Isolated Human Hepatocytes, 1, PNPLA3-I148M
Homo sapiens
Protocols: total RNA was extracted from isolated primary human hepatocytes using RNeasy Plus Micro Kit (Qiagen).  RNA integrity was assessed using the High Sensitivity RNA ScreenTape system on an Agilent 2200 TapeStation (Agilent). The SMART-Seq HT Kit (Takara Bio) was used to generate cDNA from 10 ng of total RNA, and the cDNA product was checked by an Agilent Fragment Analyzer system (Agilent) for quality control. The sequencing library was constructed by following the Illumina Nextera XT Sample Preparation Guide.
ENA first public
2025-10-30
INSDC center name
UMICH
INSDC status
public
Submitter Id
E-MTAB-15689:Isolated Human Hepatocytes, 1, PNPLA3-I148M
broker name
ArrayExpress
cell type
hepatocyte
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
liver
response to treatment
I148M
sample name
E-MTAB-15689:Isolated Human Hepatocytes, 1, PNPLA3-I148M
scientific_name
Homo sapiens