Bottle1_Day688
Bottle 1; active microcosm containing the methanogenic benzene-degrading enrichment culture DGG-B, 688 days of incuabtion, amended with 0.1 mL air on Day 39, amended with 10 mL air on Day 94, bottle presentally anaerobic, methanogenic benzene degradation continues
collection_date
2020-02-05
depth
0.3 - 0.7 m
elev
300 m
env_broad_scale
Groundwater aquifer [ENVO:00012408]
env_local_scale
Petroleum-enriched sediments [ENVO:00002115]
env_medium
Sediment [ENVO:00002007]
geo_loc_name
USA: Oklahoma, oil refinery
lat_lon
36 N 97 W
isolation_source
Contaminated sediments from an oil refinery in Oklahoma, USA (Nales et al., 1998; Bioremediat J 2). The site was described as being from a saturated, highly anaerobic zone that had been chronically exposed to hydrocarbons.
oxy_stat_samp
anaerobic
samp_collect_device
The cell pellet (10,000 x g centrifugation, 15 min) from 1 mL culture was frozen at -80 C for later DNA extraction using the Dneasy PowerSoil Kit (Qiagen) according to the manufacturer's procedure.
samp_store_dur
-80
temp
23 C
Benzene degradation activity
Methanogenic
Total bacteria (16S rRNA gene copies/mL)
3.91E+08
Total archaea (16S rRNA gene copies/mL)
1.70E+07
ORM2 (16S rRNA gene copies/mL)
1.89E+08
OD1 (16S rRNA gene copies/mL)
3.33E+06
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.sediment