OX-IP_R4
Arabidopsis thaliana
Protocols: Plants grown in MS plates in continuous light (LL) for 12 days were vacuum-infiltrated with 1% formaldehyde for 15 min followed by quenching with 125 mM glycine. A whole-cell extract was prepared in RIP-lysis buffer. The extract was pre-cleared with Sepharose beads and subjected to immunoprecipitation with GFP-Trap beads (Chromotek). After extensive washing with RIP washing buffer, co-precipitated RNAs were extracted with Trizol (Invitrogen) and treated with DNase (Promega). RNAs were extracted with Trizol (Invitrogen) and treated with DNase (Promega). Libraries were prepared by genome center at the Max Planck-Genome-Centre Cologne (MP-GC).
ENA-FIRST-PUBLIC
2023-05-31
ENA-LAST-UPDATE
2023-05-31
External Id
SAMEA112139659
INSDC center alias
Department of Biochemistry, University of Otago
INSDC center name
Department of Biochemistry, University of Otago
INSDC first public
2023-05-31T00:15:38Z
INSDC last update
2023-05-31T00:15:38Z
INSDC status
public
Submitter Id
E-MTAB-12369:OX-IP_R4
age
12
broker name
ArrayExpress
common name
thale cress
developmental stage
seedling development stage
genotype
35S-YFP
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-12369:OX-IP_R4
scientific_name
Arabidopsis thaliana
ecotype
Columbia