Genomic DNA from Saccharina latissima strain ALO20
Saccharina latissima
DNA extracted from culture sporophyte blade tissue using the Macherey Nagel Nucleospin plant II kit.
ENA first public
2026-06-30
INSDC center name
SBR
INSDC status
public
Submitter Id
ALO20
additional_details
ALO20 was an F1 hybrid sporophyte derived from a cross between clonal female gametophyte strain GA_STG_57_F10 (derived from a wild sporophyte collected at Saint-Gu�nol�, Brittany, France by Mathieu Oriot on 05/09/2015) and male gametophyte strain GA_PEN_28 (derived from a wild sporophyte collected at Penmarc�h, Brittany, France by Philippe Potin on 21/02/2023)
collection date
2025-05-06
extraction_date
2025-12-02
extraction_method
DNA was extracted using the Macherey-Nagel Nucleospin Plant II Kit (Macherey-Nagel GmbH & Co. KG, Germany) with minor protocol adaptation. Sporophyte tissue was ground in liquid nitrogen using a mortar and pestle. Between 120 and 200 mg of fine powder was then transferred to 3200 �L of lysis buffer PL1 supplemented with 5% PVP40 (w/v) and prewarmed to 60�C. Lysis was carried out for 10 min at 60�C with 1000 rpm agitation and then at room temperature for 2.5 h. A centrifugation step at 11,000g for 2 min was added before the filtration in order to remove debris and prevent column clogging. An additional wash with PW1 and with PW2 buffer was carried out to further eliminate PCR inhibitors. DNA was eluted into 60 �L of PE buffer at 55�C. DNA concentration and quality was measured using both the NanoDrop One and the Qubit 4 (dsDNA HS kit) and the quality of the extracted DNA was assessed by PCR amplification of an ITS marker using specific primers. A260/A280 1.787, A260/A230 1.375.
generation
sporophyte
geographic location (country and/or sea)
France
growth condition
FI hybrid sporophytes were generated by combining male and female pairs of clonal gametophyte strains in 55 mm Petri dishes with Provasoli-enriched seawater and cultivating at 10�C under white light. As they grew, the sporophyte progeny were then transferred to 140 mm Petri dishes, then to open-flow Low Intervention Culture System (LICSy) systems and then to 300 L and 4000 L tanks, where they were grown suspended from cotton strings. Finally, coppiced sporophytes from the 4000 L tank cultures were co-cultivated for two months in a single 3000 L tank in natural seawater with bubbling and a hydrodynamic current before harvesting tissue for DNA extraction. F1 hybrid sporophyte blade tissue was frozen in liquid nitrogen and stored at -80�C.
harvest_date
2025-05-06
ploidy
diploid
sample name
ALO20
sample storage conditions
Frozen
sample storage temperature
-80
scientific_name
Saccharina latissima
strain
ALO20
tissue_type
blade