Homo sapiens
Protocols: AML cell lines were pre-treated with siRNA against S100A9 for 24 hours and were afterwards incubated with ABT-199 for an additional 24 hours. Total RNA was extracted and purified using the NucleoSpin RNA plus kit (Macherey–Nagel, Düren, Germany). Sample quality was checked by calculating the RNA integrity number (RIN value) using fragment analyser (Agilent), with 3 biological replicates from each group. The RNA sequencing (RNA-seq) library preparation was performed with 150 ng RNA using the Illumina KAPA RNA HyperPrep kit with RiboErase (HMR) (Illumina, Cambridge, UK).
ENA-FIRST-PUBLIC
2023-12-01T00:42:43Z
ENA-LAST-UPDATE
2023-12-01T00:42:43Z
External Id
SAMEA113904664
INSDC center name
Laboratory for Hematology and Immunology, Department of Biomedical Sciences, Vrije Universiteit Brussel, Brussels, Belgium
INSDC first public
2023-12-01T00:42:43Z
INSDC last update
2023-12-01T00:42:43Z
INSDC status
public
Submitter Id
E-MTAB-13095:Sample 22
broker name
ArrayExpress
cell_line
MOLM13
collection date
not collected
common name
human
developmental stage
adult
disease
acute myeloid leukemia
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blood
sample name
E-MTAB-13095:Sample 22
scientific_name
Homo sapiens