Willamette River at Willamette Park, Feb. 2025
freshwater metagenome
Caveat: the sequencing method produced an abnormal number of chimeras; screening will be especially important with these samples.
isolation_source
river water
collection_date
2025-02-16
geo_loc_name
USA: Oregon, Portland, Willamette River at Willamette Park
lat_lon
45.47575 N 122.667894 W
rel_to_oxygen
aerobe
samp_collect_device
Collected by dipping bottles off of a boat ramp
samp_mat_process
Water samples were returned to the lab at ~ ambient temperature. Duplicate whole-water samples were collected on 0.2 µm pore size, 47 mm diameter filters (Millipore GSWP mixed cellulose ester) using low vacuum pressure from a manual pump. Filters were stored frozen (-20 degC) until DNA was extracted using a ZymoBIOMICS DNA Miniprep Kit. 16S rRNA gene amplicons were generated from combined duplicate samples using primers 515F-Y and 926R. Amplicons were gel-extracted targeting bands at 400-700 bp using a ZymoClean Gel Recovery Kit. Amplicons were sequenced by Azenta US Inc.
samp_size
0.468 and 0.462 L
BioSampleModel
Metagenome or environmental