Bacteroides uniformis
ENA first public
2024-09-21
INSDC center name
EMBL Heidelberg
INSDC status
public
Submitter Id
spire_mag_00735194
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
megahit 1.2.9
binning parameters
default
binning software
metabat 2.12.1
broad-scale environmental context
human digestive system
broker name
EBI-EMG
collection date
2015-12-15
completeness score
83.77
completeness software
checkm2 0.1.3
contamination score
5.68
environmental medium
stool
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.91670000
geographic location (longitude)
7.46670000
investigation type
metagenome-assembled genome
isolation_source
human gut metagenome
local environmental context
human gut
metagenomic source
human gut metagenome
project name
Purpose: Retinal artery occlusion usually occurs on a background of atherosclerotic disease and patients are at risk for cerebral stroke and ischemic heart disease. Atherosclerosis has been associated with alterations in the intestinal microbiome. Methods: Twenty nine patients with clinically confirmed non-arteritic retinal artery occlusion and 30 age- and sex-matched controls were included. The gut metagenome was sequenced using metagenomic shotgun sequencing following standard pipelines of the Illumina HiSeq 3000 platform. The taxonomic composition of the gut microbiomes was described and the functional profiles of the identified microbes were identified. Results: On the phylum level, the relative abundance of Bacteriodetes was decreased in patients with retinal artery occlusion (39.2 ± 16.7%) compared to controls (46.7 ± 17.6%) whereas the opposite applied for the phylum of Firmicutes (39.6 ± 20.0% in RAO and 40.8 ± 16.0% (s.d.) in controls). Several genera and species such as Actinobacter, Bacteroides stercoris, Faecalibacterium prausnitzii were relatively enriched in patients with retinal artery occlusion, whereas others such as Odoribacter or Lachnospiraceae were significantly lower. Patient's gut microbiomes were enriched in genes of the cholesterol metabolism pathway. Conclusions: Alterations in the gut microbiome are associated with retinal artery occlusion, suggesting that retinal artery occlusion could be targeted by microbiome-altering interventions.
sample derived from
SAMEA104500037
sample name
spire_mag_00735194
scientific_name
Bacteroides uniformis
sequencing method
Illumina HiSeq 3000
taxonomic identity marker
multi-marker approach