C5
Mus musculus
Protocols: After anesthesia with tribromoethanol, the ventricle was obtained after systemic perfusion with PBS. Tissues of ~20mg were harvested and stored at -80°C. Using the regular TRIzol method. Library was prepared using Optimal Dual-mode mRNA Library Prep Kit (BGI-Shenzhen). Library preparation is performed using Optimal Dual-mode mRNA Library Prep Kit (BGI-Shenzhen, China). A certain amount of RNA are denatured at suitable temperature to open the secondary structure, and mRNA is enriched by oligo (dT) attached magnetic beads. After reacting at a suitable temperature for a fixed time period, RNAs are fragmented with fragmentation reagents.  Then First-strand cDNA is generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. The synthesized double strand cDNA is subject to end repairment reaction. After cDNA end repairment, a single 'A' nucleotide is added to the 3' ends of the blunt fragments through A tailing reaction. Then the reaction system for adaptor ligation configured to ligate adaptors with the cDNAs, and finally, the library products are amplified through PCR reaction and subjected to quality control.  Next, the single-stranded library products are produced via denaturation. The reaction system for circularization is set up to get the single-stranded cyclized DNA products. Any uncyclized single stranded linear DNA molecules will be digested.
ENA first public
2025-12-31
INSDC center name
Fuwai Hospital, CAMS&PUMC
INSDC status
public
Submitter Id
E-MTAB-15255:C5
age
17
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
disease
heart failure
disease staging
Healthy control
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
C5
isolate
not applicable
organism part
heart
sample name
E-MTAB-15255:C5
scientific_name
Mus musculus
sex
male
strain
C57BL/6N