toti_minion_WGS_20230802_zymo_Z3
Canis lupus familiaris
chimera check software
minitax
broad-scale environmental context
Csongrad county
host scientific name
Canis lupus familiaris
sequence quality check
LongQC
collection date
2021-12-01
target gene
whole genome
host family relationship
Harsanyi-Tekergo Bator
geographic location (longitude)
20.14824
host subject id
not applicable
host subspecific genetic lineage
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host common name
Hungarian herding terrier: Pumi
host sex
male
temperature
20
nucleic acid extraction
Quick-DNA HMW MagBead Kit, Zymo
nucleic acid amplification
LongAmp Taq 2X Master Mix
geographic location (country and/or sea)
Hungary
library reads sequenced
not provided
sample storage temperature
-80
adapters
Top strand: 5'-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3' / Bottom strand: 5'-ACGTAACTGAACGAAGTACAGG-3'
organism
Canis lupus familiaris
library construction method
Native Barcoding Kit 24 V14 (SQK-NBD114.24)
library size
not provided
host taxid
9615
environmental medium
no
geographic location (latitude)
46.253
scientific_name
Canis lupus familiaris
common name
dog
target subfragment
not applicable
host diet
omnivore
sample storage duration
n.a
pcr primers
not applicable
host body product
stool
pcr conditions
no pcr
local environmental context
Szeged
organism count
not collected
project name
Ultra-deep, hybrid metagenomic sequencing of canine gut microbiome
sequencing method
Oxford Nanopore, SQK-NBD114.24
host age
13 years
library screening strategy
ligation based
multiplex identifiers
AAGGTTACACAAACCCTGGACAAG