dog_stool_microbial_sample_Loki7
Canis lupus familiaris
Dog stool sample of Loki
ENA first public
2026-03-31
INSDC center name
UNIVERSITY OF SZEGED
INSDC status
public
Submitter Id
Canfam_stool_DMD_0_AM1_211227_WGS_Loki7
adapters
CTGTCTCTTATACACATCT
broad-scale environmental context
Danube Basin
collection date
2021-12-27
common name
dog
environmental medium
no
geographic location (country and/or sea)
Hungary
geographic location (latitude)
46.181793
geographic location (longitude)
18.954306
geographic location (region and locality)
Baja
host age
383.71
host body product
stool
host common name
Hungarian herding terrier: Pumi
host diet
BARF
host family relationship
Control dog, same living environment
host life stage
adult
host scientific name
Canis lupus familiaris
host sex
male
host subject id
not applicable
host subspecific genetic lineage
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host taxid
9615
library construction method
Illumina DNA Prep Kit (Document #1000000025416v10)
library screening strategy
WGS
library size
300 bp
local environmental context
garden/house
multiplex identifiers
CGTACTAG / GTAAGGAG
nucleic acid amplification
Enhanced PCR Mix (EPM)
nucleic acid extraction
Macherey Nagel NucleoSpin DNA Stool kit
pcr conditions
3 min 68 �C (1 cycle); 3 min 98 �C (1 cycle); 45 secs 98 �C (5 cycles); 30 secs 62 �C (5 cycles); 2 mins 68 �C (5 cycles); 1 min 68 �C (1 cycle); Hold at 10 �C
pcr primers
Index 1 Read: 5? CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGG; Index 2 Read: 5? AATGATACGGCGACCACCGAGATCTACAC[i5]TCGTCGGCAGCGTC
project name
Multiplatform metagenomic sequencing for longitudinal analysis of canine gut microbiome
sample name
Canfam_stool_DMD_0_AM1_211227_WGS_Loki7
sample storage location
Eppendorf_CryoCube_F740h
sample storage temperature
-80
sample volume or weight for DNA extraction
0,2
scientific_name
Canis lupus familiaris
target gene
whole genom
target subfragment
whole genom
temperature
3