Scramble_2
Homo sapiens
Protocols: LUHMES cells were differentiated for 7 days. Cells were transfected with siRNA at day 3 and retrieved at day 7 of differentiation (siRNA Control or siRNA ORF1). Lund human mesencephalic cells (LUHMES) cells were differentiated for 7 days, using  Advanced DMEM/F12 added with 1% N-2 supplement and GlutaMax, supplemented with 1 µg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech, # 450-10) and 1mM cAMP (Sigma, # D0627). Cells were transfected with siRNA at day 3 and retrieved at day 7 of differentiation (siRNA Control or siRNA ORF1) RNA was harvested using Direct-zol™ RNA Microprep (Zymo, # R2062). Libraries including rRNA removal were generated using the RNA Library Prep Kit for Illumina following manufacturer's recommendations.
ENA first public
2026-01-01
INSDC center name
Centre Interdisciplinaire de Recherche en Biologie (CIRB) - Collège de France
INSDC status
public
Submitter Id
E-MTAB-15856:Scramble_2
broker name
ArrayExpress
cell line
LUHMES
cell type
dopaminergic neuron
collection date
not collected
common name
human
developmental stage
7 days differntiated LUHMES cells
geographic location (country and/or sea)
not collected
individual
biological replicat 2
isolate
not applicable
sample name
E-MTAB-15856:Scramble_2
scientific_name
Homo sapiens