sequencing data analysis of C. albicans CHS1 gene by PrimeSTAR amplification
Candida albicans
The C. albicans SC5314 chitin synthase (CHS1) gene (Gene ID: 3641364) was specifically targeted using PrimeSTAR Max DNA Polymerase (TaKaRa, Beijing, China). And DNA amplification products directly were detected by high-throughput sequencing. Both RPA and PCR products were amplified with adapters and barcodes suitable for Illumina NovaSeq sequencing. Raw reads were obtained from Tsingke Biotechnology (Beijing, China) and analyzed using Python scripts (Python 3.11.8), filtering reads with an average Phred quality (Q score) of at least 25.