Op_803_5
Caenorhabditis elegans
Protocols: Twenty young adult animals were picked to the fresh plates and allowed to lay eggs for 2 hours, after which the adult animals were removed and eggs were allowed to develop into L4 stage. Ten plates of L4 animals were washed off and placed on either OP50 or V. cholerae containing plates for 8 hours. Subsequently, the animals were collected and rinsed three times with M9 buffer. After washing, the worm pellet was frozen in liquid nitrogen. Animals were homogenized using Bullet Blender (Next Advance) in the presence of Qiazol Lysis Reagent and 0.5mm Zirconia beads at 4°C. RNA was prepared using RNeasy Plus Universal Mini Kit (Qiagen). Library was constructed and sequenced by Novogene
isolate
not applicable
individual
mixed
organism
Caenorhabditis elegans
organism
Caenorhabditis elegans
collection date
not collected
sex
mixed
scientific_name
Caenorhabditis elegans
organism part
whole organism
developmental stage
L4 larva
genotype
shk-1 KO
geographic location (country and/or sea)
not collected
infect
none