Homo sapiens
Protocols: DNAse treated total RNA samples dissolved in nuclease free water NL20 cells were transfected with ALK-fusion constructs (pLVX-TetOne-EML4-ALK-V1, pLVX-TetOne-EML4-ALK-V3, pLVX-TetOne-KIF5B-ALK and pLVX-TetOne-TFG-ALK) or pLVX-TetOne (empty vector) respectively using X-tremeGENE™ HP DNA transfection reagents according to the manufacturer's instruction. After transfection, cells were selected with 1µg/ml puromycin (ThermoFisher) to generate stable cell lines. Promega Total RNA Isolation Kit Eukaryotic Transcriptome Library
ENA-FIRST-PUBLIC
2022-12-18
ENA-LAST-UPDATE
2022-12-18
External Id
SAMEA12303336
INSDC center alias
Sahlgrenska Academy, Gothenburg University
INSDC center name
Sahlgrenska Academy, Gothenburg University
INSDC first public
2022-12-18T00:31:06Z
INSDC last update
2022-12-18T00:31:06Z
INSDC status
public
Submitter Id
E-MTAB-11304:V1D1_1
broker name
ArrayExpress
cell_line
NL20
cell_type
bronchial epithelial cell
common name
human
disease
normal
genotype
pLVX-TetOne-EML4-ALK-V1
isolate
not applicable
organism part
lung
phenotype
wild type
sample name
E-MTAB-11304:V1D1_1
stimulus
none