Mus musculus
Protocols: Mice were sacrificed and its terminal ileum was collected Total RNA was extracted from the samples taken from colons of mice using the peqGOLD total RNA kit (Peqlab/VWR) following the manufacturers' instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.
ENA first public
2025-01-30
INSDC center name
Universitaetsklinikum Erlangen, Medizin 1
INSDC status
public
Submitter Id
E-MTAB-14325:SI13
age
12
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
dARE
geographic location (country and/or sea)
not collected
individual
13
isolate
not applicable
organism part
ileum
sample name
E-MTAB-14325:SI13
scientific_name
Mus musculus
sex
male
strain
C57BL/6