Cell culture; log phase; replicate C
Quillaja brasiliensis
We collected seeds of Quillaja brasiliensis (A.St.-Hil. & Tul.) Mart. (Quillajaceae) from the city of Cangucu (Rio Grande do Sul, Brazil) in March/2018. After in vitro germination, we explanted the seedlings and submitted them to callogenesis. We obtained calluses from in vitro germinated explants and submitted them to cellular suspension induction. We maintained the cell cultures in MS medium supplemented with naphthaleneacetic acid (5 mg/L) and kinetin (0.1 mg/L) in the absence of light, using 250 mL culture flasks under the agitation of 120 RPM. After establishing the growth profile, we collected three flasks for each growth phase of the liquid cell cultures: lag phase (three days), log phase (seven days), and stationary phase (21 days). For the sample collection, cells were filtered using the Buchner funnel, washed with distilled water, and flash-frozen in liquid nitrogen. We stored the collected material at -80 C until the next processing step. We extracted RNA samples from different tissues and growth conditions using the cetyltrimethylammonium bromide method (CTAB) and performed their purification using the RNeasy MinElute Cleanup Kit (QIAGEN, Hilden, Germany). We quality-controlled the samples using fluorometric and spectrophotometric methods before submitting them to sequencing by Novogene (Beijing, People's Republic of China). Libraries have been prepared using the mRNA stranded protocol (Illumina, San Diego, US).
ENA-FIRST-PUBLIC
2023-02-02
ENA-LAST-UPDATE
2023-02-02
External Id
SAMEA112328562
INSDC center alias
IB-RC UNESP
INSDC center name
ib-rc unesp
INSDC first public
2023-02-02T12:22:17Z
INSDC last update
2023-02-02T12:22:17Z
INSDC status
public
Submitter Id
cc_log_C
growth_phase
log
replicate
C
sample name
cc_log_C
scientific_name
Quillaja brasiliensis
tissue_type
Cell culture