Sample 8
Protocols: Mice were sacrificed with a mixture of ketamine (300mg/kg, Narketan®, Vétoquinol Biowet, Poland) and xylazine (30mg/kg, VetaXyl®, Vet-Agro, Poland). Lumbar spinal cord were collected and snap‐frozen in liquid nitrogen and stored at −80°C for further analysis. Total RNA was extracted from lumbar spinal cord neuromere harvested from 32-weeks-old mice. RNA was isolated using RNeasy® Plus Universal Mini Kit and QIAzol® Lysis Reagent (both Qiagen, USA), according to manufacturer's recommendations. Subsequently, RNA integrity (RIN), quality and quantity were evaluated with microcapillary electrophoresis (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). Only samples with RIN above 8 were used for further analysis. For library construction, DNA/RNA is extracted from a sample. After performing quality control (QC), qualified samples proceed to library construction. The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \tagmentation\ combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
ENA-FIRST-PUBLIC
2022-10-16
ENA-LAST-UPDATE
2022-10-16
External Id
SAMEA111437447
INSDC center alias
Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury in Olsztyn
INSDC center name
Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury in Olsztyn
INSDC first public
2022-10-16T00:33:17Z
INSDC last update
2022-10-16T00:33:17Z
INSDC status
public
Submitter Id
E-MTAB-12252:Sample 8
age
32
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
disease
normal
genotype
wild type genotype
individual
70
organism part
lumbar spinal cord
sample name
E-MTAB-12252:Sample 8
sex
male
strain
C57BL/6
treatment
PBS