soil metagenome
Seasonal wetland soil sample from rainy season
collection_date
2023-04-21
depth
10 - 20 cm
elev
717
env_broad_scale
ENVO:01001220
env_local_scale
ENVO:00000389
env_medium
ENVO:00001998
geo_loc_name
Brazil: Sao Paulo
lat_lon
22.48889 S 47.48753 W
annual_precpt
1,366.8 mm
annual_temp
21.6 °C
collection_method
Soil cores collected using a closed wetland auger. Samples stored on ice and transported to the laboratory for DNA extraction using the DNeasy PowerSoil Kit (Qiagen)
ph
5,65 ± 0,01
ph_meth
pH measured in water, in duplicate, using a calibrated digital pH meter (soil:water ratio 1:2.5)
pool_dna_extracts
no
samp_collect_device
wetland soil auger
samp_vol_we_dna_ext
0.2397
season_precpt
183.9 mm
season_temp
>22 ºC
tot_nitro
3192 ± 20
tot_nitro_cont_meth
Kjeldahl digestion method, with quantification via titration. Results in mg Kg-1. Duplicate.
tot_org_c_meth
wet oxidation using a strongly acidic dichromate solution. Results in %. Duplicate.
tot_org_carb
3.7 ± 0.28
water_content
31.51 ± 0.36
water_content_soil_meth
Gravimetric method:105 °C for 24 hours in a drying oven. Results in %. Duplicate.
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.soil