Metagenome-assembled genome: SRR15604481_bin.9_metawrap_v1.3_MAG
uncultured Clostridium sp.
ENA-FIRST-PUBLIC
2022-12-13
ENA-LAST-UPDATE
2022-12-13
External Id
SAMEA112229087
INSDC center alias
EMG
INSDC center name
EMG
INSDC first public
2022-12-13T16:27:13Z
INSDC last update
2022-12-13T16:27:13Z
INSDC status
public
Submitter Id
SRR15604481_bin.9_metawrap_v1.3_MAG
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
not provided
binning parameters
MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
binning software
metawrap v1.3
broad-scale environmental context
mouse digestive system
broker name
EMG broker account, EMBL-EBI
collection date
2019-11-18
completeness score
73.68
completeness software
CheckM
contamination score
0.0
environmental medium
feces
geographic location (country and/or sea)
China
geographic location (latitude)
40.51
geographic location (longitude)
110.141
investigation type
metagenome-assembled genome
isolation_source
mouse gut metagenome
local environmental context
digestive tube
metagenomic source
mouse gut metagenome
project name
To evaluate the role of probiotics in restoring the antibiotic-destroyed gut microbiota and efficacy of ICB therapies, we studied the effect of Lactobacillus rhamnosus Probio-M9 combined with anti-PD-1 on mouse colon cancer and the dynamic changes of gut microbiome by metagenome analyses. The results showed that the combination of Probio-M9 significantly improved tumor inhibition compared with ICB therapy alone in antibiotics treated mice, which could be attribute to the upregulated microbial diversity and increased abundance of beneficial bacteria such as Bifidobacterium pseudolongum, Parabacteroides distasonis and some species of Bacteroides after Probio-M9 supplemented. These changes of structure and composition in gut microbiota caused the alternation of microbiome function, of which the sugar degradation, vitamin and amino acid synthesis pathways were significantly enriched. Collectively, this study indicated that Probio-M9 enhanced the host&apos;s immunotherapeutic response by promoting the colonization of beneficial bacteria and improving intestinal function.
sample derived from
SAMN20968483
sample name
SRR15604481_bin.9_metawrap_v1.3_MAG
sequencing method
Illumina HiSeq 2500
taxonomic classification
The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium;s__
taxonomic identity marker
multi-marker approach