Mus musculus
Protocols: 3-, 12- and 18-month-old C57BL/6 male (Janvier) mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10 mg of human TNFR2 protein (Peprotech, 310-12) and 20 mg MPLA (Sigma, L6895) adjuvant in 150 mL PBS. All samples are from mice immunized with TNFR2 protein. Cells were isolated via facs following the aforementioned experimental conditions 10x genomics 5' protocol was performed as according to manufacturers 10x genomics 5' protocol was performed as according to manufacturers
INSDC center name
ETH Zurich
Submitter Id
E-MTAB-12610:Sample 21_VDJ
age
72
broker name
ArrayExpress
cell_type
B cell
cohort
18m SP
collection date
not collected
common name
house mouse
developmental stage
adult
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
spleen
sample name
E-MTAB-12610:Sample 21_VDJ
scientific_name
Mus musculus
sex
male
strain
C57BL/6J