WM983B_3
Homo sapiens
Protocols: Primary human melanoma cell lines were purchased from Rockland and cultured in MCDB153 (ref. n°M7403, Merck/Sigma-Aldrich) and Leibovitz's L-15 medium (ref. n°11415064, Thermo Fisher Scientific) in a 4 to 1 ratio, supplemented with 2% foetal bovine serum, 1.68 mM CaCl2 and 1% penicillin/streptomycin.  RNA was isolated from melanoma cell lines using the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA integrity was evaluated on an Agilent Bioanalyzer 2100 using an Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA).  Sequencing libraries were prepared using “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” combined with “NEB Ultra II polyA mRNA magnetic isolation” for mRNA enrichment (New England Biolabs, Ipswich, MA, USA).
INSDC center name
University of Strasbourg
Submitter Id
E-MTAB-13165:WM983B_3
broker name
ArrayExpress
cell_line
WM983B
collection date
not collected
common name
human
developmental stage
adult
disease
melanoma
disease staging
metastatic
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
skin
sample name
E-MTAB-13165:WM983B_3
scientific_name
Homo sapiens