Primary_tumor_GEX
Homo sapiens
Protocols: Human liver tumor samples were collected during surgical resection (primary and recurrent lesions). Sections (5 µm) were mounted onto 10x Genomics Visium slides (11 mm) using CytAssist, H&E stained, imaged, and stored at −80 °C. De-identified metadata (age, sex) were retained. No bulk extraction was performed. Poly-A RNA was captured in situ according to the 10x Genomics Visium manufacturer's instructions. For protein, oligo-conjugated antibodies were applied following the CytAssist Spatial Protein procedure, and excess reagents were removed by the recommended washes. Visium gene expression (GEX) libraries and Feature Barcode antibody-derived tag (ADT) libraries were prepared with 10x Genomics kits following the manufacturer's protocols. Libraries were quantified (Qubit) and quality-checked (Bioanalyzer/Tapestation) before pooling.
ENA first public
2026-04-01
INSDC center name
Kyushu University Beppu Hospital
INSDC status
public
Submitter Id
E-MTAB-15756:Primary_tumor_GEX
age
61
broker name
ArrayExpress
clinical history
primary tumor at liver transplantation
collection date
not collected
common name
human
developmental stage
adult
disease
hepatocellular carcinoma
geographic location (country and/or sea)
not collected
individual
PT001
isolate
not applicable
organism part
liver
sample name
E-MTAB-15756:Primary_tumor_GEX
scientific_name
Homo sapiens
sex
male