fli1tp1_24hpf
Danio rerio
Protocols: Dechorionated embryos were collected at defined stages, dissociated by pipetting, centrifugation at 300g for 5 minutes, washed once with PBS, and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter (Bio-Rad). Samples were then re-suspended in 1x PBS with 0.05% BSA at 800-3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4,000 cells. Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4,000 cells. Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v2 or v3 was used for library preparation.
ENA-FIRST-PUBLIC
2023-08-01T00:17:16Z
ENA-LAST-UPDATE
2023-08-01T00:17:16Z
External Id
SAMEA114192246
INSDC center alias
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC center name
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC first public
2023-08-01T00:17:16Z
INSDC last update
2023-08-01T00:17:16Z
INSDC status
public
Submitter Id
E-MTAB-13196:fli1tp1_24hpf
age
24
broker name
ArrayExpress
cell_type
endothelial cell
collection date
not collected
common name
zebrafish
developmental stage
24 hpf
genotype
fli1:DsRed, tp1:eGFP
geographic location (country and/or sea)
not collected
immunophenotype
fli1:DsRed+, tp1:eGFP+
isolate
not applicable
organism part
embryo
sample name
E-MTAB-13196:fli1tp1_24hpf
scientific_name
Danio rerio
strain
fli1:DsRed, tp1:eGFP