Hashed_eGBOs_1mo
Homo sapiens
source_name
Dissociated whole brain organoids
tissue
Dissociated whole brain organoids
cell line
H1 hESC
treatment
Approximately ten organoids per condition were pooled and dissociated in TrypLE for 15min with gentle agitation every 5min. Single cell solutions were resuspended in 200µL of cell staining buffer (BioLegend, 420201) plus 1µg of TotalSeq-A hashtag antibodies (BioLegend, 399907) and incubated for 30min on ice. After three washes in 1mL of cell staining buffer, cells were resuspended in DMEM at a concentration of 1000cells/µL and pooled together.
geo_loc_name
missing
collection_date
missing