RNAseq_plusNB_b
Cereibacter sphaeroides
Protocols: All cells were harvested by high speed centrifugation (approx 5000 xG). Pellets were flash froze in liquid nitrogen before storage at -80 oC. For each biological replicate, a single colony was inoculated into 10 ml M22+ media and grown at 30 degrees Celsius with vigorous shaking for 2-3 days. This 10 ml culture was used to start a larger culture, typically 100 - 200 ml. The larger culture had a starting OD600 of 0.05, and was grown throughout the day at 30 degrees Celsius with shaking until exponential phase, approx 0.5 OD600. At which point novobiocin was added for 20 minutes if required.  When stated, novobiocin was added to a exponentially growing cells (OD600 0.5) to a final concentation of 2 μg/ml Total RNA was isolated as described previously (1) with the exception that RNA concentration and quality was determined on an Agilent 2200 Tapestation.   1 - Forrest, D., James, K., Yuzenkova, Y. & Zenkin, N. Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase. Nat. Commun. 8, 15774 (2017) Library construction for strand-specific RNA-seq was performed Gene Wiz (Azenta)
ENA first public
2025-10-30
INSDC center name
Institute for Microbiology and Infection, University of Birmingham
INSDC status
public
Submitter Id
E-MTAB-15087:RNAseq_plusNB_b
broker name
ArrayExpress
collection date
not collected
geographic location (country and/or sea)
not collected
growth condition
M22+ media. 30 degrees Celsius
isolate
not applicable
replicate
biological replicate b
response to treatment
2 μg/ml novobiocin
sample name
E-MTAB-15087:RNAseq_plusNB_b
scientific_name
Cereibacter sphaeroides
strain
Rhodobacter sphaeroides 2.4.1