Human_colon_16S8157897
Protocols: For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol.
ENA first public
2021-08-19
ENA last update
2021-08-19
External Id
SAMEA9697331
INSDC center alias
Wellcome Sanger Institute
INSDC center name
Wellcome Sanger Institute
INSDC first public
2021-08-19T12:17:04Z
INSDC last update
2021-08-19T12:17:04Z
INSDC status
public
Submitter Id
E-MTAB-9532_2:Human_colon_16S8157897
age
60 to 65
broker name
ArrayExpress
cell type
B cell
common name
human
developmental stage
adult
individual
432c
organism part
veniform appendix
sample name
E-MTAB-9532_2:Human_colon_16S8157897
sex
female