Sample 3
Mus musculus
Protocols: At the end of the 4-week treatment period, mice were anesthetized. Following euthanasia, the heart tissues were rapidly excised, rinsed in ice-cold phosphate-buffered saline (PBS) to remove residual blood, and then immediately snap-frozen in liquid nitrogen and stored at -80°C until RNA extraction. Total RNA was extracted from the frozen heart tissues using TRIzol reagent (Invitrogen, USA) following the manufacturer's instructions. The concentration and purity of the extracted RNA were determined using a NanoDrop spectrophotometer, and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations. First-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. After end repair and adapter ligation, the products were purified and enriched by PCR to create the final cDNA library.
ENA first public
2026-06-11
INSDC center name
Beijing University of Chinese Medicine Shenzhen Hospital (Longgang)
INSDC status
public
Submitter Id
E-MTAB-17126:Sample 3
broker name
ArrayExpress
collection date
not collected
common name
house mouse
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
heart
sample name
E-MTAB-17126:Sample 3
scientific_name
Mus musculus