A007
Mus musculus
Protocols: Lgr5+ISCs were FACS sorted and lyzed in immediately in Trizol. Total RNA from sorted ISCs from dm-aKG or Ctrl treated mice(n=6) were isolated with Trizol. Total RNA was treated with DNase using the Heat&Run gDNA Removal(ArcticZymes) to remove residual DNA From the resulting RNA, RNA-seq libraries were made using the Universal RNA-Seq with NuQuant kit(Tecan). cDNA was generated using a mixture of random and poly(T) priming from 15-16 ng of Total RNA. The cDNA is then fragmented, followed by end repaired, adapter ligation, strand selection and ribodepletion using AnyDeplete mouse probes(Tecan). The final library amplification step was performed with 18x cycles of PCR.
ENA first public
2024-01-01
INSDC center name
Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland Molecular and Integrative Bioscience Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
INSDC status
public
Submitter Id
E-MTAB-15105:A007
age
5
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Lgr5-EGFP-IRES-CreERT2(+/-)
geographic location (country and/or sea)
not collected
individual
PK6337A
isolate
not applicable
organism part
small intestine
sample name
E-MTAB-15105:A007
scientific_name
Mus musculus
sex
female
stimulus
PBS
strain
C57BL/6J