mDC1
Homo sapiens
Protocols: Immediately after thawing, PBMCs were stained with antibody cocktails and pDCs were sorted by FACS according to the CD123 (IL-3R) and BDCA-2 (CD303) expressions. As an internal control, mDCs were isolated from the same PBMC donors and simultaneously sorted by FACS according to the CD11c expression. RNA was extracted from the 1 x106 cells by QIAzol Lysis Reagent (QIAGEN). Concentrations of nucleic acids were measured by nanodrop (all experiments) and/or Qubit HS RNA-kit (only for RNA-seq). RNA quantity and quality were measured by Agilent 2100 Bioanalyzer (Agilent technologies). RNA integration values (RIN) >8 were considered to proceed to the further analysis. RNA-seq libraries were generated using Illumina TruSeq Stranded Total RNA kits. The resultant cDNA libraries were quantified via Qubit and assessed for quality via Bioanalyzer.
ENA-FIRST-PUBLIC
2023-10-24T00:20:36Z
ENA-LAST-UPDATE
2023-10-24T00:20:36Z
External Id
SAMEA114464228
INSDC center alias
Jikei University School of Medicine
INSDC center name
Jikei University School of Medicine
INSDC first public
2023-10-24T00:20:36Z
INSDC last update
2023-10-24T00:20:36Z
INSDC status
public
Submitter Id
E-MTAB-13390:mDC1
broker name
ArrayExpress
cell_type
conventional dendritic cell
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blood
sample name
E-MTAB-13390:mDC1
scientific_name
Homo sapiens