65 Tcf4 HET
Mus musculus
Protocols: Adult littermates of the Rosa26tdTomato Defa6-iCre mouse strain carrying the Tcf7l2-wt or Tcf4-floxed alleles were sacrificed by cervical dislocation. In these mice, cells expressing Defa6 producing constitutively active Cre recombinase are marked by endogenous expression of the red fluorescent protein tdTomato. The distal part of the small intestinal epithelium was isolated, and a suspension of single cells was prepared by dispase cleavage. Cells were stained with the following antibodies: Hoechst33358, anti-CD45-PacificBlue, anti-CD31-PacificBlue, anti-EpCAM-FITC. Using fluorescence-activated cell sorting (FACS), we isolated Hoechst-/CD45-/CD31-/EpCAM+/tdTomato+ cells, i.e. Defa6+ cells with either wild-type Tcf4 or heterozygous and homozygous loss of Tcf4 protein. Total RNA was isolated using RNeasy Micro Kit (Qiagen) as described in the manufacturer's protocol. Libraries were prepared with a SMARTer® Stranded Total RNA-Seq – Pico Input Mammalian library preparation kit v2 (Takara).
ENA first public
2025-03-26
External Id
SAMEA115129602
INSDC center name
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC last update
2024-01-23T17:34:36Z
INSDC status
public
Submitter Id
E-MTAB-13730:65 Tcf4 HET
age
13
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Rosa26-tdTomato; Tcf7l2-flox/wt; DefaiCre
geographic location (country and/or sea)
not collected
immunophenotype
Hoechst-/CD45-/CD31-/EpCAM+/tdTomato+
individual
J
isolate
not applicable
organism part
epithelium of small intestine
sample name
E-MTAB-13730:65 Tcf4 HET
scientific_name
Mus musculus
sex
female
strain
C57BL/6