Small-scale, glucose-limited fed-batch-like screening. Parent strain expressing CRISPR-activation machinery and a scgRNA targetted to 783398-783420 on chr1. Target Sequence: GTTGGCGGTAGTAACTTGGGTGG.
Komagataella phaffii
strain
CBS:2612
isolation_source
Generated in this study. CRISPR activation machinery overexpressed in Parent vHH strain
collection_date
2023
geo_loc_name
Austria: Vienna
sample_type
whole organism
replicate
3
genetic_mod
pG1-3_VHH, dflo8, pTEF2_dCAS9(3xSV40NLS), pPOR1_MS2-VP64-SV40NLS, pGAP_scgRNA-chr1_783398-783420
type-material
type material of Komagataella phaffii
BioSampleModel
Microbe, viral or environmental