binned metagenome: spire_mag_02534691
Bacteroides uniformis
ENA first public
2024-09-20
INSDC center name
EMBL Heidelberg
INSDC status
public
Submitter Id
spire_mag_02534691
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
megahit 1.2.9
binning parameters
default
binning software
metabat 2.12.1
broad-scale environmental context
human digestive system
broker name
EBI-EMG
collection date
2017
completeness score
78.26
completeness software
checkm2 0.1.3
contamination score
6.15
environmental medium
stool
geographic location (country and/or sea)
Netherlands
geographic location (latitude)
52.29398138
geographic location (longitude)
4.95841566
investigation type
metagenome-assembled genome
isolation_source
human gut metagenome
local environmental context
human gut
metagenomic source
human gut metagenome
project name
Background: Recent studies demonstrate that a Mediterranean diet has beneficial metabolic effects in metabolic syndrome subjects. Since we have shown that fecal microbiota transplantation (FMT) from lean donors exerts beneficial effects on insulin-sensitivity, in the present trial we investigated the potential synergistic effects on insulin-sensitivity of combining Mediterranean diet with donor FMT in subjects with metabolic syndrome.Design: Twenty-four male subjects with metabolic syndrome were put on a Mediterranean diet and after a two-week run-in phase, subjects were randomized to either lean donor (n=12) or autologous (n=12) FMT. Changes in gut microbiota composition and bacterial strain engraftment after 2-week dietary regimens and 6 weeks post-FMT were primary endpoints. Secondary objectives were changes in glucose fluxes (both hepatic and peripheral insulin sensitivity), postprandial plasma incretin (GLP-1) levels, subcutaneous adipose tissue inflammation and plasma metabolites.Results: Consumption of the Mediterranean diet resulted in a reduction in body weight, HOMA-IR and lipid levels. However, no large synergistic effects of combining the diet with lean donor FMT were seen on gut microbiota diversity after 6 weeks. Although we did observe changes in specific bacterial species and plasma metabolites, no significant beneficial effects on glucose fluxes, postprandial incretins or subcutaneous adipose tissue inflammation were detected.Conclusions: In this small pilot randomized controlled trial, no synergistic beneficial metabolic effects of combining Mediterranean diet with lean donor FMT on glucose metabolism were achieved. However, we observed engraftment of specific bacterial species. Future trials are warranted to test the combination of other microbial interventions and diets in metabolic syndrome.
sample derived from
SAMEA8690996
sample name
spire_mag_02534691
scientific_name
Bacteroides uniformis
sequencing method
Illumina NovaSeq 6000
taxonomic identity marker
multi-marker approach