WT_F1_pollen2
Arabidopsis thaliana
Protocols: To purify pollen grains from F1 hybrid plants and extract their genomic DNA, whole inflorescences of hybrid plants were collected and transferred to ice-cold 10% (w/v) sucrose in a 50-ml tubes. Flowers were homogenized with a blender (Tefal, BL3051KR), filtered through 80-μm nylon mesh, and centrifuged at 350g for 10 min at 4 °C. The supernatant was discarded and the pellet was resuspended in 10% (w/v) sucrose, filtered through a 40-μm cell strainer, and centrifuged at 100g for 10 min at 4°C. The purified pellet was frozen in liquid nitrogen. long day conditions (16 hr light), 20 degrees Celcius DNA was extracted using the CTAB method. Nanobind disk (Nanobind Plant Nuclei Kit RT, PacBIO, 102-302-000) was used to collect high molecular weight genomic DNA. Ligation Sequencing Kit V14 (Nanopore, SQK-LSK114)