BACMIX_minion_v1_9_20230623_zymo_magbead_NZ2
gut metagenome
chimera check software
minitax
broad-scale environmental context
not applicable
host scientific name
not applicable
sequence quality check
LongQC
collection date
not collected
target gene
16S rRNA
host family relationship
not applicable
geographic location (longitude)
not applicable
host subject id
not applicable
host subspecific genetic lineage
not applicable
host common name
not applicable
host sex
not applicable
temperature
20
nucleic acid extraction
ZymoBIOMICS 96 MagBead DNA Kit, Zymo
nucleic acid amplification
LongAmp Taq 2X Master Mix
geographic location (country and/or sea)
not applicable
library reads sequenced
not provided
sample storage temperature
-80
adapters
16SFw 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'; 16S Rev 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'
organism
gut metagenome
library construction method
16S Barcoding Kit (SQK-RAB204)
library size
not provided
host taxid
not applicable
environmental medium
DNA/RNA Shield (R1100-50)
geographic location (latitude)
not applicable
scientific_name
gut metagenome
target subfragment
V1-V9
host diet
not applicable
sample storage duration
96h
pcr primers
27F 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R 5'-GGTTACCTTGTTACGACTT-3'
host body product
not applicable
pcr conditions
Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
local environmental context
not applicable
organism count
10.5x10^9
project name
Ultra-deep, hybrid metagenomic sequencing of canine gut microbiome
sequencing method
Oxford Nanopore, SQK-RAB204
host age
not applicable
library screening strategy
ligation based
multiplex identifiers
AAGCGTTGAAACCTTTGTCCTCTC