Sample 6
Bordetella pertussis
Protocols: Bacterial suspensions (8 ml) were collected at mid-exponential phase and centrifuged at 4,000 rpm for 15 minutes at 4°C after adding 2 mL of a phenol:ethanol solution (5:95, v/v). The bacteria were grown with or without agitation in SS medium supplemented with 20 mM MgSO4. Total RNA was extracted using Tri-Reagent, followed by two consecutive DNase I treatments, each followed by purification using AMPure XP beads to remove genomic DNA and contaminating solvents. Strand-specific RNA-seq libraries were prepared with the QIAseq Stranded RNA Library Kit according to the manufacturer's instructions.
ENA first public
2026-03-31
INSDC center name
IPL_U1011
INSDC status
public
Submitter Id
E-MTAB-16727:Sample 6
broker name
ArrayExpress
collection date
not collected
geographic location (country and/or sea)
not collected
growth condition
Low aeration
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-16727:Sample 6
scientific_name
Bordetella pertussis
strain
BPSM