Metagenome-assembled genome: binchicken_co104_28
uncultured Bacteroidales bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs DRR182650,DRR182651 of study DRP005178.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
binchicken_co104_28
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
MetaBAT2;2.15;default
broad-scale environmental context
engineered
collection date
2018
completeness score
97.24
completeness software
CheckM2;1.0.2;default
contamination score
0.64
environmental medium
engineered
geographic location (country and/or sea)
Japan
geographic location (latitude)
35.6693
geographic location (longitude)
139.4257
investigation type
metagenome-assembled genome
isolation_source
sludge metagenome
local environmental context
sludge biome
metagenomic source
sludge metagenome
project name
Conductive nano-materials have been reported to accelerate methanogenesis by promoting direct interspecies electron transfer (DIET), while their effects seem to vary depending on operational conditions. The present study examined effects of magnetite nanoparticles (MNPs) on methanogenesis from acetate by soil-derived anaerobic cultures under continuous agitation. We found that MNPs accelerated methanogenesis in agitated cultures as has been observed previously for static cultures. Metabarcoding of 16S rRNA gene amplicons showed that Methanosarcina substantially increased in the presence of MNPs, while DIET-related Geobacter did not occur. Metagenomic and metatranscriptomic analyses confirmed the predominance of Methanosarcina in MPN-supplemented cultures. In addition, genes coding for acetoclastic methanogenesis but not those for hydrogenotrophic methanogenesis were abundantly expressed in the dominant Methanosarcina in the presence of MNPs. These results suggest that MNPs stimulate acetoclastic methanogenesis under continuous agitation.
sample derived from
SAMD00175448,SAMD00175449
sample name
binchicken_co104_28
scientific_name
uncultured Bacteroidales bacterium
sequencing method
Illumina NovaSeq 6000
taxonomic identity marker
multi-marker approach