Jur_143_1_t
Homo sapiens
Protocols: For each immunoprecipitation reaction 30 million transduced cells were used. The Ago2-RIP was performed using Magna RIP Kit (Merck) and anti-Ago2 antibody (Abnova, H00027161-M01). For total RNA isolation 3 million cultured Jurkat T-ALL cells were harvested and rinsed twice with phosphate buffered saline. Total RNA was isolated using miRNeasy Kit (Qiagen). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). For each immunoprecipitation reaction 30 million transduced cells were used. The Ago2-RIP was performed using Magna RIP Kit (Merck) and anti-Ago2 antibody (Abnova, H00027161-M01). For total RNA isolation 3 million cultured Jurkat T-ALL cells were harvested and rinsed twice with phosphate buffered saline.
ENA first public
2024-08-31
External Id
SAMEA114514186
INSDC center name
Institute of Human Genetics Polish Academy of Sciences
INSDC last update
2023-10-18T18:52:09Z
INSDC status
public
Submitter Id
E-MTAB-13462:Jur_143_1_t
broker name
ArrayExpress
cell_line
Jurkat
cell_type
lymphoblast
collection date
not collected
common name
human
developmental stage
juvenile
disease
T-cell acute lymphoblastic leukemia
genotype
pCDH_CMV_MCS_EF1_GFP_puro pre-mir-143
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
bone marrow
replicate
1
sample name
E-MTAB-13462:Jur_143_1_t
scientific_name
Homo sapiens