EMOBON_BPNS_So_5
marine sediment metagenome
ENA first public
2024-11-11
INSDC center name
European Marine Biological Resource Centre
INSDC status
public
Submitter Id
EMOBON00084
broad-scale environmental context
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
collection date
2021-07-26
depth
22.94
dna concentration
13.9
elevation
-22.94
environmental medium
sediment [ENVO:00002007]
geographic location (country and/or sea)
Belgium
geographic location (latitude)
51.433331
geographic location (locality)
Belgian part of the North Sea
geographic location (longitude)
2.808331
geographic location (ocean)
North Atlantic Ocean
geographic location (region and locality)
North Sea
library reads sequenced
47913214.0
local environmental context
shallow_marine_sediment_[ENVO:03000034]
mock_pr
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
nucleic acid amplification
metag
nucleic acid extraction
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
obs_id
BPNS
pcr conditions
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr primers
metag
project name
EMOBON
relevant standard operating procedures
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
run
220712_JARVIS_HMNJKDSX3
sample collection device
Van Veen Grab
sample collection method
Van Veen Grab
sample material processing
SoSOP3
sample name
EMOBON00084
sample storage location
MSO LifeWatch Lab -80 Freezer
sample storage temperature
-80
scientific_name
marine sediment metagenome
sea surface temperature method
no CTD taken
sediment temperature method
no CTD taken
sequence quality check
manual
sequence_batch
Batch1
sequence_batch_rundate
Batch1_220712
sequencing method
Illumina NovaSeq 6000
source material identifiers
EMOBON_BPNS_So_210726_micro_1
status
sample
target gene
metag
target subfragment
metag
tidal stage
high
tidal stage information
flood_tide
total depth of water column
26