MY73-OE_rep3
Arabidopsis thaliana
Protocols: Total RNA were extracted from pooled Arabidopsis siliques 12 days after flowering (DAF). Arabidopsis thaliana seedlings are grown chambers under constrant temperature of 23°C and a photoperiod of 16 h light (100‐150 μmol m−2 sec−1 354 illumination)/8 h dark. Total RNA extraction was carried out using the Monarch Total RNA Miniprep Kit (New England Biolabs), following the manufacturer's protocols. Subsequently, DNase I (New England Biolabs) was employed to remove genomic DNA, according to the manufacturer's instructions. RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer's instruction
isolate
not applicable
organism
Arabidopsis thaliana
organism
Arabidopsis thaliana
collection date
not collected
scientific_name
Arabidopsis thaliana
common name
thale cress
organism part
Siliques
age
12 Days After Flowering (DAF)
genotype
MYB73-OE
geographic location (country and/or sea)
not collected