Metagenome-assembled genome: ERP117304_co3_188
uncultured Pseudomonadales bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3534967,ERR3534968 of study ERP117304.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
ERP117304_co3_188
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
SemiBin2;2.0.2;default
broad-scale environmental context
engineered
collection date
not provided
completeness score
97.61
completeness software
CheckM2;1.0.2;default
contamination score
0.7
environmental medium
engineered
geographic location (country and/or sea)
not provided
geographic location (latitude)
not provided
geographic location (longitude)
not provided
investigation type
metagenome-assembled genome
isolation_source
biofilm metagenome
local environmental context
biofilm biome
metagenomic source
biofilm metagenome
project name
The establishment of epibacterial communities is fundamental to seaweed health, and fitness, in modulating ecological interactions and may also facilitate adaptation to new environments. Few recent studies have investigated the influence of abiotic factors like light, temperature as drivers of epibacterial community composition on seaweeds. Although salinity can determine bacterial abundance, growth and community composition, influence of salinity as a driver of epibacterial community composition has been rarely investigated for seaweeds and especially under long time scales. We also do not know how abiotic stressors may influence the 'core' bacterial species of seaweeds, if 'core' species exist. Following an initial (immediately after field collection, T0) sampling of epibacterial community of an invasive red seaweed Agarophyton vermicullophylum, we conducted a long term mesocosm experiment for 5 months, to examine the influence of three different salinities (low, medium and high) at two different time points (T1, T2) on the epibacterial community richness and composition of Agarophyton. Metagenomic sequencing showed that epibacterial communities changed significantly according to salinity and time points sampled. Epibacterial richness was significantly different between low and high salinities at both time points. Epibacterial richness also varied significantly among T1 and T2 within low, medium and high salinity level. Irrespective of salinity levels and time points sampled 727 taxa consistently appeared in all Agarophyton samples hinting at the presence of core bacterial species on the surface of the alga. Our results indicate that both salinity and time can be major driving forces in structuring epibacterial communities of seaweeds with respect to richness and β diversity. We highlight the necessity of conducting long term experiments allowing us to detect and understand epibacterial succession over time on seaweeds.
sample derived from
SAMEA5789063,SAMEA5789064
sample name
ERP117304_co3_188
scientific_name
uncultured Pseudomonadales bacterium
sequencing method
Illumina HiSeq 2500
taxonomic identity marker
multi-marker approach