soil metagenome
The present work monitored, over a decade, the cumulative effect of repeated digestate application on soil microbial communities employing and in-situ approach, comparing the effect of the digested feedstock to the same undigested feedstock, and to others fertilization treatments. The study was carried out at the experimental field site EFELE, located in Rennes, France. The experiment was set up in 2012 and is based on a wheat-maize crop rotation. A mineral fertilizer (MIN), and three different OWP: cattle manure (CM), pig slurry (PS), and pig slurry anaerobic digestate (PS-DIG), were applied once every year since the beginning of the trial to the present. These treatments were compared to a control (0N) that had not received any organic or mineral N input. To monitor soil microbial community dynamics, from 2012 to 2022, soil samples were collected every year twelve months after the last fertilization treatment. For the molecular analyses performed in this study, were selected soil samples belonging to the beginning of the trial (2012), and wheat crop years (2013, 2015, 2017, 2019, and 2022). All the molecular analyses were conducted in 2022; we used high-throughput sequencing targeting 16S and 18S ribosomal RNA genes to describe the dynamics of microbial soil communities. Our results highlighted that over a decade of annual inputs of OWPs or mineral fertilizer, the soil chemical properties, the microbial biomass, and the composition and structure of soil microbial communities followed lasting modifications in all evaluated treatments. These changes were primarily explained by the time effect and, to a lesser extent, by the quality of the applied product. The temporal factor (year) exerted a strong influence on all measured parameters, potentially reflecting environmental, climatic, and seasonal effects that could mask the impact of digestate on soil microorganisms. Moreover, soil management practices appear to buffer the effects of different treatments, whether organic or mineral, on soil microbial quality indicators. The impact of digestate on soil microbial communities should be assessed considering associated agricultural practices.
elevation
34
sample volume or weight for DNA extraction
1
organism
soil metagenome
broad-scale environmental context
terrestrial environmental zone
collection date
2022
target gene
18S
geographic location (longitude)
-1.7956
environmental medium
agricultural soil
geographic location (latitude)
48.102
scientific_name
soil metagenome
pcr primers
F: 5'-CGATAACGAACGAGACCT-3'; R: 5'-ANCCATTCAATCGGTANT-3'
local environmental context
area of cropland
Treatment
PS
depth
0.2
project name
PRJEB71034
Block
1
multiplex identifiers
F: 5'-CGTACAGTCA-3'; R: 5'-CTAGATGCAC-3'
geographic location (country and/or sea)
France
ENA-LAST-UPDATE
2023-12-14