Lathyrus sativus
Protocols: The infected leaves were collected before inoculation and at 12, 48 and 72 hours after inoculation using liquid nitrogen. The samples were stored at -80 ºC until RNA extraction. Seedlings were grown in 0.5 L pots containing 250 cm3 of peat in a growth chamber at 20°C, with a 12-h light/12-h dark photoperiod until 14 days old. Fourteen-days old seedlings were inoculated with Erysiphe pisi isolate Ep-CO-01 using a settling tower to ensure uniform conidial deposition of 8 conidia/mm2. The samples 0 hai were not inoculated. Total RNA was isolated using the GeneJETTM Plant RNA Purification Mini Kit (ThermoScientificTM, Massachusetts, USA) according to the manufacturer's instructions.  The RNA library construction was carried out using a Stranded mRNA Library Preparation Kit (Roche/KAPA mRNA HyperPrep kit).
organism
Lathyrus sativus
organism
Lathyrus sativus
collection date
not collected
scientific_name
Lathyrus sativus
common name
chickling vetch
organism part
leaf
developmental stage
seedling development stage
genotype
Partial Susceptible
isolate
not applicable
cultivar
PI426882
age
14
geographic location (country and/or sea)
not collected
infect
Erysiphe pisi Ep-CO-01