Mus musculus
Protocols: HUVEC-RFP (HR) cells were plated overnight in EGM-2 medium in T75 flask along with Gl261-GFP (GG) at a seeding density ranging from 100,000 to 500,000 cells such that they reach a ratio of 3:1 or 1:3 (HR: GG) post 72 hours. The cells were trypsinized and suspended in fresh EGM-2 medium. A small fraction of it was subjected to FACS analysis to establish the exact ratio between HR and GG. For appropriate control (Sep), HR and GG were grown separately in EGM-2 medium and were mixed in the identical ratio as that of co-cultured cells. The samples were quickly lysed and RNA was extracted for transcriptomics. RNA from the cells was extracted using Trizol reagent (Sigma). The cDNA synthesis was performed using RNeasy mini kit (Qiagen). ). Libraries were prepared and sequenced using Illumina directional RNA sequencing protocol (Hi-Seq).
ENA first public
2022-04-01
ENA last update
2022-04-01
External Id
SAMEA7368291
INSDC center alias
HEBREW UNIVERSITY OF JERUSALEM
INSDC center name
HEBREW UNIVERSITY OF JERUSALEM
INSDC first public
2022-04-01T00:14:58Z
INSDC last update
2022-04-01T00:14:58Z
INSDC status
public
Submitter Id
E-MTAB-9610:Sample 3
broker name
ArrayExpress
cell line
GL261-GFP
common name
house mouse
developmental stage
adult
disease
glioblastoma multiforme
genotype
wild type genotype
growth condition
GL261 and HUVEC-RFP 3:1 ratio coculture
organism part
brain
sample name
E-MTAB-9610:Sample 3
strain
C57BL/6