S_1605
Homo sapiens
Protocols: ER- BC patients with ductal carcinoma who underwent curative tumour resection surgery in Glasgow Royal Infirmary, Western Infirmary, or Stobhill Hospitals (Glasgow, UK) between 1995 and 1998 were included in the study. Resected tumour tissue was formalin-fixed and paraffin-embedded. FFPE tissue was deparaffinised prior to tissue digestion. The tissue lysate was combined with detector oligos which were annealed in immediate juxtaposition to each other on the targeted RNA template and ligated. Amplification of ligated oligos was performed using a unique primer set for each sample, introducing a sample-specific barcode and Illumina adaptors.