Homo sapiens
Protocols: Single cell suspensions (scRNA seq) or single nuclei suspensions (Multiome) were obtained according to manufacturer's instructions (10X Genomics). scRNA-seq and Multiome (ATAC + gene expression). Library preparation was performed according to the manufacturer's instructions (single cell 3' v2.1 or v3 protocol, Multiome 10x Genomics). First, reverse transcription was performed in droplet and library construction was performed, according to the 10X Chromium Single Cell 3' Reagent Kit User Guide Rev C. From the same cDNA of the total RNA library, additional libraries were obtained for GBC samples. This was performed in order to increase the reads of the specific barcodes. GBC libraries were prepared a dial-out PCR strategy to amplify the BFP 3'UTR region containing the barcode, as in Dixit A et al. DOI 10.1016/j.cell.2016.11.038.