Mus musculus
isolate
M1
synthetic
false
sex
female
age
8-12 weeks
age_event
sampling
population
not applicable
ethnicity
not applicable
race
not applicable
strain
C57BL/6
linked_subjects
not applicable
link_type
not applicable
study_group_description
not applicable
disease
not applicable
disease_length
not applicable
disease_stage
not applicable
prior_therapies
not applicable
immunogen
not applicable
treatment
not applicable
medical_history
not applicable
sample_type
organ harvest
tissue
Spleen T cell zone
anatomic_site
not applicable
health_state
healthy
collection_time_point_relative
not applicable
collection_time_point_reference
not applicable
biomaterial_provider
Charles River Laboratories
tissue_processing
Five splenic cryosections (12µm) were lysed in 0.7ml lysis buffer (Analytik Jena)
cell_type
T cell
cell_phenotype
not applicable
single_cell
False
cell_number
On average, 1.8 million sequencing reads were obtained per sample
cells_per_reaction
not applicable
cell_storage
True
cell_quality
not applicable
cell_isolation
not applicable
cell_processing_protocol
Microdissected zones of the spleen were lysed in 700 μl of lysis buffer (Analytik Jena, Hildesheim, Germany). Following RNA isolation (Analytik Jena), TCR β-chain transcripts were amplified independently in a two-step reaction according to the manufacturer's protocol (patent no. 7,999,092; iRepertoire).
collection_date
not applicable
geo_loc_name
not applicable
BioSampleModel
Model organism or animal