ApoEChimp_2
Homo sapiens
Protocols: 5 million cells were homogenized in TRIzol reagent using a BeadBug Benchtop Homogenizer. Brain tissue was suspended in 1 mL of TRIzol and homogenized for 6 rounds of 10 seconds homogenization and 60-second holds between each round. Following homogenization, 300 µL of chloroform was added, and samples were centrifuged with heavy gel phase-lock tubes (VWR, 10847-802) to separate the aqueous phase. RNA purification kit (Quantabio, Extracta Plus, Cat# 95214-050) was used following the manufacturer's protocol. RNA concentration and integrity were assessed with a Qubit 4.0 Fluorometer and Agilent Bioanalyzer prior to sequencing.  Library preparation (mRNA library, poly A enrichment) was performed by Novogene.
ENA first public
2026-06-30
INSDC center name
USC
INSDC status
public
Submitter Id
E-MTAB-16242:ApoEChimp_2
broker name
ArrayExpress
cell line
U-87 MG
cell type
glial brain cell
collection date
not collected
common name
human
developmental stage
adult
disease
astrocytoma (excluding glioblastoma)
genotype
ApoEChimp
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
brain
sample name
E-MTAB-16242:ApoEChimp_2
scientific_name
Homo sapiens
stimulus
DMSO Control