TempO-Seq of larval Pimephales promelas whole body after 24 h exposure to 8:2FTS (DTXSID00192353)
Pimephales promelas
strain
GLTED-wt
age
5-6 days post-fertilization
dev_stage
larval
collection_date
2021-11/2022-01
geo_loc_name
USA: Minnesota, Duluth
sex
not determined
tissue
whole body
biomaterial_provider
US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804
breeding_method
All larval fathead minnows (Pimephales promelas) used for testing were provided by an on-site aquatic culture facility at the US EPA Great Lakes Toxicology and Ecology Division, Duluth, MN. Five days in advance of each exposure, newly fertilized fathead minnow embryos, from several breeding pairs, were gently rolled off polyvinyl chloride breeding tiles and transferred to a bath of aerated Lake Superior water and held at 25 C. On day five post-fertilization, fish that had hatched and were free swimming were pooled for use in the assay. All procedures involving animals were approved by a local animal care and use committee.
collected_by
Great Lakes Toxicology & Ecology Division
sample_type
Whole body homogenate
store_cond
-80 deg C
exposure_duration
24 h
nominal conc (uM)
0.1
measured conc
13
conc_units
ug/L
treatment
8:2FTS (DTXSID00192353)
replicate_ID
pool3
BioSampleModel
Model organism or animal