Homo sapiens
Protocols: Palatal connective tissue grafts (CTGs) from four groups 1) anterior deep (AD), 2) anterior superficial (AS), 3) posterior deep (PD), and 4) posterior superficial (PS) were obtained from 16 systemically and periodontally healthy subjects per group. The Ethics Committee, Bern, Switzerland (ethical code ID 2018-00661 from 13.08.2018) approved the study, and informed consent was obtained from all patients. The CTGs were biopsied during recession coverage surgeries performed by the same periodontist (Prof. Dr. Sculean). CTGs harvested in the region of lateral incisor, canine and premolars are designated as anterior (A), whereas CTGs harvested in the molars' area are designated as posterior (P). At each location, the tissue sample was harvested in toto as a free gingival graft (~6x4 mm). Subsequently, the epithelial layer of ~0.5 mm was removed by sharp dissection using a C15 blade and the CTG was split into two thinner grafts – a superficial (S) located immediately subepithelially and a deep (D) located closer to the bone. Immediately after the excision, ~1/3 of each tissue sample was immediately placed in RNAlater (ThermoFisher Scientific, Basel, Switzerland) for 20 h before tissue homogenization using gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) followed by RNA extraction using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Basel, Switzerland). Quantity and quality of RNA extracted from each of the 64 collected CTGs was assessed using the Qubit RNA BR Assay Kit on a Qubit 4.0 fluorometer (ThermoFisher Scientific) as well as the Fragment Analyzer RNA Kit on an Advanced Analytical Fragment Analyzer System (Agilent Technologies, Basel, Switzerland). Sequencing libraries were produced using an illumina TruSeq Stranded mRNA Library Prep kit in combination with TruSeq RNA UD Indexes according to illumina's guidelines (Illumina, San Diego, CA, USA).
ENA-FIRST-PUBLIC
2023-11-16T17:00:05Z
ENA-LAST-UPDATE
2023-11-16T17:00:05Z
External Id
SAMEA114003498
INSDC center name
University of Bern, Interfaculty Bioinformatics Unit
INSDC first public
2023-11-16T17:00:05Z
INSDC last update
2023-11-16T17:00:05Z
INSDC status
public
Submitter Id
E-MTAB-13141:ad5c
age
over 18
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
palate
sample name
E-MTAB-13141:ad5c
sampling site
anterior deep palate
scientific_name
Homo sapiens
sex
not available