Trp53−/− 2
Mus musculus
Protocols: Fetal liver from embryos from timed crossings of mating pairs of appropriate genotypes were harvested 14 days after detection of plugs (E14.5). Pregnant females were euthanized and they embryos extracted in ice-cold PBS. Fetal livers were collected in PBS and dissolved by pipetting and small aliquot was used for preparation of RNA in Trizol reagent within 1 hour after extraction of mouse embryos. Total RNA from freshly harvested fetal livers was isolated with Trizol reagent following manufacturer's protocol. 1µg of DNase I-treated RNA isolated from treated cells was used for poly-A mRNA isolation with the NEBNext Poly(A) mRNA Magnetic Isolation Module. Paired-end libraries were prepared using the NEBNext Ultra II Directional RNA Library Kit with NEBNext multiplex index oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs).
ENA-FIRST-PUBLIC
2023-05-31
ENA-LAST-UPDATE
2023-05-31
External Id
SAMEA112228600
INSDC center alias
Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV); First Faculty of Medicine; Charles University, Czech Republic
INSDC center name
Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV); First Faculty of Medicine; Charles University, Czech Republic
INSDC first public
2023-05-31T00:15:39Z
INSDC last update
2023-05-31T00:15:39Z
INSDC status
public
Submitter Id
E-MTAB-12448:Trp53−/− 2
age
embryonic day 14.5
broker name
ArrayExpress
common name
house mouse
developmental stage
embryo
disease
normal
genotype
Trp53−/−
individual
Q4370-E7
isolate
not applicable
organism part
liver
sample name
E-MTAB-12448:Trp53−/− 2
scientific_name
Mus musculus
strain
C57BL/6J