Pseudomonas phage UNO-G1W1
isolate
Extracted by auger drill ice sampling from Lake Wanahoo near Wahoo, Nebraska. The thawed sample was centrifuged at 6000 rpm (SS-34 rotor) for 10 min at 4C to pellet debris and the supernatant was removed and syringe filtered (0.22 m). The filtered sample was diluted and log phase host bacteria (P. fluorescens Migula strain; ATCC 27663TM) was added and incubated at room temperature for 20 minutes to allow viral invasion. These incubations were added to 3.5 mL of molten 0.7% agarose (45C), then plated and incubated overnight at 26C. A resulting plaque was isolated (UNO-G1W1) and amplified for characterization. A high-titer stock was prepared and used for gDNA isolation with phenol-chloroform extraction for sequencing
lab_host
Pseudomonas fluorescence Migula
collection_date
2016-12-20
geo_loc_name
USA: NE
isolation_source
Lake Wanahoo, ice cover
collected_by
University of Nebraska at Omaha
lat_lon
41.251597 N 96.612549 W
BioSampleModel
Viral