single cell suspension positively enriched for macrophages using F4/80 magnetic beads from a spleen of a wild-type mouse
source_name
spleen
strain
homozygous for Hbatm1Paz, heterozygous for Hbbtm1Tow, hemizygous for Tg(HBA-HBBs)41Paz (-/-, +/-, Tg/0, non-sickling)= control hetero
tissue
spleen
enrichment
single-cell suspensions from spleen positively enriched for macrophages using anti-F4/80 antibody (rat, Bioscience)-coated Dynabeads (anti-rat IgG, Invitrogen)