Homo sapiens
Protocols: Human normal GC cell line GES1 and GC cancer cell line AGS were purchased from the Chinese Academy of Sciences cell bank (Shanghai, China). All cells were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA), penicillin (50 U/ml, Sangon, China), streptomycin (50 mg/ml, Sangon, China), at 37°C in a 5% CO2 humidified atmosphere. The lentiviral vector pLKO.1-puro-shRNA-CPSF6 was constructed for CPSF6 knockdown. 293T cells were transfected for the lentiviral production. After 48 h and 72 h, the supernatant was collected. Then AGS cells were infected by supernatant for 72 h. Finally, cells were maintained in the presence of puromycin (2mg/ml) for 1-2 weeks. Total RNA was extracted using TRIzol (Invitrogen, USA) according to the manufacturer's instructions. Briefly, 50 μg total RNA was incubated with M280 beads, which were bound by Bio-oligo dT (20). After cDNA synthesis, the 3′UTR fragments were released by Gsu I digestion and then subjected to deep sequencing.The libraries were constructed, using NEBNext® Ultra™ II DNA Library Prep Kit and following manufacturer's instructions.
ENA first public
2021-07-31
ENA last update
2021-07-31
External Id
SAMEA7765451
INSDC center alias
School of Biomedical Engineering, Shanghai Jiao Tong University
INSDC center name
School of Biomedical Engineering, Shanghai Jiao Tong University
INSDC first public
2021-07-31T00:11:03Z
INSDC last update
2021-07-31T00:11:03Z
INSDC status
public
Submitter Id
E-MTAB-9980:AGS_3T-seq_shCtrl
broker name
ArrayExpress
cell line
AGS
cell type
epithelial cell
common name
human
developmental stage
adult
disease
gastric adenocarcinoma
organism part
stomach
rna interference
blank vector
sample name
E-MTAB-9980:AGS_3T-seq_shCtrl