MCF7_shNR2F6_C_REP1
Homo sapiens
Protocols: Before library preparation, RNA concentration was evaluated through Qubit™ RNA High Sensitivity Assay Kit (Invitrogen, Walthman, MA, USA) while RNA quality was established using 4200 Tapestation (Agilent Technologies, Santa Clara, CA, USA).  Protocol: miRNeasy Mini Kit. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing. TruSeq Stranded Total RNA Sample Preparation Guide. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing.
ENA first public
2025-04-15
INSDC center name
Istituto di Ricerche Farmacologiche \"Mario Negri\" IRCCS
INSDC status
public
Submitter Id
E-MTAB-14498:MCF7_shNR2F6_C_REP1
broker name
ArrayExpress
cell_line
MCF7
cell_type
breast invasive ductal carcinoma cell
collection date
not collected
common name
human
disease
breast cancer
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
breast
sample name
E-MTAB-14498:MCF7_shNR2F6_C_REP1
scientific_name
Homo sapiens