Liver_TOX_LFD_NRH_300_M_rep_1
Mus musculus
Protocols: Male and Female C57Bl/6NTac were purchased from Taconic. All mice were kept in a standard temperature- and humidity-controlled environment with a 12h:12h light-dark cycle. Mice had nesting material and ad libitum access to water and commercial diets. For the studies in Figures 1-3, mice were fed a regular mouse housing diet (Safe 150). For the high-fat diet study, we used D12492 from Research Diets, while using D12450J as a sucrose matched control. NRH was provided to mice in the drinking water, at a concentration of 0.1% of 0.4%, which approximately leads to a dose of 100 mg/kg*day or 400 mg/kg*day assuming mice drink around 3 ml per day and weight around 30 grams. Water bottles were renewed twice a week with a fresh solution. For the high-fat diet, the dose of NRH in the water bottles was adjusted weekly according to the average body weight of the cage. All animal experiments were carried out according to national Swiss and EU ethical guidelines and approved by the local animal experimentation committee under licenses VD2770.1, VD3647 and VD3841.  Animals were sacrificed after a 2 h fast, in order to stabilize systemic parameters and to allow the measurement of blood biochemistry in the fasting state. Tissues were collected upon sacrifice and flash-frozen in liquid nitrogen. Total mRNAs were extracted as described previously (Ratajczak, J. et al. Nat Commun, 2016) BRB-seq libraries were generated using a MERCURIUS BRB-seq kit (Alithea Genomics, #10813) according to the manufacturers instructions
ENA first public
2025-12-31
INSDC center name
Ecole Polytechnique Federale de Lausanne (EPFL)
INSDC status
public
Submitter Id
E-MTAB-14195_2:Liver_TOX_LFD_NRH_300_M_rep_1
age
8
alternate name
NAD-300-L-1
barcode
A04
barcode sequence
CATGCTAGCGATCG
batch
20250417
brbseq barcode kit
96 V5D Odt
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
diet
low fat diet
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
library
20250417_BRBseq_Liver_TOX
organism part
liver
sample name
E-MTAB-14195_2:Liver_TOX_LFD_NRH_300_M_rep_1
scientific_name
Mus musculus
sex
male
strain
C57Bl/6NTac
study
Toxicity study
tissue
Liver
treatment
NRH300
type
Demultiplexed sample