Homo sapiens
Protocols: MCF-10-2A TetOn-CENPA-FLAG-HA cells stably expressing a dominant-negative p53 vector (p53-DN) were cultured for 24 days in the absence (no Dox) or presence of Dox (10 ng/mL) to induce basal or high CENP-A expression, respectively. Culture medium (+/- Dox) was renewed every 2-3 days. We harvested cells at days 10 and 24 post-induction and prepared 4 million cells/mL in ice-cold cryopreservation medium (50% fetal bovine serum, 40% growth medium and 10% dimethyl sulfoxide). Single-nuclei suspensions prepared using 10X Nuclei Isolation protocols. Library construction by Active Motif services using the 10x Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent kit. Steps included nuclei transposition, generation of Gel Beads in Emulsion (GEMs) and 10X barcoding, cleanup, pre-amplification of ATAC DNA and cDNA, amplification with P5 and P7 adapters, Illumina sequencing.
ENA first public
2026-04-21
INSDC center name
Institut Curie, Université PSL, Sorbonne Université, CNRS, UMR 3664 Nuclear Dynamics, Chromatin Dynamics lab
INSDC status
public
Submitter Id
E-MTAB-15582:MCF_1DOX_D24_ATAC
broker name
ArrayExpress
cell line
MCF10-2A
cell type
Breast cell
collection date
not collected
common name
human
developmental stage
Adult
disease
Breast fibrocystic disease
genotype
p53-DN TetOn-CENPA-FLAG-HA
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Mammary gland
sample name
E-MTAB-15582:MCF_1DOX_D24_ATAC
scientific_name
Homo sapiens
sex
female