Temnothorax nylanderi
isolate
Worker
isolation_source
Taken from the wild, kept in lab conditions for 2 weeks before dissection
collection_date
2021-06-29
geo_loc_name
Germany: Lennebergwald Mainz
tissue
Fat body
library_id
F1F
title
RNA-seq of Tenmnothorax nylanderi: fat body
library_strategy
RNA-Seq
library_source
TRANSCRIPTOMIC
library_selection
Oligo-dT
library_layout
paired
platform
ILLUMINA
instrument_model
Illumina NovaSeq 6000
design_description
Anomotaenia brevis infected ant, LPS treated. We isolated the fat body from each worker by removing the gut and trachea from the first gaster segment, leaving only the fat cells, which were placed in a vial containing 50 l Trizol. Samples were frozen on dry ice and subsequently transferred to -80C. RNA was extracted using the Qiagen RNeasy extraction kit. We sent the samples to Novogene (Cambridge, UK) for mRNA library preparation using a NEBNext Ultra RNA Library Prep Kit. The libraries were sequenced on an Illumina Novaseq 6000 in paired-end 150 bp mode, yielding a minimum of 9 Gb per sample.
filetype
fastq
filename
F1F_1.fq
filename2
F1F_2.fq
BioSampleModel
Invertebrate