FGHI4
Homo sapiens
Protocols: THP-1 cells were differentiated using PMA (50 ng/mL) for 3 days, then infected with either WT or mutant Lp 130b strains for 18h at MOI 10.  RNA was extracted using the IsolateII RNA Mini kit (Bioline) according to manufacturer's instruction. After QC using Bioanalyzer, only samples with RIN >8.0 were used. The cDNA libraries for RNA sequencing were generated from 20 ng of RNA using an in-house multiplex RNA-seq method, where sample-specific indices were incorporated during oligo-d(T) priming of first strand cDNA (MHTP Medical Genomics Facility, https://doi.org/10.1038/s41467-021-23111-1).
ENA first public
2026-02-28
INSDC center name
Hudson Institute of Medical Research
INSDC status
public
Submitter Id
E-MTAB-16655:FGHI4
broker name
ArrayExpress
cell line
THP-1
cell type
macrophage
collection date
not collected
common name
human
disease
Legionnaires' disease
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blood
sample name
E-MTAB-16655:FGHI4
scientific_name
Homo sapiens
stimulus
Delta-FGHI Lp130b infection, repeat 3