Sample 2
Homo sapiens
Protocols: Premortem cardiac samples were collected from the left ventricle (LV) right ventricle (RV) or right atrium (RA) within 15 minutes via snap freezing in liquid nitrogen Approximately 50mg of tissue/sample was disrupted using a metal bead (Qiagen #69989) in a 2mL tubes with 500uL TRIzol (Ambion #15596018) using the TissueLyser LT (Qiagen) and RNA extraction performed according to TRIzol manufacturer's instructions. DNA removal was performed using Qiagen RNAse-Free DNase kit (Qiagen #79254) and purified using the RNeasy Mini kit (Qiagen, #74104) according to manufacturer's instructions. RNA quality was evaluated using a Nanodrop and an RNA Nano Chip (Agilent #5067-1511) on an Agilent Bioanalyzer. Libraries were prepared with Illumina Stranded Total RNA prep Ligation with Ribo Zero Plus kit, according to manufacturer's instructions. Libraries were sequenced using a paired-end 250bp Illumina NovaSeq 6000 S4 flow cell.  Approximately 50mg of tissue/sample was disrupted using a metal bead (Qiagen #69989) in a 2mL tubes with 500uL TRIzol (Ambion #15596018) using the TissueLyser LT (Qiagen) and RNA extraction performed according to TRIzol manufacturer's instructions. DNA removal was performed using Qiagen RNAse-Free DNase kit (Qiagen #79254) and purified using the RNeasy Mini kit (Qiagen, #74104) according to manufacturer's instructions. RNA quality was evaluated using a Nanodrop and an RNA Nano Chip (Agilent #5067-1511) on an Agilent Bioanalyzer. Libraries were prepared with Illumina Stranded Total RNA prep Ligation with Ribo Zero Plus kit, according to manufacturer's instructions. Libraries were sequenced using a paired-end 250bp Illumina NovaSeq 6000 S4 flow cell.
ENA first public
2025-12-31
INSDC center name
USYD
INSDC status
public
Submitter Id
E-MTAB-16453:Sample 2
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
not available
disease
myocardial infarction
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
heart
sample name
E-MTAB-16453:Sample 2
sampling site
MI RV 1
scientific_name
Homo sapiens