Homo sapiens
Protocols: Endometrial biopsies were collected with a catheter (Rampipella, RI.MOS., Mirandola, Italy) from the uterine cavity between days 21 and 23 of the menstrual cycle under sterile conditions prior to the IVF procedure.  Total RNA was isolated from endometrial tissue using the RNeasy Mini Kit reagent kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions (https://www.qiagen.com). The purity of the isolated RNA was assessed using a NanoDrop 2000c spectrophotometer (Nanodrop Technologies, Wilmington, Delaware, USA). Integrity was determined on an Agilent 2100 bioanalyzer using an RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, California, USA).  The library was prepared using the TruSeq RNA reagent kit (Illumina, San Diego, California, USA) according to the manufacturer's instructions. This protocol was used to convert the whole RNA into a fragment library for next-generation sequencing. After removal of ribosomal RNA using the Ribo-Zero rRNA approach (Illumina, San Diego, California, USA), the remaining RNA was purified, fragmented and the first strand cDNA (complementary DNA) was synthesized using reverse transcriptase and random oligonucleotide primers. This was followed by the synthesis of a second cDNA chain with deoxyuridine triphosphate nucleotides. The double-stranded DNA, which was terminated with blunt ends, was further adenylated at the 3'end in the next step. Sequences with molecular barcodes and adapters were added to the ends of the double-stranded cDNA to bind to the surface of the flow cell. Fragments with ligated adaptors at both ends were selectively enriched by 15 cycles of PCR.
treatment
none
disease
polycystic ovary syndrome
individual
EN039
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
female
scientific_name
Homo sapiens
common name
human
organism part
endometrium
developmental stage
adult
isolate
not applicable
age
39
geographic location (country and/or sea)
not collected