SZ25TE_CNT
Homo sapiens
Protocols: For single-cell transcriptomic analysis, stromal and epithelial cells from control and hormone-treated ADOC were collected separately. Stromal cells were first detached by incubation with TrypLE Select® for 15 min and recovered in stromal medium. Subsequently, epithelial cells were dissociated by a 30 min incubation with TrypLE Select® and recovered in organoid medium. Both cell fractions were centrifuged at 300 × g for 5 min at room temperature, the supernatant discarded, and the pellet resuspended in PBS supplemented with 0.04% BSA. Cell concentration and viability were determined using a LUNA™-FL dual fluorescence cell counter (Logos Biosystems) with acridine orange/ propidium Iodide staining (Logos Biosystems, F23001).   Approximately 17,000 cells (combined stromal and epithelial fractions) were loaded onto 10X Chromium G Chip (10X Genomics, PN-10000204) to generate Gel Bead-in-Emulsions (GEMs). Barcoded cDNA libraries were prepared following the manufacturer's instructions using the Chromium Single Cell 3′ Reagent Kit v3.1 (10X Genomics, PN-1000268). Amplified cDNA was quantified with the TapeStation D5000 (Agilent, 5067-5589). Gene expression libraries were constructed with 100-300 ng of amplified cDNA, and library size and concentration were assessed using the TapeStation D5000 kit.
individual
25
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
female
scientific_name
Homo sapiens
cell type
stromal cells and epithelial cells
common name
human
organism part
endometrium
isolate
not applicable
condition
CNT
geographic location (country and/or sea)
not collected