Tetrahymena thermophila
A total of five million micronuclei (MICs) were purified from EMIT3 knock out Tetrahymena meiotic cells that expressing exogenous Emit3-CS-HA, then the MICs were used for the Cleavage Under Targets and Tagmentation (CUT&Tag) experiment for the identification of Emit3-CS binding regions in the meiotic MIC. CUT&Tag was conducted using BioMagPlus Concanavalin A beads (Bangs Laboratories, Fishers, IN), a commercial Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, Nanjing, China), and an anti-HA rabbit monoclonal antibody (Clone C29F4, Cell Signaling Technology). CUT&Tag libraries were generated using indexed primers and the KAPA HiFi HotStart Real-Time Library Amplification Kit (Roche) and subsequently sequenced on a NovaSeq 6001 platform (Illumina, San Diego, CA).
strain
emit3ko
dev_stage
C3
collection_date
2024-06-07
geo_loc_name
China
sex
pooled male and female
tissue
single cell culture
BioSampleModel
Model organism or animal