Pseudomonas putida
Strain was cultured overnight in LB medium, washed and resuspended. Baffled flasks containing 25 mL M9 minimal medium + 30 mM PCA were inoculated to an initial OD600nm of 0.1. Flasks were incubated at 225 rpm and 30C. When cultures reached mid-exponential phase (OD600nm ~1.0-1.5, between 12 and 24 h), 2 mL of cells were pelleted at 6000 xg for 1 min, flash frozen on liquid nitrogen, and frozen at -80C.
strain
AW71
isolate
AW71-B
isolation_source
Engineered strain
collection_date
2023-12-15
geo_loc_name
USA
sample_type
Cell culture
biomaterial_provider
National Laboratory of the Rockies
collected_by
Tracy Hodges, Alissa Bleem
genotype
catRBC::Ptac:catA1 pcaHG::Ptac:aroY:ecdBD fpvA:Ptac:praI:vanAB pobAR crc::Ptac:catB:catC
samp_size
2 mL
temp
30 C
BioSampleModel
Microbe, viral or environmental