RNP FP 2
Homo sapiens
Protocols: Wild type and KO Jurkat cells (American Type Culture Collection, Inc., ATCC) were cultured in 1× Roswell Park Memorial Institute Medium (RPMI) 1640. Total RNA was extracted and purified from HSPCs after cell lysis (~400,000 cells per condition in triplicates) using a Qiagen RNeasy Mini Kit.  RNA libraries for RNA-seq were prepared using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads following the manufacturer's protocol which included reverse transcription of mRNA, ligation of adaptors, and PCR enrichment of libraries. Libary pool was 10nM.
ENA first public
2025-10-30
INSDC center name
Department of Bioengineering UCLA
INSDC status
public
Submitter Id
E-MTAB-15741:RNP FP 2
broker name
ArrayExpress
cell type
hematopoietic stem and progenitor cell
collection date
not collected
common name
human
developmental stage
adult
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
peripheral blood
sample name
E-MTAB-15741:RNP FP 2
scientific_name
Homo sapiens
stimulus
filtroporation, with cargo, rep 2