Sample 8
Mus musculus
Protocols: Whole-blood clots were collected from DSS-treated C57BL/6J mice on day 8 of the experiment, corresponding to the recovery phase following 4 days of 2.5% (w/v) dextran sulfate sodium (DSS) administration and 4 days of treatment with either autoclaved water (control) or Shear-Labile Interaction Polymers (SLIPs, 6.66 mg/mL in drinking water). Blood was collected at euthanasia by cardiac puncture, transferred into RNase-free microcentrifuge tubes, allowed to clot at room temperature, and flash frozen in liquid nitrogen before storage at −80 °C. Total RNA was extracted from frozen blood clots using a modified TRIzol LS protocol adapted from the Genomic Medicine Biorepository method for RNA isolation from human peripheral blood. Briefly, clots were homogenized in 1× RBC lysis buffer (NH₄Cl/KHCO₃/EDTA) using a bead mill homogenizer, followed by TRIzol LS extraction and acid–phenol phase separation. After isopropanol precipitation and ethanol washing, an additional reprecipitation step with 3 M sodium acetate (pH 5.2) and 100% ethanol was performed to remove residual phenol. RNA was resuspended in nuclease-free water, quantified using the Qubit RNA HS Assay, and integrity assessed with an Agilent 4200 TapeStation. RNA libraries were prepared by the University of Florida Interdisciplinary Center for Biotechnology Research (ICBR).
ENA first public
2026-04-01
INSDC center name
UF
INSDC status
public
Submitter Id
E-MTAB-16067:Sample 8
age
8
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
disease
DSS (2.5% for 4 days) + SLIPs recovery
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blood
sample name
E-MTAB-16067:Sample 8
scientific_name
Mus musculus
sex
male
strain
C57BL/6J