UKF_CDDP_RNA
Homo sapiens
isolate
human penile carcinoma
age
64
biomaterial_provider
Dr. Senckenberg Institute of Pathology, Frankfurt am Main; Dr. Petra Joh Forschungshaus; Institute for Virology Frankfurt am Main
sex
male
tissue
penile sqamous cell carcinoma
biosample_accession
SAMN6
library_id
UKF_CDDP_RNA
title
UKF_CDDP_RNA
library_strategy
AMPLICON
library_source
GENOMIC
library_selection
cDNA
library_layout
paired
platform
ION_TORRENT
instrument_model
Ion Torrent S5
design_description
All laboratory work was performed according to manufacturers instructions. Representative tumor material of the primary tumor was macrodissected. The cell lines were formalin fixed and paraffin embedded and taken as whole slides. The purification of DNA and RNA from FFPE tissue samples was performed using the Maxwell RSC Instrument (Promega Corporation, Madison, Wisconsin, USA) with the Maxwell RSC FFPE Plus RNA Kit (Promega Corporation, Madison, Wisconsin, USA) and QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany), respectively. The concentration of DNA and RNA was measured with the Qubit 4 Fluorometer (Invitrogen, Thermo Fisher, Waltham, Massachusetts, USA). For the library preparation 20 ng for the OncomineTM Comprehensive Assay v3 (Thermo Fisher, Waltham, Massachusetts, USA) were used. A 540 chip was applied.
filetype
bam
filename
UKF_CDDP_RNA.bam
BioSampleModel
Human