Caenorhabditis elegans
Protocols: Standard conditions were used for C. elegans propagation at 20 oC (Brenner, 1974). Animals were synchronized by hypochlorite bleaching, hatched overnight in M9 buffer at 20 °C and subsequently cultured on NGM agar plates seeded with OP50. Worms were grown from L1 until adulthood D1 at either 20 °C or 25 °C. The following genotypes were used: OW1601: dvIs62[snb-1p::hTDP-43/3'long UTR + mtl-2p::GFP]X and OW1603: dvIs15[(pPD30.38)unc-54 (vector) + (pCL26)mtl-2p::GFP]. OW1601 is CL6049 and OW1603 is CL2122 but then backcrossed an additional 6 times. CL2122 and CL6049 were gifts from Chris Link Total RNA from Day 1 adult C. elegans was isolated using TRizol (Invitrogen; #15596018) according manufacturer's protocol. The RNA concentration was assessed with a NanoDrop 2000 spectrophotometer (Thermo Scientific) and to assess the RNA quality, a 2100 BioAnalyzer (Agilent) was used to determine the RNA integrity score (RIN score) of all samples (Agilent; RNA 6000 Pico kit; #5067-15130) Next, rRNA was depleted from all samples with the NEXTflex RiboNaut rRNA depletion kit (PerkinElmer; #Nova-512961) starting with 800ng of total RNA. The cDNA library was made with the NEXTflex Rapid Directional qRNA-seq kit- Set B (PerkinElmer; #Nova-5130-04D).
ENA first public
2022-04-01
ENA last update
2022-04-01
External Id
SAMEA8521024
INSDC center alias
European Research Institute for the Biology of Ageing, University of Groningen, UMC Groningen
INSDC center name
European Research Institute for the Biology of Ageing, University of Groningen, UMC Groningen
INSDC first public
2022-04-01T00:15:23Z
INSDC last update
2022-04-01T00:15:23Z
INSDC status
public
Submitter Id
E-MTAB-10325:Control_20C_R2
age
1
broker name
ArrayExpress
developmental stage
adult
genotype
wild type genotype
organism part
whole organism
sample name
E-MTAB-10325:Control_20C_R2
strain
OW1603
temperature
20