BPP14
human gut metagenome
host
Homo sapiens
isolation_source
human feces
collection_date
2022
geo_loc_name
USA: Kannapolis, North Carolina
lat_lon
35.4874 N 80.6217 W
samp_collect_device
A stool collection kit with instructions was given to each participant with instructions to collect the day before visit 3. One Day Prior to Visit 3: Subjects consumed the same dinner that they ate the evening prior to Visit #1, and collected a small swab sample of stool in a container provided by the lab. Sample was stored at room temperature, and returned to the lab within 24 hours. Samples were stored at -20C until processing.
samp_mat_process
DNA was extracted from sixteen human fecal samples and two control samples (water and RNA-later) using the Zymo Quick-DNA™ Fecal/Soil Microbe MicroPrep Kit, according to kit instructions. The protocol was performed in a BSL2 hood, and all biosafety work was approved by the University of Maine Institutional Biosafety Committee (protocol SI-092220). DNA extract concentration was determined using a NanoDrop® ND-1000 UV-Vis Spectrophotometer (Thermo Scientific).
samp_size
5 g
DNA_extract_ng/ul
16
index
AAGAGAGTCT
index2
TACGAATCTT
SFN_nitrile_trtA
7.52
SFN_nitrile_trtB
10.36
First_treatment
A
Percent_GLR_conversion_trtB
18.56125708
Percent_GLR_conversion_trtA
28.11729407
E.coliVL8_Bglucosidase
9.87E+27
E.coli_Bglucosidase3
832.2099457
En.casseliflavus_Bglucosidase4
9.148741628
En.casseliflavus_Bglucosidase44
0.720478823
L.agilisR16_msr3
0
Total_others
842.0791662
Btheta2156
27535.21029
Btheta2157
59.24250476
Btheta2158
3872.779378
Btheta2159
3598.187785
Btheta2160
827.4692891
Btheta_operon
35892.88925
BioSampleModel
Metagenome or environmental