AA_3_L
Arabidopsis arenosa
DNA isolated from A. arenosa mature leaves.
ENA first public
2025-12-31
INSDC center name
CUNI
INSDC status
public
Submitter Id
AA208_2732-4_L
air temperature regimen
21/18 °C day/night
collection date
2023
geographic location (country and/or sea)
Slovakia
geographic location (latitude)
49.043514
geographic location (longitude)
20.180772
growth facility
growth chamber
isolation and growth condition
Seeds were surface sterilized with 5% sodium hypochlorite and 0.01% (v/v) of Triton X-100 and planted on Petri dishes with Murashige and Skoog medium (1/2× MS: M0222 Duchefa, 0.8% agar, 10 mM MES, pH 5.8). Planted seeds were stratified for 7 days at 4 °C in the dark and then germinated for 2 weeks at 21/18 °C and 16/8 hours day/night cycle. Seedlings were transferred to soil and cultivated in the same conditions for another 5 weeks. After this, the plants were vernalized for at least two months at 4 °C and 8/16 hours day/night cycle. Vernalized plants were grown under long-day conditions (21/18 °C and 16/8 hours day/night cycle) to induce flowering. Young leaves were collected into 2ml Eppendorf tubes. All samples were kept at -80 °C for further use. DNA was extracted from leaves using DNeasy® Plant Mini Kit (Qiagen).
light regimen
16/8 hours day/night
plant developmental stage
PO:0025034
plant growth medium
soil
plant structure
BTO:0000713
ploidy
diploid
propagation
manual crossing
sample collection method
DNA was extracted from leaves using DNeasy® Plant Mini Kit (Qiagen).
sample health state
healthy
sample name
AA208_2732-4_L
sampled age
adult plant
scientific_name
Arabidopsis arenosa
source material identifiers
AA208_2732
subspecific genetic lineage name
West Carpathian
subspecific genetic lineage rank
phylogenetic lineage