metagenome
isolation_source
Enrichment culture
collection_date
2020-06-17
geo_loc_name
Russia: Lake Baikal, Nothern basin
lat_lon
53.8846 N 108.6152 E
samp_collect_device
bucket grab
samp_mat_process
Sediment samples were taken aseptically from central part of the bucket grab with sterile 60-ml syringes. To obtain enrichment cultures sample sediments from Fe-Mn layers were put in anaerobic laboratory bottle (1 L) with sterile Baikal water. Remaining oxygen was replaced by flushing N2 in the bottle. The samples were stored at a temperature of 7 C in refrigerator. After 8 month of cultivation, DNA was extracted by enzymatic lysis, followed by phenol-chloroform extraction.
BioSampleModel
Metagenome or environmental