Target gene: ZmKAK1
Targeted mutagenesis of gene Zm00001e008508 (GeneID v5). Target sequence: ATGATGAAGATTCAGGGTTCGGG. Forward primer: AGATAAGGGTAAGGAGCAGCAG. Reverse primer: CGATGGCAGTATATCGTCCAGG. Expected size: 217bp.
ENA-FIRST-PUBLIC
2022-11-14
ENA-LAST-UPDATE
2022-11-14
External Id
SAMEA111385073
INSDC center alias
ENS Lyon
INSDC center name
ENS Lyon
INSDC first public
2022-11-14T15:53:09Z
INSDC last update
2022-11-14T15:53:09Z
INSDC status
public
Submitter Id
NP16
age
Protoplasts isolated from maize leaf 2 weeks after sowing
cell_type
Leaf protoplast
common name
maize
cultivar
Maize inbred line A188
dev_stage
48h after transfection
genetic modification
Transfection with CRISPR/Cas9 constructs
genotype
Maize inbred line A188
growth condition
Protoplasts in W5 medium, at 26�C, in the dark
individual
NP18
organism part
Leaf
ploidy
Diploid
protocol
Fierlej et al.
sample name
NP16
sampling time point
48h after transfection
sex
hermaphrodite