Input tuna 5
Homo sapiens
Protocols: AC16 cells were cultured in two 150 mm dishes per point. Ten minutes prior to harvesting, cells were treated with cycloheximide at 100 mg/mL. Cells were washed twice with PBS at room temperature containing 100 mg/mL cycloheximide and harvested at 80% confluence with Trypsin. tunicamycin has been applied to the cells at a concentration of 2 microgramms per milliliter for 4h at 37Â°C Standard extraction with Trizol has been applied according manufacturer recommandations. Library has been constructed according internal Genewiz/azenta pipeline. (genewiz.com)
replicate
4
disease
normal
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
scientific_name
Homo sapiens
common name
human
organism part
heart
developmental stage
adult
genotype
wild type genotype
isolate
not applicable
cell_line
AC16
cell_type
cardiomyocyte
geographic location (country and/or sea)
not collected