Steuden 8_090_R2S3 ITS2 metabarcoding from multiplexed PCR, Qiagen kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
QIA
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-8-R-TAIL-4
SampleName
090
Submitter Id
F-HEAD-8-R-TAIL-4.QIA.ITS2_lg
TagFWD
TTTGCTA
TagREV
CTGCGAG
adapters
FWD: TTTGCTA REV: CTGCGAG
broad-scale environmental context
ENVO:01000245
collection date
2020-10-08
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
51.40237504
geographic location (longitude)
11.78100500
lat
51.402375041368984
local environmental context
nest tube of cavity-nesting hymenoptera
lon
11.78100500295243
multiplex identifiers
FWD: TTTGCTA REV: CTGCGAG
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
project name
PilotNA
removal_date
2020-10-08
sample name
F-HEAD-8-R-TAIL-4.QIA.ITS2_lg
scientific_name
insect work metagenome
site
Steuden
site_ID
ST
source material identifiers
8_090_R2S3
target gene
ITS2
target subfragment
barcoding region
trap_ID
8_090_R2S3