MI.M06549_0045.001.FLD_ill_039_i7---IDT_i5_4.210309_EDM_B_ATAReuk
biofilm metagenome
DNA extracted from the biofilm of 132.6 cm2 of Ulva thalli from site B and EDM raceway. The V4 variable region of the 18S rRNA gene was targeted using the TAReuk454FWD1 (CCAGCASCYGCGGTAATTCC) forward primer and the TAReukREV3 (ACTTTCGTTCTTGATYRA) reverse primer (Stoeck et al., 2010). Amplicons were obtained by PCR with 25 µL of reactional mix prepared with HotStarTaq polymerase (Qiagen, Hilden, Germany), 33 cycles and a melting temperature of 52 °C.
host
Ulva
isolation_source
Ulva spp. cultivation raceway with Mediterranean seawater
collection_date
2021-03-09
geo_loc_name
France: Marine experimental platform from the French National Institute for Ocean Science or Institut Francais de Recherche pour l Exploitation de la Mer IFREMER
lat_lon
43.52 N 3.9 E
samp_collect_device
Ulva spp. biofilm
samp_mat_process
DNA was extracted using a modified version of the Macherey Nagel NucleoSpin® plant II kit as in Thomas et al 2020 https://doi.org/10.1111/1758-2229.12806 and Brunet al 2021 https://doi.org/10.1111/1462-2920.15389
samp_size
132.6 cm2 of Ulva spp. Thallus
source_material_id
2021-03-09_ulva_biofilm_B_18S
Targeted gene
18S rRNA gene
Date
2021-03-09
Sampling Day
D5
Compartment
biofilm
Condition
SW
Nitrites (µM)
0.07
Nitrates (µM)
1.33
Ammonium (µM)
0.19
Phosphates (µM)
0.03
Silicates (µM)
2.26
Chlorophyll a (µg.L-1)
0.809
Temperature (°C)
10
pH
7.6
BioSampleModel
Metagenome or environmental