Sample 1
Mus musculus
Protocols: We sorted live, CD45+ cells from single-cell suspensions of tumors from 3 mice using a FACS (BD Biosciences).  The single-cell suspension was subjected to quality control with the following criteria: cell viability must meet the requirements (>80% by AO/PI fluorescent staining or >75% by trypan blue exclusion), cell concentration between 700-1,200 cells/μL, cell diameter ranging from 5 to 30 μm, total cell count up to 100,000, clean suspension background with no significant debris or impurities. After quality control, the viable cells were washed, resuspended, and prepared as a single-cell suspension at an appropriate concentration. The cell suspension was then loaded according to the target cell recovery count. Following loading, the formation of GEMs (Gel Bead-In-Emulsions) was monitored. If GEMs formed successfully, they were transferred to a PCR tube for subsequent reverse transcription and library construction. Once the constructed library passed quality control, it was subjected to sequencing.
ENA first public
2025-10-30
INSDC center name
ZJU
INSDC status
public
Submitter Id
E-MTAB-15770:Sample 1
broker name
ArrayExpress
cell type
CD45+
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
MG53 ko fl mice
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
anatomy basic component
sample name
E-MTAB-15770:Sample 1
scientific_name
Mus musculus