Saint-Livres T0 B
uncultured eukaryote
Saint-Livres bulk soil sampled with spatula at four different spots on a 20x20 cm area and 12 cm depth, sieved at 2mm
ENA first public
2026-01-01
INSDC center name
University of Neuchatel
INSDC status
public
Submitter Id
SL-2-B-T0
broad-scale environmental context
temperate deciduous broadleaf forest [ENVO:01000385]
collection date
2023-11-22
depth
0-0.1
elevation
670
environmental medium
soil [ENVO:00001998]|plant litter [ENVO:01000628]
experimental factor
SL T0 B
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.52275
geographic location (longitude)
6.38431
geographic location (region and locality)
Saint-Livres
local environmental context
soil surface layer [ENVO:04000010]|litter layer [ENVO:01000338]
mean annual precipitation
1181.6
mean annual temperature
9.1
multiplex identifiers
bc1004:CACGCACACACGCGCG; bc1054:GCGCACGCACTACAGA
nucleic acid extraction
DNAeasy PowerSoil Pro Kit, QIAGEN
pH
4.57
pH method
five grams of dry soil mixed in 12.5 ml of distilled water
pcr primers
616sfm1:TTAAAAMGCTCGTAGTYG; 616sfm2:TTAAAWCARTTATWRTYT; 28s3rm:ATTAAACWGTYRGATTCCC
project name
Fishing Active Soil Protists
sample name
SL-2-B-T0
sample volume or weight for DNA extraction
0.3
scientific_name
uncultured eukaryote
sieving
2 mm
slope aspect
341
slope gradient
4
target gene
18S; ITS; 28S
total organic carbon
3.42556476593018
total organic carbon method
Thermo ScientificTM FLASH 2000 CHNS elemental analyzer