EMOBON_IUIEilat_Wa_6
marine plankton metagenome
ENA first public
2024-11-11
INSDC center name
European Marine Biological Resource Centre
INSDC status
public
Submitter Id
EMOBON00083
amount or size of sample collected
10
broad-scale environmental context
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
chlorophyll
0.094167
chlorophyll method
Trilogy fluorometer - Module: CHL-A NA model # 7200-046
collection date
2021-08-29
depth
2
dna concentration
1.92
environmental medium
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
geographic location (country and/or sea)
Israel
geographic location (latitude)
29.5
geographic location (locality)
Gulf of Eilat
geographic location (longitude)
34.916667
geographic location (ocean)
Indian Ocean
geographic location (region and locality)
Gulf of Eilat
library reads sequenced
83595057
local environmental context
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
mock_eu
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_pr
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
nucleic acid amplification
metag
nucleic acid extraction
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
obs_id
IUIEilat
pcr conditions
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr primers
metag
project name
EMOBON
relevant standard operating procedures
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
sample collection device
Hand pump
sample material processing
WaSOP 1
sample name
EMOBON00083
sample storage location
Eppendorf F570 -80 freezer
sample storage temperature
-80
scientific_name
marine plankton metagenome
sea surface temperature
27.4
sea surface temperature method
Thermistor - Campbell Scientific 108
sequence quality check
manual
sequence_batch
Batch1
sequence_batch_rundate
Batch1_220622
sequencing method
Illumina NovaSeq 6000
size-fraction lower threshold
3
size-fraction upper threshold
200
source material identifiers
EMOBON_IUIEilat1_Wa_210829_3um_2
status
sample
subsurface salinity
40.7649
subsurface salinity method
MS-315e Salinometer
target gene
metag
target subfragment
metag
tidal stage information
no_tide
total depth of water column
700