EMOBON_RFormosa_Wa_1
marine plankton metagenome
ENA first public
2024-11-11
INSDC center name
European Marine Biological Resource Centre
INSDC status
public
Submitter Id
EMOBON00041
amount or size of sample collected
5
broad-scale environmental context
marine biome [ENVO:00000447]
chlorophyll
21.69
chlorophyll method
Fluorometer 10-AU
collection date
2021-08-05
depth
0.2
dissolved oxygen
271.875
dissolved oxygen method
YSI ProSolo Digital
dna concentration
22.2
environmental medium
lagoon [ENVO_00000038];marine water body [ENVO:00001999]; surface water [ENVO:00002042]
geographic location (country and/or sea)
Portugal
geographic location (latitude)
37.005639
geographic location (locality)
Ria Formosa
geographic location (longitude)
-7.96925
geographic location (ocean)
Atlantic Ocean
geographic location (region and locality)
North Atlantic Ocean
library reads sequenced
89249928
local environmental context
lagoon [ENVO_00000038];marine water body [ENVO:00001999]
mock_eu
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_pr
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
nucleic acid amplification
metag
nucleic acid extraction
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
obs_id
RFormosa
pH
8.242
pcr conditions
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr primers
metag
ph method
Thermo scientific orion star A221
pressure
0.998766
pressure method
YSI ProSolo Digital
project name
EMOBON
relevant standard operating procedures
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
salinity
38.03
salinity method
YSI ProSolo Digital
sample collection device
bucket
sample material processing
WaSOP 1
sample name
EMOBON00041
sample storage location
Ed7, room 1.10, freezer #300387-118
sample storage temperature
-80
scientific_name
marine plankton metagenome
sea subsurface temperature
26.3
sea subsurface temperature method
Thermo scientific orion star A221
sea surface temperature
26.3
sea surface temperature method
Thermo scientific orion star A221
sequence quality check
manual
sequence_batch
Batch1
sequence_batch_rundate
Batch1_220622
sequencing method
Illumina NovaSeq 6000
size-fraction lower threshold
3
size-fraction upper threshold
200
source material identifiers
EMOBON_RFormosa_Wa_210805_3um_1
status
sample
subsurface salinity
38.03
subsurface salinity method
YSI ProSolo Digital
target gene
metag
target subfragment
metag
tidal stage
low
tidal stage information
low_tide
total depth of water column
1