Homo sapiens
Protocols: Skin suction blisters were raised: Suction cups were applied to the deltoid region of participants' arms, at the site of previous injection. Skin suction blisters were performed by gradually applying negative pressure (up to 60 kPa) from a suction pump machine VP25 (Eschmann, Lancing, UK) through a suction blister cup with a 15-mm hole in the base (UHBristol NHS Foundation Trust Medical Engineering Department, Bristol, UK) for 2–4 hr until a unilocular blister had formed within the cup. The cup was left in place for a further 30-60 minutes to encourage migration of lymphocytes into the blister fluid. The cup was then removed, and the blister fluid aspirated using a needle and syringe. Blister fluid was immediately suspended in 10ml heparinised RPMI (Gibco)+ 5% foetal calf serum Cells were counted then washed once and resuspended in an appropriate volume for scRNAseq. Samples were used fresh on the day of the collection. Samples were processed using a 10xGenomics V1 human 5'GEX kit libraries were constructed following standard 10xGenomics protocols.
ENA-FIRST-PUBLIC
2023-10-06T15:00:08Z
ENA-LAST-UPDATE
2023-10-06T15:00:08Z
External Id
SAMEA114462826
INSDC center name
CARDIFF
INSDC first public
2023-10-06T15:00:08Z
INSDC last update
2023-10-06T15:00:08Z
INSDC status
public
Submitter Id
E-MTAB-13388:EEASI-A GEX
age
29
broker name
ArrayExpress
cell_type
lymphocyte
collection date
not collected
common name
human
developmental stage
adult
disease
autoimmune type 1 diabetes
geographic location (country and/or sea)
not collected
individual
yes
isolate
not applicable
organism part
zone of skin
protocol
GEX
sample name
E-MTAB-13388:EEASI-A GEX
scientific_name
Homo sapiens
sex
male