BPP8
human gut metagenome
host
Homo sapiens
isolation_source
human feces
collection_date
2022
geo_loc_name
USA: Kannapolis, North Carolina
lat_lon
35.4874 N 80.6217 W
samp_collect_device
A stool collection kit with instructions was given to each participant with instructions to collect the day before visit 3. One Day Prior to Visit 3: Subjects consumed the same dinner that they ate the evening prior to Visit #1, and collected a small swab sample of stool in a container provided by the lab. Sample was stored at room temperature, and returned to the lab within 24 hours. Samples were stored at -20C until processing.
samp_mat_process
DNA was extracted from sixteen human fecal samples and two control samples (water and RNA-later) using the Zymo Quick-DNA™ Fecal/Soil Microbe MicroPrep Kit, according to kit instructions. The protocol was performed in a BSL2 hood, and all biosafety work was approved by the University of Maine Institutional Biosafety Committee (protocol SI-092220). DNA extract concentration was determined using a NanoDrop® ND-1000 UV-Vis Spectrophotometer (Thermo Scientific).
samp_size
5 g
DNA_extract_ng/ul
21
index
CACACAGTAT
index2
TTGAGCCTAA
SFN_nitrile_trtA
14.63
SFN_nitrile_trtB
5.73
First_treatment
B
Percent_GLR_conversion_trtB
12.00361449
Percent_GLR_conversion_trtA
66.69884981
E.coliVL8_Bglucosidase
2.42E+31
E.coli_Bglucosidase3
11026.91935
En.casseliflavus_Bglucosidase4
554.1150744
En.casseliflavus_Bglucosidase44
0
L.agilisR16_msr3
0
Total_others
11581.03442
Btheta2156
17335.82813
Btheta2157
413.1784566
Btheta2158
15441.56771
Btheta2159
5053.737956
Btheta2160
20.34664536
Btheta_operon
38264.6589
BioSampleModel
Metagenome or environmental