3DAE_R3
Arabidopsis thaliana
Protocols: After around 35 days of growth all stage 12c flowers on the main stem were emasculated and harvested after 2, 3, or 4 days. Unpollinated pistils of emasculated wild-type flowers were dissected as described previously. For each time point the ovules of over 75 pistils (approximately 4000 ovules) were collected on dry ice with the help of a vacuum-driven micro-aspirator (Sanchez-Leon et al., 2012). The whole time series was sampled in three independent batches (four time points in each round, 2 to 4 DAE). Arabidopsis seeds (Columbia-0, Col-0) were sown on Jiffy-7C propagation plugs (Jiffy) and stratified for two to three days at 4 °C. Plants were grown for about 35 days at 22°C under a 16 h light/8 h dark regime before being emasculated. After subsequent tissue grinding, RNA was extracted with the Spectrum Plant Total RNA kit (Sigma). RNA quantity and quality was assessed by spectrophotometric analysis (NanoDrop ND-2000, Thermo) and on-chip electrophoresis (RNA 6000 Nano Kit, Agilent). Library preparation was done using the TruSeq unstranded RNA Sample Preparation Kit v2 (Illumina).
ENA-FIRST-PUBLIC
2023-05-30
ENA-LAST-UPDATE
2023-05-30
External Id
SAMEA111350653
INSDC center alias
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology, VIB, Gent, Belgium
INSDC center name
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology, VIB, Gent, Belgium
INSDC first public
2023-05-30T11:00:04Z
INSDC last update
2023-05-30T11:00:04Z
INSDC status
public
Submitter Id
E-MTAB-12174:3DAE_R3
broker name
ArrayExpress
common name
thale cress
developmental stage
mature non-fertilized ovule
ecotype
Col-0
genotype
wild type genotype
isolate
not applicable
organism part
plant ovule
sample name
E-MTAB-12174:3DAE_R3
scientific_name
Arabidopsis thaliana