Homo sapiens
Protocols: Jurkat and MT-2 cells were provided by the Japan National Institute of Biomedical Innovation, Health and Nutrition, Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). The cell lines were cultured in Roswell Park Memorial Institute medium (Life Technologies) supplemented with 10% fetal bovine serum (Gibco). Mycoplasma testing (MycoAlert Quick Mycoplasma Detection Kit, Lonza) was routinely conducted. Mycoplasma contamination was not detected during the study period.  RNA was extracted from the 1 x106 cells by QIAzol Lysis Reagent (QIAGEN). Concentrations of nucleic acids were measured by nanodrop (all experiments) and/or Qubit HS RNA-kit (only for RNA-seq). RNA quantity and quality were measured by Agilent 2100 Bioanalyzer (Agilent technologies). RNA integration values (RIN) >8 were considered to proceed to the further analysis. RNA-seq libraries were generated using Illumina TruSeq Stranded Total RNA kits. The resultant cDNA libraries were quantified via Qubit and assessed for quality via Bioanalyzer.
ENA first public
2024-08-01
INSDC center name
Jikei University School of Medicine
INSDC status
public
Submitter Id
E-MTAB-14212:MT2_RELA1
broker name
ArrayExpress
cell_line
MT-2
collection date
not collected
common name
human
genotype
RELA knockout
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-14212:MT2_RELA1
scientific_name
Homo sapiens