leukemic_line_12
Mus musculus
Protocols: Samples of 5 in vitro transformed Ink4a/Arf KO NK cell lines (2 Ink4a/Arf -/- and 3 Ink4a/ARF-/- Rag2-/-) and 12 leukemic Ink4a/Arf KO NK cell lines (6 Ink4a/Arf -/- and 6 Ink4a/Arf-/- Rag2-/- ) were harvested, pelleted, snap-frozen and stored at -80°C. Samples of NK cells isolated from the spleen of healthy/untransformed mice were cultured in the presence of IL2 for 6-8 days and then harvested, pelleted, snap-frozen and stored at -80°C. DNA was isolated from snap frozen cell pellets using the Monarch Genomic DNA Purification Kit (New England Biolabs). Library preparation via enzymatic fragmentation and target enrichment by hybridization was performed using the Twist Bioscience Kit with Twist Mouse Exome panel (Twist Bioscience).
ENA first public
2026-03-31
INSDC center name
University of Veterinary Medicine Vienna Department of Biological Sciences and Pathobiology
INSDC status
public
Submitter Id
E-MTAB-16288:leukemic_line_12
broker name
ArrayExpress
cell type
natural killer cell
collection date
not collected
common name
house mouse
culture id
line_5
developmental stage
juvenile days 45-89
disease
leukemic_cell_line
genotype
Ink4a_Arf_KO
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
spleen
sample name
E-MTAB-16288:leukemic_line_12
scientific_name
Mus musculus
strain
Rag2 KO