N0_1
Homo sapiens
Protocols: HDLEC were harvested from culture at 70-80% confluency by trysinization no particular treatment has been applied to the HDLEC cells RNA extraction was performed with a Biobasic EZ-10 Total RNA extraction kit (BS82322) according to manufacturer protocol RNA-seq paired-end libraries have been prepared according to Illumina's protocol using the TruSeq Stranded Total RNA Gold library prep Kit (Illumina, San Diego, USA). Libraries quality was assessed using the HS NGS kit on the Fragment Analyzer (Agilent Technologies, Santa Clara, USA). Libraries were quantified by qPCR using the KAPA Library Quantification Kit (Roche, Basel, Switzerland) to obtain an accurate quantification.
replicate
1
disease
normal
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
scientific_name
Homo sapiens
common name
human
organism part
skin of prepuce of penis
developmental stage
neonate
genotype
wild type genotype
isolate
not applicable
cell_line
primary cell line
cell_type
Human Dermal Endothelial Cell
geographic location (country and/or sea)
not collected