Trypanosoma brucei brucei
SL_SS3x scRNA-seq of T. brucei Lister 427 strain. Multiple samples:  (1) Cell line with Tet-inducible Cas9 and the constitutive expression of a sgRNA targeting VSG-2 CDS between nucleotides 766-785. Time course (0hrs,24hrs,48hrs,72hrs,96hrs,10days) after Cas9-mediated DSB induction at nucleotide position 782 of VSG-2 CDS. (2) Cell line with Tet-inducible Cas9 and the constitutive expression of a sgRNA targeting between nucleotides 59993-60012 of BES1 (BES1_Tb427v11), positions 40209-40228 relative to the promoter. 0hrs and 96hrs time points after Cas9-mediated DSB induction at nucleotide position 40225 downstream of BES1 promoter. (3) Cell line with Tet-inducible Cas9 and the constitutive expression of a sgRNA targeting between nucleotides 70023-70042 of BES1 (BES1_Tb427v11), positions 50209-50228 relative to the promoter. 0hrs and 96hrs time points after Cas9-mediated DSB induction at nucleotide position 50225 downstream of BES1 promoter. (4) Cell line with Tet-inducible Cas9 and the constitutive expression of a sgRNA targeting between nucleotides 74592-74611 of BES1 (BES1_Tb427v11), positions 54808-54827 relative to the promoter. 0hrs and 96hrs time points after Cas9-mediated DSB induction at nucleotide position 54824 downstream of BES1 promoter. (5) Cell line with Tet-inducible Cas9 and the constitutive expression of a sgRNA targeting between nucleotides 77917-77936 of BES1 (BES1_Tb427v11), positions 58133-58152 relative to the promoter. 0hrs and 96hrs time points after Cas9-mediated DSB induction at nucleotide position 58149 downstream of BES1 promoter. (6) Cell line with Tet-inducible Cas9 and the constitutive expression of a sgRNA targeting between nucleotides 593-612 of VSG-8 CDS. 0hrs and 96hrs time points after Cas9-mediated DSB induction at nucleotide position 609 of VSG-8 CDS. (7) Cell line with Tet-inducible Cas9 and the constitutive expression of a sgRNA targeting between nucleotides 1089-1108 of VSG-8 CDS. 0hrs and 96hrs time points after Cas9-mediated DSB induction at nucleotide position 1105 of VSG-8 CDS.
organism
Trypanosoma brucei brucei
collection date
2023
scientific_name
Trypanosoma brucei brucei
geographic location (country and/or sea)
Germany