Homo sapiens
Protocols: Harvesting for both rt-QPCR and RNA sequencing was undertaken as follows. DM media was removed, and myotubes washed twice with PBS. The cells were harvested via scraping into the DPBS and cells were transferred to an Eppendorf tube and centrifuged as above and supernatant removed. The pellet was then re-suspended in 350 μl of Qiagen RLT lysis buffer and disrupted by gentle pipetting. Three hundred and fifty μl of 70% ethanol was added and RNA was extracted using the Qiagen mini-prep RNA extraction kit following manufacturer's instructions. Extracted RNA was stored in 30µl of RNA free purified water at -80°C until needed.  RNA was extracted using the Qiagen mini-prep RNA extraction kit RNA was depleted from samples using Ribo-ZeroTM rRNA removal kit, following the manufacturer's instructions. DNA free RNA was used for polyadenylation using the NEB Next poly(A) mRNA magnetic isolation module (New England Biolabs, Ipswich, UK).
isolate
not applicable
organism
Homo sapiens
organism
Homo sapiens
cell_line
AB1167
collection date
not collected
stimulus
none
scientific_name
Homo sapiens
common name
human
cell_type
skeletal muscle myotube
genotype
geographic location (country and/or sea)
not collected