Sample 37
Homo sapiens
Protocols: Transfections were carried out in 24-well plate for flow cytometry analysis.   H1299 cells were transfected with Lipofectamine® 3000 according to manufacturer's instructions and 300 ng of total DNA in 24-well plates. CHO and HEK293 were transfected with PEI transfection reagent with 300 ng of total DNA in 24-well plates. All cells were plated approximately 24h before transfection at 70000 to 80000 cells per well in 24-wells plates. At the moment of transfection, cells were put in starvation conditions, meaning in poor media for the first 24h. DNA was diluted in Opti-MEM reduced serum media (Gibco), before being mixed and incubated for 25 minutes prior to addition to the cells. All drugs were added 4h after transfection.  Modified-RNAs (modRNA) were transfected using Lipofectamine® 3000 protocol under modified conditions in which no P3000 reagent was used. RNA was first diluted in Opti-MEM reduced serum media (Gibco) and it was then mixed with diluted Lipofectamine reagent as manufacturer's instructions. The mix was incubated for 7 minutes prior to addition to the cells. The fast-forward protocol was used by seeding 120000 cells per well in 24-wells plates at the moment of transfection.  Libraries were prepared by Total RNAseq with ribo0 plus (Illumina)
ENA first public
2025-07-25
INSDC center name
Istituto Italiano di Tecnologia - IIT
INSDC status
public
Submitter Id
E-MTAB-15146:Sample 37
broker name
ArrayExpress
cell line
NCI-H1299
cell type
lung cancer cell
collection date
not collected
common name
human
developmental stage
adult
disease
non-small cell lung carcinoma
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lung
sample name
E-MTAB-15146:Sample 37
scientific_name
Homo sapiens