Combo 1 KMS-28PE
Homo sapiens
Protocols: INA-6, OPM2 and KMS-28PE were selected for combinatorial treatment response analysis. INA-6 and KMS28-PE cells were seeded at 100.000 cells/ml while OPM2 cells were seeded at 200.000 cells/mL 24h prior treatment with H2O and DMSO (Sigma-Aldrich) or 50nM of AZA (Abcam) and 5μM or 10μM of A366 (Tocris Bio-techne). AZA treatment was administrated every day while A366 treatment was added every 3 days. Media and reagent refreshment was performed every 3 days. All experiments were performed in three independent cell batches per cell line. RNA was extracted using the RNeasy Micro Kit (Qiagen; Hilden; Germany #74004) as described by the manufacturer. Sequencing libraries were prepared from 100ng total RNA using the Illumina Stranded Total RNA library preparation kit with Ribo-Zero Plus treatment (cat# 20040525/20040529, Illumina Inc.)
ENA first public
2025-12-31
INSDC center name
Department of Immunology, Genetics and Pathology, Cancer Precision Medicine
INSDC status
public
Submitter Id
E-MTAB-13813:Combo 1 KMS-28PE
broker name
ArrayExpress
cell_line
KMS-28PE
cell_type
plasma cell
collection date
not collected
common name
human
disease
multiple myeloma
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-13813:Combo 1 KMS-28PE
scientific_name
Homo sapiens
stimulus
A366 10 uM + Aza 200 nM