Rat ID 28 High Isoflavone diet.
rat gut metagenome
collection_date
2022-11
env_broad_scale
gut(UBERON:0001007)
env_local_scale
colon(UBERON:0001155)
env_medium
feces(UBERON:0001988)
geo_loc_name
USA:Arkansas\,Little Rock (GAZ\,00165676)
host
Rattus norvegicus (FBsp:00000020)
lat_lon
not applicable
chem_administration
AIN-93G diet, casein as the protein source for a total of 8 weeks. Followed by 10 weeks with L-cystine (1.2 g/kg), L-methionine (2.2 g/kg) and 2.153 mg isoflavone/g protein with an aglycone component of approximately 1.72 mg/g protein.
host_body_habitat
Gastrointestinal
host_body_product
feces
host_diet
Rats were fed a semi-purified diet similar to the AIN-93G diet, with casein as the protein source for a total of 8 weeks. Followed by a 10 week with L-cystine (1.2 g/kg), L-methionine (2.2 g/kg) and 2.153 mg isoflavone/g protein with an aglycone component of approximately 1.72 mg/g protein. Diet was manufactured by Archer Daniels Midland (AMD) (Decatur, IL, USA)
host_genotype
fa/fa
host_phenotype
obese
host_sex
male
host_subject_id
28
host_taxid
10116
samp_mat_process
Total microbial DNA was collected directly from the fecal samples using a PowerSoil® DNA isolation kit (MoBio Laboratories, Inc., Carlsbad, CA). Isolated DNA was used for amplifying the V4 region of 16S rRNA gene as per Kozich et al. (2013) Data was collected using the Illumina NextSeq 2000.
samp_store_temp
-80 C
source_material_id
feces
illumina_sample_id
S99
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.host-associated