Viral-enriched metagenomic DNA from sewage sample
viral metagenome
1L raw hospital sewage was collected at UZ Ghent in April, 2023. The sample was stored at 4�C until further processing. Initial clarification was performed by centrifugation at 3 000 � g for 5 min at 4 �C, followed by a second centrifugation of the supernatant at either 3220 � g for 1 h at 4 �C. The resulting clear supernatant was filtered through a 0.45 �m polyethersulfone membrane. Virions were concentrated by polyethylene glycol (PEG) precipitation: NaCl was added to a final concentration of 1 M and dissolved with magnetic stirring, followed by centrifugation at 3 200 � g. PEG 8000 was then added to the supernatant to a final concentration of 10% (w/v), and samples were incubated on ice for 16 h. Precipitated material was recovered by centrifugation at either 7 196 � g for 40 min or 12 000�17 000 � g for 30 min, both at 4 �C. Pellets were resuspended in phosphate-buffered saline (137 mM NaCl, 2.7 mM KCl, 10 mM NaH2PO4, 1.8 mM KH2PO4).
ENA first public
2025-12-12
INSDC center name
UGent
INSDC status
public
Submitter Id
L4
collection date
2023-04-08
geographic location (country and/or sea)
Belgium
investigation type
metagenome
name of the sampling site
Universitair Ziekenhuis Gent Universitair (Corneel Heymanslaan 10, 9000 Ghent, Belgium)
sample name
L4
scientific_name
viral metagenome
sewage type
open sewer line