424s
Mus musculus
Protocols: Mice were sacrificed when ill, i.e. displaying signs of hematopoietic malignancies including weight loss, lethargic behaviour, anaemia and dyspnea. Malignancies were dissected out and homogenised using a 100 um tissue strainer. Homogenised cell suspensions were centrifuged and taken up in 10% DMSO and 10% FBS in RPMI and stored at -80 degrees Celsius until RNA isolation. RNA was isolated using the RNeasy mini kit (Qiagen) and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). Typically 300 ng of total RNA was used as input for enrichment by NEXTflex Poly(A) Beads (Bioo Scientific) followed by library preparation using NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).
ENA-FIRST-PUBLIC
2023-11-30T00:23:40Z
ENA-LAST-UPDATE
2023-11-30T00:23:40Z
External Id
SAMEA12009098
INSDC center name
European Research Institute for the Biology of Ageing University Medical Center Groningen
INSDC first public
2023-11-30T00:23:40Z
INSDC last update
2023-11-30T00:23:40Z
INSDC status
public
Submitter Id
E-MTAB-11247:424s
age
9.1
broker name
ArrayExpress
cell_type
tumor single-cell suspension
common name
house mouse
developmental stage
adult
genotype
Mad2 PiggyBac Mx1-Cre
individual
424
isolate
not applicable
organism part
spleen
sample name
E-MTAB-11247:424s
scientific_name
Mus musculus
sex
female
strain
mixed C57BL/6 genetic background