CGD01 V4
Homo sapiens
Protocols: IFN-γ administration was initiated at the dose of 50 mcg/m2 given subcutaneously on the standard schedule of Monday, Wednesday, and Friday in the evening before bedtime. Then, 10-12 hours after the 1st dose, and the 4th dose of IFN-γ additional blood samples were obtained. Neutrophils were isolated from heparinized peripheral blood by Dextran sedimentation, Ficoll-Hypaque density centrifugation, and hypotonic lysis of red blood cells 36,37. Neutrophils were quantitated manually in a hemocytometer in the presence of 3% paraformaldehyde. RNA from neutrophils was isolated by standard technique. RNA was used to prepare libraries using the Universal Plus mRNA-seq kit from Tecan Genomics (Redwood City, CA).
dosing time
Blood draw 10-12 hours after the 4th dose
disease
chronic granulomatous disease
individual
CGD01
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
male
scientific_name
Homo sapiens
common name
human
organism part
blood
compound
IFNG
developmental stage
adult
isolate
not applicable
dose
50
cell_type
neutrophils
age
28.5 YO
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2023-12-22