Triticum aestivum
The seeds were germinated in the soil and the seedlings were transferred to individual pots after the third leaf had appeared. Well-watered plants (control) were held at 50% of the water holding capacity (WHC), which corresponds to the field capacity of the soil, while the waterlogging treatment exceeded the WHC by keeping excess water about 5 cm above the soil surface for 12 days. A pool of all fully developed leaves was quickly collected and stored in liquid nitrogen for biochemical analysis and RNA sequencing. Thereafter, roots of the same plants were gently shaken to remove soil particles, carefully washed and dried with thin paper towels as quickly as possible. A pool of the entire root system was collected for biochemical analysis and RNA sequencing. All samples were immediately stored at -80��C until further analysis. The sampling protocol was carried out in a completely randomized manner.
biological material altitude
112 m
biological material geographic location
Germany
biological material latitude
51.816
biological material longitude
11.283
biological material ploidy
hexaploid
checklist
BSDC00001
collection date
missing: lab stock
cultivar
Chinese Spring
genus
Triticum
geographic location (country and/or sea)
missing: lab stock
growth facility
greenhouse
material source geographic location
missing: lab stock
plant anatomical entity
root
project
Wheat flooding RNA-Seq
replicate
biol rep 1
sample id
IPK_LIMS:4039017
sample name
R_F_T1
species
aestivum
treatment
flooded