WT3
Danio rerio
Protocols: The 48 hpf gata1+ erythrocytes were sorted (FACSAria, BD Bioscience, CA, USA) Two independent groups of RNA from wt and kdm4b-/- erythrocytes were extracted using TRIzol® Reagent (Life Technologies) (each group contains three biological repeats). After adenylation of 3' ends of double strand cDNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase. At last, PCR products were purified and the library quality was assessed on the Agilent Bioanalyzer 2100 system.
strain
Tubingen
disease
normal
individual
mixed pool of 200 individuals
organism
Danio rerio
organism
Danio rerio
collection date
not collected
sex
mixed
scientific_name
Danio rerio
common name
zebrafish
organism part
embryo
developmental stage
embryo stage
genotype
wild type genotype
isolate
not applicable
immunophenotype
gata1 positive
cell_type
erythrocyte
age
48
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2023-06-27