host
Homo sapiens
isolation_source
stool
collection_date
2012-08/2015-12
geo_loc_name
Peru: Cusco
lat_lon
13.518840 S 71.975040 W
samp_mat_process
Metagenomic DNA from 10 random samples were pooled together to construct a functional metagenomic library. The pooled metagenomic DNA was cloned into an expression vector and transformed into E. coli indicator hosts. These E. coli transformant libraries were then screened against an antibiotic at a selective concentration. Resistant clones were collected from solid media and were sequenced using Nextera protocol on Illumina NextSeq500.
SeqRunID
P09SR5_ATM
FMG_library_id
P09
Selection_id
P09_ATM
antibiotic_name
Aztreonam
MIC (ug/ml)
8
colony_count
100
Avg_insert_size (bp)
2390.545
Library_size (GB)
11.01-11.49
Abx_class
Beta_lactam
BioSampleModel
Metagenome or environmental