Homo sapiens
Protocols: Human Tregs and CD4+ cells were isolated using were isolated with EasySep™ Human CD4+CD127lowCD25+ regulatory T-cell kits (Stemcell Technologies) Primary Tregs were cultured in cRPMI with 10ng/mL mIL-2 (mouse) or 5ng/mL hIL-2 (human) Primary Tregs were cultured in cRPMI with 10ng/mL mIL-2 (mouse) or 5ng/mL hIL-2 (human), in the additional presence of 5uM ASOs as indicated. Human Tregs were additionally activated with Dynabeads Human T-Activator CD3/CD28 beads (Thermofisher) for the final two days of culture.Tregs were isolated from human PBMCs from 5 different donors, subjected to one of 4 different treatments: (1) Untreated, (2) Control ASO 792169, (3) FOXP3 ASO 910959, (4) FOXP3 ASO 910956 under either stimulation with aCD3/aCD28 coated beads or left unstimulated. Qiagen RNeasy micro kit to purify the RNA SMARTer Library prep kit with cDNA amplificationSingle endIllumina sequencing25 million reads and unstranded.
ENA first public
2022-04-02
ENA last update
2022-04-02
External Id
SAMEA13657615
INSDC center alias
Early Oncology R&D, Astrazeneca
INSDC center name
Early Oncology R&D, Astrazeneca
INSDC first public
2022-04-02T00:18:38Z
INSDC last update
2022-04-02T00:18:38Z
INSDC status
public
Submitter Id
E-MTAB-11589:35_Donor5_C_S397
broker name
ArrayExpress
cell type
regulatory T cell
common name
human
developmental stage
adult
disease
not available
individual
Donor5
organism part
blood
sample name
E-MTAB-11589:35_Donor5_C_S397
sex
not available