dog_stool_microbial_sample_Narancs6
Canis lupus familiaris
Dog stool sample of Narancs
ENA first public
2026-03-31
INSDC center name
UNIVERSITY OF SZEGED
INSDC status
public
Submitter Id
Canfam_stool_DMD_D_PF1_230320_WGS_Narancs6
adapters
CTGTCTCTTATACACATCT
broad-scale environmental context
Danube Basin
collection date
2023-03-20
common name
dog
environmental medium
no
geographic location (country and/or sea)
Hungary
geographic location (latitude)
46.181793
geographic location (longitude)
18.954306
geographic location (region and locality)
Baja
host age
7.71
host body product
stool
host common name
Hungarian herding terrier: Pumi
host diet
Josera Family Plus + breastmilk
host family relationship
Puppy of Boszi - Litter D
host life stage
puppy
host scientific name
Canis lupus familiaris
host sex
female
host subject id
not applicable
host subspecific genetic lineage
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host taxid
9615
library construction method
Illumina DNA Prep Kit (Document #1000000025416v10)
library screening strategy
WGS
library size
300 bp
local environmental context
garden/house
multiplex identifiers
GGACTCCT / CGTCTAAT
nucleic acid amplification
Enhanced PCR Mix (EPM)
nucleic acid extraction
Macherey Nagel NucleoSpin DNA Stool kit
pcr conditions
3 min 68 �C (1 cycle); 3 min 98 �C (1 cycle); 45 secs 98 �C (5 cycles); 30 secs 62 �C (5 cycles); 2 mins 68 �C (5 cycles); 1 min 68 �C (1 cycle); Hold at 10 �C
pcr primers
Index 1 Read: 5? CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGG; Index 2 Read: 5? AATGATACGGCGACCACCGAGATCTACAC[i5]TCGTCGGCAGCGTC
project name
Multiplatform metagenomic sequencing for longitudinal analysis of canine gut microbiome
sample name
Canfam_stool_DMD_D_PF1_230320_WGS_Narancs6
sample storage location
Eppendorf_CryoCube_F740h
sample storage temperature
-80
sample volume or weight for DNA extraction
0,2
scientific_name
Canis lupus familiaris
target gene
whole genom
target subfragment
whole genom
temperature
11