INSDC center alias
Laboratory of Molecular Medicine and Genomics
INSDC center name
Laboratory of Molecular Medicine and Genomics
INSDC first public
2022-06-29T00:16:22Z
INSDC last update
2022-06-29T00:16:22Z
INSDC status
public
Submitter Id
E-MTAB-11137:Sample 2
broker name
ArrayExpress
cell_line
U-87 MG
common name
human
disease
glioblastoma multiforme
genotype
---
isolate
not applicable
organism part
brain
sample name
E-MTAB-11137:Sample 2