Mus musculus
Protocols: Around 1x105 SSCs (cultured in medium 1 for 7 passages), Intermediate state cells (SSCs cultured in medium 1 for 5 passages and further cultured in medium 2 for 2 passages), GSPCs (SSCs cultured in medium 1 for 5 passages and further cultured in medium 2 for 5 passages) and ES-like cells (10 passages) were collected using the identical protocols. Total RNA was extracted using Trizol (Ambion Life Technologies) according to the Ambion standard RNA isolation procedure. mRNA was purified using the NEBNext Poly (A) mRNA Magnetic Isolation Beads (NEB, USA). Then the mRNA library was constructed with a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420S/L, NEB).
strain
ICR
isolate
not applicable
organism
Mus musculus
organism
Mus musculus
growth condition
GSPCs medium
scientific_name
Mus musculus
common name
house mouse
cell_type
germline stem cell derived pluripotent cell
age
2 to 3
developmental stage
mouse postnatal
genotype
wild type genotype
ENA-LAST-UPDATE
2022-10-28