Sample 7
Mus musculus
Protocols: Animal procedures were approved by the local Ethics Committee No. 121 and were conducted in compliance with the ARRIVE guidelines. Animals were sedated and received a terminal injection of pentobarbital (150 mg/kg, i.p.). Mouse brains were removed, and the frontal cortex (from bregma levels 3.20 to 0.38) was dissected, weighed, rapidly frozen, and stored at −80 °C. Brain tissue was homogenized in 1mL TRIzolTM and total RNA was isolated using RNeasy micro kit RNA extraction according to the manufacturer's instructions (Macherey-Nagel, France). RNA was eluted in RNase/DNase free water. The quality and concentration of the RNA extracts were assessed by spectrophotometry using the NanodropTM apparatus (Thermofisher Scientific, MA, USA) Messenger (polyA+) RNAs were purified from 1000 ng of total RNA using oligo(dT). Libraries were performed using the strand specific RNA-Seq library preparation Stranded mRNA Prep, Ligation kit (Illumina)
ENA first public
2026-06-13
INSDC center name
GenomiqueENS, Institut de Biologie de l'ENS (IBENS), Département de biologie, École normale supérieure, CNRS, INSERM, Université PSL
INSDC status
public
Submitter Id
E-MTAB-15335:Sample 7
age
60
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
mouse postnatal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
CF-3-WT
isolate
not applicable
maternal genotype
Tph1 +/+
organism part
frontal cortex
sample name
E-MTAB-15335:Sample 7
scientific_name
Mus musculus
sex
male
strain
C57BL/6