undifferentiated human iPSCs, biol rep 2
Homo sapiens
source_name
IMR904
protocol
Cells were routinely maintained and propagated in Pluripotency Growth Master 1 (PGM1, Cellapy, Cat# CA1007500) medium on matrigel-coated plates. The matrigel working solution was prepared in DMEM/F12 (Gibco, Cat# C11330500) at 1 mg/12 ml, and the coating was conducted at 37 ℃ for at least 1 hour. Cell splitting was carried out at 1:5 with 0.5 mM EDTA (ThermoFisher, Cat# AM9260G) in PBS when the confluency reached 80%.
cell line
IMR904
cell type
induced pluripotent stem cells
genotype
WT
treatment
culture with Pluripotency Growth Master 1