U87 Vector
Homo sapiens
Protocols: Patient brain tumor samples were collected during surgery and glioma stem cells were purified from the samples. Glioma cells were made to grow into gliomaspheres in a growth factor cocktail containing media, prior to centrifugation and nucleic acid extraction. Total RNA was extracted and purified from cellular samples using TRIzol Reagent (Ambion, Cat# 15596018), while mRNA was purified using PolyATract mRNA Isolation Systems Kit (Promega, Cat#Z5300) from total RNA samples. RNA transcripts were fragmented into ~100 nucleotide segments and isolated for m6A enrichment using m6A Dynabeads (Synaptic Systems, Cat# 202003) for each sample. This allowed for input RNA and m6A enriched RNA to be analyzed following MeRIP-Seq using RADAR. Stranded mRNA Library Preparation Kits (Illumina, Cat# 20020594) were used for sequencing library preparations, with unique adapter indices ligated to each sample to allow for multiplexing (TruSeq RNA Single Indices Set A, Illumina, Cat# 20020492).
ENA first public
2024-02-17
INSDC center name
UCLA
INSDC status
public
Submitter Id
E-MTAB-13765:U87 Vector
broker name
ArrayExpress
cell_type
gliomasphere
collection date
not collected
common name
human
disease
glioma
genotype
IDH-WT
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
brain
progenitor cell type
glioma stem cell
sample name
E-MTAB-13765:U87 Vector
sampling site
tumor
scientific_name
Homo sapiens