Rietzmeck 6_063_R3S1 ITS2 metabarcoding from multiplexed PCR, NEB kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
NEB
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-23-R-TAIL-6
SampleName
063
Submitter Id
F-HEAD-23-R-TAIL-6.NEB.ITS2_lg
TagFWD
TCAACCT
TagREV
TACGCGA
adapters
FWD: TCAACCT REV: TACGCGA
broad-scale environmental context
ENVO:01000245
collection date
2020-10-06
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
51.88923699
geographic location (longitude)
12.12432774
lat
51.889236994404975
local environmental context
nest tube of cavity-nesting hymenoptera
lon
12.124327743288525
multiplex identifiers
FWD: TCAACCT REV: TACGCGA
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
project name
PilotNA
removal_date
2020-10-06
sample name
F-HEAD-23-R-TAIL-6.NEB.ITS2_lg
scientific_name
insect work metagenome
site
Rietzmeck
site_ID
RI
source material identifiers
6_063_R3S1
target gene
ITS2
target subfragment
barcoding region
trap_ID
6_063_R3S1