Sample 1
Homo sapiens
Protocols: Apatinib sensitive and resistant primary HUVECs were cultured in 2 μmol/L apatinib for 48 h, and collected for RNA isolation. Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) Sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina, and library quality was assessed using the Agilent 2100 Bioanalyzer System (Agilent Technologies).
ENA first public
2025-10-30
INSDC center name
The Second Hospital & Clinical Medical School, Lanzhou University
INSDC status
public
Submitter Id
E-MTAB-15692:Sample 1
broker name
ArrayExpress
cell line
HUVEC cell line
cell type
Resistant
collection date
not collected
common name
human
developmental stage
adult
disease
cancer
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
vasculature
sample name
E-MTAB-15692:Sample 1
scientific_name
Homo sapiens