chondrogenesis_GLP1R_KD_3
Homo sapiens
Protocols: PDLSCs were separated from the surface of the middle third of the root, and were digested with α-minimum essential medium (α-MEM, Gibco, Carlsbad, CA, USA) containing 3 mg/mL collagenase (type I) and 4 mg/mL dispase (both from Sigma-Aldrich, Billerica, MA, USA) for 15 min at 37 °C. Single-cell suspensions were obtained by passing the digested tissues through a 70-μm cell strainer (BD Bioscience, Franklin Lakes, NJ, USA). Then the cell suspensions were seeded into 10-cm culture dishes (Corning, USA) containing α-MEM supplemented with 15% fetal bovine serum (FBS, Hyclone, New York City, NY, USA), and 1 x GlutaMAX (Thermo Fisher Scientific, Waltham, MA, USA). The cells were incubated at 37 °C in a humidified atmosphere containing 5% CO2, and medium was changed twice a week. Cells were used at passages 3-5 for subsequent experiments. SP600125 (MedChemExpress LLC, Shanghai, China) were dissolved in dimethyl sulfoxide (DMSO). Unless otherwise indicated, cells were pre-incubated with serum-free medium containing same dose of DMSO (0.2%) or 10 μM SP600125 for 2 h Total RNA of 5 x 10^7 PDLSCs were extracted using TRIZOL (Thermo Fisher Scientific). Nanodrop 2000 (Thermo Fisher Scientific) and Agilent Bioanalyzer 2100 (Agilent) were used to evaluate RNA concentration and quality. 5 μg of RNA from different experimental groups was prepared cDNA library using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer's instructions.
ENA first public
2025-07-25
INSDC center name
Suzhou Ninth People's Hospital
INSDC status
public
Submitter Id
E-MTAB-15372:chondrogenesis_GLP1R_KD_3
broker name
ArrayExpress
cell line
primary cell line
cell type
PDLSC
collection date
not collected
common name
human
developmental stage
adult
disease
Malocclusion
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
periodontal ligament
sample name
E-MTAB-15372:chondrogenesis_GLP1R_KD_3
scientific_name
Homo sapiens