biofilm metagenome
ENA first public
2025-01-16
External Id
SAMEA112329177
INSDC center name
NETHERLANDS INSTITUTE OF ECOLOGY
INSDC last update
2023-01-16T12:43:30Z
INSDC status
public
Submitter Id
PSBR_Day105_18S
broad-scale environmental context
Photobioreactor
collection date
2019-12-19
environmental medium
Water
geographic location (country and/or sea)
Netherlands
geographic location (latitude)
51.98699968
geographic location (longitude)
5.67116169
geographic location (region and locality)
The Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, Gelderland
local environmental context
Granulated biomass, photogranule
nucleic acid amplification
PCR
nucleic acid extraction
Qiagen DNAeasy Power Soil Pro
number of replicons
3
oxygenation status of sample
aerobic
pH
6.8
pcr primers
616*F-1132R
project name
Local Treatment of Urban sewage and Streams for Healthy Reuse (LOTUSHR) - www.lotushr.org
reactor type
Photobioreactor operated in sequencing batch mode
sample material processing
15mL of harvested photogranules were homogenised by a glass/Teflon tissue grinder, aliquoted into five 2 mL Eppendor tubes, centrifuged at 14.870 rcf for 10 min and the supernatant discarded, the cell pellets were immediately frozen at -80 �C until further processing.
sample name
PSBR_Day105_18S
sample volume or weight for DNA extraction
0.2
scientific_name
biofilm metagenome
sequencing method
Illumnia MiSeq
sludge retention time
7
target gene
18S rRNA gene
wastewater type
Synthetic wastewater