S5
Mus musculus
Protocols: Mouse kidney cortices were isolated, stored in liquid nitrogen, and processed for RNA extraction. Tissue samples were collected with care to preserve RNA integrity, ensuring high-quality downstream analysis. RNA was extracted from mouse kidney cortices using the RNeasy Kit (Qiagen), following the manufacturer's standard protocol. This method includes steps for cell lysis, removal of contaminants, and RNA elution to obtain high-purity RNA for sequencing. Poly-A RNA was enriched from total RNA to select for mRNA transcripts. Library preparation, including cDNA synthesis and adapter ligation, was performed by the McMaster Genomics Facility using protocols optimized for the Illumina NextSeq P2 sequencing platform.
ENA first public
2026-01-01
INSDC center name
McMaster University
INSDC status
public
Submitter Id
E-MTAB-14742:S5
age
8
broker name
ArrayExpress
collection date
not collected
common name
house mouse
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
cortex of kidney
sample name
E-MTAB-14742:S5
scientific_name
Mus musculus
sex
male
strain
C57BL/6