M03T0
gut metagenome
Protocols: Fecal samples were collected at baseline, after 12 weeks and 6 months of lifestyle intervention in tubes filled with DNA stabilization buffer (CANVAX Biotech). For 16S rRNA sequencing, the total microbial DNA was extracted using the QIAamp PowerFecal DNA Kit (Qiagen) following the manufacturer's protocol. The Illumina 16S Metagenomic Sequencing Library Preparation for high-throughput sequencing was performed as follows: 12.5 ng of each DNA extract was employed for the amplification of the V3-V4 hypervariable regions of the bacterial 16S ribosomal RNA (rRNA) gene, using the universal primers previously published by Klindworth et al. with Illumina adapters.
ENA-FIRST-PUBLIC
2023-11-28T00:25:27Z
ENA-LAST-UPDATE
2023-11-28T00:25:27Z
External Id
SAMEA112322270
INSDC center name
Cancer Stem Cells Unit, ISBReMIT, IRCSS Casa Sollievo della Sofferenza, Opera di San Pio da Pietrelcina
INSDC first public
2023-11-28T00:25:27Z
INSDC last update
2023-11-28T00:25:27Z
INSDC status
public
Submitter Id
E-MTAB-12486:M03T0
broker name
ArrayExpress
clinical history
Homo sapiens hospitalised patient
disease
breast cancer
organism part
feces
sample name
E-MTAB-12486:M03T0
scientific_name
gut metagenome