Saccharomyces cerevisiae
Protocols: The yeast culture was treated and then centrifuged. Yeast cells (BY4741) were grown at 30°C at 200 r.p.m. in YPD medium. The cells were grown in the presence or absence of lanosterol (LST) at a final concentration of 1 μM. Post-metabolite loading, the cells were either treated or untreated with the α-factor at a final concentration of 30 μM for 2 hours. RNA isolation was done using TRIzol Reagent. The sample was homogenized with TRIzol Reagent, followed by addition of chloroform. The solution was allowed to separate into different layers, and the upper aqueous layer was transferred to isopropanol, in the presence of glycogen for precipitation. The precipitated RNA was washed with ethanol, and resuspended in nuclease-free water. DNAse treatment was done to remove DNA contamination. The pure RNA was again precipitated with ethanol, washed and resuspended in nuclease free water for downstream processing. the nucleic acid library was successfully prepared
ENA first public
2025-01-30
External Id
SAMEA113542862
INSDC center name
Associate Professor The Chemosensory Lab Department of Computational Biology Indraprastha Institute of Information Technology-Delhi (IIIT-Delhi)
INSDC last update
2023-05-23T15:44:46Z
INSDC status
public
Submitter Id
E-MTAB-12992:NC_T2_U1
broker name
ArrayExpress
common name
baker's yeast
genotype
wild type genotype
growth condition
YPD medium at 30 degree Celsius
organism part
whole organism
sample name
E-MTAB-12992:NC_T2_U1
scientific_name
Saccharomyces cerevisiae
strain
BY4741