Homo sapiens
Protocols: Genomic DNA was extracted from the whole blood of children using a Blood DNA Extraction Kit (Tiangen Biotech, China), then treated with EpiTect Fast DNA Bisulfite Kit (QIAGEN, Germany) for bisulfite conversion. The converted DNA was PCR amplified with primer sequences designed to cover the CpGs in two promoter regions: each region included near 1,000 bp centering around the transcription start sites (TSSs) of SLC22A18. The PCR products were purified and used for DNA library preparation. According to the manufacturer's protocol, the library was prepared with the KAPA HTP Library Preparation Kit (KAPA Biosystems, USA).
isolate
not applicable
disease
normal
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
scientific_name
Homo sapiens
common name
human
organism part
blood
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2024-01-30