CLKdKO4
Homo sapiens
Protocols: Wild-type and mutant hESCs were maintained in mTeSR™ Plus medium (STEMCELL Technologies, Vancouver, Canada) on culture plates coated with diluted Matrigel (Corning Inc., Corning, NY, USA) in DMEM/F-12 medium (Thermo Fisher Scientific Inc., Waltham, MA, USA). Colonies were passaged every 5–7 days using ReLeSR™ (STEMCELL Technologies) and resuspended in medium supplemented with 10 μM Y-27632 (STEMCELL Technologies).  Total RNA was extracted from hESCs using the miRNeasy Mini Kit (Qiagen), and RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies). Ribosomal RNA was depleted using the Ribo-Zero™ reagent (Illumina). cDNA libraries were prepared with the TruSeq™ Stranded Total RNA Prep Kit (Illumina) according to the manufacturer's protocol.
ENA first public
2026-03-31
INSDC center name
Department of Anatomy, College of Medicine, Korea University
INSDC status
public
Submitter Id
E-MTAB-16296:CLKdKO4
broker name
ArrayExpress
cell line
H1(WA01) hESC
cell type
embryonic stem cell
collection date
not collected
common name
human
genotype
CLOCK-truncated
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blastocyst
sample name
E-MTAB-16296:CLKdKO4
scientific_name
Homo sapiens