HCV38
Homo sapiens
Protocols: Peripheral blood of HCV patients was collected in CPT tubes. As control, the blood of twelve healthy donors (HD) without any hematological abnormalities was collected for scientific purposes Genomic DNA of PBMCs and fresh frozen liver tissue was isolated using the GenElute mammalian genomic DNA miniprep kit (Sigma-Aldrich, Taufkirchen, Germany) rearranged IGH loci were amplified from 500 ng of blood or tissue-derived genomic DNA using a multiplex PCR, pooled at 4 nM and quality-assessed on a 2100 Bioanalyzer