Homo sapiens
HT1080-CPSF6-mNGreen cells were infected with VSV-G pseudotyped wild type or N74D capsid mutant HIV-1-NL4-3 at a multiplicity of infection (MOI) of 10 by spinoculation or mock-infected. RNA was extracted from each treatment group at 6 hours post-infection, and RNA samples were used for next-generation sequencing. Libraries were prepared using a NEBNext Ultra II kit with poly(A) selection, and samples were sequenced using a NovaSeq X Plus with 150 bp paired-end reads.
isolate
HT1080-CPSF6-mNGreen cells
age
n/a
biomaterial_provider
Dr. Paul D. Bieniasz
collection_date
n/a
geo_loc_name
n/a
sex
male
tissue
Epithelial cells derived from connective tissue from a patient with Fibrosarcoma
cell_line
HT1080
cell_type
Epithelial
disease
Fibrosarcoma
sample_type
Cell culture
treatment
Infected with VSV-G Pseudotyped HIV-1 NL4-3, Technical Replicate 2
BioSampleModel
Human