AD1965_Treated
Homo sapiens
Protocols: Each sample was collected by scrapping the cells from a 10cm cell culture dish. Pellet was stored at -20 degrees. T47D and MDA-MB-231 cells were cultured in RPMI and DMEM medium respectively. The media was supplemented with 10% FBS, 2.5 mg/ml Amphotericin-B (Abbott) and 1.25 μl/ml Gentamycin (Abbott). The cells were cultured at 37 °C in a 5% CO2 incubator. Cells were serum starved followed by treatment with 10nM of progesterone for 6 hours. Equal volume of ethanol was used as vehicle control RNA was extracted using the TRIzol solubilization and extraction protocol, as recommended by the manufacturer. Transcriptome sequencing libraries were constructed by using the TruSeq RNA library protocol (Illumina). Briefly, ribosomal RNA was depleted and library preparation was done as per the manufacturer's instructions (TruSeq RNA Sample Preparation Kit, Illumina).
ENA first public
2024-07-08
External Id
SAMEA110292090
INSDC center name
Advanced Centre for Treatment Research and Education in Cancer (ACTREC)
INSDC last update
2022-07-08T14:44:18Z
INSDC status
public
Submitter Id
E-MTAB-11925:AD1965_Treated
broker name
ArrayExpress
cell_line
T47D
common name
human
developmental stage
adult
disease
breast cancer
genotype
wild type genotype
isolate
not applicable
organism part
breast
sample name
E-MTAB-11925:AD1965_Treated
scientific_name
Homo sapiens