Homo sapiens
Protocols: Formalin fixed paraffin embedded (FFPE) tissue sections with minimal acute inflammation from the ileal margins were used for transcriptomic analyses. Total RNA was isolated from ileal margins with RNeasy FFPE Kit (QIAGEN) according to the manufacturer's protocol. Total RNA concentration and purity was obtained from an absorbance ratio at 260nm and 280nm. Total RNA quality was then determined by Agilent Tapestation according to manufacturer's recommendations. Library preparation was performed by the Genome Technology Access Center (GTAC) at Washington University. RNA library was prepared according to library kit manufacturer's protocol, indexed, and pooled.
ENA first public
2024-01-01
External Id
SAMEA112868529
INSDC center name
WASHINGTON UNIVERSITY
INSDC last update
2023-04-04T14:28:31Z
INSDC status
public
Submitter Id
E-MTAB-12826:Sample 19
age
55
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
ulcerative colitis
individual
Sample 19
isolate
not applicable
organism part
ileum
sample name
E-MTAB-12826:Sample 19
sampling site
ileal resection margin
scientific_name
Homo sapiens
sex
female