Hordeum vulgare
Protocols: Barley crown tissue was sampled for gene expression analysis using mRNA-seq in three biological replications at two time points: 3 d, tillering stage (23-26 of BBCH code), and 10 d, ten days after the first day. Each replication consisted of crown samples collected from three plants per pot. Drought stress experiment was conducted in the growth chambers under fully controlled conditions. Seeds were sown in pots (H-LSR 4.5 L; 21 cm in diameter and 20 cm in height) filled with a mixture of loamy soil and peat (3:1, w/w) and plants were cultivated in optimal conditions: soil moisture above 70% filed water capacity (FWC), 22°C/16°C day/night, air humidity of 60-70%, a photoperiod of 16/8 h of light/dark. The number of pots was established so as to contribute with an adequate amount of plant material for all molecular studies along with phenotyping. Biological samples of barley crown tissue for molecular analyses were collected at early stage of drought (3rd day of stress, T1) and at the end of stress application – late drought (10th day of stress, T2). Each replication consisted of crown samples collected from three plants per one pot. Drought (D) was imposed at the tillering stage (23-26 of BBCH code) and maintained for 10 days. Water scarcity was established at 20% FWC and soil moisture in each pot was controlled gravimetrically by weighing and volumetrically (if necessary) using the FOM/mts device. In parallel, the optimal irrigation experiment (C) was carried out. Total RNA (1-2 µg) was extracted in triplicates using Qiagen RNeasy Plant Mini Kit (Hilden, Germany) according to manufacturer's instruction. Genomic DNA contamination was removed twice i.e. on-column DNase digestion (RNase-Free DNase Set, Qiagen) and using DNase Max Kit (Qiagen) during and after RNA isolation, respectively. Library was constructed using library preparation kit: Illumina TruSeq Stranded mRNA (with poly A selection).
ENA-FIRST-PUBLIC
2023-03-30
ENA-LAST-UPDATE
2023-03-30
External Id
SAMEA112776348
INSDC center alias
IGR-PAS
INSDC center name
Institute of Plant Genetics Polish Academy of Sciences
INSDC first public
2023-03-30T12:21:40Z
INSDC last update
2023-03-30T12:21:40Z
INSDC status
public
Submitter Id
E-MTAB-12773:Sample 34
age
4
broker name
ArrayExpress
cultivar
BW885
developmental stage
formation of axillary shoot stage
environmental stress
drought environment
genotype
wild type genotype
growth condition
long day length regimen
individual
92
organism part
crown root
sample id
BW885D13
sample name
E-MTAB-12773:Sample 34
scientific_name
Hordeum vulgare
tube id
73