human gut metagenome
stool sample
ENA-FIRST-PUBLIC
2023-11-07T00:46:01Z
ENA-LAST-UPDATE
2023-11-07T00:46:01Z
External Id
SAMEA112166840
INSDC center name
Max Rubner-Institute
INSDC first public
2023-11-07T00:46:01Z
INSDC last update
2023-11-07T00:46:01Z
INSDC status
public
Submitter Id
Ring007
adapters
fwd:AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGT::rev:CAAGCAGAAGACGGCATACGAGATNNNNNNNNAAGTCGAGAGTCAGTCAGCC
bristol stool index
4
broad-scale environmental context
ENVO_2100002:intestine environment
collection date
2019-12-09
environmental medium
feces
geographic location (country and/or sea)
Germany
geographic location (latitude)
49.0139822
geographic location (longitude)
8.4240071
geographic location (region and locality)
ENVO_01001033:digestive tract environment
host subject id
P4
laboratory id
L2
local environmental context
ENVO_00002003:fecal material
nucleic acid amplification
PCR
nucleic acid extraction
Nucleospin Stool Kit (Macherey Nagel)
pcr conditions
initial denaturation:98degC_2min; 25 cycles: denaturation:98degC_10s; annealing:55degC_30s; extension:72degC_15s; final_extension:72degC_5min
pcr primers
fwd:GTGYCAGCMGCCGCGGTAA::rev:GGACTACNVGGGTWTCTAAT
project name
MRI_Ring
sample name
Ring007
sample volume or weight for DNA extraction
0.2
scientific_name
human gut metagenome
sequencing method
Illumina MiSeq v3
target gene
16S-rRNA
target subfragment
V4