L-mg-1
Saccharomyces cerevisiae
Protocols: Cultured yeast cells were used to isolate mitochondria using a Yeast Mitochondria Isolation Kit (Abcam). mtDNA was extracted from isolated mitochondria using the FastGene Plasmid Mini Kit (Nippon Genetics, Tokyo, Japan) and purified using AMpure XP beads (Beckman Coulter, CA, USA). Extracted gDNA and mtDNA were subjected to library construction using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA).
strain
S288c
isolate
not applicable
organism
Saccharomyces cerevisiae
organism
Saccharomyces cerevisiae
growth condition
SDC+A, YPG (72, 48 hours)
collection date
not collected
scientific_name
Saccharomyces cerevisiae
common name
brewer's yeast
organism part
whole organism
fraction
mitochondrial fraction
genotype
CEN.PK2-1C (Euroscarf: 30009A) pMnC-CDA gRNA library
geographic location (country and/or sea)
not collected
ENA-LAST-UPDATE
2024-01-18