Human_colon_16S8117830
Homo sapiens
Protocols: For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. cDNA library preparation was were carried out according to the manufacturer's protocol.
ENA first public
2022-04-01
ENA last update
2022-04-01
External Id
SAMEA13608106
INSDC center alias
Wellcome Sanger Institute
INSDC center name
Wellcome Sanger Institute
INSDC first public
2022-04-01T00:17:49Z
INSDC last update
2022-04-01T00:17:49Z
INSDC status
public
Submitter Id
E-MTAB-9543_2:Human_colon_16S8117830
age
70 to 75
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
normal
genotype
wild type genotype
immunophenotype
Total
individual
386C
organism part
transverse colon
sample name
E-MTAB-9543_2:Human_colon_16S8117830
sex
female