Homo sapiens
isolate
Cancer cell line
age
passage 8
biomaterial_provider
Johan Staaf, Translational Cancer Research, Laboratory Medicine, Lund University, Scheelevagen 2, 22381 Lund, Sweden
collection_date
2020-09
geo_loc_name
Sweden: Lund
sex
female
tissue
Breast
cell_line
SUM185PE
disease
Breast cancer
Culture information
Cultured in Hams F-12 (GE Healthcare HyClone) supplemented with hydrocortisone (1 g/ml, Sigma-aldrich), insulin (5 g/ml, Sigma-aldrich), HEPES (10 mM, Sigma-Aldrich) and 5% FBS, but without antibiotics. Cultured under standard culturing conditions (37oC, 5% CO2). Sent for mycoplasma testing (Eurofin Genomics) and confirmed as mycoplasma free. Sent for short tandem repeat (STR) profiling (Eurofins Genomics) to confirm cell line origin.
BioSampleModel
Human