Platform Comparison Barnyard K562:3T3
mixed sample
K562 (ATCC) and 3T3 cells (ATCC) were harvested and washed twice in PBS containing 0.4% BSA, palleted at 300 x g for 5 minutes in a swing bucket centrifuge (4C). Cell pellets were resuspended in 400 l of room-temperature Fluent Cell Resuspension Buffer (FB0002440) and analyzed for cell count and viability using a Nexcelom Cellometer Auto 2000 (Nexcelom Bioscience) with Acridine Orange (AO) / Propidium Iodide (PI) staining (viability: 99.7% for K562; 98.8% for 3T3). The cells were then combined at a 1:1 ratio to a final concentration of 2,000 cells/l for PIP-seq capture. The mixed-species cell concentration was reverified using the Cellometer Auto 2000 (Nexcelom Bioscience) prior to single-cell capture.
component organism
Homo sapiens
component organism
Mus musculus
biomaterial_provider
ATCC:CCL-243; ATCC:CRL-1658
collection_date
N/A
geo_loc_name
N/A
sex
not applicable
tissue
Bone, Marrow; Embryo
cell_line
K562; 3T3
cell_type
Lymphoblast:Fibroblast
disease
Chronic Myelogenous Leukemia Cml; None
sample_type
cell culture mix