WT1_RNAPII_IP_t0
Schizosaccharomyces pombe
Protocols: Cells were grown in EMMG, then shifted to YES for 9h. S. cerevisiae spike-in cells were added at at 1:5 OD600 ratio immediately before crosslinking with 1% formaldehyde for 20 min at room temperature. ChIP experiments were performed as described in Wittmann et al., Nat Commun 8, 14861 (2017). RNAPII was immunoprecipitated with antibody against Rpb1 (8WG16, Millipore) coupled to 20 Âµl of protein-G dynabeads (Life Technologies). Libraries were prepared with the NEBNext Fast DNA Library Prep Set for Ion Torrent Kit (NEB).
strain
YP805
isolate
not applicable
organism
Schizosaccharomyces pombe
organism
Schizosaccharomyces pombe
collection date
not collected
scientific_name
Schizosaccharomyces pombe
common name
fission yeast
organism part
whole organism
genotype
h+ leu1-32 ura4D18 ade6-M216 dbp2::dbp2-3myc:kanMX
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2024-01-20