D7 replicate 1 multiplexing seq 3
Homo sapiens
Protocols: Digestion with versene and trippleE. Then cells were labeled with CMO, and sorted for vital cells and then loaded on the 10X controller. We processed the organoids with trypsin and obtained a single-cell suspension. We then labeled the cells with cell multiplexing oligos (CMO) before loading on a reaction of 3' Next GEM single cellv3.1 kit.  Cells were washed with PBS and 1% BSA (Miltenyi) and then labeled with CMOs following the 10x Genomics protocol (CG000391 RevA). After three washes with PBS and 1% BSA, cells were incubated for 15 minutes at room temperature with Fixable Viability Dye eFluor™ 780 (Invitrogen 1:1000 dilution). Cells were washed again and sorted with a SONY SH800S sorter with a 100 µm chip in FACS tubes, then pooled together in equal quantities, and resuspended at 1,600 cells/µL to be loaded on the 10X chromium with a target of 16,000 cells per reaction. 10X genomics technology with CG000388 Rev B (with cell multiplexing oligos). Cells were loaded on a chip G. the single cell library was created with CG000388 Rev B (with cell multiplexing oligos).
ENA first public
2025-10-30
INSDC center name
UniTo
INSDC status
public
Submitter Id
E-MTAB-15711:D7 replicate 1 multiplexing seq 3
broker name
ArrayExpress
cell line
WTC11
collection date
not collected
common name
human
developmental stage
D7 cardioid
disease
normal
genotype
mixed
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
heart left ventricle cardioid
protocol
multiplexing
sample name
E-MTAB-15711:D7 replicate 1 multiplexing seq 3
scientific_name
Homo sapiens