95928_1000_1
Lupinus angustifolius
Protocols: The immobilized Halo-tagged allele-specific RAP2-7 protein was incubated with the adapter-ligated DNA library. After incubation, the beads were washed, and the bound DNA was recovered, PCR-amplified, purified, and quantified. Negative controls were processed in parallel using adapter-ligated DNA libraries incubated with Magne HaloTag beads in the absence of recombinant protein. For more details please see accompanying manuscript.  Plants were cultivated in a growth chamber (16 h photoperiod, 22/18 °C day/night, 55–60% relative humidity, until they reached whole plant flowering stage. High-molecular-weight genomic DNA for DAP-seq was extracted from frozen young leaves (3 g) using a phenol:chloroform:isoamyl alcohol protocol, and quantified fluorometrically using the Qubit dsDNA Broad Range Assay (Thermo Fisher Scientific). Genomic DNA libraries were prepared according to Bartlett et al. (2017; doi.org/10.1038/nprot.2017.055), with minor modifications. For more details please see accompanying manuscript.
ENA first public
2026-06-30
INSDC center name
Institute of Plant Genetics, Polish Academy of Sciences
INSDC status
public
Submitter Id
E-MTAB-16430:95928_1000_1
age
52
broker name
ArrayExpress
collection date
not collected
common name
narrow-leaved blue lupine
cultivar
Bryanskij-237/83
developmental stage
whole plant flowering stage
genotype
Bryanskij-237/83
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
leaf
phenotype
alternative low-alkaloid
replicate
1
sample name
E-MTAB-16430:95928_1000_1
scientific_name
Lupinus angustifolius
strain
Lupinus angustifolius subsp. Angustifolius