U2AF1beta/U2AF2alpha-dmso
Homo sapiens
Protocols: After removing growth media, cell monolayers were resuspended in PBS. Cells were centrifuged 1 minute at 100g, and the supernatant was removed prior to RNA extraction. Cells were transfected with expression vectors for either Fritillaria borealis subunits U2AF1 and U2AF2 or beta-galactosidase, then kept during 2 days in culture at 37C in DMEM. Cell cultures were treated with 15 mM Cycloheximide during 8 hours at 37C prior to sample collection. Control samples were treated with DMSO. Total RNA was extracted with Trizol reagent following the manufacturer's protocol and treated with DNase. Total RNA was annealed to a mixture of random primers and Oligo-dT and reverse-transcribed. We used 20 cycles of PCR to amplify cDNA in inidiviual reactions carried out with gene-specific primers for ALG13, eIF1B, FMRI and HSP90B1. The PCR reactions corresponding to the same transfection were processed for native barcoding following the protocol of Oxford Nanopore Technology and pooled together.
ENA first public
2026-06-12
INSDC center name
Michael Sars Centre, University of Bergen
INSDC status
public
Submitter Id
E-MTAB-15870:U2AF1beta/U2AF2alpha-dmso
broker name
ArrayExpress
cell line
HEK293T
collection date
not collected
common name
human
development stage
embryo stage
genetic modification
U2AF1beta/U2AF2alpha
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
kidney
sample name
E-MTAB-15870:U2AF1beta/U2AF2alpha-dmso
scientific_name
Homo sapiens