Beijerinckiaceae bacterium RH AL1
Protocols: Biomass was harvested from mid-exponential cultures by means of centrifugation. Beijerinckiaceae bacterium RH AL1 was grown using MM2 medium to mid-exponential phase, using methanol (0.5%, v/v) as carbon and energy source. Cultures were supplemented with either different concentrations of lanthanum (50 nM, 1 µM) or different lanthanide species (La, Nd, lanthanide cocktail [La, Ce, Nd, Dy, Ho, Er, Yb]; 1 µM). Total RNA was extracted with TRI reagent (Applied Biosystems), mRNA was enriched using the RiboMinus bacteria 2.0 transcriptome isolation kit (Invitrogen). Sequencing libraries were prepared with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs).