coffee farm soil sample
uncultured soil microorganism
bulk top-soil sample from a smallholder coffee farm (coffee, banana, other trees, and annual crops)
elevation
1212
amount or size of sample collected
2
sample volume or weight for DNA extraction
1
organism
uncultured soil microorganism
broad-scale environmental context
tropical moist broadleaf forest biome (ENVO_01000228)
collection date
2023-07
soil texture measurement
26/40/34
target gene
18S SSU rRNA
geographic location (longitude)
33.04455748
environmental medium
tropical soil (ENVO_00005778)
geographic location (latitude)
0.42050989
scientific_name
uncultured soil microorganism
target subfragment
AMV4.5N_AMDGR
geographic location (region and locality)
Lake Victoria Crescent
pcr primers
AAGCTCGTAGTTGAATTTCG_CCCAACTATCCCTATTAATCAT
local environmental context
coffee plantation (ENVO_00000163)
depth
0.05-0.30
project name
PRJEB107292
pH
6.48
sample pooling
At each site, five samples were collected along a transect at an interval of 20 m (from 5-30 cm depth) and mixed to form a composite sample representative of the site
geographic location (country and/or sea)
Uganda