Homo sapiens
Protocols: The brain samples and/or bio samples were obtained from the Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam (open access: www.brainbank.nl). All Material has been collected from donors for or from whom a written informed consent for a brain autopsy and the use of the material and clinical information for research purposes had been obtained by the NBB. Additional samples were provided by the Queen Square Brain Bank of Neurological Disorders and MRC, King College London. We complied to all relevant regulations of the mentioned brain banks for the work with these samples. Total RNA for RNA-seq was isolated from ±100mg of frozen brain tissue with TRIzol reagent (Thermo Fischer Scientific) according to the manufacturer recommendation, followed by purification with the RNeasy mini columns (Qiagen) after DNAse treatment. Total RNA-seq libraries were prepared from 1 microgram of total RNA from frozen brain tissue using the TruSeq Stranded Total RNA with Ribo-Zero Gold kit (Illumina) according to the protocol specifications.
ENA-FIRST-PUBLIC
2023-02-17
ENA-LAST-UPDATE
2023-02-17
External Id
SAMEA112484889
INSDC center alias
German Center for Neurodegenerative Diseases (DZNE)
INSDC center name
German Center for Neurodegenerative Diseases (DZNE)
INSDC first public
2023-02-17T00:21:52Z
INSDC last update
2023-02-17T00:21:52Z
INSDC status
public
Submitter Id
E-MTAB-12647:rimod38
age
64
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
frontotemporal dementia
genotype
C9orf72 mutation
individual
rimod38
organism part
frontal lobe
sample name
E-MTAB-12647:rimod38
scientific_name
Homo sapiens
sex
female