Sample 3
Homo sapiens
Protocols: For dissociation, spheroids were collected and centrifuged at 200g for 4 minutes and resuspended in a dissociation mix of  0.125 mg/mL Collagenase (Merck, C9891), 1 U/mL Dispase II (Thermo Fisher Scientific, #17105041) and 0.1 U/μL DNase (Merck, D4527) mixed in DMEM/F12 (Dulbecco's Modified Eagle's Media: Nutrient Mixture F-12; Thermo Fisher Scientific, #11330-057) for 20 minutes at 37°C on a DLAB MX-T6-Pro Roller at 10 RPM. Dissociation mix was neutralised using 1:3 dilution with basal media and centrifuged at 200 RCF for 5 minutes. Spheroids were resuspended in dissociation mix consisting of 0.05% trypsin-EDTA (Gibco, #25300062) and 0.1 U/μL DNase and placed at 37°C for 10-15 minutes, resuspending manually every 3 minutes. Dissociation mix was neutralised using 10% foetal bovine serum solution in DMEM/F12 at 1:3 dilution. Cells were centrifuged at 300 RCF for 5 minutes, and then counted. RNA extraction was done following RNeasy Mini kit (Qiagen, #74106) instructions. Samples were outsourced to GENEWIZ for nucleic acid library construction, and processed for ribosomal RNA depletion, and library preparation using standard NEB kit from New England Biolabs.
ENA first public
2026-04-01
INSDC center name
UON
INSDC status
public
Submitter Id
E-MTAB-15034:Sample 3
broker name
ArrayExpress
cell type
lung progenitor cell
collection date
not collected
common name
human
disease
normal
geographic location (country and/or sea)
not collected
growth condition
Lung Progenitor Day 2
isolate
not applicable
progenitor cell type
induced pluripotent stem cell
sample name
E-MTAB-15034:Sample 3
scientific_name
Homo sapiens