Metagenome-assembled genome: ERP119294_co4_433
uncultured bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3804377,ERR3804378 of study ERP119294.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
ERP119294_co4_433
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
VAMB;3.0.2;default
broad-scale environmental context
terrestrial
collection date
not provided
completeness score
100
completeness software
CheckM2;1.0.2;default
contamination score
2.2
environmental medium
terrestrial
geographic location (country and/or sea)
not provided
geographic location (latitude)
not provided
geographic location (longitude)
not provided
investigation type
metagenome-assembled genome
isolation_source
sediment metagenome
local environmental context
sediment biome
metagenomic source
sediment metagenome
project name
Lake sediments not only store the long-term ecological information, but are also a source of sedimentary ancient DNA (sedaDNA). Here, by combination of traditional multi-proxy paleolimnological methods with the whole-metagenome shotgun-sequencing of sedaDNA we were able to paint a comprehensive picture of the fluctuations in trophy and microbial diversity and metabolism of a small temperate lake in response to hemp retting, across the past 2000 years. Hemp retting (HR), a key step in hemp fibre production, was historically carried out in freshwater reservoirs and had a negative impact on the lake ecosystems. In Lake Slone, we identified two HR events, during the late stage of Roman and Early Medieval periods, and correlated these to increased trophy and imbalanced lake microbiome. The metagenomic analyses showed a higher abundance of Chloroflexi, Planctomycetes and Bacteroidetes and a functional shift towards anaerobic metabolism, including degradation of complex biopolymers such as pectin and cellulose, during HR episodes. The lake eutrophication during HR was linked to the allochthonous, rather than autochthonous carbon supply – hemp straws. We also showed that the identification of HR based on the palynological analysis of hemp pollen may be inconclusive and we suggest employment of the fibre count analysis as an additional and independent proxy.
sample derived from
SAMEA6464982,SAMEA6464983
sample name
ERP119294_co4_433
scientific_name
uncultured bacterium
sequencing method
NextSeq 500
taxonomic identity marker
multi-marker approach