PRMT_CP2_canis_PB_20240807_bc32
Canis lupus familiaris
Dog stool sample
ENA first public
2025-02-12
INSDC center name
UNIVERSITY OF SZEGED
INSDC status
public
Submitter Id
Canfam_PRMT_CP2_canis_PB
broad-scale environmental context
Csongrad-Csanad county
collection date
2023-03-25
common name
dog
environmental medium
no
geographic location (country and/or sea)
Hungary
geographic location (latitude)
46.248755
geographic location (longitude)
20.145766
host age
2.8
host sex
female
library construction method
16S barcoding (SQK-16S024)/ Native Barcoding Kit 94 V14 (SQK-LSK114.96)
local environmental context
Szeged
multiplex identifiers
CCAGTAGAAGTCCGACAACGTCAT/ATGACGTTGTCGGACTTCTACTGG
nucleic acid extraction
ZymoBIOMICS 96 MagBead DNA Kit
pcr primers
27F 5'-(AGRGTTYGATYMTGGCTCAG); 1492R 5'-(RGYTACCTTGTTACGACTT)
project name
Evaluating Primer Bias in Full-Length 16S Sequencing of Synthetic and Host Fecal Samples
sample name
Canfam_PRMT_CP2_canis_PB
sample storage duration
501
sample storage temperature
-80
scientific_name
Canis lupus familiaris
sequence quality check
software
sequencing method
Oxford Nanopore
target gene
16S rRNA
target subfragment
V1-V9
temperature
11