Sample 1
Homo sapiens
Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher).
INSDC center name
Cardiff University School of Biosciences European Cancer Stem Cell Research Institute
Submitter Id
E-MTAB-13161:Sample 1
broker name
ArrayExpress
cell_line
hGBM
cell_type
astrocyte
collection date
not collected
common name
human
developmental stage
adult
disease
glioblastoma multiforme
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
line
L0
organism part
brain
sample name
E-MTAB-13161:Sample 1
scientific_name
Homo sapiens