Sample 5
Rattus norvegicus
Protocols: Hepatic stellate cells were obtained by a two-step enzyme digestion protocol and further purified by magnetic-activated cell sorting (MACS) negative selection (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) to remove possible contamination of CD45+ myeloid cells and CD31+ endothelial cells according to the manufacturer's instructions. Hepatic stellate cells derived sEVs were obtained using an ExoQuick kit (System Biosciences, Inc.). TRIzol (Life Technologies) Illumina TruSeq Small RNA Sample kit (Illumina)
ENA first public
2024-08-30
External Id
SAMEA112773392
INSDC center name
Medical School of Nantong University
INSDC last update
2023-03-15T11:58:01Z
INSDC status
public
Submitter Id
E-MTAB-12764:Sample 5
broker name
ArrayExpress
cell_type
hepatic stellate cell
common name
Norway rat
developmental stage
adult
fraction
extracellular vesicle
genotype
wild type genotype
isolate
not applicable
organism part
liver
phenotype
quiescent
sample name
E-MTAB-12764:Sample 5
scientific_name
Rattus norvegicus
sex
male
strain
Sprague-Dawley
time
0 to 3
weight
450-550