H3K9me3_ChIP_piwi6KO#10_1
Aedes albopictus
ChIP-seq libraries were prepared with the Kapa Hyper Prep Kit for Illumina sequencing (Kapa Biosystems) according to the manufacturer's protocol with the following modifications. 3 ng DNA was used as input, with NEXTflex adapters (Bioo Scientific) and ten cycles of PCR amplification. Post-amplification cleanup and size selection was performed with dual AMPure XP beads selection (0.6× and 0.8×). Size-selected samples were analyzed for purity with a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent), with an average size between 300 and 500 bp.
strain
U4.4 cells
isolate
Piwi6 KO#10 U4.4 cells
breed
not applicable
cultivar
not applicable
ecotype
not applicable
age
not applicable
dev_stage
not applicable
collection_date
2021-12-10
geo_loc_name
Netherlands
sex
not applicable
tissue
not applicable
cell_line
U4.4 cell line
cell_subtype
Piwi6 Knockout clone#10
collected_by
ET
phenotype
PIWI6 Knockout clone#10
treatment
H3K9me3-ChIP
replicate
biological replicate=1
library type
ChIP-seq
BioSampleModel
Model organism or animal