R2 plastic replicate 1 (barcode10)
aquatic metagenome
Plastic debris biofilm (plastisphere) sample collected at river estuary adjacent to a fish farm and community garden.
collection_date
2025-04-17
depth
0.5 m
env_broad_scale
freshwater biome [ENVO:00000873]
env_local_scale
estuary [ENVO:00000045]
env_medium
plastic [ENVO:01000404]
geo_loc_name
Israel: Taninim River, estuary (R2)
lat_lon
32.5386 N 34.9039 E
collection_method
collected by hand using nitrile gloves; plastic debris stored in a sterile bottle filled with sterile distilled water for transport to the laboratory
elev
0 m
misc_param
pH measured with portable pH meter; estuarine site; adjacent anthropogenic sources include a fish farm and community garden
ph
8
salinity
40 ppt
samp_collect_device
collected by hand using nitrile gloves; plastic debris stored in a sterile bottle filled with sterile distilled water for transport to the laboratory
samp_mat_process
biofilm recovered from plastic surface; cells lysed with lysozyme, SDS, and proteinase K; DNA extracted by phenol:chloroform:isoamyl alcohol (25:24:1) and precipitated with ethanol
samp_size
single plastic fragment per replicate
samp_vol_we_dna_ext
single plastic fragment per replicate
temp
22 C
biological_replicate
replicate 1
replicate_notes
independent biological replicate 1 of R2 plastic sample
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water