Homo sapiens
Protocols: Cells were collected after 72 hours from transfection. MSTO-211H were cultured in RPMI GlutaMax medium added with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. Cells were grown at 37°C, 5% CO2. cells were reverse-transfected with siRNA against linc00941 or Negative Control siRNA at a final concentration of 20 nM. Total RNA was purified with Maxwell® RSC simplyRNA Cells (Promega). RNAseq libraries were prepared by Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit starting from 500 ng of RNA. RNA quality was assessed by Bioanalyzer RNA 6000 nano kit.
ENA first public
2024-09-30
External Id
SAMEA114469634
INSDC center name
Laboratory of Translational Research, Azienda USL – IRCCS di Reggio Emilia, Reggio Emilia, Italy
INSDC last update
2023-10-08T17:02:25Z
INSDC status
public
Submitter Id
E-MTAB-13400:mock-2307
broker name
ArrayExpress
cell_line
MSTO-211H
cell_type
mesothelial cell
collection date
not collected
common name
human
developmental stage
adult
disease
malignant pleural mesothelioma
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lung
replicate
3
sample name
E-MTAB-13400:mock-2307
scientific_name
Homo sapiens