Slide2
Homo sapiens
Protocols: Tissues were fixed in 10% neutral buffered formalin and embedded in paraffin. FFPE tissue sections were cut at a thickness of 5 µm and placed on glass slides. Hematoxylin and eosin (H&E) staining was performed according to the 10x Genomics protocol. After staining, the slides were imaged at 20x magnification using a VS200 slide scanner (Evident Corp., Tokyo, Japan). RNA was transferred to Visium HD slides using the CytAssist. cDNA synthesis and library construction were performed using the Visium HD Library Preparation Kit (10x Genomics) according manufacture's protocol.