Homo sapiens
Protocols: First, frozen patient peritoneal cells were thawed and counted.  Next, Natural Killer (NK) cells were purified using the negative selection kit from Miltenyii (130-092-657), according to the manufactures protocol. In order to increase the purity, NK cells underwent a second round of purification using the positive selection kit from Miltenyii (130-050-401), according to the manufactures protocol. The purity was determined via FACS and microscopy and the NK cells were then lysed in 1 ml Trizol. RNA was isolated using Trizol/TriFast (30-2010), according to the manufacturers' protocol.  DNA was removed using the DNA-freeâ„¢ DNA Removal Kit (Ambion, AM1906), according to the manufacturers' protocol. Illumina Truseq mRNA stranded, according to manufacturers' instructions