Sample 4
Mus musculus
Protocols: Single cell suspensions of the lung were obtained and cells were stained for 30 minutes at 4 degrees celcius before sorting. Following sorting strategy was used: live cells, CD45 IV negative cells. For each sample, 7500 immune cells were sorted (CD45+) and 7500 structural cells (CD45-, CD31-, Ter119-, EPCAM+) Single live cells were sorted and loaded onto a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads in emulsion (GEMs) The scRNA-Seq libraries were prepared using the GemCode Single Cell 3' Gel Bead and Library kit, version NextGEM 3.1 (10x Genomics) according to the manufacturer's instructions
ENA first public
2025-10-30
INSDC center name
Laboratory of Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium
INSDC status
public
Submitter Id
E-MTAB-15850:Sample 4
age
15
broker name
ArrayExpress
cell type
dendritic cell
collection date
not collected
common name
house mouse
developmental stage
Alveolarisation phase lung
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lung
sample name
E-MTAB-15850:Sample 4
scientific_name
Mus musculus
sex
male
stimulus
PVM - HDM
strain
C57Bl6/J