Mus musculus
Protocols: The mice were fasted overnight (11 hours) prior to liver isolation, and livers were kept at -80°C until analysis. C57Bl/6JRj mice (8 weeks of age) received either a long acting glucagon analogue (NNC9204-0043; terminal half-life of 5-6 hours, 1.5 nmol/kg twice daily) or PBS+1% BSA (twice daily). Drugs were administered over a period of 8 weeks, by subcutaneous injection. The initial dose of the glucagon analogue of 3 nmol/kg was reduced to 1.5 nmol/kg after 16 days of treatment, due to a weight decrease of >20% of one of the mice (the mouse was terminated and excluded from the study). Raw sequencing data were processed using bcl2fastq software version 2.20.0 (Illumina). Total DNA/RNA was purified by an AllPrep DNA/RNA Minikit (catalog no. 80204; QIAgen, Hilden, Germany) according to manufacturer's instructions and quality tested using a 2100 Bioanalyzer instrument (Agilent Genomics, Santa Clara, California, USA). The extracted RNA was DNase treated with RNeasy Minikit (catalog no. 74106; QIAgen, Hilden, Germany) and RNase-Free DNase set (catalog no. 79254; QIAgen, Hilden, Germany) according to manufacturer's protocol. All the above-mentioned processes were performed on a QIACube machine (QIAgen, Hilden, Germany). Library preparation was done using TruSeq Stranded Total RNA Library Prep Gold (catalog no. 20020599; Illumina, San Diego, California, USA) and IDT for Illumina-TruSeq RNA UD Indexes (cat 20022371; Illumina, San Diego, California, USA) according to the manufacturer's instructions
ENA-FIRST-PUBLIC
2023-12-01T00:42:18Z
ENA-LAST-UPDATE
2023-12-01T00:42:18Z
External Id
SAMEA112328717
INSDC center name
Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
INSDC first public
2023-12-01T00:42:18Z
INSDC last update
2023-12-01T00:42:18Z
INSDC status
public
Submitter Id
E-MTAB-12520:Sample 9
age
16
broker name
ArrayExpress
common name
house mouse
disease
normal
genotype
wild type genotype
isolate
not applicable
organism part
kidney
sample name
E-MTAB-12520:Sample 9
scientific_name
Mus musculus
sex
female
strain
C57BL/6JRj