MU1
Danio rerio
Protocols: The 48 hpf gata1+ erythrocytes were sorted (FACSAria, BD Bioscience, CA, USA) Two independent groups of RNA from wt and kdm4b-/- erythrocytes were extracted using TRIzol® Reagent (Life Technologies) (each group contains three biological repeats). After adenylation of 3' ends of double strand cDNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase. At last, PCR products were purified and the library quality was assessed on the Agilent Bioanalyzer 2100 system.
ENA first public
2024-07-01
External Id
SAMEA113945411
INSDC center name
Shanghai Institute of Nutrition and Diseases, Chinese Academy of Sciences
INSDC last update
2023-06-27T11:19:32Z
INSDC status
public
Submitter Id
E-MTAB-13116:MU1
age
48
broker name
ArrayExpress
cell_type
erythrocyte
collection date
not collected
common name
zebrafish
developmental stage
embryo stage
disease
normal
genotype
kdm4b -/-
genotype
wild type genotype
geographic location (country and/or sea)
not collected
immunophenotype
gata1 positive
individual
mixed pool of 200 individuals
isolate
not applicable
organism part
embryo
sample name
E-MTAB-13116:MU1
scientific_name
Danio rerio
sex
mixed
strain
Tubingen