LET-418_int_Rep1
Caenorhabditis elegans
Protocols: C. elegans strains were grown on a lawn of GM48 (-dam) E.coli bacteria on NGM plates at 20°C for at least two generations. After synchronization, around 1500 L1 larvae were seeded on three 100 mm plates. The larvae were grown till the young adult stage and were collected after washing 10-12 times with M9 media in aliquots of 30-50 µl. After the removal of excess liquid, samples (approximately 4500 worms per sample) were snap frozen and stored at -80°C until further use. The list of strains used in the study is mentioned in the supplementary files DNA extraction from frozen worm pellets was performed by using the DNeasy Blood and Tissue Kit (#69504; Qiagen, Valencia, CA).  500 ng of DNA was used for every sample for digestion with dpnI followed by ligation of the adaptors and PCR amplification to generate DNA amplicons of each sample. For the intestinal DamID 18-22 PCR cycles were performed (sup Figure 1B, representative replicate 1). DNA amplicons purifications were performed after every step from the PCR amplification onwards using magnetic beads and DNA quantification was done using QUBIT. Library preparation included end repair, barcoding and pooling of the libraries to be loaded on sequencer.
ENA first public
2025-02-27
INSDC center name
UNIFR
INSDC status
public
Submitter Id
E-MTAB-14824:LET-418_int_Rep1
broker name
ArrayExpress
collection date
not collected
developmental stage
newly molted young adult hermaphrodite
genotype
ljmSi10[hsp-16.2p::loxP::mCherry::let-858 UTR::loxP::let-418 ::Degron::DamMyc::unc-54 3'UTR #9] IV; heSi141[hlh-8p::CRE]X
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
intestine
sample name
E-MTAB-14824:LET-418_int_Rep1
scientific_name
Caenorhabditis elegans
sex
hermaphrodite
strain
CWC42