NHDFs-1
Homo sapiens
Normal human dermal fibroblasts (NHDFs) were isolated from foreskins of young and healthy donors (aged < 10 years) as previously described (Tigges et al. 2013). NHDFs were cultured in Basal Medium Eagle (BME, 41010026, Gibco) supplemented with 10% Fetal Bovine Serum (FBS, Corning), 2 mM L-glutamine (Thermo Fisher Scientific, Waltham, MA, USA), and penicillin-streptomycin (50 U/mL). For UVB-induced senescence,NHDFs at their exponential growth phase were seeded in BME with 1% FBS, 2 mM L-glutamine, and penicillin-streptomycin (50 U/mL). At day three after seeding, cells were exposed to NB-UVB (TL20W/01, Philips) at 500 mJ/cm2 two times a day for five days.
ENA first public
2026-04-25
INSDC center name
URBC
INSDC status
public
Submitter Id
Normal human dermal fibroblasts (NHDFs)-1
cell_type
Primary cell
collected_by
Skin samples were obtained from Clinique St-Luc (Bouge, Belgium) following approval of the Medical Ethical Committee of the Clinique St-Luc and according to the principles set out in the Declaration of Helsinki.
collection date
2023
common name
human
geographic location (country and/or sea)
Belgium
sample name
Normal human dermal fibroblasts (NHDFs)-1
scientific_name
Homo sapiens
tissue_type
Skin