Sample 2
Drosophila melanogaster
Protocols: To eliminate/reduce yeast (and yeast RNA) in the intestine, flies were transferred to bottles containing filter paper saturated with 5% glucose in water for four hours. The flies were anesthetized by CO2 and the entire intestine excluding the crop was excised in PBS. Isolated guts were then placed into Eppendorf tubes. Total RNA was isolated from 30 female guts using Qiagen's RNeasy Plus kit. RNA sample quality assessment was performed with the Fragment Analyzer Standard Sensitivity RNA assay (Agilent) and concentration measured with the Qubit 3.0 HS RNA assay (Thermo).   NGS libraries were prepared with the Stranded mRNA library prep kit (Illumina) using 400 ng of total RNA input.
ENA first public
2025-10-30
INSDC center name
Ottawa Hospital Research Institute
INSDC status
public
Submitter Id
E-MTAB-15806:Sample 2
broker name
ArrayExpress
collection date
not collected
common name
fruit fly
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
gut
sample name
E-MTAB-15806:Sample 2
scientific_name
Drosophila melanogaster
sex
female