PE-06
Homo sapiens
Protocols: Endometriotic tissues as well as samples of healthy endometrium were harvested laparoscopically at the time of the operation and immediately flash frozen in liquid nitrogen and stored in deep freeze until processing.  Frozen tissues were grinded with mortar and pestle and the total RNA was isolated with Trizol reagent following manufacturer's protocol. 1µg of DNase I-treated RNA isolated from tissues was used for poly-A mRNA isolation with the NEBNext Poly(A) mRNA Magnetic Isolation Module. Paired-end libraries were prepared using the NEBNext Ultra II Directional RNA Library Kit with NEBNext multiplex index oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs).
ENA first public
2026-01-01
INSDC center name
Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV); First Faculty of Medicine; Charles University, Czech Republic
INSDC status
public
Submitter Id
E-MTAB-15117:PE-06
age
41
broker name
ArrayExpress
collection date
not collected
common name
human
developmental stage
adult
disease
Peritoneal endometriosis (PE)
geographic location (country and/or sea)
not collected
individual
6
isolate
not applicable
organism part
peritoneal cavity
sample name
E-MTAB-15117:PE-06
scientific_name
Homo sapiens
sex
female