Bacteroides uniformis
ENA first public
2024-09-21
INSDC center name
EMBL Heidelberg
INSDC status
public
Submitter Id
spire_mag_02379151
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
megahit 1.2.9
binning parameters
default
binning software
metabat 2.12.1
broad-scale environmental context
human digestive system
broker name
EBI-EMG
collection date
not collected
completeness score
94.43
completeness software
checkm2 0.1.3
contamination score
0.12
environmental medium
stool
geographic location (country and/or sea)
not provided
geographic location (latitude)
missing: third party data
geographic location (longitude)
missing: third party data
investigation type
metagenome-assembled genome
isolation_source
human gut metagenome
local environmental context
human gut
metagenomic source
human gut metagenome
project name
Centenarians, who are living more than a century, represent the ultimate model of successful longevity associated with less susceptibility to aging-associated illness and chronic inflammation. The gut microbiota has been considered as a criticial determinant for human health and longevity. Here we show that centenarians (average 107 yo) have distinct gut microbiome enriched in microbes capable of generating unique secondary bile acids, include iso-,3-oxo, and iso-allo-lithocolic acid (LCA), as compared to elderly (85-89 yo) and young (21-55 yo) controls. In particular, iso-allo-LCA biosysnthesis pathway had not been defined. By screening of 68 bacterial isolates from a centenarian's faecal microbiota, we identified Paraceteroides merdae and Odoribacteraceae stains as effective producers of iso-all-LCA. Furthermore, we revealed that 5'-reductase (5AR) and 3'-hydroxysteroid dehydrogenase (3'HSDH) were responsible for the iso-allo-LCA generation by generating and testing mutant P. merdae strains. Iso-allo-LCA exerted the most potent antimicrobial effects among the test bile acid compounds, against gram-positive (but not gram-negative) multidrug-resistant pathogens, such as Clostridium difficile and vancomycin-resitant Enterococcus faecium. These findings suggest that specific bile acid metabolism may be involved in reducing the risk of acquiring pathobionts and their related diseases, thereby potentially contributing to longevity.
sample derived from
SAMN20081838
sample name
spire_mag_02379151
scientific_name
Bacteroides uniformis
sequencing method
Illumina NovaSeq 6000
taxonomic identity marker
multi-marker approach