CarSAV_infected
Dianthus caryophyllus
Protocols: Samples were taken from CarSAV infected as well as from healthy culture for total RNA extraction.  Carnation (D. caryophyllus L. var. Tanga) plantlets were micropropagated and cultured based on the method Piquares and co-workers (2002). The extraction of small RNA and small RNA enrichment was carried out based on the method Cárdenas et al. (2011). For miRNA-Seq library construction, NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA, USA) was used according to the manufacturer's protocol. The quality and quantity of the library were determined by using a High Sensitivity DNA1000 ScreenTape system with 2200 Tapestation (Agilent Technologies, Santa Clara, CA, USA) and dsDNA HS Assay Kit with Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).
ENA first public
2025-05-01
INSDC center name
Agricultural Institute, HUN-REN Centre for Agricultural Research
INSDC status
public
Submitter Id
E-MTAB-14576:CarSAV_infected
broker name
ArrayExpress
collection date
not collected
common name
clove pink
cultivar
Tanga
geographic location (country and/or sea)
not collected
infect
CarSAV infection
isolate
not applicable
organism part
portion of plant tissue
sample name
E-MTAB-14576:CarSAV_infected
scientific_name
Dianthus caryophyllus