WT FGSCs-m6A-IP-Rep 2
Mus musculus
Protocols: FGSCs were cultured on STO feeder cells using Minimum Essential Medium-ɑ, supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 1 mM non-essential amino acid , 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 10 ng/mL mouse epidermal growth factor , 10 ng/mL mouse leukemia inhibitory factor, 40 ng/mL mouse glial cell line-derived neurotrophic factor, 10 ng/mL human basic fibroblast growth factor, and 1% penicillin-streptomycin. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected. Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer's protocols. RNA was purified by NEBNext® rRNA Depletion Kit (NEB, USA). Fragmented and immunoprecipitated with anti-m6A antibody (Synaptic Systems, 202003). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).
ENA first public
2025-07-26
INSDC center name
SJTU
INSDC status
public
Submitter Id
E-MTAB-14256:WT FGSCs-m6A-IP-Rep 2
broker name
ArrayExpress
cell_line
FGSC
cell_type
germ line stem cell
collection date
not collected
common name
house mouse
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
ovary
sample name
E-MTAB-14256:WT FGSCs-m6A-IP-Rep 2
scientific_name
Mus musculus