Sus scrofa
Protocols: Pigs either underwent cardiopulmonary resuscitation after induction of cardiac arrest (ischemia-reperfusion injury group) or no cardiac arrest (control group). The kidneys were removed and perfused with approximately 400 ml Jonosteril Infusion solution (Fresenius) and 10.000 international units Heparin-Sodium (Braun; 2147217). Samples from kidney cortex were snap frozen in liquid N2 and stored at -80 °C until further use. For each sample, 10mg frozen kidney was homogenized in 1ml TRI Reagent (Sigma-Aldrich; T9424-200ml) and 1.0 mm Zirconia/Silica Beads (BioSpec; 11079110Z) in a FastPrep FP120 (Savant) for 45s and 6.5 speed. The lysate incubated for 15 minutes and RNA was isolated with Direct-zol RNA Miniprep Kit (Zymo research; R2052) with DNase digestion according to manufacturer's protocol. Libraries were prepared using the Illumina® Stranded TruSeq® RNA  sample preparation Kit. Library preparation started with 500ng total  RNA. After poly-A selection (using poly-T oligo-attached magnetic  beads), mRNA was purified and fragmented using divalent cations under  elevated temperature. The RNA fragments underwent reverse  transcription using random primers. This was followed by second strand  cDNA synthesis with DNA Polymerase I and RNase H. After end repair and  A-tailing, indexing adapters were ligated. The products were then  purified and amplified (15 PCR cycles) to create the final cDNA  libraries. After library validation and quantification (Agilent Tape  Station), equimolar amounts of library were pooled. The pool was  quantified by using the Peqlab KAPA Library Quantification Kit and the  Applied Biosystems 7900HT Sequence Detection System.
strain
Hybride German Large White x Piétrain
individual
5
organism
Sus scrofa
organism
Sus scrofa
collection date
not collected
sex
male
scientific_name
Sus scrofa
common name
pig
organism part
kidney
developmental stage
adolescent
genotype
wild type genotype
isolate
not applicable
injury
ischemia reperfusion injury
age
3
geographic location (country and/or sea)
not collected