F_Nz_b_22
anaerobic digester metagenome
metagenome from batch anaerobic bioreactors
ENA first public
2024-04-28
External Id
SAMEA14271465
INSDC center name
INRAE
INSDC last update
2022-04-28T10:51:42Z
INSDC status
public
Submitter Id
ena-SAMPLE-TAB-28-04-2022-10:51:40:806-12771
chemical administration
40.8 mL NH3, ammonia CHEBI:16135, 10.5 g zeolite
collection_date
2020-09-22
environment (biome)
Batch anaerobic digester
environment (feature)
anaerobic mesophilic biodigester
environment (material)
anaerobic digester sludge ENVO:00004003
experimental factor
ammonia sources
geographic location (country and/or sea)
France
geographic location (latitude)
48.7496667
geographic location (longitude)
2.3178055
investigation type
bacteria_archaea
nucleic acid amplification
Kapa HiFi DNA polymerase (10 cycles)?
nucleic acid extraction
DNeasy PowerSoil DNA Elution Kit
pcr primers
553F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT
perturbation
10.5 g zeolite
project name
STABILICS
sample name
ena-SAMPLE-TAB-28-04-2022-10:51:40:806-12771
sample storage temperature
-80
sample volume or weight for DNA extraction
2
scientific_name
anaerobic digester metagenome
sequencing method
Ion Torrent PGM
target gene
16S
target subfragment
V4-V5
temperature
35