HCC827_sgNF2_R3
Homo sapiens
Protocols: ~2 million cells per sample were lysed in 350uL of RLT and stored at -80 until subsequent extraction. RNA was extracted using the RNeasy 96 QIAcube HT Kit (Qiagen) with a DNAse digest as per manufactures recommendations. Libraries were prepared using mRNA Stranded library preparation kit (Illumina) and subsequently quantified by Qubit and KAPA library quantification kit, ROX low (Roche). Library sizes were also determined by bioanalyzer.
ENA first public
2024-07-01
INSDC center name
AZ
INSDC status
public
Submitter Id
E-MTAB-13831:HCC827_sgNF2_R3
broker name
ArrayExpress
cell_line
HCC827
cell_type
immortal cell line cell
collection date
not collected
common name
human
developmental stage
adult
disease
non-small cell lung adenocarcinoma
genetic modification
NF2 KO
genotype
EGFR mutant
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lung
replicate
3
sample name
E-MTAB-13831:HCC827_sgNF2_R3
scientific_name
Homo sapiens