Mus musculus
isolate
Islets from male mice were isolated at 20 weeks of age by injecting 0.5 mg/mL collagenase Type V solution (C9263, Sigma-Aldrich, St. Louis, MO) into the pancreatic bile duct. Partially dissociated tissue was fractionated using a Histopaque-1077 (Sigma) gradient followed by hand-picking of islets. Islets from 20-week-old Osgep-Ins2-Cre_2 mice were used for separate RNA sample isolations.
age
20 weeks old
dev_stage
adlut
collection_date
2022-05-13
geo_loc_name
China:Changsha
sex
male
tissue
islet
replicate
replicate = biological replicate 2
BioSampleModel
Model organism or animal