Homo sapiens
isolate
GIAB HG008
age
61
biomaterial_provider
Massachusetts General Hospital (MGH)
collection_date
2020-12-30
geo_loc_name
USA
sex
female
tissue
Pancreatic Ductal Adenocarcinoma Cell Line (PDAC)
disease
pancreatic ductal adenocarcinoma
sample_type
cell culture
super_population_code
EUR
super_population_description
European
is_tumor
yes
isolation_source
pancreatic ductal adenocarcinoma
cell_line
HG008-T
cell_subtype
epithelial-like
cell_type
pancreatic ductal adenocarcinoma
disease_stage
tumor stage ypT2 N1, histological grade G2 (Moderately differentiated)
treatment
neoadjuvant therapy, tumor regression grade 2 (Minimal response: Residual cancer outgrown by fibrosis)
passage_history
The individual (HG008) was explicitly consented for tissue collection and public genomic data sharing following a Massachusetts General Hospital (MGH) IRB-approved protocol. The Liss laboratory at MGH established a PDAC tumor cell line (HG008-T) from the resected PDAC tumor. PDAC tumors are stromal-rich, with cancer cells often only composing 10% of the tumor. The cell line was developed starting with approximately 50 mg of tumor tissue being enzymatically dissociated to culture tumor cells. While PDAC cell cultures often contain substantial fibroblasts, all cells appeared to be tumor cells even at passage 2 for this cell line. Cells were cultured for the first 20 passages using "Rich" media composed of DMEM/F12 with 20% Fetal Bovine Serum (FBS) Nicotinamide (0.01 M), cholera toxin (8.4 ng/ml), Epidermal Growth Factor (EGF; 10 ng/ml)., Hepatocyte Growth Factor (HGF: 25 ng/ml), human recombinant insulin (10 ug/ml), human recombinant transferrin (5.5 ug/ml), and selenious acid (6.7 ng/ml). Morphologically pure cancer cell cultures, free of tumor-associated fibroblasts, were considered cell lines after growing for at least 20 passages. Passages 21-24 were grown the same media as prior passages however without EGF. A large homogenous batch (batch 0823p23) of tumor cells was cultured and at passage 23, approximately 80 million tumor cells were aliquoted, pelleted and snap-frozen for distribution and measurement of non-viable cells.
passage_method
Cells were enzymatically removed from the culture surface using 0.25% trypsin and split by diluting no more than 1:3 for the first 20 passages. Passages 21-24 were diluted 1:3.
passage_number
Liss_0823p23
BioSampleModel
Human