EN1
biofilm metagenome
Seagrass leave biofilm from station BV13-A, replicate c
ENA first public
2024-05-14
External Id
SAMEA114579241
INSDC center name
IOW Leibniz-Institut fur Ostseeforschung Warnemuende
INSDC last update
2023-11-06T10:23:55Z
INSDC status
public
Submitter Id
131004:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67
broker name
GFBIO
collection date
2021-08-11
environment (biome)
temperate marginal sea biome [ENVO:01000856]
environment (feature)
sea grass bed [ENVO:01000059]
environment (material)
plant matter [ENVO:01001121]
environmental package
host-associated
environmental_sample
Yes
experiment
lib_3
geographic location (country and/or sea)
Estonia
geographic location (depth)
2.1
geographic location (latitude)
59.61413
geographic location (longitude)
25.011633
geographic location (region and locality)
Coastal samples
host-associated environmental package
host-associated
investigation type
mimarks-specimen
nucleic acid extraction
Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit
pcr primers
FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC
project name
16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments
run
B
sample material processing
Stored at -80 C
sample name
131004:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67
sample_description
Seagrass leave biofilm from station BV13-A, replicate c
scientific_name
biofilm metagenome
sequencing method
Illumina MiSeq
submitted to insdc
true
target subfragment
V3-V4
total depth of water column
2.1