SRTP_A_M1_210903_ZMB
Canis lupus familiaris
Dog stool sample of Degesz
ENA first public
2025-02-12
INSDC center name
UNIVERSITY OF SZEGED
INSDC status
public
Submitter Id
Canfam_SRTP_A_M1_210903_ZMB
adapters
5�-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3�
biological status
breeder's line
broad-scale environmental context
Danube Basin
collection date
2021-09-03
common name
dog
environmental medium
no
geographic location (country and/or sea)
Hungary
geographic location (latitude)
47.31955
geographic location (longitude)
19.324268
geographic location (region and locality)
D�ny
gravidity
NA
host age
12
host body product
stool
host common name
Hungarian herding terrier: Pumi
host diet
Gemon adult lamb Rice/Gemon mini starter salmon 3:1
host family relationship
Father of Ilka, Grandfather of Ajsa, Rozi and Mini
host life stage
adult
host scientific name
Canis lupus familiaris
host sex
male
host subject id
SRTP_A_M1
host subspecific genetic lineage
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host taxid
9615
library construction method
Rapid sequencing amplicons - 16S barcoding (SQK-RAB204)
library screening strategy
AMPLICON
library size
1500
local environmental context
Garden
multiplex identifiers
TCGATTCCGTTTGTAGTCGTCTGT
nucleic acid amplification
LongAmp Taq 2X Master Mix
nucleic acid extraction
ZymoBiomics 96 MagBead DNA Kit
pcr conditions
Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
pcr primers
16SFw 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'; 16S Rev 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'
project name
Metagenomic sequencing for longitudinal analysis of dog gut microbiome
sample name
Canfam_SRTP_A_M1_210903_ZMB
sample storage location
Eppendorf_CryoCube_F740h
sample storage temperature
-80
sample volume or weight for DNA extraction
0,1
scientific_name
Canis lupus familiaris
target gene
16S rRNA
target subfragment
V1-V9