Leaf; treated with white light; replicate A
Quillaja brasiliensis
We collected seeds of Quillaja brasiliensis (A.St.-Hil. & Tul.) Mart. (Quillajaceae) from the city of Cangucu (Rio Grande do Sul, Brazil) in March/2018. After in vitro germination, we transferred them to the grow room at 25 C under a 16h/day photoperiod. We detached leaves from 2.8-year-old individuals for the experiments hereby presented. After leaf cleanup, we detached approximately 15 leaves and exposed them to ultraviolet radiation (UV-C germicide lamp, wavelength maximum 254 nm) or white light (control). After incubation, we froze the plant material in liquid nitrogen and stored it at -80 C until the next processing step. We extracted RNA samples from different tissues and growth conditions using the cetyltrimethylammonium bromide method (CTAB) and performed their purification using the RNeasy MinElute Cleanup Kit (QIAGEN, Hilden, Germany). We quality-controlled the samples using fluorometric and spectrophotometric methods before submitting them to sequencing by Novogene (Beijing, People's Republic of China). Libraries have been prepared using the mRNA stranded protocol (Illumina, San Diego, US).
ENA-FIRST-PUBLIC
2023-02-02
ENA-LAST-UPDATE
2023-02-02
External Id
SAMEA112328554
INSDC center alias
IB-RC UNESP
INSDC center name
ib-rc unesp
INSDC first public
2023-02-02T08:19:05Z
INSDC last update
2023-02-02T08:19:05Z
INSDC status
public
Submitter Id
leaf_wlight_A
replicate
A
sample name
leaf_wlight_A
scientific_name
Quillaja brasiliensis
tissue_type
Leaf
treatment
White light