Adult CD4+ Naive LINE1 replicate 4
Homo sapiens
Protocols: Naïve CD4+ T cells have been isolated from PBMCs (umbilical for neonatal t cells and peripheral for adult t cells) by density gradient centrifugation with Ficoll-Paque Plus. From PBMCs Naive CD4+ were FACS sorted as CD4+ CD45RA+ CD45RO- Adult naive CD4+ T cells were cultured with LINE1 or scramble (control) ASOs RNA has been extracted with Maxwell RCS simplyRNA Cells kit Libraries were prepared using Illumina Stranded mRNA prep kit and following manufacturer's guidelines
ENA first public
2024-08-30
External Id
SAMEA115120765
INSDC center name
Fondazione Istituto Nazionale di Genetica Molecolare (INGM)
INSDC last update
2024-01-19T16:54:21Z
INSDC status
public
Submitter Id
E-MTAB-13718:Adult CD4+ Naive LINE1 replicate 4
broker name
ArrayExpress
cell_type
naive CD4+ T cell
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
immunophenotype
CD4+ CD45RA+ CD45RO-
individual
4
isolate
not applicable
organism part
blood
sample name
E-MTAB-13718:Adult CD4+ Naive LINE1 replicate 4
scientific_name
Homo sapiens