tail6
Mus musculus
Protocols: Tumor samples and matched normal tissue from the same donor were collected for whole exome sequencing analysis. Genomic DNA was isolated from tumor material and corresponding normal tissue for paired somatic variant calling. Genomic DNA was extracted from tumor and matched normal tissue samples. A total of 200 ng gDNA per sample was used for downstream library preparation. Whole exome sequencing libraries were generated using the SureSelectXT Automation Reagent Kit according to the manufacturer's instructions. Briefly, 200 ng of genomic DNA was fragmented to approximately 150 bp using a Covaris LE220 ultrasonicator. Library preparation was performed on a Bravo automated liquid handler and included end repair, A-tailing, adaptor ligation, and amplification. Library concentration was assessed using the Agilent TapeStation. Subsequently, 750 ng of amplified adaptor-ligated DNA library was hybridized with the SureSelectXT All Exon v7 bait set. DNA-library/bait hybrids were captured using streptavidin-coated magnetic beads. Index tags were added during PCR amplification of the captured libraries. Final libraries were validated using Qubit and Agilent TapeStation.
ENA first public
2026-06-12
INSDC center name
Researcher, German Cancer Resarch Center
INSDC status
public
Submitter Id
E-MTAB-17103:tail6
age
>6
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
WT
geographic location (country and/or sea)
not collected
individual
6
isolate
not applicable
organism part
tail
sample name
E-MTAB-17103:tail6
scientific_name
Mus musculus
sex
unkown
strain
C57BL/6