TADA_B5_OR2_14.2
Drosophila melanogaster
Wildtype Drosophila melanogaster flies were grown in population cages at 25°C. MiMIC fly lines with boundary insertions were grown in large-sized cages at 25°C. 0-16 hour embryo collections were aged at 25°C up to the desired time point (4-20 hours). Two independent collections were performed for each timepoint. The collected embryos were dechorionated using 50% bleach and formaldehyde fixed in 10 ml cross-linking solution (50 mM Hepes, 1 mM EDTA, 0.5 mM EGTA, 100 mM NaCl, 1.8 % formaldehyde, pH 8.0) and 30 ml n-heptane on a shaker table at RT for 15 minutes. The reaction was terminated with 125 mM glycine, 0.1% Triton X-100 in PBS. After washing out the fix, the embryos were blotted dry and frozen in liquid nitrogen.
ENA first public
2025-09-15
INSDC center name
EMBL
INSDC status
public
SUBMISSION_TOOL
ena-upload-cli
SUBMISSION_TOOL_VERSION
0.9.0
Submitter Id
a89de72a-5e86-4cca-a15a-a1b9f779dbfc
age
4-20 hrs
collection date
not provided
common name
fruit fly
genetic modification
insertion
genotype
y[1] w[*];;Mi{y[+mDint2]=B5_OR2-DsRed}MI02296
geographic location (country and/or sea)
not applicable
initial time point
egg laying
sample name
a89de72a-5e86-4cca-a15a-a1b9f779dbfc
scientific_name
Drosophila melanogaster
sex
mixed sex
strain
BDSC 33194