SRTP_A_F1_210915_ZMB
Canis lupus familiaris
Dog stool sample of Ilka
ENA first public
2025-02-12
INSDC center name
UNIVERSITY OF SZEGED
INSDC status
public
Submitter Id
Canfam_SRTP_A_F1_210915_ZMB
adapters
5�-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3�
biological status
breeder's line
broad-scale environmental context
Danube Basin
collection date
2021-09-15
common name
dog
environmental medium
no
geographic location (country and/or sea)
Hungary
geographic location (latitude)
47.31955
geographic location (longitude)
19.324268
geographic location (region and locality)
D�ny
gravidity
Non Gravid
host age
8
host body product
stool
host common name
Hungarian herding terrier: Pumi
host diet
Gemon adult lamb Rice/Gemon mini starter salmon 3:1
host family relationship
Daughter of Degesz
host life stage
adult
host scientific name
Canis lupus familiaris
host sex
female
host subject id
SRTP_A_F1
host subspecific genetic lineage
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host taxid
9615
library construction method
Rapid sequencing amplicons - 16S barcoding (SQK-16S024)
library screening strategy
AMPLICON
library size
1500
local environmental context
Garden/House
multiplex identifiers
TTCAGGGAACAAACCAAGTTACGT
nucleic acid amplification
LongAmp Taq 2X Master Mix
nucleic acid extraction
ZymoBiomics 96 MagBead DNA Kit
pcr conditions
Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
pcr primers
16SFw 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'; 16S Rev 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'
project name
Metagenomic sequencing for longitudinal analysis of dog gut microbiome
sample name
Canfam_SRTP_A_F1_210915_ZMB
sample storage location
Eppendorf_CryoCube_F740h
sample storage temperature
-80
sample volume or weight for DNA extraction
0,1
scientific_name
Canis lupus familiaris
target gene
16S rRNA
target subfragment
V1-V9