10Ba
marine sediment metagenome
Coastal sediment metagenome taken 15m from a Zostera marina meadow
sample_description
Coastal sediment metagenome taken 15m from a Zostera marina meadow
broad-scale environmental context
temperate marginal sea biome [ENVO:01000856]
collection date
2021-07-28
geographic location (longitude)
12.09713333
sediment environmental package
sediment
run
B
sample material processing
Stored at -80 C
experiment
lib_3
nucleic acid extraction
Samples were thawed and homogenized. A subsample of 500 mg of sediment was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit
geographic location (country and/or sea)
Germany
elevation
-4.4
submitted to insdc
true
organism
marine sediment metagenome
environmental package
sediment
environmental_sample
yes
environmental medium
shallow marine sediment [ENVO:03000034]
geographic location (latitude)
54.18603333
scientific_name
marine sediment metagenome
target subfragment
V3-V4
geographic location (region and locality)
Coastal samples
investigation type
mimarks-survey
total depth of water column
4.4
pcr primers
FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC
local environmental context
microbial community [PCO:1000004]
depth
0-0.01
project name
16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments
sequencing method
Illumina MiSeq
ENA-LAST-UPDATE
2023-11-06