SARS-CoV-2: wastewater surveillance
collection_date
2021-10-25
geo_loc_name
USA: Mississippi
isolation_source
wastewater
collection_site_id
PAS
ww_population
34768
ww_sample_duration
0
ww_sample_matrix
raw wastewater
ww_sample_type
grab
collection_volume
45L
ww_surv_target_1
SARS-CoV-2
ww_surv_target_1_known_present
yes
collected_by
FDA Center for Food Safety and Applied Nutrition
purpose_of_ww_sampling
public health surveillance community-level
ww_flow
20592640
ww_industrial_effluent_percent
11
ww_ph
6.8
ww_population_source
utilities billing records
ww_pre_treatment
2 inch bar screener
ww_sample_site
wastewater treatment plant
ww_temperature
26
ww_total_suspended_solids
114
purpose_of_ww_sequencing
Pilot Exercise
sequenced_by
FDA Center for Food Safety and Applied Nutrition
ww_endog_control_1
crAssphage
ww_endog_control_1_conc
Not provided
ww_endog_control_1_protocol
RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 (protocols.io)
ww_endog_control_1_units
Not provided
ww_endog_control_2
Not provided
ww_endog_control_2_protocol
Not provided
ww_surv_target_1_extract
31.00, 31.16, 29.53
ww_surv_target_1_extract_unit
Ct values
ww_surv_target_1_gene
N gene (N1)
ww_surv_target_1_protocol
RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500
ww_surv_target_2
SARS-CoV-2
ww_surv_target_2_conc
30.72, 32.79, 32.97
ww_surv_target_2_conc_unit
copies (Ct value)
ww_surv_target_2_gene
N gene (N2)
ww_surv_target_2_known_present
yes
ww_surv_target_2_protocol
RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io)
concentration_method
peg precipitation + ultracentrifugation
extraction_control
Murine norovirus
extraction_method
qiagen rneasy kit
project_name
GenomeTrakr; LFFM ww project
ww_surv_target_1_conc
31.00, 31.16, 29.53
ww_surv_target_1_conc_unit
Ct value
Center Name
CFSAN
BioSampleModel
SARS-CoV-2: wastewater surveillance