HBE_MP1_rep1_MP7384
Homo sapiens
Protocols: 16HBE14O- were purchased and maintained using standard protocols. mRNA was converted to cDNA using Superscript III Reverse Transcriptase. cDNA was treated with RNAse A (invitrogen #12091-021) and RNAse T1 (ThermoFisher #EN0541) for one hour and then cleaned with the Qiagen Minelute PCR purification kit. After transfection, cells were lysed using a 20g needle and RNA was extracted using Qiagen RNeasy mini kit.  Libraries were amplified with 10-15 PCR cycles using Q5 Hot Start High-Fidelity 2x Master Mix (NEB #M0494S) and pooled before clean up using Agencourt AMPure XP beads (0.6x + 1.2x double cleanup; Beckman Coulter; #A63882).
ENA first public
2025-03-01
INSDC center name
University of Chicago
INSDC status
public
Submitter Id
E-MTAB-14273:HBE_MP1_rep1_MP7384
broker name
ArrayExpress
cell_line
16HBE14o-
cell_type
epithelial cell of bronchus
collection date
not collected
common name
human
disease
normal
experiment id
1
geographic location (country and/or sea)
not collected
individual
1
isolate
not applicable
organism part
lung
sample name
E-MTAB-14273:HBE_MP1_rep1_MP7384
sample type
RNA
scientific_name
Homo sapiens
sex
male