MU16_V02_TS
respiratory tract metagenome
ENA first public
2024-08-06
FEV1
NA
INSDC center name
IMU
INSDC status
public
LCI
7.5
Submitter Id
MU16_V02_TS
broad-scale environmental context
Host-associated
broad-scale environmental context
human-associated
chimera check software
zAMP
collection date
2017-02-14
cpneumoniae at visit
No
environmental medium
respiratory system
environmental medium
Throat swab
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.52522
geographic location (longitude)
6.64245
host age
3.8
host sex
male
host subject id
MU16
investigation type
amplicon sequencing
library construction method
paired-end
library screening strategy
PCR
library yield
196
local environmental context
Human
local environmental context
Throat
lung/pulmonary disorder
Cystic fibrosis
mpneumoniae at visit
No
nucleic acid amplification
16S Metagenomic Sequencing Library Preparation (Part. #15044223 Rev. B), Illumina (San Diego, United States)
nucleic acid extraction
MagNA Pure 96 Roche (Basel, Switzerland)
pcr conditions
initial denaturation:95degC_5min; denaturation:95degC_0.5min; annealing:55degC_0.5min; elongation:72degC_0.5min; final elongation:72degC_5min; total cycles:25
pcr primers
FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC
project name
MUCOVIB
project title
Unexpected associations between respiratory viruses and bacteria with Pulmonary Function Testing in children suffering from Cystic Fibrosis (MUCOVIB study)
sample name
MU16_V02_TS
scientific_name
respiratory tract metagenome
sequencing method
Illumina MiSeq
target gene
16S rRNA
target subfragment
V3V4
visit status
Regular visit