Homo sapiens
Protocols: First, frozen patient peritoneal cells were thawed and counted.  Next, Natural Killer (NK) cells were purified using the negative selection kit from Miltenyii (130-092-657), according to the manufactures protocol. In order to increase the purity, NK cells underwent a second round of purification using the positive selection kit from Miltenyii (130-050-401), according to the manufactures protocol. The purity was determined via FACS and microscopy and the NK cells were then lysed in 1 ml Trizol. RNA was isolated using Trizol/TriFast (30-2010), according to the manufacturers' protocol.  DNA was removed using the DNA-freeâ„¢ DNA Removal Kit (Ambion, AM1906), according to the manufacturers' protocol. Illumina Truseq mRNA stranded, according to manufacturers' instructions
disease
high grade ovarian serous adenocarcinoma
individual
OVCA198
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
female
scientific_name
Homo sapiens
common name
human
organism part
ascitic fluid
developmental stage
adult
isolate
not applicable
cell_type
natural killer cell
age
45 to 50
geographic location (country and/or sea)
not collected