Fecal sample from sooty mangabey BAK, Ta� National Park, 2022-12-06, for 16S metabarcoding
feces metagenome
Fecal sample collected from a sooty mangabey (Cercocebus atys) named BAK in Ta� National Park, C�te d'Ivoire, on 2022-12-06. Sample used for dietary analysis via 16S mitocondrial DNA metabarcoding.
ENA first public
2025-07-24
INSDC center name
HIOH
INSDC status
public
Submitter Id
Metabarcoding_FecesBAK_CA_TNP_20221206_Rep2
adapters
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
biological status
wild
broad-scale environmental context
tropical rainforest biome (ENVO:01000228)
collection date
2022-12-06
environmental medium
forest soil (ENVO:00002261)
geographic location (country and/or sea)
Cote d'Ivoire
geographic location (latitude)
not collected
geographic location (longitude)
not collected
geographic location (region and locality)
Cote d'Ivoire: Tai National Park
host body habitat
gastrointestinal tract
host body product
stool
host body site
feces
host common name
Sooty mangabey
host diet
omnivore
host disease status
Asymptomatic MPXV+
host life stage
adult
host scientific name
Cercocebus atys atys
host sex
male
host subject id
BAK
host taxid
1592215
library construction method
Tagsteady protocol with dual-indexed (P5 and P7) Illumina Y-adapters (https://doi.org/10.1111/1755-0998.13227)
library reads sequenced
15.151
library size
279
local environmental context
forest floor (ENVO:01001582)
multiplex identifiers
GTACAG : ACTAGC
nucleic acid amplification
Tagged conventional PCR (https://doi.org/10.1371/journal.pone.0000197 - modificactions indicated in PCR conditions and primers)
nucleic acid extraction
GeneMATRIX Stool DNA Purification Kit (Roboklon, Poland) - 40mg sample
pcr conditions
95degC_10min, 35 cycles of 95degC_12s, 59degC_30s, 70degC_25s, and 72degC_7min
pcr primers
16Smam1 5'-CGGTTGGGGTGACCTCGGA-3' and 16S mam2 5'-GCTGTTATCCCTAGGGTAACT-3'
project name
Fire-footed rope squirrels (Funisciurus pyrropus) are a reservoir host of monkeypox virus (Orthopoxvirus monkeypox)
sample capture status
active surveillance not initiated by an outbreak
sample collection device
plastic spatula and 2 mL cryotube
sample collection method
Faecal sample was collected from the ground right after defecation occurred with a plastic spatula and stored in a 2 mL cryotube
sample name
Metabarcoding_FecesBAK_CA_TNP_20221206_Rep2
sample storage duration
P2Y
sample storage temperature
-80
scientific_name
feces metagenome
source material identifiers
BAK_feces_20221206
target gene
16S mitochondrial DNA
target subfragment
130 bp fragment of 16S mitochondrial DNA