Negative blank control from 16S lab processing
metagenome
metabarcoding of DNA from negative control
plant body site
NA
source material identifiers
NA
broad-scale environmental context
laboratory environment
host scientific name
NA
collection date
2023-06-01
target gene
16S;ITS2
host diameter at breast height
NA
geographic location (longitude)
10.40399870
host common name
NA
taxonomic classification
NA
nucleic acid extraction
KingFisher Tissue kit, ThermoFisher Scientific
experimental factor
NA
geographic location (country and/or sea)
Norway
organism
metagenome
host taxid
256318
environmental medium
sterile water
geographic location (latitude)
63.4140716
scientific_name
metagenome
sample collection method
tube of water/buffer
target subfragment
16Sv4;ITS2
geographic location (region and locality)
NA
pcr primers
16S-515F:GTGYCAGCMGCCGCGGTAA;16S-806R:GGACTACNVGGGTWTCTAAT;ITS-Cha3:CAACTCTCRRCAACGGATA;ITS-u4:RGTTTCTTTTCCTCCGCTTA
local environmental context
laboratory environment
project name
Birch tree bark microbiota
sampling height
NA
project accession
PRJEB109031
sampling aspect on trunk
NA