Metagenome-assembled genome: ERR13917544_binner12_Refined_49
uncultured bacterium
ENA first public
2025-06-17
INSDC center name
EMG
INSDC status
public
Submitter Id
ERR13917544_binner12_Refined_49
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaspades_v3.15.5
binning parameters
default
binning software
MGnify-genomes-generation-pipeline_v1.0.0
broad-scale environmental context
marine sediment
broker name
EBI-EMG
collection date
2017-04-19
completeness score
95.66
completeness software
CheckM2_v1.0.1
contamination score
0.55
environmental medium
marine sediment
geographic location (country and/or sea)
North Sea
geographic location (latitude)
54.08762700
geographic location (longitude)
7.96819100
investigation type
metagenome-assembled genome
isolation_source
sediment metagenome
local environmental context
protein degradation
metagenomic source
sediment metagenome
project name
An incubation experiment was performed, investigating protein degradation by uncultivated Thermoplasmatota in marine anaerobic sediments of the Helgoland mud area. Slurry for the first generation was prepared in a 1:4 ratio of sediment and sulfate-free artificial sea water. The used sediment originated from core HE483 station 2-2, 45-70 cm and was retrieved by gravity coring in 2017. The incubation experiment was performed anoxically for two treatments: the control sample was amended with 20 mM sulfate (Na2SO4) and an antibiotics mix (D-cycloserin, kanamycin, vancomycin, ampicillin and streptomycin 50 mg/l for each), the protein amended sample was additionally amended with 1.86 g/l egg white protein. After 372 days 5 mL of the first generation incubations were transferred into anoxic Widdel medium. The incubation experiment was performed anoxically for two treatments in duplicates: the control samples were amended with 30 mM sulfate (Na2SO4) and an antibiotics mix (D-cycloserin, kanamycin, vancomycin, ampicillin and streptomycin 40 mg/l for each), the protein amended samples were additionally amended with 2.33 g/l egg white protein. Sampling of the second generation was performed after 98 and 157 days. DNA was extracted and the present and active archaeal community was identified by 16S rRNA gene sequencing.
sample derived from
SAMEA116057598
sample name
ERR13917544_binner12_Refined_49
scientific_name
uncultured bacterium
sequencing method
Illumina HiSeq 4000
taxonomic identity marker
multi-marker approach