DBL-11
Homo sapiens
Protocols: Blood was collected in EDTA tubes from subjects, and peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation with SepMate-50 tubes (Stemcell Technologies) according to manufacturer's instructions. Frozen PBMCs were thawed and cells were counted using Nexcellom Cellometer Spectrum, and cell viability assessed using Live/DEAD Viability/Cytotoxicity Kit for mammalian cells (Thermo Scientific). Cells are then partitioned into nanoliter-scale gel beads-in-emulsion (GEMs). Gel beads are dissolved, primers are released and any co-partitioned cell is lysed. Incubation of GEMs generates barcoded, full length cDNA from poly-adenylated mRNA. Single cell library preparation was performed using 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) by Molecular Genomics Pte Ltd. Profiles of cDNA samples and the final libraries were verified using Agilent High Sensitivity D5000 screentapes on an Agilent Tapestation 4200. At least 5000 cells were targeted per sample with at least 20,000 reads sequenced per cell.
ENA first public
2025-12-31
External Id
SAMEA114503800
INSDC center name
Viral Research and Experimental Medicine Centre, SingHealth Duke-NUS Academic Medical Centre, Singapore
INSDC last update
2023-10-17T10:53:55Z
INSDC status
public
Submitter Id
E-MTAB-13441:DBL-11
age
24
broker name
ArrayExpress
cell_type
mononuclear cell
clinical history
Dengue seronegative
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
individual
DBL-11
isolate
not applicable
organism part
blood
sample name
E-MTAB-13441:DBL-11
scientific_name
Homo sapiens
sex
male