U2OS_CC_PARGi_R3
Homo sapiens
Protocols: Extracted molecule: total RNA DMEM (Sigma) supplemented with 10% FBS (Gibco) and penicillin-streptomycin (100 U/mL, Gibco) DMSO or 25µM PARGi inhibitor (PDD00017273, Sigma) for 4 days RNA was extracted using Direct-zol RNA MiniPrep Plus kit (Zymo Research) according to the manufacturer's instructions. The quality of the RNA was measured spectrophotometrically using NanoDrop 2000c (Thermo Scientific) and quality was analysed by RNA ScreenTape Assay and 4200 TapeStation System (Agilent). For RNA-seq, libraries were prepared using Illumina's TruSeq Stranded Total RNA library Preparation Kit according to Illumina's instructions
ENA first public
2026-01-01
INSDC center name
Sir William Dunn School of Pathology, University of Oxford
INSDC status
public
Submitter Id
E-MTAB-16012:U2OS_CC_PARGi_R3
broker name
ArrayExpress
cell line
U2OS
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-16012:U2OS_CC_PARGi_R3
scientific_name
Homo sapiens