Homo sapiens
Protocols: We generated short hairpin RNA (shRNA) vectors against SLC25A13 and oligonucleotides were cloned into the pLKO-shRNA2 vector (PLKO). A nonsense scrambled shRNA sequence was used as a negative control. Lentiviral particles were produced in 293T cells. For lentiviral transduction of HUVECs, a multiplicity of infection (MOI) of 30 was used. Transductions were performed on day 0 and after 24 hours the cells were refed with fresh medium. RNA isolation experiments were performed from day 6 after transduction. Knockdown efficiency was monitored before RNA isolation.    RNA of scrambled control and SLC25A13KD ECs was extracted 6 days after seeding using the TRIzol kit (Life Technologies). Sequencing libraries were prepared with lllumina TruSeq Stranded mRNA Library prep kit according to the manufacturer's protocol. Samples were indexed to allow for multiplexing. Library quality and size range was assessed using a Bioanalyzer (Agilent Technologies) with the DNA 1000 kit (Agilent Technologies) according to the manufacturerâ€™s recommendations.
phenotype
SCR control
isolate
not applicable
organism
Homo sapiens
organism
Homo sapiens
cell_line
HUVECs
collection date
not collected
scientific_name
Homo sapiens
common name
human
cell_type
human umbilical vein endothelial cell
geographic location (country and/or sea)
not collected