Staphylococcus aureus
Protocols: S. aureus isolates from chronic rhinosinusitis patients (CSS) and healthy individuals (MIN) were cultured overnight.Cultures were diluted in TSB broth to an optical density (OD600) of 0.05 the following day, and samples were collected at 3- and 6-hours post-dilution.  RNA from harvested samples were isolated using Zymo Research Quick-RNA Fungal/Bacterial Miniprep Kit standard protocol and rRNA was depleted using Invitrogen MICROBExpress™ Bacterial mRNA Enrichment Kit kit. Nucleic acid library was prepared using standard Illumina RNA-seq protocol that uses random hexamer primers that for cDNA synthesis.
ENA first public
2026-02-12
INSDC center name
Institute for Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany
INSDC status
public
Submitter Id
E-MTAB-16578:RNA6hCSS126_libLAC3055
broker name
ArrayExpress
clinical information
chronic rhinosinusitis patients (CSS)
collection date
not collected
disease
chronic rhinosinusitis
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-16578:RNA6hCSS126_libLAC3055
scientific_name
Staphylococcus aureus
strain
CSS126