Schizosaccharomyces pombe
Protocols: Samples were prepared from 3 biological replicates of mga2Δ (MP815) and mga2Δcbf11Δ (MP836) cells. Cells were cultured to exponential phase in the complex YES medium (with or without ammonium supplementation), 10 mL were harvested (600 g, 2 min) and the cell pellet was flash-frozen. Total RNA was isolated using a hot acidic phenol method followed by phenol-chloroform extractions and precipitation (PMID: 12854975). Extracted RNA was treated with TURBO DNase (Thermo Fisher Scientific) and purified using Qiagen RNeasy columns. RNA quality was assessed on Agilent Bioanalyzer. The sequencing libraries were synthesized using KAPA mRNA HyperPrep Kit (Illumina platform) (Roche, KK8581).
ENA first public
2024-04-05
External Id
SAMEA114307698
INSDC center name
Charles University, Faculty of Science Department of Cell Biology
INSDC last update
2023-08-29T10:12:05Z
INSDC status
public
Submitter Id
E-MTAB-13303:A3
broker name
ArrayExpress
collection date
not collected
common name
fission yeast
genotype
mga2_cbf11
geographic location (country and/or sea)
not collected
growth condition
YES medium
isolate
not applicable
organism part
whole organism
replicate
1
sample name
E-MTAB-13303:A3
scientific_name
Schizosaccharomyces pombe
strain
MP836