DRS_Mex67_AA_0_min_25°C_control_(-_rapamycin)_repB_3_sc. yeast cells, minimal media that lacked uracil,  with 2% raffinose and 2% galactose, WT W303,  grow in 25°C,  without rapamycin added to a final concentration of 1 μg/ml,  0min of treatment,  biological replicate B,  technical replicate 3 Mixed with 2–5 ug of cap-enriched Arabidopsis thaliana SALK_074069(ABI3MTA), biological replicate 3 mRNA and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer's protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.
Saccharomyces cerevisiae
INSDC center name
INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW
Submitter Id
DRS_Mex67_AA_0_min_25°C_control_(-_rapamycin)_repB_3_sc
collection date
2022
common name
brewer's yeast
geographic location (country and/or sea)
Poland
sample name
DRS_Mex67_AA_0_min_25°C_control_(-_rapamycin)_repB_3_sc
scientific_name
Saccharomyces cerevisiae