Danio rerio
Protocols: FACS-sorting of cd45:Dsred cells. Brains from adult Tg(p2ry12:GFP; cd45:DsRed) fish (n=3), were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline (DPBS) were triturated and treated with Liberase TM at 33°C for 30-45 minutes, fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis, using calcein violet to exclude dead cells (1µM, Thermo Fisher). Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II (Becton Dickinson). RNA Illumina
ENA-FIRST-PUBLIC
2023-12-25T01:15:29Z
ENA-LAST-UPDATE
2023-12-25T01:15:29Z
External Id
SAMEA114235870
INSDC center name
Universite Libre de Bruxelles
INSDC first public
2023-12-25T01:15:29Z
INSDC last update
2023-12-25T01:15:29Z
INSDC status
public
Submitter Id
E-MTAB-13223:Sample 1
age
5
broker name
ArrayExpress
cell_type
leukocyte
collection date
not collected
common name
zebrafish
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
pool of 3 fish
isolate
not applicable
organism part
brain
sample name
E-MTAB-13223:Sample 1
scientific_name
Danio rerio
sex
male and female
strain
AB