Behnsdorf 4_048_R3S3 mtCOI metabarcoding from multiplexed PCR, NEB kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
NEB
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-14-R-TAIL-6
SampleName
048
Submitter Id
F-HEAD-14-R-TAIL-6.NEB.COI_lg
TagFWD
GCTGATC
TagREV
TACGCGA
adapters
FWD: GCTGATC REV: TACGCGA
broad-scale environmental context
ENVO:01000245
collection date
2020-10-09
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
52.30946301
geographic location (longitude)
11.18682100
lat
52.30946300805154
local environmental context
nest tube of cavity-nesting hymenoptera
lon
11.186821000501984
multiplex identifiers
FWD: GCTGATC REV: TACGCGA
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
project name
PilotNA
removal_date
2020-10-09
sample name
F-HEAD-14-R-TAIL-6.NEB.COI_lg
scientific_name
insect work metagenome
site
Behnsdorf
site_ID
BE
source material identifiers
4_048_R3S3
target gene
mtCOI
target subfragment
long barcoding region
trap_ID
4_048_R3S3