Bois Chamblard T2 B
uncultured eukaryote
Bois Chamblard bulk soil sampled with auger at four different spots on a 20x20 cm area and 12 cm depth, from a block of soil of 20x20x15cm (L x l x d) maintained at same location for 9 months, sieved at 2mm
ENA first public
2026-01-01
INSDC center name
University of Neuchatel
INSDC status
public
Submitter Id
BC-3-B-T2
broad-scale environmental context
temperate deciduous broadleaf forest [ENVO:01000385]
collection date
2024-08-28
depth
0-0.1
elevation
392
environmental medium
soil [ENVO:00001998]|plant litter [ENVO:01000628]
experimental factor
BC T2 B
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.46601
geographic location (longitude)
6.41102
geographic location (region and locality)
Buchillon
local environmental context
soil surface layer [ENVO:04000010]|litter layer [ENVO:01000338]
mean annual precipitation
1035
mean annual temperature
10.4
multiplex identifiers
bc1011:CTATACGTATATCTAT; bc1057:GACGAGCGTCTGAGAG
nucleic acid extraction
DNAeasy PowerSoil Pro Kit, QIAGEN
pH
5.16
pH method
five grams of dry soil mixed in 12.5 ml of distilled water
pcr primers
616sfm1:TTAAAAMGCTCGTAGTYG; 616sfm2:TTAAAWCARTTATWRTYT; 28s3rm:ATTAAACWGTYRGATTCCC
project name
Fishing Active Soil Protists
sample name
BC-3-B-T2
sample volume or weight for DNA extraction
0.3
scientific_name
uncultured eukaryote
sieving
2 mm
slope aspect
60
slope gradient
1
target gene
18S; ITS; 28S
total organic carbon
5.58074712753296
total organic carbon method
Thermo ScientificTM FLASH 2000 CHNS elemental analyzer