P4_0
Protocols: Primary airway epithelial cells were collected by nasal brushing. Collected cells were expanded for 1-2 passages in monolayer culture using Pneumacult Ex-Plus medium. After transfer to Transwell filters cells were grown for 2 days in submersion. Cell differentiation was induced by change to Pneumacult ALI medium and introduction of ALI. Cells were grown up to 21 days in ALI setting and collected at indicated days using Tri-Reagent (Invitrogen). Total RNA was isolated using Tri-Reagent (Invitrogen). RNA isolates were DNase treated. Libraries were generated with Illumina TruSeq Stranded mRNA Library Prep Kit.
ENA first public
2021-11-29
ENA last update
2021-11-29
External Id
SAMEA11320701
INSDC center alias
Department of Systems Biology and Engineering, Silesian University of Technology
INSDC center name
Department of Systems Biology and Engineering, Silesian University of Technology
INSDC first public
2021-11-29T00:29:03Z
INSDC last update
2021-11-29T00:29:03Z
INSDC status
public
Submitter Id
E-MTAB-11209:P4_0
broker name
ArrayExpress
cell type
ciliated cell
common name
human
developmental stage
adult
disease
normal
growth condition
Pneumacult ALI differentiation media
individual
P4
organism part
airway epithelium
sample name
E-MTAB-11209:P4_0
sex
female