AN14_Liver_S13
Rattus norvegicus
Protocols: Representative samples of blood, gross lesions, kidney, liver, thymus and uterus were collected from all animals and preserved in 10% neutral buffered formalin or snap frozen in liquid nitrogen and stored in deep freezer (<-75°C). Organs were collected at scheduled necropsy. The total RNA of all 96 samples were extracted using different methods depending on the tissue of origins. Solid tissues (Liver, Kidney and Thymus) were extracted using the miRNeasy Mini Kit (Qiagen, Cat.No. 217004). For this, small pieces of tissue were cut and dissolved in 700 µl of Qiazol using the MiniBeadBeaterPlus for two cycles of 15 seconds and 5 minutes, respectively. The blood was processed with the RNeasy Protect Animal Blood Kit, (Qiagen , Cat. No. 73224). All extracted total RNAs were then assessed for quality and dosed using a bioAnalyser tracted total RNA were first treated by Ribo-depletion using the NEXTFLEX Ribonaut rRNA Depletion. For this, 1ug of total were used for all solid tissue, and 143 ng for the blood (corresponding of the smallest extracted amount for animal 19). Library preparation was then made using the NEXTFLEX Rapid Directional RNA-Seq Kit 2.0, with the corresponding unique dual indexes
ENA first public
2025-01-29
INSDC center name
Maastricht University
INSDC status
public
Submitter Id
E-MTAB-13996:AN14_Liver_S13
age
13
broker name
ArrayExpress
collection date
not collected
common name
Norway rat
genotype
AHR-
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Liver
sample name
E-MTAB-13996:AN14_Liver_S13
scientific_name
Rattus norvegicus
sex
female
strain
Sprague Dawley rats