Zea mays B73, seedling primary and seminal root tips (0-1mm), DNA copy number, EdU labeled S phase
Zea mays
S phase nuclei collected by fluorescence activated nuclei sorting (FANS) based on their DNA content and EdU incorporation.
isolate
not applicable
cultivar
B73
ecotype
not applicable
age
3-days after germination
dev_stage
seedling primary and seminal roots
geo_loc_name
USA:Raleigh NC
tissue
Terminal 1 mm of primary and seminal roots
biomaterial_provider
Linda Hanley-Bowdoin, 851 Main Campus Drive Partners III, rm 362 Raleigh, NC 27606
cell_type
EdU S-phase
collected_by
Leigh Mickelson-Young, Emily Wear, Emily Wheeler
collection_date
2022-06
growth_protocol
B73 seeds were surface sterilized in 1% NaClO; 0.05% Tween-20, imbibed overnight with stirring and aeration, and surface sterilized again but this time in 0.5% NaClO; 0.05% Tween-20. Seeds were germinated in 6.5x10 inch glass dishes with a clear sealing lid (Rubbermaid Brilliance) with 6 single layers of paper towels dampened with autoclaved dH2O (75 mL per dish). Seedlings were grown in a growth chamber for 3 days with constant dim LED light at 28 C.
sample_type
tissue sample
temp
28 C
treatment
25 uM EdU pulse for 20 min
BioSampleModel
Plant