miRNA_NC-LLCExos
Mus musculus
Protocols: Exosomes were collected and purified from the cell culture supernatant of NC-LLC and BM-LLC cells via differential ultracentrifugation. Lewis lung carcinoma (LLC) cells (NC-LLC and BM-LLC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% exosome-depleted fetal bovine serum (FBS) at 37°C in a 5% CO₂ atmosphere. Total RNA, including small RNAs, was extracted from the purified exosomes using the miRNeasy Serum/Plasma Kit (Qiagen) according to the manufacturer's instructions. Small RNA libraries were constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina. Briefly, small RNA molecules were ligated to 3' and 5' adapters, reverse transcribed, and amplified by PCR. The final cDNA libraries were size-selected to enrich for fragments containing microRNAs.
ENA first public
2025-12-31
INSDC center name
nbdeyy
INSDC status
public
Submitter Id
E-MTAB-15763:miRNA_NC-LLCExos
broker name
ArrayExpress
cell line
LLC
cell type
lung cancer cell
collection date
not collected
common name
house mouse
disease
normal
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-15763:miRNA_NC-LLCExos
scientific_name
Mus musculus