Pseudomonas aeruginosa UCBPP-PA14
Protocols: Pseudomonas aeruginosa PA14 was precultured in SCFM2, and the OD600 was adjusted to 0.05 in 8 ml of SCFM2, in 6-well flat bottom cell culture plates. Gliotoxin (20 µM) or MeOH (solvent control) was added at log phase (optical density at 600 nm [OD600] = 0.3), and the cultures were incubated statically for an additional 4 h. 5 ml of each culture (triplicate) was then immediately added to an equal volume of RNA-later. Cultures stored in RNAlater were pelleted, resuspended in 1 mL RNA Bee, and RNA was extracted as described previously (https://doi.org/10.1073/pnas.1717525115). Microbial Genome Sequencing Center (MiGS) - DNAse Treatment, rRNA Depletion, Stranded Library Prep and sequencing
ENA-FIRST-PUBLIC
2023-03-30
ENA-LAST-UPDATE
2023-03-30
External Id
SAMEA112798775
INSDC center alias
UNIVERSITY OF CAMBRIDGE
INSDC center name
UNIVERSITY OF CAMBRIDGE
INSDC first public
2023-03-30T12:17:32Z
INSDC last update
2023-03-30T12:17:32Z
INSDC status
public
Submitter Id
E-MTAB-12785:Sample 1
broker name
ArrayExpress
genotype
wild type genotype
organism part
whole organism
sample name
E-MTAB-12785:Sample 1
scientific_name
Pseudomonas aeruginosa UCBPP-PA14