Sample 6
Mus musculus
Protocols: Organoids were cultured for 3 days prior to treatment with uroguanylin (1 micro molar) or medium alone. 18h following treatment, organoids were harvested in DMEM:f12 medium and RNA prepared. Organoids were cultured for 3 days prior to RNA extraction Organoids prepared from wild type and S839I mice were cultured for 3 days after passaging and then treated with uroguanylin for 16h. Total RNA purified form cultured organoids using Invitrogen RNA purification kits. RNA was treated with DNAse on the column. mRNA fragment and synthesis of cDNA followed by end repair and dA tailing. cDNA purified and amplified by PCR
ENA first public
2026-06-30
INSDC center name
Indian Institute of Science, Bangalore and Imperial College London
INSDC status
public
Submitter Id
E-MTAB-15862:Sample 6
age
8
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
S839I
geographic location (country and/or sea)
not collected
individual
organoid
isolate
not applicable
organism part
epithelium of small intestine
sample name
E-MTAB-15862:Sample 6
scientific_name
Mus musculus
sex
female
strain
C57BL6N