Homo sapiens
Protocols: HT29 cells were seeded, were cultivated for 24 hours and were treated with BSA-conjugated LCFAs or an equal volume of DMSO as control for 48 h (n = 3). RNA was extracted using the miRNeasy Mini Kit (#74106, Qiagen). DNA was digested using RNase-free DNase (RNase-free DNase Set, #79256, Qiagen). RNA concentrations were assessed on a NanoDrop One Spectrometer. library preparation kit: Illumina Stranded mRNA library prep kit barcode kit/type: IDT for Illumina DNA/RNA adapters
disease
colorectal adenoma
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
scientific_name
Homo sapiens
common name
human
organism part
colon
developmental stage
adult
genotype
wild type genotype
isolate
not applicable
cell_line
HT-29
cell_type
colorectal cancer cell
geographic location (country and/or sea)
not collected