Arabidopsis thaliana
Protocols: Plants of the meristem-specific INTACT reporter line were cultivated in growth chambers, in short-day (SD) conditions (8 h light/16 h dark) for 21 days and then shifted to long-day (LD) conditions (16 h light/8 h dark) to induce flowering. The growth-chamber condition was at 23°C with 65% relative humidity, and with the light at a fluence rate of 125 to 175 μmol m−2 s−1. Total nuclear RNA was extracted from approximately 10,000 nuclei of each sample using the RNeasy Micro Kit (Qiagen). Genomic DNA was removed by DNase I treatment (Thermo Scientific). Double-strand cDNA was synthesized and linearly amplified (Clontech SMARTer Ultra Low Input RNA for Illumina Sequencing-HV kit). Low Input Library Prep Kit (Clontech) was used for preparation of sequencing libraries
ENA-FIRST-PUBLIC
2023-01-03
ENA-LAST-UPDATE
2023-01-03
External Id
SAMEA3952374
INSDC center alias
Institute of Plant Genetics PAS
INSDC center name
Institute of Plant Genetics PAS
INSDC first public
2023-01-03T00:28:42Z
INSDC last update
2023-01-03T00:28:42Z
INSDC status
public
Submitter Id
E-MTAB-4693:Sample 5
broker name
ArrayExpress
common name
thale cress
growth condition
long day
organism part
apical shoot meristem
replicate
2
sample name
E-MTAB-4693:Sample 5
sampling time point
1
scientific_name
Arabidopsis thaliana