soil metagenome
The present work monitored, over a decade, the cumulative effect of repeated digestate application on soil microbial communities employing and in-situ approach, comparing the effect of the digested feedstock to the same undigested feedstock, and to others fertilization treatments. The study was carried out at the experimental field site EFELE, located in Rennes, France. The experiment was set up in 2012 and is based on a wheat-maize crop rotation. A mineral fertilizer (MIN), and three different OWP: cattle manure (CM), pig slurry (PS), and pig slurry anaerobic digestate (PS-DIG), were applied once every year since the beginning of the trial to the present. These treatments were compared to a control (0N) that had not received any organic or mineral N input. To monitor soil microbial community dynamics, from 2012 to 2022, soil samples were collected every year twelve months after the last fertilization treatment. For the molecular analyses performed in this study, were selected soil samples belonging to the beginning of the trial (2012), and wheat crop years (2013, 2015, 2017, 2019, and 2022). All the molecular analyses were conducted in 2022; we used high-throughput sequencing targeting 16S and 18S ribosomal RNA genes to describe the dynamics of microbial soil communities. Our results highlighted that over a decade of annual inputs of OWPs or mineral fertilizer, the soil chemical properties, the microbial biomass, and the composition and structure of soil microbial communities followed lasting modifications in all evaluated treatments. These changes were primarily explained by the time effect and, to a lesser extent, by the quality of the applied product. The temporal factor (year) exerted a strong influence on all measured parameters, potentially reflecting environmental, climatic, and seasonal effects that could mask the impact of digestate on soil microorganisms. Moreover, soil management practices appear to buffer the effects of different treatments, whether organic or mineral, on soil microbial quality indicators. The impact of digestate on soil microbial communities should be assessed considering associated agricultural practices.
Block
1
ENA first public
2024-01-01
External Id
SAMEA115029630
INSDC center name
INRA-CMSE
INSDC last update
2023-12-14T10:47:30Z
INSDC status
public
Submitter Id
EFELE_2017_03_PRO_109_18S
Treatment
CM
broad-scale environmental context
terrestrial environmental zone
collection date
2017
depth
0.2
elevation
34
environmental medium
agricultural soil
geographic location (country and/or sea)
France
geographic location (latitude)
48.102
geographic location (longitude)
-1.7956
local environmental context
area of cropland
multiplex identifiers
F: 5'-CACGTAGATC-3'; R: 5'-GACGAGCTAC-3'
pcr primers
F: 5'-CGATAACGAACGAGACCT-3'; R: 5'-ANCCATTCAATCGGTANT-3'
project name
PRJEB71034
sample name
EFELE_2017_03_PRO_109_18S
sample volume or weight for DNA extraction
1
scientific_name
soil metagenome
target gene
18S