AM_ATAC_IL33_rep3
Mus musculus
Protocols: Alveolar macrophages were collected by bronchoalveolar lavage (BAL). Single-cell suspension was stained with monoclonal antibodies followed by cell sorting. Additional information can be found in original publication. ATAC-seq was carried out with minor adaptations from the protocol  described in the following reference:  \"Buenrostro, J. D., Wu, B., Chang, H. Y. & Greenleaf, W. J. ATAC-seq: A method for assaying chromatin accessibility genome-wide. Curr Protoc Mol Biol 2015, 21.29.1-21.29.9 (2015).\"  Additional information can be found in original publication. Transposed DNA was amplified using a NEBNext High Fidelity 2× PCR Master Mix (New England Biolabs) with indexed primers. Libraries were purified using SPRIselect beads (Beckman Coulter) to remove primer dimers.
ENA first public
2025-12-13
INSDC center name
VIB-UGent Inflammation Research Center
INSDC status
public
Submitter Id
E-MTAB-16048:AM_ATAC_IL33_rep3
broker name
ArrayExpress
cell type
alveolar macrophage
collection date
not collected
common name
house mouse
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lung
sample name
E-MTAB-16048:AM_ATAC_IL33_rep3
scientific_name
Mus musculus
sex
female
stimulus
IL-33 (3x)
strain
C57BL6J