Hnf4a_ATAC-seq_CreNeg_veh_rep1
Protocols: Mice were treated acutely with an intraperitoneal injection of dexamethasone or vehicle, and liver tissue harvested one hour later. For wild type samples ATAC-seq was performed on flash frozen liver tissue employing Active Motif\u2019s end-to-end ATAC-seq service (https://www.activemotif.com/catalog/1233/atac-seq-service). Otherwise, ATAC-seq on flash-frozen liver tissue was performed using the Active Motif ATAC-seq kit, first isolating nuclei using the OMNI-ATAC protocol (Corces et al., 2017; https://doi.org/10.1038/nmeth.4396), subsequently following manufacturer\u2019s instructions for the kit. For wild type samples ATAC-seq was performed on flash frozen liver tissue employing Active Motif\u2019s end-to-end ATAC-seq service (https://www.activemotif.com/catalog/1233/atac-seq-service). Otherwise, ATAC-seq on flash-frozen liver tissue was performed using the Active Motif ATAC-seq kit, first isolating nuclei using the OMNI-ATAC protocol (Corces et al., 2017; https://doi.org/10.1038/nmeth.4396), subsequently following manufacturer\u2019s instructions for the kit.
ENA first public
2022-01-19
ENA last update
2022-01-19
External Id
SAMEA12363440
INSDC center alias
University of Manchester
INSDC center name
University of Manchester
INSDC first public
2022-01-19T00:26:24Z
INSDC last update
2022-01-19T00:26:24Z
INSDC status
public
Submitter Id
E-MTAB-102661642535014:Hnf4a_ATAC-seq_CreNeg_veh_rep1
age
10 to 12
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
Hnf4aFlox AlbCre-/-
individual
Hnf4a_ATAC-seq_CreNeg_veh_rep1
organism part
liver
sample name
E-MTAB-102661642535014:Hnf4a_ATAC-seq_CreNeg_veh_rep1
sex
male
strain
C57BL/6