Parse Evercode WT scRNA-seq — human tumor cell, pLKO ctrl rep 2 (pLKO-T-2)
Homo sapiens
source_name
subcutaneous tumor xenograft (MCF10DCIS.com on aged C57BL/6 mouse breast dECM scaffold, in Rag1-/- host)
tissue
subcutaneous tumor xenograft (MCF10DCIS.com on aged C57BL/6 mouse breast dECM scaffold, in Rag1-/- host)
cell line
MCF10DCIS.com (ATCC CRL-2049) GFP-reporter, pLKO empty-vector control
cell type
tumor cells (CD11b- fraction, human cell line)
genotype
MCF10DCIS.com transduced with pLKO empty-vector (control)
treatment
Cells seeded (50k per scaffold) on a decellularized/delipidized aged C57BL/6 mouse breast dECM, cultured 12 d in DMEM-F12 + 100 U/mL pen-strep + 0.75 ug/mL puromycin, then implanted into a Rag1-/- (B6.129S7-Rag1tm1Mom/J) host (mouse 169072 - M2, right flank behind the fat pad). Tumor harvested at week 7 post-implantation (final tumor volume 361 mm^3). CD11b- tumor-cell fraction.
batch
S1_WT_A1+S2_WT_B1+S3_WT_C1+S4_WT_D1+S5_WT_E1+S6_WT_F1+S7_WT_G1+S8_WT_H1
geo_loc_name
missing
collection_date
missing