M170_40Gy_Rep1
Homo sapiens
Protocols: Cell monolayers were trypsinized, neutralized with medium, washed with PBS, and centrifuged. Washed cell pellets were resuspended in Macherey-Nagel Nucleospin RNA Plus Lysis Buffer. No dissections were performed so this protocol is not applicable. MEM + 10%FBS + sodium pyruvate + non-essential amino acids + Gluta-Max + pen/strep in 5% 02 + 5% CO2 20 or 40 Gy X-rays at 120 kV generated by a Faxitron 43855-D generator The following software were used for data conversion: EdgeR Limma voomrmalization and data  RNA was extracted with the Macherey-Nagel Nucleospin RNA Plus. NEBNext Ultra II Directional RNA Library Kit with PolyA RNA selection
ENA first public
2026-06-30
INSDC center name
I2BC/UMR9198-CEA/CNRS/Université Paris-Saclay
INSDC status
public
Submitter Id
E-MTAB-14201_2:M170_40Gy_Rep1
broker name
ArrayExpress
cell line
primary cell line
cell type
fibroblast
collection date
not collected
common name
human
developmental stage
adult
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
irradiate
40 Gy X-ray 120kV
isolate
not applicable
organism part
fibroblast
sample name
E-MTAB-14201_2:M170_40Gy_Rep1
scientific_name
Homo sapiens