FCA_P45_female_oenocyte_L17
Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols
ENA first public
2021-07-05
ENA last update
2021-07-05
External Id
SAMEA9296286
INSDC center alias
BCM
INSDC center name
Baylor College of Medicine
INSDC first public
2021-07-05T16:25:14Z
INSDC last update
2021-07-05T16:25:14Z
INSDC status
public
Submitter Id
E-MTAB-10628:FCA_P45_female_oenocyte_L17
age
5
broker name
ArrayExpress
cell type
oenocyte
common name
fruit fly
developmental stage
adult
genotype
PromE800-GAL4; UAS-unc84GFP
organism part
abdominal segment element
post analysis single cell quality
pass
sample name
E-MTAB-10628:FCA_P45_female_oenocyte_L17
sex
female
single cell identifier
FCA_P45_female_oenocyte_L17
single cell well quality
OK
strain
w1118