metagenome
pond water samples were collected from four open-air microalgae ponds in Mesa, Arizona, USA
isolation_source
microalgae pond growing Scenedesmus obliquus UTEX 393 - pond 2
collection_date
2025-02
geo_loc_name
USA: Arizona, Mesa
lat_lon
33.30495890 N 111.67303543 W
samp_mat_process
Resuspended and homogenized pond water (200 ml) was vacuum-filtered through a 3.0 µm mixed cellulose ester (MCE) filter, which was then placed in a 5 ml tube with lysis beads. The flowthrough was re-filtered through a 0.45 µm MCE filter and similarly processed. The final flowthrough was concentrated using an Innovaprep CP Select with an Ultra CPT and the default Ultra protocol, then eluted in 1 ml of buffer The eluted sample was combined with the 0.45 µm filter in the lysis tube. Nucleic acids were isolated using the Qiagen PowerWater DNeasy kit per the manufacturer's protocol.
samp_size
200ml
BioSampleModel
Metagenome or environmental