Metagenome-assembled genome: SRP272529_co2_240
uncultured Gammaproteobacteria bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR12266071,SRR12266076 of study SRP272529.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
SRP272529_co2_240
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
MetaBAT;2.15;default
broad-scale environmental context
terrestrial
collection date
2019-05-10
completeness score
96.54
completeness software
CheckM2;1.0.2;default
contamination score
2.93
environmental medium
terrestrial
geographic location (country and/or sea)
not provided
geographic location (latitude)
not provided
geographic location (longitude)
not provided
investigation type
metagenome-assembled genome
isolation_source
soil metagenome
local environmental context
soil biome
metagenomic source
soil metagenome
project name
Biodegradable mulch films have been developed as a viable solution to alleviate the growing plastic accumulation problem in soils. However, the key factors controlling the degradation speed of biodegradable mulch films remain obscure. Here, we studied the degradation performance of poly(butylene adipate-co-terephthalate) (PBAT), a promising biodegradable material, in four typical types of soils, including Lou soil (LS), Fluvo-aquic soil (CS), Black soil (BS), and Red soil (RS), through microcosms, and its association with microbiome response. The results showed that the PBAT degradation rate differed with soil type, with PBAT mineralization level of 16%, 9%, 0.3%, and 0.9% in LS, CS, BS, and RS by day 120, respectively. Metagenomic analysis revealed that microbial community of LS was more responsive to PBAT than the other three soils, and the evident degradation of PABT in LS was correlated with the significant increase of PBAT hydrolase genes. Bacteria were the dominant degraders and the enrichment of degraders in PBAT film surface soils determined PBAT degradation capacities. This work advances the understanding of microbiological mechanisms underlying the varying PBAT fate in different types of soils and will therefore have important implications for the development of microbial regulation strategy to improve PBAT biodegradation in soils.
sample derived from
SAMN15580205,SAMN15580201
sample name
SRP272529_co2_240
scientific_name
uncultured Gammaproteobacteria bacterium
sequencing method
Illumina HiSeq 4000
taxonomic identity marker
multi-marker approach