Fol_16
Gallus gallus
Protocols: At Day 28 of the feeding trial, 10 birds of each dietary group were mechanically stunned and slaughtered by decapitation. Tissue samples were collected from the right kidney, pancreas taken in anatomical proximity to the duodenal loop, uterus mucosa collected 2-5 cm upstream of the cloaca, and 5-10 follicles with an average diameter of 3 to 5 mm (large-white follicles). After dissection, the samples were rinsed with saline solution, snap frozen in liquid nitrogen and stored at -80 °C until use. RNA was extracted using TRIzol Reagent (Invitrogen, Karlsruhe, Germany), followed by DNase I digestion (Roche, Mannheim, Germany) and finally clean-up using the RNeasy Mini Spin kit (Qiagen). Total RNA of 2 µg was used for library preparation with the TruSeq Stranded mRNA Kit according to the manufacturer's recommendation (Illumina, San Diego, CA, USA).
ENA first public
2026-02-28
INSDC center name
Research Institute for Farm Animal Biology (FBN) Physiological Genomics Unit
INSDC status
public
Submitter Id
E-MTAB-14922:Fol_16
age
72
broker name
ArrayExpress
collection date
not collected
common name
chicken
developmental stage
adult
diet
Biostrong 510 supplemental diet (150 mg/kg feed)
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
mature ovarian follicle
sample name
E-MTAB-14922:Fol_16
scientific_name
Gallus gallus
sex
female
strain
Lohmann Brown