Jemmeritz 1_006_R1S1 mtCOI metabarcoding from multiplexed PCR, NEB kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
NEB
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-24-R-TAIL-3
SampleName
006
Submitter Id
F-HEAD-24-R-TAIL-3.NEB.COI_lg
TagFWD
CCAGTGC
TagREV
GTCGCCA
adapters
FWD: CCAGTGC REV: GTCGCCA
broad-scale environmental context
ENVO:01000245
collection date
2020-10-09
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
52.63508101
geographic location (longitude)
11.23317200
lat
52.6350810092732
local environmental context
nest tube of cavity-nesting hymenoptera
lon
11.233172000596811
multiplex identifiers
FWD: CCAGTGC REV: GTCGCCA
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
project name
PilotNA
removal_date
2020-10-09
sample name
F-HEAD-24-R-TAIL-3.NEB.COI_lg
scientific_name
insect work metagenome
site
Jemmeritz
site_ID
JE
source material identifiers
1_006_R1S1
target gene
mtCOI
target subfragment
long barcoding region
trap_ID
1_006_R1S1