insect metagenome
organism
insect metagenome
broad-scale environmental context
cropland biome
collection date
2020
target gene
COI
geographic location (longitude)
10.44338024
environmental medium
nesting material
geographic location (latitude)
52.28390526
scientific_name
insect metagenome
pcr primers
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC; REV: GCGAGCTGCGCTAtgcGTRATWGCHCCDGCTARWACWGG
local environmental context
nest tube of cavity-nesting hymenoptera
sample material processing
feces: collected from nest tube, stored at -20�C, swabs: collected using DNA-free swab in PBS, stored at -20�C
project name
eDNA cavity-nesting wild bees
sequencing method
Illumina MiSeq
nucleic acid extraction
ZymoBIOMICS DNA Microprep Kit
nucleic acid amplification
PCR
multiplex identifiers
FWD: ACACCAT; REV: GGGTAAT
geographic location (country and/or sea)
Germany
ENA-LAST-UPDATE
2022-11-01