TWIST1 monomer 2
Homo sapiens
Protocols: Human coronary artery smooth muscle cells (HCAMSCs) were ordered from Lonza. HCAMSCs were transduced with lentivirus and prepared for RNA isolation. HCAMSCs were cultured in Lonza's SmBM medium with SmGM-2 Smooth Muscle Cell Growth Medium-2 Bullet Kit, excluding gentamicin sulfate. HCAMSCs were used between passage 5-6 for all experiments.  Once HCAMSCs reached 70% confluence in 6-well plates, serum-free media was applied for 24 hours prior to lentiviral transduction with polybrene. After overnight incubation, the medium was replaced with fresh full-serum SmGM medium. HCASMCs were harvested for RNA isolaion 48 hours later.   FastQ files were processed using nf-core/rnaseq (version 3.17.0) pipeline. Pre-processing to infer strandedness was performed using fq (v0.12.0) and 203 Salmon (v1.10.3).  RNA extraction was performed using the Zymo Quick-RNA Microprep Kit.  Following quality control, the NEBNext Ultra II Directional Kit was used for library preparation.
ENA first public
2025-10-30
INSDC center name
Wirka Lab, UNC Chapel Hill
INSDC status
public
Submitter Id
E-MTAB-15722:TWIST1 monomer 2
broker name
ArrayExpress
cell line
HCAMSC
cell type
smooth muscle cell
collection date
not collected
common name
human
developmental stage
adult
disease
normal
genotype
TWIST1 in pLJM1 backbone
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
coronary artery
sample name
E-MTAB-15722:TWIST1 monomer 2
scientific_name
Homo sapiens