EMOBON_RFormosa_So_1
marine sediment metagenome
ENA first public
2024-11-11
INSDC center name
European Marine Biological Resource Centre
INSDC status
public
Submitter Id
EMOBON00092
broad-scale environmental context
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
chlorophyll
21.69
chlorophyll method
Fluorometer 10-AU
collection date
2021-08-05
depth
0
dissolved oxygen
271.875
dissolved oxygen method
YSI ProSolo Digital
dna concentration
9.05
elevation
0
environmental medium
lagoon [ENVO_00000038]; sediment [ENVO:00002007]
geographic location (country and/or sea)
Portugal
geographic location (latitude)
37.005639
geographic location (locality)
Ria Formosa
geographic location (longitude)
-7.96925
geographic location (ocean)
Atlantic Ocean
geographic location (region and locality)
North Atlantic Ocean
library reads sequenced
61701779
local environmental context
lagoon [ENVO_00000038];shallow_marine_sediment [ENVO:03000034]
mock_pr
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
nucleic acid amplification
metag
nucleic acid extraction
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
obs_id
RFormosa
pH
7.9
pcr conditions
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr primers
metag
ph method
Thermo scientific orion star A221
pressure
0.998766
pressure method
YSI ProSolo Digital
project name
EMOBON
redox potential
-101.7
redox potential method
Thermo scientific orion star A221
relevant standard operating procedures
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
salinity
38.03
salinity method
YSI ProSolo Digital
sample collection device
PVC core D50mm
sample material processing
SoSOP 1
sample name
EMOBON00092
sample storage location
Ed7, room 1.10, freezer #300387-118
sample storage temperature
-80
scientific_name
marine sediment metagenome
sea surface temperature
26.3
sea surface temperature method
Thermo scientific orion star A221
sediment temperature
24
sediment temperature method
Thermo scientific orion star A221
sequence quality check
manual
sequence_batch
Batch1
sequence_batch_rundate
Batch1_220712
sequencing method
Illumina NovaSeq 6000
source material identifiers
EMOBON_RFormosa_So_210805_micro_1
status
sample
target gene
metag
target subfragment
metag
tidal stage
low
tidal stage information
low_tide
total depth of water column
0