MDAt41_sgRAIN_2
Homo sapiens
Protocols: Cells were collected after 10 days from selection. TPC1 cells were cultured in DMEM medium supplemented with 10% FBS and 1% P/S. MDA-T41 cells were grown in RPMI-1640 supplemented with 1% non-essential amino acids, 10% FBS and 1% penicillin-streptomycin. All cell lines were grown at 37°C/ 5% CO2 and routinely tested for mycoplasma infection. Cells were infected with pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro containing either sgRAIN, sgRUNX2 or sgNT, then selected with 1 µg/ml Puromycin for 3 days. Total RNA was extracted by Maxwell RSC simplyRNA Cells kit (Promega, Madison, Wisconsin, USA) Libraries were obtained starting from 100ng of total RNA following the Illumina Stranded Total RNA preparation Ligation with Ribo-Zero Plus protocol.
ENA first public
2025-12-31
INSDC center name
Laboratory of Traslational Research. Azienda USL-IRCCS di Reggio Emilia
INSDC status
public
Submitter Id
E-MTAB-13777:MDAt41_sgRAIN_2
broker name
ArrayExpress
cell_line
MDA-T41
collection date
not collected
common name
human
developmental stage
adult
disease
papillary thyroid carcinoma
genetic modification
RAIN_sgRNA
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
thyroid gland
replicate
2
sample name
E-MTAB-13777:MDAt41_sgRAIN_2
scientific_name
Homo sapiens