HBMEC4-6h1T
Homo sapiens
Protocols: Samples were collected directly in the well using a lysing solution (1% 2-mercaptoethanol + Buffer RLT; RNeasy Mini Kit, 74106, Qiagen). Study 1: Human brain microvascular endothelial cells (HBMEC) and human dermal microvascular endothelial cells (HDMEC) were stimulated with either 0ng/mL, 1ng/mL or 10ng/mL of TNF for 0 hours, 10 minutes (0.167 hours), 30 minutes (0.5 hours), 2 hours, or 6 hours. Study 2: HBMEC were stimulated with either 1ng/mL or 10ng/mL of TNF for 0 hours, 6 hours, 12 hours, or 24 hours. RNeasy mini kit (Qiagen, 74106) buffers and protocol. RNA-sequencing libraries were prepared using the TruSeq Stranded mRNA kit (20020595, Illumina following the manufacturers protocol.
ENA first public
2025-10-31
INSDC center name
KAUST
INSDC status
public
Submitter Id
E-MTAB-14525:HBMEC4-6h1T
broker name
ArrayExpress
cell_line
human brain microvascular endothelial cell
cell_type
microvascular endothelial cell
collection date
not collected
common name
human
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
brain
sample name
E-MTAB-14525:HBMEC4-6h1T
scientific_name
Homo sapiens