Mus musculus
Cells were kept in culture for 48h at standard conditions before RNA extractions.
strain
C57BL/6J
isolate
not applicable
breed
Albino (Tyrc/Tyrc)
cultivar
not applicable
ecotype
not applicable
age
not applicable
dev_stage
not applicable
collection_date
2021-07-19
geo_loc_name
Italy: Padova
sex
not applicable
tissue
Melan-c2 melanocytes are immortalized tyrosinase mutant murine melanocytes (mutated Tyrc-2J/Tyrc-2J; a functional null allele) derived from C57BL/6J.
biomaterial_provider
Lab Marta Giacomello_Via U. Bassi, 58/B - 35121 Padova
cell_line
melan-c2
cell_type
Melanocytes
collected_by
Ana Paula Magalhaes Rebelo_University of Padova
genotype
Albino
growth_protocol
Cells were maintained in RPMI-1640 medium supplemented with 10% FBS, 100 U/mL penicillin, 100 g/mL streptomycin, and 200 nM PMA and 2 nM cholera toxin diluted in water (Sigma-Aldrich, St. Louis, MO, Cat. No. C8052). Cell lines were kept at 70% confluency between experiments at 37C in a 10% CO2 incubator.
sample_type
Cell culture
counter_column
5
BioSampleModel
Model organism or animal