Mus musculus
Protocols: Naive murine embryonic stem cells (mESC) were derived freshly (FVB or mixed 129/B6) and were routinely cultured on gelatin-coated plates in t2i/L media: NDiff (N2B27) (Takara #y40002) supplemented with titrated 2i (0.2 uM PD0325901 and 3 uM CHIR 99021), 1,000 U/ml leukaemia inhibitory factor (LIF), 1% FBS, 1% penicillin streptomycin and maintained in humidified atmosphere at 37C and 5% CO2. Cells were passaged every 2 days by dissociation with TrypLE and medium was changed daily. RNA-sequencing of total RNA from mouse Embryonic Stem Cells. Total RNA was isolated using the Monarch. Total RNA Miniprep Kit (NEB #T2010S), including gDNA removal step and on-column DNase I digestion. Total RNA was quantified using Qubit II and quality assessed with high-sensitivity RNA Screen Tape (Agilent 5067-5579). rRNA was depleted using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (cat.no. E7400X) Stranded RNA-seq libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (cat.no. E7760L) (input: 500 ng of total RNA) following the manufacturer's instructions.
ENA-FIRST-PUBLIC
2023-11-15T10:00:40Z
ENA-LAST-UPDATE
2023-11-15T10:00:40Z
External Id
SAMEA114497945
INSDC center name
EMBL
INSDC first public
2023-11-15T10:00:40Z
INSDC last update
2023-11-15T10:00:40Z
INSDC status
public
Submitter Id
E-MTAB-13468:21-028_7_ESC_SN_aza
broker name
ArrayExpress
cell_line
mESC
cell_type
ESC
collection date
not collected
common name
house mouse
genetic modification
gene knock out
genotype
homozygous setd2 KO
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-13468:21-028_7_ESC_SN_aza
scientific_name
Mus musculus
sex
female
strain
FVB