Homo sapiens
Protocols: Total RNA was extracted from U2OS cells 72 hours after siRNA transfection and 3 hours following treatment with pladienolide B inhibitor (5 nM) (3 independent biological replicates per condition). U2OS cells were treated with siRNA-mediated depletion of MDC1 and PLRG1, or treated with the splicing inhibitor pladienolide B (5 nM). Cells were treated with control siRNA as control condition. Total RNA extraction was carried out using RNeasy Mini Kit (QIAGEN) according to manufacturer's instructions. Oxford Nanopore Technologies (ONT) kits SQK-PCS109 and SQK-PBK004 were used for preparation of the barcoded cDNA library according to the manufacturer. The initial input was 70 ng of total RNA per sample. cDNAs were amplified using 18 PCR cycles.
ENA-FIRST-PUBLIC
2023-01-20
ENA-LAST-UPDATE
2023-01-20
External Id
SAMEA112303563
INSDC center alias
Danish Cancer Society Research Center, DNA Replication and Cancer Group
INSDC center name
Danish Cancer Society Research Center, DNA Replication and Cancer Group
INSDC first public
2023-01-20T00:18:10Z
INSDC last update
2023-01-20T00:18:10Z
INSDC status
public
Submitter Id
E-MTAB-12497:BC05
age
15
broker name
ArrayExpress
cell_line
U2OS
cell_type
epithelial cell
common name
human
developmental stage
juvenile stage
disease
osteosarcoma
genotype
wild type genotype
organism part
tibia
sample name
E-MTAB-12497:BC05
sex
female