CO-SC-HE-03-LN
soil metagenome
ENA first public
2025-03-31
INSDC center name
Scotland's Rural College - Aberdeen Campus
INSDC status
public
Submitter Id
ms00548
adapters
Forward overhang: 5'-AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT; Reverse overhang: 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATGACTATCTCGTATGCCGTCTTCTGCTTG
broad-scale environmental context
environmental system
chimera check software
N/A
climate environment
controlled glasshouse
collection date
2021-12-30
environmental medium
bulk soil
experimental factor
bulk soil no plant control
geographic location (country and/or sea)
United Kingdom
geographic location (latitude)
51.8094
geographic location (longitude)
0.3561
host age
3
host common name
N/A
host life stage
N/A
host subspecific genetic lineage
N/A
host taxid
410658
local environmental context
soil environment
multiplex identifiers
N/A
negative control type
N/A
nucleic acid amplification
https://www.neb.com/en-gb/protocols/2014/05/22/protocol-for-use-with-nebnext-ultra-dna-library-prep-kit-for-illumina-e7370
nucleic acid extraction
Qiagen(TM) Power soil gDNA extraction kit
pcr conditions
98�C for 30 seconds; 10 cycles of 98�C for 10 seconds, 65�C for 75 seconds; 65�C for 5 minutes; Hold at 4�C
pcr primers
N/A
plant body site
N/A
plant product
N/A
positive control type
synthetic community of three known bacterial species
project name
UK Crop Microbiome Cryobank
reference for biomaterial
Rothamsted Research Station
relevant electronic resources
agmicrobiomebase.org
sample name
ms00548
sample volume or weight for DNA extraction
0.5
scientific_name
soil metagenome
sequence quality check
software
sequencing method
Novaseq 6000
target gene
N/A