M168_IR_2
Homo sapiens
Protocols: Cell monolayers were trypsinized, neutralized with medium, washed with PBS, and centrifuged. Washed cell pellets were resuspended in Macherey-Nagel Nucleospin RNA Plus Lysis Buffer. Not applicable- no dissections. DMEM + glutamine + pyruvate + pen/strep in 5% O2 + 5% CO2 incubator.  Proliferating cells were treated with 10 pg/ml Interleukin-alpha (Sigma I2778)for 24 hours. M168 cells were induced into senescence by irradiating with 40 Gy of 120 KV X-rays produced by a Faxitron 43855D generator. Cells were incubated for 10 days post irradiation before harvesting for RNA isolation. For IL1-alpha treatment of irradiated cells, the cells were irradiated with 40 Gy of 120 KV X-rays as above and cells were incubated for 9 days and then treated for 24 hours with 10 pg/ml of IL1-alpha (Sigma I2778). Data were normalized with EdgeR and Limma voom  RNA was extracted with the Macherey-Nagel Nucleospin RNA Plus. NEBNext Ultra II Directional RNA Library Kit with PolyA RNA selection
ENA first public
2026-01-01
INSDC center name
I2BC_CEA/CNRS/Université Paris-Saclay
INSDC status
public
Submitter Id
E-MTAB-16030:M168_IR_2
broker name
ArrayExpress
cell line
M168
cell type
fibroblast
collection date
not collected
common name
human
developmental stage
adult
genotype
Female
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Breast skin
sample name
E-MTAB-16030:M168_IR_2
scientific_name
Homo sapiens
stimulus
40Gy_120kV_X-ray+10days