Homo sapiens
Protocols: Wash the cells with PBS 1 times. Add the 1x BioSpyder lysis buffer. Incubate at the room temperature for 15 mins. Store the lysates at -80'C until sequencing. Nucleic acid extraction was done following the Tempo-seq protocol detailed here: https://www.frontiersin.org/articles/10.3389/fgene.2018.00485/full#h3 Nucleic acid library construction was done following the Tempo-seq protocol detailed here: https://www.frontiersin.org/articles/10.3389/fgene.2018.00485/full#h3
isolate
not applicable
disease
hepatocellular carcinoma
organism
Homo sapiens
organism
Homo sapiens
cell_line
HepG2
scientific_name
Homo sapiens
common name
human
organism part
liver
developmental stage
juvenile stage
genotype
wild type genotype
ENA-LAST-UPDATE
2022-04-07