20G_C-1
Rattus norvegicus
Protocols: Primary cell cultures of cortical neurons and cortical astrocytes were generated from tissue collected from embryonic day 17.5 CD1 mice and embryonic day 20.5 Sprague-Dawley rats. Chromatin immunoprecipitation was performed with the SimpleChIP® Plus Enzymatic ChIP Kit (Magnetic Beads) (Cell Signalling Technology, #9005) according to the manufacturer's protocol. Library preparation for the samples were performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs Inc, #E7645S) according to the manufacturer's protocol.
strain
Sprague-Dawley
antibody
H3K27ac
organism
Rattus norvegicus
organism
Rattus norvegicus
collection date
not collected
sex
not applicable
scientific_name
Rattus norvegicus
common name
Norway rat
organism part
brain
developmental stage
embryo stage
genotype
wild type genotype
isolate
not applicable
growth condition
coculture of rat neurons and mouse astrocytes
age
E20.5
geographic location (country and/or sea)
not collected