EMOBON_OOB_So_1
marine sediment metagenome
ENA first public
2024-11-11
INSDC center name
European Marine Biological Resource Centre
INSDC status
public
Submitter Id
EMOBON00001
broad-scale environmental context
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
collection date
2021-06-08
depth
27
dna concentration
1.7
elevation
-27
environmental medium
sediment [ENVO:00002007]
geographic location (country and/or sea)
France
geographic location (latitude)
42.484167
geographic location (locality)
Bay of Banyuls-sur-Mer
geographic location (longitude)
3.135278
geographic location (ocean)
Mediterranean Sea - Western Basin
geographic location (region and locality)
Gulf of Lion
library reads sequenced
58079642
local environmental context
shallow_marine_sediment_[ENVO:03000034]
mock_eu
DDC_AAABOSDA_1_HWLTKDRXY.UDI213
mock_pr
DDC_AAAAOSDA_1_HWLTKDRXY.UDI212
nucleic acid amplification
metag
nucleic acid extraction
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
obs_id
OOB
pH
7.962
pcr conditions
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr primers
metag
ph method
Consort multimeter C3016
project name
EMOBON
relevant standard operating procedures
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
salinity
37.85
salinity method
Seabird SBE 911plus CTD
sample collection device
PVC sediment corer
sample material processing
SoSOP 2
sample name
EMOBON00001
sample storage location
room 213, freezer LECOB
sample storage temperature
-80
scientific_name
marine sediment metagenome
sea surface temperature
18.75
sea surface temperature method
Seabird SBE 911plus CTD
sediment temperature
18.66
sediment temperature method
Consort temperature compensator (ST10) PT1000
sequence quality check
manual
sequence_batch
Batch1
sequence_batch_rundate
Batch1_220223
sequencing method
Illumina NovaSeq 6000
source material identifiers
EMOBON_OOB_So_210608_micro_1
status
sample
target gene
metag
target subfragment
metag
tidal stage information
no_tide
total depth of water column
27