GEX1
Mus musculus
Protocols: Tumor-derived single-cell suspensions were prepared as previously described. After preparation, living single cells were sorted, and a minimum of 500,000 viable cells per sample were subjected to further CITE-seq processing. No separate nucleic acid extraction protocol is described in the provided text. Cells were processed directly as viable single-cell suspensions for 10x Genomics single-cell library preparation following live cell sorting. After live single-cell sorting, at least 500,000 cells were incubated with the TotalSeq™-B Mouse Universal Cocktail V1.0 according to the manufacturer's protocol for 30 minutes on ice. Cells were then washed three times with FACS buffer and resuspended in 100 µl PBS containing 2% FBS. CITE-seq libraries were prepared using the Chromium Next GEM Single Cell 3' Reagent Kit v3.1 with Dual Indexing. For each sample, one gene expression library and one cell surface protein plus hashtag oligo library were generated.
ENA first public
2026-06-11
INSDC center name
Postdoc at German Cancer Research Center
INSDC status
public
Submitter Id
E-MTAB-17099:GEX1
age
>6
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
GEX
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Tumor
sample name
E-MTAB-17099:GEX1
scientific_name
Mus musculus
sex
unknown
strain
C57BL/6