mmus-DT_2-skin-c8
Mus musculus
Protocols: DNA was isolated from 1 mm3 mouse back skin biopsies and livers using a modified and combined version of the AllPrep DNA/RNA Micro Kit (Qiagen) and DNeasy Blood and Tissue Kit (Qiagen).  350 μl RLT buffer (from the AllPrep DNA/RNA Micro Kit) containing 1% β-Mercaptoethanol (Sigma-Aldrich) was added to each sample (containing one biopsy) and the tissue was homogenised in a Qiagen TissueLyzer II using 2 cycles of 3 minutes at 30 Hz. After lysis 688 μl of nuclease-free water and 12 μl proteinase K (from DNeasy Blood and Tissue Kit) was added to the lysate and incubated at 56°C for 10 minutes. Half of the sample (519 ul) was used for DNA extraction. 519 μl Buffer AL (Qiagen Blood & Tissue) was added, mix by vortexing, and incubated at 56°C for 30 minutes at 500 rpm. An equal volume (519 μl) of absolute ethanol was added to samples and mixed thoroughly. The precipitated mixture was transferred to a DNeasy Mini spin 96-well spin plate (Qiagen), washed twice with 700 μl buffer AW1 and twice with buffer AW2. After drying the membrane for 3 minutes at 20,000 x g DNA was eluted using two consecutive elutions of 30 µl pre-warmed AE buffer (70°C). The quality of the DNA was assessed using a Genomic DNA ScreenTape (Agilent Technologies).
ENA first public
2026-06-12
INSDC center name
German Cancer Research Center (DKFZ)
INSDC status
public
Submitter Id
E-MTAB-15745:mmus-DT_2-skin-c8
age
19
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
T2
isolate
not applicable
organism part
back skin
sample name
E-MTAB-15745:mmus-DT_2-skin-c8
scientific_name
Mus musculus
sex
female
strain
C57BL/6J