pt5_inital
Homo sapiens
Patients with recurrent GBM were treated with ICI of pembrolizumab or nivolumab with the addition of bevacizumab. DNA from archival FFPE or OCT tissues was extracted using Maxwell RSC instruments from Promega. FFPE tissues were extracted using Maxwell RSC FFPE Plus DNA kit (AS1720) and protocol. OCT tissues were thawed and extracted using 30uL of Proteinase K, 4 uL of 1-thioglycerol (Promega Part A208B), 266 uL of Sterile water (Nanopure), incubated for 2-3 hours in capped tube at 56oC, then added to Maxwell RSC Blood DNA Cartridge from the AS1400 kit. Add 300 uL of the Lysis Buffer (MC501) provided with the kit to each blood DNA cartridge, well #1, then follow manufactural protocol for Blood DNA extraction. Molecular sequencing was performed on the Ion Torrent platform with Oncomine Comprehensive Assay v3M panel.
isolate
not applicable
age
53
biomaterial_provider
Adilia Hormigo, MD, PhD, Albert Einstein College of Medicine1300 Morris Park Avenue, Rm 702 Golding, Bronx, NY 1046
sex
male
tissue
tumor
disease
Glioblastoma
disease_stage
inital
ethnicity
Other
treatment
Pembrolizumab
Embedding
OCT
Cycles of Concurrent Bevacizumab
16
individual
pt5
BioSampleModel
Human