Mus musculus
Protocols: Mouse splenic B cells were purified by negative selection and cultured in complete medium in the presence of in vitro differentiation cocktails (anti-IgM, R848, anti-CD40, IL-21 plus IFN-γ or IL-4) for 3 days. The in vitro dereived B cells were collected for RNA sequencing. The in vitro indued B cells were resuspended in TRIzol reagent for total RNA extraction. RNA integrity was assessed using the Fragment Analyzer 5400 (Agilent Technologies, CA, USA). Then total RNA was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
ENA-FIRST-PUBLIC
2023-02-17
ENA-LAST-UPDATE
2023-02-17
External Id
SAMEA112483522
INSDC center alias
Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai0, China, 20001.
INSDC center name
Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai0, China, 20001.
INSDC first public
2023-02-17T00:21:53Z
INSDC last update
2023-02-17T00:21:53Z
INSDC status
public
Submitter Id
E-MTAB-12569:IFN-γ-3
broker name
ArrayExpress
cell_type
in vitro derived B cells
common name
house mouse
growth condition
IFNg stimulation condition
sample name
E-MTAB-12569:IFN-γ-3
scientific_name
Mus musculus
strain
C57