Pseudomonas aeruginosa UCBPP-PA14
Protocols: P. aeruginosa monocultures and co-cultures with A. fumigatus were harvested in the stationary phase of growth (triplicates) by aspirating the contents of the petri-dish well into a 15 ml falcon tube (passed over a 40 µm cell strainer) and combining this with an equal volume of RNA-later. Cultures stored in RNAlater were pelleted, resuspended in 1 mL RNA Bee, and RNA was extracted. Cultures stored in RNAlater were pelleted, resuspended in 1 mL RNA Bee, and RNA was extracted as described previously (https://doi.org/10.1073/pnas.1717525115). Microbial Genome Sequencing Center (MiGS) - DNAse Treatment, rRNA Depletion, Stranded Library Prep and sequencing
ENA-FIRST-PUBLIC
2023-03-30
ENA-LAST-UPDATE
2023-03-30
External Id
SAMEA112798675
INSDC center alias
UNIVERSITY OF CAMBRIDGE
INSDC center name
UNIVERSITY OF CAMBRIDGE
INSDC first public
2023-03-30T12:17:32Z
INSDC last update
2023-03-30T12:17:32Z
INSDC status
public
Submitter Id
E-MTAB-12786:Sample 3
broker name
ArrayExpress
genotype
wild type genotype
growth condition
monoculture
organism part
whole organism
sample name
E-MTAB-12786:Sample 3
scientific_name
Pseudomonas aeruginosa UCBPP-PA14