Mus musculus
Protocols: Islets from male mice were isolated at 14-17 weeks of age by injecting 0.6 mg/mL Collagenase P (Roche) into the pancreatic bile duct. Partially dissociated tissue was fractionated using a Histopaque-1077 (Sigma) gradient followed by hand-picking of islets. Islets from individual mice were used for separate RNA sample isolations. All mutant alleles were bred into a C57Black/6J genetic background.  Ascl1Flox (Ascl1tm2Fgu), InsCre (Ins1tm1.1(cre)Thor) and Rosa26tdTomato (Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze) alleles were interbred to create the beta-cell specific Ascl1 knockout (Ascl1betaKO).   Ascl1betaKO and control heterozygous islets were used in the experiment. Mice were either fed regular chow (RC) (4.5% fat content, PicoLab, 5L0D) or HFD (60% fat content, D12492, Research Diets) for 12 weeks.  Ascl1betaKO mice were also bred into homozygous Abcc8 KO (Abcc8tm1.1Mgn) mouse background.   Total RNA was extracted from isolated mouse pancreatic islets using the Maxwell simplyRNA kit (Promega). cDNA libraries were constructed using Illumina library prep kit at Novogene.
INSDC center name
Vanderbilt University
Submitter Id
E-MTAB-13355:AK3
age
12
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
diet
normal diet
genotype
Ascl1Flox/Flox; InsCre/+; Rosa26tdTomato/+
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
islet of Langerhans
sample name
E-MTAB-13355:AK3
scientific_name
Mus musculus
sex
male
strain
C57Black/6J
study group
Ascl1-KO