Spm rep2
Protocols: Polyamine, flg22 and mock (water) treatments were performed in three biological replicates by leaf- infiltration of 5-week-old wild-type (Col-0) plants. Infiltrated leaves were collected and frozen in liquid nitrogen at 24 h of treatment for total RNA extraction. Seeds of the different genotypes were sown on a soil mixture containing peat moss (40%), vermiculite (50%) and perlite (10%), and stratified in the dark at 4°C for 2-3 days. Plants were grown at 20-22℃ under 12-hour light/ 12-hour dark photoperiod cycles. Leaf-infiltration with 1-ml needless syringe Total RNA was extracted using TriZol (Thermo Fisher) and further purified using RNeasy kit (Qiagen) according to manufacturer's instructions. Libraries were prepared using the MGIEasy RNA Library Prep kit (MGI Tech) according to manufacturer's instructions
ENA-FIRST-PUBLIC
2022-10-21
ENA-LAST-UPDATE
2023-01-01
External Id
SAMEA110128813
INSDC center alias
Departament de Biologia, Sanitat i Medi Ambient. Facultat de Farmacia i Ciencies de l'Alimentacio. Seccio de Fisiologia Vegetal. Universitat de Barcelona. Barcelona, Spain
INSDC center name
Departament de Biologia, Sanitat i Medi Ambient. Facultat de Farmacia i Ciencies de l'Alimentacio. Seccio de Fisiologia Vegetal. Universitat de Barcelona. Barcelona, Spain
INSDC first public
2022-10-21T07:00:03Z
INSDC last update
2023-01-01T00:28:10Z
INSDC status
public
Submitter Id
E-MTAB-11820:Spm rep2
age
5
broker name
ArrayExpress
common name
thale cress
developmental stage
sporophyte vegetative stage
genotype
wild type genotype
organism part
rosette leaf
sample name
E-MTAB-11820:Spm rep2
ecotype
Col-0