mef_03
Mus musculus
Protocols: For two-dimensional co-culture, SCC25-tdTomato cells were seeded in a 1:1 ratio with mouse or naked mole-rat fibroblasts in DMEM/F12 medium (11320-032, Life Technologies) supplemented with 0.05% FBS (Life Technologies), 400 ng/ml hydrocortisone (Santa Cruz Biotechnology) and 1% Penicillin-Streptomycin (PEST) and cultured in a 37°C incubator with 5% CO2. After 48 hours SCC25-tdTomato and fibroblasts were separated using FACS sorting (E-cadherin-APC staining to separate SCC25 from fibroblasts (TdTom &E-cadherin-). Approximately 50,000-100,000 cells were sorted of each cell type from each condition. Total RNA was extracted by adding 800 µl TRIzol reagent (Life Technologies). Total RNA samples were DNased treated using Turbo DNase (Life Technologies). RNA-sequencing libraries prepared using Illumina stranded total RNA Prep, Ligation with RiboZero Plus (Illimuna) following manufacturer's instructions.
ENA first public
2025-12-31
External Id
SAMEA114624326
INSDC center name
German Cancer Research Center (DKFZ)
INSDC last update
2023-11-15T20:28:45Z
INSDC status
public
Submitter Id
E-MTAB-13538:mef_03
broker name
ArrayExpress
cell_line
MEF3T3
cell_type
fibroblast
collection date
not collected
common name
house mouse
developmental stage
embryo
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
growth condition
Mouse fibroblasts alone
isolate
not applicable
organism part
embryo
sample name
E-MTAB-13538:mef_03
scientific_name
Mus musculus