LT3_HF_AL_2
Mus musculus
Protocols: Perigonadal white adipose tissue was dissected from mice after cervical dislocation while under isoflurane euthanasia. Tissue was immediately frozen in liquid nitrogen after dissection Adipose samples were lysed using TRIzol reagent. Total RNA was extracted using the Direct-zol RNA MiniPrep kit (Zymo Research) cDNA libraries were generated using the Nugen Ovation SPIA chemistry with a fully automated Wafergenâ€™s Apollo liquid handler
strain
C57/BL6
individual
16 week Ad libitum Mouse 2
organism
Mus musculus
organism
Mus musculus
collection date
not collected
sex
male
scientific_name
Mus musculus
common name
house mouse
organism part
perigonadal white adipose tissue
developmental stage
adult
genotype
wild type genotype
isolate
not applicable
diet
45% High Fat Diet
age
23
geographic location (country and/or sea)
not collected