Sample 12
Mus musculus
Protocols: Cardiomyocyte-specific nuclei isolation was performed via a fluorescence labeling of PCM1 and fluorescence-activated nuclei sorting. PCM1-positive nuclei were subjected to RNA extraction based on the RNeasy Micro Kit (Qiagen). Animals received Tamoxifen for KO induction (75mg/kg BW/d) followed by an Isoprenaline treatment (30mg/kg BW/d) PCM1-positive nuclei were subjected to RNA extraction based on the RNeasy Micro Kit (Qiagen). Library construction was performed according to the SmartSeq2 protocol (Picelli, S., Faridani, O., Björklund, Å. et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc9, 171–181 (2014). https://doi.org/10.1038/nprot.2014.006 and Bianca P Hennig, Lars Velten, Ines Racke, Chelsea Szu Tu, Matthias Thoms, Vladimir Rybin, Hüseyin Besir, Kim Remans, Lars M Steinmetz, Large-Scale Low-Cost NGS Library Preparation Using a Robust Tn5 Purification and Tagmentation Protocol, G3 Genes|Genomes|Genetics, Volume 8, Issue 1, 1 January 2018, Pages 79–89, https://doi.org/10.1534/g3.117.300257)
ENA first public
2026-05-16
INSDC center name
Klaus Tschira Institute for Integrative Computational Cardiology, Heidelberg, Germany and DZHK (German Center for Cardiovascular Research), Partner Site Heidelberg/Mannheim, Germany
INSDC status
public
Submitter Id
E-MTAB-14396:Sample 12
age
14
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genetic modification
AVV9-Acadvl
genotype
Cre+ Prmt5-cKO
geographic location (country and/or sea)
not collected
individual
4288
isolate
not applicable
organism part
heart left ventricle
sample name
E-MTAB-14396:Sample 12
scientific_name
Mus musculus
sex
female
strain
C57BL/6N