M0 HMC3_2
Homo sapiens
Protocols: Human microglial clone 3 (HMC3) cells (Cat. No. CRL-3304) and primary renal mixed epithelial cells (hRMECs) (Cat. No. PCS-400-012) were purchased from ATCC. HMC3 cells were treated with 1 μg/mL LPS (Cat. No. HY-D1056, MCE, China) or 20 ng/mL IL-4 (Cat. No. HY-P70445, MCE) for 24 hs. HMC3 and hRMECs were co-cultured using Corning 12 mm Transwell with 0.4 μm pore polycarbonate membrane insert (Cat. No. 3401, Corning, USA) for 48 hs after induction.  Isolated RNA was confirmed RNA purity (A260/A280 ratio should be ~2.0) and integrity (RIN > 7) to ensure high-quality starting material. Use VAHTS mRNA Capture Beads (Vazyme) to capture polyadenylated mRNA, leaving out other RNA types. Convert the enriched or depleted RNA to complementary DNA (cDNA) through reverse transcription. Perform first-strand synthesis using reverse transcriptase and random primers. Follow with second-strand synthesis to create double-stranded cDNA. Perform first-strand synthesis using reverse transcriptase and random primers. Follow with second-strand synthesis to create double-stranded cDNA. Repair the ends of cDNA fragments and add a single \"A\" nucleotide at the 3' end to prevent self-ligation.
ENA first public
2025-06-20
INSDC center name
Department of Ophthalmology, Zibo Center Hospital
INSDC status
public
Submitter Id
E-MTAB-15209:M0 HMC3_2
broker name
ArrayExpress
cell line
HMC3
cell type
microglial cell
collection date
not collected
common name
human
developmental stage
adult
disease
M0
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
brain
sample name
E-MTAB-15209:M0 HMC3_2
scientific_name
Homo sapiens