Dog_gut_microbiome_DMD_D_PM5_230312_Neon3
Canis lupus familiaris
Dog stool sample of Neon
ENA first public
2026-03-31
INSDC center name
UNIVERSITY OF SZEGED
INSDC status
public
Submitter Id
Canfam_stool_DMD_D_PM5_230312_Neon3
adapters
5'-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3'
biological status
breeder's line
broad-scale environmental context
Danube Basin
chimera check software
Kraken2
collection date
2023-03-12
common name
dog
environmental medium
no
geographic location (country and/or sea)
Hungary
geographic location (latitude)
46.181793
geographic location (longitude)
18.954306
geographic location (region and locality)
Baja
gravidity
NA
host age
6.57
host body product
stool
host common name
Hungarian herding terrier: Pumi
host diet
Josera Family Plus and breastmilk
host family relationship
Puppy of Boszi - Litter D
host life stage
puppy
host scientific name
Canis lupus familiaris
host sex
male
host subject id
DMD_D_Neon
host subspecific genetic lineage
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host taxid
9615
library construction method
16S Barcoding Kit (SQK-16S024)
library screening strategy
AMPLICON
library size
1500 bp
local environmental context
garden/house
multiplex identifiers
CCAAACCCAACAACCTAGATAGGC
nucleic acid amplification
LongAmp Taq 2X Master Mix
nucleic acid extraction
Macherey Nagel NucleoSpin DNA Stool kit
pcr conditions
Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
pcr primers
27F 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R 5'-CGGTTACCTTGTTACGACTT-3'
project name
Multiplatform metagenomic sequencing for longitudinal analysis of canine gut microbiome
sample material processing
none
sample name
Canfam_stool_DMD_D_PM5_230312_Neon3
sample storage location
Eppendorf_CryoCube_F740h
sample storage temperature
-80
sample volume or weight for DNA extraction
0,2
scientific_name
Canis lupus familiaris
target gene
16S rRNA
target subfragment
V1-V9
temperature
4