Mus musculus
Protocols: Mice were euthanized by cervical dislocation, and eyes were harvested for obtaining RPE/eyecup separated from neural retina. In 1x phosphate buffer saline (PBS, pH 7.4), the anterior segment of the eye was removed, followed by the separation of the neural retina from the eyecup. RPE/Eyecups were stored in a −80 °C freezer until further processing. DNA was extracted from mice, one RPE/eyecup per sample, using the Dneasy Blood & Tissue Kit (Qiagen, Hilden, Germany, Cat# 69506). DNA was eluted in 50 μL AE Buffer. The concentration was measured with a Qubit instrument (Life Technologies, Carlsbad, CA, USA), and 100 ng of genomic DNA was treated with RNAse enzyme at 37 °C for 30 min to remove contaminating RNA. RRBS libraries were prepared by a Zymo-Seq RRBS Library kit (Zymo research, Irvine, CA, USA, Cat# D5460). The libraries were subjected to size selection using magnetic AMPure XP beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA) to enrich DNA fragments of the desired size. The quality control of the final libraries (size-selected and barcoded) was performed using an Agilent TapStation 4200 (D1000, D5000; Agilent Technologies, Santa Clara, CA, USA).
ENA first public
2025-11-04
INSDC center name
UCLA Jules Stein Eye Institute and Department of Ophthalmology, David Geffen School of Medicine
INSDC status
public
Submitter Id
E-MTAB-16049:1-mo PKE2
age
1
broker name
ArrayExpress
collection date
not collected
common name
house mouse
genotype
Abca4-/-
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
RPE/eyecup
sample name
E-MTAB-16049:1-mo PKE2
scientific_name
Mus musculus
strain
129/Sv