Sample 4
Mus musculus
Protocols: The trigeminal ganglia were dissected after euthanasia and cardiac perfusion with PBS to remove contaminating blood cells, collected in ice-cold TRI Reagent, and stored at -80°C until processing. both trigeminal ganglia from one mouse were homogenized in 1 ml of TRI Reagent, and then 0.2 ml of isopropanol was added. After centrifugation, RNA was purified from the aqueous phase using the Direct-zol RNA MiniPrep kit (Cat #R2052, Zymo Research, Irvine, CA, USA) following the manufacturer's instructions. The concentration and purity of RNA were assessed with a NanoDrop 1000 spectrophotometer (ThermoFisher  Scientific, Waltham,  MA,  USA). RNA-Seq was performed by NovoGene (Sacramento, CA, USA) using the Illumina NovaSeq platform to generate 150 bp paired-end reads.
strain
C57BL/6
disease
dry eye syndrome
individual
C4
organism
Mus musculus
organism
Mus musculus
collection date
not collected
sex
female
scientific_name
Mus musculus
common name
house mouse
organism part
trigeminal ganglion
developmental stage
adult
genotype
wild type genotype
isolate
not applicable
age
8
geographic location (country and/or sea)
not collected