Sample 1
Mus musculus
Protocols: Cultured cells were lysed in TRI reagent. RNA was isolated using TRI reagent with additional clean-up using a silica column-based isolation method (ISOLATE II RNA Mini Kit #BIO-52071, Bioline), including DNAse treatment. The RNA integrity of each sample was tested using Agilent 2100 Bioanalyser (RNA Nano (5-500 ng/μL)-Chip service) and samples with RNA Integrity Number (RIN) above 7.5 were deemed suitable for sequencing. 1)mRNA enrichment and purification: rRNA depletion or Oligo dT Selection to enrich the mRNA 2)RNA fragment and reverse transcription 3)End repair, add A and adaptor ligation 4)RCR amplification 5)Single strand separation and cyclization 6)DNA nanoball synthesis 7)Sequencing on BGISEQ platform
ENA first public
2024-03-21
External Id
SAMEA112868549
INSDC center name
NTU
INSDC last update
2023-04-04T14:46:56Z
INSDC status
public
Submitter Id
E-MTAB-12834:Sample 1
broker name
ArrayExpress
cell_type
brown fat cell
common name
house mouse
developmental stage
adult
disease
normal
genotype
wild type genotype
growth condition
cultured in vitro
isolate
not applicable
organism part
brown adipose tissue
sample name
E-MTAB-12834:Sample 1
scientific_name
Mus musculus