mouse gut metagenome
Chemotheray treatment, mouse 500
collection_date
2020-05-18
env_broad_scale
gut(UBERON:0001007)
env_local_scale
colon(UBERON:0001155)
env_medium
feces(UBERON:0001988)
geo_loc_name
USA:Arkansas,Little Rock (GAZ,00165676)
host
Mus musculus (C57/BL6)
lat_lon
34.7504 N 92.3227 W
chem_administration
60 mg/kg cyclophosphamide, 4 mg/kg methotrexate, and 60 mg/kg fluorouracil
host_body_habitat
Gastrointestinal
host_body_product
feces
host_diet
ad libitum availability to drinking water and standard chow
host_last_meal
standard chow
host_life_stage
adult
host_sex
female
host_subject_id
500
host_taxid
10090
perturbation
Intraperitoneal injections on days 1, 8, 15, and 22 with 60 mg/kg cyclophosphamide, 4 mg/kg methotrexate, and 60 mg/kg fluorouracil
samp_mat_process
Fecal samples were collected from individual mice and stored at -80C. Fecal pellets were shipped to RTL Genomics (Lubbock, Texas) for DNA extraction and 16S rRNA gene sequencing using their "MiSeq Diversity Assay" targeting the V3V4 hypervariable region, with the primers (357wF: CCTACGGGNGGCWGCAG - 785R: GACTACHVGGGTATCTAATCC).
samp_store_temp
-80 C
amplicon_region
V3V4
forward_primer
CCTACGGGNGGCWGCAG
reverse_primer
GACTACHVGGGTATCTAATCC
sequencing_provider
V3V4 16S rRNA gene amplicon sequences were provided by RTL Genomics (Lubbock, Texas).
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.host-associated