BDT5 NIOZ342.9057.9058
plastisphere metagenome
Nanoplastic impacted plastisphere communities
ENA first public
2026-01-01
INSDC center name
NIOZ
INSDC status
public
Submitter Id
NIOZ342.9057.9058
broad-scale environmental context
environment determined by a biofilm on a saline surface [ENVO_01001056]| sea water environment [ENVO_01000321]
collection date
2022-06-10T13:15:00+01
environment package
water|biofilm|sediment
environmental medium
coastal sea water [ENVO_00002150]
experimental factor
Nanoplastics_Incubation
geographic location (country and/or sea)
Netherlands
geographic location (latitude)
+53.002
geographic location (longitude)
+4.79
geographic location (region and locality)
Netherlands:Wadden Sea
local environmental context
biodegradation of plastic [ENVO_06105015]
longhurst zone code
Northeast Atlantic Shelves (NECS)
multiplex identifiers
TAGGTAACCGATTAATCGGATTCC
nucleic acid extraction
https://www.qiagen.com/fi/~/media/5ADF5C38ED8749E1BA4C4966A215A83B.ashx
pcr conditions
initial denaturation:98degC_30sec;denaturation:98degC_10sec;annealing:50degC_15sec;elongation:72degC_30sec;final elongation:72degC_5 min;30 cycles
pcr primers
F: 515F (GTGYCAGCMGCCGCGGTAA) R: 926RBC (CCGYCAATTYMTTTRAGTTT)
project name
BDT5_NP1: Biodegradation Experiment with Nanoplastic Exposure
relationship to oxygen
aerobe
salinity
30.8
sample collection device
PHBH fragment
sample material processing
samples stored in DNA lysis buffer
sample name
NIOZ342.9057.9058
scientific_name
plastisphere metagenome
target gene
16S rRNA| 18S
target subfragment
V4-V5
temperature
20