AA-004-0003-AA
Homo sapiens
Protocols: ScRNA-seq analyses were performed on frozen PBMCs isolated from heparin blood. Samples originated from 8 patients at baseline (4 with SCROT and 4 with SROT). PBMCs were thawed according to 10X Genomics protocol. Cells were counted, diluted at 1,000 cells/mL in PBS+0.04% and 20,000 cells were loaded in the 10x Chromium Controller to generate single-cell gel-beads in emulsion. After reverse transcription, gel-beads in emulsion were disrupted. Barcoded complementary DNA was isolated and amplified by PCR. Following fragmentation, end repair and A-tailing, sample indexes were added during index PCR.
INSDC center name
Service de Medecine Interne, Centre national de reference des cytopenies auto-immunes de l'adulte, Hopital Henri Mondor, Federation Hospitalo-Universitaire TRUE InnovaTive theRapy for immUne disordErs, Assistance Publique Hopitaux de Paris (AP-HP), Universite Paris Est Creteil, Creteil, France.
Submitter Id
E-MTAB-12397:AA-004-0003-AA
age
58
broker name
ArrayExpress
cell_type
nongranular leukocyte
clinical history
sustained complete response off-treatment
common name
human
developmental stage
adult
disease
autoimmune thrombocytopenia
isolate
not applicable
organism part
blood
sample name
E-MTAB-12397:AA-004-0003-AA
scientific_name
Homo sapiens
sex
female