P14601_1008_S4
Larinioides sclopetarius
Protocols: L. sclopetarius adult female spiders were collected in the wild in a small habitat in Uppsala, Sweden. Taxonomic identity of the spider was verified by the Museum of Natural History, Stockholm, Sweden. The spiders were kept in big containers that allowed them to spin webs. They were fed with meal worms or Drosophila flies weekly and watered daily. In total 20 spiders were used for single cell sequencing.  Method A: The major ampullate glands were taken out in ringer solution, pH 7.4. The duct was removed. The glands were washed with PBS, pH 7.4 and then incubated into pre-warmed trypsin-EDTA (Gibco, 0.5%) at 37°C for 1 min in a low-binding micro-centrifuge tube. The glands were triturated with a pipette briefly and centrifuged at 300 g for 3 min at 4°C. The pellets were resuspended in 300 μL DMEM (Gibco) containing 1% BSA (Sigma). The DMEM and the tubes were briefly bubbled with carbogen prior to resuspension. The suspension was strained through a 40 μm cell strainer and transferred to low-binding micro-centrifuge tubes. The library preparation was done using 10X 3' GE kit on the 10X Chromium Single Cell 3' Platform following manufacturer's protocol (Dual Index 10X_3'_V3.1).
strain
not applicable
replicate
8
individual
not applicable
organism
Larinioides sclopetarius
organism
Larinioides sclopetarius
collection date
not collected
sex
female
scientific_name
Larinioides sclopetarius
organism part
Major ampullate gland
silked status
Non-silked
developmental stage
adult
genotype
wild type genotype
isolate
not applicable
collection site
caught in the wild
collection latitude
59.78233273
collection longitude
17.62738681
cell_type
epithelial cell
geographic location
Sweden
geographic location (country and/or sea)
not collected