Sample1_human
Homo sapiens
Protocols: The cell lines once in suspension were washed with PBS and pelleted by 5 minutes of centrifugation at 400g. The individual suspensions were filtered through a 35um mesh and their densities were adjusted to 106 cells/mL in PBS on ice. An equal volume of each suspension was pooled before being processed at the National Genomics Infrastructure (NGI, Scilifelab, Solna, Sweden). 4T1, A549, MDA-MB-231 clone A, L and S were grown in cell culture medium. Adherent MDA-MB-231 clone L were grown with 1uM of Paclitaxel, a concentration we have previously shown to slow proliferation while retaining a viability compatible with the experiment The cell lines once in suspension were washed with PBS and pelleted by 5 minutes of centrifugation at 400g. The individual suspensions were filtered through a 35um mesh and their densities were adjusted to 106 cells/mL in PBS on ice. An equal volume of each suspension was pooled before being processed at the National Genomics Infrastructure (NGI, Scilifelab, Solna, Sweden) The cell lines once in suspension were washed with PBS and pelleted by 5 minutes of centrifugation at 400g. The individual suspensions were filtered through a 35um mesh and their densities were adjusted to 106 cells/mL in PBS on ice. An equal volume of each suspension was pooled before being processed at the National Genomics Infrastructure (NGI, Scilifelab, Solna, Sweden)
ENA first public
2025-12-05
INSDC center name
Cell and Molecular Biology Department, Karolinska Institutet ScilifeLab
INSDC status
public
Submitter Id
E-MTAB-16354:Sample1_human
broker name
ArrayExpress
cell line
MDAMB231 and A549
cell type
MDAMB231 and A549
collection date
not collected
common name
human
disease
breast cancer and lung cancer
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
breast, lung
sample name
E-MTAB-16354:Sample1_human
scientific_name
Homo sapiens