Marinobacter flavimaris
Sample was taken using a sterilized brush attached to a telescopic stick. The brushes were immediately soaked in 15 mL 1 x nine-salt solution (NSS) in 50 mL Falcon tubes. Then, a dilution series 10^-1 to 10^-8 was prepared in 1 x NSS for each sample, and 100 µL was plated out in duplicates on Väätanen nine-salt solution (VNSS) marine agar medium supplemented with 40 µg/mL Congo red and 20 µg/mL Coomassie brilliant blue to aid in identification of different colony morphology. Cycloheximide (40 µg/mL) was added to the agar plates to prevent fungal growth. Ssample set was incubated at 18°C or 24°C for 4–5 days for colony growth.
strain
H11
isolation_source
Marine; submerged hulls of Ophelia vessel
collection_date
2020-10-06
geo_loc_name
Denmark: Helsingor harbor
sample_type
Biofilm
temp
13.5°C
BioSampleModel
Microbe, viral or environmental