Zea mays B73, seedling primary and seminal root tips (0-1mm), DNA copy number, G1 phase
Zea mays
G1 phase nuclei collected by fluorescence activated nuclei sorting (FANS) based on their 2C DNA content and lack of EdU incorporation.
isolate
not applicable
cultivar
B73
ecotype
not applicable
age
3-days after germination
dev_stage
seedling primary and seminal roots
geo_loc_name
USA:Raleigh NC
tissue
Terminal 1 mm of primary and seminal roots
biomaterial_provider
Linda Hanley-Bowdoin, 851 Main Campus Drive Partners III, rm 362 Raleigh, NC 27606
cell_type
G1 phase
collected_by
Leigh Mickelson-Young, Emily Wear, Emily Wheeler
collection_date
2022-06
growth_protocol
B73 seeds were surface sterilized in 1% NaClO; 0.05% Tween-20, imbibed overnight with stirring and aeration, and surface sterilized again but this time in 0.5% NaClO; 0.05% Tween-20. Seeds were germinated in 6.5x10 inch glass dishes with a clear sealing lid (Rubbermaid Brilliance) with 6 single layers of paper towels dampened with autoclaved dH2O (75 mL per dish). Seedlings were grown in a growth chamber for 3 days with constant dim LED light at 28 C.
sample_type
tissue sample
temp
28 C
treatment
25 uM EdU pulse for 20 min
BioSampleModel
Plant