Bottle2_Day224
Bottle 2; active microcosm containing the methanogenic benzene-degrading enrichment culture DGG-B, 224 days of incuabtion, amended with 0.1 mL air on Day 53, amended with 10 mL air on Days 94 and 224, bottle presentally aerobic, aerobic benzene degradation has resumed
collection_date
2018-10-29
depth
0.3 - 0.7 m
elev
300 m
env_broad_scale
Groundwater aquifer [ENVO:00012408]
env_local_scale
Petroleum-enriched sediments [ENVO:00002115]
env_medium
Sediment [ENVO:00002007]
geo_loc_name
USA: Oklahoma, oil refinery
lat_lon
36 N 97 W
isolation_source
Contaminated sediments from an oil refinery in Oklahoma, USA (Nales et al., 1998; Bioremediat J 2). The site was described as being from a saturated, highly anaerobic zone that had been chronically exposed to hydrocarbons.
oxy_stat_samp
aerobic
samp_collect_device
The cell pellet (10,000 x g centrifugation, 15 min) from 1 mL culture was frozen at -80 C for later DNA extraction using the Dneasy PowerSoil Kit (Qiagen) according to the manufacturer's procedure.
samp_store_dur
-80
temp
23 C
Benzene degradation activity
Aerobic
Total bacteria (16S rRNA gene copies/mL)
2.87E+08
Total archaea (16S rRNA gene copies/mL)
7.43E+06
ORM2 (16S rRNA gene copies/mL)
7.17E+05
OD1 (16S rRNA gene copies/mL)
2.48E+06
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.sediment