Metagenome-Assembled Genome: ERR9762682_bin.41_MetaWRAP_v1.3_MAG
uncultured Eubacteriales bacterium
This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9762682 of study ERP137678.
ENA-FIRST-PUBLIC
2023-07-13T20:35:05Z
ENA-LAST-UPDATE
2023-07-13T20:35:05Z
External Id
SAMEA114064186
INSDC center alias
EMG
INSDC center name
EMG
INSDC first public
2023-07-13T20:35:05Z
INSDC last update
2023-07-13T20:35:05Z
INSDC status
public
Submitter Id
ERR9762682_bin.41_MetaWRAP_v1.3_MAG
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaspades v3.15.3
binning parameters
MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
binning software
MetaWRAP v1.3
broad-scale environmental context
Human digestive system
broker name
EMG broker account, EMBL-EBI
collection date
2019
completeness score
98.66
completeness software
CheckM
contamination score
0.0
environmental medium
faeces
geographic location (country and/or sea)
Sweden
geographic location (latitude)
6399670.23
geographic location (longitude)
323762.92
investigation type
metagenome-assembled genome
isolation_source
human feces metagenome
local environmental context
colon
metagenomic source
human feces metagenome
project name
Recent studies have shown that probiotic supplementation has beneficial effects on bone metabolism. In a randomized controlled trial (RCT) we demonstrated that supplementation of Lactobacillus reuteri ATCC PTA 6475 reduced bone loss in older women with low bone mineral density. To investigate the mechanisms underlying the effect of L. reuteri ATCC PTA 6475 on bone metabolism, 20 women with the highest changes (good responders) and the lowest changes (poor responders) in tibia total volumetric BMD after one-year supplementation were selected from our previous RCT. In the current study we characterized the gut microbiome composition and function as well as serum metabolome in good responders and poor responders to the probiotic treatment as a secondary analysis.
sample derived from
SAMEA14418832
sample name
ERR9762682_bin.41_MetaWRAP_v1.3_MAG
scientific_name
uncultured Eubacteriales bacterium
sequencing method
Illumina NovaSeq 6000
taxonomic classification
The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__;g__;s__
taxonomic identity marker
multi-marker approach