Viral DNA metavirome from BLM1 deep-aquifer groundwater collected in 2021
viral metagenome
Virus-enriched groundwater sample collected in 2021 from the BLM1 deep continental aquifer in the central Amargosa Desert, California. Microbial cells were removed by 0.2-m filtration, and intact virions were concentrated by hollow-fiber ultrafiltration using a 29-kDa molecular-weight cutoff.
collection_date
2021-07-20
depth
695.4 m
env_broad_scale
freshwater biome [ENVO:00000873]
env_local_scale
aquifer [ENVO:00012408]
env_medium
groundwater [ENVO:01001004]
geo_loc_name
USA: California, Inyo County, central Amargosa Desert
lat_lon
36.4004 N 116.4692 W
alkalinity
433 mg/L as CaCO
alkalinity_method
Titration according to Standard Methods SM 2320 B-2011
calcium
33.8 mg/L
collection_method
Intact virions were collected from pumped groundwater over an 1824 h interval following upstream removal of microbial cells with a Spectrum MediaKap 50 filter and field-based hollow-fiber ultrafiltration using REXEED-25S ultrafilters with a 29-kDa molecular-weight cutoff.
conduc
1383 S/cm
diss_carb_dioxide
2.56 mM
diss_hydrogen
0.01 mM
diss_org_carb
0.001 mg/L
elev
695.4 m
isolation_source
Virus-enriched fraction concentrated from BLM1 deep-aquifer groundwater
magnesium
13.9 mg/L
nitrate
0.002 mg/L as N
nitrite
0.003 mg/L as N
nitro
3.46 mM
ph
7.5
redox_potential
-165.3 mV
samp_collect_device
Submersible pump (installed below the water table at ~70m b.l.s., ~30m below the static water table) connected to a gas-tight surface sampling manifold and a REXEED-25S hollow-fiber ultrafilter (29 kDa; Millipore), following upstream removal of microbial cells using a Spectrum MediaKap 50 filter.
samp_mat_process
Viral retentates were frozen on dry ice in the field, stored at 80 C, and further concentrated to approximately 100 mL in the laboratory using Centricon Plus-70 centrifugal filter cartridges (30 kDa; Millipore).
samp_size
approximately 100 mL of concentrated viral
samp_store_dur
1 week
samp_store_loc
Environmental Microbiology Laboratory, Desert Research Institute, Las Vegas, Nevada, USA
samp_store_temp
-80 C
samp_vol_we_dna_ext
Approximately 100 mL
silicate
31.7 mg/L as SiO2
size_frac
0-0.2 micrometer
size_frac_up
0.2 micrometer
sodium
212 mg/L
sulfate
169 mg/L
temp
54 C
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.water