CONV-RT_10Gy_Physioxia_4%O2_Rep2
Homo sapiens
source_name
HEK293T
cell line
HEK293T
genotype
WT
nuclease delivery
spCas9:RAG1B
transfection protocol
FuGENE; 48 hour culture post transfection
dose
10Gy
radiation facility
LINAC; Lausanne
treatment protocol
Trypsinize cells 24 hour post transfection at 4% O2, radiation exposure, replate cells for another 24 hours and then collect gDNA
target locus
RAG1 (spCas9:RAG1B gRNA)
illumina sequencing
Illumina MiSeq System; 250PE
figure displaying results for this library
Fig 3
number of raw reads used for normalization
214,700
pipeline used for the analysis
JoinT-seq