Potter Cove sediment slurry incubation - birnessite.DIC - day 10 nucleic acid extract
marine sediment metagenome
Slurry incubation experiment with birnessite, DIC (dissolved inorganic carbon), timepoint day 10, incubated at 2 degrees C,  sediment from station 01, core STA01.02, Potter Cove, Antarctica; geographic location, depth, collection date parameters refer to sediment used to prepare the slurry; experiment set up in biological triplicates; collection timepoint and collection date experiment refer to sampling of experiment
sample volume or weight for DNA extraction
2
source material identifiers
slurry prepared in ratio 1:5 of sediment Potter Cove core STA01.02 and sulfate-free artificial sea water
collection timepoint
10
broad-scale environmental context
shallow marine sediment [ENVO:03000034] | polar biom [ENVO:01000339] | glacial retreat [ENVO:01001656] | coast [ENVO:01000687]
collection date
2019-01-22
geographic location (longitude)
-58.640306
collection date experiment
2023-02-17
sample collection device
syringe, needle
sample material processing
slurry  sample was frozen at -80 degrees C until combined DNA/RNA extraction
substrate added experiment
birnessite, DIC
oxygenation status of sample
anaerobic
relationship to oxygen
anaerobe
experimental factor
time series design [EFO:0001779] | different substrate additions
geographic location (country and/or sea)
Antarctica
elevation
-10.8
amount or size of sample collected
0.002
organism
marine sediment metagenome
replicate experiment
B
environmental medium
microbial community [PCO:1000004] | mesocosm [ENVO:01000620]
geographic location (latitude)
-62.226156
scientific_name
marine sediment metagenome
sample collection method
slurry sampling in anoxic manner
geographic location (region and locality)
King George Island/Isla 25 de Mayo, Potter Cove
local environmental context
anaerobic sediment [ENVO:00002045]
depth
0 - 0.25
project name
Bacterial 16S rRNA community in manganese reducing slurry incubation experiment with sediment of Potter Cove