E. coli transcript from the AmpD gene (named coli-IVT), randomly chosen from known E. coli open reading frames with >20% Gs and no longer than 1000 bp and with no significant matches with human and mouse genomes, was in vitro transcribed by Trilink Biotechnologies. It consists of 672 bases and includes a polyA tail 120 nt long. The unmodified version was in vitro transcribed with a reaction mix of canonical ribonucleotides (ATP, CTP, GTP, UTP) while modified transcripts were synthesized replacing GTP with ITP (ATP, CTP, UTP, ITP). This construct has a higher inosine density than the pureI sample (~23% vs ~10%) and includes kmers with multiple Is resembling a hyper-edited region.
Escherichia coli
strain
synthetic construct
host
not applicable
collection_date
2024-03-20
geo_loc_name
Italy
sample_type
not applicable
BioSampleModel
Microbe, viral or environmental