Mus musculus
Protocols: Male and Female C57Bl/6NTac were purchased from Taconic. All mice were kept in a standard temperature- and humidity-controlled environment with a 12h:12h light-dark cycle. Mice had nesting material and ad libitum access to water and commercial diets. For the studies in Figures 1-3, mice were fed a regular mouse housing diet (Safe®150). For the high-fat diet study, we used D12492 from Research Diets, while using D12450J as a sucrose matched control. NRH was provided to mice in the drinking water, at a concentration of 0.1% of 0.4%, which approximately leads to a dose of 100 mg/kg*day or 400 mg/kg*day assuming mice drink around 3 ml per day and weight around 30 grams. Water bottles were renewed twice a week with a fresh solution. For the high-fat diet, the dose of NRH in the water bottles was adjusted weekly according to the average body weight of the cage. All animal experiments were carried out according to national Swiss and EU ethical guidelines and approved by the local animal experimentation committee under licenses VD2770.1, VD3647 and VD3841.  Animals were sacrificed after a 2 h fast, in order to stabilize systemic parameters and to allow the measurement of blood biochemistry in the fasting state. Tissues were collected upon sacrifice and flash-frozen in liquid nitrogen. Total mRNAs were extracted as described previously (Ratajczak, J. et al. Nat Commun, 2016) BRB-seq libraries were generated using a MERCURIUS BRB-seq kit (Alithea Genomics, #10813) according to the manufacturers' instructions
strain
C57Bl/6NTac
treatment
NRH400
organism
Mus musculus
organism
Mus musculus
collection date
not collected
sex
male
batch
20240222
scientific_name
Mus musculus
tissue
epididymal White Adipose Tissue
alternate name
eWAT_400-4
common name
house mouse
organism part
epididymal White Adipose Tissue
type
Demultiplexed sample
developmental stage
adult
genotype
wild type genotype
isolate
not applicable
library
20240222_BRBseq_eWAT_HFD
brbseq barcode kit
384 V5A5 Odt
diet
HFD
barcode sequence
TAACAGTCCGTTCA
barcode
L16
age
8
geographic location (country and/or sea)
not collected