TvBb2
Trichomonas vaginalis
Protocols: T. vaginalis co-cultured with B. breve or T. vaginalis alone (negative control) were transferred into centrifuge tubes and centrifuged at 2500 rpm for 5 minutes. The supernatant was removed, and TRIzol reagent was added and mixed thoroughly. Co-culture experiments were conducted to investigate the direct interactions between T. vaginalis and B. breve. Mid-log phase bacteria were standardized to 10⁸ CFU, and parasites were prepared at 10⁷ cells. Both organisms were combined in serum-free YI-S and incubated anaerobically for four hours.  Total RNA was extracted using the Quick-RNA MiniPrep Kit (Zymo Research, Cat. No. R2052) according to the manufacturer's instructions. Briefly, cells were lysed in RNA Lysis Buffer, and the lysates were passed through spin columns for RNA binding. After on-column DNase I digestion to remove genomic DNA, the columns were washed and RNA was eluted in RNase-free water. RNA concentration and purity were determined using a NanoDrop spectrophotometer, and RNA integrity was verified with an Agilent Bioanalyzer. RNA-seq libraries were constructed using the Illumina Poly(A) Capture (Cat. No. 20777928), cDNA Synthesis (Cat. No. 20761354), and RNA Prep, Ligation (Cat. No. 20778789) kits according to the manufacturer's protocols.
ENA first public
2025-11-18
INSDC center name
s58141092@gs.ncku.edu.tw
INSDC status
public
Submitter Id
E-MTAB-16002:TvBb2
broker name
ArrayExpress
collection date
not collected
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-16002:TvBb2
scientific_name
Trichomonas vaginalis
stimulus
co-culture with Bifidobacterium breve
strain
ATCC 30236, ATCC 15700