C32L
Sus scrofa
Protocols: The pigs were on a standard basal diet for 7 days of adaptation prior to assigning to the dietary treatments. All herbal dietary treatments were provided to the pigs starting from the age of 42 days, except the AFB1 supplementation was administered from the age of 56 days on until the end of experiment at 70 days old. The pigs were slaughtered and the liver samples were collected, snap-frozen in liquid nitrogen and kept at -80 °C until the analysis. Total RNA was extracted from pig liver tissue using the TRIzol™ Reagent (Thermo Fisher Scientific), following the manufacturer's protocol. Approximately 50 mg of frozen liver tissue was homogenized in 1 mL of TRIzol™ using a tissue homogenizer. Phase separation was achieved by adding chloroform, followed by centrifugation. The aqueous phase containing RNA was precipitated with isopropanol and washed with 75% ethanol. The RNA pellet was then air-dried and resuspended in RNase-free water. RNA concentration was determined using NanoDrop 2000 spectrophotometer. RNA integrity was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples with RNA Integrity Number (RIN) values greater than 7 were selected for downstream applications. For miRNA sequencing, one microgram of high-quality total RNA (RIN > 7) was used for library preparation using the TruSeq Small RNA Library Preparation Kit (Illumina), according to the manufacturer's protocol. The workflow includes 3' and 5' adapter ligation, reverse transcription, and PCR amplification. The amplified products were subjected to size selection and purification using the BluePippin system (Sage Science) to isolate fragments in the range of ~145–160 bp, corresponding to miRNA inserts with adapters. The concentration of the purified libraries was quantified using the Qubit™ RNA HS (High Sensitivity) Assay Kit. Final library quality and fragment size distribution were evaluated on an Agilent Bioanalyzer using the Agilent High Sensitivity DNA Kit to ensure the presence of correctly sized and distinct library peaks prior to sequencing.
ENA first public
2026-06-30
INSDC center name
Research Institute for Farm Animal Biology (FBN) Physiological Genomics Unit
INSDC status
public
Submitter Id
E-MTAB-15396:C32L
age
70
broker name
ArrayExpress
collection date
not collected
common name
pig
developmental stage
young adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
N/A
isolate
not applicable
organism part
liver
sample name
E-MTAB-15396:C32L
scientific_name
Sus scrofa
sex
female
strain
crossbred TN70 (Norsvin Landrace x Large White)