BPP10
human gut metagenome
host
Homo sapiens
isolation_source
human feces
collection_date
2022
geo_loc_name
USA: Kannapolis, North Carolina
lat_lon
35.4874 N 80.6217 W
samp_collect_device
A stool collection kit with instructions was given to each participant with instructions to collect the day before visit 3. One Day Prior to Visit 3: Subjects consumed the same dinner that they ate the evening prior to Visit #1, and collected a small swab sample of stool in a container provided by the lab. Sample was stored at room temperature, and returned to the lab within 24 hours. Samples were stored at -20C until processing.
samp_mat_process
DNA was extracted from sixteen human fecal samples and two control samples (water and RNA-later) using the Zymo Quick-DNA™ Fecal/Soil Microbe MicroPrep Kit, according to kit instructions. The protocol was performed in a BSL2 hood, and all biosafety work was approved by the University of Maine Institutional Biosafety Committee (protocol SI-092220). DNA extract concentration was determined using a NanoDrop® ND-1000 UV-Vis Spectrophotometer (Thermo Scientific).
samp_size
5 g
DNA_extract_ng/ul
27.6
index
TACCGCCTCG
index2
CCTCGCAACC
SFN_nitrile_trtA
19.13
SFN_nitrile_trtB
22.33
First_treatment
A
Percent_GLR_conversion_trtB
2.182845534
Percent_GLR_conversion_trtA
5.282765866
E.coliVL8_Bglucosidase
1.13E+20
E.coli_Bglucosidase3
3471.94515
En.casseliflavus_Bglucosidase4
16.81227643
En.casseliflavus_Bglucosidase44
0
L.agilisR16_msr3
0
Total_others
3488.757426
Btheta2156
5610.799154
Btheta2157
121.4066111
Btheta2158
14930.38704
Btheta2159
9824.796712
Btheta2160
9.302605947
Btheta_operon
30496.69212
BioSampleModel
Metagenome or environmental