Mus musculus
Protocols: The dorsal telencephalon of E12.5 mouse embryos were collected in 1.5ml Eppendorff tubes and was shock frozen on dry ice and stored at -80C.  Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer's instructions. RNA was eluated in 14 μl of RNAse free water.  Libraries were prepared from 1 μg of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) and the Poly-A mRNA magnetic isolation module (NEB #E7490)
ENA first public
2024-01-01
INSDC center name
University of Edinburgh Centre for Discovery Brain Sciences
INSDC status
public
Submitter Id
E-MTAB-14015:mut212
age
12.5
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
E12.5
genotype
mutant genotype
geographic location (country and/or sea)
not collected
individual
mut212
isolate
not applicable
organism part
dorsal telencephalon
sample name
E-MTAB-14015:mut212
scientific_name
Mus musculus
sex
unknown
strain
C57BL6