Mus musculus
Protocols: MC38 tumors harvested from immunocompetent C57BL/6 mice treated with different conditions Mechanic and enzymatic dissociation, and then CD45 positive sorting to obtain CD45+ single-cell suspensions to be sequenced CD45+ single-cell suspensions were stored at a concentration of 106 cells/mL and kept on ice until use. Single-cell suspensions were loaded onto a chromium single-cell chip (10X Genomics™) according to the manufacturer's instructions for coencapsulation with barcoded gel beads at a target capture rate of ~10,000 individual cells per sample. Captured mRNAs were barcoded during cDNA synthesis using the Chromium Next GEM Single-Cell 3' gel beads-in emulsion (GEM), Library and Gel Bead Kit v3.1 (10X Genomics™) according to the manufacturer's instructions. The resulting libraries were prepared in parallel in one single batch for each condition.
ENA-FIRST-PUBLIC
2023-10-15T00:25:51Z
ENA-LAST-UPDATE
2023-10-15T00:25:51Z
External Id
SAMEA114462869
INSDC center alias
Team INSERM U1030 \"Molecular radiotherapy and Therapeutic Innovations\"
INSDC center name
Team INSERM U1030 \"Molecular radiotherapy and Therapeutic Innovations\"
INSDC first public
2023-10-15T00:25:51Z
INSDC last update
2023-10-15T00:25:51Z
INSDC status
public
Submitter Id
E-MTAB-13389:Secondary tumor 2x2Gy+anti-PD-L1
age
10
broker name
ArrayExpress
collection date
not collected
common name
house mouse
geographic location (country and/or sea)
not collected
growth condition
MC-38 tumorigenic cells grown in mice
isolate
not applicable
organism part
secondary tumor
sample name
E-MTAB-13389:Secondary tumor 2x2Gy+anti-PD-L1
scientific_name
Mus musculus
strain
C57BL/6J
xenograft
MC-38 cell line