BPP7
human gut metagenome
host
Homo sapiens
isolation_source
human feces
collection_date
2022
geo_loc_name
USA: Kannapolis, North Carolina
lat_lon
35.4874 N 80.6217 W
samp_collect_device
A stool collection kit with instructions was given to each participant with instructions to collect the day before visit 3. One Day Prior to Visit 3: Subjects consumed the same dinner that they ate the evening prior to Visit #1, and collected a small swab sample of stool in a container provided by the lab. Sample was stored at room temperature, and returned to the lab within 24 hours. Samples were stored at -20C until processing.
samp_mat_process
DNA was extracted from sixteen human fecal samples and two control samples (water and RNA-later) using the Zymo Quick-DNA™ Fecal/Soil Microbe MicroPrep Kit, according to kit instructions. The protocol was performed in a BSL2 hood, and all biosafety work was approved by the University of Maine Institutional Biosafety Committee (protocol SI-092220). DNA extract concentration was determined using a NanoDrop® ND-1000 UV-Vis Spectrophotometer (Thermo Scientific).
samp_size
5 g
DNA_extract_ng/ul
15.5
index
TTAGACCATG
index2
TCCTAGGAAG
SFN_nitrile_trtA
5.01
SFN_nitrile_trtB
8.53
First_treatment
B
Percent_GLR_conversion_trtB
23.53138754
Percent_GLR_conversion_trtA
64.65087044
E.coliVL8_Bglucosidase
3.47E+30
E.coli_Bglucosidase3
0
En.casseliflavus_Bglucosidase4
11.63164924
En.casseliflavus_Bglucosidase44
0
L.agilisR16_msr3
0
Total_others
11.63164924
Btheta2156
14269.43848
Btheta2157
1649.891113
Btheta2158
44511.40104
Btheta2159
6998.728027
Btheta2160
80.65299733
Btheta_operon
67510.11166
BioSampleModel
Metagenome or environmental