Homo sapiens
Protocols: 1 × 106 iNKT cells were centrifuged and harvested in RLT buffer Total RNA (from 1 × 106 cells) was isolated with the RNeasy kit (Qiagen) and RNA quality was checked with the Agilent 2100 Bioanalyzer (Agilent Technologies). 0.5-1 μg were used to prepare libraries for RNA-seq with the Illumina TruSeq RNA Library Prep Kit v2 following the manufacturer's instructions.
isolate
not applicable
organism
Homo sapiens
organism
Homo sapiens
cell_line
PB5
collection date
not collected
scientific_name
Homo sapiens
common name
human
cell_type
mature NK T cell
geographic location (country and/or sea)
not collected