PC-9_WWTR1_DMSO_48h_R1
Homo sapiens
Protocols: ~2 million cells per sample were lysed in 350uL of RLT and stored at -80 until subsequent extraction. RNA was extracted using the RNeasy 96 QIAcube HT Kit (Qiagen) with a DNAse digest as per manufactures recommendations. Libraries were prepared using mRNA Stranded library preparation kit (Illumina) and subsequently quantified by Qubit and KAPA library quantification kit, ROX low (Roche). Library sizes were also determined by bioanalyzer.
replicate
1
disease
non-small cell lung adenocarcinoma
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
scientific_name
Homo sapiens
common name
human
organism part
lung
developmental stage
adult
genotype
EGFR mutant
isolate
not applicable
cell_line
PC-9
genetic modification
WWTR1
cell_type
immortal cell line cell
geographic location (country and/or sea)
not collected