Control3
Oryza sativa Japonica Group
Protocols: Anthers that include bicellular and tricellular stages of pollen were collected (11 to 13 stages of Anthers). Seeds of japonica rice cv. Dongjin was sterilized with 50% sodium hypochlorite and germinated in MS zero media in an incubator (14-h light/10-h dark, 28 °C (day)/22 °C (night), humidity 80%). After 1 week of germination, seedlings were transferred and grown in the greenhouse for 4 weeks under growth chamber conditions and transferred to the field. Mature anthers were frozen directly in Liquid nitrogen and finely ground using mortar. Grounded samples were homogenized using TRI reagents (RNAiso, Takara) and BCP (1-bromo-3-chloropropane). DNA, RNA, and protein layers were separated by centrifugation. Then, total RNA was extracted using the RNeasy Mini Kit (QIAGEN). The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \"tagmentation\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
ENA first public
2024-07-03
External Id
SAMEA114644351
INSDC center name
Kyung Hee University
INSDC last update
2023-11-20T21:41:05Z
INSDC status
public
Submitter Id
E-MTAB-13557:Control3
broker name
ArrayExpress
collection date
not collected
common name
Japanese rice
cultivar
Dongjin
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
anther
sample name
E-MTAB-13557:Control3
scientific_name
Oryza sativa Japonica Group