ChIPseq input replicate 5
IP_input
input
replicate
1
breed
Canton
age
2-16h
sex
pooled male and female
tissue
whole embryo
genotype
WT
growth_protocol
Fly stocks were maintained with standard protocols. Embryos were collected from food plates (corn+yeast extract+sugar+agar) at 25C and used directly for experiments.
treatment
Embryos were cross linked in 1.8% formaldehyde, chromatin was sonicated to an average size of ∼500 bp (whole cell extract (WCE)), and 300 μl WCE from ∼120 mg embryos was incubated with protein A–conjugated Dynabeads (Invitrogen 100-02D), coated with antibodies against D. melanogaster Chn, Med-19 (Custom antibodies raised the lab) and histones marks (H3K27Ac, H3K3me1 and H3K4me3). For input controls, 50 μl WCE was used. Preparations of DNA libraries for single-end sequencing were done according to instructions from Illumina.
BioSampleModel
Model organism or animal