Mus musculus
Protocols: 4T1 breast cancer cells were cultured on soft (1.24±1.3kPa ) and stiff (13.20±2.3 kPa) two-dimensional polyacrylamide hydrogels for 24hrs before being harvested for RNA extraction 4T1 breast cancer cells were cultured on stiff (13.20±2.3kPa ) two-dimensional polyacrylamide hydrogels for 24hrs RNA extraction was performed using the Qiagen RNAeasy extraction kit as per manufacturers recommendations The quality of the RNA was confirmed using TapeStation (Agilent), prior to library preparation using the KAPA mRNA HyperPrep Kit, as per the manufacturer protocol. Quality control was performed on the mRNA library using a LabChip GX prior to RNA sequencing.
strain
BALB/c
isolate
not applicable
disease
breast carcinoma
organism
Mus musculus
organism
Mus musculus
growth condition
cultured on stiff (13.20±2.3kPa) two-dimensional polyacrylamide hydrogels for 24 hours
cell_line
4T1
scientific_name
Mus musculus
common name
house mouse
organism part
mammary gland
developmental stage
adult
ENA-LAST-UPDATE
2022-03-01