Homo sapiens
Protocols: Cells were propagated to 70-90% confluence in T175cm2 flasks, incubated with cycloheximide (100 µg/ml, C7698, Sigma) 30min prior to harvest (scraping), washed (2x) with cold PBS buffer and resuspended in 400µl of 1x lysis buffer (#9803, CST) supplemented with protease/phosphatase inhibitor cocktail (#78442, Thermo Fisher) and 5mM of MgCl2 (HN03.1, ROTH). Cells were mechanically disrupted by vortexing (3x 10sec at max. output, 10min on ice in between), cleared by centrifugation (10min, 21.000rcf, 4°C) Hot phenol extraction of RNA SMARTer smRNA-Seq Kit for Illumina, Takara Bio
isolate
not applicable
disease
breast carcinoma
organism
Homo sapiens
organism
Homo sapiens
cell_line
MCF7
collection date
not collected
scientific_name
Homo sapiens
common name
human
organism part
mammary gland
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2023-06-21