Cell_shBoth_n3_35
Homo sapiens
Protocols: Cells were treated with trypsin, centrifuged and washed in PBS. After centrifugation, the cell pellet was frozen at -80°C until the RNA extraction process. HepG2 cell line were maintained at 37°C in a humidified 5% CO2 atmosphere in Dulbecco's modified Eagle's medium (DMEM: 4.5 g/l glucose, glutamax, pyruvate) supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin, and 100 μg/mL streptomycin. Total RNA extraction was performed using the Direct-zol RNA Microprep kit (Zymo Research, R2062). SmallRNA-seq libraries were prepared using the QIAseq miRNA Library kit (QIAGEN, 331502) and QIAseq miRNA 96 index Kit IL UDI-F (QIAGEN, 331665) according to manufacturer's protocol. Briefly, undiluted 3' adapters were first ligated on 100ng of total RNA. Then, undiluted 5' adapters were ligated and RNA retro-transcribed with undiluted RT primers to generate double strand cDNA. A final PCR of 16 cycles was performed to amplify libraries and barcode samples with unique dual indices. An additional purification step allowed to obtain 200-300 bp fragments.
ENA first public
2026-04-01
INSDC center name
University of Bordeaux, INSERM, Neurocentre Magendie, U1215, F-33000 Bordeaux, France
INSDC status
public
Submitter Id
E-MTAB-15638:Cell_shBoth_n3_35
broker name
ArrayExpress
cell line
HepG2
cell type
hepatocyte
collection date
not collected
common name
human
disease
liver cancer
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
liver
sample name
E-MTAB-15638:Cell_shBoth_n3_35
scientific_name
Homo sapiens