Sample 11
Staphylococcus aureus
Protocols: Overnight culture in Tryptic Soy Broth (TSB) with Chloramphenicol (for plasmid selection) at 37C with 220rpm shaking was diluted 1:20 in fresh medium and incubated for 1.5h. Bacteria were washed twice in RNAlater and RNA was isolated using the RNeasy Mini kit (Qiagen) following manufacturerâ€™s protocol including DNAse treatment (Turbo DNase kit, Invitrogen).  Paired end 150 bp RNAseq was performed without rRNA depletion by the Functional Genomics Center Zurich (FGCZ) of University of Zurich and ETH Zurich using the TruSeq RNA Library Prep Kit on an Illumina NovaSeq X Plus yielding on average 47.6 million reads per sample.