Mus musculus
Protocols: 4T1 breast cancer cells were cultured on soft (1.24±1.3kPa ) and stiff (13.20±2.3 kPa) two-dimensional polyacrylamide hydrogels for 24hrs before being harvested for RNA extraction 4T1 breast cancer cells were cultured on soft (1.24±1.3kPa ) two-dimensional polyacrylamide hydrogels for 24hrs RNA extraction was performed using the Qiagen RNAeasy extraction kit as per manufacturers recommendations The quality of the RNA was confirmed using TapeStation (Agilent), prior to library preparation using the KAPA mRNA HyperPrep Kit, as per the manufacturer protocol. Quality control was performed on the mRNA library using a LabChip GX prior to RNA sequencing.
ENA first public
2024-01-01
External Id
SAMEA13204333
INSDC center name
Garvan Institute of Medical Research and The Kinghorn Cancer Center, Darlinghurst, NSW, Australia
INSDC last update
2022-03-01T17:51:02Z
INSDC status
public
Submitter Id
E-MTAB-11487:SoftHydrogel_4T1_RNAseq_rep2
broker name
ArrayExpress
cell_line
4T1
common name
house mouse
developmental stage
adult
disease
breast carcinoma
growth condition
cultured on soft (1.24±1.3kPa) two-dimensional polyacrylamide hydrogels for 24 hours
isolate
not applicable
organism part
mammary gland
sample name
E-MTAB-11487:SoftHydrogel_4T1_RNAseq_rep2
scientific_name
Mus musculus
strain
BALB/c