Homo sapiens
Protocols: Cell lysates were collected by buffer RLT from Qiagen RNEasy kit after 24 hours of serum treatment Cells were treated with 10% serum from a patient with focal segmental glomerulosclerosis when they were presenting with FSGS (AP), on vincristine treatment (OT), or in remission (R) for 24 hours.  Qiagen RNEasy kit to extract RNA from cells. Cells are lysed, scraped off vessel, then exposed to spin columns and washes to extract RNA. A gDNAse step was also included.  KAPA mRNA HyperPrep kit to prepare the H. sapiens library for sequencing
disease
focal segmental glomerulosclerosis
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
scientific_name
Homo sapiens
common name
human
organism part
cortex of kidney
developmental stage
adult
genotype
wild type genotype
isolate
not applicable
cell_line
human immortalised podocytes
cell_type
podocyte
geographic location (country and/or sea)
not collected