Metagenome-assembled genome: binchicken_co179_107
uncultured bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR2586215,ERR2586216,ERR2586217 of study ERP108828.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
binchicken_co179_107
assembly quality
Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
MetaBAT2;2.15;default
broad-scale environmental context
engineered
collection date
2017
completeness score
97.67
completeness software
CheckM2;1.0.2;default
contamination score
0.17
environmental medium
engineered
geographic location (country and/or sea)
Croatia
geographic location (latitude)
45.789725
geographic location (longitude)
16.08232
investigation type
metagenome-assembled genome
isolation_source
metagenome
local environmental context
metagenome
metagenomic source
metagenome
project name
In this work, we have examined sludge samples from a Croatian azithromycin production plant using shotgun metagenomics, to better understand the full diversity of known resistance genes, mobile genetic elements and the impact on taxonomic composition caused by extensive antibiotic selection pressures. We compare this data to sludge from a wastewater treatment plant receiving municipal sewage. We find that the sludge from pharmaceutical production harbors around three times as much resistance genes as the municipal sewage sludge, with particularly large enrichments of aminoglycoside, amphenicol and sulfonamide resistance genes. The findings highlight that antibiotic production does contribute to the development of antibiotic resistance also in European settings and indicate a large potential for co-selection of resistance genes to a variety of antibiotic classes.
sample derived from
SAMEA4668225,SAMEA4668226,SAMEA4668227
sample name
binchicken_co179_107
scientific_name
uncultured bacterium
sequencing method
Illumina HiSeq 2500
taxonomic identity marker
multi-marker approach