AM_IRF4_IL33_rep1
Mus musculus
Protocols: Alveolar macrophages were collected by bronchoalveolar lavage (BAL). BAL was lightly fixed with formaldehyde (final concentration of 0.1%) quenched with glycine and single-cell suspension was stained with monoclonal antibodies followed by cell sorting. Additional information can be found in original publication. CUT and RUN was carried out with minor adaptations from the protocol  described in the following reference:  \"Skene, P. J., Henikoff, J. G. & Henikoff, S. Targeted in situ genome-wide profiling with high efficiency for low cell numbers. Nat Protoc 13, 1006–1019 (2018)..\"  Additional information can be found in original publication. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturer's instructions. Cleanup of libraries was performed with AMPure XP beads (Beckman Coulter).
ENA first public
2025-12-13
INSDC center name
VIB-UGent Inflammation Research Center
INSDC status
public
Submitter Id
E-MTAB-16047:AM_IRF4_IL33_rep1
broker name
ArrayExpress
collection date
not collected
common name
house mouse
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lung
sample name
E-MTAB-16047:AM_IRF4_IL33_rep1
scientific_name
Mus musculus
sex
female
stimulus
IL33
strain
C57BL6J