Wild-type 1
Mus musculus
Protocols: Brains from male hemizygous 5XFAD (n=3) and wild-type control mice (n=3) aged 12 months were used. Brain tissue (excluding olfactory bulbs and cerebellum) was dissociated into a single cell suspension, and Fluorescence Activated Cell Sorting (FACS) was used to isolate CD31+, CD45- cells Following Fluorescence Activated Cell Sorting (FACS), total RNA was extracted from the isolated CD31+, CD45- brain endothelial cells. Nucleic acid isolation was performed according to the manufacturer's protocol. Single-cell gene expression libraries were generated using the 10x Genomics Chromium Next GEM Single Cell 5'Gene Expression, v2 Chemistry kit. Libraries were prepared according to the manufacturer's protocol, and raw data were later processed using Cell Ranger against the GRCm39-2024-A reference
ENA first public
2025-12-31
INSDC center name
Vanderbilt University
INSDC status
public
Submitter Id
E-MTAB-16239:Wild-type 1
age
12
broker name
ArrayExpress
cell type
endothelial cell
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
Wildtype 1
isolate
not applicable
organism part
brain
sample name
E-MTAB-16239:Wild-type 1
scientific_name
Mus musculus
sex
male
strain
5XFAD (C57BL/6J)