sponge metagenome
collection_date
2022-08-09
env_broad_scale
Marine benthic biome [ENVO_01000024]
env_local_scale
shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]
env_medium
Sea water [ENVO_00002149]
geo_loc_name
Russia: Dalniye Zelentsy, Guba Yarnyshnaya, Barents Sea
host
Halichondria panicea
lat_lon
69.10177 N 36.06234 E
depth
0 m
host_common_name
Breadcrumb sponge
host_life_stage
Adult attached sponge
host_subject_id
3383
samp_collect_device
Manual picking
samp_mat_process
A fragment of sponge tissue was diced and fragmented by razor and forceps in 25 mL of sterile marine water. The obtained suspension was centrifuged at 1000 rpm for 3 min. The supernatant was collected and centrifuged further at 4700 rpm for 10 min. Resulatnt pellet was used for DNA extraction. A V3-V4 region of 16S rRNA gene was amplified using standard degenerate primers fused with sequencing adapters (Illumina). PCR Forward Primer = CCTACGGGNGGCWGCAG, PCR Reverse Primer = GACTACHVGGGTATCTAATCC, adapter sequences are not shown.
samp_size
A fragment of an individual sponge, ~50 g
samp_vol_we_dna_ext
1 cm3
temp
12.5 C
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.host-associated