Milddiabetespositive7
Mus musculus
Protocols: Mice were killed by cervical dislocation, pancreata were inflated with collagenase solution at 1 mg/ml, resected and placed in a water bath at 37°C for 10 min. After washing, islets were purified on a Histopaque-1119 / Histopaque-1083 gradient. Islets were cultured overnight at 37oC and 5% CO2 for respirometry, insulin secretion and enzyme studies, but were lysed ~3-4 hours after isolation for RNAseq analysis. Total RNA was isolated from islets using the ReliaPrep™ RNA Cell Miniprep System (Promega), according to the manufacturer's instructions. RNA quality was validated using an Agilent Bioanalyzer 2100. All samples had RIN values between 7.4 to 10.  500 ng of total RNA was used for library construction using Illumina's TruSeq Stranded mRNA prep kit following the manufacturer's instructions. Indexed libraries were purified and validated using an Agilent Bioanalyzer 2100. Sequencing libraries were pooled at equal molar concentration.
ENA first public
2025-12-31
INSDC center name
Institute of Metabolic Science - Metabolic Research Laboratories, University of Cambridge
INSDC status
public
Submitter Id
E-MTAB-16104:Milddiabetespositive7
age
14
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
diet
regular chow
genotype
RIPII-Cre-ER+/-,Kir6.2-V59Mfl/wt
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
islet of Langerhans
sample name
E-MTAB-16104:Milddiabetespositive7
scientific_name
Mus musculus
sex
male
strain
C57BL6/J