75% WHC rep 1
soil metagenome
Surface soils (0-20 cm deep, 5 cm diameter cores) were collected from 16 randomly selected locations immediately adjacent to the Tall Wheatgrass Irrigation Field Trial experiment in Prosser, WA. Samples were transported to the Pacific Northwest National Laboratory (PNNL) on ice and stored at 4℃ until processing. Soils were homogenized on ethanol-sterilized 4 mm sieves, and rocks were removed. Sixty grams (dry weight soil equivalent) were weighed into autoclaved 4 oz jars, and the soils were brought to 75% water holding capacity by addition of sterile deionized water. Three replicate incubations were set up for each of the two sampling time points (n=6). First, the soils were preincubated for one week during which time they were gradually exposed to increasing temperature until the target incubation temperature of 30C average summer temperature  was reached. The incubation temperatures were: 10 C on day 1, 15C on day 3, 25C on day 5 and 30C on day 7. During the pre-incubation water was added to the soils daily to maintain them at 75% water holding capacity by mass. Once at 30C, three samples were harvested representing the pre-desiccation soils. Sample was stored at -80C until being processed for DNA and RNA extractions.
collection_date
2020-06
depth
0-20cm
elev
250m
env_broad_scale
ENVO:01000177 (grassland biome)
env_local_scale
ENVO:00005750 (grassland soil)
env_medium
ENVO:00001998 (soil)
geo_loc_name
USA: Washington, Prosser, Irrigated Agricultural Research and Extension Center (IAREC)
lat_lon
46.25 N 119.73 W
replicate
1
sieving
4mm
soil_type
aridisol
water_content
75% WHC
water_content_soil_meth
gravimetric
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil