JD02
Streptomyces venezuelae
Protocols: RNA was isolated from Streptomyces venezeulae JD01 (cobbB1 deletion) or JD07 (cobB2 deletion) strains cultivated in 5 mL MYM liquid medium for 10 or 20 hours (each time point in duplicate). Mycelia were collected by centrifugation, frozen, and stored at -70°C for subsequent RNA isolation. RNA from mycelia was isolated using the GeneJET RNA isolation kit (Thermo Fisher Scientific, US). The lysozyme concentration in the suspension buffer was increased up to 10 mg/mL to improve the yield of mycelium lysis. The isolated RNA was digested with TURBO DNase I (Invitrogen, United States) and subsequently, purified and concentrated using a GeneJET RNA Cleanup kit (Thermo Fischer Scientific, US) RNA libraries were prepared for sequencing using standard Illumina protocols (NEBNext Ultra II Directional RNA Library Prep Kit). rRNA was removed using Ribo-Zero Ribosomal RNA Removal Kit (Illumina)
strain
JD01
isolate
not applicable
replicate
2
organism
Streptomyces venezuelae
organism
Streptomyces venezuelae
collection date
not collected
scientific_name
Streptomyces venezuelae
organism part
whole organism
developmental stage
vegetative
geographic location (country and/or sea)
not collected