HTO1bv
Homo sapiens
Protocols: Entheseal biopsy of the extensor common tendon´s enthesis was performed. Briefly, biopsies were done by Blakesley forceps in 60° flexed position after identifying the lateral epicondyle, the olecranon and the radial head by ultrasound. Entheseal tissue slides from six patients before and after treatment with secukinumab were analyzed with GeoMX Digital Spatial Profiler (Nanostring) Tissue slides were prepared following the RNA Slide Preparation Protocol in the GeoMx NGS Slide Preparation User Manual (NanoString, MAN¬ 10150-06). Briefly, slides were baked at 60°C for 60 min and then treated sequentially with HIER (ER2, 20 min, 99°C) and 1 mg/mL proteinase K (Ambion, cat. 2546) in 1X phosphate-buffered saline (PBS) at 37°C for 15 min. After pretreatment, slides were hybridized with the GeoMx Whole Transcriptome Atlas Panel (GMX-RNA-NGS-HuWTA-4). The library preparation and purification were carried out using GeoMX DSP NGS Readout Protocol (MAN-10153-06).
ENA first public
2025-12-31
INSDC center name
Department of Medicine 3 – Rheumatology and Immunology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen
INSDC status
public
Submitter Id
E-MTAB-15134:HTO1bv
age
48
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
psoriatic arthritis
geographic location (country and/or sea)
not collected
individual
eBio_Pt_11
isolate
not applicable
organism part
enthesis
response to treatment
responder
roi
4232.78
sample name
E-MTAB-15134:HTO1bv
scientific_name
Homo sapiens
segment
Vimentin
sex
male
slide
3