Sample 7
Klebsormidium nitens
Protocols: Samples were harvested via centrifugation, washed once in sterile MilliQ water, and snap-frozen in liquid nitrogen Cell suspensions were precultured in liquid Full Yamagami media for seven days prior to treatment Klebsormidium cultures were inoculated to liquid Full Yamagami media (pH5.5, 1% sucrose) containing the chemical of interest and grown for 48h at 80rpm, at 22°C, under 24h of low light. Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kit Directional mRNA library preparation (poly A enrichment)
ENA first public
2025-10-31
INSDC center name
Temasek Life Sciences Laboratory
INSDC status
public
Submitter Id
E-MTAB-15889:Sample 7
broker name
ArrayExpress
collection date
not collected
ecotype
NIES-2285
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
whole organism
replicate
1
sample name
E-MTAB-15889:Sample 7
scientific_name
Klebsormidium nitens
stimulus
5µM methionine sulfoxime treatment for 48h