MI.M06549_0045.001.FLD_ill_024_i7---IDT_i5_2.NEGATIF_PCR_2TAReuk
metagenome
molecular grade water used as negative control for the PCR2 for library preparation. The V4 variable region of the 18S rRNA gene was targeted using the TAReuk454FWD1 (CCAGCASCYGCGGTAATTCC) forward primer and the TAReukREV3 (ACTTTCGTTCTTGATYRA) reverse primer (Stoeck et al., 2010). Amplicons were obtained by PCR with 25 µL of reactional mix prepared with HotStarTaq polymerase (Qiagen, Hilden, Germany), 33 cycles and a melting temperature of 52 °C.
host
not applicable
isolation_source
not applicable
collection_date
not applicable
geo_loc_name
not applicable
lat_lon
not applicable
samp_collect_device
not applicable
samp_mat_process
not applicable
samp_size
non communicated by the subcontractor Genome Quebec
source_material_id
molecular grade water 2_18S
Targeted gene
18S rRNA gene
Date
NA
Sampling Day
NA
Compartment
NA
Condition
NA
Nitrites (µM)
NA
Nitrates (µM)
NA
Ammonium (µM)
NA
Phosphates (µM)
NA
Silicates (µM)
NA
Chlorophyll a (µg.L-1)
NA
Temperature (°C)
NA
pH
NA
BioSampleModel
Metagenome or environmental