Sample 1
Homo sapiens
Protocols: Spheroid were labelled for 2 hours with Calcein-AM and Vybrant-DiD both at 10uM at 37C. The spheroids were collected, washed twice in PBS, digested for 5 minutes with TrypsinLE 0.25% and quenched Spheroid culture media. The cell suspension was washed twice with PBS and live single cells were sorted according to the smartseq3xpress protocol. smartseq3xpress protocol smartseq3xpress protocol