N5U-mineral-bacteria
soil metagenome
Norway ungrazed mineral
ENA first public
2026-01-29
External Id
SAMEA115160640
INSDC center name
University of Oulu
INSDC last update
2024-01-29T11:58:47Z
INSDC status
public
Submitter Id
ena-SAMPLE-TAB-29-01-2024-11:58:39:695-58903
broad-scale environmental context
sub-arctic boreal forest
collection date
2015-08-15
composite design/sieving (if any)
five soil cores pooled together
current land use
winter reindeer grazing
current vegetation
mountain birch with an understory layer dominated by dwarf shrubs (Empetrum nigrum ssp. hermaphroditum, Vaccinium myrtillus, V. vitis-idaea)
depth
0.02-0.05
elevation
115
environmental medium
soil
geographic location (country and/or sea)
Norway
geographic location (latitude)
70.036
geographic location (longitude)
27.98238
geographic location (region and locality)
Polmak
history/extreme events
severe moth outbreaks 2002-2008
local environmental context
mountain birch forest
mean annual and seasonal precipitation
annual 450 mm
mean annual and seasonal temperature
seasonal 12C and -12C in the warmest (July) and coldest (January) months respectively
pH
4,6
pcr conditions
initial denaturation:98degC_1min; (denaturation:98degC_10sec; annealing:64degC_30sec; elongation:72degC_30sec)cycles=26; final elongation:72degC_7min
pcr primers
forward primer:CAGCMGCCGCGGTAATWC;reverse primer:CCGTCAATTCCTTTRAGTTT
project name
Polmak soil 2015
sample collection device
soil corer 3 cm diameter
sample material processing
humus and mineral soil samples separated in the field, stored at outdoor temperature during sampling, stored at -20 degrees C after sampling
sample name
ena-SAMPLE-TAB-29-01-2024-11:58:39:695-58903
sample volume or weight for DNA extraction
0.5548
scientific_name
soil metagenome
sequencing method
Ion Torrent PGM
soil horizon
B horizon
storage conditions (fresh/frozen/other)
frozen -20 degrees C
target gene
16S
target subfragment
V4-V5