MSP2 KO 1
Plasmodium falciparum 3D7
Protocols: Cultures were tightly synchronised and harvested at late schizont. Cultures were pelleted, resuspended in TriZol (Invitrogen) and incubated for 5 minutes at 37°C before either immediate RNA extraction or freezing at -80°C. Chloroform (Sigma Aldrich) was added, mixed and then spun at 12,000g for 30 minutes at 4°C. Supernatant was collected and mixed with an equal volume of 70% Ethanol and processed using the RNeasy Kit (Qiagen) to extract purified RNA. RNA was DNAse l (Qiagen) treated for 30 mins at room temperature and cleaned up on RNeasy columns according to the manufacturers protocol. Absence of gDNA was checked by qPCR of gDNA and DNAse I treatment repeated if necessary. Library construction and sequencing was performed by Victorian Clinical Genetics Services (VCGS) Australia. Library was prepared using llumina stranded total RNA kit with Ribo -Zero Plus. QC was performed after libary prepration and prior to sequencing.
ENA first public
2026-01-01
INSDC center name
Malaria Cell Biology, Molecular Biology and Immunology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany Research Centre for Infectious Diseases, School of Biological Sciences, The University of Adelaide, Adelaide, Australia
INSDC status
public
Submitter Id
E-MTAB-15427:MSP2 KO 1
broker name
ArrayExpress
collection date
not collected
common name
malaria parasite P. falciparum
developmental stage
Late Schizont
genotype
MSP2 knockout
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-15427:MSP2 KO 1
scientific_name
Plasmodium falciparum
isolate
3D7