ont-Crg-CapTrap-Brain-MM
Mus musculus
Protocols: commercial samples obtained from Ambion: Ambion is a brand of life science research products and technologies, mainly focused on RNA products. It is owned by Thermo Fisher Scientific, a company that provides a variety of scientific research products and services. Therefore, \"total RNA commercial samples obtained from Ambion\" refers to RNA samples purchased from this brand for research purposes. CapTrap cDNA library preparation: CapTrap cDNA library preparation is a long-read sequencing protocol that selectively enriches 5'-capped transcripts by capturing the 5' caps of transcripts. This protocol uses Poly(A) tail selection to specifically target mRNA, and a 5' cap-specific oxidation reaction coupled to biotin-streptavidin to capture the 5' cap structure. The captured mRNA is then converted to cDNA and the library is prepared with 5' and 3' specific linkers to enrich for full-length libraries. CapTrap is particularly useful for studying transcription start sites, alternative splicing, and post-transcriptional modifications.
ENA first public
2025-11-20
INSDC center name
Centre for Genomic Regulation (CRG)
INSDC status
public
Submitter Id
E-MTAB-13063_2:ont-Crg-CapTrap-Brain-MM
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
brain
sample name
E-MTAB-13063_2:ont-Crg-CapTrap-Brain-MM
scientific_name
Mus musculus
sex
mixed