Schizosaccharomyces pombe
Protocols: Samples were prepared from 3 biological replicates. Two separate experiments were performed: 1) Cells were cultured to exponential phase in the complex YES medium with or without ammonium supplementation. 2) Cells were cultured to exponential phase in the complex YES medium and then treated with DMSO (control) or 20 μM cerulenin for 1 hour. For each sample, 10 mL were harvested (600 g, 2 min) and the cell pellet was flash-frozen. Total RNA was isolated using a hot acidic phenol method followed by phenol-chloroform extractions and precipitation (PMID: 12854975). Extracted RNA was treated with TURBO DNase (Thermo Fisher Scientific) and purified using Qiagen RNeasy columns. RNA quality was assessed on Agilent Bioanalyzer. The sequencing libraries were synthesized using KAPA mRNA HyperPrep Kit (Illumina platform) (Roche, KK8581).
ENA first public
2024-04-05
External Id
SAMEA114307753
INSDC center name
Charles University, Faculty of Science Department of Cell Biology
INSDC last update
2023-08-29T10:43:38Z
INSDC status
public
Submitter Id
E-MTAB-13305:V4
broker name
ArrayExpress
collection date
not collected
common name
fission yeast
genotype
cbf11DBM
geographic location (country and/or sea)
not collected
growth condition
YES medium
isolate
not applicable
organism part
whole organism
replicate
1
sample name
E-MTAB-13305:V4
scientific_name
Schizosaccharomyces pombe
strain
MP712