untagged_ctrl_input_3
Schizosaccharomyces pombe
Protocols: Cells were grown in YES and harvested by centrifugation after crosslinking with 1% formaldehyde for 20 min at room temperature. ChIP experiments were performed as described in Wittmann et al., Nat Commun 8, 14861 (2017). HTP-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific). Sequencing libraries were constructed with the TruSeq ChIP Library Preparation Kit (Illumina).
strain
YP144
isolate
not applicable
organism
Schizosaccharomyces pombe
organism
Schizosaccharomyces pombe
collection date
not collected
scientific_name
Schizosaccharomyces pombe
common name
fission yeast
organism part
whole organism
genotype
h+ leu1-32 ura4D18 ade6-M216
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2024-01-20