CB_Phd2KO_A
Mus musculus
Protocols: Tyrosine hydroxylase-restricted Phd2 knockout mice were generated by inter-crossing Phd2f/f and THCre strains as described by Lindeberg et al., 2004 and Mazzone et al., 2009. Carotid bodies or adrenal medullas were sub-dissected and pooled from 5 Phd2 knockout or 5 control (THCre negative or Phd2 wildtype) mice. Tissues were stored in ice-cold RNAProtect (Qiagen) until processing. Tissues stored in RNAProtect were washed with PBS, resuspended in RLT Buffer (Qiagen) supplemented with dithiothreitiol (Merck), and homogenized with Pro200 homogenizer (ProScientific). RNA was extracted from the lysate using RNeasy Plus Micro Kit (Qiagen) according to the manufacturer's instructions and as described previously by Gao et al., 2017. RNA sequencing library was prepared using the NEBNext Single Cell/Low Input RNA Library Prep kit (NEB).
INSDC center name
Max Delbrück Center for Molecular Medicine
Submitter Id
E-MTAB-13163:CB_Phd2KO_A
age
approx. 2
batch
A
broker name
ArrayExpress
collection date
not collected
common name
house mouse
genotype
Phd2 knockout
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
carotid body
sample name
E-MTAB-13163:CB_Phd2KO_A
scientific_name
Mus musculus
strain
C57BL/6