EMOBON_ESC68N_Wa_2
marine plankton metagenome
ENA first public
2024-11-11
INSDC center name
European Marine Biological Resource Centre
INSDC status
public
Submitter Id
EMOBON00004
amount or size of sample collected
10
broad-scale environmental context
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
chlorophyll
0.821
chlorophyll method
photometric measurement
collection date
2021-08-30
depth
2
dna concentration
5.05
environmental medium
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
geographic location (country and/or sea)
Norway
geographic location (latitude)
68.925895
geographic location (locality)
Norwegian part of the Norwegian Sea
geographic location (longitude)
17.125619
geographic location (ocean)
Arctic Ocean
geographic location (region and locality)
Norwegian Sea
library reads sequenced
81370157
local environmental context
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049];fjord [ENVO_00000039]
mock_eu
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_pr
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
nucleic acid amplification
metag
nucleic acid extraction
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
obs_id
ESC68N
pcr conditions
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr primers
metag
project name
EMOBON
relevant standard operating procedures
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
salinity
32.5
salinity method
CTD-system
sample collection device
Niskin
sample material processing
WaSOP1
sample name
EMOBON00004
sample storage location
eDNA freezer bag
sample storage temperature
-80
scientific_name
marine plankton metagenome
sea subsurface temperature
11.67
sea subsurface temperature method
CTD-system
sea surface temperature
11.69
sea surface temperature method
CTD-system
sequence quality check
manual
sequence_batch
Batch1
sequence_batch_rundate
Batch1_220622
sequencing method
Illumina NovaSeq 6000
size-fraction lower threshold
3
size-fraction upper threshold
200
source material identifiers
EMOBON_ESC68N_Wa_210830_3um_2
status
sample
subsurface salinity
32.5
subsurface salinity method
CTD-system
target gene
metag
target subfragment
metag
tidal stage information
no_tide