Homo sapiens
Protocols: A549 cells (CCL-185 on ATCC) were cultured in DMEM medium (Gibco, DMEM, low glucose, GlutaMAX™ Supplement, pyruvate, Catalog number: 21885025) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS), Penicillin (100 U/mL) and Streptomycin (100 U/mL) in a humidified incubator at 37°C; 5% CO2. 10 micromolar miR-200c mimic library or scrambled control. Total RNA from cells was extracted using the RNAdvance Cell kit (Beckman Coulter). RNA extraction was performed using Biomek i7 robotic handler (Beckman Coulter) following manufacturer instructions 55 ng of total RNA were used for RNA-seq library preparation using the KAPA mRNA HyperPrep Kit (Roche) following manufacture's instruction (KAPA Biosystem, Illumina Platforms) proceeded by TECAN Fluent liquid handing automation system (Tecan). The library was amplified for 14 cycles and quantified using the Fragment Analyzer (Advanced Analytical) with the standard sensitivity NGS kit (Agilent technologies) followed by the library clean-up with 1x volume of AMPure XP beads (Beckman Coulter Life Sciences). All the libraries were pooled and diluted into 1.8 nM quantified using Qubit Fluorometer (ThermoFisher Scientific)
ENA-FIRST-PUBLIC
2023-02-17
ENA-LAST-UPDATE
2023-02-17
External Id
SAMEA112484143
INSDC center alias
AstraZeneca, UK
INSDC center name
AstraZeneca, UK
INSDC first public
2023-02-17T00:21:52Z
INSDC last update
2023-02-17T00:21:52Z
INSDC status
public
Submitter Id
E-MTAB-12574:Sample 31
broker name
ArrayExpress
cell_line
A549
common name
human
developmental stage
adult
disease
lung cancer
genotype
wild type genotype
organism part
lung epithelium
sample name
E-MTAB-12574:Sample 31
scientific_name
Homo sapiens