Dhx36 cKO
Mus musculus
Protocols: Whole hearts from 7-day-old PD7 wild-type (WT) (Dhx36f/f;Tnnt2 wt/wt) and mutant (Dhx36f/f;Tnnt2 cre/wt) mice were dissected, chopped into several pieces, snap-frozen and stored in liquid nitrogen. On the day of the experiment, wild-type and mutant hearts were pooled (two each), and nuclei were isolated using the Chromium Nuclei Isolation Kit with RNAse inhibitor (10X Genomics; #PN-1000494). Nuclei were captured in the Chromium Controller (10X Genomics) and processed with the Chromium Next GEM single cell Multiome ATAC+Gene Expression (GEX) Kit (10X Genomics; #PN-1000285) ATAC and GEX libraries from the same pool of pre-amplified Tn5 transposed DNA/cDNA
ENA first public
2026-01-01
INSDC center name
Institute for Rare Diseases Research, Instituto de Salud Carlos III (ISCIII).
INSDC status
public
Submitter Id
E-MTAB-14442:Dhx36 cKO
age
7
broker name
ArrayExpress
cell_type
heart cells
collection date
not collected
common name
house mouse
developmental stage
PD7
genotype
Dhx36 cardiomyocyte conditional KO
geographic location (country and/or sea)
not collected
individual
2 hearts
isolate
not applicable
organism part
heart
sample name
E-MTAB-14442:Dhx36 cKO
scientific_name
Mus musculus
sex
mix
strain
C57bl/6-CD1