2_WT
Mus musculus
Protocols: 3 biological replicates of 250,000 cells/replicate were processed for each condition. Nuclei were pelleted and frozen after extraction in an isopropyl chamber, and then later thawed on ice to be processed for CUT&RUN in parallel.  CUT&RUN LoV-U for H3K4me was performed as described in (PMID: 36355069), with the following modifications. Antibodies used included anti-H3K4me (antibodies online, ABIN3023251) and anti-rabbit IgG (antibodies online, ABIN101961) at 1:100 dilutions. Library preparation was done using the KAPA Hyper Prep Kit for Illumina sequencing (Cat. #KK8504, KAPA Biosystems) following the manufacturer's guidelines with modifications, as described in (PMID: 36355069).
ENA first public
2024-08-12
INSDC center name
Wallenberg Center for Molecular Medicine (WCMM) Department of Biomedical and Clinical Sciences Division of Molecular Medicine and Virology Linköping University, Sweden
INSDC status
public
Submitter Id
E-MTAB-14338:2_WT
broker name
ArrayExpress
cell_line
mESC
cell_type
mouse embryonic stem cell
collection date
not collected
common name
house mouse
developmental stage
embryo stage
disease
none
genotype
WT
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blastocyst
sample name
E-MTAB-14338:2_WT
scientific_name
Mus musculus
stimulus
None