Control
Mus musculus
Protocols: After mouse sacrifice hearts were excised and retrograde perfused with first 5ml Heparin/PBS solution followed by 1ml PBS. Perfused hearts were place in OCT, snap-frozen in pre-cooled isopentane (-30 to -40°C) and stored at -80°C until further use. 10µm thick tissue sections were cut in a cryostat at -20°C chamber temperature and -10°C object head temperature and placed at the 10X Visium Gene Expression slide. Prepared Visium slides were used as input for the Visium Spatial Gene Expression workflow v1 (10X Genomics, Pleasanton, USA) according to the manufacturer's instructions. Tissue permeabilization was carried out for 12 minutes Library construction was performed to manufacturer's instructions, the Visium Spatial Gene Expression workflow (10X Genomics, Pleasanton, USA).
ENA first public
2025-03-02
External Id
SAMEA112716317
INSDC center name
Institut fur Pharmakologie Medizinische Fakultat der Heinrich-Heine-Universitat Dusseldorf
INSDC last update
2023-03-02T11:31:34Z
INSDC status
public
Submitter Id
E-MTAB-12700:Control
age
14
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
iAdipoQCre: wt, ATGL fl/fl
individual
283076
injury
ischemia reperfusion injury
isolate
not applicable
organism part
heart
sample name
E-MTAB-12700:Control
scientific_name
Mus musculus
sex
male
strain
B6.129-Pnpla2tm1Eek/J Tg(Adipoq-cre/ERT2)1Soff/J