Mus musculus
Protocols: T-bet-tdTomato reporter mice were adoptive transferred of CD4+ T cells from Bm12 mice. After 14 days, ABCs (CD11c+T-bet+CD21- B cell) and non-ABCs (CD11c-T- bet- B cell) were sorted by FACSAria II. Total RNA was extracted using TRIzol reagent (15596018, Thermo Fisher) Total RNA was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.
ENA-FIRST-PUBLIC
2023-02-16
ENA-LAST-UPDATE
2023-02-16
External Id
SAMEA112474096
INSDC center alias
Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai0, China, 20001.
INSDC center name
Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai0, China, 20001.
INSDC first public
2023-02-16T00:15:58Z
INSDC last update
2023-02-16T00:15:58Z
INSDC status
public
Submitter Id
E-MTAB-12568:DN-1
broker name
ArrayExpress
cell_type
B cell CD11c-T-bet-
common name
house mouse
sample name
E-MTAB-12568:DN-1
scientific_name
Mus musculus