TAC-K3_S25
Rattus norvegicus
Protocols: Left ventricular myocardial samples were collected from rat hearts, snap-frozen in liquid nitrogen immediately after excision, and stored at −80°C until RNA extraction. Total RNA was isolated from rat heart tissue using a chloroform/isopropanol precipitation method. Samples were homogenized in QIAzol® reagent (Qiagen, Venlo, The Netherlands) with TissueLyser (Qiagen, Venlo, The Netherlands), followed by chloroform phase separation and isopropanol precipitation of total RNA. RNA pellets were washed with 75% ethanol and resuspended in nuclease-free water. For mRNA-Seq library construction, NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 with Poly(A) Beads 2.0 (PerkinElmer, Waltham, MA, USA) was applied according to the manufacturer's protocol. The quality and quantity of the library was determined by using High Sensitivity DNA1000 ScreenTape system with 2200 Tapestation (Agilent Technologies, Santa Clara, CA, USA) and dsDNA HS Assay Kit with Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).
ENA first public
2026-06-11
INSDC center name
Heart and Vascular Center, Semmelweis University
INSDC status
public
Submitter Id
E-MTAB-17114:TAC-K3_S25
age
20
broker name
ArrayExpress
collection date
not collected
common name
Norway rat
developmental stage
adult
disease
heart failure
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
TAC-K3
injury
transverse aortic constriction
isolate
not applicable
organism part
heart left ventricle
sample name
E-MTAB-17114:TAC-K3_S25
scientific_name
Rattus norvegicus
sex
male
strain
Wistar