PRBC2
Homo sapiens
Protocols: After treatment, cells were crosslinked with formaldehyde at a final concentration of 1% for 10 minutes at room temperature. The cross-linking was quenched by the addition of glycine to a final concentration of 0.125 M. Cells were then harvested, lysed, and subjected to sonication using Diagenode Bioruptor Pico.  Chromatin immunoprecipitation was performed using anti-PR antibody H-190 (Santa Cruz Biotechnology Inc., Dallas, TX, USA, LOT number sc-7208). The lysate was incubated with 5 ug anti-PR antibody overnight at 4 °C. Protein-chromatin complex was captured using Pierce™ ChIP-grade Protein A/G Magnetic Beads (ThermoFisher Scientific, Cat. #26162). Chromatin was eluted, and crosslinks were reversed by treatment with RNase A and Proteinase K. Input samples and ChIP'ed DNA was purified using QIAquick PCR Purification Kit (Qiagen). DNA concentrations were quantified using Qubit Fluorometer (ThermoFisher Scientific). The samples were submitted to Genome Institute of Singapore (Agency for Science, Technology and Research) for library preparation and paired-end sequencing using Illumina HiSeq4000 platform.
ENA first public
2026-03-31
INSDC center name
NTU
INSDC status
public
Submitter Id
E-MTAB-15999:PRBC2
broker name
ArrayExpress
cell line
MCF-7
cell type
PR-overexpressed cell, ETOH-treated, Replicate 2
collection date
not collected
common name
human
disease
breast adenocarcinoma
genotype
PR overexpressed
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
breast
sample name
E-MTAB-15999:PRBC2
scientific_name
Homo sapiens