U2AF1
Homo sapiens
Protocols: HeLa cells were collected after 72hr post transfection. HeLa were purchased from the American Type Culture Collection (ATCC). Cells were cultivated in Glutamax Dulbecco's modified Eagle's medium (Life technologies) supplemented with 10% Fetal bovine serum (Life technologies) and antibiotics (penicillin 500 u/ml; streptomycin 0.5 mg/ml, Life Technologies). HeLa cells were forward transfected in 96-well plates with a siRNA library against known factors and regulators using SMARTpools (ON TARGET PLUS, Thermo-Scientific) using an automatized robotic procedure (Sciclone Liquid Handling Workstation, Perkin Elmer). Endogenous mRNAs were purified 72 hours post transfection by using oligo dT-coated 96 well plates (mRNA catcher PLUS, Life Technologies) following the manufacturer's instructions. Ultra low input library preparation. Full-length RNA-seq from single cells using Smart-seq2
ENA-FIRST-PUBLIC
2023-12-30T01:19:11Z
ENA-LAST-UPDATE
2023-12-30T01:19:11Z
External Id
SAMEA11322783
INSDC center name
Centre for Genomic Regulation (CRG)
INSDC first public
2023-12-30T01:19:11Z
INSDC last update
2023-12-30T01:19:11Z
INSDC status
public
Submitter Id
E-MTAB-11202:U2AF1
broker name
ArrayExpress
cell_line
HeLa
common name
human
developmental stage
adult
disease
cervical carcinoma
isolate
not applicable
organism part
cervix epithelium
sample name
E-MTAB-11202:U2AF1
scientific_name
Homo sapiens