H3K27ac_rep1
Homo sapiens
Protocols: 20M cells were collected for Chromatin Immunoprecipitation. MDA-T41 cells were grown in RPMI-1640 supplemented with 1% non-essential amino acids, 10% FBS and 1% penicillin-streptomycin. All cell lines were grown at 37°C/ 5% CO2 and routinely tested for mycoplasma infection. ChIP samples were purified with PCR Purification Kit (Qiagen) and eluted in 50ul of Elution Buffer. Libraries were obtained starting from 3ng of ChIP and Input samples following ThruPLEX DNA-Seq Kit (Takara Bio Ink) protocol.
ENA first public
2025-12-31
INSDC center name
Laboratory of Traslational Research, Azienda USL-IRCCS di Reggio Emilia
INSDC status
public
Submitter Id
E-MTAB-14152:H3K27ac_rep1
broker name
ArrayExpress
cell_line
MDA-T41
cell_type
Epithelial-like
collection date
not collected
common name
human
developmental stage
adult
disease
papillary thyroid carcinoma
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
thyroid gland
replicate
1
sample name
E-MTAB-14152:H3K27ac_rep1
scientific_name
Homo sapiens