5698STDY7839918_CS_GEX
Homo sapiens
Protocols: Protocol carried out by Barker lab to extract an organ specimen from a paediatric donor Libraries were prepared according to the standard protocol of the Chromium 5'V1 VDJ kit. An in-house kit was used for gdTCR enrichment.  V(D)J libraries were prepared according to the manufacturerâ€™s protocol (10X Genomics) using individual Chromium i7 Sample Indices.5,000 PE reads per cell for V(D)J libraries were aimed for sequencing. Libraries for gamma/delta TCR variable regions were amplified as previously described (Mimitou et al. 2019)
disease
normal
individual
BRC2153
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
female
scientific_name
Homo sapiens
common name
human
organism part
heart
developmental stage
Carnegie stage 22
sampling site
cardiac atrium
isolate
not applicable
protocol
10x 5' v1
age
8
geographic location (country and/or sea)
not collected