58
Homo sapiens
Protocols: Peripheral blood (PB) was collected from healthy donors, patients with autoimmune disease, and patients with diffuse large B-cell lymphoma (DLBCL) following written informed consent and institutional review board approval. Blood was drawn into EDTA anticoagulant tubes and processed within 4 hours of collection. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque density gradient centrifugation according to the manufacturer's instructions. PBMCs were washed twice in PBS and either processed immediately for cell enrichment and flow cytometric sorting or cryopreserved in fetal bovine serum supplemented with 10% DMSO and stored in liquid nitrogen until further use. Genomic DNA was extracted from sorted Aged B cells, Memory B cells, and CD19⁻ fractions using the QIAamp DNA Mini Kit (Qiagen) according to the manufacturer's protocol. DNA from diagnostic tumor biopsies (formalin-fixed paraffin-embedded tissue) was extracted using the MagMAX™ FFPE DNA/RNA Ultra Kit (Thermo Fisher Scientific). DNA concentration was measured using Qubit fluorometry, and samples were normalized to ~50 ng input per library preparation when possible. When DNA yield was limiting, samples were concentrated using DNA Clean & Concentrator columns (Zymo Research). Targeted DNA libraries were prepared using a custom molecular inversion probe (MIP) panel targeting 91 genes recurrently mutated in diffuse large B-cell lymphoma. Libraries were generated according to the manufacturer's protocol (Sequentify Ltd.) with unique molecular identifiers (UMIs) incorporated to enable molecular deduplication and error correction. For each sample, two independent technical replicate libraries were generated from the same genomic DNA input. Libraries were amplified by PCR, purified using AMPure XP beads, and quantified prior to sequencing. For immunoglobulin heavy-chain (IGH) repertoire analysis, targeted amplification of V(D)J junctions was performed using a dedicated IGH sequencing assay according to the manufacturer's protocol.
ENA first public
2026-06-27
INSDC center name
Wizmann Institute of science
INSDC status
public
Submitter Id
E-MTAB-17140:58
age
83
broker name
ArrayExpress
cell type
mononuclear cell
collection date
not collected
common name
human
developmental stage
adult
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
G51
isolate
not applicable
organism part
blood
sample name
E-MTAB-17140:58
scientific_name
Homo sapiens
sex
female