Wild Type - Rep 2
Protocols: Total RNA was isolated from wt and Ercc1-/- pancreata Total RNA isolation kit (Qiagen) as described by the manufacturer For RNA-Seq, libraries were prepared using the Illumina® TruSeq® mRNA stranded sample preparation kit. Library preparation started with 1μg total RNA. After poly-A selection (using poly-T oligo-attached magnetic beads), mRNA was purified and fragmented using divalent cations under elevated temperature. The RNA fragments underwent reverse transcription using random primers. This is followed by second strand cDNA synthesis with DNA Polymerase I and RNase H. After end repair and A-tailing, indexing adapters were ligated. The products were then purified and amplified (14 PCR cycles) to create the final cDNA libraries. After library validation and quantification (Agilent 2100 Bioanalyzer), equimolar amounts of library were pooled. The pool was quantified by using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System.
ENA first public
2021-05-15
ENA last update
2021-05-15
External Id
SAMEA8651316
INSDC center alias
IMBB-FORTH
INSDC center name
IMBB-FORTH
INSDC first public
2021-05-15T00:06:56Z
INSDC last update
2021-05-15T00:06:56Z
INSDC status
public
Submitter Id
E-MTAB-10393:Wild Type - Rep 2
age
15
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
disease
normal
genotype
wild type genotype
organism part
pancreas
sample name
E-MTAB-10393:Wild Type - Rep 2
sex
male
strain
C57BL/6J