Mus musculus
Protocols: 3T3-L1 fibroblasts were washed 3x in PBS and lysed in 350 µL RLT buffer. Lysed samples were collected and stored at -80°C until further analysis 3T3-L1 adipocytes were seeded and differentiated in 12-well plates. RNA extractions were performed using the RNeasy Mini Kit (Qiagen 1152 #74104) 1 μg of total RNA was quality checked (RIN >7) using an Agilent Bioanalyser 2100 system and then used to construct barcoded sequencing libraries with Illumina's TruSeq Stranded mRNA Library Prep Kit following manufacturer's instructions
organism
Mus musculus
organism
Mus musculus
scientific_name
Mus musculus
common name
house mouse
organism part
embryo
developmental stage
embryo
genotype
wild type genotype
isolate
not applicable
ENA-FIRST-PUBLIC
2023-10-01T00:27:18Z
ENA-LAST-UPDATE
2023-10-01T00:27:18Z
growth condition
low media volume
cell_line
3T3-L1
progenitor cell type
fibroblast
cell_type
adipocyte
ENA-FIRST-PUBLIC
2023-10-01
ENA-LAST-UPDATE
2023-10-01