Dog_gut_microbiome_DMD_D_PF1_230225_Narancs3
Canis lupus familiaris
Dog stool sample of Narancs
ENA first public
2026-03-31
INSDC center name
UNIVERSITY OF SZEGED
INSDC status
public
Submitter Id
Canfam_stool_DMD_D_PF1_230225_Narancs3
adapters
5'-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3'
biological status
breeder's line
broad-scale environmental context
Danube Basin
chimera check software
Kraken2
collection date
2023-02-25
common name
dog
environmental medium
no
geographic location (country and/or sea)
Hungary
geographic location (latitude)
46.181793
geographic location (longitude)
18.954306
geographic location (region and locality)
Baja
gravidity
non gravid
host age
4.43
host body product
stool
host common name
Hungarian herding terrier: Pumi
host diet
Josera Family Plus and breastmilk
host family relationship
Puppy of Boszi - Litter D
host life stage
puppy
host scientific name
Canis lupus familiaris
host sex
female
host subject id
DMD_D_Narancs
host subspecific genetic lineage
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host taxid
9615
library construction method
16S Barcoding Kit (SQK-16S024)
library screening strategy
AMPLICON
library size
1500 bp
local environmental context
garden/house
multiplex identifiers
AACGAGTCTCTTGGGACCCATAGA
nucleic acid amplification
LongAmp Taq 2X Master Mix
nucleic acid extraction
Macherey Nagel NucleoSpin DNA Stool kit
pcr conditions
Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
pcr primers
27F 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R 5'-CGGTTACCTTGTTACGACTT-3'
project name
Multiplatform metagenomic sequencing for longitudinal analysis of canine gut microbiome
sample material processing
none
sample name
Canfam_stool_DMD_D_PF1_230225_Narancs3
sample storage location
Eppendorf_CryoCube_F740h
sample storage temperature
-80
sample volume or weight for DNA extraction
0,2
scientific_name
Canis lupus familiaris
target gene
16S rRNA
target subfragment
V1-V9
temperature
7