Badetz 5_052_R2S2 ITS2 metabarcoding from multiplexed PCR, NEB kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
NEB
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-15-R-TAIL-3
SampleName
052
Submitter Id
F-HEAD-15-R-TAIL-3.NEB.ITS2_lg
TagFWD
AGCCCAA
TagREV
GTCGCCA
adapters
FWD: AGCCCAA REV: GTCGCCA
broad-scale environmental context
ENVO:01000245
collection date
2020-10-06
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
51.93669824
geographic location (longitude)
11.99721070
lat
51.93669823975997
local environmental context
nest tube of cavity-nesting hymenoptera
lon
11.997210701655606
multiplex identifiers
FWD: AGCCCAA REV: GTCGCCA
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
project name
PilotNA
removal_date
2020-10-06
sample name
F-HEAD-15-R-TAIL-3.NEB.ITS2_lg
scientific_name
insect work metagenome
site
Badetz
site_ID
BAD
source material identifiers
5_052_R2S2
target gene
ITS2
target subfragment
barcoding region
trap_ID
5_052_R2S2