Kalitz 3_032_R2S1 mtCOI metabarcoding from multiplexed PCR, Qiagen kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
QIA
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-7-R-TAIL-4
SampleName
032
Submitter Id
F-HEAD-7-R-TAIL-4.QIA.COI_lg
TagFWD
CCAGATA
TagREV
CTGCGAG
adapters
FWD: CCAGATA REV: CTGCGAG
broad-scale environmental context
ENVO:01000245
collection date
2020-10-05
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
52.08514307
geographic location (longitude)
12.00275901
lat
52.085143065339516
local environmental context
nest tube of cavity-nesting hymenoptera
lon
12.00275900521159
multiplex identifiers
FWD: CCAGATA REV: CTGCGAG
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
project name
PilotNA
removal_date
2020-10-05
sample name
F-HEAD-7-R-TAIL-4.QIA.COI_lg
scientific_name
insect work metagenome
site
Kalitz
site_ID
KA
source material identifiers
3_032_R2S1
target gene
mtCOI
target subfragment
long barcoding region
trap_ID
3_032_R2S1