UC-10-ACL
Homo sapiens
Protocols: Mucosal pinch biopsies were collected from the ascending colon, transverse colon, descending colon and rectum of patients with either UC or PSC-UC (classified as resembling UC at the clinical level) during routine colonoscopy procedures. Prior to colonoscopy, Picoprep bowel preparation was used, which contains active ingredients sodium picosulfate, magnesium carbonate hydrate, and citric acid. All patients were Australian and of typically white European descent and had undergone no antibiotic use in the 28 days prior to colonoscopy. Inflammatory state was recorded by the treating gastroenterologists during colonoscopy and histologically confirmed by anatomical pathologists with subspecialty interest in gastrointestinal pathology. Mucosal pinch biopsies were immediately transferred to ice-cold RPMI medium containing 10% heat-inactivated (HI) FBS. All procedures were covered by ethics approval from South Eastern Sydney Local Health District Research Ethics Committee (Australian IBD Microbiome (AIM) study; HREC ref no:18/173) or St Vincent's Hospital Human Research Ethics Committee (2024/ETH01125) and followed patient consent.  Mucosal pinch biopsies were thawed, enzymatically digested and washed to generate single cell suspensions. Individual regional samples from 4 donors were pooled equally and loaded into the 10x Genomics Chromium Controller according to the manufacturer's protocol at a concentration that would attain 15,000 cells/chip position. GEX cDNA library preparation was carried out according to the 10x Genomics Chromium Single Cell 5' Reagent Kits User Guide.
ENA first public
2026-06-27
INSDC center name
Garvan
INSDC status
public
Submitter Id
E-MTAB-16401_2:UC-10-ACL
age
23
broker name
ArrayExpress
cell type
colon
collection date
not collected
common name
human
developmental stage
adult
disease
ulcerative colitis
geographic location (country and/or sea)
not collected
individual
UC-10
inflammation
noninflamed
isolate
not applicable
organism part
ascending colon
sample name
E-MTAB-16401_2:UC-10-ACL
scientific_name
Homo sapiens
sex
male