Sample 8
Homo sapiens
Protocols: 501Mel CTRL, MCT1(sg7) and MCT1(KO) cells were maintained at 37°C and 5% CO2 in humidified air and grown in RPMI-1640 culture medium supplemented with 10% FBS and 1% penicillin–streptomycin. Cells were grown in T175 cell culture flasks. All 3 cell lines were treated or not with 17μM SIS3 over 2 weeks. Cells were split with 2/5 ratio after every 2-3 days at 90% confluency. All steps of growth protocol, treatment protocol and sample collection protocol were performed within the Inserm U1242 Unit RNA was extracted from 100M cells from at least 3 replicates of each condition using NucleoSpin RNA kit (Macherey-Nagel).  Nucleic acid library construction was performed with direct RNA sequencing kit (SQK-RNA002). All steps of nucleic acid library construction protocol were performed within the IGDRion platform (CNRS, IGDR UMR 6290, IGDRion, University of Rennes, Rennes, France).
ENA first public
2026-04-01
INSDC center name
INSERM, OSS (Oncogenesis Stress Signaling), UMR_S 1242, CLCC Eugene Marquis, Univ Rennes 1, 35000 Rennes
INSDC status
public
Submitter Id
E-MTAB-15088:Sample 8
broker name
ArrayExpress
cell line
BTO_0002956
cell type
melanocyte
collection date
not collected
common name
human
developmental stage
adult
disease
melanoma
genotype
BRAFv600e
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
skin
sample name
E-MTAB-15088:Sample 8
scientific_name
Homo sapiens