In vitro transcription using pol1_Rpa12dC_2
Saccharomyces cerevisiae
Nanopore sequencing of in vitro transcribed RNA. Transcription reactions were performed on tailed template t.t#7 using 0.2 pmol Pol I RpaA12_C in the presence of 200 �M ATP, CTP, and UTP. Transcripts were isolated and sequenced using Oxford Nanopore Technologies. The dataset includes two biological replicates per condition. Replicate 2.