511418_Sod2_ko
Mus musculus
Protocols: Cells were seeded at a density of 10.000 cells in 6 cm dishes and after attachment, were cultured for 24 hours. Culture in standard medium +/- 60 µM 10058-F4 (Myc Inhibitor) Total RNA of cancer cell lines was isolated using Maxwell® 16 LEV simplyRNA Tissue Kit (AS1280, Promega) and Maxwell® 16 Instrument (Promega) as per manufacturer's instructions Library preparation for bulk 3'-sequencing of poly(A)-RNA was done as described previously (Parekh et al., 2016) barcoded cDNA of each sample was generated with a Maxima RT polymerase (Thermo Fisher) using oligo-dT primer containing barcodes, unique molecular identifiers (UMIs) and an adapter. 5' ends of the cDNAs were extended by a template switch oligo (TSO) and after pooling of all samples full-length cDNA was amplified with primers binding to the TSO-site and the adapter. cDNA was tagmented with the Nextera XT kit (Illumina) and 3'-end-fragments finally amplified using primers with Illumina P5 and P7 overhangs.
ENA first public
2025-12-23
INSDC center name
Klinik und Poliklinik für Innere Medizin II TUM Klinikum rechts der Isar Universität München
INSDC status
public
Submitter Id
E-MTAB-16320:511418_Sod2_ko
broker name
ArrayExpress
cell line
cancer cell line
collection date
not collected
common name
house mouse
disease
pancreatic cancer
genotype
KrasG12D; ∆Sod2
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
pancreas
sample name
E-MTAB-16320:511418_Sod2_ko
scientific_name
Mus musculus