Pseudomonas putida KT2440
Protocols: Biomass was harvested from mid-exponential cultures by means of centrifugation. Pseudomonas alloputida KT2440 was grown using MP medium to mid-exponential phase, using phenylethanol (5 mM) as carbon and energy source. Cultures were grown with different lanthanide elements (La, Nd, Er, a lanthanide cocktail [Ce, Nd, Dy, Ho, Er, Yb]; 50 nM) or without lanthanide addition. Total RNA was extracted with TRI reagent (Applied Biosystems), and mRNA was enriched using the RiboMinus bacteria 2.0 transcriptome isolation kit (Invitrogen). Sequencing libraries were prepared with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs).
ENA-FIRST-PUBLIC
2023-12-01T00:42:43Z
ENA-LAST-UPDATE
2023-12-01T00:42:43Z
External Id
SAMEA113907580
INSDC center name
Friedrich Schiller University Jena Institute of Biodiversity Aquatic Geomicrobiology Group
INSDC first public
2023-12-01T00:42:43Z
INSDC last update
2023-12-01T00:42:43Z
INSDC status
public
Submitter Id
E-MTAB-13102:None.2
broker name
ArrayExpress
collection date
not collected
genotype
wild type genotype
geographic location (country and/or sea)
not collected
growth condition
MP medium supplemented with 5 mM pheylethanol
isolate
not applicable
organism part
whole organism
replicate
2
sample name
E-MTAB-13102:None.2
scientific_name
Pseudomonas putida
strain
KT2440