mESCs-72h 20uM CHIR
Mus musculus
Protocols: RNA for bulk RNA sequencing was extracted (Qiagen RNeasy Mini Kit) from pooled mouse gastruloids according to their developmental stage: full 96-well plate if at 48h, 60h and 72h, half a 96-well plate if at 96h and one-third of a 96-well plate if at 120h or 144h. At least 3 replicate samples were obtained per condition, from several independent experiments. RNA was also extracted from 2D cultures of ESCs and EpiLSCs (T=0h) following the same procedure.  Library preparation was done by the IGC/GIMM Genomics facility in Portugal.
ENA first public
2026-04-01
INSDC center name
Universitat Pompeu Fabra
INSDC status
public
Submitter Id
E-MTAB-15149:mESCs-72h 20uM CHIR
broker name
ArrayExpress
cell line
ES-E14TG2a
cell type
ESCs
collection date
not collected
common name
house mouse
developmental stage
embryo
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
inner cell mass
sample name
E-MTAB-15149:mESCs-72h 20uM CHIR
scientific_name
Mus musculus
strain
129/Ola
time
72h