CRISPR/dCas13(Rx) Derived RNA N6-methyladenosine (m6A) Dynamic Modification in Plant: RNA-seq
Gossypium hirsutum
N6-methyladenosine (m6A) is the most prevalent internal modification of mRNA. It plays an important role in regulating plant growth, development, and stress resistance. There is still a lack of effective tools to precisely modify m6A sites of individual transcripts in plants. Here, we have developed programmable m6A editing tools by combining CRISPR/dCas13(Rx) with the methyltransferase GhMTA (Targeted RNA Methylation Editor, TME) or the demethyltransferase GhALKBH10 (Targeted RNA Demethylation Editor, TDE).