SHPC98
Mus musculus
Protocols: Molar tooth germs were bilaterally dissected from the mandible at ED 13.5. Tooth primordium was split into anterior and posterior part and posterior part of tooth germs was cultured for 0, 16 or 24 hours respectively on PET track–etched membrane. The samples were harvested in the RNA latter and stored in -20°C. RNA was isolated by standard protocol with Quiagen RNAeasy micro kit and Precellys®24 homogenizator and stored at -80°C. RNA-Seq libraries were generated according to manufacturer's instructions from 75 ng of total RNA using the Illumina Stranded mRNA Prep, Ligation kit and IDT for Illumina RNA UD Indexes Ligation (Illumina, San Diego, USA)