AireC313X_2_BM_GEX_Lane2
Mus musculus
Protocols: Thymi and lymph nodes were squashed and filtered through 70 μm MACS SmartStrainers (Miltenyi Biotech, cat. 130-110-916) and washed with 0.5% fetal bovine serum (FBS; Gibco, cat. 10082147) in phosphate-buffered saline (PBS).  Bone marrow immune cells were isolated by washing with with 0.5% fetal bovine serum (FBS; Gibco, cat. 10082147) in phosphate-buffered saline (PBS) Dead dells from all three tissues were subsequently removed with Dead cell removal kit (Miltenyi Biotech, cat. 130-090-101).  Ten thousand cells (counted in duplicate) from each thymus, lymph node and bore marrow sample were prepared with Chromium Next GEM Single Cell 5' Kit v2 (10x Genomics, cat. PN-1000286) and run on the Chromium Controller (10x Genomics). Gene expression libraries were prepared using the same single cell kits, V(D)J libraries were prepared with Chromium Single Cell Mouse TCR Amplification Kit (10x Genomics, cat. PN-1000254) and Chromium Single Cell Mouse BCR Amplification Kit (10x Genomics, cat. PN-1000255) and additional Library Construction Kits (10x Genomics, cat. PN-000352).
ENA first public
2025-03-01
INSDC center name
Department of Clinical Science, University of Bergen, Norway
INSDC status
public
Submitter Id
E-MTAB-14774:AireC313X_2_BM_GEX_Lane2
age
69
broker name
ArrayExpress
cell_type
immune cell
collection date
not collected
common name
house mouse
developmental stage
postnatal
genotype
AireC313X
geographic location (country and/or sea)
not collected
individual
AireC313X_2
isolate
not applicable
organism part
bone Marrow
sample name
E-MTAB-14774:AireC313X_2_BM_GEX_Lane2
scientific_name
Mus musculus
sex
male
strain
C57BL/6