Jemmeritz 1_003_R2S3 ITS2 metabarcoding from multiplexed PCR, Qiagen kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
QIA
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-12-R-TAIL-1
SampleName
003
Submitter Id
F-HEAD-12-R-TAIL-1.QIA.ITS2_lg
TagFWD
CCCCAAG
TagREV
TCTACTG
adapters
FWD: CCCCAAG REV: TCTACTG
broad-scale environmental context
ENVO:01000245
collection date
2020-10-09
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
52.64712001
geographic location (longitude)
11.24680600
lat
52.64712000968503
local environmental context
nest tube of cavity-nesting hymenoptera
lon
11.246806000626039
multiplex identifiers
FWD: CCCCAAG REV: TCTACTG
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
project name
PilotNA
removal_date
2020-10-09
sample name
F-HEAD-12-R-TAIL-1.QIA.ITS2_lg
scientific_name
insect work metagenome
site
Jemmeritz
site_ID
JE
source material identifiers
1_003_R2S3
target gene
ITS2
target subfragment
barcoding region
trap_ID
1_003_R2S3