NIH_96h_200uM_rep1
Homo sapiens
TK6 cells were exposed to N-ethyl-N-nitrosourea for 24 hours followed by subculturing and recovery in fresh media for another 24 hours. At the 48-hour and 72-hour marks, cells were subcultured again followed by an additional recovery period of 24 hours. Finally, at the 96-hour mark, cells were collected and DNA was extracted for Duplex Sequencing library preparation using the Human Mutagenesis Panel (Human-50 v1.0) (TwinStrand Biosciences).
isolate
N/A
age
N/A
biomaterial_provider
N/A
sex
not applicable
tissue
N/A
cell_line
TK6
cell_type
lymphoblastoid
culture_collection
ATCC:CRL-8015
sample_type
cell culture
treatment
ENU_96h_200uM_rep1
BioSampleModel
Human