iSAM AGM -Dox GEX 7
Homo sapiens
Protocols: The cells were sorted by FACS as CD34+ live and singlet. hPSCs were differentiated in a xeno-free composition of SFD medium 14, BSA was substituted with human serum albumin, HSA, (Irvine-Scientific). Day 0 differentiation medium, containing 10 ng/ml BMP4 was added to the colonies prior cutting. Cut colonies were transferred to a bacteriological grade well to form embryoid bodies and cultured for two days. At day 2 media was changed and supplemented with 3 uM CHIR (StemMacs). At day 3, medium was changed and supplemented with 5 ng/ml bFGF and 15 ng/ml VEGF. At day 6 media was changed for final haematopoietic induction in SFD medium supplemented with 5 ng/ml bFGF, 15 ng/ml VEGF, 30 ng/ml IL3, 10 ng/ml IL6, 5 ng/ml IL11, 50 ng/ml SCF, 2 U/ml EPO, 30 ng/ml TPO, 10 ng/ml FLT3L and 25 ng/ml IGF1. From day 6 onward, cytokines were replaced every two days. According to 10x manufacturer protocol According to 10x manufacturer protocol
ENA first public
2024-03-21
External Id
SAMEA112878199
INSDC center name
UOE
INSDC last update
2023-04-05T14:39:07Z
INSDC status
public
Submitter Id
E-MTAB-12748:iSAM AGM -Dox GEX 7
broker name
ArrayExpress
cell_line
SFCi55
cell_type
hematopoietic cell
common name
human
developmental stage
adult
disease
normal
growth condition
embryoid body 10 days after differentiation
isolate
not applicable
organism part
skin
progenitor cell type
induced pluripotent stem cell
sample name
E-MTAB-12748:iSAM AGM -Dox GEX 7
scientific_name
Homo sapiens