metagenome
Natural grassland, Bulk Soil, Replicate VI
collection_date
2018
depth
0-15 cm
elev
57 m
env_broad_scale
Mineral
env_local_scale
Soil
env_medium
Bulk Soil
geo_loc_name
Uruguay
lat_lon
33.15544 S 54.29281 W
host_common_name
NA
host_latin_name
NA
animal_gut
NA
sequencing_platform
Illumina MiSeq
dna_extraction_method
FastDNA SPIN kit for soil
dna_extraction_amount
0.5g
agrochem_addition
faltante
al_sat
No colectado
al_sat_meth
NA
altitude
NA
annual_season_precpt
Annual mean: 1379 ± 58 mm (no seasonality within the year)
annual_season_temp
Annual Mean maximum: 23.0 ± 0.1°C, annual mean minimum: 11.3 ± 0.6 °C (January (warmest): 23.0 ± 0.5°C/ July (coldest): 10.9 ± 0.9 °C)
crop_rotation
NA
cur_land_use
NA
cur_vegetation
No colectado
cur_vegetation_meth
NA
drainage_class
poorly
extreme_event
No colectado
extreme_salinity
No colectado
fao_class
Typic Argiudolls
fire
No colectado
flooding
No colectado
heavy_metals
No colectado
heavy_metals_meth
No colectado
horizon
A horizon
horizon_meth
faltante
isolation_source
NA
link_class_info
NA
link_climate_info
http://www.inia.uy/gras/Clima/Banco-datos-agroclimatico
local_class
NA
local_class_meth
NA
microbial_biomass
No colectado
microbial_biomass_meth
NA
misc_param
NA
omics_observ_id
NA
ph
Faltante
ph_meth
Beretta et al. (2014)
pool_dna_extracts
No
previous_land_use
Natural grasslands (before 1995)
previous_land_use_meth
Faltante
salinity_meth
NA
samp_collect_device
soil core auger (2 cm diameter)
samp_mat_process
15–20 soil core samples per replicate were randomly collected between plants with a soil core auger (2 cm diameter) at 0–15 cm depth, mixed and homogenized by sieving with 5 mm mesh size (roots and stones were removed) immediately after sampling. For microbial analysis, samples were stored at −20◦C until total community DNA (TC-DNA) extraction.
samp_size
No colectado
sieving
Sieving with 5 mm mesh size
size_frac
NA
slope_aspect
NA
slope_gradient
NA
soil_type
NA
soil_type_meth
NA
source_material_id
NA
store_cond
stored at -20°C, extraction 5 moths later
texture
39% sand, 39% silt, 22% clay
texture_meth
Faltante
tot_n_meth
combustion at 900ºC and subsequent detection of N2 by thermal conductivity
tot_nitro
No colectado
tot_org_c_meth
Wright & Bailey (2001)
tot_org_carb
No colectado
water_content_soil
No colectado
water_content_soil_meth
NA
Replicate
biological replicate 6
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.soil