Solanum lycopersicum
Protocols: tomato hairy root cultures were generated via the cotyledon hairy root transformation protocol. Hairy roots were subcultured on MS. After transgene root selection, roots were grown in liquid MS, and after 3 weeks treated with 100 um estradiol and harvested 24 hours after treatment tomato hairy root cultures in falcons grown on a shaker with 200 rpm tomato hairy root cultures treated with 100 um estradiol and harvested 24 hours after treatment Total RNA was extracted via the ReliaPrep RNA Miniprep Systems DNBSEQ stranded mRNA library processed through BGI
organism
Solanum lycopersicum
organism
Solanum lycopersicum
collection date
not collected
stimulus
estradiol
scientific_name
Solanum lycopersicum
common name
tomato
organism part
root
developmental stage
hairy root culture
genotype
wild type genotype
isolate
not applicable
cultivar
moneymaker
growth condition
liquid culture
time
24
age
3
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2024-01-05