Sample 6
Homo sapiens
Protocols: Unstimulated saliva samples were collected in the Paxgene Saliva collectors. The subjects were instructed not to consume any food 30 minutes before the sample collection. The samples were stored at -80 °C for long-term use. 600 μL of saliva samples were centrifuged at 12000 x g for 2 mins to remove debris, and the clear liquid portion was processed further for RNA isolation. The small RNAs were isolated from the saliva samples using the Serum/Plasma Advanced Kit (Qiagen, cat. No. 217204).The RNA concentration and quality were assessed using the Qubit spectrophotometer.  Small RNA libraries were prepared from total RNA using the QIAseq miRNA Library Kit (QIAGEN) according to the manufacturer's recommendations. Briefly, 3′ and 5′ adapters were sequentially ligated to miRNA molecules followed by reverse transcription to synthesize cDNA incorporating unique molecular indices (UMIs) for bias correction. cDNA was then amplified by PCR using barcoded primers to enable sample multiplexing, and the resulting libraries were size-selected to enrich for miRNA-derived inserts. Final libraries were quantified, assessed for fragment size distribution, and sequenced on an Illumina platform to generate single-end reads compatible with downstream UMI-based deduplication and small RNA analysis.
ENA first public
2026-06-13
INSDC center name
Professor, Department of Biological Science, IISER Bhopal
INSDC status
public
Submitter Id
E-MTAB-16085:Sample 6
age
40
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
oral squamous cell carcinoma
disease staging
T4 tumor stage
geographic location (country and/or sea)
not collected
individual
S6
isolate
not applicable
organism part
saliva
sample name
E-MTAB-16085:Sample 6
scientific_name
Homo sapiens
sex
female