Ovis aries
Protocols: Tissues were dissected as described in (https://www.biorxiv.org/cgi/content/short/2025.08.21.671502v1) and snap-frozen in liquid nitrogen. The frozen samples were lysed in RNA lysis buffer (Qiagen) and refined to a fine suspension using a hand-held motorised homogeniser with a 5 mm probe (Pro-Scientific). RNA extraction was performed using the RNA Clean & Concentrator-5 kit (Zymo). The integrity of RNA was assessed by Agilent Bioanalyzer. Strand-specific RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (Roche), which depletes ribosomal RNA prior to library construction. Libraries were quality-checked and quantified before pooling.
ENA first public
2026-07-08
INSDC center name
Max Delbrück Center for Molecular Medicine
INSDC status
public
Submitter Id
E-MTAB-17285:SCG_fetal_d145_6a
broker name
ArrayExpress
collection date
not collected
common name
sheep
developmental stage
fetal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
gestational age
145
individual
bl5571
isolate
not applicable
organism part
superior cervical ganglion
sample name
E-MTAB-17285:SCG_fetal_d145_6a
scientific_name
Ovis aries
sex
female
strain
Welsh mountain