Sample 60
Homo sapiens
Protocols: Whole blood samples were collected from participating individuals (n=149) in lithium heparin tubes and PBMCs were isolated according to standard protocols. The PBMCs were separated was using Ficoll‐Paque 1.077 g/mL density gradient centrifugation. Washed PBMCs were resuspended in freezing medium (DMSO, heat‐inactivated FCS, and RPMI). Cells were then transferred to a −80 C freezer and afterwards into liquid nitrogen the following day. Total mRNA was isolated from PBMCs using the RNeasy Mini Kit (Qiagen), and RNA levels were quantified by NanoDrop (Thermo Fisher Scientific). RNA integrity was confirmed using the Agilent 2100 BioAnalyzer (Agilent). Libraries were prepared with Illumina's Truseq stranded mRNA library prep kit with polyA enrichment.
ENA first public
2026-04-01
INSDC center name
Swiss Institute of Allergy and Asthma Research
INSDC status
public
Submitter Id
E-MTAB-16535:Sample 60
age
Child
broker name
ArrayExpress
collection date
not collected
common name
human
disease
Urban_Healthy
geographic location (country and/or sea)
not collected
individual
Co049LJ
isolate
not applicable
organism part
blood component
sample name
E-MTAB-16535:Sample 60
scientific_name
Homo sapiens