CONTROL ITS2 metabarcoding from multiplexed PCR, NEB kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
NEB
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-17-R-TAIL-2
SampleName
CONTROL
Submitter Id
F-HEAD-17-R-TAIL-2.NEB.ITS2_lg
TagFWD
TCAGTTG
TagREV
AGCGTCT
adapters
FWD: TCAGTTG REV: AGCGTCT
broad-scale environmental context
missing: control sample
collection date
2022-02-01
environmental medium
missing: control sample
geographic location (country and/or sea)
Germany
geographic location (latitude)
missing: control sample
geographic location (longitude)
missing: control sample
local environmental context
missing: control sample
multiplex identifiers
FWD: TCAGTTG REV: AGCGTCT
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
project name
PilotNA
sample name
F-HEAD-17-R-TAIL-2.NEB.ITS2_lg
scientific_name
insect work metagenome
source material identifiers
CONTROL
target gene
ITS2
target subfragment
barcoding region