siTfeb_rep1
Mus musculus
Protocols: Dissection of VZs from neonatal mouse brains was performed as described 1. Briefly, the midbrain, the olfactory bulb, meninges and the hippocampus were removed in sequence to attain the telencephalon. Then VZ-containing tissues were separated from the telencephalon. Tissues from 4 mice were pooled, transferred to a 1.5ml Eppendorf tube, and digested with freshly prepared 10U/ml Papain solution (Worthington, catalog no. LS003126) at 37Â°C for 30 minutes. Then the Papain solution was aspirated, and 1ml culture medium (20% FBS) was added, followed by 1 minute of incubation to terminate the digestion. The supernatant was removed, and another 1ml culture medium (20% FBS) was added. The digested tissues were gently pipetted up and down by 20 times with a P1000 tip to dissociate the cells mechanically. Centrifuge at 350rcf for 5 minutes and discard the supernatant.  DNA was purified first by Qiagen minElute PCR purification kit according to the manufacturer's instruction, then by AmupureXP beads with 0.5X upper cutoff and a 1.2X lower cutoff according to manufacturer's instruction. Libraries were constructed in the Illumina Nextera DNA Library preparation kit.
strain
C57BL/6Jax
organism
Mus musculus
organism
Mus musculus
collection date
not collected
stimulus
siTfeb
scientific_name
Mus musculus
common name
house mouse
organism part
ventricular zone
developmental stage
EPC
genotype
wild type genotype
isolate
not applicable
age
0
geographic location (country and/or sea)
not collected