H_e_t
soil metagenome
alpine-permafrost affected soil
ENA first public
2026-04-16
INSDC center name
EPFL
INSDC status
public
Submitter Id
H_e_t
altitude
2484.3
broad-scale environmental context
mountain biome [ENVO:01000364]
collection date
2023-06-23
current land use
recreational area
current vegetation
Scree and rubble communities
current vegetation method
Habitat Map of Switzerland (Federal Office for the Environment FOEN), Delarze et al., 2074
depth
50
elevation
2484.3
environmental medium
soil
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.091333
geographic location (longitude)
7.59537
geographic location (region and locality)
Cabane de Diableretz, Valais, Switzerland
local environmental context
permafrost soil environment or alpine scree
negative control type
not applicable
nucleic acid extraction
DNeasy PowerSoil Pro Kits for Soil DNA Extraction
pH
6.85
pH method
2 g of frozen soil were mixed with 5 mL 0.05 M CaCl2 solution and pH was measured with a Orion Lab Star PH111 pH/mV meter (Thermo Fisher Scientific, WA, USA)
project name
Vegetation shapes European alpine permafrost microbiome
sample collection device
shovel
sample collection method
Surface soil (0–15 cm) manually collected using a sterilized shovel and transferred to sterile sampling containers.
sample name
H_e_t
sample storage conditions
frozen at -20°C for 9 months
sample volume or weight for DNA extraction
1
scientific_name
soil metagenome
temperature
13.3
total nitrogen content
12817.115
total nitrogen content method
TN measured on dried and ground soil using a Thermo Finnigan Flash EA 112 CHSN elemental analyzer.
total organic carbon
278.8881169
total organic carbon method
TOC measured on acidified soil (HCl removal of inorganic carbon) using a Thermo Finnigan Flash EA 112 CHSN elemental analyzer.
water content
27.46282528
water content method
Gravimetric water content was determined by drying 2 g of frozen soil at 100 °C for 24 h