binned metagenome: spire_mag_02696138
Bacteroides uniformis
ENA first public
2024-09-20
INSDC center name
EMBL Heidelberg
INSDC status
public
Submitter Id
spire_mag_02696138
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
megahit 1.2.9
binning parameters
default
binning software
metabat 2.12.1
broad-scale environmental context
human digestive system
broker name
EBI-EMG
collection date
missing: third party data
completeness score
93.19
completeness software
checkm2 0.1.3
contamination score
0.26
environmental medium
not provided
geographic location (country and/or sea)
not provided
geographic location (latitude)
missing: third party data
geographic location (longitude)
missing: third party data
investigation type
metagenome-assembled genome
isolation_source
human gut metagenome
local environmental context
human gut
metagenomic source
human gut metagenome
project name
Objective: Despite the association of nonalcoholic fatty liver disease (NAFLD) with obesity, NAFLD is also found in non-obese individuals. Few studies have focused on the role of the gut microbiome in non-obese NAFLD. We aimed to compare gut microbiome profiles between non-obese and obese NAFLD subjects.Design: The gut microbiome profiles of 171 Asian subjects with biopsy-proven NAFLD and 31 metabolically healthy controls were analyzed using 16S rRNA sequencing. Study subjects were divided into non-obese (n=64) and obese (n=138) groups. Each group was classified into three subgroups according to fibrosis severity (stage, 0 vs. 1 vs. 2–4). The microbiome profiles were compared with clinical metadata, including metabolic traits and genetic variants.Results: Significant alterations in gut microbiota according to fibrosis severity were observed only in non-obese subjects. Specifically, Enterobacteriaceae, Veilloneallaceae, and other gram-negative bacteria were gradually enriched with increasing fibrosis severity. Ruminococcaceae, however, was depleted with significant fibrosis (≥F2). Significant associations between these bacteria and serum free fatty acid, adipose tissue insulin resistance, and glycosylated hemoglobin were found only in non-obese subjects. Apparent and strong co-occurring/co-excluding interactions were shown in non-obese subjects. Particularly, the addition of microbial abundance of Enterobacteriaceae, Veilloneallaceae, and Ruminococcaceae to body mass index and fibrosis 4 index showed 0.953 of area under the receiver-operating characteristic curvefor predicting significant fibrosis in non-obese NAFLD subjects.Conclusion: Our results demonstrated disparate compositions in gut microbiota between obese and non-obese Asian NAFLD subjects according to fibrosis severity, highlighting the pathogenic role of gut microbiota, especially in non-obese NAFLD.
sample derived from
SAMEA6827975
sample name
spire_mag_02696138
scientific_name
Bacteroides uniformis
sequencing method
NextSeq 550
taxonomic identity marker
multi-marker approach