Sample 55
Mus musculus
Protocols: RNA was isolated from the epiphysis of one femur per mouse that was homogenized in TRIzol® (n=3 for each of the following mouse genotypes: SHIPstyx/styx, SHIP1+/styx, wild-type (C57BL6/J), Rag2-/-/Il2rg-/-/SHIP1styx/styx, Rag2-/-/Il2rg-/-/SHIP1+styx, and Rag2-/-/Il2rg-/-/SHIP1+/+). Alternatively, total RNA was extracted using the NucleoSpin® RNA Plus Kit . RNA was isolated according to the manufacturer's instructions and RNA concentration and integrity were assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit RNA BR Assay Kit (Thermo Fisher Scientific, Q10211) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer RNA Kit (Agilent, DNF-471), respectively.  Sequencing libraries were made with 1000 ng input RNA using an illumina TruSeq Stranded mRNA Library Prep kit (illumina, 20020595) in combination with TruSeq RNA UD Indexes (illumina, 20022371) according to illumina's guidelines.
ENA first public
2025-12-31
INSDC center name
AO Research Institute Davos, Davos, Switzerland
INSDC status
public
Submitter Id
E-MTAB-14418:Sample 55
age
9
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
mouse postnatal
genotype
Rag2-/-/Il2rg-/-/ SHIP1styx/styx
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Epiphysis of femur
sample name
E-MTAB-14418:Sample 55
scientific_name
Mus musculus
sex
female
strain
C57BL6/J