LGM328 bottom seawater sample collected 2023-09-13
seawater metagenome
Environmental DNA full-length 16S rRNA survey sample collected from an estuarine-influenced subtropical coastal system adjacent to the Itajai-Acu estuary, southern Brazil. Sample LGM328 was collected on 2023-09-13 from the bottom layer (approx. 15 m) at a fixed station south of the estuarine mouth.
collection_date
2023-09-13
depth
15 m
env_broad_scale
marine biome
env_local_scale
estuarine-influenced coastal water
env_medium
seawater
geo_loc_name
Brazil: Santa Catarina, Itajai
lat_lon
26.966775 S 48.597608 W
collection_method
Water collected with a 10-L Niskin bottle
isolation_source
Seawater from a fixed estuarine-influenced subtropical coastal station south of the Itajai-Acu estuary
misc_param
stratum: Bottom; season: Winter; sample_id: F_13_Set23; eDNA_concentration_ng_uL: 27.7; DNA_260_280_ratio: 1.9; sequenced_reads: 928; fastq_filename: LGM328_Bottom_13_Sep23.fastq.gz
samp_collect_device
Niskin bottle
samp_mat_process
Sequential vacuum filtration through 0.7 um glass fiber and 0.22 um cellulose acetate filters; 0.22 um filters stored at -20 C until DNA extraction with MagMAX Microbiome Ultra Nucleic Acid Isolation Kit
samp_size
500 mL
samp_store_dur
not collected
samp_store_loc
Laboratorio de Genetica Molecular, UNIVALI, Itajai, Brazil
samp_store_temp
-20 degree Celsius
samp_vol_we_dna_ext
500 mL
size_frac
0.22-0.7 um
size_frac_low
0.22 um
size_frac_up
0.7 um
source_material_id
LGM328
tot_depth_water_col
15 m
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water