Mus musculus
Wild type C57BL/6J male mice were fed with low fat diet diet throughout the experiment including 4 weeks before AOM injection. Mice were injected with 10mg/kg body weight azoxymethane (AOM). One week after AOM injection, mice were exposed to DSS in drinking water for 7 days followed by 7 days of recovery (DSS-cycle). This DSS cycle was repeated two more times for a total of 3 times with 7 days between each cycle. Following this, epithelial (EpCAM+CD45-, EPI), immune (EpCAM-CD45+, IMM) or stromal cells (EpCAM-CD45-, double negative, DN) cells were sorted from the colon and processed for single cell transcriptomics analysis using protocol from 10X genomics.
organism
Mus musculus
collection date
2021-05-13
scientific_name
Mus musculus
common name
house mouse
geographic location (country and/or sea)
Sweden
ENA-LAST-UPDATE
2023-07-10