ERR7738195_bin.72
uncultured Blautia sp.
This sample represents an uncultured Blautia sp. MAG assembled and binned from ERR7738195.
16S rRNAs recovered
0
16S recovered
FALSE
16S recovery software
barrnap;0.9;--kingdom bac
23S rRNAs recovered
0
5S rRNAs recovered
0
CheckM strain heterogeneity
0
ENA first public
2022-04-01
ENA last update
2022-04-01
External Id
SAMEA13523646
GTDB r202 classification
d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Blautia_A;s__Blautia_A sp000436615
INSDC center alias
Stanford University School of Medicine
INSDC center name
stanford university school of medicine
INSDC first public
2022-04-01T08:27:28Z
INSDC last update
2022-04-01T08:27:28Z
INSDC status
public
MAG coverage depth
20.11
MAG coverage software
Reads (ERR7738195) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4566895) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.
Submitter Id
REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_C-REDO_E_9_1560.72
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics.
assembly software
metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz
binning parameters
Reads from ERR7738195 were assembled into contigs (ERZ4566895). The following read sets from this study were the most similar to ERR7738195 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738195,ERR7745411,ERR7745433,ERR7746692,ERR7738556,ERR7738574,ERR7738605,ERR7738239,ERR7738200,ERR7738264. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4566895. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.
binning software
MetaBAT2 v2.15; MAGpurify v2.1.0
collection date
2014-01-28
completeness approach
CheckM marker lineage: o__Clostridiales (UID1226)
completeness score
96.53
completeness software
CheckM;1.1.2;lineage_wf
contamination score
0
contamination screening input
reads
contamination screening parameters
All reads in ERR7738195 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).
environment (biome)
terrestrial biome
environment (feature)
human-associated habitat
environment (material)
feces
geographic location (country and/or sea)
Tanzania
geographic location (elevation)
1287
geographic location (latitude)
-3.55
geographic location (longitude)
-34.58
inter-study species representative
GUT_GENOME140320
intra-study species representative
ERR7738195_bin.72
investigation type
metagenome-assembled genome
isolation source
human feces
metagenomic source
human gut metagenome
nucleic acid extraction
https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103
number of standard tRNAs extracted
44
project name
Metagenome sequencing of Hadza gut microbiome
reassembly post binning
No
reference for biomaterial
https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103
sample collection device or method
https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103
sample derived from
ERS2585814
sample name
REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_C-REDO_E_9_1560.72
sequencing method
Illumina NovaSeq 6000
tRNA extraction software
tRNAscan-SE;2.0.9;-B
taxonomic classification
GTDB-TK v1.7.0, r202 database, default parameters
taxonomic identity marker
multi-marker approach
unique standard tRNAs extracted
18