wastewater metagenome
collection_date
2025-01-12
geo_loc_name
USA: Wisconsin
isolation_source
wastewater
ww_population
>100000
ww_sample_duration
24
ww_sample_matrix
raw wastewater
ww_sample_type
composite
purpose_of_ww_sampling
public health surveillance community-level
purpose_of_ww_sequencing
Identification of the diversity of mutations across the SARS-CoV-2 metagenome
sequenced_by
Wisconsin State Laboratory of Hygiene
ww_endog_control_1
pepper mild mottle virus
ww_endog_control_1_conc
not provided
ww_endog_control_1_protocol
not provided
ww_endog_control_1_units
not provided
ww_flow
not provided
ww_population_source
population of jurisdiction encompassing the wastewater service area
ww_primary_sludge_retention_time
not applicable
ww_processing_protocol
Samples were concentrated using Nanotrap Microbiome A beads combined with the enhancement reagent 1 (Ceres) and extracted using MagMAX Viral kit (Thermo Fisher Scientific), both on a KingFisher Flex.
ww_sample_site
wastewater treatment plant
ww_surv_jurisdiction
WI
ww_surv_system_sample_id
708
project_name
Wisconsin SARS-CoV-2 Wastewater Monitoring Network
concentration_method
ceres nanotrap
extraction_method
thermo magmax viral/pathogen nucleic acid isolation kit
extraction_control
bovine coronavirus
ww_surv_target_1
SARS-CoV-2
ww_surv_target_1_gene
N gene (N1)
ww_surv_target_1_known_present
not provided
ww_surv_target_1_protocol
GT molecular assay quantified using QIAcuity dPCR 24W (Qiagen)
ww_surv_target_1_conc
not provided
ww_surv_target_1_conc_unit
not provided
ww_surv_target_2
SARS-CoV-2
ww_surv_target_2_gene
N gene (N2)
ww_surv_target_2_known_present
not provided
ww_surv_target_2_protocol
GT molecular assay quantified using QIAcuity dPCR 24W (Qiagen)
ww_surv_target_2_conc
not provided
ww_surv_target_2_conc_unit
not provided
BioSampleModel
SARS-CoV-2: wastewater surveillance