Codling moth
Lobesia botrana
host
Vitis vinifera
isolation_source
ManisaTurkey
collection_date
2017-07-25
geo_loc_name
Turkey
lat_lon
38.61 N 27.43 E
filetype
fastq
filename
DIRENCLI_1.fq.gz
instrument_model
Illumina HiSeq 2000
platform
ILLUMINA
librarylayout
paired
designdescription
The Illumina standard kit was used for the mRNA-Seq sample preparation, according to the manufacturer's protocol. Briefly, 1,5 g of each total RNA sample was used for polyA mRNA selection using streptavidin-coated magnetic beads, followed by thermal mRNA fragmentation. The fragmented mRNA was subjected to cDNA synthesis using reverse transcriptase (SuperScript II) and random primers. The cDNA was further converted into double stranded cDNA and, after an end repair process (Klenow fragment, T4 polynucleotide kinase and T4 polymerase), was finally ligated to Illumina paired end (PE) adaptors. Size selection was performed using a 2% agarose gel, generating cDNA libraries ranging in size from 200250 bp. Finally, the libraries were enriched using 15 cycles of PCR and purified by the QIAquick PCR purification kit (Qiagen). The enriched libraries were diluted with Elution Buffer to a final concentration of 10 nM. Each library was run at a concentration of 14 pM on one Genome Analyzer (GAII) lane using 36 bp sequencing.
breed
missing
isolate
missing
tissue
missing
BioSampleModel
Invertebrate