Human_colon_16S8117828
Homo sapiens
Protocols: For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. cDNA library preparation was were carried out according to the manufacturer's protocol.
ENA first public
2022-04-01
ENA last update
2022-04-01
External Id
SAMEA13608104
INSDC center alias
Wellcome Sanger Institute
INSDC center name
Wellcome Sanger Institute
INSDC first public
2022-04-01T00:17:49Z
INSDC last update
2022-04-01T00:17:49Z
INSDC status
public
Submitter Id
E-MTAB-9543_2:Human_colon_16S8117828
age
70 to 75
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
normal
genotype
wild type genotype
immunophenotype
Total
individual
386C
organism part
mesenteric lymph node
sample name
E-MTAB-9543_2:Human_colon_16S8117828
sex
female