Danio rerio
strain
AB wild type
age
96-120hpf
dev_stage
embryo
collection_date
2022
geo_loc_name
USA: Minnesota, Duluth
sex
not applicable
tissue
whole body zebrafish embryo
biomaterial_provider
US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 56771
breeding_method
Adult zebrafish (AB wild type strain) were housed in an onsite culture facility at the US Environmental Protection Agency (USEPA) laboratory in Duluth, MN. The fish were raised in a flow-through system with control UV-treated, filtered Lake Superior water (LSW) at a flow rate of 250 mL/minute. Fish were held at 26 ± 1°C under a 16/8-hour light/dark photoperiod cycle. Fish were housed in 5.5-gallon tanks with <100 zebrafish per tank. Adult fish were fed live brine shrimp (Artemia) nauplii twice daily. The night prior, breeding zebrafish in groups of four males x six females were separated into breeding tanks containing a plastic screened spawning basket. The next morning, approximately two hours after the lights turned on, embryos were collected and maintained in filtered LSW in an incubator set at 28.5°C. At approximately 4 hours post fertilization (hpf), zebrafish embryos were screened under a stereo microscope, and only similarly staged embryos were sorted out for chemical exposure. All dead, malformed, and unfertilized eggs were discarded.
collected_by
Great Lakes Toxicology & Ecology Division
sample_type
Total RNA
time_code
7
dose
7.38905609893065
dose_units
ug/L
treatment
PFOS (CAS 1763-21-1)
replicate_ID
rep2
repeat_sample
No
BioSampleModel
Model organism or animal