Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Protocols: Salmonella enterica subsp. enterica serovar Typhimurium SL1344 is a histidine auxotroph of parental strain ST4/74. Hoiseth,S.K. and Stocker,B.A.D. (1981) Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines. Nature, 291, 238-239. Cells were grown in liquid LB minimal medium at 37 degrees with shaking. Immunoprecipitations were done as described by Haycocks et al. (PLoS Pathog. 2015. 11:e1004605) using Vibrio cholerae strain E7946. After growing cells to midlog phase, crosslinking, and sonication, protein DNA complexes were immunoprecipitated using anti-FLAG or anti-Myc. Libraries were prepared using immunoprecipited protein-DNA complexes immobilised with Protein A-sepharose. DNA fragments were then blunt ended, A-tailed and barcoded. This was done using an NEB quick blunting and ligation kit, the Klenow fragment (5'-3' exo-, NEB) and NEXTflex ChIP-seq barcodes (Bioo Scientific). Following elution of complexes from the Protein-A sepharose, crosslinks were reversed and barcoded libraries were amplified by PCR. The number of PCR cycles was determined empirically for each library. After amplification, library concentration was quantified using Qubit analysis and real time PCR.
ENA-FIRST-PUBLIC
2023-12-01T00:42:22Z
ENA-LAST-UPDATE
2023-12-01T00:42:22Z
External Id
SAMEA112622028
INSDC center name
School of Biosciences University of Birmingham
INSDC first public
2023-12-01T00:42:22Z
INSDC last update
2023-12-01T00:42:22Z
INSDC status
public
Submitter Id
E-MTAB-12627:Rob N-terminal 3xFLAG tag sample 2
broker name
ArrayExpress
genotype
pAMNFrob
growth condition
mid exponential phase
isolate
not applicable
organism part
nucleoid
sample name
E-MTAB-12627:Rob N-terminal 3xFLAG tag sample 2
scientific_name
Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
serovar
Typhimurium
strain
SL1344