Homo sapiens
Protocols: Human Tregs and CD4+ cells were isolated using were isolated with EasySep™ Human CD4+CD127lowCD25+ regulatory T-cell kits (Stemcell Technologies) Primary Tregs were cultured in cRPMI with 10ng/mL mIL-2 (mouse) or 5ng/mL hIL-2 (human) Primary Tregs were cultured in cRPMI with 10ng/mL mIL-2 (mouse) or 5ng/mL hIL-2 (human), in the additional presence of 5uM ASOs as indicated. Human Tregs were additionally activated with Dynabeads Human T-Activator CD3/CD28 beads (Thermofisher) for the final two days of culture.Tregs were isolated from human PBMCs from 5 different donors, subjected to one of 4 different treatments: (1) Untreated, (2) Control ASO 792169, (3) FOXP3 ASO 910959, (4) FOXP3 ASO 910956 under either stimulation with aCD3/aCD28 coated beads or left unstimulated. Qiagen RNeasy micro kit to purify the RNA SMARTer Library prep kit with cDNA amplificationSingle endIllumina sequencing25 million reads and unstranded.
ENA first public
2022-04-02
ENA last update
2022-04-02
External Id
SAMEA13657607
INSDC center alias
Early Oncology R&D, Astrazeneca
INSDC center name
Early Oncology R&D, Astrazeneca
INSDC first public
2022-04-02T00:18:38Z
INSDC last update
2022-04-02T00:18:38Z
INSDC status
public
Submitter Id
E-MTAB-11589:28_Donor4_D_S390
broker name
ArrayExpress
cell type
regulatory T cell
common name
human
developmental stage
adult
disease
not available
individual
Donor4
organism part
blood
sample name
E-MTAB-11589:28_Donor4_D_S390
sex
not available