HC2
Homo sapiens
Protocols: Peripheral blood was collected from rheumatoid arthritis (RA) patients and age-, sex-, and ethnicity-matched healthy controls via venipuncture. For RA patients, blood samples were collected longitudinally over a 12-week period at specific clinical intervals (including baseline, 3 weeks, 6 weeks and 12 weeks post-treatment initiation). Peripheral blood mononuclear cells (PBMCs) were immediately isolated from whole blood using density gradient centrifugation (e.g., Ficoll-Paque), washed, and cryopreserved in liquid nitrogen until further processing to minimize batch effects. Not applicable. This study profiles primary human immune cells isolated directly from patient peripheral blood which were not subjected to ex vivo expansion or culturing prior to downstream assays. Healthy control samples received no therapeutic interventions. For the longitudinal patient cohorts, individuals were treated clinically with standard first-line Methotrexate (MTX) therapy. PBMCs collected at subsequent time points (Weeks 3, 6 and 12) represent the in vivo effects of continuous MTX exposure on peripheral immune cell dynamics relative to the untreated baseline (Week 0). Single-cell RNA-seq libraries were constructed from individual healthy control PBMC samples using the Chromium Controller and the 10x Genomics Next GEM Single Cell 3' Reagent Kit (v3/v3.1) via cDNA fragmentation, adapter ligation, and sample index PCR amplification according to the manufacturer's instructions.
ENA first public
2026-07-02
INSDC center name
Division of Rheumatology, Medical University of Vienna
INSDC status
public
Submitter Id
E-MTAB-17153:HC2
broker name
ArrayExpress
cell type
peripheral blood mononuclear cells
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blood
sample name
E-MTAB-17153:HC2
scientific_name
Homo sapiens