TWIST1-E47 1
Homo sapiens
Protocols: Human coronary artery smooth muscle cells (HCAMSCs) were ordered from Lonza. HCAMSCs were transduced with lentivirus and prepared for RNA isolation. HCAMSCs were cultured in Lonza's SmBM medium with SmGM-2 Smooth Muscle Cell Growth Medium-2 Bullet Kit, excluding gentamicin sulfate. HCAMSCs were used between passage 5-6 for all experiments.  Once HCAMSCs reached 70% confluence in 6-well plates, serum-free media was applied for 24 hours prior to lentiviral transduction with polybrene. After overnight incubation, the medium was replaced with fresh full-serum SmGM medium. HCASMCs were harvested for RNA isolaion 48 hours later.   FastQ files were processed using nf-core/rnaseq (version 3.17.0) pipeline. Pre-processing to infer strandedness was performed using fq (v0.12.0) and 203 Salmon (v1.10.3).  RNA extraction was performed using the Zymo Quick-RNA Microprep Kit.  Following quality control, the NEBNext Ultra II Directional Kit was used for library preparation.
ENA first public
2025-10-30
INSDC center name
Wirka Lab, UNC Chapel Hill
INSDC status
public
Submitter Id
E-MTAB-15722:TWIST1-E47 1
broker name
ArrayExpress
cell line
HCAMSC
cell type
smooth muscle cell
collection date
not collected
common name
human
developmental stage
adult
disease
normal
genotype
TWIST1-E47 heterodimer
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
coronary artery
sample name
E-MTAB-15722:TWIST1-E47 1
scientific_name
Homo sapiens