plastisphere metagenome
LDPE incubation - pelagic habitat - 10 months
ENA first public
2024-04-12
INSDC center name
Royal NIOZ
INSDC status
public
Submitter Id
NIOZ197.069.064
ammonium (mg/L)
<0.04
broad-scale environmental context
Mediterranean Sea Biome [ENVO:01000047]
chlorophyll (µg/L)
<0.1
collection date
2015-06-06
depth
20
dissolved organic carbon (mg/L)
1.6
dissolved oxygen (mg/L)
7.96
environmental medium
Sea Water [ENVO:00002149]|Plastisphere [ENVO:06105106]
experimental factor
Field incubation, Time series
geographic location (country and/or sea)
Mediterranean Sea
geographic location (latitude)
42.5779
geographic location (longitude)
10.1078
geographic location (region and locality)
Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago
local environmental context
Coastal zone [ENVO:00000486]
multiplex identifiers
FWD (Golay69): GAAATCTTGAAG; REV (BC64): CACAGTTGAAGT
nitrate (mg/L)
0.02
nitrite (mg/L)
<0.02
nucleic acid extraction
Powersoil DNA Isolation kit
organic carbon (mg/L)
1.6
pH
8.2
particulate organic carbon (mg/L)
not collected
pcr conditions
Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min
pcr primers
FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT
phaeopigments (µg/L)
2.3
phosphate (mg/L)
<0.005
polymer disintegration (%)
0
project name
Open-Bio bioplastics
salinity
37.9
sample name
NIOZ197.069.064
scientific_name
plastisphere metagenome
sequence quality check
software
sequencing method
Illumina MiSeq
silicate (mg/L)
<0.4
target gene
16S/18S SSU rRNA
targeted amplicon size
400bp, 600bp
temperature
13.7
total depth of water column
40
total inorganic nitrogen (mg/L)
not collected
total nitrogen (mg/L)
0.34
total phosphorus (mg/L)
0.01