Hom2
Protocols: Euthanize mice using carbon dioxide (CO2) for 1-2 min or until breathing stops. Next, perform cervical dislocation on each mouse.Extract the mouse testis tissue and remove the capsule Total RNA was isolated using RNeasy mini kit (Qiagen, Germany). Strand-specific libraries were prepared using the TruSeq® Stranded Total RNA Sample Preparation kit (Illumina, USA)following the manufacturer's instructions. Briefly, ribosomal RNA was removed from total RNA using Ribo-Zero rRNA removal beads. Following purification, the mRNA is fragmented into small pieces using divalent cations under 94℃ for 8 min. The cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers. This is followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments then go through an end repair process, the addition of a single 'A' base, and then ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library. Purified libraries were quantified by Qubit® 2.0 Fluorometer (Life Technologies, USA) and validated byAgilent 2100 bioanalyzer (Agilent Technologies, USA) to confirm the insert size and calculate the mole concentration.
ENA-FIRST-PUBLIC
2022-08-17T00:11:19Z
ENA-LAST-UPDATE
2022-08-17T00:11:19Z
External Id
SAMEA7192470
INSDC center name
Nanjing Medical University
INSDC first public
2022-08-17T00:11:19Z
INSDC last update
2022-08-17T00:11:19Z
INSDC status
public
Submitter Id
E-MTAB-9486:Hom2
age
10
broker name
ArrayExpress
common name
house mouse
developmental stage
juvenile stage
genotype
Pnldc1 -/-
organism part
testis
sample name
E-MTAB-9486:Hom2
scientific_name
Mus musculus
sex
male
strain
C57BL/6J