NEK9_WT_rep3
Homo sapiens
Protocols: DU145 NEK9 WT and KO cells were cultured in cell culture conditions in DMEM (10%FBS) . Cells were trypsinized and subjected to RNA isolation. Total RNA was isolated using Direct-Zol RNA miniprep (R2052) RNA isolation kit. To prepare an RNA-Sequencing library, 500ng of total RNA was used to isolate mRNA using magnetic beads (PolyA mRNA Isolation Module, NEB Cat No# E7490L). The RNA-Seq library was then prepared using an mRNA library preparation kit (NEB Cat No# E7770L) strictly following the vendor's recommended protocol. Following library preparation, the library concentration was measured using Qubit 2.0 (Invitrogen), and the appropriate fragmentation sizes were confirmed using Bio-analyzer (Agilent Cat No# 5067-5582).
ENA first public
2025-10-02
INSDC center name
Institute of Life Sciences
INSDC status
public
Submitter Id
E-MTAB-14795:NEK9_WT_rep3
broker name
ArrayExpress
cell_line
DU145
cell_type
prostate gland cancer cell
collection date
not collected
common name
human
disease
cancer
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
prostate gland
sample name
E-MTAB-14795:NEK9_WT_rep3
scientific_name
Homo sapiens