Mycobacterium tuberculosis H37Rv
Protocols: 109 bacterial isolates were collected from four hospitals in the Campania region (Southern Italy).Bacterial isolation was performed via routine methods using mycobacterial growth indicator tubes (Bactec MGIT 960; Becton Dickinson, Sparks, MD, US) and Lowenstein-Jensen agar slants. Genomic DNA was obtained from clinical strains using two column-based DNA extraction methods (QIAamp DNA minikit, Qiagen, Germany), according to the instructions. The purified DNA was qualitatively and quantitatively evaluated using NanoDrop and Qubit dsDNA analysis kits (ThermoFisher Scientific, Waltham, MA, USA). Indexed libraries were prepared starting from 60 ng of each DNA sample according to Illumina DNA Prep sample preparation kits (Illumina Inc., San Diego, CA, USA). Final libraries concentrations and size were assessed with Quant-IT DNA Assay Kit and Agilent 4200 Tapestation System (Agilent Technologies, Milan, Italy), respectively.
ENA-FIRST-PUBLIC
2023-11-17T00:47:27Z
ENA-LAST-UPDATE
2023-11-17T00:47:27Z
External Id
SAMEA114625892
INSDC center name
University of Salerno
INSDC first public
2023-11-17T00:47:27Z
INSDC last update
2023-11-17T00:47:27Z
INSDC status
public
Submitter Id
E-MTAB-13058_2:2019_CO_1_S37
broker name
ArrayExpress
collection date
not collected
geographic location (country and/or sea)
not collected
isolate
not applicable
lineage
lineage4
sample name
E-MTAB-13058_2:2019_CO_1_S37
scientific_name
Mycobacterium tuberculosis
strain
H37Rv
type-material
type strain of Mycobacterium tuberculosis