Sample 88
Homo sapiens
Protocols: Transfections were carried out in 24-well plate for flow cytometry analysis.   H1299 cells were transfected with Lipofectamine® 3000 according to manufacturer's instructions and 300 ng of total DNA in 24-well plates. CHO and HEK293 were transfected with PEI transfection reagent with 300 ng of total DNA in 24-well plates. All cells were plated approximately 24h before transfection at 70000 to 80000 cells per well in 24-wells plates. At the moment of transfection, cells were put in starvation conditions, meaning in poor media for the first 24h. DNA was diluted in Opti-MEM reduced serum media (Gibco), before being mixed and incubated for 25 minutes prior to addition to the cells. All drugs were added 4h after transfection.  Modified-RNAs (modRNA) were transfected using Lipofectamine® 3000 protocol under modified conditions in which no P3000 reagent was used. RNA was first diluted in Opti-MEM reduced serum media (Gibco) and it was then mixed with diluted Lipofectamine reagent as manufacturer's instructions. The mix was incubated for 7 minutes prior to addition to the cells. The fast-forward protocol was used by seeding 120000 cells per well in 24-wells plates at the moment of transfection.  Libraries were prepared by Total RNAseq with ribo0 plus (Illumina)
ENA first public
2025-07-25
INSDC center name
Istituto Italiano di Tecnologia - IIT
INSDC status
public
Submitter Id
E-MTAB-15146:Sample 88
broker name
ArrayExpress
cell line
HEK293T
cell type
embryonic cell
collection date
not collected
common name
human
developmental stage
embryo stage
disease
No disease related
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
kidney
sample name
E-MTAB-15146:Sample 88
scientific_name
Homo sapiens