Homo sapiens
Protocols: 1M PBMC were cultured in 200 μl medium in non-tissue culture treated 96-well round bottom plates (Corning) and left unstimulated or treated with 200 ng/ml LPS (Enzo Bioscience) or anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) for 16 h before being prepared for sequencing. After cell culture, cells were harvested and washed in PBS with 0.1% BSA. 20,000 cells from each condition were put into a channel of Chromium Next GEM Chip K of 10x Genomics platform. 10x 5' v2 10x 5' v2
ENA-FIRST-PUBLIC
2023-12-01T00:42:37Z
ENA-LAST-UPDATE
2023-12-01T00:42:37Z
External Id
SAMEA113446113
INSDC center name
Botnar Research Centre Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences University of Oxford
INSDC first public
2023-12-01T00:42:37Z
INSDC last update
2023-12-01T00:42:37Z
INSDC status
public
Submitter Id
E-MTAB-12805:PBMCs_Unsti_AS1830_GEX
age
36
broker name
ArrayExpress
cell_type
mononuclear cell
common name
human
developmental stage
adult
disease
ankylosing spondylitis
genotype
wild type genotype
individual
AS1830
organism part
blood
sample name
E-MTAB-12805:PBMCs_Unsti_AS1830_GEX
scientific_name
Homo sapiens
sex
female