plant metagenome
bacterial endophytes from cabbage tissues
library_ID
S97
title
MiSeq of vegetable endophytic bacterial communities: vegetable farms
library_strategy
AMPLICON
library_source
METAGENOMIC
library_selection
PCR
library_layout
paired
platform
ILLUMINA
instrument_model
Illumina MiSeq
design_description
The hypervariable V4 region of 16S rRNA library was constructed by following Illumina MiSeq protocol. Briefly, the protocol included a first stage “amplicon PCR” step involving 0.2 µM concentrations of primers 341F (forward) and 805R (reverse). Sequences were demultiplexed and depleted of Illumina barcodes and primers by using MiSeq reporter and Trimmomatic software
filetype
fastq
filename
S97_1.fq.gz
filename2
S97_2.fq.gz
host
vegetable
isolation_source
cabbage_root
collection_date
2019-10/2021-04
geo_loc_name
South Africa: North West
lat_lon
26.11 S 28.3 E
ref_biomaterial
not applicable
rel_to_oxygen
not applicable
samp_collect_device
not applicable
samp_mat_process
not applicable
samp_size
not applicable
source_material_id
not applicable
BioSampleModel
Metagenome or environmental