D1921H16
Homo sapiens
Protocols: LUHMES cells were differentiated for 7 days. Cells were transfected with siRNA at day 3 and retrieved at day 7 of differentiation (siRNA Control or siRNA ORF1). Lund human mesencephalic cells (LUHMES) cells were differentiated for 7 days, using  Advanced DMEM/F12 added with 1% N-2 supplement and GlutaMax, supplemented with 1 µg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech, # 450-10) and 1mM cAMP (Sigma, # D0627). Cells were transfected with siRNA at day 3 and retrieved at day 7 of differentiation (siRNA Control or siRNA ORF1) Nucleic acid extraction was performed using a commercial kit (Diagenode Cat. Cat. No.C01080001) according to the instructions of the manufacturer. ATAC-seq and library construction were performed using a commercial kit (Diagenode Cat. Cat. No.C01080001) according to the instructions of the manufacturer.
ENA first public
2026-01-01
INSDC center name
Centre Interdisciplinaire de Recherche en Biologie (CIRB) - Collège de France
INSDC status
public
Submitter Id
E-MTAB-15871:D1921H16
broker name
ArrayExpress
cell line
LUHMES
cell type
dopaminergic neuron
collection date
not collected
common name
human
differentiation
7 days differentiated LUHMES cells
geographic location (country and/or sea)
not collected
individual
biological replicate 2
isolate
not applicable
progenitor cell type
embryonic neuronal precursor cell
sample name
E-MTAB-15871:D1921H16
scientific_name
Homo sapiens