Low-Ester 3
Fragaria x ananassa
Protocols: Fruits from contrasting lines with high or low MCE content. Each bulked pool was generated with equivalent amount of ripe fruit from 10 F1 lines from the '232' x '1392' mapping population selected based on high or low concentration of six selected medium-chain esters Standard commercial strawberry cultivation in the Huelva region (Spain) involves growing plants under plastic tunnel structures to optimize microclimate conditions. The soil is prepared through disinfection and then covered with black polyethylene mulch, which reduces weed growth, conserves soil moisture, and regulates soil temperature for improved plant establishment and yield. Total RNA was extracted from 300 mg fruit tissue following the CTAB protocol described in Gambino et al., 2008. The integrity of the RNA samples was assessed by agarose gel electrophoresis and further verified using a 2100 Bioanalyzer (Agilent, Folsom, CA), which resulted in RIN values ranging between 8.0 and 8.9 for the six samples. RNA was break in suitably small pieces by enzymatic treatment. After fragmentation, partial Illumina adapters were ligated to the RNA fragments' ends, which facilitates subsequent reverse transcription, amplification, and sequencing. Fragmentation and adapter ligation together ensure that RNA fragments are compatible with Illumina sequencing and retain strand information for antisense transcript detection.
ENA first public
2025-12-31
INSDC center name
Centro IFAPA Málaga
INSDC status
public
Submitter Id
E-MTAB-15704:Low-Ester 3
age
1
broker name
ArrayExpress
collection date
not collected
common name
strawberry
cultivar
F1 (232 x 1392)
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
pool of full ripe fruits
isolate
not applicable
organism part
fruit
phenotype
low esters
replicate
3
sample name
E-MTAB-15704:Low-Ester 3
scientific_name
Fragaria x ananassa