Sample 11
Homo sapiens
Protocols: Using a neuro-navigation system with pre-surgical MRI, ten patients were resected from IDHwt glioblastoma with tumor sampling both in the tumor core (T1-gadolinium positive area) and the tumor periphery (T2 FLAIR positive but T1-gadolinium negative area) Total RNA was isolated from 10 paired glioblastoma samples (10 core and 10 periphery samples). Samples were homogenized with a TissueLyser (Qiagen, Hilden, Germany) and loaded on a Maxwell® RSC Instruments (Promega, Madison, WI, USA) for automated RNA extraction with the Maxwell® RSC miRNA Tissue Kit, according to the manufacturer's instructions.  Libraries were prepared starting from 200 ng of total RNA with the TruSeq Stranded Total RNA Library Preparation kit (Illumina, San Diego, CA, USA), following the manufacturer's instruction.
ENA first public
2026-06-12
INSDC center name
Humanitas University
INSDC status
public
Submitter Id
E-MTAB-15143:Sample 11
age
44
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
brain glioblastoma
genotype
IDHwt
geographic location (country and/or sea)
not collected
individual
2
isolate
not applicable
organism part
brain
sample name
E-MTAB-15143:Sample 11
sampling site
tumor core (C)
scientific_name
Homo sapiens
sex
male