root metagenome
For the analyses of microbiota abundances, tomato seeds were germinated at 22C during 1 week in sterile vermiculite. Afterwards, seedlings were transferred to pots (1 per pot) filled with 3 liter of natural soil obtained from coordinates 4024'21.6"N 349'58.1"W that were mixed with 1 part of clean river sand. These plants were cultivated for 3 weeks at three different temperatures 22C (22C shoot and root; 22SR), 36C (36C in root and shoot, 36SR) and 36TGRZ (36C in shoot and gradient from 36 to 20C in root) and irrigated with 150 ml of sterile water twice a week. We took 6 samples of the initial soil and maintained them at -20C. After 3 weeks, we took 6 samples of soil corresponding to rhizosphere and 6 samples of roots that were extensively washed to eliminate soil/microorganism traces) and freeze to -20C. Soil and roots were taken from the same area in the pot, corresponding to an intermedium part of the root system.
host
tomato
isolation_source
roots
collection_date
2019
geo_loc_name
Spain: Madrid,Pozuelo de Alarcon
lat_lon
40.24 N 3.49 W
strain
NA
isolate
a51
cultivar
NA
ecotype
NA
env_broad_scale
oaks land
env_local_scale
forest
env_medium
clay
isol_growth_condt
clay was mixed with 1 part of clean river sand. These plants were cultivated for 3 weeks at three different temperatures 22C (22C shoot and root; 22SR), 36C (36C in root and shoot, 36SR) and 36TGRZ (36C in shoot and gradient from 36 to 20C in root) and irrigated with 150 ml of sterile water twice a week.
air_temp_regm
24C
watering_regm
irrigated with 150 ml of sterile water twice a week.
BioSampleModel
Metagenome or environmental