Homo sapiens
Protocols: To investigate synovial tissue myeloid DC and T-cell heterogeneity in RA, we performed scRNAseq on matched peripheral blood mononuclear cells (PBMC) and synovial tissue (ST) biopsies (n=3). Synovial biopsies were taken using 14G Precisa Needle (HS Hospital Service, Italy) in ultrasound-guided protocol, and digested as described previously. All live cells or CD45pos immune cells, were FACS sorted, after exclusion of dead cells with Fixable Viability Dye eFluor® 780 (eBiosciences). Maximum 20,000 immune cells from blood or tissue cells were sorted into Protein LoBind 1.5 ml Eppendorf tube containing 300 μl of RPMI media with 10% of FSC. Cells were loaded onto a Chromium Controller (10X Genomics) for single-cell partitioning, followed by library preparation using Single-Cell 3' Reagent Kits v2.  As recommended by 10X Genomics protocol for the 10X Single Cell 3' Reagent Kit v2 As recommended by 10X Genomics protocol for the 10X Single Cell 3' Reagent Kit v2
ENA first public
2024-12-09
INSDC center name
School of Infection & Immunity, University of Glasgow
INSDC status
public
Submitter Id
E-MTAB-14192:SA271_ST
age
77
broker name
ArrayExpress
cell_type
all synovial celltypes
collection date
not collected
common name
human
developmental stage
adult
disease
rheumatoid arthritis
geographic location (country and/or sea)
not collected
individual
SA271
isolate
not applicable
organism part
blood
response to treatment
Naive to treatment
sample name
E-MTAB-14192:SA271_ST
scientific_name
Homo sapiens
sex
female