Homo sapiens
Protocols: day 50 organoids were taken out of media, and immediately treated with Trizol and homogenized and stored in -80C until further extraction RNA extraction for cerebral organoids was performed using Trizol extraction. Organoids were lysed directly in Trizol (Invitrogen, Trizol, Cat #15596018). RNA was isolated according to the manufacturer's instructions. Cells were directly sorted into 1mL of Trizol. RNA was extracted in the aqueous phase after addition of 200μL chloroform, precipitated with an equal volume of isopropanol and washed once in fresh 80% ethanol. The RNA pellet was re-suspended in 20μL ddH2O at 50C for 10 minutes and then kept on ice. RNA was stored at -20°C long term. PCR with Nextera overhangs
ENA first public
2025-04-10
INSDC center name
The Azrieli Center for Stem Cells and Genetic Research Department of Genetics The Alexander Silberman Institute of Life Sciences The Hebrew University of Jerusalem Edmond J. Safra Campus, Givat Ram
INSDC status
public
Submitter Id
E-MTAB-14543:FX-4
broker name
ArrayExpress
cell_line
FX52
cell_type
heterogenous composition from cerebral organoid differentation
collection date
not collected
common name
human
developmental stage
Day 50
disease
Fragile X Syndrome
genotype
FMR1 silencing by CGG expansion and hypermethylation
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
cerebral organoid
sample name
E-MTAB-14543:FX-4
scientific_name
Homo sapiens