Fig7_NM_DIV3_N2
Rattus norvegicus
Protocols: Primary cell cultures of cortical neurons and cortical astrocytes were generated from tissue collected from embryonic day 17.5 CD1 mice and embryonic day 20.5 Sprague-Dawley rats. RNA extraction was carried out using the High Pure RNA Tissue kit (Roche) as per manufacturer's instructions. Barcoded RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer's instructions.
strain
Sprague-Dawley
individual
not applicable
organism
Rattus norvegicus
organism
Rattus norvegicus
collection date
not collected
sex
not applicable
stimulus
none
scientific_name
Rattus norvegicus
common name
Norway rat
organism part
brain
developmental stage
embryo stage
genotype
wild type genotype
isolate
not applicable
growth condition
neuronal monoculture
age
E20.5
geographic location (country and/or sea)
not collected