8373605
Homo sapiens
Protocols: Patients with papillary thyroid carcinoma (PTC) underwent thyroidectomy at Yonsei Cancer Center or Yongin Severance Hospital. Resected tissues were snap-frozen in liquid nitrogen immediately and stored at –80 °C. Cytology was classified as Bethesda V/VI, and risk was stratified using ATA 2015 guidelines. Total RNA was extracted using TRIzol® reagent (Invitrogen) per manufacturer's instructions. RNA concentration was measured using Quant-IT RiboGreen assay and quality evaluated using Agilent TapeStation. Only RNA with RIN ≥ 7.0 was used. RNA libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit. mRNA was isolated with poly-T magnetic beads, fragmented, and converted to cDNA, followed by end repair, adapter ligation, and PCR enrichment.
ENA first public
2025-07-25
INSDC center name
Department of Surgery, Yonsei University College of Medicine
INSDC status
public
Submitter Id
E-MTAB-15233:8373605
age
47
broker name
ArrayExpress
clinical history
Cluster 2 Low
collection date
not collected
common name
human
developmental stage
adult
disease
thyroid cancer
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
thyroid gland
sample name
E-MTAB-15233:8373605
scientific_name
Homo sapiens
sex
female