Ceratina calcarata
One biological sample of fresh-frozen brain tissue from six adult Ceratina calcarata dwarf-eldest daughters (DED) was dissociated with S2 Genomics Singulator 100 system. Samples were loaded into 10X Genomics Gel-beads-in-emulsion wells on Chromium Chips following their protocol for Single Cell 3-prime Reagent Kits (v3.1). After dissolving the gel beads, nuclei were lysed, and mRNA was reverse transcribed to cDNA. Using PCR, the cDNA was then amplified to create libraries. Libraries were checked for quality using the Agilent Bioanalyzer High Sensitivity chip. Paired-end reads 1 and 2 and sample index reads were then sequenced on a flow cell lane (L003, L004, L005, L006, L007 or L008) with an Illumina Novaseq X to create base call files. Base call files were converted to fastq files for read 1 (R1, 28 bp) and read 2(R2, 90 bp) using Illumina BCL Convert (v. 4.1.5).