Secale cereale
ENA first public
2026-03-11
INSDC status
public
accession_name
not collected
age
2 weeks
air temperature regimen
26°C day/21°C night
biological material ID
Lo7_rep1
biological material altitude
112m
biological material coordinates uncertainty
2000m
biological material geographic location
Germany
biological material latitude
51.816
biological material longitude
11.283
biological material preprocessing
not applicable
biological_material_doi
not collected
biological_material_id
Lo7_rep1
biological_material_ploidy
diploid
checklist
ERC000011
collection date
not collected
genus
Secale
geographic location (country and/or sea)
Germany
growth facility
growth chamber
infraspecific_name
not collected
ipk_sequencing_date
2026-01-14
light regimen
16/8-hour light
material source geographic location
Germany
material_source_altitude
not collected
material_source_description
not collected
material_source_doi
not collected
material_source_geographic_location
not collected
material_source_id
not collected
material_source_latitude
not collected
material_source_longitude
not collected
plant structure development stage
seedling development stage
plant_anatomical_entity
leaf
sample name
Lo7_rep1
sample_description
The sample material for molecular analysis consisted of 2-week-old leaves from an individual Lo7 plant. All the plants were grown in soil in a growth chamber at 26 °C / 21 °C (day / night) for 24 days with a 16 h light/8 h dark regime (light intensity was 300 μmol m-2 sec-1, the relative humidity was kept at 71 %). DNA was extracted with DNeasy Plant Pro Kit (Qiagen), bisulfite-treated with the EZ DNA Methylation-Gold Kit (Zymo Research), and prepared for sequencing with the xGen Methyl-Seq Lib Prep Kit (IDT DNA), according to standard protocols from the manufacturers. Unmethylated Lambda DNA (Promega) was added as a spike-in to verify bisulfite conversion efficiency. Libraries were sequenced (paired-end, 2x151 cycles, Illumina NovaSeq X Plus, IPK Gatersleben) following protocols provided by the manufacturer (Illumina).
sample_id
IPK_LIMS:3423432
species
cereale