G500022_HAP1_CREBBP1_S22
Homo sapiens
Protocols: HAP1 parental and KO cell lines (RRID:CVCL_Y019) for ARID1A, ARID1B, ARID2, PBRM1, SMARCA2, SMARCA4, KMT2C, KMT2D, CREBBP or SETD2 were purchased from Horizon Discovery. HAP-1 KO cell lines for SMARCB1, BAP-1 or EED were obtained from Drs. Raphaël Margueron (Institut Curie, UMR934, Paris) and Eric Pasmant's teams (Institut Cochin, Paris).  HAP-1 cells were cultured in Iscove's Modified Dulbecco's Medium (IMDM, Gibco) supplemented with 10% FBS (Sigma), 1% Penicillin-Streptomycin (Gibco), 1% Sodium Pyruvate (Gibco), 1% Sodium Bicarbonate (Gibco) and 1% Non-Essential Amino Acids (Gibco).  70-80% confluent cells were harvested, and total RNA was extracted using Rneasy Mini Kit (Qiagen, 74104) with DNAse treatment, according to the manufacturer's instructions. Every RNA sample was quantified with a Qubit Fluorometer and evaluated for quality controls using Agilent 2100 Bioanalyzer Instrument (RRID:SCR_018043). After RNA Integrity Number (RIN) quality control, cDNA libraries were generated using the NEBNext Ultra II RNA Library Prep Kit (NEB #E7775) on Bravo Automated Liquid Handling Platform (RRID:SCR_026137).
ENA first public
2025-10-30
INSDC center name
Institut Gustave-Roussy
INSDC status
public
Submitter Id
E-MTAB-15673:G500022_HAP1_CREBBP1_S22
broker name
ArrayExpress
cell line
HAP-1
cell type
neoplastic cell
collection date
not collected
common name
human
developmental stage
adult
disease
chronic myeloid leukemia
genotype
CREBBP_KO
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blood
replicate
1
sample name
E-MTAB-15673:G500022_HAP1_CREBBP1_S22
scientific_name
Homo sapiens