Hordeum vulgare
Plants were grown under controlled greenhouse conditions with a 16-hour light/8-hour dark photoperiod at 25 °C during the day and 18 °C at night. Humidity was maintained at 60%, and plants were irrigated weekly with a nutrient solution. Leaf tissue samples were collected from young plants (2-3 weeks old) for DNA extraction. High molecular weight (HMW) DNA was extracted from a single Asplund plant using the MACHEREY-NAGEL NucleoBond HMW DNA kit, following the manufacturer¿s protocol. DNA concentration was assessed using the Qubit 2.0® dsDNA HS assay (Invitrogen, Waltham, USA), and fragment size distribution was evaluated with the Agilent Femto Pulse System (Agilent Technologies, Santa Clara, USA). For HiFi sequencing, the HMW DNA was sheared into ~20 kb fragments using a Megaruptor device (Diagenode) at speed setting 30. HiFi SMRTbell libraries were prepared using the Pacific Biosciences SMRTbell Express Template Prep Kit 3.0. Final libraries were size-selected to a narrow 17-20 kb range using the SageELF system with a 0.75% agarose gel cassette (Sage Science), in accordance with the manufacturer¿s instructions. HiFi circular consensus sequencing (CCS) reads were generated on the PacBio Revio platform (Pacific Biosciences) following standard operating procedures (https://github.com/pacificbiosciences).
IPK sequencing date
2025-03-11
accession name
not collected
age
2-3 weeks
air temperature regimen
25°C day/18°C night
biological material DOI
doi:10.25642/IPK/GBIS/7829670
biological material ID
IPK:HOR 11419 BRG
biological material altitude
112m
biological material coordinates uncertainty
2000m
biological material geographic location
Germany
biological material latitude
51.816
biological material longitude
11.283
biological material ploidy
diploid
biological material preprocessing
not applicable
checklist
ERC000011
collection date
not collected
genus
Hordeum
geographic location (country and/or sea)
India
infraspecific name
convar. vulgare var. coeleste
light regimen
16/8-hour light
material source DOI
doi:10.25642/IPK/GBIS/56460
material source ID
IPK:HOR 11419
material source altitude
not collected
material source description
Uttar Pradesh, Sankola, Kumaon
material source geographic location
India
material source latitude
not collected
material source longitude
not collected
plant anatomical entity
leaf
plant structure development stage
seedling
sample ID
IPK_LIMS:3387247
sample name
SHAPE3_HiFi_429_HOR_11419_BRG_ass
species
vulgare