HCC1806 siRNF40.2
Homo sapiens
Protocols: HCC1806 cells were grown in RPMI GlutaMax medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. siRNA transfections were performed using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer's instructions. RNA was extracted usiung QIAzol® Lysis Reagent (Qiagen) according to the manufacturer's protocol. Libraries were prepared using NEXTflex™ Rapid Illumina Directional RNA-Seq Library Prep Kit (Illumina®) after verifying the RNA integrity on an agarose gel. Library quality was assessed using an Agilent Bioanalyzer 2100.
ENA-FIRST-PUBLIC
2023-07-20T00:18:54Z
ENA-LAST-UPDATE
2023-07-20T00:18:54Z
External Id
SAMEA110177403
INSDC center alias
University Medical Center Gottingen Department of Gynecology and Obstetrics Molecular Gynecology
INSDC center name
University Medical Center Gottingen Department of Gynecology and Obstetrics Molecular Gynecology
INSDC first public
2023-07-20T00:18:54Z
INSDC last update
2023-07-20T00:18:54Z
INSDC status
public
Submitter Id
E-MTAB-11860:HCC1806 siRNF40.2
broker name
ArrayExpress
cell_line
HCC1806
common name
human
disease
triple-negative breast cancer
genotype
wild type genotype
isolate
not applicable
organism part
mammary gland
sample name
E-MTAB-11860:HCC1806 siRNF40.2
scientific_name
Homo sapiens