First whole instar_Replicate-3
Lucilia cuprina
Pool of 10 to 15 whole larvae; all samples with exception of L1_4 and 5 and L2_4 and 5, were from Dr. Scott L. cuprina cuprina colony which is currently ~ 170 generations and was established in 2010 using 300 individuals from the L. cuprina LA07 colony (~ 30 generations in 2010).  The LA07 L. cuprina colony was established by multiple collections of individuals (~ 300 to 500) from USC campus and the Miracle Mile neighborhood, in Los Angeles, CA, USA by Dr. Tarone (Texas A&M, TX, USA) in 2007. L1_4 and 5 and L2_4 and 5 samples were heterozygous individuals obtained from a cross between the LA07 colony and the SLAM5X colony which carries a DsRed fluorescent marker plus a Chclam promoter driving tTA linked to the X chromosome.