EN92
biofilm metagenome
Seagrass leave biofilm from station BV20-A, replicate c
geographic location (depth)
1.2
environment (material)
plant matter [ENVO:01001121]
sample_description
Seagrass leave biofilm from station BV20-A, replicate c
collection date
2021-08-28
geographic location (longitude)
16.3584
run
B
sample material processing
Stored at -80 C
host-associated environmental package
host-associated
experiment
lib_3
nucleic acid extraction
Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit
environment (biome)
temperate marginal sea biome [ENVO:01000856]
environment (feature)
sea grass bed [ENVO:01000059]
geographic location (country and/or sea)
Sweden
submitted to insdc
true
organism
biofilm metagenome
environmental package
host-associated
environmental_sample
Yes
geographic location (latitude)
56.656863
scientific_name
biofilm metagenome
target subfragment
V3-V4
geographic location (region and locality)
Coastal samples
investigation type
mimarks-specimen
total depth of water column
1.2
pcr primers
FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC
project name
16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments
sequencing method
Illumina MiSeq
ENA-LAST-UPDATE
2023-11-06