input_wt_1
Aedes albopictus
ChIP-seq libraries were prepared with the Kapa Hyper Prep Kit for Illumina sequencing (Kapa Biosystems) according to the manufacturer's protocol with the following modifications. 3 ng DNA was used as input, with NEXTflex adapters (Bioo Scientific) and ten cycles of PCR amplification. Post-amplification cleanup and size selection was performed with dual AMPure XP beads selection (0.6× and 0.8×). Size-selected samples were analyzed for purity with a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent), with an average size between 300 and 500 bp.
strain
U4.4 cells
isolate
wildtype U4.4 cells
breed
not applicable
cultivar
not applicable
ecotype
not applicable
age
not applicable
dev_stage
not applicable
collection_date
2021-12-10
geo_loc_name
Netherlands
sex
not applicable
tissue
not applicable
cell_line
U4.4 cell line
cell_subtype
wildtype
collected_by
ET
phenotype
wildtype
treatment
input
replicate
biological replicate=1
library type
ChIP-seq
BioSampleModel
Model organism or animal