Sample 1
Homo sapiens
Protocols: Peripheral blood samples were collected from 6 healthy volunteers (Con group), 7 patients meeting the Berlin Definition of ARDS at the Intensive Care Units of Xishan People's Hospital of Wuxi City and Affiliated Hospital of Jiangnan University between January 2024 and December 2024.  Total RNA was extracted from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis. RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) and sequenced on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 150-bp paired-end reads.  Total RNA was extracted from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis. RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) and sequenced on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 150-bp paired-end reads.
ENA first public
2026-07-03
INSDC center name
Department of Intensive Care Unit, Xishan People's Hospital of Wuxi City (Wuxi Branch of Zhongda Hospital Southeast University), Wuxi 214105, China
INSDC status
public
Submitter Id
E-MTAB-17303:Sample 1
broker name
ArrayExpress
collection date
not collected
common name
human
disease
ARDS
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
PBMC
sample name
E-MTAB-17303:Sample 1
scientific_name
Homo sapiens