B_MP
Mus musculus
Protocols: To sort astrocytes, mouse neocortices were rapidly harvested then dissociated with Adult Brain Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator using program 37C_ABDK_01. Dissociated samples were then treated with debris and red blood cell removal steps to obtain cell suspensions. For FACS, cells were resuspended in a final volume of 100 uL 0.1% PB buffer. Then cells were incubated with fluorescent conjugated antibodies at 4° C for 30 minutes. The following antibodies were used: ACSA2 APC (1:200, Miltenyi Biotec, 130-116-245) and O4 PE (1:100, Miltenyi Biotec, 30-117-357). ACSA2 APC (+) and O4-PE (-) live single cells were selected as the astrocyte population.   Library preparation was conducted with the Chromium Single Cell 3′ v3.1 Reagent Kit (10x Genomics), following the manufacturer's instructions. Library preparation was conducted with the Chromium Single Cell 3′ v3.1 Reagent Kit (10x Genomics), following the manufacturer's instructions.
ENA first public
2024-10-15
INSDC center name
Centre for Discovery Brain Sciences, University of Edinburgh
INSDC status
public
Submitter Id
E-MTAB-14233:B_MP
age
10 to 12
broker name
ArrayExpress
cell_type
astrocyte
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
HET_APP-WT_FIRE
geographic location (country and/or sea)
not collected
individual
336, 339, 342
isolate
not applicable
library type
Multiplexing
multiplexing cmos
CMO303, CMO304, CMO305
organism part
cerebral cortex
sample name
E-MTAB-14233:B_MP
scientific_name
Mus musculus
sex
female
strain
CBA