Photinia x fraseri
Protocols: 10 plants of Photinia x fraseri Dress were planted in pots using commercial soils collected from two different areas of Altopascio (Lucca, Tuscany, Italy). 5 plants were cultivated in a rural area (named \"R\") sparsely inhabited, with significant lower levels of PM (control) compared to 5 plants exposed to higher levels of PM (named \"T\"; “traffic”, close to a traffic light with a high number of moving cars). Plants were irrigated weekly and routinely inspected to determine any eventual symptoms of biotic and abiotic stresses. Plants remain healthy during the three months of the experimental trial. Healthy, fully-expanded, green leaves were sampled from the 5 plants from each zone at the time of planting (T0; 15th March 2021) and three months after planting (T1; 15th June 2021). Leaves were immediately frozen in liquid nitrogen and stored at –80 °C until RNA extraction. Plants have a height of around 1.5 meters and were bought in the same plant nursery to reduce plants' physiological and agronomic variability at the beginning of the trial. Frozen leaves were grounded in liquid nitrogen and a CTAB-modified protocol was used (Doyle & Doyle, 1987) to extract RNA from the tissue powder. The RNAs were eluted in 130 µL of nuclease-free water, treated with DNaseI (NEB) and purified using specific silica-based columns (Monarch RNA Cleanup kit – NEB). Qualitative and quantitative check of the nucleic acid extracted were performed through Agilent 2100 Bioanalyzer (kit RNA nano - Agilent Technologies) and Qubit™ 4 Fluorometer (kit RNA BR - Thermo Fisher Scientific) respectively. Each sample was converted into sequencing libraries following the procedure of Stranded mRNA Library Prep (Illumina) and a combination of exclusive unique dual index. The concentration of each of the libraries was determined using Qubit™ 4 Fluorometer (dsDNA High Sensitivity Kit -Invitrogen).
ENA-FIRST-PUBLIC
2023-03-24
ENA-LAST-UPDATE
2023-03-24
External Id
SAMEA112246051
INSDC center alias
Department of Agriculture, Food, Environment and Forestry (DAGRI) - University of Florence
INSDC center name
Department of Agriculture, Food, Environment and Forestry (DAGRI) - University of Florence
INSDC first public
2023-03-24T07:00:06Z
INSDC last update
2023-03-24T07:00:06Z
INSDC status
public
Submitter Id
E-MTAB-12477:R0_1
age
2
broker name
ArrayExpress
cultivar
Dress
developmental stage
adult
genotype
wild type genotype
growth condition
rural environment (not polluted)
organism part
leaf
sample name
E-MTAB-12477:R0_1
scientific_name
Photinia x fraseri