Vaccinia virus Lister
Protocols: Virus particles were partially purified from lysates by sucrose gradient centrifugation. RK13 cells were maintained in DMEM + 5% FBS + gentamicin. 48 hours prior to infection, RK13 cells were seeded into 6 well plates at (500000 cells/well). Confluent RK13 cells were infected at MOI = 0.2 (2.40E+05 pfu) Viral genomic DNA was isolated using standard published methods. PacBio CCS
ENA-FIRST-PUBLIC
2022-09-22
ENA-LAST-UPDATE
2022-09-22
External Id
SAMEA7473244
INSDC center alias
UNIVERSITY OF CALIFORNIA DAVIS
INSDC center name
UNIVERSITY OF CALIFORNIA DAVIS
INSDC first public
2022-09-22T04:31:26Z
INSDC last update
2022-09-22T04:31:26Z
INSDC status
public
Submitter Id
E-MTAB-9682:Sample 11
broker name
ArrayExpress
genotype
VC-R2 that lacks both K3L and E3L and expresses EGFP
passage
0
sample name
E-MTAB-9682:Sample 11