Hordeum vulgare
Plants were grown under controlled greenhouse conditions with a 16-hour light/8-hour dark photoperiod at 25 °C during the day and 18 °C at night. Humidity was maintained at 60%, and plants were irrigated weekly with a nutrient solution. Leaf tissue samples were collected from young plants (2-3 weeks old) for DNA extraction. High molecular weight (HMW) DNA was extracted from a single Asplund plant using the MACHEREY-NAGEL NucleoBond HMW DNA kit, following the manufacturer¿s protocol. DNA concentration was assessed using the Qubit 2.0® dsDNA HS assay (Invitrogen, Waltham, USA), and fragment size distribution was evaluated with the Agilent Femto Pulse System (Agilent Technologies, Santa Clara, USA). For HiFi sequencing, the HMW DNA was sheared into ~20 kb fragments using a Megaruptor device (Diagenode) at speed setting 30. HiFi SMRTbell libraries were prepared using the Pacific Biosciences SMRTbell Express Template Prep Kit 3.0. Final libraries were size-selected to a narrow 17-20 kb range using the SageELF system with a 0.75% agarose gel cassette (Sage Science), in accordance with the manufacturer¿s instructions. HiFi circular consensus sequencing (CCS) reads were generated on the PacBio Revio platform (Pacific Biosciences) following standard operating procedures (https://github.com/pacificbiosciences).
IPK sequencing date
2025-03-19
accession name
not collected
age
2-3 weeks
air temperature regimen
25°C day/18°C night
biological material DOI
doi:10.25642/IPK/GBIS/7828045
biological material ID
IPK:HOR 2805 BRG
biological material altitude
112m
biological material coordinates uncertainty
2000m
biological material geographic location
Germany
biological material latitude
51.816
biological material longitude
11.283
biological material ploidy
diploid
biological material preprocessing
not applicable
checklist
ERC000011
collection date
not collected
genus
Hordeum
geographic location (country and/or sea)
Iran
infraspecific name
convar. vulgare var. hybernum
light regimen
16/8-hour light
material source DOI
doi:10.25642/IPK/GBIS/18832
material source ID
IPK:HOR 2805
material source altitude
1800
material source description
Shamss Abad, 114 km von Esfahan, Weg nach Natanz
material source geographic location
Iran
material source latitude
33.566667
material source longitude
51.866667
plant anatomical entity
leaf
plant structure development stage
seedling
sample ID
IPK_LIMS:3389187
sample name
SHAPE3_HiFi_439_HOR_2805_BRG_ass
species
vulgare