Srp2HTP_IgG_IP_2
Schizosaccharomyces pombe
Protocols: Cells were grown in YES and harvested by centrifugation after crosslinking with 1% formaldehyde for 20 min at room temperature. ChIP experiments were performed as described in Wittmann et al., Nat Commun 8, 14861 (2017). HTP-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific). Sequencing libraries were constructed with the TruSeq ChIP Library Preparation Kit (Illumina).
ENA first public
2024-07-01
External Id
SAMEA115121245
INSDC center name
Institute of Biochemistry Justus-Liebig University Giessen
INSDC last update
2024-01-20T13:19:14Z
INSDC status
public
Submitter Id
E-MTAB-13712:Srp2HTP_IgG_IP_2
broker name
ArrayExpress
collection date
not collected
common name
fission yeast
genotype
h+ leu1-32 ura4D1 ade6-M2 srp2::srp2-(his6-TEV-protein A):kanMX6
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-13712:Srp2HTP_IgG_IP_2
scientific_name
Schizosaccharomyces pombe
strain
YPCK172