HCC1806 siRNF40.2_H3K79me3
Homo sapiens
Protocols: HCC1806 cells were grown in RPMI GlutaMax medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37 C and 5% CO2 siRNA transfections were performed using Lipofectamine RNAiMAX(Invitrogen) according to the manufacturer's instructions Chromatin immunoprecipitation was performed 48 hrs after transfection using antibodies against H2Bub1 (Cat. No. 5546 S, Cell Signaling Technology), H3K79me3 (Cat. No. C15410068, Diagenode), H3K27ac (Cat. No. C15410196, Diagenode). Next-generation sequencing library was prepared using the KAPA Hyper Prep Kit (KR0961–v6.17) according to the manufacturer's instructions and samples were sequenced (single-end 50 bp).
ENA-FIRST-PUBLIC
2023-07-20T00:18:59Z
ENA-LAST-UPDATE
2023-07-20T00:18:59Z
External Id
SAMEA110418563
INSDC center alias
University Medical Center Gottingen Department of Gynecology and Obstetrics Molecular Gynecology
INSDC center name
University Medical Center Gottingen Department of Gynecology and Obstetrics Molecular Gynecology
INSDC first public
2023-07-20T00:18:59Z
INSDC last update
2023-07-20T00:18:59Z
INSDC status
public
Submitter Id
E-MTAB-12000:HCC1806 siRNF40.2_H3K79me3
broker name
ArrayExpress
cell_line
HCC1806
common name
human
developmental stage
adult
disease
triple-negative breast cancer
genotype
wild type genotype
isolate
not applicable
organism part
mammary gland
replicate
2
sample name
E-MTAB-12000:HCC1806 siRNF40.2_H3K79me3
scientific_name
Homo sapiens