nmtDbp2_1_input_t9
Schizosaccharomyces pombe
Protocols: Cells were grown in EMMG, then shifted to YES for 9h. S. cerevisiae spike-in cells were added at at 1:5 OD600 ratio immediately before crosslinking with 1% formaldehyde for 20 min at room temperature. ChIP experiments were performed as described in Wittmann et al., Nat Commun 8, 14861 (2017). RNAPII was immunoprecipitated with antibody against Rpb1 (8WG16, Millipore) coupled to 20 µl of protein-G dynabeads (Life Technologies). Libraries were prepared with the NEBNext Fast DNA Library Prep Set for Ion Torrent Kit (NEB).
ENA first public
2024-07-01
External Id
SAMEA115121254
INSDC center name
Institute of Biochemistry Justus-Liebig University Giessen
INSDC last update
2024-01-20T13:28:22Z
INSDC status
public
Submitter Id
E-MTAB-13714:nmtDbp2_1_input_t9
broker name
ArrayExpress
collection date
not collected
common name
fission yeast
genotype
h+ leu1-32 ura4D18 ade6-M216 his3-D1 dbp2::ura4:P.nmt1-dbp2-3myc:kanMX
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-13714:nmtDbp2_1_input_t9
scientific_name
Schizosaccharomyces pombe
strain
YP818