Cambium and differentiating xylem, lianescent, rep 6
Bignonia magnifica
isolate
A single plant of Bignonia magnifica cultivated at the gardens of the Biosciences Institute of the University of So Paulo, So Paulo (23.56 S, 46.73 W) as described in the "growth protocol" field was used in the experiment.
dev_stage
Lianescent
geo_loc_name
Brazil: Sao Paulo
tissue
cambium and differentiating xylem
collected_by
Andre C. Lima
growth_protocol
All analyses were performed with clones obtained from cuttings of a single plant of Bignonia magnifica cultivated at the gardens of the Biosciences Institute of the University of So Paulo, So Paulo (23.56 S, 46.73 W), Brazil, to avoid intraspecific variation. One-year-old, one-meter-tall plants were transplanted to 60 cm diameter by 50 cm high pots with soil rich in organic matter in April 2016. Plants were grown besides stainless-steel wire meshes (7x7 cm mesh, 2.1 mm diameter wire), that served as physical support for stem growth. Pots were set 1,5 m apart and watered to a maximum field capacity three times a week for the first year and weekly after that. Tissues were collected in April 2018 by peeling the bark and gently scraping the exposed xylem of 30 cm samples collected along the stems. Before tissue harvest, each stem segment had its base cut and stored in 50 % alcohol to determine the xylem phase, self-supporting or lianescent. Segments presenting dubious or transition anatomy, and the segment next to it, were discarded.
isolation_source
Sao Paulo city has a mean annual temperature of 21.5 o C and a mean total annual precipitation close to 1600 mm.
lat_lon
23.56 S 46.73 W
sample_type
Tissue sample
replicate
6
BioSampleModel
Plant