Anopheles gambiae ASEMBO sex chromosome evolution
Anopheles rDNA are located on the sex chromosomes, often exclusively on the X chromosome, as for the Anopheles gambiae G3 and Anopheles arabiensis Dongola strains. This exclusively X-linked rDNA locus was used to develop a transgenic An. Gambiae G3 strain (?2-Ppo1) that expresses the I-Ppo1 endonuclease during male meiosis, which targets and cleaves An. gambiae 28S rDNA (Galizi et al., 2014). The rDNA locus of An. gambiae ASEMBO strain is located in both the X- and Y-chromosomes. To test whether recombination can occur between the rDNA loci of the An. gambiae sex chromosomes, Introgression of the An. gambiae YASEMBO into the transgenic meiotic rDNA-cleaving An. gambiae ?2-Ppo1 G3 strain was perfrmed. Backcrossing of the transgenic male offspring over multiple generations was conducted to selectively cleave the YASEMBO rDNA, while uncleaved XG3 chromosome was introduced every generation through backcrossing of wildtype An. gambiae G3 females. Sequencing was then conducted to assess whether after 24 generations of rDNA cleavage, this had resulted in repair, deletions, or other mutations of rDNA copies.
ENA-FIRST-PUBLIC
2022-08-05
ENA-LAST-UPDATE
2022-08-05
External Id
SAMEA110375442
INSDC center alias
Infravec
INSDC center name
Infravec
INSDC first public
2022-08-05T20:30:25Z
INSDC last update
2022-08-05T20:30:25Z
INSDC status
public
Submitter Id
TNA5066_G3_wt_male
common name
African malaria mosquito
infravec request number
5066
sample name
TNA5066_G3_wt_male
study accession
PRJEB35275
strain
PEST