Homo sapiens
Protocols: Cells were treated with 2 μg/ml doxycycline and 0.06 μg/ml nocodazole, 24 and 18 h respectively, before the addition of 1 mM IAA to induce degradation of mAID-tagged proteins. After 4 h treatment with IAA, mitotic cells were manually detached by shake-off, and cells were transferred to new poly-L-lysin coated dishes. Once attached, cells were washed 3x with PBS and released from mitosis into G1 for 18 h in a fresh medium containing 2 mM thymidine.Cells were treated 30min with DNAseI, washed with PBS, detached with TrypLE, counted and pelleted by centrifugation. Cell pellet containing 100 000 cells was sent in dry-ice package to Active Motif S.A. Cells are lysed with a nonionic detergent to yield pure nuclei.  The resulting chromatin is then fragmented and simultaneously tagmented with sequencing adapters using the Tn5 transposase to generate the ATAC-Seq library. After purification, the library can be amplified by PCR using barcoded primers.
disease
colorectal carcinoma
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
scientific_name
Homo sapiens
common name
human
organism part
colon
developmental stage
adult
genotype
AID-degron Ki-67
isolate
not applicable
cell_line
HCT116
cell_type
epithelial cell
geographic location (country and/or sea)
not collected