7
Mus musculus
Protocols: Defeated and control animals underwent a final defeat session and were euthanized by decapitation 7 hours after sensory interaction to preserve brain tissue integrity. Hippocampi were dissected and snap-frozen in liquid nitrogen Total RNA was extracted from hippocampal extract with TRIzol Reagent (Invitrogen) following manufacturer protocol and resuspended in DEPC water. RNA concentration and quality was assessed by 4200 Tape Station (Agilent).  Universal Plus mRNA-Seq kit (Tecan Genomics, Redwood City, CA) has been used for library preparation following the manufacturer's instructions (library type: fr-secondstrand). RNA samples were quantified and quality tested by Agilent 2100 Bioanalyzer RNA assay (Agilent technologies, Santa Clara, CA) or Caliper (PerkinElmer, Waltham, MA). Final libraries were checked with both Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and Agilent Bioanalyzer DNA assay or Caliper (PerkinElmer, Waltham, MA).
ENA first public
2026-01-01
INSDC center name
University of Milan, Department of Medical Biotechnology and Translational Medicine
INSDC status
public
Submitter Id
E-MTAB-15221:7
age
90
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
hippocampus
rna sequencing batch
A
sample name
E-MTAB-15221:7
scientific_name
Mus musculus
sex
male
stimulus
CSDS, susceptible response
strain
C57BL/6N