SIGAC10_CS_3'v2
Homo sapiens
Protocols: Protocol carried out by HDBR to extract specimen from a fetal donor.  Nucleic acid was extracted according to the standard protocol of the Chromium single cell 3' V2 kit. Libraries were prepared according to the standard protocol of the Chromium single cell 3' V2 kit. V(D)J libraries were prepared according to the manufacturer's protocol (10X Genomics) using individual Chromium i7 Sample Indices.5,000 PE reads per cell for V(D)J libraries were aimed for sequencing. Libraries for gamma/delta TCR variable regions were amplified as previously described (Mimitou et al. 2019)
disease
normal
individual
HDBRL13930
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
female
scientific_name
Homo sapiens
common name
human
organism part
lung
developmental stage
20th week post-fertilization human stage
sampling site
whole lung
isolate
not applicable
protocol
10x 3' v2
age
20
geographic location (country and/or sea)
not collected