SQ_2070
Homo sapiens
Protocols: Peripheral blood mononuclear cells were magnetically enriched for CD4+ T-cells with a kit (Miltenyi). T-cells were stained with antibodies to CD4, IL-7R, CD25, CCR6, CXCR3 and CCR5 and purified on a FACS Aria (BD). CD4+IL-7R+CD25-/lo helper T-cells were purified as cTh17- (CCR6+CXCR3-CCR5-), pTh17- (CCR6+CXCR3-CCR5+), CCR5-Th1/17- (CCR6+CXCR3+CCR5-) or CCR5+Th1/17-cells (CCR6+CXCR3+CCR5+). RNA was isolated using mirVana Isolation Kit (ThermoFisher) Library construction was performed with the TruSeq RNA Sample Preparation Kit v2 (Illumina)
ENA-FIRST-PUBLIC
2023-07-26T08:00:09Z
ENA-LAST-UPDATE
2023-07-26T08:00:09Z
External Id
SAMEA114193818
INSDC center alias
INGM-National Institute of Molecular Genetics “Romeo ed Enrica Invernizzi”, Milan, Italy
INSDC center name
INGM-National Institute of Molecular Genetics “Romeo ed Enrica Invernizzi”, Milan, Italy
INSDC first public
2023-07-26T08:00:09Z
INSDC last update
2023-07-26T08:00:09Z
INSDC status
public
Submitter Id
E-MTAB-13178:SQ_2070
broker name
ArrayExpress
cell_type
T cell
collection date
not collected
common name
human
developmental stage
adult
disease
Crohn's disease
genotype
wild type genotype
geographic location (country and/or sea)
not collected
immunophenotype
CCR5+ Th1/17
individual
CD donor 3
isolate
not applicable
organism part
intestine
sample name
E-MTAB-13178:SQ_2070
scientific_name
Homo sapiens