Sus scrofa
Protocols: Peripheral blood mononuclear cells (PBMC) were prepared from heparinised blood using Histopaque and frozen in foetal calf serum supplemented with 10% DMSO. Cells were defrosted into warm RPMI GlutaMAX HEPES media supplemented with 10% foetal calf serum, 1 mM sodium pyruvate, 50 µM 2-mercaptoethanol, 100 IU/mL penicillin and 100 µg/mL streptomycin (RPMI/10). Nucleic acid was extracted from one million cells using a MagMAX mirvRNA Total RNA extraction kit on a MagMAX™ Express-96 Deep Well Magnetic Particle Processor. 3'UTR sequencing
ENA-FIRST-PUBLIC
2023-02-17
ENA-LAST-UPDATE
2023-02-17
External Id
SAMEA112483356
INSDC center alias
Researcher
INSDC center name
Researcher
INSDC first public
2023-02-17T00:21:52Z
INSDC last update
2023-02-17T00:21:52Z
INSDC status
public
Submitter Id
E-MTAB-12608:18-915
age
15
broker name
ArrayExpress
cell_type
blood cell
clinical history
Sham
common name
pig
disease
African Swine Fever (ASF)
genotype
MHC homozygous inbred Large White Babraham
organism part
blood
phenotype
Non-recovered
sample name
E-MTAB-12608:18-915
scientific_name
Sus scrofa
sex
male