Rps19 R67∆/R67∆ 1
Mus musculus
Protocols: A single cell suspension of bone marrow from 6 to 8-week-old mice were obtained by flushing tibias and femurs with 1 ml ice-cold Flow Cytometry Buffer (FCB, 1x PBS with 2mM EDTA and 0.5+ACU- biotin-free BSA) using 22-gauge needles. Bone marrow hematopoietic c-kit progenitors were enriched by BD Aria II SORP cell sorter using fluorescence-activated cell sorting with anti-CD117-BV421, anti-Sca1-PE, and anti-lineage (Ter119, Gr1, Mac1, B220, Cd3e) antibody cocktail. Total RNA from FACS-sorted hematopoietic cells was isolated using TRIzol Reagent (Invitrogen) following the manufacturer's RNA isolation protocol. For preparation of NGS libraries polyadenylated mRNA from FASC-sorted c-Kit cells enriched from starting material of 100ng total RNA was processed utilizing NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA).
ENA-FIRST-PUBLIC
2023-05-31
ENA-LAST-UPDATE
2023-05-31
External Id
SAMEA112227660
INSDC center alias
Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV); First Faculty of Medicine; Charles University, Czech Republic
INSDC center name
Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV); First Faculty of Medicine; Charles University, Czech Republic
INSDC first public
2023-05-31T00:15:39Z
INSDC last update
2023-05-31T00:15:39Z
INSDC status
public
Submitter Id
E-MTAB-12447:Rps19 R67∆/R67∆ 1
age
8
broker name
ArrayExpress
cell_type
hematopoietic multipotent progenitor cell
common name
house mouse
developmental stage
adult
disease
Diamond-Blackfan anemia
genotype
Rps19 R67∆/R67∆
individual
RPS19_1
isolate
not applicable
organism part
bone marrow
sample name
E-MTAB-12447:Rps19 R67∆/R67∆ 1
scientific_name
Mus musculus
strain
C57BL/6J