J_d_t
soil metagenome
alpine-permafrost affected soil
ENA first public
2026-04-16
INSDC center name
EPFL
INSDC status
public
Submitter Id
J_d_t
altitude
2652.6
broad-scale environmental context
mountain biome [ENVO:01000364]
collection date
2023-06-20
current land use
recreational area
current vegetation
Scree and rubble communities
current vegetation method
Habitat Map of Switzerland (Federal Office for the Environment FOEN), Delarze et al., 2082
depth
50
elevation
2652.6
environmental medium
soil
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.48664
geographic location (longitude)
8.06843
geographic location (region and locality)
Grächen, Valai, Switzerland
local environmental context
permafrost soil environment or alpine scree
negative control type
not applicable
nucleic acid extraction
DNeasy PowerSoil Pro Kits for Soil DNA Extraction
pcr primers
bacterial 16S rRNA 22f primer 5'-AGA GTT TGA TCM TGG CTC AG-3'; 1392r primer 5'-CGG TTA CCT TGT TAC GAC TT-3'
project name
Vegetation extent shapes bacterial community structure
sample collection device
shovel
sample collection method
Surface soil (0–15 cm) manually collected using a sterilized shovel and transferred to sterile sampling containers.
sample name
J_d_t
sample storage conditions
frozen at -20°C for 9 months
sample volume or weight for DNA extraction
1
scientific_name
soil metagenome