Rhynchophorus phoenicis
Protocols: African Palm weevil larvae were collected from Nigeria and stored in nucleic acid preservation (NAP) buffer. Larvae were dissected and guts were separated into foregut, midgut and hindgut samples. Bacterial DNA extraction was performed from each sample in duplicates using the QIAamp DNA microbiome kit Qiagen) according to the manufacturer's protocol. DNA concentration and purity were assessed using a Nanodrop and stored at 40C until processing. The V3–V4 hypervariable region of the bacterial 16S rRNA gene was amplified from bacterial DNA. Sequencing libraries were prepared using the Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina). The libraries were purified, diluted to 6nm and pooled.
isolate
not applicable
individual
Y
organism
Rhynchophorus phoenicis
organism
Rhynchophorus phoenicis
collection date
not collected
scientific_name
Rhynchophorus phoenicis
common name
African palm weevil
organism part
midgut
developmental stage
larval stage
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2024-01-19