Pantoea agglomerans
Strain is HiVir- (non-pathogenic on onions) and Alt-. Small canes (diameter < 5mm) from a plant of Rubus sp. with disease symptoms, i.e. water-soaked and necrotic tissue, were collected near Ithaca, NY in the morning, placed in resealable plastic bags, and transported to the lab. The sample was cut into an approximately 3cm long piece and placed into a petri dish filled with 70% ethanol for 120 seconds. The piece was removed and allowed to air dry. In a laminar flow hood, a small portion of the stem that was connected to the petiole, including the parts of the petiole and leaf without necrosis, were cut into <5mm pieces using sterilized scissors and placed directly onto a plate of KB media and placed into an incubator at 28C for 40 hours. Cutting above the petri dish helped ensure that the pieces would fall onto the plate. The plate was dominated by a pale mucoid colony and was restreaked onto a plate with LB media and incubated at 28 C for 2 days. The original pale colony was a mixture of two colonies, which were streaked separately on LB plates and incubated at 28 C for 2 days. Two PCR reactions were performed using GYRB 1480F/2242R (Bonasera, et al., 2014) and 8UA/T5A (Bonasera, et al., 2017) primers. PCRs were cleaned and sent for Sanger Sequencing at the Cornell University Biotechnology Resource Center (BRC), Ithaca, NY. Strains were putatively identified as Pantoea agglomerans and Pseudomonas sp. The P.agglomerans strain was pursued for further investigation.
strain
ROTS050421
isolation_source
Rubus sp.
collection_date
2021
geo_loc_name
USA: New York
sample_type
cell culture
biomaterial_provider
Paul Stodghill, USDA-ARS
collected_by
Stephen Stresow; Jo Ann Asselin
env_broad_scale
area of developed open space [ENVO:01000883]
host_tissue_sampled
stem [BTO:0001300]
identified_by
Stephen Stresow, Jo Ann Asselin
BioSampleModel
Microbe, viral or environmental