27Cc
marine sediment metagenome
Coastal sediment metagenome taken 100m from a Potamegaton meadow
ENA first public
2024-05-14
External Id
SAMEA114579306
INSDC center name
IOW Leibniz-Institut fur Ostseeforschung Warnemuende
INSDC last update
2023-11-06T10:23:56Z
INSDC status
public
Submitter Id
131069:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67
broad-scale environmental context
temperate marginal sea biome [ENVO:01000856]
broker name
GFBIO
collection date
2021-07-31
depth
0-0.01
elevation
-1.7
environmental medium
shallow marine sediment [ENVO:03000034]
environmental package
sediment
environmental_sample
yes
experiment
lib_3
geographic location (country and/or sea)
Germany
geographic location (latitude)
54.14366667
geographic location (longitude)
13.726
geographic location (region and locality)
Coastal samples
investigation type
mimarks-survey
local environmental context
microbial community [PCO:1000004]
nucleic acid extraction
Samples were thawed and homogenized. A subsample of 500 mg of sediment was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit
pcr primers
FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC
project name
16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments
run
B
sample material processing
Stored at -80 C
sample name
131069:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67
sample_description
Coastal sediment metagenome taken 100m from a Potamegaton meadow
scientific_name
marine sediment metagenome
sediment environmental package
sediment
sequencing method
Illumina MiSeq
submitted to insdc
true
target subfragment
V3-V4
total depth of water column
1.7