Homo sapiens
Protocols: Polyclonal cells were generated by transducing cells with pINDUCER20-GFP-aFOS, followed by selection using G418. For RNA-seq, cells were seeded, and the following day treated with doxycycline to induce aFOS expression. 48 hours post-induction, RNA was harvested from cells. RNA was extracted using the RNeasy Plus Kit (Qiagen) RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit
phenotype
non-induced control
disease
esophageal adenocarcinoma
ENA-FIRST-PUBLIC
2023-04-07
organism
Homo sapiens
organism
Homo sapiens
ENA-LAST-UPDATE
2023-04-07
cell_line
KYAE-1
scientific_name
Homo sapiens
common name
human
organism part
esophagogastric junction
cell_type
epithelial cell
developmental stage
adult
genotype
aFOS-tet-on
ENA-FIRST-PUBLIC
2023-04-07
ENA-LAST-UPDATE
2023-04-07