Clostridium butyricum
Protocols: samples were subcultured on columbia cystein agar at 37°C for 48 hours, and then cultured in 10 mL BHI broth firstly and 20 mL BHI broth secondly overnight at 37°C prior to isolation. All cultures have been perfomed in an anaerobic chamber (Coy Laboratory Products Inc, Grass Lake, MI, USA) (v/v: 90% N2, 5% H2, 5% CO2). Clostridium butyricum cultures were incubated until it reached an optical density (OD600) of 1.1 for isolation at mid-exponential phase. Total bacterial RNA was then extracted and purified using ZYMO Direct-zol™ RNA Miniprep Plus R2070 extraction kit and treated with DNAse I according to manufacturer's instructions. A total of 100 ng RNA sample was used to construct each RNAseq library using Illumina stranded Total RNA Ligation Ribo0+ kit and subjected to ribosomal RNA (rRNA) depletion following manufacturer's instructions
ENA first public
2025-04-10
INSDC center name
IP
INSDC status
public
Submitter Id
E-MTAB-14532:HBD2
broker name
ArrayExpress
collection date
not collected
genotype
mutant
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-14532:HBD2
scientific_name
Clostridium butyricum