Na-9_PO_M0_VDJ
Homo sapiens
Protocols: For each sample, we always FACS sorted (MA900, Sony) an initial pool of 50.000 total peripheral CD3-CD14-CD56- CD19+ IgD- cells and afterward, to enrich for cells of interest, only sorted CD19+CD38low antibody secreting cells (ASCs), CD19hi IgD+ cells and SARS-CoV-2 Spike/RBD PE/tetramer positive B cells, leading to approximately 55000-60000 total sorted cells per sample. Inferred cell types per sample can be found in the corresponding sample_information_filtered.tsv file. Cells were counted and up to 20 000 cells were loaded in the 10x Chromium Controller to generate single-cell gel-beads in emulsion. After reverse transcription, gel-beads in emulsion were disrupted. Barcoded complementary DNA was isolated and amplified by PCR. The scRNA-seq libraries were generated using Chromium Next GEM Single Cell V(D)J Reagent Kit v.1.1 with Feature Barcoding (10x Genomics) according to the manufacturer's protocol. Gene expression (mRNA), ADT and VDJ BCR libraries were constructed.
ENA-FIRST-PUBLIC
2024-01-25T00:32:11Z
ENA-LAST-UPDATE
2024-01-25T00:32:11Z
External Id
SAMEA112749083
INSDC center name
Institut Necker Enfants Malades (INEM), INSERM U1151/CNRS UMS 8253, Universite Paris Cite, Paris, France.
INSDC first public
2024-01-25T00:32:11Z
INSDC last update
2024-01-25T00:32:11Z
INSDC status
public
Submitter Id
E-MTAB-12651:Na-9_PO_M0_VDJ
age
50
broker name
ArrayExpress
cell_type
B cell
clinical information
mRNA vaccinated
common name
human
developmental stage
adult
disease
COVID-19
individual
Na-9
infect
Severe acute respiratory syndrome coronavirus 2 BA.1 Omicron
isolate
not applicable
organism part
blood
sample name
E-MTAB-12651:Na-9_PO_M0_VDJ
scientific_name
Homo sapiens
sex
male