Ovis aries
Protocols: After incubation, cells were rinsed in PBS twice before being lysed directly in Buffer RLT Plus from the RNeasy plus micro kit (Qiagen, Victoria, Australia). Cells were cultured in a humidified incubator at 37ºC, with 5% carbon dioxide. Growth medium provided contains 1 g/L D-glucose (Gibco, Victoria, Australia) Dulbecco's Modified Eagle Medium (DMEM), L-Glutamine and 110 mg/L Sodium pyruvate, supplemented with 10% foetal bovine serum (Bovogen, Victoria, Australia) and 1% Penicillin-Streptomycin (Gibco, Victoria, Australia). Total RNA was extracted from lysed cells using the RNeasy Plus Micro kit (Qiagen, Victoria, Australia). Quality of RNA was determined on the 2100 BioAnalyzer (Agilent Technologies, Victoria, Australia). All samples of at least an RNA Integrity Number (RIN) of ≥ 8.0 and an A260/280 ratio of 1.8 - 2.0 were accepted. Library preparation was performed on 1 µg total RNA per sample with the Illumina TruSeq® Stranded mRNA kit according to manufacturer's protocol (Illumina, San Diego, California).
ENA-FIRST-PUBLIC
2022-08-18
ENA-LAST-UPDATE
2022-08-18
External Id
SAMEA110668937
INSDC center alias
The University of Western Australia; Ear Science Institute Australia
INSDC center name
The University of Western Australia; Ear Science Institute Australia
INSDC first public
2022-08-18T04:15:53Z
INSDC last update
2022-08-18T04:15:53Z
INSDC status
public
Submitter Id
E-MTAB-12102:A13
broker name
ArrayExpress
cell_type
adipose tissue derived mesenchymal stem cell
common name
sheep
organism part
adipose tissue
replicate
5
sample name
E-MTAB-12102:A13