4hEV-SB142-143-151
Homo sapiens
Protocols: II trimester human amniotic fluid (hAF) samples were collected as leftover material from prenatal screening. hAF samples were concentrated and processed by size exclusion chromatography (SEC). hAF-EV-enriched fractions were collected and concentrated by ultracentrifugation and then resuspended in a PBS solution. Combined phenol/guanidine-based lysis of samples and silicamembrane–based purification of total RNA. (miRNeasy Micro Kit, Qiagen)  Whole transcriptome RNA-seq analyses have been performed using the Universal RNAseq kit (Nugen, Tecan).
ENA first public
2025-05-01
INSDC center name
Tettamanti Center
INSDC status
public
Submitter Id
E-MTAB-14034:4hEV-SB142-143-151
age
38
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
normal
fraction
Amniotic Fluid Extra Vesicles
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
amniotic fluid
sample name
E-MTAB-14034:4hEV-SB142-143-151
scientific_name
Homo sapiens
sex
female