Mus musculus
Protocols: They are male adult ICR mice (wild type) and kept under temperature 22°C, 12 h light/dark cycle with free access to standard rodent chow and water. Sham：Muscle samples were from sham group. Sham group in which PBS was injected into the tibialis anterior muscle for 14 days；Den14d：Muscle samples were from Den group. Den group in which PBS was injected into the tibialis anterior muscle after sciatic nerve injury for 14 days；EVs14：Muscle samples were from SKP-SC-EVs group. SKP-SC-EVs group in which PBS containing SKP-SC-EVs was injected into the tibialis anterior muscle after sciatic nerve injury for 14 days. 1. sciatic nerve transection protocol - The mouse skin was sterilized and dissected in the surgical area of the left thigh, the muscle was bluntly separated to expose the sciatic nerve, and then the sciatic nerve was transected 10 mm. 2. SKP-SC-EVs treatment protocol - extracellular vesicles derived from skin-derived precursors-derived Schwann cells. mouse grow in different time mouse sample from different time Total RNA was extracted using Trizol LS. Cells were fractionated and cytoplasmic RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions mRNA-Seq libraries (from polyA+ cytoplasmic RNA) were created according to the standard Illumina protocol.
ENA-FIRST-PUBLIC
2022-12-17
ENA-LAST-UPDATE
2022-12-17
External Id
SAMEA112197318
INSDC center alias
The Lab of OE Biotech
INSDC center name
The Lab of OE Biotech
INSDC first public
2022-12-17T00:40:47Z
INSDC last update
2022-12-17T00:40:47Z
INSDC status
public
Submitter Id
E-MTAB-12425:Sham_3
age
2
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
Sham_3
isolate
not applicable
organism part
skeletal muscle tissue
sample name
E-MTAB-12425:Sham_3
sex
male
strain
BALB/c