Klebsiella pneumoniae
Protocols: Bacteria were grown in LB (Luria Bertani) medium and in DMEM (Dulbecco's Modified Eagle Medium) to exponential phase and cells were harvested at OD600 = 0.5. Cells were peleted by centrifugation, resuspended in PBS and subjected to UV crosslinking. Then bacteria were re-collected by centrifugation and flash frozen in liquid nitrogen Following mechanical lysis of the cells, Hfq with its bound RNAs was coimmunoprecipitated using anti-Flag antibody. Exposed regions of the RNAs were trimmed by RNases, the 50 and 30 ends of the RNAs were treated with T4 PNK, neighboring RNAs were ligated, the Hfq protein was digested, and RNA was then isolated using TRI-reagent RNAtag-seq with mudifications detailed in Melamed et al, Nature Protocols 2018
ENA-FIRST-PUBLIC
2023-12-01T00:42:53Z
ENA-LAST-UPDATE
2023-12-01T00:42:53Z
External Id
SAMEA114350469
INSDC center name
The Hebrew University of Jerusalem, Israel
INSDC first public
2023-12-01T00:42:53Z
INSDC last update
2023-12-01T00:42:53Z
INSDC status
public
Submitter Id
E-MTAB-13342:WT_2_LB
broker name
ArrayExpress
collection date
not collected
geographic location (country and/or sea)
not collected
growth condition
LB media
growth phase
exponential
isolate
not applicable
sample name
E-MTAB-13342:WT_2_LB
scientific_name
Klebsiella pneumoniae
strain
SGH10