Canis lupus familiaris
Protocols: Two cloned dogs(NT-2, NT-4) were produced via SCNT using ear fibroblasts from a 5-year-old male German shepherd. For whole genome sequencing, blood and ear fibroblasts were collected from a 6-year-old male German shepherd dog, and blood samples were collected from  4-month-old cloned dogs. Experiments were authorized by the Animal Center for Biomedical Experimentation at the National Institute of Animal Science of the Rural Development Administration (approval number 2015-143 on 21 May 2015). Genomic DNA was extracted from the blood of cloned dogs and original dog with a Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) and from original dog cells with PureLink™ Genomic DNA Mini Kit (Thermo Fisher Scientific, Carlsbad, CA, USA). Individual libraries were generated TruSeq Nano DNA Sample Prep Kit (Illumina, San Diego, CA, USA).