DRS_Mex67_AA_20_min_25°C_repB_3_sc. yeast cells, minimal media that lacked uracil, with 2% raffinose and 2% galactose, Mex67-AA, grow in 25°C, rapamycin added to a final concentration of 1 μg/ml, 20min of treatment, biological replicate B, technical replicate 3. 100–150 ng of yeast oligo(dT)-enriched RNA mixed with 2–5 ug of cap-enriched Arabidopsis thaliana atcfi25a-1/atcfi25b-2, biological replicate 2, technical rep 2 mRNA and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer's protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.
Saccharomyces cerevisiae
INSDC center name
INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW
Submitter Id
DRS_Mex67_AA_20_min_25°C_repB_3_sc
collection date
2022
common name
brewer's yeast
geographic location (country and/or sea)
Poland
sample name
DRS_Mex67_AA_20_min_25°C_repB_3_sc
scientific_name
Saccharomyces cerevisiae