Homo sapiens
Replicate_1
isolate
Cell line
age
Passage 50
biomaterial_provider
Inje University, Innovative Therapeutics Research Institute
collection_date
2022-09-19
geo_loc_name
South Korea: Busan
sex
male
tissue
Lung
cell_line
A549
cell_type
Epithelial cell
dev_stage
Day 3 post-infection
treatment
A549 cells were infected with lentivirus overexpressing pCMV-BTNL9-AM-tag for 3 days before formaldehyde fixative
growth_protocol
RPMI-1640 medium with 10% fetal calf serum
Extraction protocol
To prepare input DNA, infected A549 cells with lentivirus were prepared using Tag-ChIP-IT (Active Motif, #53022) according to the manufacturers protocol to purify initial input DNA. Input DNA with replicates were sent to ChIP-seq service provider (BGI Genomics, Hong Kong) for further processing and analysis. ChIP-DNA was sequenced using the DNBSEQ sequencing technology platforms, and the library preparation and sequencing were performed according to the manufacturers protocol.
BioSampleModel
Human