SV-FLB_6_KCNH2_Acc_WGS
Homo sapiens
Protocols: Approximately 1x10^6 hiPSCs (cell line described here: https://hpscreg.eu/cell-line/LUMCi028-A) were rinsed once with PBS without Mg2+ and Ca2+ (PBS-/-) and dissociated to single cells with 1x TrypLE Select (ThermoFisher). The dissociation reagent was neutralized with culture medium (DMEM/F12, BSA 0.5%) and the pellet of cells was resuspended in PBS-/- and kept at -20°C until the genomic DNA extraction protocol was performed. Genomic DNA was extracted from hiPSCs using the High Pure PCR Template Preparation Kit (Roche) and treated with RNAse A (10 mg/ml, Invitrogen) according to the manufacturers' instructions. The nucleic acid library was constructed using the NEBNext® Ultra II FS DNA and Ligation kits to fragment, A-tail and ligate sequencing adapters to the genomic DNA. The size of the resulting product was approximately 500-700 bp.
ENA-FIRST-PUBLIC
2022-09-22
ENA-LAST-UPDATE
2022-09-22
External Id
SAMEA110424416
INSDC center alias
LUMC
INSDC center name
Leiden University Medical Center
INSDC first public
2022-09-22T00:41:14Z
INSDC last update
2022-09-22T00:41:14Z
INSDC status
public
Submitter Id
E-MTAB-11971:SV-FLB_6_KCNH2_Acc_WGS
broker name
ArrayExpress
cell_line
LUMCi028-A
cell_type
induced pluripotent stem cell
common name
human
developmental stage
adult
disease
normal
genetic modification
NC_000007.14:g.150936293-150986864del
genotype
genetically modified genotype
organism part
skin
progenitor cell type
fibroblast
sample name
E-MTAB-11971:SV-FLB_6_KCNH2_Acc_WGS