Mus musculus
Protocols: TTF1 conditional Clone C8 was cultured for >7days with 100ng.ml-1 doxycycline (Dox100), cells were then either harvested or cultures continued for a further 3, 5 or 7 days without doxycycline (Dox0). Cultures were then either harvested or 100ng.ml-1 doxycycline readded and cultures maintained for a further 3, 5, and 7 days (Dox100-Readdition). Total RNA was extracted from these cultures and from parallel cultures of NIH3T3 using Trizol and GeneJet RNA micro kit (ThermoFisher Scientific). RNA sequencing libraries were created using the NEBNext mRNA stranded protocol (New England Biolabs), (McGill University and Genome Quebec Innovation Centre).
disease
normal
organism
Mus musculus
organism
Mus musculus
collection date
not collected
scientific_name
Mus musculus
common name
house mouse
organism part
embryonic structure
developmental stage
embryonic stage 14
genotype
wild type genotype
isolate
not applicable
growth condition
â‰¥ 7 days DMEM 10%FBS
cell_line
NIH3T3
cell_type
mouse embryonic fibroblast cell
geographic location (country and/or sea)
not collected