Arabidopsis thaliana
Protocols: All Arabidopsis thaliana plants used in this study were of the ecotype Col-0. Plants were grown for 1 week in a growth chamber (200 μEm-2s-1, 23°C, 16 h light, 70% relative humidity). Seeds were sterilized by 2 min incubation with 12% sodiumhypochloride (Roth, Germany) and sown on UV-sterilized hardware. Seedlings were grown in a hydroponic system, called ARADISH (Schuler et al., 2015), on stainless steel meshes with water, but without media. Seeds were sown within a one cm radius with respect to the center of the dish and the center of clinorotation and ARADISHES were placed in a phytochamber (Binder). A. thaliana seedlings, grown on ARADISH were either exposed to 5 to 600 minutes of 2D clinorotation at 60 revolutions per minute (rpm) and 21 °C in the dark on a custom-built clinostat (DLR, Cologne, Germany) or kept in darkness and used as a contol. After exposure the roots were harvested in liquid nitrogen. RNA was extracted from 100 mg root tissue per replicate using an RNeasy Plant Mini Kit (QIAGEN) according to the manufacturer's instructions. rRNA removal was performed with the Ribo-Zero Plant rRNA removal kit (Illumina) for plant seeds and roots according to the manufacturer's instructions. RNA library preparation was performed using the Illumina TruSeq® Stranded Total RNA LT kit following the manufacturer's protocol.
ENA-FIRST-PUBLIC
2023-01-03
ENA-LAST-UPDATE
2023-01-03
External Id
SAMEA4052049
INSDC center alias
Westfalische Wilhelms Universitat
INSDC center name
Westfalische Wilhelms Universitat
INSDC first public
2023-01-03T00:28:43Z
INSDC last update
2023-01-03T00:28:43Z
INSDC status
public
Submitter Id
E-MTAB-4873:mg-30-2
broker name
ArrayExpress
common name
thale cress
ecotype
Col0
organism part
root
sample name
E-MTAB-4873:mg-30-2
scientific_name
Arabidopsis thaliana
stimulus
microgravity
time
30