nmtDbp2_2_748_t5
Schizosaccharomyces pombe
Protocols: Cells were grown in EMMG, then shifted to YES for 5h or 9h. S. cerevisiae spike-in cells were added at at 1:5 OD600 ratio immediately before harvesting by centrifugation. Total RNA was extracted with the hot phenol method. Libraries were prepared with the VAHTS Stranded mRNA-seq Library Prep Kit (Vazyme Biotech) after poly(A) selection.
strain
YP748
isolate
not applicable
organism
Schizosaccharomyces pombe
organism
Schizosaccharomyces pombe
collection date
not collected
scientific_name
Schizosaccharomyces pombe
common name
fission yeast
organism part
whole organism
genotype
h+ leu1-32 ura4D18 ade6-M216 his3-D1 dbp2::ura4:P.nmt1-dbp2
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2024-01-19