Mycolicibacterium smegmatis MC2 155
5 µg total RNA of M. smegmatis with 1 mM CTP were incubated with water or 10 µg MenT1 WT for 1 h at 37 °C, To remove m1A, m3C and m1G modification in the tRNAs, the total RNA samples were pretreated using the demethylase kit (Arraystar, cat#: AS-FS-004, Rockville, MD, USA), followed by trizol isolation.
isolate
culture
ENA-FIRST-PUBLIC
2023-06-01
organism
Mycolicibacterium smegmatis MC2 155
ENA-LAST-UPDATE
2023-06-01
scientific_name
Mycolicibacterium smegmatis MC2 155
ENA-FIRST-PUBLIC
2023-06-01
ENA-LAST-UPDATE
2023-06-01