Tsu-0 met1 mutant 03
Arabidopsis thaliana
DNA extracted from rosettes of a single 35-days-old individual of a CRISPR-induced homozygous met1 mutant of accession Tsu-0 (ecotype_ID: 7373)
ENA first public
2025-03-19
INSDC center name
MPI for Biology Tuebingen
INSDC status
public
Submitter Id
Tsu-0_met1_03
collection date
not applicable
common name
thale cress
geographic location (country and/or sea)
Japan
geographic location (latitude)
34.43
geographic location (longitude)
136.31
growth facility
growth chamber
isolation and growth condition
Seeds were germinated on soil and stratified in darkness at 4C for seven days, grown for 2 weeks in a growth chamber (CU-36L5; Percival Scientific, Wertingen, Germany) on half-strength MS agar plates supplemented with 1% w/v sucrose in long day conditions (16 h light) at 23C under 110 umol m-2 s-1, after which they were transferred to soil in long day conditions at 23C and 65% relative humidity under 110-140 umol m-2 s-1 light provided by Philips GreenPower TLED modules (Philips Lighting GmbH, Hamburg, Germany). To reduce starch accumulation, plants were placed into darkness for 24 h before harvesting.
number of replicons
5
plant developmental stage
vegetative
plant growth medium
half-strength MS agar + soil
plant structure
Rosettes
ploidy
diploid
sample health state
healthy
sample name
Tsu-0_met1_03
scientific_name
Arabidopsis thaliana
subspecific genetic lineage name
Tsu-0 met1
subspecific genetic lineage rank
ecotype