Solea senegalensis
Protocols: By the end of the experiment, 3 male fish per tank (2 tanks per group) were randomly sacrificed with a lethal dose of anesthesia (1000 ppm phenoxyethanol) and testis were immediately dissected and frozen in liquid nitrogen. Testis samples were stored at -80 ºC for RNA extraction and further RNA-Seq quantification. The gonads collected were homogenized in 1 mL of TRI Reagent® (Sigma-Aldrich, Switzerland) using a tissue-lyzer Star-Beater (VWR). Total RNA was extracted following the instructions of the manufacturer and the obtained pellet was resuspended in RNase-free distilled water (Sigma-Aldrich, Switzerland). Total RNA concentrations were measured with a Nanodrop One-C spectrophotometer (Thermo Fisher Scientific, Washington, USA) and adjusted to 1 µg/µL. Total RNA quality and integrity were checked using a 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA), and only samples with an RNA integrity number (RIN) > 8 were considered for sequencing. A total of 12 libraries were prepared using a TruSeq™ RNA Library Preparation Kit v2 of Illumina (Illumina, USA).
ENA first public
2024-08-01
INSDC center name
Centre of Marine Sciences (CCMAR)
INSDC status
public
Submitter Id
E-MTAB-14218:Sample 12
broker name
ArrayExpress
collection date
not collected
common name
Senegalese sole
developmental stage
Breeder
diet
algae
geographic location (country and/or sea)
not collected
individual
12
isolate
not applicable
organism part
testis
sample name
E-MTAB-14218:Sample 12
scientific_name
Solea senegalensis
sex
male