DF_ctl_2
Homo sapiens
Protocols: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology, Livermore CA). The rotation of the cone captures healthy unidirectional flow (UF) seen in athero-protective distal internal carotid artery, while the disturbed flow (DF) mimics athero-susceptible human carotid artery. During flow application to cells, the flow device was placed at 37C with 5%CO2. TeloHAECs subjected to UF and DF were lysed and RNA was extracted as mentioned below. Two technical replicates for each condition were pooled together to obtain an experimental replicate. TeloHAECs are grown in EGM-2 growth media (Lonza).  TeloHAECs subjected to UF and DF were lysed, and total RNA was collected using a NucleoSpin RNA kit (Macherey-Nagel, #740955). Using a TruSEQ mRNA RNA-SEQ library preparation protocol (Illumina provided), strand-specific RNA-SEQ libraries were prepared.
ENA first public
2026-04-10
INSDC center name
University of Chicago National University of Singapore
INSDC status
public
Submitter Id
E-MTAB-16095:DF_ctl_2
broker name
ArrayExpress
cell line
TeloHAEC
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-16095:DF_ctl_2
scientific_name
Homo sapiens
stimulus
DF_ctl_2