SW-SL-AN-01
soil metagenome
ENA first public
2025-03-31
INSDC center name
Scotland's Rural College - Aberdeen Campus
INSDC status
public
Submitter Id
ms00480
adapters
Forward overhang: 5'-AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT; Reverse overhang: 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATGACTATCTCGTATGCCGTCTTCTGCTTG
broad-scale environmental context
environmental system determined by an organism
chimera check software
N/A
climate environment
controlled glasshouse
collection date
2021-12-30
environmental medium
rhizosphere environment
experimental factor
Wheat Rhizosphere
geographic location (country and/or sea)
United Kingdom
geographic location (latitude)
51.8094
geographic location (longitude)
0.3561
host age
3
host common name
spring wheat
host life stage
flowering
host subspecific genetic lineage
Mulika
host taxid
4565
local environmental context
plant-associated environment
multiplex identifiers
NA
negative control type
N/A
nucleic acid amplification
https://www.neb.com/en-gb/protocols/2014/05/22/protocol-for-use-with-nebnext-ultra-dna-library-prep-kit-for-illumina-e7370
nucleic acid extraction
Qiagen(TM) Power soil gDNA extraction kit
pcr conditions
98�C for 30 seconds; 10 cycles of 98�C for 10 seconds, 98�C for 75 seconds; 98�C for 5 minutes; Hold at 4�C
pcr primers
NA
plant body site
root
plant product
N/A
positive control type
synthetic community of three known bacterial species
project name
UK Crop Microbiome Cryobank
reference for biomaterial
Rothamsted Research Station
relevant electronic resources
agmicrobiomebase.org
sample name
ms00480
sample volume or weight for DNA extraction
0.5
scientific_name
soil metagenome
sequence quality check
software
sequencing method
Novaseq 6000
target gene
NA