Evolved clone 3, evolved-like bulk
Saccharomyces cerevisiae
To identify the causal mutation that led to increased expression noise in the evolved clones, we performed bulk segregant analysis by crossing the evolved cells with their ancestral cells and analyzing their F1 haploid progeny. We measured protein expression noise levels of both reporter genes in 64 F1 segregants and established an ancestral-like pool and an evolved-like pool from each evolved clone (Evo 2, Evo 3 and Evo 4). For each bulk, 20 progenies exhibiting a consistent phenotype across the three replicates were pooled and subjected to whole genome sequencing analysis.
strain
W303
isolation_source
Laboratory
collection_date
2021-03-18
geo_loc_name
Taiwan
sample_type
Mix of Genomic DNA
BioSampleModel
Microbe, viral or environmental