Sample 5
Mus musculus
Protocols: In vivo studies conducted in accordance with the German Animal Welfare Act. Generation of Prmt5-cKO mice by mating Prmt5^loxP/loxP mice with alpha-MHC-MerCreMer transgenic mice, followed by Cre recombinase activation via Tamoxifen. Prmt5 genetic deletion induced at 8 weeks with Tamoxifen (75mg/kg BW/d) via oral gavage over two cycles. Post-Tamoxifen, a 3-week recovery period was kept before starting Isoprenaline treatment (30mg/kg BW/d) for 3 days followed by organ harvest. LV tissue RNA samples: Tissue was subjected to QIAzol-based RNA extraction according to manufacturer's protocols. LV tissue RNA samples: Library preparation and sequencing as 100 bp paired-end (BGISEQ-500) was performed by BGI, Hong Kong.
ENA first public
2026-05-16
External Id
SAMEA114785889
INSDC center name
Klaus Tschira Institute for Integrative Computational Cardiology, Heidelberg, Germany and DZHK (German Center for Cardiovascular Research), Partner Site Heidelberg/Mannheim, Germany
INSDC last update
2023-12-03T10:09:04Z
INSDC status
public
Submitter Id
E-MTAB-13594:Sample 5
age
13
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Cre+ Prmt5 wildtype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
heart left ventricle
sample name
E-MTAB-13594:Sample 5
scientific_name
Mus musculus
sex
female
strain
C57BL/6N