Sample 1
Arabidopsis thaliana
Protocols: Flowers were collected from mature plants and immediately frozen Plants were grown in soil-filled pots in long day conditions (16h light) at approximately 120 umol s-1 m-2. Mitoribosome footprints were prepared from Arabidopsis flower buds as previously described (Planchard et al., 2018). Ribosome footprints were depleted from ribosomal RNAs after first strand cDNA synthesis using custom biotinylated oligonucleotides matching major rRNA contaminants found in previous experiments (Planchard et al., 2018). N. Planchard, P. Bertin, M. Quadrado, C. Dargel-Graffin, I. Hatin, O. Namy, H. Mireau, The translational landscape of Arabidopsis mitochondria. Nucleic acids research 46, 6218-6228 (2018). Ribo-Seq libraries were prepared using the TruSeq Small RNA library preparation kit (Illumina).