Homo sapiens
Protocols: Punch skin biopsies of the local area were performed under aseptic conditions and under local anaesthetic (Lidocaine Hydrochloride Injection BP 2%, w/v), using a 6-mm sterile disposable biopsy punch. Following the biopsy, samples were divided with half immediately placed in 10% formalin and transported to the laboratory and the other half placed in RNA laterTM (Fisher Scientific, Loughborough, UK) for bulk RNA sequencing. Control skin samples were obtained from female patients aged 19–82 years, following mastectomy or breast reduction after informed consent. Skin without obvious pathological findings, which was surplus to diagnostic histopathology requirements, was used in the experiments. Control punch biopsy samples were treated in the same manner as described above. After cutting skin samples into small pieces, samples were homogenised and lysed by using Tissue Raptor II and QIAzol® Lysis protocol (QIAzol® Handbook 2021, QIAgen, Crawley, UK). The quality of RNA was routinely assessed by determining the A260:A280 ratio using NanoDrop2000 (Thermo Scientific, UK). RNA libraries were created using TruSeq stranded Total RNA with ribozero GOLD (illumina, UK)
ENA-FIRST-PUBLIC
2023-10-06T15:00:31Z
ENA-LAST-UPDATE
2023-10-06T15:00:31Z
External Id
SAMEA114461635
INSDC center name
CARDIFF
INSDC first public
2023-10-06T15:00:31Z
INSDC last update
2023-10-06T15:00:31Z
INSDC status
public
Submitter Id
E-MTAB-13386:Control 2
age
19 to 82
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
individual
Control 2
isolate
not applicable
organism part
skin
sample name
E-MTAB-13386:Control 2
scientific_name
Homo sapiens
sex
female