NAA18h_3
Arabidopsis thaliana
Protocols: root sections between 1000 µm and 2000 µm from the root tip were microdissected Col-0 seeds were germinated on medium containing 10 μM NPA and transferred 3 d after germination under continuous light to 10 μM NAA, according to the points of the time course. total RNA was extracted using Trizol (Invitrogen) and the RNeasy Plant mini kit (Qiagen) Using the Illumina TruSeq® Stranded mRNA Sample Prep Kit poly-A containing mRNA molecules were purified from the total RNA input using poly-T oligo-attached magnetic beads. In a reverse transcription reaction using random primers, RNA was converted into first strand cDNA and subsequently converted into double-stranded cDNA in a second strand cDNA synthesis reaction using DNA PolymeraseI and RNAse H. The cDNA fragments were extended with a single 'A' base to the 3' ends of the blunt-ended cDNA fragments after which indexing adapters were ligated introducing single distinct barcodes for each sample. Finally, enrichment PCR was carried out to enrich those DNA fragments that have adapter molecules on both ends and to amplify the amount of DNA in the library.
ENA first public
2024-07-01
External Id
SAMEA113937326
INSDC center name
Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium
INSDC last update
2023-06-23T14:42:16Z
INSDC status
public
Submitter Id
E-MTAB-13112:NAA18h_3
age
3
broker name
ArrayExpress
collection date
not collected
common name
thale cress
developmental stage
seedling development stage
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
root
sample name
E-MTAB-13112:NAA18h_3
sampling site
root 1-2 mm from tip
scientific_name
Arabidopsis thaliana
ecotype
Col-0