WT_2
Homo sapiens
Protocols: Single nucleotide replacement in the human TIA1 gene (E384K) in human EndoC-betaH1 cells was achieved by prime editing. Total RNA was isolated from five wildtype (WT 1-5) and three mutated (Mut 1-3) EndoC-betaH1 human beta-cell line. Total RNA was extracted by QIAGEN RNeasy Mini Kit, following manufacturer's instructions. Total RNA was subjected to rRNA depletion, fragmented, and converted into double-stranded cDNA. After end-repair, A-tailing, and adapter ligation, libraries were PCR-amplified and sequenced on an Illumina platform using paired-end reads.
ENA first public
2026-06-30
INSDC center name
UU
INSDC status
public
Submitter Id
E-MTAB-16316:WT_2
broker name
ArrayExpress
cell line
EndoC-betaH1
cell type
human beta-cell line EndoC-betaH1
collection date
not collected
common name
human
developmental stage
adult
disease
metabolic disease
genotype
human TIA1 gene (E384K wild type)
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
pancreas
sample name
E-MTAB-16316:WT_2
scientific_name
Homo sapiens