Sample 8
Homo sapiens
Protocols: sample preparation began with gel digestion of muscle constructs using Trizol (TR118 MRC), followed by phase separation with 1-Bromo-3-chloropropane (B9673). RNA extraction from muscle cells was then performed according to ReliaPrep™ RNA Miniprep Systems' protocol (Promega), which included DNase treatment. NEBNext UltraExpress RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.
ENA first public
2025-10-31
INSDC center name
Institute of Oncology Research
INSDC status
public
Submitter Id
E-MTAB-15933:Sample 8
broker name
ArrayExpress
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
myobundles
replicate
2
sample name
E-MTAB-15933:Sample 8
scientific_name
Homo sapiens
stimulus
Not stimulated
time point
D0