RNA-seq of cac1, 150 min after release from initial aF, replicate 1
Saccharomyces cerevisiae
Yeast cells were grown at 25C to an OD600 of ~ 0.7 and arrested in G1 phase with -factor at a final concentration of 50 ng/mL for 2 h. At the end of -factor arrest, a sample was collected (af arrested), and then the cells were washed twice in sterile water and released into fresh media. Samples were collected at 10-minute intervals for 60 minutes, after which -factor was added back to the media at the same concentration. A final sample was collected at 150 minutes after the initial release from -factor.
strain
cac1
host
Not applicable
collection_date
2023-01-31
geo_loc_name
USA
sample_type
Cell culture
genotype
MATa, leu2-3,112, BAR1::TRP, can1-100, URA3::BrdU-Inc, ade2-1, his3-11,15, rlf2::HIS
mating_type
a
temp
25
replicate
1
assay_type
RNA-seq
time_point
150
BioSampleModel
Microbe, viral or environmental