beta-lactam-resistant bacteria isolated from river waters near by downstream of Akashi-gawa, Japan
metagenome
0.2ml of the river water was seeded into CHROMagar ESBL plate and cultured overnight at 37 C. After incubation, 2 ml of PBS was added to CHROMagar ESBL plate, and all the grown colonies were collected. Of the collected bacterial suspension, 200 ?l was centrifuged and the pellet was resuspended in 1 ml of PBS. The 200 ?l of the resuspension solution was subjected to DNA extraction and metagenomic DNA was extracted.
sample_name
River water AKD
collection_date
2024
geo_loc_name
Japan
isolation_source
river water
lat_lon
missing