Homo sapiens
Protocols: To profile the transcriptome within the ccRCC TME, we performed ST-seq on ten fresh frozen tissue sections collected in the operating theatre from six patients with ccRCC (n = 2 para-tumour, n = 4 low-grade and n = 4 high-grade) presenting at a tertiary referral hospital from June 2021 to January 2022 (Fig. 1A). Additional clinical characteristics of the ccRCC patients at presentation include patient LG_2 with a vena cava thrombus (VCT); patient HG_1 with pleural metastases; and patient HG_3 with a third recurrent lesion (Table 1). Furthermore, for patients HG_1 and HG_3, para-tumour (pTME) tissue was collected from adjacent surgically normal margin. All six patients provided informed consent and this study received ethics approval from Metro South Human Research Ethics Committee (Reference Number HREC/16/QPAH/353 and HREC/12/QPAH/125). ST libraries were prepared by Visium Spatial Gene Expression Reagent Kits ST libraries were prepared by Visium Spatial Gene Expression Reagent Kits
ENA-FIRST-PUBLIC
2023-12-01T00:42:24Z
ENA-LAST-UPDATE
2023-12-01T00:42:24Z
External Id
SAMEA112772780
INSDC center name
QCIF Facility for Advanced Bioinformatics, Institute for Molecular Bioscience, The University of Queensland, Australia
INSDC first public
2023-12-01T00:42:24Z
INSDC last update
2023-12-01T00:42:24Z
INSDC status
public
Submitter Id
E-MTAB-12767:HG_3.2
age
57
broker name
ArrayExpress
cell_type
spatial
clinical history
obese, non diabetic, ex-smoker
clinical information
2008 Right nephrectomy. 2017 Partial left nephrectomy. 2021 Redo partial left nephrectomy
common name
human
developmental stage
adult
disease
renal cell carcinoma
disease staging
G3; pT1a
individual
HG 3
isolate
not applicable
organism part
kidney
sample name
E-MTAB-12767:HG_3.2
scientific_name
Homo sapiens
sex
male
tumor size
18