DRS_W303_+_thiolutin_20min_25°C_repA_1_sc. yeast cells, YPDA, WT W303, grow in 25°C with thiolutin added to a final concentration of 4 ug/ml for 20 minutes, biological replicate A, technical replicate 1. Mixed with 2–5 ug of cap-enriched Arabidopsis thaliana atcfi59-1/atcfi68-3, biological replicate 3, technical replicate 2 mRNA and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer's protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.
Saccharomyces cerevisiae
INSDC center name
INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW
Submitter Id
DRS_W303_+_thiolutin_20min_25°C_repA_1_sc
collection date
2022
common name
brewer's yeast
geographic location (country and/or sea)
Poland
sample name
DRS_W303_+_thiolutin_20min_25°C_repA_1_sc
scientific_name
Saccharomyces cerevisiae