Mus musculus
Protocols: GEMM prostate glands were harvested and snap frozen in RNase-free conditions. Total RNA was extracted from GEMM prostates as described in following details. Briefly, the entire mouse prostates were disrupted with pestle (Axygen PES-15-B-SI Disposable Tissue Grinder Pestle) in 1.5 ml Eppendorf tubes loaded with 350 µl of RLT buffer (RNeasy® Mini Kit, Qiagen, cat. Numb. 74104). RNA was extracted by following RNeasy® Mini Kit instructions. The RNA quality was verified by the spectrophotometric analysis by using 2100 Bioanalyzer System (Agilent, cat. Numb. G2939BA). Only samples with R.I.N. (RNA integrity number) 8-10 were used for Poly-A RNA sequencing analysis. NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.