Metagenome-assembled genome: SRP262948_co1_37
Uncultured Candidatus Berkiella sp.
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR11830853,SRR11830854 of study SRP262948.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
SRP262948_co1_37
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
MetaBAT2;2.15;default
broad-scale environmental context
engineered
collection date
2016-06-21
completeness score
99.32
completeness software
CheckM2;1.0.2;default
contamination score
0.24
environmental medium
engineered
geographic location (country and/or sea)
China
geographic location (latitude)
22.59
geographic location (longitude)
113.97
investigation type
metagenome-assembled genome
isolation_source
activated sludge metagenome
local environmental context
activated sludge biome
metagenomic source
activated sludge metagenome
project name
we used metagenomic assembly and binning methods to decipher and compare the prevalence of ARGs and their potential mobility and hosts in AS reactors treating antibiotic wastewater. To do this, we operated lab-scale sequencing batch reactors of AS process by independently adding five antibiotics of cephalexin, chloramphenicol, kanamycin, sulfamethazine and tetracycline with high concentrations. Specifically, the objectives were (1) to reveal the co-selection effects of different antibiotics for antibiotic resistance by investigating the dynamics of co-existence between ARGs, (2) to evaluate the potential mobility of ARGs by identifying the co-occurrence patterns of ARGs and MGEs, and (3) to explore the host-association of ARGs by recovering large number of metagenome-assembled genomes from AS samples.
sample derived from
SAMN14996806,SAMN14996805
sample name
SRP262948_co1_37
scientific_name
Uncultured Candidatus Berkiella sp.
sequencing method
Illumina HiSeq 4000
taxonomic identity marker
multi-marker approach