WZ12_CADASIL_Control
Homo sapiens
Protocols: CADASIL patient and control iPSCs were differentiated into neural crest-specific VSMCs. Neural crest progenitors were generated by culturing iPSCs on vitronectin-coated plates under defined Essential 6 medium (Gibco; A1516401) with 10 ng/mL FGF2 (PeproTech; 100-18B) and 10 μM SB431542 (Tocris; 1614) that promote neural crest specification. Cells were first passaged at day 5 and subsequently upon reaching confluence, with continued supplementation of FGF2 and SB431542, up to passage 12. After this intermediate stage, cells were dissociated and transferred into SMC differentiation medium composed of Essential 6 supplemented with 10 ng/mL PDGF-BB (PeproTech; 100-14B) and 2 ng/mL TGF-β1 (PeproTech; 100-21) for a minimum of 12 days. Neural crest-derived VSMCs reached maturity by day18, at which point total RNA was isolated from the fully differentiated iPSC-derived VSMCs for RNA-seq analysis. The induced pluripotent stem cells were cultured in Essential 8 Medium (Thermo Fisher Scientific) Total RNA from CADASIL patient and control iPSCs derived neural crest-specific VSMCs was extracted using the RNeasy Mini Kit (Qiagen). Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol.
ENA first public
2025-12-12
INSDC center name
UOM-BCF
INSDC status
public
Submitter Id
E-MTAB-16303:WZ12_CADASIL_Control
broker name
ArrayExpress
cell type
iPSC differentiated vascular smooth muscle cell
collection date
not collected
common name
human
developmental stage
adult
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
skin of body
sample name
E-MTAB-16303:WZ12_CADASIL_Control
scientific_name
Homo sapiens