Zea mays
corn was planted with four individual weeds (canola) surrounding a corn seed in the center of an 8 pot. The weeds were planted outside conetainers so that there was direct root-to-root contact between the corn and the canola. To block the above-ground weed-generated signals, an opaque plastic cone-shaped barrier was placed, point down, over the corn seedling to prevent any weed-generated light quality (increased far-red light due to reflection from the canola) signal from impacting the corn seedling. This cone was enlarged as the corn and canola grew so that any above-ground light quality signal was blocked until after the corn had reached the V8 stage of growth. The plants were grown under greenhouse conditions with diffuse light and an 18 Hr photoperiod at about 25 C for 3 months (until the plants were at the V12 stage of growth). Plants were watered daily and fertilized weekly by additional watering with 20:20:20. Plant root (including roughly equal amount by weight of support roots and horizontal roots) and leaf tips (the distal 10 cm of the top-most fully expanded leaf) were harvested separately into liquid nitrogen for future RNA extractions.
isolate
84 day
cultivar
commercial hybrid
ecotype
NA
age
3 months
dev_stage
V12
collection_date
2022-10-22
geo_loc_name
USA: Fargo, ND
tissue
mix or lateral and support roots
biomaterial_provider
David Horvath
collected_by
Laura Kelley
temp
20-25C
treatment
weedy with only above-ground barriers
BioSampleModel
Plant