Danio rerio
Protocols: Adult zebrafish were euthanized, kidney marrow was isolated and dissociated by pipetting in PBS/10% FCS. 7-AAD (7-amino-actinomycin D) 0.5mg/L (BD biosciences) was used for live/dead discrimination. Sorting was performed using FACSAriaIII (BD). 70.000 single viable cells were sorted from 2 pooled kidney marrow of female Tg(CD41:GFP) zebrafish and supplemented with 114-1607 CD41:GFPlow expressing cells. cDNA was prepared using the manufacturers protocol (10x Chromium V3). Library was prepared using the manufacturers protocol (10x Chromium V3).
ENA first public
2024-11-05
INSDC center name
Department of Hematology cancer institute ErasmusMC
INSDC status
public
Submitter Id
E-MTAB-14075:Het_young
age
12
broker name
ArrayExpress
cell_type
hematopoietic cell
collection date
not collected
common name
zebrafish
developmental stage
adult
genotype
Gata2bHet
geographic location (country and/or sea)
not collected
individual
pools of 2 fish
isolate
not applicable
organism part
kidney marrow
sample name
E-MTAB-14075:Het_young
scientific_name
Danio rerio
sex
mixed
strain
Tg(CD41:GFP; runx1:DsRed) Gata2b KO