H1299-Con-03
Homo sapiens
Protocols: Cells were collected by Trypsization and washed with PBS twice.  All cells were cultured in RPMI medium supplemented with 10% FBS and 1% penicillin–streptomycin. After cell collection, total RNA was extracted using the RNeasy Kit (QIAGEN) according to the manufacturer's instructions. Approximately 2 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). Following purification, the poly(A) mRNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with a strand-specific library preparation by dUTP method. The average insert size for the paired-end libraries was 300±50 bp.
ENA first public
2026-02-28
INSDC center name
Emory University
INSDC status
public
Submitter Id
E-MTAB-16032:H1299-Con-03
broker name
ArrayExpress
cell line
H1299
cell type
lung cancer cell
collection date
not collected
common name
human
developmental stage
adult
disease
cancer
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
lung
sample name
E-MTAB-16032:H1299-Con-03
scientific_name
Homo sapiens