Escherichia coli str. K-12 substr. MG1655
Protocols: Samples were prepared according to the protocol found in this paper: doi:10.1038/nprot.2017.115 Wild-type and Hfq::3XFLAG E.coli were grown in Gutnick minimal media with 3mM NH4Cl as the sole nitrogen source, and cell samples were taken during exponential growth (N+), ~20min following N-runout and induction of growth arrest (N-), 24hr following N-runout and induction of growth arrest (N-24) and 2hr following addition of NH4Cl to N-24 cells (N-24+2). Samples were prepared according to the protocol found in this paper: doi:10.1038/nprot.2017.115. Final RNA purification by TRIzol extraction Preparation of strand specific cDNA performed by Vertis Biotechnologies. cDNA synthesis was conducted with oligonucleotide (dT)-adapter primers and M-MLV reverse transcriptase, followed by PCR amplification using TruSeq-designed primers from Illumina guidelines.
ENA-FIRST-PUBLIC
2023-12-01T00:42:57Z
ENA-LAST-UPDATE
2023-12-01T00:42:57Z
External Id
SAMEA114476171
INSDC center name
Department of Infectious Diseases, Imperial College London
INSDC first public
2023-12-01T00:42:57Z
INSDC last update
2023-12-01T00:42:57Z
INSDC status
public
Submitter Id
E-MTAB-13403:WT_N-_3
broker name
ArrayExpress
collection date
not collected
environmental stress
Nitrogen stress
genotype
wild type genotype
geographic location (country and/or sea)
not collected
growth condition
short-term starvation in Gutnick minimal media with 3mM NH4Cl (N+)
isolate
not applicable
sample name
E-MTAB-13403:WT_N-_3
scientific_name
Escherichia coli K-12
strain
K-12
substrain
MG1655