Sample 4_sublib20
Homo sapiens
Protocols: After d21 in culture 3 organoids per condition per cell line were dissociated individually using CyBio FeliX liquid handler robot with thermoshaker. For dissociation we used a papain-based dissociation kit (Miltenyi Neural dissociation kit). Each organoid was dissociated using 410uL of enzyme mix 1 and 6uL of enzyme mix 2. Then single cell suspension from the same condition were pooled together (H1 and H9; WTC and WIBJ2).  Each individual single cell suspension has been followed by  fixation and  permeabilization procedures, which were performed according to the manufacturer specification (ParseBiosciences, cell fixation kit v3, CF100).  Collected samples were processed for highly multiplexed single-cell RNA sequencing (scRNA-seq) using a split-pool combinatorial barcoding kit (ParseBiosciences, WT Mega kit v3, MG100).
ENA first public
2025-10-02
INSDC center name
ETH Zurich, Department of Biosystems Science and Engineering
INSDC status
public
Submitter Id
E-MTAB-15622:Sample 4_sublib20
broker name
ArrayExpress
cell line
Mixed H1, H9, Wibj2 and WTC
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-15622:Sample 4_sublib20
scientific_name
Homo sapiens
time
21_