WSA_LngSP9258462_imaged
Homo sapiens
Protocols: Samples no larger than 0.5cm2 were cut from each of the 5 locations of lung (trachea, bronchi at the 2nd/3rd generation, bronchi at the 4th generation, upper left lobe parenchyma and lower left lobe parenchyma) and additional small airway. Majority of the parenchyma was removed from the airway tissues, embedded in OCT and flash frozen in dry-ice cooled isopentane at -55°C.  Haematoxylin and eosin staining was used to determine the morphology of tissue blocks, in order to choose those to proceed to Visium. Ten micron sections were then cut from the blocks onto Visium slides (10X Genomics) and processed according to the manufacturer's protocol. Nucleic acid was extracted according to the standard protocol of the Spatial 3' v1 protocol Tissue sections were loaded onto Visium chips according to the standard protocol of the Spatial 3' v1 protocol. Dual-indexed libraries were prepared, pooled at 2.25nM and sequenced.
disease
normal
individual
A50
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
female
scientific_name
Homo sapiens
common name
human
organism part
lower lobe of lung
developmental stage
adult
isolate
not applicable
age
78
geographic location (country and/or sea)
not collected