Homo sapiens
Protocols: HL-60 cells were treated or not with 2µM Ara-C, 1µM DNR or 1µM DNR + 20µM VAS2870 for 2hours, CHIP was performed using SUMO-2/3 goat polyclonal antibody Library preparation was performed using the Truseq ChIP Sample Preparation kit from Illumina (ref. IP-202-1012) according to the manufacturer's instructions. For each sample, 10ng of DNA was repaired, resulting in blunt end fragments and adenylated on its 3' ends. Illumina's adapters were ligated on adenylated DNA. A 18-cycles PCR was performed on the libraries using Illumina's PCR primers. The libraries were size selected at 300pb (+/-50pb) on a 2% agarose gel. Quality control of the libraries was performed on a High sensitivity DNA chip on a BioAnalyzer (Agilent) and libraries were quantified using the KAPA Library quantification kit (Clinisciences, ref. KK4824).
ENA-FIRST-PUBLIC
2023-01-03
ENA-LAST-UPDATE
2023-01-03
External Id
SAMEA4350592
INSDC center alias
Institut de Genetique Moleculaire de Montpellier CNRS - Universite de Montpellier
INSDC center name
Institut de Genetique Moleculaire de Montpellier CNRS - Universite de Montpellier
INSDC first public
2023-01-03T00:28:43Z
INSDC last update
2023-01-03T00:28:43Z
INSDC status
public
Submitter Id
E-MTAB-4947:Sample 1
broker name
ArrayExpress
cell_line
HL-60
common name
human
sample name
E-MTAB-4947:Sample 1
scientific_name
Homo sapiens