trim49_2
Homo sapiens
Protocols: Cells were collected 7 days after sgRNAs infection Cells were cultured in RPMI medium supplemented with 10% FBS and 1% P/S. Cells were subsequently infected with the LentiCas9-Blast and pLKO5.sgRNA.EFS.GFP containing the specific sgRNAs (TRIM28_sgRNA_49, TRIM28_sgRNA_50, NT2 (non-targeting sgRNA)) Total RNA was extracted by Maxwell RSC simplyRNA Cells kit(Promega, Madison, Wisconsin, USA) Libraries were obtained starting from 500ng of total RNA following the Illumina TruSeq Stranded Total RNA preparation protocol.