Behnsdorf 4_045_R4S2 ITS2 metabarcoding from multiplexed PCR, Qiagen kit
insect work metagenome
ENA first public
2025-08-01
INSDC center name
Thuenen-Institute of Biodiversity
INSDC status
public
Marker
QIA
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_2
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
SampleID_ASV
F-HEAD-4-R-TAIL-2
SampleName
045
Submitter Id
F-HEAD-4-R-TAIL-2.QIA.ITS2_lg
TagFWD
CACTTAT
TagREV
AGCGTCT
adapters
FWD: CACTTAT REV: AGCGTCT
broad-scale environmental context
ENVO:01000245
collection date
2020-10-09
environmental medium
ENVO:02000004
geographic location (country and/or sea)
Germany
geographic location (latitude)
52.32013601
geographic location (longitude)
11.16393100
lat
52.320136007439096
local environmental context
nest tube of cavity-nesting hymenoptera
lon
11.163931000461703
multiplex identifiers
FWD: CACTTAT REV: AGCGTCT
nucleic acid amplification
PCR
nucleic acid extraction
Machery-Nagel NucleoSpin 96 Soil Kit
pcr primers
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
project name
PilotNA
removal_date
2020-10-09
sample name
F-HEAD-4-R-TAIL-2.QIA.ITS2_lg
scientific_name
insect work metagenome
site
Behnsdorf
site_ID
BE
source material identifiers
4_045_R4S2
target gene
ITS2
target subfragment
barcoding region
trap_ID
4_045_R4S2