shFOXO3_500nM_DOX_1_S39
Homo sapiens
Protocols: After the exposure, cells were washed once with PBS and subsequently lysed in 0.5 mL QIAzol Lysis Reagent (79306, Qiagen). Total RNA was extracted using chloroform and isopropanol precipitation. Poly(A) libraries were generated using the Zephyr G3® NGS automated system with the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 (NOVA-5198–02, PerkinElmer). Library preparation included NEXTFLEX® Poly(A) Beads 2.0 (NOVA-512992, PerkinElmer) for mRNA enrichment and NEXTFLEX® Unique Dual Index Barcodes (NOVA-512923, PerkinElmer) for sample multiplexing. Each library was prepared using 100 ng of total RNA as input, with RNA integrity numbers (RIN ≥ 8). A total of 13 PCR cycles were performed during library amplification.
ENA first public
2025-12-29
INSDC center name
Maastricht University
INSDC status
public
Submitter Id
E-MTAB-16022:shFOXO3_500nM_DOX_1_S39
broker name
ArrayExpress
cell line
shFOXO3
cell type
cardiomyocytes
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-16022:shFOXO3_500nM_DOX_1_S39
scientific_name
Homo sapiens