CONV-RT_10Gy_Hypoxia_0.5%O2_Rep2
Homo sapiens
source_name
HEK293T
cell line
HEK293T
genotype
WT
nuclease delivery
saCas9:RAG1.1
transfection protocol
PEI; 48 hour culture post transfection
dose
10Gy
radiation facility
LINAC; Stanford
treatment protocol
Trypsinize cells 24 hour post transfection at 0.5% O2, radiation exposure, replate cells for another 24 hours and then collect gDNA
target locus
RAG1 (saCas9:RAG1.1 gRNA)
illumina sequencing
Illumina NovaSeq 6000 System; 150PE
figure displaying results for this library
Fig 4; Sup. Fig. 3-5
number of raw reads used for normalization
904,846
pipeline used for the analysis
JoinT-seq