NusA IP WT 1
Escherichia coli str. K-12 substr. MG1655
Protocols: Bacterial strain MG1655 is a laboratory strain of Escherichia coli.  Cells were grown in liquid LB minimal medium at 37 degrees with shaking. Immunoprecipitations were done as described by Haycocks et al. (PLoS Pathog. 2015. 11:e1004605) using Vibrio cholerae strain MG1655. After growing cells to midlog phase, crosslinking, and sonication, protein DNA complexes were immunoprecipitated using anti-NusA or anti-Rho. Libraries were prepared using immunoprecipited protein-DNA complexes immobilised with Protein A-sepharose. DNA fragments were then blunt ended, A-tailed and barcoded. This was done using an NEB quick blunting and ligation kit, the Klenow fragment (5'-3' exo-, NEB) and NEXTflex ChIP-seq barcodes (Bioo Scientific). Following elution of complexes from the Protein-A sepharose, crosslinks were reversed and barcoded libraries were amplified by PCR. The number of PCR cycles was determined empirically for each library. After amplification, library concentration was quantified using Qubit analysis and real time PCR.
ENA first public
2026-02-19
INSDC center name
University of Birmingham
INSDC status
public
Submitter Id
E-MTAB-16394:NusA IP WT 1
broker name
ArrayExpress
collection date
not collected
genotype
wild type genotype
geographic location (country and/or sea)
not collected
growth condition
mid log phase
isolate
not applicable
replicate
1
sample name
E-MTAB-16394:NusA IP WT 1
scientific_name
Escherichia coli str. K-12 substr. MG1655
strain
K-12
substrain
MG1655