CGD03 V2
Homo sapiens
Protocols: IFN-γ administration was initiated at the dose of 50 mcg/m2 given subcutaneously on the standard schedule of Monday, Wednesday, and Friday in the evening before bedtime. Then, 10-12 hours after the 1st dose, and the 4th dose of IFN-γ additional blood samples were obtained. Neutrophils were isolated from heparinized peripheral blood by Dextran sedimentation, Ficoll-Hypaque density centrifugation, and hypotonic lysis of red blood cells 36,37. Neutrophils were quantitated manually in a hemocytometer in the presence of 3% paraformaldehyde. RNA from neutrophils was isolated by standard technique. RNA was used to prepare libraries using the Universal Plus mRNA-seq kit from Tecan Genomics (Redwood City, CA).
dosing time
Baseline blood draw
disease
chronic granulomatous disease
individual
CGD03
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
male
scientific_name
Homo sapiens
common name
human
organism part
blood
compound
IFNG
developmental stage
juvenile
isolate
not applicable
dose
50
cell_type
neutrophils
age
17.9 YO
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2023-12-22