Streptomyces coelicolor A3(2)
Protocols: For RNA-seq, total RNA was isolated from 30 ml YEME/TSB cultures. Cultures were inoculated with S. coelicolor and cultivated in flasks with spring coils for 24-36h in 30°C. For osmotic stress experiment growth medium was supplemented with 0.5M NaCl. Mycelia were collected at two time points (exponential and early stationary growth, determined individually for each strain based on the growth curve (Fig. S3)) by collecting 2 ml from the culture and centrifugation. Cell pellets were frozen and stored at -70°C for subsequent RNA isolation.  RNA was isolated using the RNeasy Mini Kit (Qiagen) following manufacturer's instructions, DNA digestions was performed using on column digestion with DNase-I (Qiagen) and TURBO DNase I (Ambion). RNA quality and concentration was measured using Nanodrop (Thermo Fisher Scientific) and Qubit (Thermo Fisher Scientific).  The absence of DNA in the sample was confirmed using PCR. Library preparation was performed by Genewiz (Germany).
ENA first public
2024-10-28
INSDC center name
University of Wrocław, Faculty of Biotechnology
INSDC status
public
Submitter Id
E-MTAB-13846:Sample 44
broker name
ArrayExpress
collection date
not collected
developmental stage
exponential
genotype
dhupAhupS
geographic location (country and/or sea)
not collected
growth condition
NaCl suppl. medium
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-13846:Sample 44
scientific_name
Streptomyces coelicolor
serovar
A3(2)