HFD-EXO
Mus musculus
isolate
The blood samples were collected from HFD mice, left at room temperature for 30 min, and centrifuged for 15 min (4500 g) to collect the supernatant. The serum was re-centrifuged for 60 min (10000 g) to remove cellular debris and large vesicles. The supernatant was ultracentrifuged at 150000 g for 2 h. The precipitate was resuspended in PBS and ultracentrifuged again (150000 g, 2 h) to obtain the exosomes. Exosomes were resuspended in PBS and collected after filtration through a cell strainer.
breed
C57BL/6
age
16 weeks
dev_stage
Adulthood
collection_date
2023-07-30
geo_loc_name
China: Changchun
sex
male
tissue
blood
BioSampleModel
Model organism or animal