RNA-seq of WT, 60 min after release from aF replicate 2
Saccharomyces cerevisiae
Yeast cells were grown at 25C to an OD600 of ~ 0.7 and arrested in G1 phase with -factor at a final concentration of 50 ng/mL for 2 h. At the end of -factor arrest, a sample was collected (af arrested), and then the cells were washed twice in sterile water and released into fresh media. Samples were collected at 40 and 60 minutes after af release.
strain
WT
host
Not applicable
collection_date
2021-10-11
geo_loc_name
USA
sample_type
Cell culture
genotype
MATa, leu2-3,112, BAR1::TRP, can1-100, URA3::BrdU-Inc, ade2-1, his3-11,15
mating_type
a
temp
25
replicate
2
assay_type
RNA-seq
time_point
60
BioSampleModel
Microbe, viral or environmental