Stat2KO3
Mus musculus
Protocols: Mice were sacrificed and its colon extracted post-mortem RNA was isolated using the PeqGold Total RNA Kit following manufacturers' instructions (Peqlab/VWR). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.
INSDC center name
Department of Medicine 1, Friedrich Alexander University, Erlangen-Nuremberg, Germany
Submitter Id
E-MTAB-12655:Stat2KO3
age
10
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
disease
experimental colitis
genotype
Stat2 KO
isolate
not applicable
organism part
colon
sample name
E-MTAB-12655:Stat2KO3
sampling site
distal colon
scientific_name
Mus musculus
sex
male
strain
C57BL/6