Potter Cove sediment slurry SIP incubation - 13C Acetate.Lepidocrocite at 2 degrees C - nucleic acid extract
marine sediment metagenome
Potter Cove sediment slurry SIP incubation with 13C labeled acetate and lepidocrocite at 2 degree Celsius, nucleic acid extract, sediment from station 13, core STA13.04, Potter Cove, Antarctica; parameters geographic location, depth and collection date  refer to sediment used to prepare the slurry; experiment set up in biological triplicates and pooled for extraction
ENA first public
2026-05-07
INSDC center name
UniBremen
INSDC status
public
Submitter Id
2degrees.13C.Acetate.Lepidocrocite.notfrac.DNA
amount or size of sample collected
0.045
broad-scale environmental context
shallow marine sediment [ENVO:03000034] | polar biom [ENVO:01000339] | glacial retreat [ENVO:01001656] | coast [ENVO:01000687]
chem_administration
acetate, lepidocrocite
collection date
2019-01-23
depth
0 - 0.29
elevation
-15
environmental medium
microbial community [PCO:1000004] | mesocosm [ENVO:01000620]
experimental factor
different substrate additions | RNA stable isotope probing | exposure temperature [EFO:0000718] | response to heat [GO:0009408] | labelling [EFO:0000562]
geographic location (country and/or sea)
Antarctica
geographic location (latitude)
-62.2253889
geographic location (longitude)
-58.6411667
geographic location (region and locality)
King George Island/Isla 25 de Mayo, Potter Cove
local environmental context
anaerobic sediment [ENVO:00002045]
oxygenation status of sample
anaerobic
project name
Bacterial 16S rRNA community in acetate degrading stable isotope probing slurry incubation experiment across temperature range with sediment of Potter Cove
relationship to oxygen
anaerobe
sample collection device
syringe, needle
sample collection method
slurry sampling in anoxic manner
sample material processing
slurry triplicates were pooled and sample was frozen at -80 degrees C until nucleic acid extraction
sample name
2degrees.13C.Acetate.Lepidocrocite.notfrac.DNA
sample volume or weight for DNA extraction
45
scientific_name
marine sediment metagenome
source material identifiers
slurry prepared in ratio 1:6 of sediment Potter Cove core STA13.04 and sulfate-free artificial sea water