WT_AP_5-2
Solanum tuberosum
Protocols: Samples gathered in two biological replicates.  Samples were taken from 5- and 7-week-old apical shoot parts and 3-week-old stolons  collected at ZT6 from four plants per pot.  Solanum tuberosum L., cv. Desirée wild-type (WT) plants and transgenic lines (amiRBBX20.2.7 and StBBX20-OE3) were propagated in vitro on solid MS medium at 20/18°C (day/night) under a 150 µmol photon m -2 s -1 PFD and a 14-hour photoperiod for three weeks. Then, four single-eye plugs from tubers were transferred to the soil (pots diameter 21 cm) and grown in the phytotron under conditions as follows: 20/18°C (day/night), 40% relative humidity, PFD of 350 µmol photons m -2 s -1 and 14-hour- light/10-hour-dark regime. The term Zeitgeber time (ZT) relates to the experimental time, where the ZT0 point corresponded to light-on (initiation of experimental dawn) and the ZT14 point to light-off (initiation of experimental dusk). Two independent experiments were conducted and two independent plant samples from each experiment were collected. 5- and 7-week-old apical shoot parts and 4-week-old stolons of S. tuberosum WT plants, and two transgenic lines: StBBX20 gene-silenced line (amiRBBX20.2.7) and StBBX20 gene-overexpressed line (StBBX20-OE3) were collected at ZT6. Total cellular RNA was prepared using TRI Reagent® RT (Molecular Research Center, Inc.) and treated with DNase I during RNA purification. The quality and quantity of RNA were verified using a DS-11 FX spectrophotometer (DeNovix Inc.). RNA integrity number (RIN) of samples sufficient for sequencing (≥ 7) and rRNA ratio (≥ 1.7) was checked using Bioanalyzer RNA Nano 6000 chip (Agilent Technologies, Inc.).  Library construction using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina)
ENA first public
2025-11-18
INSDC center name
Institute of Plant Genetics, Polish Academy of Sciences
INSDC status
public
Submitter Id
E-MTAB-15322:WT_AP_5-2
broker name
ArrayExpress
collection date
not collected
common name
potato
cultivar
Desiree
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
vegetative shoot apical meristem
sample name
E-MTAB-15322:WT_AP_5-2
scientific_name
Solanum tuberosum