Genomic DNA from Saccharina latissima strain SL-DOR-15_m1
Saccharina latissima
DNA was extracted from whole gametophyte cultures for ddRAD-seq using the Macherey Nagel Nucleospin plant II DNA plate extraction kit and the DNA was then subsequently cleaned with the Nucleospin gDNA XS clean-up kit.
Further Details
The sex of the strain was determined by PCR using the published (https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1201-7#Sec28) Saccharina latissima sex-locus-specific primers Sif2057Forward (ATGCGACTCCTGTCAATTCC), Sif2057 Reverse (CTGGGTTGGGTTTTCATCAC), Sjm4021 Forward (GGCTGTTTCAAGCATGGATT) and Sjm4021 Reverse (GCGTCGTCGTTACCTGATTT) .
INSDC center name
SBR
Submitter Id
SL-DOR-15_m1
collected_by
Phil Kerrison
collection date
2018-12-18
culture_collection
CCAP: 1318/160
geographic location (country and/or sea)
United Kingdom
geographic location (latitude)
50.56985
geographic location (longitude)
-2.427376
growth condition
Gametophytes grown as unialgal cultures (not axenic) in modified (half-strength) Provasoli-enriched seawater under red light at 13�C.
sample collection method
At the sampling site, cut four 1 cm2 pieces from the fertile part of the sporophyte blade (sorus), rinse with freshwater, wipe clean, place in a 50 ml Falcon tube sandwiched between two glass slides. In the laboratory, place the slides in a Petri dish in Provasoli-enriched (half-strength) natural seawater to grow the released meio-spores into gametophytes. Isolate individual gametophyte by micropipetting and grow in culture.
sample name
SL-DOR-15_m1
sample storage conditions
Frozen
sample storage temperature
-80
scientific_name
Saccharina latissima
strain
SL-DOR-15_m1