TKO2 total RNAseq
Mus musculus
Protocols: Cytosolic and chromatin-enriched fractions were obtained by sequential lysis and centrifugation steps giving a cytosolic fraction, followed by nuclei lysis and centrifugation giving a nuclear soluble fraction and a chromatin pellet.  Total RNA was extracted out of nucleosol, chromatin, or total cells by guanidinium thiocyanate-phenol-chloroform extraction followed by proteinase K and Dnase I treatment. Total RNA library preparation was performed by Novogene Co., Ltd, as a lncRNA sequencing service, including lncRNA directional library preparation with rRNA depletion (Ribo-Zero Magnetic Kit)
ENA first public
2026-03-31
INSDC center name
Sorbonne Université - CNRS UMR7238 Computational, Quantitative and Synthetic Biology
INSDC status
public
Submitter Id
E-MTAB-15194:TKO2 total RNAseq
broker name
ArrayExpress
cell line
TKO2
cell type
BMEL
collection date
not collected
common name
house mouse
developmental stage
mouse embryo stage
disease
hepatocellular carcinoma
fraction
total RNA
fraction
total cell
genotype
Triple KO Cbx1-/- Cbx3-/- Cbx5-/-
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
liver
sample name
E-MTAB-15194:TKO2 total RNAseq
scientific_name
Mus musculus