Salmo salar
Protocols: RNA seq analysis was conducted on IFNα_ISAV/ IFNα_IPNV (n=4) treatment groups at 2 hours post-infection and results were compared with wild SHK-1 cells (n=4). For Knock out (KO) experiment, IFNα_ISAV/ IFNα_IPNV (n=4) treatment groups at 2 hours post-infection were used as control and below KO groups were used as treatment groups. The number of each group was mentioned in brackets. KO_CRFB1a_IFNα_ISAV_SHK-1 (n=3) KO_CRFB5a_IFNα_ISAV_SHK_1 (n=3) KO_I10R2_IFNα_ISAV_SHK-1 (n=2) KO_I10Rb_IFNα_ISAV_SHK-1 (n=3) KO_IFNGR2b_IFNα_ISAV_SHK-1 (n=3) KO_CRFB1a_IFNα_IPNV_SHK-1 (n=3) KO_CRFB5a_IFNα_IPNV_SHK_1 (n=3) KO_I10R2_IFNα_IPNV_SHK-1 (n=2) KO_IFNGR2b_IFNα_IPNV_SHK-1 (n=3) KO_I10Rb_IFNα_IPNV_SHK-1 (n=3) KO_All_IFNα_ISAV_SHK-1 (n=2) KO_All_IFNα_IPNV_SHK-1 (n=2) Total RNA was extracted from collected cells by using the Direct-zol RNA Microprep Kits (Zymo) using the manufacturer's protocol. The RNA purity was measured using a Tape station, and the RNA concentration was determined using spectrophotometry (Nanodrop ND1000). Novogene mRNA-seq Services did library preparation. Briefly, the library is prepared according to Salmo salar, and subsequently tested for its quality (Library QC).
ENA-FIRST-PUBLIC
2023-12-01T00:42:40Z
ENA-LAST-UPDATE
2023-12-01T00:42:40Z
External Id
SAMEA113601492
INSDC center name
PhD fellow, Norwegian University of Life science
INSDC first public
2023-12-01T00:42:40Z
INSDC last update
2023-12-01T00:42:40Z
INSDC status
public
Submitter Id
E-MTAB-13068:KO_I10Rb_IFNα_IPNV_SHK-1
broker name
ArrayExpress
cell_line
SHK-1
collection date
not collected
common name
Atlantic salmon
genotype
IFNGR2b knockout
geographic location (country and/or sea)
not collected
infect
infectious pancreatic necrosis virus
isolate
not applicable
organism part
head kidney
sample name
E-MTAB-13068:KO_I10Rb_IFNα_IPNV_SHK-1
scientific_name
Salmo salar
stimulus
IFNα