La Saint-George T1 H
uncultured eukaryote
La Saint-George bulk soil sampled with an auger and a showel at depth 0 to 10 cm and 2mm sieved on site, then soil is gamma sterilized and placed in 200 µm mesh bags, inoculated for 2 months in soil from same plot, in a block of soil of 20x20x15cm (L x l x d) located in the same plot
ENA first public
2026-01-01
INSDC center name
University of Neuchatel
INSDC status
public
Submitter Id
SG-2-H-T1
broad-scale environmental context
temperate mixed broadleaf forest [ENVO:01000389]
collection date
2024-01-30
depth
0-0.1
elevation
1134
environmental medium
soil [ENVO:00001998]|plant litter [ENVO:01000628]
experimental factor
SG T1 H
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.53681
geographic location (longitude)
6.26771
geographic location (region and locality)
Gimel
local environmental context
soil surface layer [ENVO:04000010]|litter layer [ENVO:01000338]
mean annual precipitation
1690.7
mean annual temperature
5.9
multiplex identifiers
bc1019:ACACACTCTATCAGAT; bc1077:TATCTCTCGAGTCGCG
nucleic acid extraction
DNAeasy PowerSoil Pro Kit, QIAGEN
pH
6.27
pH method
five grams of dry soil mixed in 12.5 ml of distilled water
pcr primers
616sfm1:TTAAAAMGCTCGTAGTYG; 616sfm2:TTAAAWCARTTATWRTYT; 28s3rm:ATTAAACWGTYRGATTCCC
project name
Fishing Active Soil Protists
sample name
SG-2-H-T1
sample volume or weight for DNA extraction
0.3
scientific_name
uncultured eukaryote
sieving
2 mm
slope aspect
54
slope gradient
5
target gene
18S; ITS; 28S
total organic carbon
26.9984722137451
total organic carbon method
Thermo ScientificTM FLASH 2000 CHNS elemental analyzer