Homo sapiens
Protocols: Cells were automatically samples using the ChronoSeq device. 10μl of Time-Tagged beads at 450 beads/μl suspended in lysis buffer were directly mixed with human cells at 600cells/μl. In order 1->12 K562 grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C.  https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. Beads are washed once with 6X SSC buffer and then twice with 1.25X RT buffer. Beads were then pooled together for a single RT reaction. very similar to Drop-seq. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html
ENA first public
2025-11-04
INSDC center name
UCSD
INSDC status
public
Submitter Id
E-MTAB-15966:N706_Bulk_K562_Control_600_Cells
broker name
ArrayExpress
cell concentration
600
cell line
K562
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
sample name
E-MTAB-15966:N706_Bulk_K562_Control_600_Cells
sampling interval
10
scientific_name
Homo sapiens
time-tag injection order
1->12