Exp1_WT_lung_Steady State
Mus musculus
Protocols: Exp1: 4 untreated C57BL6j mice were sacrificed and lung single cell suspensions were created. The cells were incubated with fluorophore labeled antibodies for FACS and TotalSeq C library for 30 minutes on 4°C. 30000 CD45-, 15000 CD45+CD64+ and 15000 CD45+CD64- cells per biological replicate were FACS sorted. Cells were pooled in the same eppendorf tube, diluted into a final concentration of 2000 cells/ μl and loaded on the 10X chromium device. The DNA libraries were prepared using the GemCode Single Cell 5′ Gel Bead and Library kit, version Next GEM v2 according to the manufacturer's instructions (10x Genomics, User Guide CG000330). Size selection with SPRIselect Reagent Kit (Beckman Coulter, B23318) was used to separate amplified cDNA molecules for 5′ gene expression and cell surface protein construction. The cDNA content of pre-fragmentation and post-sample index PCR samples was analyzed using the 2100 BioAnalyzer (Agilent).  The DNA libraries were prepared using the GemCode Single Cell 5′ Gel Bead and Library kit, version Next GEM v2 according to the manufacturer's instructions (10x Genomics, User Guide CG000330). Size selection with SPRIselect Reagent Kit (Beckman Coulter, B23318) was used to separate amplified cDNA molecules for 5′ gene expression and cell surface protein construction. The cDNA content of pre-fragmentation and post-sample index PCR samples was analyzed using the 2100 BioAnalyzer (Agilent).
ENA first public
2025-12-13
INSDC center name
VIB-UGent Inflammation Research Center
INSDC status
public
Submitter Id
E-MTAB-16099:Exp1_WT_lung_Steady State
broker name
ArrayExpress
cell type
immune and non-immune
collection date
not collected
common name
house mouse
disease
Steady state
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lung
sample name
E-MTAB-16099:Exp1_WT_lung_Steady State
scientific_name
Mus musculus
sex
female
strain
C57BL6J