BM-LLC
Mus musculus
Protocols: Total RNA was extracted from RAW264.7 cells after 72 hours of exosome treatment using Trizol reagent (Invitrogen, #15596026). RAW264.7 murine macrophage cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Cells were maintained at 37°C in a humidified incubator with 5% CO₂. RAW264.7 cells were treated with 10 μg/mL of exosomes (isolated from NC-LLC or BM-LLC cell culture supernatants) for 72 hours. The culture medium was refreshed every 48 hours. Total RNA was extracted from cells using Trizol reagent (Invitrogen, #15596026) according to the manufacturer's instructions. RNA quality and concentration were assessed using a NanoDrop spectrophotometer. RNA libraries for sequencing were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). Briefly, mRNA was enriched, fragmented, and reverse-transcribed into cDNA. After end repair and adapter ligation, libraries were amplified by PCR. The final library quality was checked using an Agilent Bioanalyzer.
ENA first public
2025-12-31
INSDC center name
nbdeyy
INSDC status
public
Submitter Id
E-MTAB-15762:BM-LLC
broker name
ArrayExpress
cell line
RAW264.7
cell type
macrophage
collection date
not collected
common name
house mouse
disease
normal
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-15762:BM-LLC
scientific_name
Mus musculus
stimulus
BM-LLC exosomes treated