INC0669
Homo sapiens
Protocols: INCISE FFPE tissue full sections were cut to be 4μm thick and mounted on glass slides. To generate lysates, the full FFPE tissue was scraped off and placed in 1x FFPE Lysis Buffer (BioSpyder), and deparaffinised. Further processing included incubation in FFPE Protease (BioSpyder), followed by mechanical homogenization by trituration.  Detector oligo hybridization, digestion, and ligation, and subsequent PCR amplification was performed using a unique primer set for each sample, introducing a sample-specific barcode and Illumina adaptors according to manufacturer's instructions.  Barcoded PCR products was added to a flip-spin reservoir and purified using the Macherey-Nagel NucleoSpin Gel and PCR Cleanup Kit (Cat. 740609.50, Macherey-Nagel) and quantified by Qubit using the dsDNA kit (Cat. Q32851, ThermoFisher Scientific), to generate a single library.
ENA first public
2025-07-31
INSDC center name
BAH-GU
INSDC status
public
Submitter Id
E-MTAB-15349:INC0669
age
50+
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
colorectal adenoma
geographic location (country and/or sea)
not collected
individual
INC0669
isolate
not applicable
organism part
colorectum
sample name
E-MTAB-15349:INC0669
scientific_name
Homo sapiens
sex
female
sox9 mutational status (group)
Non-SOX9 Mutant