Lysinibacillus pinottii
OYA S33, isolated from the rhizosphere soil of pepper (Capsicum annuum) is a candidate plant growth-promoting rhizobacterium (PGPR). Wet laboratory analysis and field trial for the organism shows a plant-enhancing traits such as production of the phytohormone indole-3-acetic acid, solubilization of inorganic phosphate, and biocontrol activity against pathogens, consistent with previous reports of members of the genus Lysinibacillus. These functional traits make Lysinibacillus spp. valuable for sustainable agriculture as biofertilizers and biocontrol agents. The draft genome of Lysinibacillus sphaericus str. OYA S33 is 4,397,871 bp in length, assembled into 28 contigs with an N50 of 975,684 bp and a GC content of 37.3%. Genome annotation predicted 4,502 protein-coding sequences, 4 rRNA genes, and 65 tRNA genes. No plasmids or additional replicons were detected. The assembly and annotation were generated using Unicycler v0.4.8 and the RAST tool kit (RASTtk) pipelines. The genome enables identification of genetic determinants underlying plant growth-promoting traits and provides a resource for comparative genomics and development of this isolate as a PGPR candidate.
strain
OYA S33
chem_administration
NA
collection_date
2024-05-20
env_broad_scale
terrestrial biome
env_local_scale
agricultural soil
env_medium
rhizosphere soil
food_source
NA
geo_loc_name
Nigeria: Ekiti state, Oye-Ekiti
host_age
10 weeks
host_common_name
pepper
host_genotype
NA
host_height
50cm
host_length
NA
host_life_stage
Vegetative
host_phenotype
NA
host_tot_mass
NA
isol_growth_condt
https://www.scirp.org/journal/paperinformation?paperid=89746
lat_lon
7.776695 N 5.312083 E
microbial_biomass
NA
microbial_biomass_meth
NA
num_replicons
1
ref_biomaterial
This study; isolate obtained from rhizosphere soil of pepper (Capsicum annuum)
samp_store_dur
24 HOURS
samp_store_loc
Laboratory Freezer
sieving
2mm sieve
soil_type
Loamy soil
soil_type_meth
Field observation
store_cond
-10C
temp
30C
isolation_source
Rhizosphere soil of pepper (Capsicum annuum)
neg_cont_type
Sterile NA medium
pos_cont_type
Bacillus subtilis as growth control.
Incubation Temperature
30C
Isolation Medium
Nutrient Agar
Cultural Morphology
circular, raised elevation, entire margin, cream color and smooth surface.
BioSampleModel
MIGS.ba
BioSampleModel
MIGS/MIMS/MIMARKS.agriculture