Thermus thermophilus HB27c cells expressing lalcas12r-repeat-18 nt spacer against phiKo phage
Thermus thermophilus
Type V CRISPR-Cas locus derived from Lalka phages was cloned into pMK18 vector along with the single repeat unit followed by 18 nt long spacer against phiKo. The plasmid was introduced into T. thermophilus HB27c cells. Small RNAs were isolated from the cell cultures and sequenced. The experiment was performed in two replicates.
strain
HB27c
isolation_source
laboratory
collection_date
2025-10-07
geo_loc_name
Russia:Moscow
sample_type
cell culture
BioSampleModel
Microbe, viral or environmental