Mus musculus
YFP-Parkin-MEFs were lentivirally transduced with the mouse CRISPR-Cas9 knockout (GeCKOv2) library at 0.3 MOI, treated or not with two concentrations of valinomycin (300 nM or 600 nM) for three rounds of treatment and enriched by clonogenic survival, for pools of cells expressing multiple gRNAs targeting the same gene. Genomic DNA was harvested and prepared for NGS