Lean Rat 37 HIF LongTerm
rat gut metagenome
collection_date
2022-11
env_broad_scale
gut(UBERON:0001007)
env_local_scale
colon(UBERON:0001155)
env_medium
feces(UBERON:0001988)
geo_loc_name
USA:Arkansas\,Little Rock (GAZ\,00165676)
host
Rattus norvegicus (FBsp:00000020)
lat_lon
not applicable
host_body_habitat
Gastrointestinal
host_body_product
feces
host_diet
Rats were fed a semi-purified diet similar to the AIN-93G diet, with casein as the protein source for a total of 8 weeks. Six-week-old male lean and obese (fa/fa) Zucker rats were purchased from Charles River Laboratories (Wilmington, MA). Rats were acclimated on AIN-93G diet for a week before being randomly assigned to the soy protein concentrate with high isoflavones (SPC-HIF diet) (Arcon SM; ADM; Decatur, IL) for 9 or 18 weeks (6 - 7 rats/group/time point). SPC-HIF diets had 2.153 mg total isoflavone/g protein All diets were isocaloric and isonitrogenous.
host_genotype
fa/fa
host_phenotype
Lean
host_sex
male
host_subject_id
37
host_taxid
10116
samp_mat_process
Total microbial DNA was collected directly from the fecal samples using a PowerSoil® DNA isolation kit (QIAGEN, Germantown, MD). Isolated DNA was used for amplifying the V4 region of 16S rRNA gene as per Kozich et al. (2013) Data was collected using the Illumina NextSeq 2000.
samp_store_temp
-80 C
illumina_sample_id
27
AST
236
ALT
30
PathScore
0
FinalBodyWeight_g
437
LPSBindingProtein
3.469861743643887
TNFa
2.129662671254157
MCP1
1.7995350108943482
iNOS
7.620548308247328
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.host-associated