Exp2_WT_lung_1xIL33
Mus musculus
Protocols: Exp2: Mice were treated with PBS, 1 x 100 ng IL-33 intratreacheally or 3 x 100 ng IL-33 intratracheally. Mice were sacrificed 24h after the last treatment and single cell lung suspensions were created. Cells were incubated with FACS antibodies as well as TotalSeq A hashtag antibodies. 12000 CD45-, 3000 CD45+ CD11c+ SiglecF+ alveolar macrophages, 3000 CD45+ lineage- CD11c- SiglecF- CD90+ ST2+ ILC2s and 12000 AM and ILC2 excluded CD45+ cells were FACS purified per mouse and pooled together per condition. Cells were diluted to a final concentration of 1000/μl and loaded on the 10X Chromium device. The DNA libraries were prepared using the GemCode Single Cell 3′ Gel Bead and Library kit, version NextGEM 3.1 (10× Genomics) according to the manufacturer's instructions with the addition of amplification primers (3 nM, 5′CCTTGGCACCCGAGAATT*C*C and 5′GTGACTGGAGTTCAGACGTGTGC*T*C) during cDNA amplification to enrich the TotalSeq-A cell surface protein and/or hashtag oligos. Size selection with SPRIselect Reagent Kit (Beckman Coulter, B23318) was used to separate amplified cDNA molecules for 3′ gene expression and cell surface protein construction. TotalSeq-A protein library construction including sample index Polymerase Chain Reaction (PCR) using Illumina's Truseq Small RNA primer sets and SPRIselect size selection was performed according to the manufacturer's instructions.
ENA first public
2025-12-13
INSDC center name
VIB-UGent Inflammation Research Center
INSDC status
public
Submitter Id
E-MTAB-16099:Exp2_WT_lung_1xIL33
broker name
ArrayExpress
cell type
immune and non-immune
collection date
not collected
common name
house mouse
disease
1 x IL33 treated
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lung
sample name
E-MTAB-16099:Exp2_WT_lung_1xIL33
scientific_name
Mus musculus
sex
female
strain
C57BL6J