Sample 2
Homo sapiens
Protocols: Day55 brain organoids from hES H9 cell line were snap frozen and processed for single nuclei isolation. Single nuclei isolation protocol was adapted from “Frankestein protocol” (Hu et al., 2017; Habib et al., 2016; Habib et al., 2017; Lake et al., 2016) and Denisenko et al., 2020. Tissue homogenate was FACS sorted to enrich for DAPI positive nuclei. Nuclei were further processed according to 10X manufacturing instructions\. Fast shaking (FAST) organoids were generated according to CTRL condition but kept on orbital shaker at 78 rpm. Nucleic acid fragments were isolated and size-selected using Dynabead-based magnetic purification following 10x genomics library preparation protocol according to manufacturer's instructions. Single-nuclear RNA-seq libraries were generated using the 10X Genomics Chromium system (version 3 chemistry).
ENA-FIRST-PUBLIC
2022-09-16
ENA-LAST-UPDATE
2022-09-16
External Id
SAMEA13793755
INSDC center alias
MRC LABORATORY OF MOLECULAR BIOLOGY
INSDC center name
MRC LABORATORY OF MOLECULAR BIOLOGY
INSDC first public
2022-09-16T00:15:14Z
INSDC last update
2022-09-16T00:15:14Z
INSDC status
public
Submitter Id
E-MTAB-11564:Sample 2
broker name
ArrayExpress
cell_line
H9
common name
human
growth condition
brain organoid
organism part
blastocyst
progenitor cell type
embryonic stem cell
protocol
fast shaking
replicate
biological replicate 6
sample name
E-MTAB-11564:Sample 2
sex
female
time
55