G49
Homo sapiens
Protocols: Skin explants were transferred immediately into culture medium. Prior to experimental manipulation, explants were equilibrated for at least 1 h at 37 °C in a humidified atmosphere containing 5% CO₂. Explants were cultured in 12-well plates for up to 5 days in a proprietary, chemically defined, hydrocortisone- and serum-free medium supplemented with penicillin (100 µg/mL) and streptomycin (100 µg/mL). Cultures were maintained at 37 °C and 5% CO₂, and culture medium was replaced daily. Skin explants were treated topically once daily with either vehicle control (Laboratoire Gola, Galenicare SA) or VTAMA (tapinarof cream, 1%, Dermavant Sciences) throughout the culture period. Total RNA from Genoskin samples was isolated using the RNeasy Lipid Tissue Kit (Cat. 74804, Qiagen) according to the manufacturer's instructions.  Illumina TruSeq Stranded mRNA protocol (Directional, first strand)
ENA first public
2026-07-26
INSDC center name
Department of Dermatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland
INSDC status
public
Submitter Id
E-MTAB-17321:G49
age
25-65
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
skin of abdomen
sample name
E-MTAB-17321:G49
scientific_name
Homo sapiens