Homo sapiens
Protocols: Endometrial biopsies were collected with a catheter (Rampipella, RI.MOS., Mirandola, Italy) from the uterine cavity between days 21 and 23 of the menstrual cycle under sterile conditions prior to the IVF procedure.  Total RNA was isolated from endometrial tissue using the RNeasy Mini Kit reagent kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions (https://www.qiagen.com). The purity of the isolated RNA was assessed using a NanoDrop 2000c spectrophotometer (Nanodrop Technologies, Wilmington, Delaware, USA). Integrity was determined on an Agilent 2100 bioanalyzer using an RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, California, USA).  The library was prepared using the TruSeq RNA reagent kit (Illumina, San Diego, California, USA) according to the manufacturer's instructions. This protocol was used to convert the whole RNA into a fragment library for next-generation sequencing. After removal of ribosomal RNA using the Ribo-Zero rRNA approach (Illumina, San Diego, California, USA), the remaining RNA was purified, fragmented and the first strand cDNA (complementary DNA) was synthesized using reverse transcriptase and random oligonucleotide primers. This was followed by the synthesis of a second cDNA chain with deoxyuridine triphosphate nucleotides. The double-stranded DNA, which was terminated with blunt ends, was further adenylated at the 3'end in the next step. Sequences with molecular barcodes and adapters were added to the ends of the double-stranded cDNA to bind to the surface of the flow cell. Fragments with ligated adaptors at both ends were selectively enriched by 15 cycles of PCR.
ENA first public
2024-01-01
INSDC center name
Clinical institute of Genomic Medicine, University Medical Centre Ljubljana
INSDC status
public
Submitter Id
E-MTAB-14282:EN016
age
35
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
polycystic ovary syndrome
geographic location (country and/or sea)
not collected
individual
EN016
isolate
not applicable
organism part
endometrium
sample name
E-MTAB-14282:EN016
scientific_name
Homo sapiens
sex
female
treatment
liraglutide