S. pyogenes SF370 transformed with pSpy0C2, clone 1
Streptococcus pyogenes M1 GAS
S. pyogenes SF370 was transformed with Alw44I-linearized plasmid pSpy0C2, and transformants were selected on TSA plates with 5% sheep blood and 20 ï¿½g/mL chloramphenicol. A single colony was grown in THY without antibiotic and flash-frozen in liquid nitrogen in THY with 20% glycerol. The strain was then streaked on a TSA plate with 5% sheep blood, grown overnight in THY and finally grown to early stationary growth phase in fresh THY medium. Cells were pelleted, washed with PBS and stored at -80 degree C.
strain
SF370
organism
Streptococcus pyogenes M1 GAS
collection date
2023-10-11
serotype (required for a seropositive sample)
M1
scientific_name
Streptococcus pyogenes M1 GAS
collected_by
Max Planck Unit for the Science of Pathogens
antibiotic_resistance
CmR
culture_medium
THY
genotype
amyA::pSpy0C2
geographic location (country and/or sea)
Germany