Metagenome-assembled genome: binchicken_co165_69
uncultured bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR12685295,SRR9952630 of study SRP284337.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
binchicken_co165_69
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
MEGAHIT;1.2.9;default
binning parameters
coverage and kmer
binning software
MetaBAT2;2.15;default
broad-scale environmental context
aquatic
collection date
not provided
completeness score
89.05
completeness software
CheckM2;1.0.2;default
contamination score
4.31
environmental medium
aquatic
geographic location (country and/or sea)
not applicable
geographic location (latitude)
not provided
geographic location (longitude)
not provided
investigation type
metagenome-assembled genome
isolation_source
freshwater sediment metagenome
local environmental context
freshwater sediment biome
metagenomic source
freshwater sediment metagenome
project name
Microbially mediated sulfate reduction in aquatic sediments mineralizes organic carbon, generates hydrogen sulfide, and mediates the geochemical cycles of other elements, such as iron, phosphorus, and mercury. While organic matter contains a number of sulfur compounds, little is known of the fate of this organic sulfur pool during mineralization and more importantly its contribution to the inorganic sulfur cycle that fuels sulfate reduction. The current paradigm of sulfate reduction involves diffusion of sulfur from overlying water into the sediments where sulfur-reducing microorganisms are present. Contrary to the paradigm, modeling and preliminary results demonstrate that under low-sulfate conditions organic sulfur buried in sediment may be the dominant source of sulfur for sulfate reduction, and once mobilized, via microbial biotransformation, may be exported to the overlying water column. Contributions from organic sulfur may be pervasive in environments such as oligotrophic freshwater lakes or the oceans of the geologic past. By characterizing the organic sulfur transformations in sediments across a range of sulfate and organic carbon levels in Lake Superior and its largest American tributary, investigators will address the following questions under a range of environmental conditions: A. To what extent does organic sulfur contribute to the pool of sulfur that fuels sulfate reduction? B. Does organic sulfur undergo cryptic, microbially-mediated biogeochemical transformations, and what microorganisms are responsible for these transformations? They have assembled a multidisciplinary research team that combines expertise in sediment geochemistry, organic geochemistry, and geomicrobiology to address these objectives using sediment characterizations, rate measurements, molecular characterizations of microbial communities, and modeling. The results will quantify an important part of the diagenetic sulfur cycle that has received little attention despite its potential significance in environments such as freshwater lakes, deep subsurface, and the low sulfate oceans of the geological past. Verifying the proposed hypotheses may lead to reevaluation of the geochemical cycles of sulfur and associated elements such as iron and nitrogen, including cryptic reactions in the sulfate-methane transition zone reinterpretation of the origins of the isotopic signatures of sulfur preserved in both modern aquatic sediments and ancient sedimentary rocks and conservation and management practices in sulfide affected water bodies. The project will generate novel microbial and geochemical data that will be publicly available.
sample derived from
SAMN16212730,SAMN12368158
sample name
binchicken_co165_69
scientific_name
uncultured bacterium
sequencing method
Illumina NovaSeq 6000,Illumina HiSeq 2500
taxonomic identity marker
multi-marker approach