sediment metagenome
Metagenomic raw reads (Illumina) from DGG-T, a longstanding methanogenic toluene-degrading consortium. DNA was extracted from 50 mL of a 20-L DGG-T subculture on September 17th, 2020. At the time of sampling, this subculture was fed weekly with 1.5 mL of neat toluene (~60 mg/L), and degraded toluene at a rate of 11.1-18.6 mg/L/day.
isolation_source
Creosote-contaminated sediments
collection_date
1990
geo_loc_name
USA: Pensacola, Florida
lat_lon
30.40 N 87.23 W
ref_biomaterial
Edwards and Grbić-Galić, 1994, Appl Environ Microbiol 60
rel_to_oxygen
obligate anaerobe
samp_collect_device
Continuous core sampling
samp_mat_process
Samples were obtained from an actively methanogenic sandy zone of the contaminated aquifer, from a depth of approximately 6 m. Aquifer sediments (100 g) were added to sterile, anoxic 250-mL screw cap bottles, topped with 100 mL of sterile, anoxic minimal medium, and amended with 40 µM each of toluene, o-xylene, p-xylene, and ethylbenzene. Upon confirmation that toluene and o-xylene were susceptible to biodegradation, one microcosm was selected to be solely maintained on toluene. This became the parent culture of DGG-T. DGG-T has since been subcultured numerous times and is now maintained in large stainless steel vessels.
samp_size
50 mL
Unique_name
DGG-T_Illumina_metagenome
BioSampleModel
Metagenome or environmental