Exp1_WT-addback_IL33_1
Mus musculus
Protocols: Experiment 1:  WT or IRF4-/- alveolar macrophages (AMs) were culture expanded and transferred into depleted SPAM-deleter animals. After 1 week, all mice were subjected to IL-33 induced inflammation (3 consecutive doses of 100 ng intratracheally). AMs were harvested from bronchoalveolar lavage fluid 1 day after the final dose of IL-33 and FACS sorted into RLTplus buffer, supplemented with 1% βmercaptoethalol. Qiagen RNeasy Plos Micro kit following manufacturer's instructions Illumina stranded total RNA library prep kit was used following manufacturer's instruction
ENA first public
2025-12-13
INSDC center name
VIB-UGent Inflammation Research Center
INSDC status
public
Submitter Id
E-MTAB-15952:Exp1_WT-addback_IL33_1
broker name
ArrayExpress
collection date
not collected
common name
house mouse
genotype
CD45.1 wild type
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Alveolar macrophage
sample name
E-MTAB-15952:Exp1_WT-addback_IL33_1
scientific_name
Mus musculus
sex
female
stimulus
3 x IL-33 intratracheal
strain
SPAM-deleter