Seeds were sterilized in ethanol (70%), and sodium hypochlorite (50%) and rinsed five times in sterile water, followed by germination of dehulled seeds in Petri dishes (50 mm, and covered in two layers of aluminium foil to keep dark) in growth chamber under  constant temperature (about 18°C), humidity (about 75% relative humidity), 16 hours day length. Parts of the coleoptile, mesocotyl and seminal roots were dissected from germinating seeds starting from 4 days after germination and promptly frozen in liquid nitrogen and stored at -80˚C before thawing prior to RNA extractions.
Avena byzantina
material source longitude
not collected
organism
Avena byzantina
biological material ID
USDA:PI 258586
collection date
not collected
sample ID
IPK_LIMS:3358614
biological material longitude
not collected
biological material ploidy
hexaploid
material source geographic location
not collected
checklist
BSDC00002
sample description
Seeds were sterilized in ethanol (70%), and sodium hypochlorite (50%) and rinsed five times in sterile water, followed by germination of dehulled seeds in Petri dishes (50 mm, and covered in two layers of aluminium foil to keep dark) in growth chamber under constant temperature (about 18°C), humidity (about 75% relative humidity), 16 hours day length. Parts of the coleoptile, mesocotyl and seminal roots were dissected from germinating seeds starting from 4 days after germination and promptly frozen in liquid nitrogen and stored at -80˚C before thawing prior to RNA extractions.
biological material latitude
not collected
growth facility
greenhouse
material source altitude
not collected
material source description
not collected
project name
PanOat Transcriptome
genus
Avena
species
byzantina
plant structure development stage
not provided
material source latitude
not collected
library type
RNAseq
biological material geographic location
not collected
accession name
Bizantina 956
geographic location (country and/or sea)
not collected
plant anatomical entity
embryo