Mus musculus
Protocols: mice were sacrificed by exsanguination, lungs collected and placed on ice, minced, digested in collagenase, elastase, dispase, DNAse, and Lich enzyme mix at 4oC, RBCs lysed, and passed through 30 micron filter, and single cells suspended and labeled with 10X Cellplex lipid-conjugated oligos. Dead cells were excluded using DAPI and flow cytometry with 50 micron nozzle which selected for smaller, immune cells. cDNA libraries were created from GEM using a 10X Chromium controller after loading 16,000 cells per well into 4 wells. cDNA libraries were created using the v3.3 protocol, the Dual Index TT and NN kits, and 16 cycles. Feature barcode libraries were created using a 10X accessory kit
ENA-FIRST-PUBLIC
2022-09-23
ENA-LAST-UPDATE
2022-09-23
External Id
SAMEA111348379
INSDC center alias
CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER
INSDC center name
CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER
INSDC first public
2022-09-23T00:24:47Z
INSDC last update
2022-09-23T00:24:47Z
INSDC status
public
Submitter Id
E-MTAB-12097:KOPBS
age
8 to 9
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
Cela1 -/-
individual
KO4780, KO4783, KO4782
organism part
lung
sample name
E-MTAB-12097:KOPBS
sex
female
strain
C57BL6