KO_gut_scRNAseq
strain
C57BL6
isolate
For gut tissues, they were washed, cut into 1-1.5 cm sections, incubated with Hanks' Balanced Salt Solution (HBSS,) containing 5mM EDTA and 1mM DTT at 37C for 20 mins to remove epithelial cells, then minced and dissociated in RPMI containing 10% FBS, liberase TL (1 mg/ml; Roche) and DNase1 (100 g/ml; Roche) with constant stirring at 37C for 30 min.
dev_stage
6-8 weeks old
sex
female
tissue
Gut
collection_date
missing
geo_loc_name
missing
BioSampleModel
Model organism or animal