Corynebacterium glutamicum
Protocols: The cells were harvested in the exponential growth phase and frozen in liquid nitrogen. Total RNA was isolated from 3 biological replicates of C. glutamicum cells by a Quick-RNA Miniprep Plus kit (Zymo Research). The samples were treated with DNase (Roche Diagnostics) and RNA was purified with an RNA Clean&Concentrator-5 kit (Zymo Research). Ribosomal rRNA was removed with a Ribo-Zero rRNA Removal Kit for bacteria (Illumina). The purity of RNA was then tested with an Agilent RNA Pico 6000 kit and an Agilent 2100 Bioanalyzer (Agilent Technologies). TruSeq Stranded mRNA Sample Preparation guide (Illumina) was then used to construct the cDNA library.
ENA-FIRST-PUBLIC
2023-01-20
ENA-LAST-UPDATE
2023-01-20
External Id
SAMEA112302866
INSDC center alias
Omics Core Facility NGS Unit Medical School OWL & CeBiTec Bielefeld University
INSDC center name
Omics Core Facility NGS Unit Medical School OWL & CeBiTec Bielefeld University
INSDC first public
2023-01-20T00:18:10Z
INSDC last update
2023-01-20T00:18:10Z
INSDC status
public
Submitter Id
E-MTAB-12457:Wt_I
broker name
ArrayExpress
genotype
wild type genotype
growth condition
exponential phase
replicate
1
sample name
E-MTAB-12457:Wt_I