Medicago truncatula
Protocols: Fully expanded leaf tissue was sampled of Mtftb1/2 and control plants grown under LD (16h light/8h dark) for 2 weeks after 14 days of vernalization (Vernalized conditions) or plants grown for 60 days under LD (16h light/8h dark) without previous vernalization (non-vernalised conditions). Scarified seeds (seed surface scratched using fine sandpaper) were water imbibed and germinated at room temperature in a Petri dish in the dark. When radicles extended to a sufficient length (~10 mm), the seedlings were transferred into growth media (3 parts soil (Yates Professional Potting Mix, Auckland, New Zealand) and 1 part vermiculite) in controlled environment rooms. Plants were grown in a growth cabinet supplied with white light (TL5 28W low-pressure mercury discharge lamp, Philips, Auckland, New Zealand) ~90–110 µmol/m−2s−1) in 65% relative humidity at 25 °C or in a growth room, supplied with white light (~80–100 µmol/m−2s−1) in ~60% relative humidity at 18–23 °C. For vernalized conditioning, Medicago seeds were scarified and water imbibed overnight in the dark at room temperature before being placed at 4 °C in the dark for two weeks in a Petri dish. The seedlings were then planted into soil and put into the growth cabinet under the specified light conditions. Total RNA was extracted using the Spectrum Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO) Library construction and sequencing were performed at Otago Genomics Facility (OGF) (https://www.otago.ac.nz/genomics)
ENA first public
2024-08-01
External Id
SAMEA114610459
INSDC center name
University of Otago
INSDC last update
2023-11-14T14:16:23Z
INSDC status
public
Submitter Id
E-MTAB-13536:Mtftb1/2_1_NV
age
60
broker name
ArrayExpress
collection date
not collected
common name
barrel medic
cultivar
R108
developmental stage
a crucial time point when wild-type plants typically undergo the transition to flowering
genotype
mtftb1/2 mutant
geographic location (country and/or sea)
not collected
growth condition
NVLD
isolate
not applicable
organism part
leaf
sample name
E-MTAB-13536:Mtftb1/2_1_NV
scientific_name
Medicago truncatula