Greudnitz 10_113_R3S3 mtCOI metabarcoding from multiplexed PCR, Qiagen kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
QIA
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-10-R-TAIL-3
SampleName
113
Submitter Id
F-HEAD-10-R-TAIL-3.QIA.COI_lg
TagFWD
GACGATG
TagREV
GTCGCCA
adapters
FWD: GACGATG REV: GTCGCCA
broad-scale environmental context
ENVO:01000245
collection date
2020-10-07
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
51.68981629
geographic location (longitude)
12.82736103
lat
51.68981629018775
local environmental context
nest tube of cavity-nesting hymenoptera
lon
12.827361028695893
multiplex identifiers
FWD: GACGATG REV: GTCGCCA
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
project name
PilotNA
removal_date
2020-10-07
sample name
F-HEAD-10-R-TAIL-3.QIA.COI_lg
scientific_name
insect work metagenome
site
Greudnitz
site_ID
GR
source material identifiers
10_113_R3S3
target gene
mtCOI
target subfragment
long barcoding region
trap_ID
10_113_R3S3