Homo sapiens
Protocols: Patients and tissue collection. for HC, presence of a normal liver (no steatosis or cirrhosis) on ultrasound evidence; for NAFLD was based on ultrasound evidence of fatty liver in the absence of other (viral, alcohol, metabolic) causes. Liver tissue was collected by a wedge liver biopsy from free edge of the liver lobe during laparoscopic cholecystectomy. The study protocol received institutional review board (IRB) approval before the beginning of the study, in accordance with The Code of Ethics of the Declaration of Helsinki, and informed consent was obtained from all patients. tissue samples were immediately stored in RNA-later for 24 hours at 4℃, and subsequently at -80℃. RNA isolation and quality controls. Approximately 10-20 mg of liver tissues were homogenized in 1 mL Trizol (Thermo Fisher Scientific, shanghai, China) on ice, incubated for 5 min, then added 200 μL chloroform. After low-temperature centrifugation, 500 μL isopropanol was used to precipitate RNA. The precipitate was washed with 75% ethanol (DEPC water configuration), and then dissolved in 30 μL DEPC water (shanghai Generay Biotech Co., Ltd, shanghai, China) after evaporating all ethanol. The RNA concentration, purity and integrity were measured by Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Beijing, China). RNA concentration, purity and RNA integrity number (RIN) were measured using an Agilent 2100 Bioanalyzer before sequencing. 2 μg of RNA meeting the requirements of RIN ≥ 7 and 28S/18S ≥ 1.0 were taken for library construction.
ENA-FIRST-PUBLIC
2023-11-14T17:08:33Z
ENA-LAST-UPDATE
2023-11-14T17:08:33Z
External Id
SAMEA112194780
INSDC center name
Affiliated Hospital of Zunyi Medical University
INSDC first public
2023-11-14T17:08:33Z
INSDC last update
2023-11-14T17:08:33Z
INSDC status
public
Submitter Id
E-MTAB-12445:Sample 24
age
56
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
normal
individual
HC6
isolate
not applicable
organism part
liver
sample name
E-MTAB-12445:Sample 24
scientific_name
Homo sapiens
sex
male