EMOBON_HCMR-1_Wa_21
metagenome
INSDC center name
European Marine Biological Resource Centre
Submitter Id
EMOBON00076
amount or size of sample collected
30
broad-scale environmental context
Control
collection date
2021-09-17
depth
0
dna concentration
878
environmental medium
MilliQ water [ENVO:00003065]
geographic location (country and/or sea)
missing: control sample
geographic location (latitude)
missing: control sample
geographic location (locality)
missing: control sample
geographic location (longitude)
missing: control sample
geographic location (ocean)
missing: control samplen
geographic location (region and locality)
missing: control samplea
library reads sequenced
84837438
local environmental context
Negative
mock_eu
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_pr
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
nucleic acid amplification
metag
nucleic acid extraction
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
obs_id
HCMR-1
pcr conditions
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:11
pcr primers
metag
project name
EMOBON
relevant standard operating procedures
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
sample collection device
Jerrycan
sample material processing
WaSOP 1
sample name
EMOBON00076
sample storage location
Basement, New chest freezer -80C, Left side, Carton box
sample storage temperature
-80
scientific_name
metagenome
sequence quality check
manual
sequence_batch
Batch1
sequence_batch_rundate
Batch1_220622
sequencing method
Illumina NovaSeq 6000
size-fraction lower threshold
3
size-fraction upper threshold
200
source material identifiers
EMOBON_HCMR-1_Wa_210917_3um_blank
status
blank
target gene
metag
target subfragment
metag
tidal stage information
no_tide
total depth of water column
21