Nalm-6 VitC treated 05-10-22
Homo sapiens
Protocols: CD19-CAR-T cells were generated from human primary CD3+ T cells of three donors in presence or absence of vitamin C. RNA was isolated after 24h of co-culture with Nalm-6 cells. Details of experimental design can be found in the M&M section of the manuscript.  RNA was isolated using the RNeasy Plus Kit (Qiagen)  The RNA sequencing library was generated from 500 ng total RNA using the Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher Scientific) for mRNA purification, followed by the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) according to the manufacturer's protocols.
ENA first public
2025-11-19
INSDC center name
MHH
INSDC status
public
Submitter Id
E-MTAB-15708:Nalm-6 VitC treated 05-10-22
broker name
ArrayExpress
cell type
Nalm-6
collection date
not collected
common name
human
developmental stage
adult
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-15708:Nalm-6 VitC treated 05-10-22
scientific_name
Homo sapiens