Homo sapiens
independent clone from HEK293T stably transfected with rtTA, transduced with inducible constructs encoding for rat APOBEC1 and human RBM47, induced with doxycycline. In order to generate inducible APOBEC1 and RBM47 expressing cell lines, we used Tet-inducible APOBEC1 and RBM47 lentiviral expression vectors in HEK293-T cell lines expressing rTTA3. The APOBEC1 expression construct bore a EGFP coding sequence downstream the APOBEC1 one, connected through an IRES element. Cells were transduced sequentially with the lentiviral constructs and sorted after induction of the EGFP to obtain single clones. APOBEC1 expression was induced by doxycycline (2 ug/ml doxycycline for 48 hours [Sigma-Aldrich #D9891]). Two days post-induction  cells were collected and the RNA was extracted.
isolate
clone 87 APOBEC1+RBM47 +doxycycline
age
not applicable
biomaterial_provider
not applicable
collection_date
not applicable
geo_loc_name
not applicable
sex
not applicable
tissue
not applicable
cell_line
HEK293T
BioSampleModel
Human