N1_Cre_Iso
Mus musculus
Protocols: Primary BMDMs from CX3CR1-Cre LRRC8A flox and wild-type littermates (CX3CR1-cre negative, LRRC8A flox) were incubated in DMEM + 50% PBS or 50% H2O for 4 hours.   BMDMs were lysed in RNA lysis buffer (Purelink RNA miniprep kit). RNA was isolated using a Purelink RNA miniprep kit following the manufacturer's instructions.  RNA samples were assessed for quality and integrity using a 2200 TapeStation (Agilent Technologies) and then libraries were generated using the TruSeq® Stranded mRNA assay (Illumina, Inc.) according to the manufacturer's protocol.
ENA first public
2026-03-15
INSDC center name
Division of Neuroscience, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK. Geoffrey Jefferson Brain Research Centre, The Manchester Academic Health Science Centre, Northern Care Alliance NHS Foundation Trust, University of Manchester, Manchester, UK. Lydia Becker Institute of Immunology and Inflammation, University of Manchester, Manchester, UK.
INSDC status
public
Submitter Id
E-MTAB-16723:N1_Cre_Iso
broker name
ArrayExpress
cell type
Primary bone marrow-derived macrophage
collection date
not collected
common name
house mouse
genotype
CX3CR1-Cre positive; Wild-type (CX3CR1 cre control)
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
bone marrow
replicate
n1
sample name
E-MTAB-16723:N1_Cre_Iso
scientific_name
Mus musculus
stimulus
Isotonic (4 h)
strain
C57BL/6J