leaf metagenome
4 surface-sterilized, 1-cm sections of P. menziesii var. menziesii needle lamina tips
collection_date
2018-09-04
env_broad_scale
ENVO:01000211
env_local_scale
ENVO:03600038|ENVO:00000119
env_medium
ENVO:01001121
geo_loc_name
USA:Oregon
host
Pseudotsuga menziesii var. menziesii
lat_lon
44.663325 N 123.688588 W
biol_stat
semi-natural
collection_method
Needles collected on clipped internodes, transferred to plastic bags, kept on ice
growth_habit
erect
host_age
9 years
host_common_name
Coastal Douglas-fir
host_taxid
278161
isolation_source
North side of tree, primary or secondary lateral branch at eye- or shoulder-level, second-year internode, tip of needle lamina
neg_cont_type
empty DNA extraction and empty PCR
plant_sex
monoecious
plant_struc
PO:0025142
pos_cont_type
Pooled DNA extractions of Nothophaeocryptopus gaeumannii and a mixture of Basidiobolus (2 isolates) and Linnemania (1 isolate) extracts
samp_collect_device
Pruning shears
samp_mat_process
Intact needles surface sterilized, removed from internode, sectioned, DNA extracted, fungal ITS2 amplicons generated and sequenced
samp_size
40 sq. mm
source_material_id
b_P03-10-C
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.plant-associated