MI.M06549_0045.001.FLD_ill_096_i7---IDT_i5_11.NEGATIF_ATAReuk
metagenome
DNA extraction without matrix to identify potential contamination from the reagents. The V4 variable region of the 18S rRNA gene was targeted using the TAReuk454FWD1 (CCAGCASCYGCGGTAATTCC) forward primer and the TAReukREV3 (ACTTTCGTTCTTGATYRA) reverse primer (Stoeck et al., 2010). Amplicons were obtained by PCR with 25 µL of reactional mix prepared with HotStarTaq polymerase (Qiagen, Hilden, Germany), 33 cycles and a melting temperature of 52 °C.
host
not applicable
isolation_source
not applicable
collection_date
not applicable
geo_loc_name
not applicable
lat_lon
not applicable
samp_collect_device
not applicable
samp_mat_process
DNA was extracted using a modified version of the Macherey Nagel NucleoSpin® plant II kit as in Thomas et al 2020 https://doi.org/10.1111/1758-2229.12806 and Brunet al 2021 https://doi.org/10.1111/1462-2920.15389
samp_size
not applicable
source_material_id
no Ulva sp. biofilm_18S
Targeted gene
18S rRNA gene
Date
NA
Sampling Day
NA
Compartment
NA
Condition
NA
Nitrites (µM)
NA
Nitrates (µM)
NA
Ammonium (µM)
NA
Phosphates (µM)
NA
Silicates (µM)
NA
Chlorophyll a (µg.L-1)
NA
Temperature (°C)
NA
pH
NA
BioSampleModel
Metagenome or environmental