Homo sapiens
Protocols: Whole blood was collected from volunteers at -D3 (baseline), D0 (before vaccination), D1, D3, D7, and D10 post-vaccination and stored in Tempus blood RNA tubes (Thermofisher) at -80°C until use. Tempus blood RNA tubes were thawed at room temperature, and RNA was extracted using the Tempus spin RNA isolation kit (Thermofisher, catalog nunmber: 4380204), eluted in 90µL of RNase-free water according to the manufacturer's protocol. 1µg of total RNA was used for library preparation. poly(A) mRNA isolation was performed using Oligo(dT) beads. mRNA fragmentation was carried out using divalent cations and high temperature. Priming was performed using random primers. First strand cDNA and the second-strand cDNA were synthesized. The purified double-stranded cDNA was then treated to repair both ends and add a dA-tailing in one reaction, followed by T-A ligation to add adaptors to both ends. Size selection of adaptor-ligated DNA was then performed using DNA clean beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated.
ENA first public
2024-01-01
External Id
SAMEA114268346
INSDC center name
Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore
INSDC last update
2023-08-15T16:20:01Z
INSDC status
public
Submitter Id
E-MTAB-13265:M23_-D3
broker name
ArrayExpress
clinical history
prior YF-17D vaccination
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
individual
M23
isolate
not applicable
organism part
blood
sample name
E-MTAB-13265:M23_-D3
scientific_name
Homo sapiens