ARHI1R1
Homo sapiens
Protocols: Cells were lyzed directly to cell culture vessels with TRIzol reagent (Thermo Fisher Scientific) For androgen induction studies, RWPE-1 cells were grown in phenol red-free RPMI-1640 medium with 5% charcoal/dextran-treated (CCS) FBS and 1% glutamine for 3 days. LNCaP were grown in same medium but with 10% charcoal/dextran-treated(CCS) FBS. For androgen induction studies, hormone-deprived cells were treated with 0, 1, 10 or 100 nM of dihydrotestosterone (DHT) for 4 hours before cell lysis. TRIzol protocol (Thermo Fisher Scientific) + RNEasy kit (Qiagen) for on-column DNase I treatment with RNase-Free DNase Set (Qiagen) Standard Illumina polyA enrichment libraries, done by Novogene in the seuquencing center in Hong Kong, or in Cambridge Sequencing Center
ENA first public
2026-03-31
INSDC center name
Faculty of Medicine and Health Technology, Tampere University and Tays Cancer Centre, Tampere University Hospital, Tampere, Finland
INSDC status
public
Submitter Id
E-MTAB-15758:ARHI1R1
broker name
ArrayExpress
cell line
LNCaP-ARhi
cell type
prostate gland cancer cell
collection date
not collected
common name
human
developmental stage
adult
disease
prostate cancer
genetic modification
pcDNA3.1-AR
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
prostate gland
replicate
2
sample name
E-MTAB-15758:ARHI1R1
scientific_name
Homo sapiens
stimulus
1_nM_DHT