Homo sapiens
Protocols: Cells were washed with PBS, trypsinized, and kept in -80 until RNA extraction. ATM-KO colonies formed out of single cells were plated on mouse feeder cells in 6-well plates. Cells were treated with 10uM LATS1/2 inhibitor (TRULI, Lats-IN-1) for 24h or left untreated RNA was extracted using NucleoSpin RNA Plus kit mRNA fraction of total RNA was enriched by pulldown of poly(A)-RNA. RNA sequencing libraries were generated using TruSeq RNA Library Prep Kit (Illumina) according to the manufacturer's protocol.