Engystomops pustulosus
The Tungara frog (Engystomops pustulosus) is a species of frog in the family Leptodactylidae. It is a small nocturnal terrestrial frog found in Mexico, Central America, and the northeastern regions of South America. The tungara frog exhibits interesting behavior in male/female interactions. Male vocalizations are critical in female mate choice, and females often prefer males who give complex mating calls at a lower frequency rather than simple calls at a higher frequency (from Wikipedia entry). This sample of a female individual (aEngPut2/MGP-1444.1) is part of a trio assembly (offspring aEngPut4/MGP-1488.2, father aEngPut3/MGP-1452.1), with Hi-C sequence data from the sister offspring aEngPut1/MGP-1488.1 used for scaffolding. The individuals were bred in captivity and procured by Marcos Gridi-Papp to generate a high-quality reference genome for the Vertebrate Genomes Project (VGP). Sequencing and genome assembly were conducted at the Vertebrate Genome Lab (VGL) at the Rockefeller University, led by Erich D. Jarvis, Giulio Formenti, and Jennifer Balacco.