pig gut metagenome
Small intestine_After_Zinc_4611
ENA-FIRST-PUBLIC
2022-12-09
ENA-LAST-UPDATE
2022-12-09
External Id
SAMEA7703632
INSDC center alias
QUEEN'S UNIVERSITY BELFAST
INSDC center name
QUEEN'S UNIVERSITY BELFAST
INSDC first public
2022-12-09T00:31:33Z
INSDC last update
2022-12-09T00:31:33Z
INSDC status
public
Submitter Id
42
adapters
Illumina adapters
collection date
2020
common name
pig
environment (biome)
swine
environment (feature)
GIT
environment (material)
Small intestine
geographic location (country and/or sea)
United Kingdom
geographic location (latitude)
54.579.058
geographic location (longitude)
-5.936.998
geographic location (region and locality)
Belfast
host common name
pig
host diet
Zinc
host-associated environmental package
host-associated
investigation type
bacteria_archaea
nucleic acid amplification
PCR
nucleic acid extraction
Fast DNA SPIN Kit for Soil
pcr conditions
95�C for 3 minutes, 20 cycles at 95�C for 30 seconds, 55�C for 30 seconds, 72�C for 30 seconds and then 72�C for 5 minutes.
pcr primers
515F and 806R
project name
Affect_of_zinc_and_alternative_compound_on pigs'_microbiota
sample name
42
sample storage temperature
-80
sequencing method
Illumina MiSeq
target gene
16S: V4- 250 bp