scc25-nf_nf_03
Heterocephalus glaber
Protocols: For two-dimensional co-culture, SCC25-tdTomato cells were seeded in a 1:1 ratio with mouse or naked mole-rat fibroblasts in DMEM/F12 medium (11320-032, Life Technologies) supplemented with 0.05% FBS (Life Technologies), 400 ng/ml hydrocortisone (Santa Cruz Biotechnology) and 1% Penicillin-Streptomycin (PEST) and cultured in a 37°C incubator with 5% CO2. After 48 hours SCC25-tdTomato and fibroblasts were separated using FACS sorting (E-cadherin-APC staining to separate SCC25 from fibroblasts (TdTom &E-cadherin-). Approximately 50,000-100,000 cells were sorted of each cell type from each condition. Total RNA was extracted by adding 800 µl TRIzol reagent (Life Technologies). Total RNA samples were DNased treated using Turbo DNase (Life Technologies). RNA-sequencing libraries prepared using Illumina stranded total RNA Prep, Ligation with RiboZero Plus (Illimuna) following manufacturer's instructions.
ENA first public
2025-12-31
External Id
SAMEA114624343
INSDC center name
German Cancer Research Center (DKFZ)
INSDC last update
2023-11-15T20:28:45Z
INSDC status
public
Submitter Id
E-MTAB-13538:scc25-nf_nf_03
broker name
ArrayExpress
cell_line
Ispr07
cell_type
fibroblast
collection date
not collected
common name
naked mole-rat
developmental stage
not available
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
growth condition
Naked mole-rat fibroblasts co-cultured with SCC25 cells
isolate
not applicable
organism part
not available
sample name
E-MTAB-13538:scc25-nf_nf_03
scientific_name
Heterocephalus glaber