Sample 3
Mus musculus
Protocols: Neutrophils were isolated from bone marrow (BM) cells, and treated with vehicle or IL1b/IL6/PGE2. Then neutrophils were harvested for RNA-seq. Cells were lysed and homogenized in TRIzol Reagent, then RNA was isolated using the miRNeasy Mini kit (Qiagen), according to manufacturers' protocols, including the optional DNase digest step. Libraries were constructed using the KAPA mRNA HyperPrep Kit (Roche Sequencing and Life Science), according to the manufacturer's protocol. Briefly, the protocol entails isolation of polyA containing mRNA using oligo-dT magnetic beads, RNA fragmentation, first and second strand cDNA synthesis, ligation of Illumina-specific adapters containing a unique barcode sequence for each library, and PCR amplification. The quality and concentration of the libraries were assessed using the D5000 ScreenTape (Agilent Technologies) and Qubit dsDNA HS Assay, respectively, according to the manufacturers' instructions.
ENA-FIRST-PUBLIC
2022-12-12
ENA-LAST-UPDATE
2022-12-12
External Id
SAMEA112137649
INSDC center alias
The Jackson Laboratory
INSDC center name
the jackson laboratory
INSDC first public
2022-12-12T00:22:04Z
INSDC last update
2022-12-12T00:22:04Z
INSDC status
public
Submitter Id
E-MTAB-12367:Sample 3
age
10
broker name
ArrayExpress
cell_type
neutrophil
common name
house mouse
genotype
wild type genotype
organism part
bone marrow
sample name
E-MTAB-12367:Sample 3
sex
female
strain
C57BL/6J