B23-1_Jej
Gallus gallus
Protocols: Birds (n =12 per strain) were assigned to infection or control groups in a 3 × 2 factorial design. The infected group (n = 18 birds) were inoculated orally with 0.2 mL NaCl with 500 infective eggs of A. galli and H. gallinarum at one week of age while the control group (n = 18 birds) was given a placebo (0.2 mL saline solution). Two weeks after inoculation, birds were killed and tissue samples were dissected, snap frozen in liquid nitrogene and stored at -80 °C. Frozen tissue samples from the jejunum, caecum, and liver (n = 108) were homogenized in liquid nitrogen to extract total RNA. Total RNA was extracted from approximately 40 mg of each sample, with subsequent steps of TRI reagent-based extraction (Sigma-Aldrich, Taufkirchen, Germany), DNaseI treatment (Roche Diagnostics, Mannheim, Germany ), and RNA purification (NucleoSpin RNA kit; Macherey-Nagel, Düren, Germany) according to the manufacturer's instructions.  The RNA concentration was determined through spectrophotometry using the NanoDrop ND-2000 (Peqlab, Erlangen, Germany) and RNA quality of final RNA was analyzed with the Bioanalyzer 2100 device (Agilent Technologies, Waldbronn, Germany).  Total RNA of one microgram (RIN > 8) was used for library preparation using an Illumina Stranded mRNA Prep, Ligation kit with a 10 PCR-cycle of amplification according to the manufacture's recommendation (Illumina). The libraries were quality checked on Bionalyzer for fragment length distribution and normalized to an equal concentration of 10nM prior to pooling.
ENA first public
2025-10-30
INSDC center name
Research Institute for Farm Animal Biology (FBN) Physiological Genomics Unit
INSDC status
public
Submitter Id
E-MTAB-14546:B23-1_Jej
age
3
broker name
ArrayExpress
collection date
not collected
common name
chicken
developmental stage
premature
genotype
wild type genotype
geographic location (country and/or sea)
not collected
infect
control
isolate
not applicable
organism part
jejunum
sample name
E-MTAB-14546:B23-1_Jej
scientific_name
Gallus gallus
sex
male
strain
Lohmann Brown-LB