WT FGSCs，RNA-seq，rep2
Mus musculus
Protocols: FGSCs were cultured on STO feeder cells using Minimum Essential Medium-ɑ, supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 1 mM non-essential amino acid , 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 10 ng/mL mouse epidermal growth factor , 10 ng/mL mouse leukemia inhibitory factor, 40 ng/mL mouse glial cell line-derived neurotrophic factor, 10 ng/mL human basic fibroblast growth factor, and 1% penicillin-streptomycin. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected. Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer's protocols. mRNA was purified by NEBNext Poly(A) mRNA Magnetic Isolation Beads (NEB, USA). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).
ENA first public
2025-07-26
INSDC center name
SJTU
INSDC status
public
Submitter Id
E-MTAB-14257:WT FGSCs，RNA-seq，rep2
broker name
ArrayExpress
cell_line
FGSC
cell_type
germ line stem cell
collection date
not collected
common name
house mouse
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
ovary
replicate
rep2
sample name
E-MTAB-14257:WT FGSCs，RNA-seq，rep2
scientific_name
Mus musculus