AD_REP2_S_09
Mus musculus
Protocols: Samples derive from MACS-isolated O4+ oligodendrocytes. RNA was isolated from oligodendrocytes using the RNA Micro Kit (Qiagen).  Libraries were prepared with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (#E6420S, New England Biolabs) and NEBNext Multiplex Oligos for Illumina (Index Primers 1 12) (#E7335S, New England Biolabs), following the corresponding instruction manual (Version5.0_5/20). The protocol started with 10 ng total RNA when available (otherwise, entire sample). Full-length cDNA was generated by template switching, followed by cDNA amplification and cleanup; 1 μl amplified cDNA was evaluated on an Agilent High Sensitivity DNA chip (#5067-4626, Agilent Technologies).
ENA first public
2025-12-31
INSDC center name
College de France
INSDC status
public
Submitter Id
E-MTAB-16400:AD_REP2_S_09
broker name
ArrayExpress
cell type
O4 Oligodendrocyte
collection date
not collected
common name
house mouse
developmental stage
Adult
disease
Alzheimer's disease
genotype
3xTg-AD
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
brain
sample name
E-MTAB-16400:AD_REP2_S_09
scientific_name
Mus musculus
sex
male
strain
C57BL/6