Gadus morhua
Protocols: The fish were sampled at 20 h or 48 h post injection. The fish were killed by a blow to the head. Liver samples were rapidly excised, snap frozen in liquid nitrogen and stored at -80°C for further processing. Liver total RNA extraction was performed from individual fish. A total of 32 individual liver RNA samples from groups of 20 h time point (Control, BPA (40 μmol/kg bw), MXC (40 μmol/kg bw) and EE2 (50 nmol/kg bw), n = 6 per group) and 48 h time point (Control, n = 3, EE2 = 5) were analyzed. RNA extraction was performed from each sample using TRI Reagent (Sigma) according to the manufacturer's protocols. The concentration and quality of total RNA was assessed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), and the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). RNA sequencing was performed at the Genomics Core Facility at the University of Bergen, as previously described (Yadetie et al., 2018), according to Illumina TruSeq® Stranded mRNA Sample Preparation Guide on Illumina HiSeq 4000 (Illumina, Inc., San Diego, CA, USA).
ENA first public
2024-01-01
External Id
SAMEA112641195
INSDC center name
Department of Biological Sciences University of Bergen, Bergen, Norway
INSDC last update
2023-02-13T14:57:14Z
INSDC status
public
Submitter Id
E-MTAB-12606:I7-2
age
1.5
broker name
ArrayExpress
common name
Atlantic cod
developmental stage
juvenile stage
disease
normal
genotype
wild type genotype
isolate
not applicable
organism part
liver
sample name
E-MTAB-12606:I7-2
scientific_name
Gadus morhua
sex
male