Sample 5- PFF+S62
Homo sapiens
Protocols: Human fetal cortical neurons were cultured under standard conditions and treated with alpha-synuclein preformed fibrils (PFFs) and/or peptide inhibitors as indicated. At defined time points, cells were collected and dissociated into single-cell suspensions using TrypLE (Thermo Fisher Scientific, 12605010) according to the manufacturer's instructions. Cells were washed and resuspended for downstream single-cell library preparation. RNA extraction was not performed separately. Instead, single-cell suspensions were directly processed for single-cell RNA-seq library preparation, where cell lysis and RNA capture were performed within the workflow according to the manufacturer's protocol. Single-cell RNA-seq libraries were prepared using a droplet-based platform (10x Genomics Chromium) following the manufacturer's protocol. Briefly, single cells were encapsulated with barcoded beads, and reverse transcription was performed to generate cDNA. Amplified cDNA was used for library construction, including fragmentation, end repair, adapter ligation, and PCR amplification.
ENA first public
2026-04-10
INSDC center name
epartment of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom
INSDC status
public
Submitter Id
E-MTAB-16863:Sample 5- PFF+S62
broker name
ArrayExpress
cell type
neuron
collection date
not collected
common name
human
disease
Parkinson's disease
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
brain
sample name
E-MTAB-16863:Sample 5- PFF+S62
scientific_name
Homo sapiens