H3K9me3 WT IP
Danio rerio
Protocols: WT and kdm4b mutant embryos at 22 hpf were fixed in 1% formaldehyde and then lysed in SDS buffer on ice for 30 minutes. Chromatin was sonicated by Fisher Scientific's Sonic Dismembrator with 30% amplitude, pulse for 10 s on and 30 s off for a total sonication 'on'time of 3 min 30 s to generate 100~500 bp fragment DNA. Sonicated chromatin was precipitated by antibodies specific for H3K9me3 (ab8898, Abcam), H3K36me3 (ab9050, Abcam), FOXO3 (ab12162, Abcam), or Normal Rabbit IgG (Negative control, 2729, CST). 10 ng of DNA samples were prepared for Illumina sequencing as the standard steps. Illumina's genomic adapters were ligated to the DNA fragments. Enriched ligated fragments of ~200 - 500 bp was purified and quantified. The libraries were denatured, captured on Illumina flow cell and amplified in situ.
ENA first public
2024-07-01
External Id
SAMEA113945348
INSDC center name
Shanghai Institute of Nutrition and Diseases, Chinese Academy of Sciences
INSDC last update
2023-06-27T11:02:12Z
INSDC status
public
Submitter Id
E-MTAB-13114:H3K9me3 WT IP
age
22
broker name
ArrayExpress
collection date
not collected
common name
zebrafish
developmental stage
embryo
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
mixed pool of 200 embryos
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-13114:H3K9me3 WT IP
scientific_name
Danio rerio
sex
mixed
strain
Tubingen