794
Mus musculus
Protocols: Tissue samples were removed, weighed, snapped frozen in liquid nitrogen, and stored at -70°C. Total RNA was isolated using TRI reagent (Sigma-Aldrich, Taufkirchen, Germany). DNA traces were removed by DNaseI-treatment (Baseline-Zero DNase; Biozym, Hessisch Oldendorf, Germany), and the RNA was cleaned-up using the RNA Clean&Concentrator-25 Kit (Zymo Research, Freiburg, Germany). RNA quantity was determined using a Qubit fluorometer (ThermoFisher Scientific, Germany), and integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). 1 µg of total RNA with RIN > 8 was used for library preparation using a TruSeq Stranded mRNA Kit with 11 cycles of PCR-amplification according to the manufacture's recommendation (Illumina).
ENA first public
2025-11-20
External Id
SAMEA110586979
INSDC center name
Research Institute for Farm Animal Biology (FBN) Institute of Genome Biology
INSDC last update
2022-08-09T15:43:49Z
INSDC status
public
Submitter Id
E-MTAB-12065:794
age
70
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
disease
normal
genotype
wild type genotype
isolate
not applicable
organism part
subcutaneous adipose tissue
sample name
E-MTAB-12065:794
scientific_name
Mus musculus
sex
male
strain
DUhTP