Homo sapiens
Protocols: Cells were treated with 2 μg/ml doxycycline and 0.06 μg/ml nocodazole, 24 and 18 h respectively, before the addition of 1 mM IAA to induce degradation of mAID-tagged proteins. After 4 h treatment with IAA, mitotic cells were manually detached by shake-off, and cells were transferred to new poly-L-lysin coated dishes. Once attached, cells were washed 3x with PBS and released from mitosis into G1 for 18 h in a fresh medium containing 2 mM thymidine.Cells were washed with PBS, detached with TrypLE and pelleted by centrifugation. Total RNA was extracted from HCT116 cells (G1, -/+ mAID-protein of interest) using Monarch Total RNA Miniprep Kit (New England Biolabs), according to manufacturer's instructions. Total RNA were sent in dry-ice package to Macrogen Europe B.V. Macrogen Europe BV constructed libraries using Illumina TruSeq stranded mRNA library preparation with Ribozero rRNA depletion.
ENA first public
2024-01-01
INSDC center name
College of Medicine, Health and Life Science, Brunel University London, Center of Genomic Engineering and Maintenance (CenGem)
INSDC status
public
Submitter Id
E-MTAB-13827:Repo-Man_CTRL
broker name
ArrayExpress
cell_line
HCT116
cell_type
colorectal cancer cell
collection date
not collected
common name
human
developmental stage
adult
disease
colorectal carcinoma
genotype
AID-degron Repo-Man
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
colon
sample name
E-MTAB-13827:Repo-Man_CTRL
scientific_name
Homo sapiens