Bos taurus
Protocols: To identify expression quantitative trait loci (eQTL) in Hanwoo cattle, 32 animals were provided by the Hanwoo cattle Research institute, National Institute of Animal Science, South Korea. For RNA-sequencing, tissue samples, such as Abdominal Fat (ABF), Back Fat (BFT) and Kidney Fat (KIF) were collected from the castrated Hanwoo cattle after slaughtering at 10~26 months of age. Ethics approval was obtained from the National Institute of Animal Science (approval no: NIAS20201979). Total RNA was extracted from the trachea with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol. RNA concentration was checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Individual libraries were generated using Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).
phenotype
case
strain
Hanwoo cattle (Korean native cattle)
organism
Bos taurus
organism
Bos taurus
collection date
not collected
sex
castrated male
scientific_name
Bos taurus
common name
cattle
organism part
Abdominal fat; Back fat; Kidney fat
body weight
602
isolate
not applicable
age
26
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2023-10-16