Soybean overexpression of the aldehyde dehydrogenase AhALDH2B6 from Arachis hypogaea under 6 Celsius biological replicate 2
Glycine max
isolate
Low temperature treatment of transgenic plants at 6 Celsius
cultivar
P3
ecotype
not collected
age
9 days of culture
dev_stage
germinating seed stage
geo_loc_name
not collected
tissue
radicles
biomaterial_provider
Northeast Agricultural University, Harbin, China
cell_line
not collected
cell_type
not collected
collected_by
not collected
collection_date
not collected
culture_collection
not collected
disease
not collected
disease_stage
not collected
genotype
not collected
growth_protocol
Peanut, transgenic soybean, and WT seeds in the same growth state were selected. The seeds were disinfected with 70% (v/w) ethanol for 1 min and soaked in 5% (w/v) sodium hypochlorite solution for 15 min, after which the seeds were rinsed 5 to 6 times with sterile water for 5 to 6 times. Soak completely sterilized soybean seeds in 25 C sterile water in a dark growth chamber for 12 h, and peanut seeds need to be soaked for 24 h. For germination stage, The three transgenic strains and WT were seeded in vermiculite and then transferred into a chamber at lower temperature of 6 C, and, we performed RNA-seq analysis using line1-1 and WT, The radicles of transgenic and WT plants treated for 9 d in the cold chamber were used as samples for RNA-seq.
height_or_length
not collected
isolation_source
not collected
lat_lon
45.75 N 126 E
phenotype
not collected
population
not collected
sample_type
not collected
sex
not collected
specimen_voucher
not collected
temp
6
treatment
6
biological_replicate
replicate = biological replicate 2
BioSampleModel
Plant