seo6
Canis lupus familiaris
Protocols: A total of 24 beagle dogs aged 3 years (n = 8), 5 years (n = 8), and 10 years (n = 8) were investigated for age-related molecular signatures in their methylomes. All dogs were kept at the NIAS under standardized housing conditions that were maintained through continuous monitoring, vaccination, and anthelmintic treatment. The experiment was ethically approved by the Institutional Animal Care and Use Committee of the National Institute of Animal Science (NIAS 2022-586) and Gyeongsang National University (GNU-240802-D0152-01). Whole blood samples were collected using a 21-gauge needle and 5-mL syringe from the jugular vein of each dog. RNA from whole blood was extracted using a PAXgene Blood RNA Kit (762174; QIAGEN, Germantown, MD, USA) following the manufacturer's instructions.  Libraries were prepared for 150 bp paired-end sequencing using a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).
ENA first public
2026-01-01
INSDC center name
Gyeongsang National University Animal Genomics Laboratory
INSDC status
public
Submitter Id
E-MTAB-15574:seo6
age
3
broker name
ArrayExpress
collection date
not collected
common name
dog
developmental stage
adult
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blood
sample name
E-MTAB-15574:seo6
scientific_name
Canis lupus familiaris
sex
male