EMOBON_NRMCB_So_1
marine sediment metagenome
ENA first public
2024-11-11
INSDC center name
European Marine Biological Resource Centre
INSDC status
public
Submitter Id
EMOBON00094
broad-scale environmental context
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
collection date
2021-06-21
depth
75
dissolved oxygen
4.741
dissolved oxygen method
SBE43
dna concentration
16.7
elevation
-75
environmental medium
sediment [ENVO:00002007]
geographic location (country and/or sea)
Italy
geographic location (latitude)
40.800139
geographic location (locality)
Naples Gulf
geographic location (longitude)
14.25
geographic location (ocean)
Mediterranean Sea - Western Basin
geographic location (region and locality)
Tyrrhenian Sea
library reads sequenced
98315027
local environmental context
shallow_marine_sediment_[ENVO:03000034]
mock_eu
DDC_AAABOSDA_1_HWLTKDRXY.UDI213
mock_pr
DDC_AAAAOSDA_1_HWLTKDRXY.UDI212
nucleic acid amplification
metag
nucleic acid extraction
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
obs_id
NRMCB
pcr conditions
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:11
pcr primers
metag
pigments
0.5523
pigments method
this is not pigments but fluorescense. chl Turner Design Cyclops 7C)
project name
EMOBON
relevant standard operating procedures
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
salinity
37.5864
salinity method
CTD SBE 19plusV2
sample collection device
sediment corer
sample material processing
So SOP1
sample name
EMOBON00094
sample storage location
freezer #1 BEOM; upper compartment; rack #8 d III (see scheme in technical support EMI)
sample storage temperature
-80
scientific_name
marine sediment metagenome
sea surface temperature
25.0495
sea surface temperature method
CTD SBE 19plusV2
sediment temperature
14.999
sediment temperature method
CTD SBE 19plusV2
sequence quality check
manual
sequence_batch
Batch1
sequence_batch_rundate
Batch1_220223
sequencing method
Illumina NovaSeq 6000
source material identifiers
EMOBON_NRMCB_So_210621_micro_1
status
sample
target gene
metag
target subfragment
metag
tidal stage information
no_tide
total depth of water column
75