CKO-3-B
Mus musculus
Protocols: KLRG1+CD127- effector cells were sorted from OVA257-264-H-2Kb tetramer-binding CD8+ T cells isolated from Ch25hfl/fl and Ch25hfl/flCd4-Cre mice after infection with VSV-OVA. Cell sorting was performed on a BD FACSAria IIu flow cytometer. Following VSV-OVA infection, mice were euthanized via CO2 asphyxiation. Spleens were aseptically dissected. A single-cell suspension was prepared from the splenic tissue for subsequent staining and cell sorting. Mice were housed under a 12-hour light-dark cycle, at temperatures of 18–22°C, and at a humidity of 30–70%. Mice were infected intravenously with 1 × 10^6 plaque-forming units (PFU) of recombinant VSV-OVA. Image analysis and base calling were conducted by the MiSeq Control Software (MCS) or NovaSeq Control Software (NCS) on the MiSeq/NovaSeq instrument, converting the raw signal data into demultiplexed FASTQ files. Total RNA was extracted from the sorted KLRG1+CD127- effector cell samples using TRIzol reagent. Libraries were constructed from total RNA using a 5' RACE approach for reverse transcription, followed by TCR-specific PCR amplification. The PCR products were purified, end-repaired, and ligated to sequencing adapters. The final libraries were amplified with P5 and P7 primers, purified, and quality was assessed using a Qsep100 and Qubit 3.0 Fluorometer.
ENA first public
2026-04-01
INSDC center name
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Fifth People's Hospital, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China
INSDC status
public
Submitter Id
E-MTAB-16000:CKO-3-B
age
age at assessment
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Ch25hfl/flCd4-Cre
geographic location (country and/or sea)
not collected
individual
KLRG1+CD127–CD8+T cell
isolate
not applicable
organism part
spleen
sample name
E-MTAB-16000:CKO-3-B
scientific_name
Mus musculus
sex
male
strain
C57BL/6