Sample 1
Mus musculus
Protocols: AT3 tumor-bearing mice were euthanized, and blood and lungs were harvested. Neutrophils were then sorted from blood and lung for RNA-seq. Cells were lysed and homogenized in TRIzol Reagent (ThermoFisher), then RNA was isolated using the miRNeasy Mini kit (Qiagen), according to manufacturers' protocols, including the optional DNase digest step. Libraries were constructed using the KAPA mRNA HyperPrep Kit (Roche Sequencing and Life Science), according to the manufacturer's protocol. Briefly, the protocol entails isolation of polyA containing mRNA using oligo-dT magnetic beads, RNA fragmentation, first and second strand cDNA synthesis, ligation of Illumina-specific adapters containing a unique barcode sequence for each library, and PCR amplification. The quality and concentration of the libraries were assessed using the D5000 ScreenTape (Agilent Technologies) and Qubit dsDNA HS Assay, respectively, according to the manufacturers' instructions.
ENA-FIRST-PUBLIC
2022-12-12
ENA-LAST-UPDATE
2022-12-12
External Id
SAMEA112136908
INSDC center alias
The Jackson Laboratory
INSDC center name
the jackson laboratory
INSDC first public
2022-12-12T00:22:04Z
INSDC last update
2022-12-12T00:22:04Z
INSDC status
public
Submitter Id
E-MTAB-12365:Sample 1
age
10
broker name
ArrayExpress
cell_type
neutrophil
common name
house mouse
developmental stage
adult
genotype
breast cancer
genotype
wild type genotype
individual
Individual 1
organism part
blood
sample name
E-MTAB-12365:Sample 1
sex
female
strain
C57BL/6J