freshwater metagenome
isolation_source
Lotus Pond
collection_date
2024-08-22
geo_loc_name
China:Beijing
lat_lon
39.92 N 116.38 E
samp_mat_process
The Lotus Pond water were used for metagenomic sequencing and analysis. Briefly, the sediments scratched from the filter membranes were digested with Proteinase K and RNase A, and genomic DNA fragments were extracted with magnetic beads. A biomass of 1 g genomic DNA was fragmented and fragments with lengths varied from 200 bp to 400 bp were selected for end-repaired, 3 adenylation and ligation to adaptors. The resulted fragments were used for product amplification and circularization, and the uncyclized linear DNA molecules were digested.
BioSampleModel
Metagenome or environmental