ARHI0A
Homo sapiens
Protocols: Nuclei isolation was performed using published Omni-ATAC protocol (Corces et al. 2017) with the following modification: cell centrifugation was performed with 700 g for 5 min.  For androgen induction studies, RWPE-1 cells were grown in phenol red-free RPMI-1640 medium with 5% charcoal/dextran-treated (CCS) FBS and 1% glutamine for 3 days. LNCaP were grown in same medium but with 10% charcoal/dextran-treated(CCS) FBS. For androgen induction studies, hormone-deprived cells were treated with 0, 1, 10 or 100 nM of dihydrotestosterone (DHT) for 2 hours before nuclei isolation. Does not apply to ATAC-sequencing. See published Omni-ATAC protocol (Corces et al. 2017) ATAC-library preparation was performed using published Omni-ATAC protocol (Corces et al. 2017) with the following modifications: For Tagmentation reaction conditions were adjusted to 45 min in 37 °C at 900 RPM. And for the PCR purification steps, MinElute kit (Qiagen) was used and the library purification was finalized using AMPure Xp beads (Beckman Coulter).
ENA first public
2026-03-31
INSDC center name
Faculty of Medicine and Health Technology, Tampere University and Tays Cancer Centre, Tampere University Hospital, Tampere, Finland
INSDC status
public
Submitter Id
E-MTAB-15731:ARHI0A
broker name
ArrayExpress
cell line
LNCaP-ARhi
cell type
prostate gland cancer cell
collection date
not collected
common name
human
developmental stage
adult
disease
prostate cancer
genetic modification
pcDNA3.1-AR
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
prostate gland
replicate
1
sample name
E-MTAB-15731:ARHI0A
scientific_name
Homo sapiens
stimulus
0_nM_DHT