Monkeypox whole-genome sequencing from Netherlands 2022
Monkeypox virus
Nanopore sequencing was performed on clinical material after DNAse treatment. In short, DNA was extracted using Ampure Beads after which an end repair step was performed (Ultra II End-prep NEBNext). Adapters were ligated, Ampure bead washes were performed twice and a random PCR was performed (following the SQK-PBK004 protocol from Nanopore).
GISAID Accession ID
EPI_ISL_15269698
INSDC center name
VEO Monkeypox analysis group
Submitter Id
EMC_NL054_mpox
broker name
ERASMUS MC, UNIVERISTY MEDICAL CENTER
collecting institution
VEO Monkeypox analysis group
collection date
2022-05-27
collector name
VEO Monkeypox analysis group
common name
Monkeypox
geographic location (country and/or sea)
Netherlands
geographic location (region and locality)
not collected
host common name
Human
host health state
not collected
host scientific name
Homo sapiens
host sex
not collected
host subject id
restricted access
isolate
hMpxV/Netherlands/un-EMC-NL054/2022
isolation source host-associated
not collected
sample capture status
active surveillance in response to outbreak
sample name
EMC_NL054_mpox
scientific_name
Monkeypox virus