Chamaelirium japonicum
accession name
Koshiki
air temperature regimen
26/21 degree Celsius
biological material altitude
112 m
biological material geographic location
Germany
biological material id
2889857_Kioshiki_Pfl1
biological material latitude
51,816000
biological material longitude
11,283000
biological material ploidy
diploid
checklist
BSDC00001
collection date
not collected
genus
Chionographis
geographic location (country and/or sea)
Japan
growth facility
root culture
library type
Hi-C
light regimen
16h light/8h dark
material source geographic location
Japan
plant anatomical entity
root
project name
Chionographis japonica Sequencing
sample description
In vitro root cultures of C. japonica were induced from leaf petioles. After gentle washing with water, petioles were surface sterilized with a diluted sodium hypochlorite solution (3% active chlorine) supplemented with two drops of Tween 20 for 15 min, followed by a four-times rinse in autoclaved distilled 1 water. Afterwards, petioles were cut into 3 mm segments under sterile conditions and cultivated on 1/2 Macro Murashige/Skoog (1/2 MS) medium (MURASHIGE AND SKOOG 1962) supplemented with 10.74 mikroM NAA, 0.44 mikroM BAP, 3% sucrose, and 0.8% Micro Agar, pH 5.8, in Petri dishes. The parameter in the growth chamber was 16 h light exposure at 26 degree C followed by 8 h darkness at 21 degree C. To avoid light stress, the explants were shadowed with paper sheets for two weeks. Three weeks later, the petiole segments were transferred to fresh medium. After additional six weeks, roots formed on the petiole segments were separated, multiplied, and subcultured on 1/2 MS medium supplemented with 2.69 mikrogramm NAA, 20% sucrose, 1g/l peptone, 230 mg/l NaH2PO4 x 2 H2O and 2.5 g/l Phytagel, pH 5.2, under the same growing conditions. Root tuffs were subcultured on fresh medium every 4 - 6 weeks.
sample id
IPK_LIMS:2889857
sample name
2889857_Kioshiki_Pfl1
species
japonica