Sample 6
Homo sapiens
Protocols: HPAEpiCs were cultured at the ALI for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. HPAEpiCs on ALI day 0, 7, 14, 21, and 28 were washed with PBS and lysed with Lysis buffer in Qiagen RNeasy Mini Kit (Qiagen). Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit (Qiagen). A DNA library was prepared using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc.).
ENA first public
2026-01-01
INSDC center name
Japan Tobacco Inc.
INSDC status
public
Submitter Id
E-MTAB-14793:Sample 6
age
20
broker name
ArrayExpress
cell_type
epithelial cell
collection date
not collected
common name
human
developmental stage
embryo stage
disease
normal
geographic location (country and/or sea)
not collected
growth condition
ALI culture
individual
Donor 1
isolate
not applicable
organism part
alveolus of lung
sample name
E-MTAB-14793:Sample 6
scientific_name
Homo sapiens
sex
female