Mus musculus
Cells were kept in culture for 48h at standard conditions before RNA extractions.
strain
C57BL/6J
isolate
not applicable
breed
Nonagouti (a/a), otherwise wild-type
cultivar
not applicable
ecotype
not applicable
age
not applicable
dev_stage
not applicable
collection_date
2021-07-21
geo_loc_name
Italy: Padova
sex
not applicable
tissue
Cell line was obtained from epidermal melanoblasts from embryos of inbred C57BL mice. Melan-a melanocytes are normal, immortalized murine melanocyte cells derived from C57BL/6J mice. Melan-a melanocytes were obtained from the Wellcome Trust Functional Genomics Cell Bank, St. Georges Hospital, London, UK
biomaterial_provider
Lab Marta Giacomello_Via U. Bassi, 58/B - 35121 Padova
cell_line
melan-a
cell_type
Melanocytes
collected_by
Ana Paula Magalhaes Rebelo_University of Padova
genotype
Pigmented
growth_protocol
Cells were maintained in RPMI-1640 medium supplemented with 10% FBS, 100 U/mL penicillin, 100 g/mL streptomycin, and 200 nM PMA. Cells were kept at 70% confluency between experiments at 37C in a 10% CO2 incubator.
sample_type
Cell culture
counter_column
3
BioSampleModel
Model organism or animal