WT-O-2
Mus musculus
Protocols: Harvested knees joint were dissected using surgical scissors, snap frozen immediately, and then stored in liquid nitrogen until the extraction step. Protocol for young mice: The induction of osteoarthritis will be performed by injecting 5 μl of type VII collagenase (1U/5μl) into the joint of one of the mouse's knees on day 0 and day 2 (the other knee being injected with physiological saline solution). The mice will be anesthetized with isoflurane, and the leg will be shaved at the knee joint. A small incision (2mm) of the skin will be made at the central part of the joint. The needle of a 10μl Hamilton syringe will be inserted at the center of the patellar tendon to reach the intra-articular space, and the collagenase solution (or the  physiological saline solution for the Sham knee joint) will be injected. The wound is sterilized with betadine. CiOA mice were euthanized at day 42 after the first collagenase injection. Protocol for old mice: Osteoarthritis in older mice is spontaneous age-related. Therefore, the mice underwent no interventions. They were simply euthanized at 18 months.  The grinding of the whole joint was performed using a pestle and mortar in liquid nitrogen. Total RNA from the resulting powder tissue was extracted using TRI Reagent (Sigma Aldrich #T94424) and RNeasy Kit (QIAGEN) according to the manufacturer's instructions. The quantity and quality of extracted RNA was evaluated using the NanoDrop ND-1000 spectrophotometer and the Agilent 2100.  Nucleic acid library construction protocol was performed according to the manufacturer's instructions by the Beijing Genomic Institute (BGI).
ENA first public
2025-12-31
INSDC center name
IRMB - Institute for Regenerative Medicine & Biotherapy - INSERM U1183
INSDC status
public
Submitter Id
E-MTAB-14217:WT-O-2
age
21
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
p21+/+
geographic location (country and/or sea)
not collected
individual
WT-old
infect
Old WT
isolate
not applicable
organism part
knee joint
sample name
E-MTAB-14217:WT-O-2
scientific_name
Mus musculus
sex
male
strain
C57BL/6N