siCDIPTOSP_2
Homo sapiens
Protocols: OC cell lines (SKOV3, A2780, OVCAR3, HO8910 and CaoV3) and a normal ovarian epithelial cell line (IOSE80) were obtained from the Cell Bank of Chinese Academy of Sciences (Shanghai, China), and cultured in a humidified incubator at 37°C with 5% CO2. RPMI-1640 medium (Gibco, USA) supplemented with 1% penicillin/streptomycin (PS) (NCM Biotech, China) and 10% fetal bovine serum (FSP500, ExCell Bio, China) was used to culture IOSE80, SKOV3, A2780,HO8910 and CaoV3 cells. OVCAR3 cells were cultured in RPMI-1640 medium containing 20% fetal bovine serum. SiRNAs (Gene Pharma, Shanghai, China) targeting CDIPTOSP,STAU1, and a negative control (si-NC) were transfected into SKOV3 and A2780 cells using Lipofectamine 2000 (Invitrogen, USA). Total RNA was extracted from cells transfected for 48 hours using TRIzol reagent (Vazyme, Nanjing, China). Subsequently, reverse transcription was performed using the HiScript III RT SuperMix for qPCR kit (Vazyme) in accordance with the manufacturer's instructions.  RNA-Seq on SKOV3 cells was performed by LC-Bio Technology (Hangzhou, China), involving library preparation with poly-A enrichment, cDNA synthesis, and sequencing on an Novaseq™ 6000 platform.
ENA first public
2025-12-31
INSDC center name
Research Institute for Reproductive Medicine and Genetic Diseases, Wuxi Maternity and Child Health Care Hospital
INSDC status
public
Submitter Id
E-MTAB-14740:siCDIPTOSP_2
broker name
ArrayExpress
cell_line
SKOV3
cell_type
epithelial cell
collection date
not collected
common name
human
developmental stage
adult
disease
ovarian cancer
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
ovary
sample name
E-MTAB-14740:siCDIPTOSP_2
scientific_name
Homo sapiens