Hela cell lysate
Homo sapiens
Protocols: HeLa cells were grown under standard conditions and harvested for RNA extraction. RNA extraction was performed using the RNeasy Micro Kit (Qiagen) following the manufacturer's instructions. RNA was quantified using Qubit 4.0 Fluorometer and integrity checked using RNA Kit on Agilent 5600 Fragment Analyzer. rRNA was depleted using QIAseq FastSelect -rRNA HMR Kit and NEBNext rRNA Depletion Kit (Human/Mouse/Rat). Strand-specific libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Briefly, rRNA-depleted RNA was fragmented, first and second strand cDNA synthesised with dUTP incorporation, adenylated at 3' ends, ligated with indexed adapters, and amplified by limited cycle PCR. dUTP incorporation enabled second strand degradation to maintain strand specificity.
ENA first public
2026-06-13
INSDC center name
University of Cambridge
INSDC status
public
Submitter Id
E-MTAB-17121:Hela cell lysate
broker name
ArrayExpress
cell line
HeLa
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
cervix
sample name
E-MTAB-17121:Hela cell lysate
scientific_name
Homo sapiens