Control_2
Mus musculus
Protocols: After mouse sacrifice hearts were excised and retrograde perfused with first 5ml Heparin/PBS solution followed by 1ml PBS. Perfused hearts were place in OCT, snap-frozen in pre-cooled isopentane (-30 to -40°C) and stored at -80°C until further use. 10µm thick tissue sections were cut in a cryostat at -20°C chamber temperature and -10°C object head temperature and placed at the 10X Visium Gene Expression slide. Prepared Visium slides were used as input for the Visium Spatial Gene Expression workflow (10X Genomics, Pleasanton, USA) according to the manufacturer's instructions. Tissue permeabilization was carried out for 12 minutes Library construction was performed to manufacturer's instructions, the Visium Spatial Gene Expression workflow (10X Genomics, Pleasanton, USA).
ENA first public
2026-02-28
INSDC center name
Instiute of Pharmacology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Germany
INSDC status
public
Submitter Id
E-MTAB-12700_2:Control_2
age
14
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
iAdipoQCre: wt, ATGL-flox: homo
geographic location (country and/or sea)
not collected
individual
350467
injury
ischemia reperfusion injury
isolate
not applicable
organism part
heart
sample name
E-MTAB-12700_2:Control_2
scientific_name
Mus musculus
sex
male
strain
B6.129-Pnpla2tm1Eek/J Tg(Adipoq-cre/ERT2)1Soff/J