6491-3_Fgf2-vs-CBD-CNP_Fgf2_R
Mus musculus
Protocols: Three fresh frozen tibiae per group were OCT embedded, and 10μm frozen sections were cut with cryostat. The tibiae from embryonic day 16.5 mice were cultured in a 24-well plate. The tibiae were divided into two groups (n = 3 per group): one treated with FGF2 alone and the other with FGF2 plus CBD-CNP group. In the FGF2 group, 100 ng/mL FGF2 was administered unilaterally. In the CBD-CNP group, one side received 100 ng/mL FGF2, whereas the opposite site was treated with 100 ng/mL FGF2 and 0.2 µM CBD-CNP for four days with daily medium changes. Nucleic acid was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. Spatial transcriptome sequencing libraries were prepared with the Visium Spatial Gene Expression Slide & Reagent Kit (10x Genomics) according to the manufacturer's instructions.
ENA first public
2025-12-05
INSDC center name
Department of Pediatrics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
INSDC status
public
Submitter Id
E-MTAB-16154:6491-3_Fgf2-vs-CBD-CNP_Fgf2_R
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
embryonic day 16.5
geographic location (country and/or sea)
not collected
individual
Fgf2-vs-CBD-CNP
isolate
not applicable
organism part
right tibia
sample name
E-MTAB-16154:6491-3_Fgf2-vs-CBD-CNP_Fgf2_R
scientific_name
Mus musculus