1-F-E2R
Homo sapiens
Protocols: Following hormone treatment, the culture media in 60-mm dishes were removed thoroughly. 1ml of TRIzol was added to each plate for cell lysis. The cultured cells were lysed in cold TRIzol reagent, and total RNA was isolated according to the manufacturer's protocol (Life Technologies, MD, USA). RNA was precipitated using isopropanol and pelleted at 12000xg, 4°C for 15 minutes. The pelleted RNA was washed twice with 75% ethanol prepared in DEPC-treated water, briefly air-dried and resuspended in DEPC-treated water.  . DNA free RNA samples were submitted to Genome Institute of Singapore (Agency for Science, Technology and Research) for library preparation and paired-end sequencing using Illumina HiSeq4000 platform.
ENA first public
2026-03-31
INSDC center name
NTU
INSDC status
public
Submitter Id
E-MTAB-16059:1-F-E2R
broker name
ArrayExpress
cell line
MCF-7
cell type
FFF-mutant, Estrogen+R5020 treated, Replicate 1
collection date
not collected
common name
human
disease
breast adenocarcinoma
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Breast
sample name
E-MTAB-16059:1-F-E2R
scientific_name
Homo sapiens