D5 DMSO
Homo sapiens
Protocols: On the days of the extraction, the cells were washed with D-PBS in the 24 well plate. Then the cells were lysed using RLT buffer + 1% b-mercaptoethanol, at RT, mixing well.  PIEZO1 activation using Yoda1  Macrophages were differentiated from CD14 monocytes (from 6 healthy donors) as described for 6 days in complete RPMI supplemented with 20 ng/mL M-CSF, changing medium on day 3. On day 5 of differentiation, the cells were stimulated with 20 uM Yoda1, for 12 hours (overnight), before being lysed for RNA extraction.  RNA was extracted using RNeasy Microkit, following manufacture's instructions (Qiagen, 74004). Libraries were prepared by University of Glasgow, MVLS Shared Research Facility, for Illumina sequencing.
ENA first public
2025-10-31
INSDC center name
School of infection and Immunity, University of Glasgow
INSDC status
public
Submitter Id
E-MTAB-15203:D5 DMSO
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
geographic location (country and/or sea)
not collected
growth condition
Cell culture monolayer
isolate
not applicable
sample name
E-MTAB-15203:D5 DMSO
scientific_name
Homo sapiens
sex
male
stimulus
DMSO