Sample 8
Arabidopsis thaliana
Protocols: Leaf material was collected from 5 week old plants and frozen in liquid nitrogen following dissection. Leaf discs were cut with a tissue corer from three plants and were pooled as a single biological replicate. Arabidopsis thaliana plants were grown on MS plates for 5 weeks at 10 h light and 14h dark at 22 °C. Leaves were painted with either a mock treatment (water + 0.001% DMSO) or estradiol (20uM + 0.001% DMSO). Leaf tissue was homogenised using a Genogrinder and each Eppendorf tube contained 2 steel ball bearings.  Total RNA was extracted from leaf tissue using the Qiagen Plant RNeasy mini kit according to the manufacturers instructions. Samples were given rigorous treatment with Turbo DNase was undertaken to remove genomic DNA contamination.  Library construction was outsourced to Novogene. Libraries were constructed for paired end reads, using oligo-dT enrichment.
ENA first public
2026-03-31
INSDC center name
John Innes Centre
INSDC status
public
Submitter Id
E-MTAB-16776:Sample 8
age
5
broker name
ArrayExpress
collection date
not collected
common name
thale cress
developmental stage
expanded leaf
genotype
Col-0
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
vascular leaf
sample name
E-MTAB-16776:Sample 8
scientific_name
Arabidopsis thaliana
stimulus
estradiol, 24h
ecotype
Col-0