Mus musculus
Protocols: Whole mouse brains were dissected and rapidly frozen on dry ice. Whole brains were homogenized using a bullet blender and glass beads in 10 ml Tris buffer. Whole brains were homogenized using a bullet blender and glass beads in 10 ml Tris buffer. 600 ul of the homogenate was used for RNA extraction using the Qiagen RNAEasy mini kit according to the manufactories recommendations. The library was constructed using the Ion AmpliSeq™ Library Kit from Thermo Fisher. For this the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit primer panel from Thermo Fisher was used. All procedures wrer performed according to the recommended protocol.
ENA-FIRST-PUBLIC
2023-02-16
ENA-LAST-UPDATE
2023-02-16
External Id
SAMEA112471701
INSDC center alias
UU
INSDC center name
Uppsala University
INSDC first public
2023-02-16T00:16:00Z
INSDC last update
2023-02-16T00:16:00Z
INSDC status
public
Submitter Id
E-MTAB-12561:KO 1
age
8
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
SLC38A10 knockout
organism part
brain
sample name
E-MTAB-12561:KO 1
scientific_name
Mus musculus
sex
male
strain
BTBR mouse