LCP92_Coculture
Homo sapiens
Protocols: Patient lung cancer samples, including tumor tissues and adjacent normal tissues, were obtained from the Department of Thoracic Surgery at Peking University Shenzhen Hospital. Written informed consent was obtained from all participants prior to sample collection. All procedures were approved by the Ethics Committee of Peking University Shenzhen Hospital (NO.2020-057). Tissues were washed with DPBS containing 1% penicillin/streptomycin. Non-target tissues were manually removed before digestion. Nucleic acid extraction was performed as an integrated part of the single-cell library preparation protocol using the DNBelab C4 Single-Cell Library Prep Set (MGI). Within the microfluidic system, individual cells were encapsulated in droplets with barcoded beads. Cell lysis occurred within the droplets, and poly-adenylated mRNA molecules were captured onto the oligo-dT primers on the surface of the beads. Single-cell RNA-seq libraries were prepared using the DNBelab C4 Single-Cell Library Prep Set (MGI) according to the manufacturer's instructions. Following mRNA capture on barcoded beads, emulsion was broken and beads were recovered. Full-length cDNA was synthesized via reverse transcription. The resulting cDNA was then amplified to generate the final single-cell sequencing libraries.
ENA first public
2025-10-30
INSDC center name
PKUSZH
INSDC status
public
Submitter Id
E-MTAB-15784:LCP92_Coculture
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
IIB
disease
LUSC
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-15784:LCP92_Coculture
sample type
Co-Culture
scientific_name
Homo sapiens
sex
male