Homo sapiens
Protocols: U2OS cells were grown in DMEM medium containing 10% foetal bovine serum at 37°C incubator with 5% CO2. After reverse cross-link and protein digestion with Proteinase K (Roche), DNA was purified using Qiaquick, PCR purification kit (Qiagen) following the manufacturer's. ChIP-seq was performed as described in Schmidt et al., 2009, using 3 x 10^6 U2OS. Immuno-precipitation was performed overnight at 4° C by incubating the shared DNA-protein complex with3 micro-gram of anti-SUMO2 (Life technologies) antibody previously coupled to Dynabeads protein A (Invitrogen). Schmidt D, Wilson MD, Spyrou C, Brown GD, Hadfield J, Odom DT. (2009) ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions. Methods 48: 240–248. Libraries were generated following manufacturer's protocol.
ENA-FIRST-PUBLIC
2023-06-01
ENA-LAST-UPDATE
2023-06-01
External Id
SAMEA111468662
INSDC center alias
Faculty of Biology, Medicine and Health, University of Manchester
INSDC center name
Faculty of Biology, Medicine and Health, University of Manchester
INSDC first public
2023-06-01T00:15:25Z
INSDC last update
2023-06-01T00:15:25Z
INSDC status
public
Submitter Id
E-MTAB-12293:SUMO2 starved U2OS
broker name
ArrayExpress
cell_line
U2OS
common name
human
developmental stage
juvenile stage
disease
osteosarcoma
environmental stress
nutrient starvation
genotype
wild type genotype
isolate
not applicable
organism part
tibia
sample name
E-MTAB-12293:SUMO2 starved U2OS
scientific_name
Homo sapiens