PRMT_FP1_FR_PB_20240807_bc40
Homo sapiens
ZymoBIOMICS Fecal Reference isolated by ZymoBIOMICS 96 MagBead DNA Kit
ENA first public
2025-02-12
INSDC center name
UNIVERSITY OF SZEGED
INSDC status
public
Submitter Id
BACMIX_PRMT_FP1_FR_PB_20240807_bc40
adapters
Top strand 5'-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3'; Bottom strand 5'-ACGTAACTGAACGAAGTACAGG-3'
broad-scale environmental context
Csongrad county
collection date
2024-08-04
common name
human
environmental medium
ZymoBIOMICS Fecal Reference
geographic location (country and/or sea)
Hungary
geographic location (latitude)
46.248755
geographic location (longitude)
20.145766
library construction method
16S barcoding (SQK-16S024)/ Native Barcoding Kit 94 V14 (SQK-LSK114.96)
library screening strategy
ligation based
local environmental context
Szeged
multiplex identifiers
TAGTTTGGATGACCAAGGATAGCC/GGCTATCCTTGGTCATCCAAACTA
nucleic acid amplification
LongAmp HotStart Taq 2X Master Mix
nucleic acid extraction
ZymoBIOMICS 96 MagBead DNA Kit
pcr conditions
Initial denaturation 1 min 95 �C (1 cycle); Denaturation 20 secs 95 �C (25 cycles); Annealing 30 secs 55 �C (25 cycles); Extension 2 mins 65 �C (25 cycles); Final extension 5 mins 65 �C (1 cycle)
pcr primers
27F 5'-(AGRGTTYGATYMTGGCTCAG); 1492R 5'-(RGYTACCTTGTTACGACTT)
project name
Evaluating Primer Bias in Full-Length 16S Sequencing of Synthetic and Host Fecal Samples
sample name
BACMIX_PRMT_FP1_FR_PB_20240807_bc40
scientific_name
Homo sapiens
sequence quality check
software
target gene
16S rRNA
target subfragment
V1-V9