Sample 1
Homo sapiens
Protocols: HPAEpiCs on ALI day 28 were washed with PBS, incubated in 0.25% trypsin-EDTA, detached from the inserts, and separated by pipetting. Trypsinization was stopped by adding Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum. The cell suspension was then centrifuged, washed, and passed through a 40 µm Flowmi cell strainer (Bel-Art Products, Wayne, NJ, USA). The obtained cells were fixed with 4% formaldehyde in PBS, and subsequently treated with the Quenching Buffer and Enhancer contained in a Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (10x Genomics). Cell barcoding, amplification, and library construction were performed using a Chromium Fixed RNA Profiling Reagent Kit (10x Genomics) in accordance with the manufacturer's protocol. Isolated single cells were loaded onto a 10x Chromium X (10x Genomics), barcoded, and pre-amplified to construct the sequencing library.
ENA first public
2026-01-01
INSDC center name
Japan Tobacco Inc.
INSDC status
public
Submitter Id
E-MTAB-14782:Sample 1
age
20
broker name
ArrayExpress
cell_type
epithelial cell
collection date
not collected
common name
human
developmental stage
embryo stage
disease
normal
geographic location (country and/or sea)
not collected
individual
Donor 1
isolate
not applicable
organism part
alveolus of lung
sample name
E-MTAB-14782:Sample 1
scientific_name
Homo sapiens
sex
female