Viola lutea
ecotype
Blankenrode (Westphalia, western Germany)
age
2 month old seedlings
collection_date
2020
geo_loc_name
Germany: Blankenrode, Westphalia
tissue
Callus
collected_by
Klaudia Sychta
growth_protocol
Sterile leaves of these plants wereharvested and placed on MS medium with different combinations ofauxins and cytokinins: 2 mg L1 2,4-D + 2 mg L1 KIN, 2 mg L1 2,4-D+ 2 mg L1 BAP, or only 0.5 mg L1 TDZ. All plant growth regulatorswere augmented with 30 g L1 sucrose (Sigma-Aldrich) and solidifiedwith 8 g L1 agar (Duchefa Biochemie)
BioSampleModel
Plant