Mus musculus
Protocols: Mouse macrophages J774A.1 cells (ATCC, Manassas, VA, USA) were cultured in RPMI 1640 (Euroclone, Italy), supplemented with 10% (v/v) fetal bovine serum (FBS, Euroclone, South America, origin EU approved), penicillin-streptomycin solution (100 U/mL penicillin and 100 µg/mL streptomycin) (Euroclone, Italy) and grown at 37°C with 5% CO2 in a humidified incubator. Total RNA from J774.1 infected with B. abortus was isolated using Trizol following the manufacturers protocol. The concentration and quality of all isolated RNA samples were measured using the TapeStation 4200 (Agilent Technologies, USA) and Qubit 2.0 fluorometer (Thermo Fisher Scientific, USA). RNA samples that exhibited RNA integrity numbers ranging from 6.6 to 10 were selected for the library preparations. Libraries were prepared using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (Illumina, San Diego, USA) according to the manufacturers instructions, starting with 1 µg total RNA.
ENA first public
2024-09-30
INSDC center name
Department of Medicine, Surgery and Dentistry \"Scuola Medica Salernitana\", University of Salerno
INSDC status
public
Submitter Id
E-MTAB-13990:24h_2
broker name
ArrayExpress
cell_line
Mouse macrophages J774A.1 (ATCC, Manassas, VA, USA)
cell_type
macrophage
collection date
not collected
common name
house mouse
developmental stage
adult
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
ascites
sample name
E-MTAB-13990:24h_2
scientific_name
Mus musculus
sex
female
strain
BALB/cN