Homo sapiens
healthy, HIV-1-uninfected
ENA first public
2024-11-16
INSDC center name
WASHINGTON UNIVERSITY SCHOOL OF MEDICINE
INSDC status
public
Submitter Id
23983
broad-scale environmental context
unknown
collection date
2016-02-02
common name
human
environmental medium
stool
geographic location (country and/or sea)
USA
geographic location (latitude)
38.7946
geographic location (longitude)
-106.5348
geographic location (region and locality)
unknown
host age
32
host race
WHITE
host sex
male
host subject id
922
library construction method
16S rDNA sequencing
library reads sequenced
42035
local environmental context
unknown
nucleic acid amplification
PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA, with the reverse PCR primer barcoded with a 12-base error-correcting Golay code to facilitate multiplexing and used to amplify the V4 region of the bacterial 16S gene Caporaso, J.G. et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample, Proc. Natl. Acad. Sci. U.S.A. 108 (supplement_1) 4516-4522, https://doi.org/10.1073/pnas.1000080107 (2011).
nucleic acid extraction
Phenol:chloroform
project name
APPROACH Microbiome
sample name
23983
sample volume or weight for DNA extraction
200 mg stool
scientific_name
Homo sapiens
sequencing method
MiSeq 2 _ 250 paired-end