Sample 19
Mus musculus
Protocols: Cells were passaged every 2-3 days to maintain cultures between 10% and 90% confluency. For analysis, near-confluent cultures were released from culture vessels with trypsin, spun down, and snap-frozen in liquid nitrogen before further processing. E14tg2a mouse embryonic stem cells were grown in dishes pre-coated with 0.1 % gelatin (w/vol, Sigma) in GMEM supplemented with 10% fetal bovine serum, sodium pyruvate, 50 μM β-mercaptoethanol, glutamax, non-essential amino acids (all from Gibco/ThermoFisher) and 10 ng/ml murine leukemia inhibitory factor (LIF, Protein Expression Facility, MPI Dortmund). gDNA was isolated from E12tg2a mESCs using the Monarch Genomic DNA Purification Kit (NEB, T3010) according to the manufacturer's instructions. gDNA was sheared to an average fragment size of 200 bp using a Bioruptor Pico sonication device (Diagenode) Dyad Cap libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit. DNA purification and size selection was carried out using NEBNext Sample Purification Beads according to the protocol with bead volumes corresponding to a target fragment size of 200 bp. Fragment size distribution was verified using a TapeStation.
ENA first public
2026-04-14
INSDC center name
Max Planck Institute of Molecular Physiology
INSDC status
public
Submitter Id
E-MTAB-15857:Sample 19
broker name
ArrayExpress
cell line
ES-E14 cell
cell type
mouse embryonic stem cell
collection date
not collected
common name
house mouse
developmental stage
mouse embryo stage
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
mecp2 variant
MECP2 HM
sample name
E-MTAB-15857:Sample 19
scientific_name
Mus musculus