A530016L24Rik-KO_rep2
Mus musculus
Protocols: Embryoid bodies at day 7 (D7) of differentiation were washed three times with PBS by spinning at 1000 rpm for 5 minutes, and dissociated using TrypLE (Gibco, 12604013) in a 37°C water bath while shaking with intermitting pipetting to facilitate dissociation. After 5 minutes, Trypsin/EDTA was added and dissociation continued for another 1 minute while pipetting. The dissociation was inactivated with PBS supplemented with 10% FBS (PBS-FBS), strained with a 40 µM cell strainer, washed three times with PBS-FBS by centrifuging at 300 rpm for 10 minutes and prepared for downstream applications.  Viable single cells (18000 per run, with an estimated recovery of 10000 cells) were encapsulated for cDNA synthesis and barcoded on a Chromium X using the Chromium Single Cell G Chip and final libraries were generated using the 10X Chromium Next GEM Single Cell 3' kit v3.1 (10X Genomics) and instructions.  Final libraries were generated using the 10X Chromium Next GEM Single Cell 3' kit v3.1 (10X Genomics) and instructions. Quality and quantity of the sequence libraries were determined with a High Sensitivity DNA kit using an Agilent 2100 Bioanalyzer (Agilent).
ENA first public
2026-01-15
INSDC center name
Erasmus University Medical Center
INSDC status
public
Submitter Id
E-MTAB-15618:A530016L24Rik-KO_rep2
broker name
ArrayExpress
cell line
ESC derived cell line
cell type
Embryoid body at day 7
collection date
not collected
common name
house mouse
developmental stage
Embryoid body at day 7
genotype
A530016L24Rik knockout
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-15618:A530016L24Rik-KO_rep2
scientific_name
Mus musculus
strain
129X1/SvJ