Ly6d specifically knock-down and fructose treatment
Mus musculus
Using lipofectamine RNAiMAX Transfection reagent (13778150, Invitrogen) and siRNA (Dharmacon, USA), the Ly6d gene was knocked down in vitro and cultured with Opti-MEM media according to the manufacturers instructions. Opti-MEM media was removed 6 h later, and the media was changed to complete DMEM/F12 media with fetal bovine serum, dexamethasone, and ITS containing  fructose.
strain
not provided
age
not provided
sex
not provided
tissue
Liver
cell_line
AML12
cell_type
Hepatocyte
disease
Normal
sample_type
Ly6d Knock-Down
treatment
fructose
replicate
biological replicate 2
BioSampleModel
Model organism or animal