Mus musculus
Protocols: Organoids were collected using ice-cold Advanced DMEM/F-12 and centrifuged for 5 minutes at 190 x g at 4°C. Organoid pellets were then dispersed into single cells using TrypLE Express (Thermo Fisher Scientific) and mechanical dissociation, and filtered through a 40 µm filter. The cell suspension was centrifuged again (10 minutes at 190 x g at 4°C) and resuspended in PBS with 0.04% BSA at a concentration of 1x10^6 cells/mL. The single-cell suspensions were converted to barcoded Drop-seq libraries by using the Chromium Single Cell 3' V3.1 Library, Gel Bead & Multiplex Kit and Chip Kit (10X Genomics; Pleasanton, California, USA), aiming for an estimated 8,000 cells per library. Single-cell libraries were prepared with the Chromium Single Cell 3' V3.1 Chemistry Library Kit, Gel Bead & Multiplex Kit and Chip Kit from 10X Genomics.
strain
C57BL/6J
individual
EXT356
organism
Mus musculus
organism
Mus musculus
passage
3
collection date
not collected
sex
male
scientific_name
Mus musculus
common name
house mouse
organism part
Anterior pituitary
developmental stage
mouse postnatal
genotype
-/iDTR
isolate
not applicable
cell_type
Pituitary stem cell-derived organoids
age
2-3
geographic location (country and/or sea)
not collected