Chromium_DLBCL_breast
Homo sapiens
Protocols: Curls with 2x25 micrometers for lung, breast and DLBCL were sectioned from FFPE blocks. Nuclei were extracted using snPATHO protocol9, based on the protocol from 10X (CG000632). The nuclei were counted on the LUNA-FX7 cell counter (AO/PI viability kit, Logos). Pools of four samples were processed together as shown in Supplementary Table 4, targeting 40,000 nuclei recovery per pool (10,000 nuclei per sample). Replicas for two donors B3 and B4 were processed independently. The protocol was followed by the manufacturer's protocol Chromium Fixed RNA profiling - Multiplexed samples (CG000527).
isolate
not applicable
disease
diffuse large B-cell lymphoma; breast cancer
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
scientific_name
Homo sapiens
common name
human
geographic location (country and/or sea)
not collected