Positive Control (beef muscle), 2025-02-11
Bos taurus
Positive control consisting of german beef muscle previously bought in a german supermarket. PCR performed on 2025-02-11
ENA first public
2025-07-24
INSDC center name
HIOH
INSDC status
public
Submitter Id
PositiveControl_20250211_Rep3
adapters
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
biological status
breeder's line
broad-scale environmental context
laboratory environment (ENVO:01001405)
collection date
2025-02-11
common name
domestic cattle
environmental medium
laboratory environment (ENVO:01001405)
geographic location (country and/or sea)
Germany
geographic location (latitude)
not collected
geographic location (longitude)
not collected
geographic location (region and locality)
not applicable
host body habitat
skeletal muscle tissue
host body product
not applicable
host body site
skeletal muscle
host common name
Cattle
host diet
herbivore
host disease status
Healthy
host life stage
not provided
host scientific name
Bos taurus
host sex
not provided
host subject id
not provided
host taxid
9913
library construction method
Tagsteady protocol with dual-indexed (P5 and P7) Illumina Y-adapters (https://doi.org/10.1111/1755-0998.13227)
library reads sequenced
15.151
library size
279
local environmental context
laboratory environment (ENVO:01001405)
multiplex identifiers
AGTCTC : CAGGAT
nucleic acid amplification
Tagged conventional PCR (https://doi.org/10.1371/journal.pone.0000197 - modificactions indicated in PCR conditions and primers)
nucleic acid extraction
DNeasy Blood and Tissue kit (Qiagen, Germany) - 20mg sample
pcr conditions
95degC_10min, 35 cycles of 95degC_12s, 59degC_30s, 70degC_25s, and 72degC_7min
pcr primers
16Smam1 5'-CGGTTGGGGTGACCTCGGA-3' and 16S mam2 5'-GCTGTTATCCCTAGGGTAACT-3'
project name
Fire-footed rope squirrels (Funisciurus pyrropus) are a reservoir host of monkeypox virus (Orthopoxvirus monkeypox)
sample capture status
other
sample collection device
scalpel and tweezers
sample collection method
Positive control prepared using a small piece of raw beef muscle (Bos taurus) purchased from a supermarket in Germany
sample name
PositiveControl_20250211_Rep3
sample storage duration
P0.5Y
sample storage temperature
-20
scientific_name
Bos taurus
source material identifiers
not provided
target gene
16S mitochondrial DNA
target subfragment
130 bp fragment of 16S mitochondrial DNA