BPP15
human gut metagenome
host
Homo sapiens
isolation_source
human feces
collection_date
2022
geo_loc_name
USA: Kannapolis, North Carolina
lat_lon
35.4874 N 80.6217 W
samp_collect_device
A stool collection kit with instructions was given to each participant with instructions to collect the day before visit 3. One Day Prior to Visit 3: Subjects consumed the same dinner that they ate the evening prior to Visit #1, and collected a small swab sample of stool in a container provided by the lab. Sample was stored at room temperature, and returned to the lab within 24 hours. Samples were stored at -20C until processing.
samp_mat_process
DNA was extracted from sixteen human fecal samples and two control samples (water and RNA-later) using the Zymo Quick-DNA™ Fecal/Soil Microbe MicroPrep Kit, according to kit instructions. The protocol was performed in a BSL2 hood, and all biosafety work was approved by the University of Maine Institutional Biosafety Committee (protocol SI-092220). DNA extract concentration was determined using a NanoDrop® ND-1000 UV-Vis Spectrophotometer (Thermo Scientific).
samp_size
5 g
DNA_extract_ng/ul
17.7
index
GTAGGCGAGC
index2
TAGGAGCGCA
SFN_nitrile_trtA
0
SFN_nitrile_trtB
0.55
First_treatment
A
Percent_GLR_conversion_trtB
8.983193873
Percent_GLR_conversion_trtA
31.9973049
E.coliVL8_Bglucosidase
2.50E+33
E.coli_Bglucosidase3
147849088.1
En.casseliflavus_Bglucosidase4
2.960400169
En.casseliflavus_Bglucosidase44
0
L.agilisR16_msr3
0
Total_others
147849091.1
Btheta2156
431311.9063
Btheta2157
160311.5469
Btheta2158
35.59574509
Btheta2159
259.8263957
Btheta2160
1.834631562
Btheta_operon
591920.71
BioSampleModel
Metagenome or environmental