UCP1 KO room temperature
Mus musculus
Protocols: Wild-type (WT) and Ucp1-knockout (Ucp1 KO) mice were euthanized at the study endpoint, and metabolic tissues including interscapular brown adipose tissue (iBAT) and inguinal white adipose tissue (iWAT) were excised and collected. For human samples, deep neck adipose tissue biopsies were acquired from 45 individuals. For single-nucleus RNA sequencing, 300-400 mg of fresh or frozen mouse adipose tissue was immediately minced into 1-3 mm pieces on ice for nuclear isolation. For snRNA-seq, mature adipocyte nuclei were isolated by homogenizing minced tissue in 0.1% CHAPS in CST buffer on ice, lysed for 5 minutes, filtered through a 40 μm cell strainer, and purified via FACS sorting based on RFP fluorescence. For RT-qPCR and bulk RNA-seq, total RNA was extracted using Trizol reagent (Invitrogen) followed by DNase (NEB BioLabs) digestion to remove genomic DNA contamination. 10X Genomics libraries for single-nucleus RNA-seq were prepared with the Chromium Single Cell V3.1 reagent kit following the manufacturer's protocol. Nuclei suspensions (~1200 nuclei per μl) were loaded into Chip G followed by reverse transcription to obtain cDNA, which was subsequently amplified and used for library construction. Human tissue bulk RNA-seq libraries were prepared using standard institutional protocols at FGCZ.
ENA first public
2026-06-11
INSDC center name
Institute of Pharmacology and Toxicology, University Hospital Bonn, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany
INSDC status
public
Submitter Id
E-MTAB-17074:UCP1 KO room temperature
age
11
broker name
ArrayExpress
cell type
adipocyte room temperature
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
ucp1 ko room temperature
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
brown adipose tissue
sample name
E-MTAB-17074:UCP1 KO room temperature
scientific_name
Mus musculus
sex
male
strain
UCP1 KO