Metagenome-assembled genome: binchicken_co168_165
uncultured Anaerolineaceae bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3839023,SRR5581382 of study ERP119604.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
binchicken_co168_165
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
MetaBAT;2.15;default
broad-scale environmental context
engineered
collection date
not provided
completeness score
89.9
completeness software
CheckM2;1.0.2;default
contamination score
3.07
environmental medium
engineered
geographic location (country and/or sea)
not applicable
geographic location (latitude)
not provided
geographic location (longitude)
not provided
investigation type
metagenome-assembled genome
isolation_source
activated sludge metagenome
local environmental context
activated sludge biome
metagenomic source
activated sludge metagenome
project name
Bioelectrochemical systems (BES) exploit the interaction between microbes andelectrodes. A field of application thereof, is bioelectrochemical remediation, an effective strategy inenvironments where the absence of suitable electron acceptors limits classic bioremediationapproaches. Understanding the microbial community structure and genetic potential of anodebiofilms is of great interest to interpret the mechanisms occurring in BES. In this study, by using awhole metagenome sequencing approach, taxonomic and functional diversity patterns in theinoculum and on the anodes of three continuous-flow BES for the removal of phenol, toluene andBTEX were obtained. The genus Geobacter was highly enriched on the anodes and tworeconstructed genomes were taxonomically related to the Geobacteraceae family. To functionallycharacterize the microbial community, the genes coding for the anaerobic degradation of toluene,ethylbenzene and phenol were selected as genetic markers for the anaerobic degradation of thepollutants. The genes related with direct extracellular electron transfer (EET) were also analyzed.The inoculum carried the genetic baggage for the degradation of aromatics but lacked the capacityof EET, while anodic bacterial communities were able to pursuit both processes. Themetagenomic approach provided useful insights into the ecology and complex functions withinhydrocarbon degrading electrogenic biofilms.
sample derived from
SAMEA6502094,SAMN07139232
sample name
binchicken_co168_165
scientific_name
uncultured Anaerolineaceae bacterium
sequencing method
Illumina HiSeq 2000,Illumina HiSeq 4000
taxonomic identity marker
multi-marker approach