Sample 7
Mus musculus
Protocols: Cultured cells were lysed in TRI reagent. RNA was isolated using TRI reagent with additional clean-up using a silica column-based isolation method (ISOLATE II RNA Mini Kit #BIO-52071, Bioline), including DNAse treatment. The RNA integrity of each sample was tested using Agilent 2100 Bioanalyser (RNA Nano (5-500 ng/μL)-Chip service) and samples with RNA Integrity Number (RIN) above 7.5 were deemed suitable for sequencing. 1)mRNA enrichment and purification: rRNA depletion or Oligo dT Selection to enrich the mRNA 2)RNA fragment and reverse transcription 3)End repair, add A and adaptor ligation 4)RCR amplification 5)Single strand separation and cyclization 6)DNA nanoball synthesis 7)Sequencing on BGISEQ platform
ENA first public
2024-03-21
External Id
SAMEA112868546
INSDC center name
NTU
INSDC last update
2023-04-04T14:46:56Z
INSDC status
public
Submitter Id
E-MTAB-12834:Sample 7
broker name
ArrayExpress
cell_type
brown fat cell
common name
house mouse
developmental stage
adult
disease
normal
genotype
Tlr4 -/-
growth condition
cultured in vitro
isolate
not applicable
organism part
brown adipose tissue
sample name
E-MTAB-12834:Sample 7
scientific_name
Mus musculus