EMOBON_EMT21_Wa_6
marine plankton metagenome
ENA first public
2024-11-11
INSDC center name
European Marine Biological Resource Centre
INSDC status
public
Submitter Id
EMOBON00033
ammonium
0.13
ammonium method
Segmented flow analysis
amount or size of sample collected
5
broad-scale environmental context
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
chlorophyll
0.549
chlorophyll method
Chlorophyll extraction
collection date
2021-08-25
conductivity
44.63
conductivity method
SBE_37ODO
depth
1
dissolved oxygen
7.439
dissolved oxygen method
SBE_37ODO
dna concentration
6.86
environmental medium
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
geographic location (country and/or sea)
Spain
geographic location (latitude)
42.201944
geographic location (locality)
Vigo Seamount
geographic location (longitude)
-8.7985
geographic location (ocean)
Atlantic Ocean
geographic location (region and locality)
North Atlantic Ocean
library reads sequenced
48908124
local environmental context
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
mock_pr
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
nitrate
0.23
nitrate method
Segmented flow analysis
nitrite
0.07
nitrite method
Segmented flow analysis
nucleic acid amplification
metag
nucleic acid extraction
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
obs_id
EMT21
pH
8.21
pcr conditions
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr primers
metag
ph method
Hach probe
phosphate
0.13
phosphate method
Segmented flow analysis
project name
EMOBON
relevant standard operating procedures
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
salinity
35.08
salinity method
Hach probe
sample collection device
Niskin
sample material processing
WaSOP 1
sample name
EMOBON00033
sample storage location
cryopreservation room
sample storage temperature
-80
scientific_name
marine plankton metagenome
sea subsurface temperature
16.21
sea subsurface temperature method
SBE_37ODO
sea surface temperature
17
sea surface temperature method
Hach probe
sequence quality check
manual
sequence_batch
Batch1
sequence_batch_rundate
Batch1_220712
sequencing method
Illumina NovaSeq 6000
size-fraction lower threshold
0.2
size-fraction upper threshold
3
source material identifiers
EMOBON_EMT21_Wa_210825_0.2um_1
status
sample
subsurface salinity
35.46
subsurface salinity method
SBE_37ODO
target gene
metag
target subfragment
metag
tidal stage
low
tidal stage information
ebb_tide
total depth of water column
5