WSSS_F_LNG8713191_CS_GEX
Homo sapiens
Protocols: Protocol carried out by HDBR to extract specimen from a fetal donor.  Nucleic acid was extracted according to the standard protocol of the Chromium single cell 5'V1 kit. Libraries were prepared according to the standard protocol of the Chromium 5'V1 VDJ kit. An in-house kit was used for gdTCR enrichment.  V(D)J libraries were prepared according to the manufacturerâ€™s protocol (10X Genomics) using individual Chromium i7 Sample Indices.5,000 PE reads per cell for V(D)J libraries were aimed for sequencing. Libraries for gamma/delta TCR variable regions were amplified as previously described (Mimitou et al. 2019)
disease
normal
individual
HDBR15219
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
male
scientific_name
Homo sapiens
common name
human
organism part
lung
developmental stage
18th week post-fertilization human stage
sampling site
distal lung
isolate
not applicable
protocol
10x 5' v1
age
18
geographic location (country and/or sea)
not collected