MNase-seq of WT,10 min chase, replicate 1
Saccharomyces cerevisiae
Yeast cells were grown at 25C to OD600 of ~0.7. EdU was added to the culture at a final concentration of 130 M. At the same time, -factor was added to the culture at a final concentration of 50 ng/mL. After 5 minutes of EdU pulse, a sample was taken for nascent chromatin, after which cells were washed and resuspended in fresh media containing 130 M thymidine and 50 ng/mL -factor. Samples were taken at 10, 15, 20, and 40 minutes after the media change.
strain
WT
host
Not applicable
collection_date
2021-11-05
geo_loc_name
USA
sample_type
Cell culture
genotype
MATa, leu2-3,112, BAR1::TRP, can1-100, URA3::BrdU-Inc, ade2-1, his3-11,15
mating_type
a
temp
25
replicate
1
assay_type
MNase-seq
time_point
10 chase
BioSampleModel
Microbe, viral or environmental