Manis pentadactyla
The Chinese pangolin (Manis pentadactyla) is native to the northern Indian subcontinent, northern parts of Southeast Asia and southern China. It is listed as critically endangered on the IUCN Red List since 2014, as the wild population is estimated to have declined by more than 80% in three pangolin generations, equal to 21 years. It is threatened by poaching for the illegal wildlife trade (from Wikipedia entry). This sample is from a cultured fibroblast cell line generated by William J Murphy in 2007, expanded for this project in 2020, and originally collected from the skin a male at Taipei Zoo, in Taipei, China, and then used to generate a high-quality reference for a COVID-19 project and the Vertebrate Genomes Project (VGP). Karyotyping, by Terje Raudsepp at Texas A&M University Molecular Cytogenetics core, revealed little to no polyploidy in the cultured cells. Sequencing and genome assembly were conducted at the Vertebrate Genomes Lab (VGL) at the Rockefeller University, led by Olivier Fedrigo and Erich D. Jarvis, with support by Jarvis from a COVID-19 research project. The genome is freely available to use upon public release in NCBI.
isolate
mManPen7
dev_stage
adult
sex
male
tissue
cultured skin fibroblast
biomaterial_provider
William J. Murphy, Texas A&M University
cell_line
skin cell line fibroblast
collected_by
Taipei Zoo (original animal)
collection_date
2007-01-09
geo_loc_name
Taiwan: Taipei Zoo, China
lat_lon
24.9986 N 121.5810 E
store_cond
-80oC
treatment
frozen
Cells passages used for mManPen7 assembly
passage 4 (Pacbio sequencing), passage 8 (Hi-C); 2x10^6 cells
Culture collection date
July 3, 2020
BioSampleModel
Model organism or animal