Homo sapiens
Protocols: Cells were detached by addition of trypsin. Cells suspensions were quenched with medium and centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction. Total RNA was extracted using RNeasy Mini Kit (QIAGEN). Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 1 ug (20 ng/μl) of high-quality total RNA (using Monarch Total RNA Miniprep Kit according to manufacturer's instructions, New England Biolabs, Hitchin, UK) was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-A enrichment. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.
ENA-FIRST-PUBLIC
2023-12-01T00:42:39Z
ENA-LAST-UPDATE
2023-12-01T00:42:39Z
External Id
SAMEA113547112
INSDC center name
College of Health, Medicine and Life Sciences, Division of Biosciences, Brunel University London
INSDC first public
2023-12-01T00:42:39Z
INSDC last update
2023-12-01T00:42:39Z
INSDC status
public
Submitter Id
E-MTAB-12978:NSG_3
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
normal
genotype
wild type genotype
organism part
salivary gland
sample name
E-MTAB-12978:NSG_3
scientific_name
Homo sapiens