Mus musculus
Protocols: Cells were harvested by trypsinization, washed with PBS and mixed in RNA lysis buffer (Zymo Research). Cardiac tissue was lysed in 600 µL of RNA lysis buffer (Zymo) and homogenized using TissueLyserII (Qiagen). Homogenate was spun down for 2 min at 15000 g and the supernatant was processed according to the manufacturer's instructions. Zymo Quick-RNA MicroPrep isolation kit was used to extract total RNA. 100% ethanol was added to the lysates and samples were than processed on the columns. DNA contamination was removed by DNase I digestion. RNA was washed and eluted in 20 µl of water. RNA concentration and purity were assessed with Nanodrop 1000 (Thermo Fisher Scientific) RNA-seq library preparation workflow Figure 2.2.1 Strand-specific RNA sequencing with polyA selection.
ENA-FIRST-PUBLIC
2022-10-27
ENA-LAST-UPDATE
2022-10-27
External Id
SAMEA111479029
INSDC center alias
Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich
INSDC center name
Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich
INSDC first public
2022-10-27T00:17:18Z
INSDC last update
2022-10-27T00:17:18Z
INSDC status
public
Submitter Id
E-MTAB-12271:Sample 7
age
16
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
disease
rheumatic disease
genotype
Fosl-2
organism part
heart
sample name
E-MTAB-12271:Sample 7
strain
BTBR mouse