Fecal sample from sooty mangabey GIS, Ta� National Park, 2022-12-31, for 16S metabarcoding
feces metagenome
Fecal sample collected from a sooty mangabey (Cercocebus atys) named GIS in Ta� National Park, C�te d'Ivoire, on 2022-12-31. Sample used for dietary analysis via 16S mitocondrial DNA metabarcoding.
ENA first public
2025-07-24
INSDC center name
HIOH
INSDC status
public
Submitter Id
Metabarcoding_FecesGIS_CA_TNP_202212231_Rep3
adapters
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
biological status
wild
broad-scale environmental context
tropical rainforest biome (ENVO:01000228)
collection date
2022-12-31
environmental medium
forest soil (ENVO:00002261)
geographic location (country and/or sea)
Cote d'Ivoire
geographic location (latitude)
not collected
geographic location (longitude)
not collected
geographic location (region and locality)
Cote d'Ivoire: Tai National Park
host body habitat
gastrointestinal tract
host body product
stool
host body site
feces
host common name
Sooty mangabey
host diet
omnivore
host disease status
Healthy
host life stage
adult
host scientific name
Cercocebus atys atys
host sex
female
host subject id
GIS
host taxid
1592215
library construction method
Tagsteady protocol with dual-indexed (P5 and P7) Illumina Y-adapters (https://doi.org/10.1111/1755-0998.13227)
library reads sequenced
15.151
library size
279
local environmental context
forest floor (ENVO:01001582)
multiplex identifiers
ATCGAG : GCATGA
nucleic acid amplification
Tagged conventional PCR (https://doi.org/10.1371/journal.pone.0000197 - modificactions indicated in PCR conditions and primers)
nucleic acid extraction
GeneMATRIX Stool DNA Purification Kit (Roboklon, Poland) - 40mg sample
pcr conditions
95degC_10min, 35 cycles of 95degC_12s, 59degC_30s, 70degC_25s, and 72degC_7min
pcr primers
16Smam1 5'-CGGTTGGGGTGACCTCGGA-3' and 16S mam2 5'-GCTGTTATCCCTAGGGTAACT-3'
project name
Fire-footed rope squirrels (Funisciurus pyrropus) are a reservoir host of monkeypox virus (Orthopoxvirus monkeypox)
sample capture status
active surveillance not initiated by an outbreak
sample collection device
plastic spatula and 2 mL cryotube
sample collection method
Faecal sample was collected from the ground right after defecation occurred with a plastic spatula and stored in a 2 mL cryotube
sample name
Metabarcoding_FecesGIS_CA_TNP_202212231_Rep3
sample storage duration
P2Y
sample storage temperature
-80
scientific_name
feces metagenome
source material identifiers
GIS_feces_202212231
target gene
16S mitochondrial DNA
target subfragment
130 bp fragment of 16S mitochondrial DNA