Bacteroides thetaiotaomicron
Protocols: Overnight cultures were mixed with RNAprotect (Qiagen) in the anaerobic chamber to prevent RNA degradation and bacteria were lysed using QIAGEN Proteinase K and TE buffer containing lysozyme. Total RNA was extracted using the Direct Zol kit (Zymo Cat. R2050) according to the manufacturer's instructions and treated with DNase I from the same kit. RNA concentration, quality, and integrity from 4 independent replicates was checked using RNA6000 Nano chips and the Agilent 2100 Bioanalyzer system ribozero plus was used for ribosomal RNA depletion
ENA first public
2024-03-19
External Id
SAMEA114511300
INSDC center name
Institut Pasteur, Biomics Platform
INSDC last update
2023-10-18T15:45:37Z
INSDC status
public
Submitter Id
E-MTAB-13460:delta1847_BSplus5
broker name
ArrayExpress
collection date
not collected
genotype
delta plus
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-13460:delta1847_BSplus5
scientific_name
Bacteroides thetaiotaomicron