Metagenome-assembled genome: MGYG000051286
Eubacteriales bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome generated from the metagenomic sample ERS1444483 of study ERP019502.
ENA first public
2026-06-12
INSDC center name
EMG
INSDC status
public
Submitter Id
MGYG000051286
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
missing: third party data
binning parameters
missing: third party data
binning software
missing: third party data
broad-scale environmental context
human-associated habitat
broker name
EBI-EMG
collection date
2014-11-06
completeness score
77.2
completeness software
CheckM2_v1.1.0
contamination score
0.54
environmental medium
stool
geographic location (country and/or sea)
Kazakhstan
geographic location (latitude)
51.16050000
geographic location (longitude)
71.47040000
investigation type
metagenome-assembled genome
isolation_source
human gut metagenome
local environmental context
human gut
metagenomic source
human gut metagenome
project name
While population stratification has been extensively studied and quantified in sexually reproducing species, it has been only anecdotally described in others. Here, we conducted a large-scale survey of population stratification in prokaryotic species, sampled from their natural habitat, without isolation or cultivation biases. We surveyed the variation landscape of 71 prevalent microbial species in 2144 human fecal metagenomes, and show that in 44 of these, accounting for 72% of the total assigned microbial abundance, single nucleotide variation can be clustered and stratified into sub-populations (termed subspecies). Individuals are usually dominated by only one such subspecies (per species), as expected of closely related organisms from ecological theory. Geographical dispersal patterns differ between phyla, with Firmicutes subspecies being significantly more geographically restricted. To demonstrate the functional significance of the delineated subspecies, we performed a metagenomic analysis, independent of reference genomes, of the gene content that consistently differs between conspecific subspecies, allowing us to associate groups of genes to properties of the microbial community and to the host. For example, some Eubacterium rectale subspecies specifically harbor an accessory pro-inflammatory flagellum operon that is associated with lower gut community diversity, higher host BMI and higher blood fasting insulin levels. Taken together, subspecies are a natural taxonomic level in prokaryotes and facilitate the functional and ecological understanding of the human gut microbiome in conjunction with its host.
sample derived from
ERS1444483
sample name
MGYG000051286
scientific_name
Eubacteriales bacterium
sequencing method
missing: third party data
taxonomic identity marker
multi-marker approach