Glycyrrhiza glabra
ENA first public
2026-02-09
INSDC status
public
age
not collected
air_temperature_regimen
not collected
biological_material_ID
GLY8_7
biological_material_altitude
112m
biological_material_coordinates_uncertainty
2000m
biological_material_geographic_location
Germany
biological_material_latitude
51.816083
biological_material_longitude
11.283083
biological_material_ploidy
diploid
checklist
ERC000011
collection date
1954
genus
Glycyrrhiza
geographic location (country and/or sea)
not collected
growth_facility
not collected
library_type
GBS
light_regimen
not collected
material_source_altitude
not collected
material_source_description
not collected
material_source_geographic_location
not collected
material_source_latitude
not collected
material_source_longitude
not collected
plant_anatomical_entity
leaf
plant_structure_development_stage
not collected
project_name
Glycyrrhiza study
sample name
GLY8_7
sample_description
For the panel of genebank accessions, genomic DNA from fresh leaves was extracted with the DNeasy Plant mini kit (Qiagen, Hilden, Germany) following the manufacturer's protocol. The DNA from the Iranian populations was isolated from fresh leaves with the CTAB procedure as described by Doyle and Doyle (1987). A Nanodrop spectrophotometer (A260/280, ND-1000 PEQLAB Biotechnologie GmbH) was used to verify the purity of the DNA, and an 1.3 % agarose gel was used to control the integrity of the DNA. In total, 200 ng DNA per sample were digested with PstI and MspI (New England Biolabs) and processed for GBS library construction as described by Zhang et al. 2024. Equimolar pools of the individually barcoded samples (unique dual indexes) were sequenced were sequenced (Illumina Novaseq 6000 device, single read, 107 cycles) using a custom sequencing primer (Wendler et al. 2014) according to the manufacturer's instructions (Illumina Inc., San Diego, CA, United States).
sample_id
IPK_LIMS:2930216
sample_title
GLY8_7
sequencing_date_ipk
2022-07-14
species
glabra