Sample 7
Homo sapiens
Protocols: For dissociation, spheroids were collected and centrifuged at 200g for 4 minutes and resuspended in a dissociation mix of  0.125 mg/mL Collagenase (Merck, C9891), 1 U/mL Dispase II (Thermo Fisher Scientific, #17105041) and 0.1 U/μL DNase (Merck, D4527) mixed in DMEM/F12 (Dulbecco's Modified Eagle's Media: Nutrient Mixture F-12; Thermo Fisher Scientific, #11330-057) for 20 minutes at 37°C on a DLAB MX-T6-Pro Roller at 10 RPM. Dissociation mix was neutralised using 1:3 dilution with basal media and centrifuged at 200 RCF for 5 minutes. Spheroids were resuspended in dissociation mix consisting of 0.05% trypsin-EDTA (Gibco, #25300062) and 0.1 U/μL DNase and placed at 37°C for 10-15 minutes, resuspending manually every 3 minutes. Dissociation mix was neutralised using 10% foetal bovine serum solution in DMEM/F12 at 1:3 dilution. Cells were centrifuged at 300 RCF for 5 minutes, and then counted. Nucleic acid extraction was obtained following Evercode WT v2.3 Parse Biosciences protocol. RT transcription was performed within each cell following single cell combinatorial barcoding. Following barcoding, cells were lysed and nucleic acid molecules extracted.  Single-cell RNA-seq libraries were generated using the Evercode WT v2.3 kit (Parse Biosciences). 8 sublibraries were generated.
ENA first public
2026-04-01
INSDC center name
UON
INSDC status
public
Submitter Id
E-MTAB-15033:Sample 7
broker name
ArrayExpress
cell type
alveolar type 2 cell
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
growth condition
3D Spheroids
injury
none
isolate
not applicable
replicate
Adult Alveolar 1.7
sample name
E-MTAB-15033:Sample 7
scientific_name
Homo sapiens