Metagenome-assembled genome: ERR1278104_bin.43_CONCOCT_v1.1_MAG
uncultured Marinilabiliales bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278104 of study ERP014306.
ENA-FIRST-PUBLIC
2023-01-03
ENA-LAST-UPDATE
2023-01-03
External Id
SAMEA14080519
INSDC center alias
EBI
INSDC center name
European Bioinformatics Institute
INSDC first public
2023-01-03T00:32:53Z
INSDC last update
2023-01-03T00:32:53Z
INSDC status
public
Submitter Id
ERR1278104_bin.43_CONCOCT_v1.1_MAG
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
spadesv3.11.1
binning parameters
Default
binning software
CONCOCT v1.1
broker name
EMG broker account, EMBL-EBI
collection date
2011-10-01
completeness score
79.02
completeness software
CheckM
contamination score
2.47
environment (biome)
Host-associated
environment (feature)
Mammals
environment (material)
Digestive system
geographic location (country and/or sea)
Sweden
geographic location (latitude)
56.752724
geographic location (longitude)
16.688232
investigation type
metagenome-assembled genome
isolation_source
gut metagenome
metagenomic source
gut metagenome
project name
The moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.
sample derived from
SAMEA3878207
sample name
ERR1278104_bin.43_CONCOCT_v1.1_MAG
scientific_name
uncultured Marinilabiliales bacterium
sequencing method
Illumina HiSeq 2500
taxonomic identity marker
multi-marker approach