wastewater metagenome
collection_date
2025-01-13
geo_loc_name
USA: Wisconsin
isolation_source
wastewater
ww_population
<100000
ww_sample_duration
24
ww_sample_matrix
raw wastewater
ww_sample_type
composite
purpose_of_ww_sampling
public health surveillance community-level
purpose_of_ww_sequencing
Identification of the diversity of mutations across the SARS-CoV-2 metagenome
sequenced_by
Wisconsin State Laboratory of Hygiene
ww_endog_control_1
pepper mild mottle virus
ww_endog_control_1_conc
170160000
ww_endog_control_1_protocol
IDT PMMoV primer-probe set described in PMID 24056678 measured using dPCR QIAcuity instrument 96W plates (Qiagen).
ww_endog_control_1_units
copies/L
ww_flow
7457261.246
ww_ph
7.27
ww_population_source
population of jurisdiction encompassing the wastewater service area
ww_primary_sludge_retention_time
not applicable
ww_processing_protocol
Internal control spiked in samples at receipt. Samples were concentrated using Nanotrap Microbiome A beads combined with the enhancement reagent 2 (Ceres) on KingFisher Apex and extracted using Maxwell HT viral TNA kit (Promega) on KingFisher Flex.
ww_sample_site
wastewater treatment plant
ww_surv_jurisdiction
WI
ww_surv_system_sample_id
774711001
project_name
Wisconsin SARS-CoV-2 Wastewater Monitoring Network
concentration_method
ceres nanotrap
extraction_method
promega ht tna kit
extraction_control
bovine coronavirus
ww_surv_target_1
SARS-CoV-2
ww_surv_target_1_gene
N gene (N1)
ww_surv_target_1_known_present
yes
ww_surv_target_1_protocol
IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
ww_surv_target_1_conc
218560
ww_surv_target_1_conc_unit
copies/L
ww_surv_target_2
SARS-CoV-2
ww_surv_target_2_gene
N gene (N2)
ww_surv_target_2_known_present
yes
ww_surv_target_2_protocol
IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
ww_surv_target_2_conc
201920
ww_surv_target_2_conc_unit
copies/L
BioSampleModel
SARS-CoV-2: wastewater surveillance