Homo sapiens
Protocols: TECs and NECs were isolated from 12 human CRC patients according to Naschberger et al, JoVE 2018. PBMCs were obtained from whole blood from the same patients and corresponding tumor DNA was extracted from microdissected tumor cells from FFPE-tissue blocks. Total DNA was extracted from TECs or NECs using the QIAamp DNA mini kit (Qiagen). DNA quantity was determined using a Nanodrop 2000c. Extraction of genomic DNA from PBMCs derived from fresh blood was performed by magnetic bead based DNA extraction with the Chemagen chemagic 360 instrument (Perkin-Elmer, Waltham, MA, USA). Tumor DNA was isolated from FFPE tissue after microdissection as previously described (Stöhr et al, Pathobiology 2019). Whole Exome Enrichment and Library Prep was performed with SureSelect version 6 (Agilent).
ENA first public
2022-04-01
ENA last update
2022-04-01
External Id
SAMEA9009627
INSDC center alias
Division of Molecular and Experimental Surgery, University Medical Center Erlangen
INSDC center name
Division of Molecular and Experimental Surgery, University Medical Center Erlangen
INSDC first public
2022-04-01T00:15:31Z
INSDC last update
2022-04-01T00:15:31Z
INSDC status
public
Submitter Id
E-MTAB-10521:Sample 9
broker name
ArrayExpress
cell type
endothelial cell
common name
human
disease
colorectal carcinoma
individual
TRC55
organism part
colon
sample name
E-MTAB-10521:Sample 9
sampling site
neoplasm