RNA extracted from Saccharina latissima strain F2.27.2-54.
Saccharina latissima
Culture conditions
F2 sporophytes derived from a bulk gametophyte cross between two strains of Saccharina latissima collected at Perharidy (near Roscoff, north Finistere, Brittany, France, January 2016, strain SLPER139) and Saint Guenole (south Finistere, Brittany, France, October 2015, strain SLSTG055) were cultivated on an open water rope system in Morlaix Bay in 2017.The F1 hybrid was strain F1.27.2. Under laboratory conditions, three small tissue disks cut from the blade of an F2 sporophyte individual were acclimatised for 4 days at 13C and then heat stressed at 21C for 7 days followed by an incubation at 13C for 16 days to allow recovery before harvesting (recovery sample). Fv/Fm measurements were carried out after heat stress and after the recovery period.
DNA or RNA extracted
RNA
DNA or RNA quality (ratio 260/280)
5
ENA first public
2024-05-15
Extraction date
09/11/2023
Extraction method
Macherey-Nagel RNAplus plus 1 wash RW2
Harvest date
19/10/2017 + 17/11/2022
INSDC center name
Genoscope CEA
INSDC status
public
Submitter Id
54-21-1
additional_details
na
collection date
missing: data agreement established pre-2023
generation
sporophyte
geographic location (country and/or sea)
France
geographic location (latitude)
missing: data agreement established pre-2023
geographic location (longitude)
missing: data agreement established pre-2023
identification by
Zofia Nehr
identification method
Morphology
ploidy
diploid
ratio 260/230
2,19
sample alias
54-21-1
sample description
RNA extracted from Saccharina latissima strain F2.27.2-54 for analysis of differential gene expression..
sample name
54-21-1
sampling ecosystem
coastal
sampling sea
Atlantic Ocean
sampling site
Genetic cross of Perharidy and Saint Guenole strains, France
sampling water type
marine
scientific_name
Saccharina latissima
sequencing centre sample code
CIT_FC
sex
na
sex determination method
na
strain
F2.27.2-54
tissue type
blade disks