Lactuca homblei
Plant Environment Ontology
EO_0007383
replicate
5
organism
Lactuca homblei
collection date
2021-05-04
Experimental Factor values
weeks 1-3: 0 mL of water
scientific_name
Lactuca homblei
common name
L. homblei
genotype
L. homblei
type_of_growth_facility
Conventional green house CO_715:0000190
study_contact_email
adiazb@cita-aragon.es
observation_unit_description
single_plant
protocol
Total RNA extraction from lyophilized samples coming from 12 samples (2 accessions x 2 irrigation regimes x 3 biological replicates) was performed using the NZY Total RNA Isolation kit (NZYtech Lda.-Genes and Enzymes, Lisbon, Portugal). RNA was treated with DNase using the TURBO DNA-freeTM kit (Invitrogen, Waltham, MA, USA), following the manufacturersâ€™ instructions. RNA quantity and purity were assessed with the Eukaryotic Total RNA Nanobioanalyzer Assay in a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA)
plant_anatomical_entity_name
Leaf
Experimental Factor type
Watering regime
Experimental Factor description
Treatment involving an exposure to watering frequencies.
study_contact_name
Aurora Diaz Bermudez
age
4 month
geographic location (country and/or sea)
Spain