WT_1
Mus musculus
Protocols: FACS purified P14 spermatogonial stem cells DNA from spermatogonia was isolated by proteinase K digest (10 mM Tris-HCl pH 8, 5 mM EDTA , 1% SDS, 0.3 M Na-acetate, 0.2 mg/ml proteinase K) overnight, followed by 2x phenol/chloroform/isoamylalcohol (25:24:1, Sigma-Aldrich) extraction and 1x chloroform extraction. The DNA was precipitated at -20˚C after addition of 1/10 volume 3M Na-acetate, 10µg linear acrylamide (Invitrogen) and 1 volume of isopropanol, washed 2x and solubilized in 5mM Tris pH8. Methyl-seq libraries were prepared using the NEBnext Enzymatic Methyl-seq kit (NEB) according to the manufacturer's instructions
ENA first public
2024-07-22
External Id
SAMEA112849610
INSDC center name
Oxford University IDRM, Department of Paediatrics
INSDC last update
2023-03-29T13:58:28Z
INSDC status
public
Submitter Id
E-MTAB-12713:WT_1
broker name
ArrayExpress
common name
house mouse
developmental stage
juvenile stage
genotype
wild type genotype
isolate
not applicable
organism part
testis
sample name
E-MTAB-12713:WT_1
scientific_name
Mus musculus
sex
male
strain
C57BL/6N