Control_NoRT_Normoxia_21%O2_Rep1 (PGB175_RLF_N008)
Homo sapiens
source_name
HEK293T
cell line
HEK293T
genotype
WT
nuclease delivery
saCas9:RAG1.1
transfection protocol
PEI; 48 hour culture post transfection
dose
0Gy
radiation facility
None
treatment protocol
Trypsinize cells 24 hour post transfection, NO radiation exposure, replate cells for another 24 hours and then collect gDNA
target locus
RAG1 (saCas9:RAG1.1 gRNA)
illumina sequencing
Illumina NovaSeq 6000 System; 150PE
figure displaying results for this library
Fig 4; Sup. Fig. 3-5
number of raw reads used for normalization
904,846
pipeline used for the analysis
JoinT-seq