Klebsiella pneumoniae
Protocols: SGH10 bacteria were grown under DMEM (Dulbecco's Modified Eagle Medium) and LB(Lysogeny broth). Bacteria were harvested by centrifugation. The bacterial pellet was resuspended in TE buffer, mixed with lysosyme (SigmaAldrich) in a final concentration of 0.9 mg/ml, and immediately frozen in liquid nitrogen. The frozen pellet was subjected to two cycles of thaw and freeze at 37°C and in liquid nitrogen, respectively, and RNA was extracted using Tri-Reagent (Sigma-Aldrich) RNAtag-seq with mudifications detailed in Melamed et al, Nature Protocols 2018
ENA-FIRST-PUBLIC
2023-12-01T00:42:53Z
ENA-LAST-UPDATE
2023-12-01T00:42:53Z
External Id
SAMEA114349258
INSDC center name
The Hebrew University of Jerusalem, Israel
INSDC first public
2023-12-01T00:42:53Z
INSDC last update
2023-12-01T00:42:53Z
INSDC status
public
Submitter Id
E-MTAB-13341:WT_2_DMEM
broker name
ArrayExpress
collection date
not collected
geographic location (country and/or sea)
not collected
growth condition
DMEM media
growth phase
exponential
isolate
not applicable
sample name
E-MTAB-13341:WT_2_DMEM
scientific_name
Klebsiella pneumoniae
strain
SGH10