Sample 1
Homo sapiens
Protocols: Calbindin-deficient HARA 3D5 cells were generated by Cas9-mediated mutation of the CALB1 gene, encoding Calbindin. HARA.LTR7-GFP+ and HARA.LTR7-GFP- cells were generated by introduction of a HERVH LTR7-GFP reporter into parental HARA (Calbindin-sufficient) cells, using the Sleeping Beauty transposon system. Cells transduced and expressing the reporter were distinguished from cells either not transduced or not expressing GFP expression. GFP+ and GFP- cells were enriched by cell sorting and kept as separate sublines. Introduction of the HERVH LTR7-GFP reporter does not directly affect Calbindin expression, but GFP reporter expression is a marker for and correlates with Calbindin expression. All four cell populations, were grown in vitro and were processed as one batch at the same time, with cell viability between 89% and 93%. RNA was isolated and reverse transcribed with the 10× 3'-mRNA Seq (v3) kit (10× Genomics). Library prep was performed using the the 10× 3'-mRNA Seq (v3) kit (10× Genomics).
ENA-FIRST-PUBLIC
2023-03-01
ENA-LAST-UPDATE
2023-03-01
External Id
SAMEA13443927
INSDC center alias
Retroviral Immunology The Francis Crick Institute
INSDC center name
Retroviral Immunology The Francis Crick Institute
INSDC first public
2023-03-01T00:16:33Z
INSDC last update
2023-03-01T00:16:33Z
INSDC status
public
Submitter Id
E-MTAB-11550:Sample 1
age
57
broker name
ArrayExpress
cell_line
HARA
cell_type
epithelial cell
common name
human
developmental stage
adult
disease
squamous cell lung carcinoma
ethnic group
Japanese
genotype
wild type genotype
organism part
lung
phenotype
wild type
sample name
E-MTAB-11550:Sample 1
sampling site
pleural effusion
scientific_name
Homo sapiens
sex
male