3D-HT-29-Dabra-RepC-Barcode
Homo sapiens
Protocols: Cell lines were trypsinized and pelleted after they become resistant. Genomic DNA isolation was performed using GeneJET Genomic DNA isolation kit (ThermoFisher, U.S.A.) according to manufacturer's recommendations. For barcode sequencing, Library preparation protocol is based on two rounds of PCR to amplify the integrated barcodes from the genomic DNA and carried out according to manufacturer's guidelines provided by the Cellecta. Ten whole exome sequencing libraries were prepared from genomic DNA by using Twist Comprehensive Exome kit according to the manufacturer's instructions.