Sample 2
Homo sapiens
Protocols: Samples of orbital fat were taken during surgery for planned or emergency orbital decompressions To isolate single cells from the orbital fat, it was cut into small (2-3mm diameter) pieces and digested for 15 minutes in Hank's buffered saline solution (HBSS) containing 2mg/ml collagenase P, 1mg/ml dispase and 1ul/ml DNaseI (100u/ml) at 37C with shaking. Samples were centrifuged and the fraction containing PBMCs and fibroblasts was frozen in CryostorCS10 (StemCell Technologies). Samples were then thawed, washed (BioLegend) and run on a 10xgenomics Single Cell 5' Reagent kit v2.  Standard 10x V2 library construction. Cell hashing was insufficient but included for completeness
ENA first public
2026-06-26
INSDC center name
Cardiff University, University of Birmingham
INSDC status
public
Submitter Id
E-MTAB-16698_2:Sample 2
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
Graves ophthalmopathy
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
orbital fat
sample name
E-MTAB-16698_2:Sample 2
scientific_name
Homo sapiens