Zea mays
For this transcriptome study, various tissue were dissected from mature plants of ZnC442 × SCML0849 hybrid then stored in liguid nitrogen. Experiments were performed in three biological replicates each tissue consisting of 5 plants resulting in 27 distinct samples (9 tissue x 3 replicates). The total RNA of captured cells was isolated with trizol agent (Invitrogen). RNA quality was assessed via a Bioanalyzer (Agilent RNA 6000 Nano Chip, Agilent Technologies, Santa Clara, USA). For all samples, a RIN ≥ 6.5 detected. cDNA libraries for Illumina sequencing were constructed according to the protocol of the manufacturer (TruSeq RNA Sample Preparation, Illumina, San Diego CA, USA). For sequencing, each library was indexed by an Illumina TruSeq Adapter in which no adapter was used more than twice. Indexed libraries were loaded onto a flow cell following an incomplete block design with four pooled libraries per lane. Cluster preparation and pair-end read sequencing were performed according to the manufacturer's instructions (HiSeq 4008, Illumina, San Diego CA, USA).