M_D0
Mus musculus
Protocols: Salivary gland tissue was excised, enzymatically and mechanically digested and Epcam+ cells (epithelial cells), CD31+ cells (endothelial cells) and CD45+/CD11b+/F480+ cells (macrophages) were isolated using fluorescence-activated cell sorting (FACS) and mixed in the ratio 45%, 45%, 10%, respectively. Isolated cells were loaded into the Chromium Single Cell controller (10x Genomics). Nucleic acid extraction was performed following the manufacturer's instructions (10x Genomics, as part of the Chromium Single Cell 3' v3.1). The four libraries (one for each sample) were pooled for sequencing, taking into account the expected differences in cell numbers between each library.
INSDC center name
The University of Edinburgh
Submitter Id
E-MTAB-13374:M_D0
age
10
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
1340832 and 1340834
isolate
not applicable
organism part
submandibular gland
sample name
E-MTAB-13374:M_D0
scientific_name
Mus musculus
sex
male
strain
C57BL/6J