X3600A_WT
Mus musculus
Protocols: The mice have GFP-tagged PRPF8 on both alleles with the Y2334N mutation for the mutant samples, and no mutation for the control samples. The neural retina was isolated from the mice and immediately frozen in dry ice. Neural retina samples were resuspended in 300 µL of TRIzol and then stored at -80°C. The total RNA was isolated from each sample using the Direct-zol RNA MicroPrep Kit (Cat. No R2062) according to the manufactuerer's instructions. Then, each sample was on-column DNase I treated. Finally, RNA was eluted in 11 µL of DNase/RNase-free water. Ribodepletion and library preparation was done with KAPA RNA HyperPrep Kit with RiboErase KK8560. Then the quality and length distribution was mesured using Agilent 2100 Bioanalyser and Qubit. Pooling was done and primer-dimers were cleaned using Spri-selected beads. A final pool quality and length distribution was measured with Agilent 2100 Bioanalyser and Qubit.
ENA first public
2026-02-19
INSDC center name
IMG
INSDC status
public
Submitter Id
E-MTAB-16570:X3600A_WT
age
8
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Prpf8 WT
geographic location (country and/or sea)
not collected
individual
A
isolate
not applicable
organism part
neural retina
sample name
E-MTAB-16570:X3600A_WT
scientific_name
Mus musculus
sex
male
strain
Prpf8 WT