Rotterdam wastewater sample sequencing
wastewater metagenome
ENA-FIRST-PUBLIC
2023-03-01
ENA-LAST-UPDATE
2023-03-01
External Id
SAMEA11458565
INSDC center alias
Rotterdam wastewater monitoring team
INSDC center name
Rotterdam wastewater monitoring team
INSDC first public
2023-03-01T08:18:44Z
INSDC last update
2023-03-01T08:18:44Z
INSDC status
public
Submitter Id
iRun102_BC10
broker name
Submission account for department of Viroscience's Research Teams, ErasmusMC
collection date
2021-03-05
collector name
Rotterdam wastewater monitoring team
geographic location (country and/or sea)
Netherlands
geographic location (region and locality)
Rotterdam, Bergschenhoek
investigation type
metagenome
name of the sampling site
wasterwater treatment plant at Bergschenhoek
nucleic acid amplification
RT-PCR
nucleic acid extraction
RNA was isolated with the Biomerieux Nuclisens kit (Biomerieux, Amersfoort, the Netherlands) in combination with the semi-automated KingFisher mL (Thermo Scientific, Bleiswijk, The Netherlands) purification system to extract RNA from Centricon concentrates as previously described (Stinear et al. 1996, 10.1128/aem.62.9.3385-3390.1996; Heijnen et al. 2011, 10.2166/wh.2011.019).
sample name
iRun102_BC10
scientific_name
wastewater metagenome
sewage type
municipal (and industrial) wastewater treatment plant
surveillance target
2697049