Homo sapiens
Protocols: Blood was collected in Vacutainer CPT tubes (BD), followed by centrifugation at 1800 x g for 20min. PBMCs were washed with PBS three times and stored at -135 oC. Antigen-specific B cells were purified by staining with fluorescent-labeled DNA-barcoded antigen and antibodies using FACS. Single-cell suspensions of sorted B cells were loaded onto the Chromium Controller microfluidics device (10x Genomics) and processed using the B cell Single Cell V(D)J solution according to the manufacturer's protocol. For amplify and purify the antigen barcode libraries, 10x Genomics single-cell VDJ human B cell assay and target enrichment protocol were completed.
ENA first public
2024-05-18
External Id
SAMEA14515674
INSDC center name
KOTAI Biotechnologies, Inc.
INSDC last update
2022-06-06T09:54:49Z
INSDC status
public
Submitter Id
E-MTAB-11775:1788_covid_libraseq_201110_sort_1
age
37, 39, 49, 50, 53
broker name
ArrayExpress
cell_type
peripheral blood mesothelial cell
common name
human
disease
COVID-19
disease staging
convalescence
individual
pool6_male x 4 female x 1
isolate
not applicable
organism part
blood
sample name
E-MTAB-11775:1788_covid_libraseq_201110_sort_1
scientific_name
Homo sapiens
sex
mixed, male, and female