Sample 5
Mus musculus
Protocols: 4-NQO was administered to 8 weeks old WT and TNCKO mice, which had been backcrossed with C57BL/6J mice for over 10 generations. The compound was delivered through drinking water at a final concentration of 100 μg/ml for 16 weeks. Following this treatment, the mice received a single 2 Gy dose of photon irradiation under anesthesia . After irradiation, the animals were provided with regular water for 6 weeks prior to sacrifice. At the time of euthanasia, tongue and mandibular lymph nodes (Tumor draining Lymph Nodes (TdLNs) were harvested.  Total RNA was extracted from lymph node samples using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions. RNA quality and concentration were assessed using a NanoDrop spectrophotometer and Agilent Bioanalyzer 2100. Only samples with RNA Integrity Number (RIN) ≥ 8 were used for library preparation and downstream RNA-seq analysis. RNA-seq libraries were prepared using NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and NEBNext Ultra II Directional RNA Library Prep Kit Illumina kits.
ENA first public
2025-12-12
INSDC center name
INSERM U1109 HOPITAL CIVIL Institut d'Hématologie et d'Immunologie 1, Place de l'Hôpital 67091 Strasbourg Cedex
INSDC status
public
Submitter Id
E-MTAB-16360:Sample 5
age
12
broker name
ArrayExpress
collection date
not collected
common name
house mouse
disease
head and neck carcinoma
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lymph node
sample name
E-MTAB-16360:Sample 5
scientific_name
Mus musculus
strain
C57BL/6J