Sample 13
Mus musculus
Protocols: In vivo studies conducted in accordance with the German Animal Welfare Act. Generation of Prmt5-cKO mice by mating Prmt5^loxP/loxP mice with alpha-MHC-MerCreMer transgenic mice, followed by Cre recombinase activation via Tamoxifen. Prmt5 genetic deletion induced at 8 weeks with Tamoxifen (75mg/kg BW/d) via oral gavage over two cycles. Post-Tamoxifen, a 3-week recovery period was kept before starting Isoprenaline treatment (30mg/kg BW/d) for 3 days followed by organ harvest. CM nuclear samples: RNA extraction from PCM1-positive nuclei (isolated via fluorescence-activated nuclei sorting) using RNeasy Micro Kit (Qiagen) following manufacturer's protocols. CM nuclear samples: library construction was performed according to the SmartSeq2 protocol (Picelli, S., Faridani, O., Björklund, Å. et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc9, 171–181 (2014). https://doi.org/10.1038/nprot.2014.006 andBianca P Hennig, Lars Velten, Ines Racke, Chelsea Szu Tu, Matthias Thoms, Vladimir Rybin, Hüseyin Besir, Kim Remans, Lars M Steinmetz, Large-Scale Low-Cost NGS Library Preparation Using a Robust Tn5 Purification and Tagmentation Protocol, G3 Genes|Genomes|Genetics, Volume 8, Issue 1, 1 January 2018, Pages 79–89, https://doi.org/10.1534/g3.117.300257)
ENA first public
2026-05-16
External Id
SAMEA114785880
INSDC center name
Klaus Tschira Institute for Integrative Computational Cardiology, Heidelberg, Germany and DZHK (German Center for Cardiovascular Research), Partner Site Heidelberg/Mannheim, Germany
INSDC last update
2023-12-03T10:09:04Z
INSDC status
public
Submitter Id
E-MTAB-13594:Sample 13
age
13
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Cre+ Prmt5 wildtype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
heart left ventricle
sample name
E-MTAB-13594:Sample 13
scientific_name
Mus musculus
sex
male
strain
C57BL/6N