B16-F10_Trex1 KO_1
Mus musculus
Protocols: Cultured cell lines were spun into cell pellets were snap frozen. Snap frozen cell pellets from cultured cell lines were directly lysed with RLT lysis buffer of the Rneasy Plus Mini kit (Qiagen).  Total RNA was quantified with the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and quality was assessed using RNA ScreenTape on a TapeStation 4200 (Agilent Technologies). For sequencing library generation, the TruseqStranded mRNA kit (Illumina) was used with an input of 100-1000 nanograms of total RNA. Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies).
isolate
not applicable
organism
Mus musculus
organism
Mus musculus
cell_line
B16-F10
collection date
not collected
scientific_name
Mus musculus
common name
house mouse
genotype
Trex1 KO
geographic location (country and/or sea)
not collected