Homo sapiens
A multi-center study was conducted collecting fecal samples (n=37, 29 patients and 8 volunteers) from April 2020 to December 2020 from and microbiome analyses were conducted from cardiac arrest patients
ENA first public
2024-03-20
External Id
SAMEA13566026
INSDC center name
Peking university
INSDC last update
2022-03-20T07:51:49Z
INSDC status
public
Submitter Id
ena-SAMPLE-TAB-20-03-2022-07:51:49:457-35272
collection date
2020-04
common name
human
environment (biome)
animal litter
environment (feature)
human gut
environment (material)
nitrogen cycling
geographic location (country and/or sea)
China
geographic location (latitude)
39.4
geographic location (longitude)
115.7
human gut environmental package
human-gut
investigation type
metagenome
project name
Gut is associated with outcomes in cardiac arrest patients:a retrospective cohort study
sample name
ena-SAMPLE-TAB-20-03-2022-07:51:49:457-35272
scientific_name
Homo sapiens
sequencing method
Gut is associated with outcomes in cardiac arrest patients:a retrospective cohort study sequencing method Fecal matter was used for DNA extraction (QIAamp PowerFecal Pro DNA Kit). DNA library preparation was conducted according to the manufacturer��s instructions using KAPA HyperPlus PCR-free (96rxn), and qualified DNA sequences were divided into fragments of approximately 350 bp. Manufactured dual-indexed adapters with sequences were duplicated in-house. Indexed adapter ligation was performed using an adapter. After ligation, the DNA libraries were purified and amplificated. The libraries were quantified with Qubit2.0 using Q-PCR, and the pool was sequenced with Illumina HiSeq.