Bacteroides uniformis
ENA first public
2024-09-21
INSDC center name
EMBL Heidelberg
INSDC status
public
Submitter Id
spire_mag_01778242
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
megahit 1.2.9
binning parameters
default
binning software
metabat 2.12.1
broad-scale environmental context
human digestive system
broker name
EBI-EMG
collection date
missing: third party data
completeness score
51.0
completeness software
checkm2 0.1.3
contamination score
3.74
environmental medium
stool
geographic location (country and/or sea)
USA
geographic location (latitude)
missing: third party data
geographic location (longitude)
missing: third party data
investigation type
metagenome-assembled genome
isolation_source
human gut metagenome
local environmental context
human gut
metagenomic source
human gut metagenome
project name
Neonates at heightened risk of childhood atopy and asthma are characterized by inter-kingdom perturbation of their gut microbiota and metabolic dysfunction that includes fecal enrichment of the linoleic acid metabolite, 12,13 DiHOME1. Treatment of human dendritic cells (DCs) with this oxylipin decreased IL-10, altered expression of peroxisome-proliferator-activated-receptor-γ (PPARγ)-regulated genes, and decreased regulatory T cell (Tregs) frequency ex vivo. Mice treated with 12,13 DiHOME and subjected to a model of airway allergic sensitization had exacerbated pulmonary inflammation and decreased lung Tregs. Fecal metagenomic sequencing from neonates who developed atopy and/or asthma revealed a significant increase in bacterial epoxide hydrolase (EH) genes. Three of these bacterial EHs can produce 12,13 DiHOME in vitro. The abundance of fecal oxylipins and bacterial EH genes in neonatal stool can be combined with known early-life risk factors to predict neonatal risk of atopy at age two and asthma at age four.
sample derived from
SAMEA1069170
sample name
spire_mag_01778242
scientific_name
Bacteroides uniformis
sequencing method
Illumina HiSeq 4000
taxonomic identity marker
multi-marker approach