Mus musculus
Protocols: Microglia were immunomagnetically isolated according to the manufacturer's instructions (Miltenyi Biotec, Germany). Briefly, mouse brain cortices were dissected and minced on ice. The minced tissues were enzymatically dissociated using Adult Brain Dissociation Kit (Miltenyi Biotec, Germany) and further mechanically dissociated by the gentleMACS Octo Dissociator (Miltenyi Biotec, Germany). Tissue debris and red blood cells were subsequently removed with multiple rounds of incubation and centrifugation as instructed in the manufacturer's guidelines. The prepared single-cell suspensions were immediately incubated with anti-CD11b (Microglia) MicroBeads (Miltenyi Biotec, Germany) and separated using MACS LS columns (Miltenyi Biotec, Germany) in a magnetic field of a QuadroMACS Separator (Miltenyi Biotec, Germany). Cells in the CD11b+ fraction were collected and used for the subsequent experiments. Total RNA was isolated using RNeasy Plus Micro Kit and automatically processed on a QIAcube Connect system (QIAGEN, Germany). The library preparation and subsequent sequencing were conducted with TruSeq Targeted RNA Custom Panel Kit, TruSeq Targeted RNA Index Kit A.
ENA-FIRST-PUBLIC
2022-04-01T00:15:27Z
ENA-LAST-UPDATE
2022-04-01T00:15:27Z
External Id
SAMEA8693417
INSDC center name
Ludwig-Maximilian University Munich Center for Neuropathology
INSDC first public
2022-04-01T00:15:27Z
INSDC last update
2022-04-01T00:15:27Z
INSDC status
public
Submitter Id
E-MTAB-10422:195
age
4
broker name
ArrayExpress
cell type
microglial cell
common name
house mouse
developmental stage
adult
genotype
wild type genotype
immunophenotype
CD11b positive
organism part
brain
sample name
E-MTAB-10422:195
scientific_name
Mus musculus
sex
female
strain
BL6