Sample 1
Homo sapiens
Protocols: The CRISPR-Pool SAM human library, containing 70290 sgRNAs targeting 23430 genes were constructed in MS2-P65-HSF1. After the cells were treated with apatinib for another week and their genomic DNA was extracted. Total DNA were extracted using NEBNext Ultra II DNA Kit The sgRNA fragments were amplified by PCR. After passing quality control, 20-50 ng of DNA fragments are used to construct a high-throughput sequencing library for the Illumina platform through steps such as End Repair, 5 Phosphorylation and dA-Tailing, Adapter Ligation, Clean Up, and PCR Enrichment and Clean Up.
ENA first public
2025-10-30
INSDC center name
The Second Hospital & Clinical Medical School, Lanzhou University
INSDC status
public
Submitter Id
E-MTAB-15686:Sample 1
broker name
ArrayExpress
cell line
HUVEC cell line
cell type
endothelial cells
collection date
not collected
common name
human
developmental stage
adult
disease
cancer
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
vasculature
sample name
E-MTAB-15686:Sample 1
scientific_name
Homo sapiens