Mus musculus
Protocols: D2A1-LifeActin-TdTomato cells (Mouse Mammary Carcinoma) were cultured at 37°/5% CO2. DMEM HG with 5% NBCS, 5% FBS, 1% NEAA-MEM, 1% Penstrep.  Cells were harvested and pelleted for RNA extraction. 37°/5% CO2. DMEM HG with 5% NBCS, 5% FBS, 1% NEAA-MEM, 1% Penstrep. siRNAs were transfected into the cells using Lipofectamine RNAiMAX (Invitrogen) following the manufacturer's instructions. Experiments were performed 72 h post-transfection. RNA extraction was performed using standar methods. RNA integrity was assessed by Bioanalyzer (total RNA Pico Kit, 2100 Instrument, Agilent Technologies, Paolo Alto, CA, USA). All samples had RNA integrity numbers above 8 and DV200>80%. Sequencing libraries were prepared using “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina“ and enriched in mRNA using “NEB Ultra II polyA m RNA magnetic isolation” kit (New England Biolabs, Ipswich, MA, USA).
ENA first public
2024-11-24
INSDC center name
Unistra
INSDC status
public
Submitter Id
E-MTAB-14571:ViscoMeta_1_916-ctrl_BM1_10_R1_M1
broker name
ArrayExpress
cell_line
D2A1-LifeActin-TdTomato
cell_type
mouse mammary carcinoma cell line
collection date
not collected
common name
house mouse
developmental stage
adult
disease
mammary carcinoma
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
mammary gland
sample name
E-MTAB-14571:ViscoMeta_1_916-ctrl_BM1_10_R1_M1
scientific_name
Mus musculus