Steuden 8_085_R4S2 mtCOI metabarcoding from multiplexed PCR, NEB kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
NEB
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-16-R-TAIL-1
SampleName
085
Submitter Id
F-HEAD-16-R-TAIL-1.NEB.COI_lg
TagFWD
TGGTTAG
TagREV
TCTACTG
adapters
FWD: TGGTTAG REV: TCTACTG
broad-scale environmental context
ENVO:01000245
collection date
2020-10-08
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
51.41267004
geographic location (longitude)
11.78742400
lat
51.412670041958904
local environmental context
nest tube of cavity-nesting hymenoptera
lon
11.787424003002695
multiplex identifiers
FWD: TGGTTAG REV: TCTACTG
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
project name
PilotNA
removal_date
2020-10-08
sample name
F-HEAD-16-R-TAIL-1.NEB.COI_lg
scientific_name
insect work metagenome
site
Steuden
site_ID
ST
source material identifiers
8_085_R4S2
target gene
mtCOI
target subfragment
long barcoding region
trap_ID
8_085_R4S2