Colonies_tetracycline_5 [C5]
source_name
plasmid DNA
strain
UCC2003
treatment
plasmid DNA transformed into B. breve UCC2003, plated on agar plates supplemented with 5 mg/L tetracycline, grown for 48 hours. Plasmid DNA then extracted from pooled colonies.
dilution
none
molecule subtype
plasmid DNA amplified with PCR