Sample 15
Danio rerio
Protocols: recently fertilized (0), 3 and 6 hours post fertilization embros were collected into RNA later Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) Ribo-off rRNA Depletion Kit（vazyme, Nanjing, China）was used to remove ribosomal RNA, then the libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.
ENA first public
2026-06-30
INSDC center name
CCMAR/CIMAR Centro de Ciências do Mar do Algarve
INSDC status
public
Submitter Id
E-MTAB-15099:Sample 15
age
6
broker name
ArrayExpress
collection date
not collected
common name
zebrafish
developmental stage
embryo stage
genotype
homozygous sst6 mutant
geographic location (country and/or sea)
not collected
individual
15
isolate
not applicable
organism part
whole embryo
sample name
E-MTAB-15099:Sample 15
scientific_name
Danio rerio
sex
unknown sex
strain
AB