Saint-Livres T2 H
uncultured eukaryote
Saint-Livres bulk soil sampled with an auger and a showel at depth 0 to 10 cm and 2mm sieved on site, then soil is gamma sterilized and placed in 200 µm mesh bags, inoculated for 9 months in soil from same plot, in a block of soil of 20x20x15cm (L x l x d) located in the same plot
ENA first public
2026-01-01
INSDC center name
University of Neuchatel
INSDC status
public
Submitter Id
SL-3-H-T2
broad-scale environmental context
temperate deciduous broadleaf forest [ENVO:01000385]
collection date
2024-08-28
depth
0-0.1
elevation
670
environmental medium
soil [ENVO:00001998]|plant litter [ENVO:01000628]
experimental factor
SL T2 H
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.52275
geographic location (longitude)
6.38431
geographic location (region and locality)
Saint-Livres
local environmental context
soil surface layer [ENVO:04000010]|litter layer [ENVO:01000338]
mean annual precipitation
1181.6
mean annual temperature
9.1
multiplex identifiers
bc1016:CATAGAGAGATAGTAT; bc1062:TCTCAGATAGTCTATA
nucleic acid extraction
DNAeasy PowerSoil Pro Kit, QIAGEN
pH
4.83
pH method
five grams of dry soil mixed in 12.5 ml of distilled water
pcr primers
616sfm1:TTAAAAMGCTCGTAGTYG; 616sfm2:TTAAAWCARTTATWRTYT; 28s3rm:ATTAAACWGTYRGATTCCC
project name
Fishing Active Soil Protists
sample name
SL-3-H-T2
sample volume or weight for DNA extraction
0.3
scientific_name
uncultured eukaryote
sieving
2 mm
slope aspect
341
slope gradient
4
target gene
18S; ITS; 28S
total organic carbon
3.06668066978455
total organic carbon method
Thermo ScientificTM FLASH 2000 CHNS elemental analyzer