H37Rv_1
Mycobacterium tuberculosis H37Rv
Protocols: WT and ΔsubI H37Rv were grown in triplicates in 7H9 medium supplemented with 10% ADC, 0,05% Tyloxapol, 1mM MgSO4, and 1mM cysteine until OD600 =0.8.   Total RNA was extracted from 10 mL of bacterial cultures grown to logarithmic phase (OD600nm = 0.8) using RNeasy kit (Qiagen).  Total Bulk RNA sequencing was performed Each extracted total RNA sample was barcoded with CEL-seq primers during the enzymatic lysis. It was then followed by fragmentation, end repair, poly(A)-tailing, reverse transcription, and second-strand synthesis. After second-strand synthesis, the barcoded samples were pooled into one library and amplified using in vitro transcription. Further, ribosomal aRNA was depleted. After depletion, adapter ligation, reverse transcription, and indexing PCR were performed to prepare a cDNA library.
ENA first public
2025-09-15
INSDC center name
Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS
INSDC status
public
Submitter Id
E-MTAB-15580:H37Rv_1
broker name
ArrayExpress
collection date
not collected
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-15580:H37Rv_1
scientific_name
Mycobacterium tuberculosis H37Rv