Male Pupae Genomic DNA sample from Bradysia coprophila for Phase Genomics Hi-C library (2018)
Bradysia coprophila
In 2018, male pupae (Bradysia coprophila) were collected in the laboratory of Susan Gerbi (Brown University), flash frozen in bulk, and stored at -80*C. Briefly, for each batch, late larvae were picked from vials and placed on agar petri plates (food and debris were removed from larvae) where they entered pupation asynchronously over 2-4 days . The mixed-stage pupae were collected in 2X SSC in an Eppendorf tube, centrifuged to remove the buffer, washed once with 1 ml 2X SSC and again centrifuged as much of the buffer removed as possible. The vial was then flash frozen in liquid nitrogen and stored at -80*C. Approximately 1000 pupae were collected across 11 tubes this way. Frozen pupae were shipped to Phase Genomics (Seattle, WA) on dry ice where they used the material as needed to prepare a Phase Genomics Proximo Hi-C sequencing library.
breed
Holo2
isolation_source
Lab Strain
collection_date
2018
geo_loc_name
USA: Northeast
tissue
whole pupae
age
mixed pupae
collected_by
Jacob E. Bliss, Susan A. Gerbi, John M. Urban; Brown University
sex
male
Hi-C library prep performed by
Phase Genomics (Seattle, WA)
Sequence data generated by
Phase Genomics (Seattle, WA)
BioSampleModel
Invertebrate