Homo sapiens
Protocols: Briefly, 3 ml plasma per sample was diluted with 3 ml of 1X PBS (pH 7.5) followed by centrifugation at 5,000 x g for 3 min. Next, 3.5 ml of the supernatant was filtered with a nitrocellulose filter (0.8 µm pore size, VWR, Radnor, PA, USA). The extracellular vesicles were extracted by ExoEasy Serum Plasma Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol. The exosomal RNA were extracted by exoRNeasy Serum Plasma Kit (Qiagen) Libraries were prepared using QIAseq miRNAseq library kit following the manufacturer's instructions.
ENA first public
2021-09-01
ENA last update
2021-09-01
External Id
SAMEA8742686
INSDC center alias
Steno Diabetes Center Copenhagen
INSDC center name
Steno Diabetes Center Copenhagen
INSDC first public
2021-09-01T00:38:05Z
INSDC last update
2021-09-01T00:38:05Z
INSDC status
public
Submitter Id
E-MTAB-10458:AM-G11_S11
age
38
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
type I diabetes mellitus
fraction
extracellular vesicular exosome
individual
G11_S11
organism part
blood plasma
sample name
E-MTAB-10458:AM-G11_S11
sex
female