VBPNA 3
Mus musculus
Protocols: Tumors were collected by surgical excision from the colon or small intestine of mice. RNA was extracted from tumor and organoid samples using the RNeasy Mini Kit following the manufacturer's protocol (Qiagen, #74106). RNA quantity and integrity were evaluated on an Agilent Bioanalyzer using the Agilent RNA Kit (#5067-1513). Whole-transcriptome sequencing libraries were prepared using a modified SMART-seq2-based workflow. Reverse transcription was carried out using 1 µM template-switching oligo (IDT), 1 mM dithiothreitol (Takara Bio Clontech, #707265ML), 1 U/µl RNase inhibitor (Promega, #N2615), Maxima H Minus Reverse Transcriptase (Thermo Fisher/Life Technologies, #EP0753), and 1× Maxima H Minus Reverse Transcriptase buffer. The resulting cDNA was tagmented and further processed for sequencing library generation using the Nextera XT DNA Library Preparation Kit (Illumina, #FC-121-1030).
ENA first public
2026-06-11
INSDC center name
Postdoc at DKFZ
INSDC status
public
Submitter Id
E-MTAB-17076:VBPNA 3
age
minimum 8 weeks
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
VBPNA
geographic location (country and/or sea)
not collected
individual
VBPNA3
isolate
not applicable
organism part
Tumor
sample name
E-MTAB-17076:VBPNA 3
scientific_name
Mus musculus
sex
unknown
strain
C57BL/6