Metagenome-assembled genome: SRP200020_co5_379
uncultured Rhodospirillaceae bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR9166044,SRR9166051 of study SRP200020.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
SRP200020_co5_379
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
SemiBin2;2.0.2;default
broad-scale environmental context
aquatic
collection date
2014-11-01
completeness score
95.43
completeness software
CheckM2;1.0.2;default
contamination score
3.58
environmental medium
aquatic
geographic location (country and/or sea)
Venezuela
geographic location (latitude)
10.617
geographic location (longitude)
-65.167
investigation type
metagenome-assembled genome
isolation_source
seawater metagenome
local environmental context
seawater biome
metagenomic source
seawater metagenome
project name
A persistent chemoautotrophy maximum is present in many anoxic basins at the sulfidic layer's upper boundary, but the microbiology and biogeochemistry that control this feature are poorly understood. In 13 of 31 cruises to the Cariaco Basin the particulate organic carbon pool was enriched in 13C (δ13CPOC as high as -16‰) within the oxic/sulfidic transitional layer compared to photic zone values (-23 to -26‰) and 13C enrichments persisted to depth. During these “heavy” cruises, dissolved O2 and [NO3- + NO2-] concentrations and their computed fluxes to the oxic/sulfidic interface were significantly lower than during “light” cruises. Cruises with isotopically heavy POC were more common between 2013 and 2015 when suspended particles below the photic zone tended to be nitrogen-rich compared to later cruises. Within the chemoautotrophic layer, nitrogen-rich particles (POC/PN< 10) were more likely to be 13C-enriched than nitrogen-poor particles among all cruises, implying these samples were dominated by living cells and fresh detritus rather than laterally transported or extensively decomposed biogenic debris. Dissolved inorganic carbon (DIC) assimilation during “heavy” cruises (n = 3) was faster and occurred deeper than during “light” cruises (n = 2). Metagenomics data from the chemoautotrophic layer during two cruises support prevalence of microorganisms carrying RuBisCO form II genes, which encode a carbon fixation enzyme that discriminates less against heavy isotopes than most other carbon fixation enzymes. Metatranscriptomics data confirm that form II RuBisCO genes were more highly expressed during both cruises and at depths where essential reactants coexist.
sample derived from
SAMN11854482,SAMN11854485
sample name
SRP200020_co5_379
scientific_name
uncultured Rhodospirillaceae bacterium
sequencing method
NextSeq 500
taxonomic identity marker
multi-marker approach