Aedes aegypti female midgut blood fed 24h replicate 2
Aedes aegypti
Female Aedes aegypti mosquitoes were artifficially fed on commercial human blood (BF). Intact midguts (15 per bioological replicate) were collected at 24h after blood feeding and placed directly into Trizol reagent. RNA extraction was performed according to manufacturer`s specifications and analyzed with an Agilent 2100 Bioanalyzer (Agilent, USA). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.
replicate
Aedes aegypti female midgut blood fed 24h replicate 2
isolate
Aedes aegypti red eye
breed
missing
host
not applicable
isolation_source
not applicable
collection_date
2022-07
geo_loc_name
USA
tissue
midgut
BioSampleModel
Invertebrate