Homo sapiens
Protocols: Formalin fixed paraffin embedded (FFPE) tissue sections with minimal acute inflammation from the ileal margins were used for transcriptomic analyses. Total RNA was isolated from ileal margins with RNeasy FFPE Kit (QIAGEN) according to the manufacturer's protocol. Total RNA concentration and purity was obtained from an absorbance ratio at 260nm and 280nm. Total RNA quality was then determined by Agilent Tapestation according to manufacturer's recommendations. Library preparation was performed by the Genome Technology Access Center (GTAC) at Washington University. RNA library was prepared according to library kit manufacturer's protocol, indexed, and pooled.
sampling site
ileal resection margin
isolate
not applicable
disease
ulcerative colitis
individual
Sample 39
organism
Homo sapiens
organism
Homo sapiens
sex
scientific_name
Homo sapiens
common name
human
organism part
ileum
age
18
developmental stage
adult
ENA-LAST-UPDATE
2023-04-04