GEX_163_postInfusion_CD3+
Homo sapiens
Protocols: Frozen human BM and Infusion Product (IP) cryotubes were rapidly thawed and stained with hCD45 PO (clone HI30, Invitrogen) and CD3 PerCP (clone SK7, BD) or with CD19 biotin protein (Miltenyi) followed by Biotin APC (REA746, Miltenyi) and CD3 PercP (clone SK7, BD) antibodies, respectively. BM cells were separated into CD45+CD3+ and CD45+CD3- fractions, whereas IP was separated into CD3+CAR+ and CD3+CAR- cells by flow cytometry-based sorting (BD FACS ARIA III analyser). All samples were gated based on physical parameters, followed by the exclusion of doublets and death cells (DAPI high). After sorting, cells were counted and checked for viability For each sample,cells were loaded in a 10x Genomics Chromium Next GEM Chip according to manusfacturer's protocol Libraries were constructed using 10x Genomics Chromium Next GEM Single Cell 5' v1 kit
ENA first public
2025-12-31
INSDC center name
Tettamanti Center, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy
INSDC status
public
Submitter Id
E-MTAB-14549:GEX_163_postInfusion_CD3+
age
2
broker name
ArrayExpress
cell_type
T cell
collection date
not collected
common name
human
developmental stage
infant
disease
B-cell acute lymphoblastic leukemia
geographic location (country and/or sea)
not collected
individual
1
infusion product
FT01 CARCIK-CD19
isolate
not applicable
organism part
bone marrow
sample name
E-MTAB-14549:GEX_163_postInfusion_CD3+
scientific_name
Homo sapiens
sex
female