SN-06-FO-SU3-LF
uncultured eukaryote
SN-06-FO-SU3 filtered
ENA-FIRST-PUBLIC
2022-12-14
ENA-LAST-UPDATE
2022-12-14
External Id
SAMEA112169067
INSDC center alias
University of Neuchatel
INSDC center name
university of neuchatel
INSDC first public
2022-12-14T12:35:53Z
INSDC last update
2022-12-14T12:35:53Z
INSDC status
public
Submitter Id
BG243-LF
altitude
1513
broad-scale environmental context
temperate mixed forest biome [ENVO:01000212]
collection date
2019-07-17
composite design/sieving (if any)
pool of ten subsample of 100 g and sieved at 2 mm mesh size
depth
0-0.1
elevation
1513
environmental medium
soil [ENVO:00001998]|plant litter [ENVO:01000628]
geographic location (country and/or sea)
Spain
geographic location (latitude)
36.95372
geographic location (longitude)
-3.35196
geographic location (region and locality)
Capileira, Andalucia
local environmental context
soil surface layer [ENVO:04000010]|litter layer [ENVO:01000338]
negative control type
ddH20
pH
6.9
pH method
five grams of dry soil mixed in 12.5 ml of distilled water
pcr conditions
initial denaturation=95degC_5min; 35 cycles:(denaturation=94degC_30sec; annealing=47degC_45sec; elongation=72degC_1min); final elongation=72degC_10min
pcr primers
TAReuk454FWD1:CCAGCASCYGCGGTAATTCC; TAReukREV3:ACTTTCGTTCTTGATYRA
project name
Soil protist filtration sedimentation
sample material processing
soil sieving; resuspension in dH20; filtration at 150 µm mesh size; sedimentation for 30min
sample name
BG243-LF
sample weight for DNA extraction
0.3
sequencing method
MiSeq
slope aspect
23
slope gradient
23
storage conditions (fresh/frozen/other)
in LifeGuard® (Qiagen) at -20degC
target gene
18S
target subfragment
V4
total organic C method
Thermo ScientificTM FLASH 2000 CHNS elemental analyzer
total organic carbon
297.944735058626
water content
0.059292730844792
water content method
heating at 105degC for 24 hours