Dalchau 2_019_R3S3 mtCOI metabarcoding from multiplexed PCR, NEB kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
NEB
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-14-R-TAIL-5
SampleName
019
Submitter Id
F-HEAD-14-R-TAIL-5.NEB.COI_lg
TagFWD
GCTGATC
TagREV
GGGTAAT
adapters
FWD: GCTGATC REV: GGGTAAT
broad-scale environmental context
ENVO:01000245
collection date
2020-10-05
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
52.11862707
geographic location (longitude)
12.00216801
lat
52.11862706519449
local environmental context
nest tube of cavity-nesting hymenoptera
lon
12.002168005209025
multiplex identifiers
FWD: GCTGATC REV: GGGTAAT
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
project name
PilotNA
removal_date
2020-10-05
sample name
F-HEAD-14-R-TAIL-5.NEB.COI_lg
scientific_name
insect work metagenome
site
Dalchau
site_ID
DA
source material identifiers
2_019_R3S3
target gene
mtCOI
target subfragment
long barcoding region
trap_ID
2_019_R3S3