activated sludge metagenome
ENA first public
2025-01-16
External Id
SAMEA112329382
INSDC center name
NETHERLANDS INSTITUTE OF ECOLOGY
INSDC last update
2023-01-16T13:45:18Z
INSDC status
public
Submitter Id
SBR1-AGS-day67-16S
amount or size of sample collected
0.015
broad-scale environmental context
Sequencing batch reactor
collection date
2021-01-29
environmental medium
Synthetic wastewater
geographic location (country and/or sea)
Netherlands
geographic location (latitude)
51.98699968
geographic location (longitude)
5.67116169
geographic location (region and locality)
Gelderland
local environmental context
Granulated biomass, aerobic granular sludge
nucleic acid amplification
PCR
nucleic acid extraction
Qiagen DNAeasy Power Soil Pro
number of replicons
3
oxygenation status of sample
aerobic
pH
7.25
pcr primers
616*F-1132R
project name
Local Treatment of Urban sewage and Streams for Healthy Reuse (LOTUSHR) - www.lotushr.org
reactor type
Sequencing batch reactor
sample material processing
15mL of harvested photogranules were homogenised by a glass/Teflon tissue grinder, aliquoted into five 2 mL Eppendor tubes, centrifuged at 14.870 rcf for 10 min and the supernatant discarded, the cell pellets were immediately frozen at -80 �C until further processing.
sample name
SBR1-AGS-day67-16S
sample volume or weight for DNA extraction
0.2
scientific_name
activated sludge metagenome
sequencing method
Illumnia MiSeq
sludge retention time
14
target gene
16S rRNA gene
wastewater type
Synthetic wastewater