WSSS_F_LNG8713185_CS_gdTCR
Homo sapiens
Protocols: Protocol carried out by HDBR to extract specimen from a fetal donor.  Nucleic acid was extracted according to the standard protocol of the Chromium single cell 5'V1 kit. V(D)J libraries were prepared according to the manufacturerâ€™s protocol (10X Genomics) using individual Chromium i7 Sample Indices.5,000 PE reads per cell for V(D)J libraries were aimed for sequencing. Libraries for gamma/delta TCR variable regions were amplified as previously described (Mimitou et al. 2019)
disease
normal
individual
HDBR15233
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
male
scientific_name
Homo sapiens
common name
human
organism part
lung
developmental stage
15th week post-fertilization human stage
sampling site
distal lung
isolate
not applicable
protocol
10x TCR enrichment
age
15
geographic location (country and/or sea)
not collected