BS5-C
insect metagenome
ExtractionBlank; long fragment; Replicate C
ENA-FIRST-PUBLIC
2023-12-31T01:28:04Z
ENA-LAST-UPDATE
2023-12-31T01:28:04Z
External Id
SAMEA111538354
INSDC center name
Thuenen-Institute of Biodiversity
INSDC first public
2023-12-31T01:28:04Z
INSDC last update
2023-12-31T01:28:04Z
INSDC status
public
Submitter Id
F-HEAD-4-R-TAIL-9-C
broad-scale environmental context
cropland biome
collection date
2020
environmental medium
nesting material
geographic location (country and/or sea)
Germany
geographic location (latitude)
52.28390526
geographic location (longitude)
10.44338024
local environmental context
nest tube of cavity-nesting hymenoptera
multiplex identifiers
FWD: CACTTAT; REV: AATCATC
nucleic acid amplification
PCR
nucleic acid extraction
ZymoBIOMICS DNA Microprep Kit
pcr primers
FWD: GCTATGCGCGAGCtgcGGWACWGGWTGAACWGTWTAYCCYCC; REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
project name
eDNA cavity-nesting wild bees
sample material processing
feces: collected from nest tube, stored at -20�C, swabs: collected using DNA-free swab in PBS, stored at -20�C
sample name
F-HEAD-4-R-TAIL-9-C
scientific_name
insect metagenome
sequencing method
Illumina MiSeq
target gene
COI