Outgrowth_Overnight_Nonselective_1_10_Dilution [R1-v2]
source_name
plasmid DNA
strain
UCC2003
treatment
plasmid DNA transformed into B. breve UCC2003, 20 mg/L, recoveries grown overnight. 20 mg/L Tetracycline then added to transformation recoveries in 1:10 dilution cultures that were grown for 48 hours. Plasmid DNA was then extracted from cultures.
dilution
1:10
molecule subtype
plasmid DNA amplified with PCR