Sample 48
Homo sapiens
Protocols: Metachronous colorectal liver metastasis tissue samples and their adjacent non-malignant liver tissues surgically resected from patients.  Total RNA was extracted from tumor tissue using the TRIZOL reagent (Thermofisher) following the manufacturer's protocol. The tissue was first pulverized with mortar and pestle under liquid nitrogen.  1000 ng of total RNA was used for construction of small RNA libraries. The libraries were prepared using NEXTflex small RNA-Seq Kit v4 (PerkinElmer, Inc, Austin, USA), following the manufacturer's protocol. Quality of prepared libraries was checked by Tapestation 2200 using High Sensitivity D1000 kit (Agilent Technologies Inc., CA, USA) and quantity measured by the Qubit instrument using the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific, MA, USA). Prepared libraries were pooled equimolarly.
ENA first public
2026-01-14
INSDC center name
Toxicogenomics Unit, National Institute of Public Health
INSDC status
public
Submitter Id
E-MTAB-15020:Sample 48
age
56
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
metastatic colorectal cancer
geographic location (country and/or sea)
not collected
individual
8048Z
isolate
not applicable
organism part
liver
sample name
E-MTAB-15020:Sample 48
sampling site
liver
scientific_name
Homo sapiens
sex
male