Streptomyces venezuelae
Protocols: RNA was isolated from Streptomyces venezeulae wild-type strain cultivated in 5 mL MYM liquid medium for 10, 15, or 20 hours (each time point in triplicate). Mycelia were collected by centrifugation, frozen, and stored at -70°C for subsequent RNA isolation. RNA from mycelia was isolated using the GeneJET RNA isolation kit (Thermo Fisher Scientific, US). The lysozyme concentration in the suspension buffer was increased to up to 10 mg/mL to improve the yield of mycelium lysis. The isolated RNA was digested with TURBO DNase I (Invitrogen, United States) and subsequently, purified and concentrated using a GeneJET RNA Cleanup kit (Thermo Fischer Scientific, US) RNA libraries were prepared for sequencing using standard Illumina protocols (NEBNext Ultra II Directional RNA Library Prep Kit). rRNA was removed using Ribo-Zero Ribosoma RNA Removal Kit (Illumina)
ENA-FIRST-PUBLIC
2023-12-24T00:26:43Z
ENA-LAST-UPDATE
2023-12-24T00:26:43Z
External Id
SAMEA114856161
INSDC center name
Facultyof Biotechnology, University of Wrocław
INSDC first public
2023-12-24T00:26:43Z
INSDC last update
2023-12-24T00:26:43Z
INSDC status
public
Submitter Id
E-MTAB-13607:JM_003
broker name
ArrayExpress
collection date
not collected
developmental stage
vegetative
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
whole organism
replicate
3
sample name
E-MTAB-13607:JM_003
scientific_name
Streptomyces venezuelae