Homo sapiens
Protocols: Growth conditions - This submission studied the effect of IFNγ stimulation on 6 cancer cell lines. A375 (https://www.cellosaurus.org/CVCL_0132) and 501-mel (https://www.cellosaurus.org/CVCL_4633) were human melanoma cell lines. B16-F10 (https://www.cellosaurus.org/CVCL_0159), 5555 (provided by Santiago Zelenay, The University of Manchester) and YUMM2.1 (https://www.cellosaurus.org/CVCL_JK30) mouse melanoma cell lines. MC38 (https://www.cellosaurus.org/CVCL_B288) mouse colon cancer cell line. Human cell lines were maintained in DMEM (high glucose) medium. Mouse cell lines were maintained in RPMI-1640 (Sigma-Aldrich) medium. All growth media were supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich). All cells were maintained under standard conditions at 37°C in a 5% CO2 humidified incubator and passaged before reaching confluency. Cell cultures were routinely tested for mycoplasma contamination by PCR. Cells were passaged with or without IFNγ for at least 2 weeks, samples are then harvested for RNA-seq. Human cells (A375) were treated with 20 IU/ml IFNγ, mouse cells with 50 IU/ml IFNγ, replacing medium and IFNγ every 72 h with passaging of cells as necessary for 2 weeks. Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco. Growth conditions - This submission studied the effect of IFNγ stimulation on 6 cancer cell lines. A375 (https://www.cellosaurus.org/CVCL_0132) and 501-mel (https://www.cellosaurus.org/CVCL_4633) were human melanoma cell lines. B16-F10 (https://www.cellosaurus.org/CVCL_0159), 5555 (provided by Santiago Zelenay, The University of Manchester) and YUMM2.1 (https://www.cellosaurus.org/CVCL_JK30) mouse melanoma cell lines. MC38 (https://www.cellosaurus.org/CVCL_B288) mouse colon cancer cell line. Human cell lines were maintained in DMEM (high glucose) medium. Mouse cell lines were maintained in RPMI-1640 (Sigma-Aldrich) medium. All growth media were supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich). All cells were maintained under standard conditions at 37°C in a 5% CO2 humidified incubator and passaged before reaching confluency. Cell cultures were routinely tested for mycoplasma contamination by PCR. Cells were passaged with or without IFNγ for at least 2 weeks, samples are then harvested for RNA-seq. Human cells (A375) were treated with 20 IU/ml IFNγ, mouse cells with 50 IU/ml IFNγ, replacing medium and IFNγ every 72 h with passaging of cells as necessary for 2 weeks. Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco. Total RNA was isolated using the RNAeasy mini kit. RNA integrity was assessed on the Agilent 2200 TapeStation (Agilent Technologies). TruSeq Stranded mRNA assay (Illumina)
isolate
not applicable
disease
melanoma
ENA-FIRST-PUBLIC
2023-08-22T20:40:49Z
organism
Homo sapiens
organism
Homo sapiens
ENA-LAST-UPDATE
2023-08-22T20:40:49Z
cell_line
501-mel
scientific_name
Homo sapiens
common name
human
organism part
skin
ENA-FIRST-PUBLIC
2023-08-22
ENA-LAST-UPDATE
2023-08-22