JS010cDNA
Mus musculus
Protocols: Mouse ESCs (mESCs) and gastruloids were maintained and generated as previously described (see Anlas, Baillie-Benson et al. 2021, Programmed Morphogenesis: Methods and Protocols, p131-147). In brief: mESCs were cultured in DMEM+serum/LIF, including passages every 2-3 days using TrypLE. For Gastruloid generation, mESCs were detached using TrypLE, cell counting was performed and the desired number of cells (usually ~300/well) were plated in 40ul N2B27 into all wells of a round bottom, low adhesion 96-well plate. After incubating for 48h, add 150ul N2B27+3uM CHIR99 (Wnt agonist). NIH3T3 cells were cultured with DMEM, supplemented with 10% Fetal bovine serum, L-glutamine, and Penicillin/Streptomycin. Sample labelling was done with 3' CellPlex Kit (10X Genomics) according to the manufacturer's protocol. Encapusulation and library preparation was perfomed with Chromium Next GEM Single Cell 3ʹ Kit v3.1 by the Chromium Controller (10X Genomics).
ENA-FIRST-PUBLIC
2024-01-09T08:00:07Z
ENA-LAST-UPDATE
2024-01-09T08:00:07Z
External Id
SAMEA114023860
INSDC center name
EMBL
INSDC first public
2024-01-09T08:00:07Z
INSDC last update
2024-01-09T08:00:07Z
INSDC status
public
Submitter Id
E-MTAB-13181:JS010cDNA
broker name
ArrayExpress
cell_type
embryonic stem cells, NIH3T3 cells
collection date
not collected
common name
house mouse
developmental stage
embryo
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-13181:JS010cDNA
sample pool
JS010
scientific_name
Mus musculus