Diaphorina citri
The CLas-infected and -uninfected ACP colonies were reared in laboratory cultures maintained on a CLas host (Citrus spp.) (CLas-infected) or a CLas-immune rutaceous plant species (CLas-free). Cultures were established in 2005 from a field population collected in Polk Co., FL prior to the establishment of HLB. Cultures were reared continuously and serially transferred periodically to the same host species, Valencia sweet orange (Citrus sinensis (L.) plants, at the University of Florida Citrus Research and Education Center in Lake Alfred, FL. Five stages of nymphs (1-5 instar) and two stages of adults (teneral adult and post-teneral adult) were collected, with 150 1st-3rd instars and 100 4th-5th instars, teneral adults and post-teneral adults per sample, with 6 replicates for each, so that a comprehensive accurate representation of the transcriptome could be generated over the entire range of adult and nymph life stages.
isolate
Kuwayama
isolation_source
Polk Co., FL, USA
collection_date
2005-01
geo_loc_name
USA: Polk Co., FL
tissue
Whole body
lat_lon
28.0 N 81.9 W
BioSampleModel
Invertebrate