Metagenome-assembled genome: binchicken_co77_125
uncultured Candidatus Saccharibacteria bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR12088346,SRR12088347 of study SRP268746.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
binchicken_co77_125
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
MetaBAT;2.15;default
broad-scale environmental context
aquatic
collection date
2019-07-10
completeness score
94.86
completeness software
CheckM2;1.0.2;default
contamination score
0.06
environmental medium
aquatic
geographic location (country and/or sea)
Spain
geographic location (latitude)
40.546053
geographic location (longitude)
-2.050427
investigation type
metagenome-assembled genome
isolation_source
lake water metagenome
local environmental context
lake water biome
metagenomic source
lake water metagenome
project name
The case study is focused on the crenogenic meromictic Lake El Tobar (Spain), a continental karstified system formed by the sink and solution of a combination of Cretaceous dolomites and Triassic salt-rich Keuper. One of the peculiarities of this system is the formation of a salinity gradient from 0.6 to > 200 mS cm-1, that generates an anoxic saline monimolimnion starting at 13-14 m and reaching the bottom at 18 m of depth. Samples were obtained from 2, 12, 12.5, 13, 13.5, 14, integrated 15.5-16.5 and 18 m of depth to study the microbial vertical zoning and stratification patterns of the lake. Water samples were filtered through a series of 20, 5 and 0.22 micron filters. The 0.22 micron filters were sequenced with high-throughput Shotgun sequencing. We provide 8 metagenomic datasets and MAGs derived from these samples.
sample derived from
SAMN15249361,SAMN15249360
sample name
binchicken_co77_125
scientific_name
uncultured Candidatus Saccharibacteria bacterium
sequencing method
HiSeq X Ten
taxonomic identity marker
multi-marker approach