Homo sapiens
isolate
Sequencing Library preparation was carried out in accordance with the manufacturer's instructions. In brief, total genomic DNA was extracted from tissue samples using the Amp Genomic DNA Kit (TIANGEN Biotech, Beijing, China). After shearing the genomic DNA to an average size of 200 bp, 2.5 ng of the fragmented DNA was used to create the sequencing library. End-repair and A-tailing was then perform, followed by the ligation of 1195-bp barcoded sequencing adaptors and treated DNA fragments. PCR was performed subsequently and the its product was purified using the magnetic beads.
age
NA
biomaterial_provider
Yifang Dai
sex
female
tissue
chorionic villi
BioSampleModel
Human