synthetic construct
Negatively supercoiled plasmid DNA used in DNA nicking assays to investigate the activation of MutLgamma by EXO1 and other cofactors (MutSgamma, RFC-PCNA). Purified human recombinant proteins were incubated with the plasmid, and GLOE-seq was performed to map each 3? OH end at nucleotide resolution, revealing widely distributed incision points.
ENA first public
2025-04-10
INSDC center name
IRB
INSDC status
public
Submitter Id
GLOE-seq_Figure 1
collection date
not applicable
geographic location (country and/or sea)
Switzerland
sample name
GLOE-seq_Figure 1
scientific_name
synthetic construct