microbial mat metagenome
St3 Schiermoniekoog Microbial mat
ENA first public
2024-12-26
External Id
SAMEA114321693
Forward barcode
GTTCATTAAACT
INSDC center name
Royal Netherlands Institute for Sea Research (NIOZ)
INSDC last update
2023-09-01T13:58:01Z
INSDC status
public
Reverse barcode
GAGCGCCGAACA
Submitter Id
NIOZ310.217.208
barcodenames
515F_Golay217 and 926RBC_Golay208
collection date
2010-04
forwardprimersequence
GTGYCAGCMGCCGCGGTAA
geographic location (country and/or sea)
Netherlands
geographic location (latitude)
29' 28.3626
geographic location (longitude)
6� 8' 20.9646
nucleic acid extraction
Dneasy Powersoil kit
pcr conditions
initial denaturation at 98 C for 30 s; 30 cycles of 98 C for 30 s, 50 C for 15 s, and 72 C for 30 s; final extension at 72 C for 5 minutes
pcr primers
Forward Primer (515F) GTGYCAGCMGCCGCGGTAA Reverse Primer (926R) CCGYCAATTYMTTTRAGTTT
project name
HglT primers
purificationmethod
AmpureBeads
reverseprimersequence
CCGYCAATTYMTTTRAGTTT
sample name
NIOZ310.217.208
scientific_name
microbial mat metagenome
sequencing method
Illumina MiSeq
target gene
16S rRNA
target subfragment
V4V5
targetedampliconsize
600bp400bp