Extraction negative control (PBS), 2024-12-10
blank sample
Extraction negative control consisting of PBS, processed alongside faecal samples to monitor for contamination during DNA extraction on 2024-12-10
ENA first public
2025-07-24
INSDC center name
HIOH
INSDC status
public
Submitter Id
ExtractionNegativeControl_20241210_Rep2
adapters
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
biological status
natural
broad-scale environmental context
laboratory environment (ENVO:01001405)
collection date
2024-12-10
environmental medium
laboratory environment (ENVO:01001405)
geographic location (country and/or sea)
Germany
geographic location (latitude)
not collected
geographic location (longitude)
not collected
geographic location (region and locality)
not applicable
host body habitat
not applicable
host body product
not applicable
host body site
not applicable
host common name
not applicable
host diet
not applicable
host disease status
not applicable
host life stage
not applicable
host scientific name
not applicable
host sex
not applicable
host subject id
not applicable
host taxid
2582415
library construction method
Tagsteady protocol with dual-indexed (P5 and P7) Illumina Y-adapters (https://doi.org/10.1111/1755-0998.13227)
library reads sequenced
15.151
library size
279
local environmental context
laboratory environment (ENVO:01001405)
multiplex identifiers
TCACTG : AGACCT
nucleic acid amplification
Tagged conventional PCR (https://doi.org/10.1371/journal.pone.0000197 - modificactions indicated in PCR conditions and primers)
nucleic acid extraction
GeneMATRIX Stool DNA Purification Kit (Roboklon, Poland) - 40mg sample
pcr conditions
95degC_10min, 35 cycles of 95degC_12s, 59degC_30s, 70degC_25s, and 72degC_7min
pcr primers
16Smam1 5'-CGGTTGGGGTGACCTCGGA-3' and 16S mam2 5'-GCTGTTATCCCTAGGGTAACT-3'
project name
Fire-footed rope squirrels (Funisciurus pyrropus) are a reservoir host of monkeypox virus (Orthopoxvirus monkeypox)
sample capture status
other
sample collection device
not applicable
sample collection method
not applicable
sample name
ExtractionNegativeControl_20241210_Rep2
sample storage duration
P0.5Y
sample storage temperature
4
scientific_name
blank sample
source material identifiers
not applicable
target gene
16S mitochondrial DNA
target subfragment
130 bp fragment of 16S mitochondrial DNA