Sample 15
Homo sapiens
Protocols: Human peripheral blood monocytes were isolated from fresh peripheral blood drawn with EDTA anticoagulation. Monocytes were purified by magnetic-activated cell sorting (MACS) with magnetic microbeads conjugated to anti-CD14 antibodies (positive selection) as per manufacturer's instructions (Miltenyi). Monocytes were cultured in RPMI1640 with 10% FCS and 1% penicillin/streptomycin supplementation at 37 °C in 5% CO2 humidified atmosphere on 24-well polystyrene cell culture plates. Peripheral blood monocytes were stimulated with cell-culture grade (\"BioXtra\"), γ-irradiated lipopolysaccharides from Escherichia coli 0111:B4 (Sigma-Aldrich) dissolved in PBS to 1 µg/mL and diluted 1:1000 in cell-culture media to a final concentration of 1 ng/mL for 4 hours in technical duplicates. PBS was used as a vehicle control. 10^6 monocytes were lysed in situ with RLT+ buffer and genomic DNA contamination reduced with column-based genomic DNA removal as per suppliers' instructions (Qiagen). Total RNA was purified with the RNeasy Plus Kit as per manufacturer's instructions. Transcriptome libraries were prepared from 200 ng of total RNA with the TruSeq RNA Sample Preparation Kit v2, Set B (Illumina) as per supplier's instructions.
ENA-FIRST-PUBLIC
2023-07-25T12:00:09Z
ENA-LAST-UPDATE
2023-07-25T12:00:09Z
External Id
SAMEA114192996
INSDC center alias
University of Ulm
INSDC center name
University of Ulm
INSDC first public
2023-07-25T12:00:09Z
INSDC last update
2023-07-25T12:00:09Z
INSDC status
public
Submitter Id
E-MTAB-13200:Sample 15
broker name
ArrayExpress
clinical information
neurologically unaffected
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
individual
HC8
isolate
not applicable
organism part
blood
sample name
E-MTAB-13200:Sample 15
scientific_name
Homo sapiens