beta-lactam-resistant bacteria isolated from river waters site R1 in Kathmandu, Nepal
metagenome
0.2ml of the river water was seeded into CHROMagar ESBL plate and cultured overnight at 37 C. After incubation, 2 ml of PBS was added to CHROMagar ESBL plate, and all the grown colonies were collected. Of the collected bacterial suspension, 200 ?l was centrifuged and the pellet was resuspended in 1 ml of PBS. The 200 ?l of the resuspension solution was subjected to DNA extraction and metagenomic DNA was extracted.
sample_name
River water R1
collection_date
2024
geo_loc_name
Nepal
isolation_source
river water
lat_lon
missing