bat gut metagenome
host
Myotis escalerai
isolation_source
Bat guano
collection_date
2022-06-02/2022-08-16
geo_loc_name
Spain: Lugo, Murcia and Salamanca
lat_lon
not provided
samp_mat_process
Pool of 9 fecal samples homogenized in a Precellys Evolution tissue homogenizer (Bertin) in 2 mL tubes with 1.4 mm ceramic beads, adding 1 volume of 1x PBS to obtain a final volume of 1.5 mL. Homogenization consisted of 3 cycles of 30 sec at 6500 rpm, with a 10 sec pause between cycles. Homogenates were centrifuged in two rounds at 20,000 g for 3 min at 4 C. Supernatants were transferred to new tubes and filtered using Minisart cellulose acetate syringe filters with a 1.2 m pore size (Sartorius). The filtrate was transferred to ultra-clean 2 mL tubes and 280 L were collected for nucleic acid extraction using the QIAamp Viral RNA mini kit (Qiagen). The extract was eluted in a final volume of 40 L and stored at -80 C.
source_material_id
P29
BioSampleModel
Metagenome or environmental