Corynebacterium glutamicum
Protocols: The cells were harvested in the exponential growth phase and frozen in liquid nitrogen. Total RNA was isolated from 3 biological replicates of C. glutamicum cells by a Quick-RNA Miniprep Plus kit (Zymo Research). The samples were treated with DNase (Roche Diagnostics) and RNA was purified with an RNA Clean&Concentrator-5 kit (Zymo Research). cDNA library enriched for primary 5′-transcripts according to Peifer-Sancar et al. (2013): I) Depletion of stable RNAs using the Ribo-Zero rRNA removal kit II) RNA fragmentation by metal hydrolysis III) Digestion of non native 5'-Mono/Di-phosphate ends by Terminator 5′-phosphate-dependent exonuclease IV)Conversion of 5′-triphosphates of primary transcript ends to monophosphates to enable adapter ligation V)Full RNA adapter ligation VI)cDNA synthesis by the usage of a loop adapter which hybridizes via a 3′-NNNNNNN-tail to the 3′-end of the RNA fragment VI)Amplification and Indexing by Adapter-specific primer
ENA-FIRST-PUBLIC
2023-01-20
ENA-LAST-UPDATE
2023-01-20
External Id
SAMEA112302885
INSDC center alias
Omics Core Facility NGS Unit Medical School OWL & CeBiTec Bielefeld University
INSDC center name
Omics Core Facility NGS Unit Medical School OWL & CeBiTec Bielefeld University
INSDC first public
2023-01-20T00:18:10Z
INSDC last update
2023-01-20T00:18:10Z
INSDC status
public
Submitter Id
E-MTAB-12458:Deletion mutant cseE
broker name
ArrayExpress
growth condition
exponential phase
sample name
E-MTAB-12458:Deletion mutant cseE
strain
deletion mutant cseE