EPS_681
Homo sapiens
Protocols: Samples were collected in RNA Lysis buffer from Monarch® Total RNA Miniprep Kit according to the manufacturer's guidelines. RNA Extraction conducted using Monarch® Total RNA Miniprep Kit according to manufactures protocols Human mRNA Sequencing (WOBI) conducted by Novogene. mRNA library preparation (poly A enrichment). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.
ENA first public
2026-06-30
INSDC center name
Coventry University
INSDC status
public
Submitter Id
E-MTAB-17054:EPS_681
broker name
ArrayExpress
cell line
LHCN-M2
cell type
skeletal muscle myoblast
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-17054:EPS_681
scientific_name
Homo sapiens
stimulus
Stimulation