Integrating megabarcoding and metabarcoding to unlock diversity and distribution data shortfalls in dark taxa
soil metagenome
Amplicon sequencing of 6,016 individually processed soil arthropod specimens, including 5,609 mites (Acari) and 407 springtails (Collembola), along with 128 negative controls. Each specimen was PCR-amplified using indexed primers targeting a ~650 bp fragment of the mitochondrial COI barcode region. Amplicons were subsequently pooled into a single library and sequenced on an Oxford Nanopore GridION platform (Oxford Nanopore Technologies). Basecalling was performed using Dorado, and reads are provided as a single FASTQ file prior to demultiplexing. Information required for demultiplexing and for linking sequences to individual specimens, including primer indices and specimen-level metadata, is available in Dryad (https://doi.org/10.5061/dryad.j9kd51ct5).
isolate
not applicable
isolation_source
soil samples
tissue
full body
collection_date
2016/2023
geo_loc_name
Spain: Canary Islands
collected_by
Island Ecology and Evolution Group (GEEI)
identified_by
M.L. Moraza and E. Mateos
biomaterial_provider
Institute of Natural Products and Agrobiology (IPNA-CSIC)
breed
not applicable
lat_lon
missing
BioSampleModel
Metagenome or environmental