soil metagenome
sample volume or weight for DNA extraction
0.0731
broad-scale environmental context
sub-arctic boreal forest
collection date
2015-08-15
target gene
ITS
geographic location (longitude)
27.98455
sample collection device
soil corer 3 cm diameter
sample material processing
humus and mineral soil samples separated in the field, stored at outdoor temperature during sampling, stored at -20 degrees C after sampling
storage conditions (fresh/frozen/other)
frozen -20 degrees C
pH
4,2
geographic location (country and/or sea)
Norway
elevation
117
mean annual and seasonal precipitation
annual 450 mm
organism
soil metagenome
current land use
winter reindeer grazing
environmental medium
soil
geographic location (latitude)
70.03243
scientific_name
soil metagenome
target subfragment
ITS2
geographic location (region and locality)
Polmak
pcr primers
forward primer:GTGARTCATCGAATCTTTG;reverse primer:TCCTCCGCTTATTGATATGC
mean annual and seasonal temperature
seasonal 12C and -12C in the warmest (July) and coldest (January) months respectively
pcr conditions
initial denaturation:98degC_1min: (denaturation:98degC_10sec; annealing:57degC_20sec; elongation:72degC_30sec)cycles=27; final elongation:72degC_7min
local environmental context
mountain birch forest
depth
0-0.03
current vegetation
mountain birch with an understory layer dominated by dwarf shrubs (Empetrum nigrum ssp. hermaphroditum, Vaccinium myrtillus, V. vitis-idaea)
project name
Polmak soil 2015
sequencing method
Ion Torrent PGM
history/extreme events
severe moth outbreaks 2002-2008
composite design/sieving (if any)
five soil cores pooled together
soil horizon
A horizon