Het32_CTCF_ChIP
Mus musculus
Protocols: BMEL cells were crosslinked by a double procedure of 2 mM Di-Succinimidyl-Glutarate (DSG, Merck) and 1% formaldehyde. Chromatin was sonicated for 15 cycles of 30 seconds on and 30 seconds off. Following extraction, chromatin immunoprecipitation (ChIP) was incubated overnight with 4 µg of antibody. followed by the addition of protein G beads (Dynabeads) incubated for an additional 1h. Beads were then washed 5 times for 5 min each on a rotating wheel. Samples were incubated overnight at 65°C to reverse crosslink, followed by proteinase K and RNase A treatment. DNA was isolated using a NucleoSpin PCR purification kit (Macherey Nagel). ChIP-seq libraries were prepared with DNA Library Prep Kit for Illumina by the Genom'IC core facility (Paris, France).
ENA first public
2026-03-31
INSDC center name
Sorbonne Université Institut de Biologie Paris-Seine CNRS UMR7238 - Computational, Quantitative and Synthetic Biology
INSDC status
public
Submitter Id
E-MTAB-15196:Het32_CTCF_ChIP
broker name
ArrayExpress
cell line
Het32
cell type
BMEL
collection date
not collected
common name
house mouse
developmental stage
mouse embryo stage
genotype
Heterozygote Cbx1+/+ Cbx3+/+ Cbx5+/-
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
liver
sample name
E-MTAB-15196:Het32_CTCF_ChIP
scientific_name
Mus musculus