AD2941_CAL27_MMP10_sh70
Homo sapiens
Protocols: Each sample was collected by scrapping the cells from a 10cm cell culture dish. Pellet was stored at -20 degrees. AW13516, AW8507 and CAL27 cells were cultured in DMEM medium. The media was supplemented with 10% FBS, 2.5 mg/ml Amphotericin-B (Abbott) and 1.25 μl/ml Gentamycin (Abbott). The cells were cultured at 37 °C in a 5% CO2 incubator. RNA was extracted using the TRIzol solubilization and extraction protocol, as recommended by the manufacturer. Transcriptome sequencing libraries were constructed by using the TruSeq RNA library protocol (Illumina). Briefly, mRNA was purified from 4 μg of intact total RNA using oligodT beads and library preparation was done as per the manufacturer's instructions (TruSeq RNA Sample Preparation Kit, Illumina).
ENA-FIRST-PUBLIC
2023-11-23T00:24:38Z
ENA-LAST-UPDATE
2023-11-23T00:24:38Z
External Id
SAMEA11294746
INSDC center name
Advanced Centre for Treatment Research and Education in Cancer (ACTREC)
INSDC first public
2023-11-23T00:24:38Z
INSDC last update
2023-11-23T00:24:38Z
INSDC status
public
RNA interference
MMP10 shRNA
Submitter Id
E-MTAB-11185:AD2941_CAL27_MMP10_sh70
age
56
broker name
ArrayExpress
cell_line
CAL27
common name
human
developmental stage
adult
disease
tongue squamous cell carcinoma
ethnic group
European
genetic modification
none
genotype
wild type genotype
isolate
not applicable
organism part
tongue
sample name
E-MTAB-11185:AD2941_CAL27_MMP10_sh70
scientific_name
Homo sapiens
sex
male