Homo sapiens
Protocols: Three groups of asymptomatic adults at risk of latent tuberculosis infection (household TB contacts, farm workers with occupational exposure to bovine TB and HIV-infected people attending an out-patient clinic) and living in or around Addis Ababa, Ethiopia, were invited to give a blood sample for processing. Principal exclusion criteria were age <18 years; symptoms of active TB; haemoglobin concentration <10 g/dL; and presence of any chest radiograph abnormality consistent with active TB. HIV-infected participants who did not have any contra-indication to IPT were offered a 6-month course of 300 mg isoniazid and pyridoxine 50 mg daily and invited to return to give a second blood sample for the same laboratory tests following treatment completion. 3 mL blood was collected into Tempus Blood RNA Tubes (Thermo Fisher Scientific) to stabilise total RNA for isolation and downstream gene expression analysis, and processed with Tempus Spin RNA Isolation Kits (Thermo Fisher Scientific) to obtain high-quality total RNA. TURBO DNA-free kits (Thermo Fisher Scientific) and GLOBINclear kits (Thermo Fisher Scientific) were then used to remove residual DNA and globin mRNAs, respectively. For genome-wide mRNA sequencing, complementary DNA libraries were generated using Kappa HyperPrep kits (Roche).
disease
HIVpos
individual
DWD488
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
is6110_cd34neg
0
scientific_name
Homo sapiens
common name
human
organism part
blood
is6110_cd34pos
68.952782
developmental stage
adult
isolate
not applicable
rpob_cd34neg
0
rpob_cd34pos
8.4901152
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2023-12-22