VCaP_PRT2527_rep2
Homo sapiens
Protocols: Cells were plated in 6-well tissue culture plates at 50% confluence. After 24 h, cells were treated with DMSO or 100 nM PRT2527 for 24 h. Cells were lysed with 500 µl TRIzol Reagent (15596026, Thermo Fisher Scientific) RNA was extracted using Direct-zol RNA MiniPrep kit (R2052, Zymo Research). NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.
ENA first public
2025-05-23
External Id
SAMEA113542839
INSDC center name
Institute of Oncology research (IOR)
INSDC last update
2023-05-23T15:23:31Z
INSDC status
public
Submitter Id
E-MTAB-12991:VCaP_PRT2527_rep2
broker name
ArrayExpress
cell_line
VCaP
cell_type
prostate gland cancer cell
common name
human
disease
prostate cancer
genotype
wild type genotype
organism part
prostate gland
sample name
E-MTAB-12991:VCaP_PRT2527_rep2
scientific_name
Homo sapiens