GUTMCM_minion_v1_9_20240606_macherey_nagel_MN2_bc10
gut metagenome
ENA first public
2024-08-06
INSDC center name
UNIVERSITY OF SZEGED
INSDC status
public
Submitter Id
GUTMCM_minion_v1_9_20240606_macherey_nagel_MN2_bc10
adapters
16SFw 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'; 16S Rev 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'
broad-scale environmental context
not applicable
chimera check software
minitax
collection date
not provided
environmental medium
DNA/RNA Shield (R1100-50)
geographic location (country and/or sea)
not applicable
geographic location (latitude)
not applicable
geographic location (longitude)
not applicable
host age
not applicable
host body product
not applicable
host common name
not applicable
host diet
not applicable
host family relationship
not applicable
host scientific name
not applicable
host sex
not applicable
host subject id
not applicable
host subspecific genetic lineage
not applicable
host taxid
not applicable
library reads sequenced
not provided
library screening strategy
ligation based
library size
not provided
local environmental context
not applicable
multiplex identifiers
AAGCGTTGAAACCTTTGTCCTCTC
nucleic acid amplification
LongAmp Taq 2X Master Mix
nucleic acid extraction
NucleoSpin DNA Stool Mini kit, Macherey Nagel
organism count
10.5x10^9
pcr conditions
Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
pcr primers
27F 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R 5'-GGTTACCTTGTTACGACTT-3'
project name
Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes
sample name
GUTMCM_minion_v1_9_20240606_macherey_nagel_MN2_bc10
sample storage duration
96h
sample storage temperature
-80
scientific_name
gut metagenome
sequence quality check
LongQC
sequencing method
Oxford Nanopore, SQK-16S024
target gene
16S rRNA
target subfragment
V1-V9
temperature
20