Mus musculus
Protocols: Seven days after cardiotoxin-induced muscle injury, Tibialis Anterior (TA) muscles from Tg:Pax7-nGFP mice exposed to normoxic (20%) or hypoxic (10%) environment were dissected, minced and incubated with a mix of 2.4U/ml Dispase II (Roche, #04942078001) and 100μg/ml collagenase A (Roche, #11088793001) in 4.5g/L glucose 1x DMEM (GibcoTM; #11995) at 37 °C on a shaking plate for 2 h. The muscle suspension was filtrated through 100-μm and 70-μm cell strainers and then spun at 50g for 10min at 4°C to remove large tissue fragments. Then, the supernatant was collected, filtered through a 40-μm cell strainer and washed by centrifugation at 700g for 10 min at 4°C. The final pellet has been resuspended in cold 4.5g/L glucose 1xDMEM supplemented with 0.2% bovine serum albumin (BSA, Sigma-Aldrich). The muscle cell suspension was used to sort 140 000 GFP+ MuSCs by fluorescence-activated cell sorting (FACS)-sorting using the GFP reporter (n=4 per atmospheric condition). Total RNA was extracted from GFP+ myogenic cells isolated from injured TA using RNAqueous™-Micro Kit RNA Isolation ™ (Invitrogen™, #AM1931). Library preparation and sequencing was performed by the Biomics platform at the Institut Pasteur. RNA preparation was used to build libraries using an Illumina Stranded mRNA library Preparation Kit (Illumina, USA) following the manufacturer's protocol. Quality control was performed on an Agilent BioAnalyzer
ENA first public
2025-11-21
INSDC center name
1. Institut Pasteur, Université Paris Cité, Plate-forme Technologique Biomics, F-75015 Paris, France 2. Institut Pasteur, Université Paris Cité, Bioinformatics and Biostatistics Hub, F-75015 Paris, France
INSDC status
public
Submitter Id
E-MTAB-16208:Norm4
broker name
ArrayExpress
cell line
stem cell derived cell line
cell type
stem cell
collection date
not collected
common name
house mouse
geographic location (country and/or sea)
not collected
growth condition
normoxia
injury
acute muscle injury by cardiotoxin intramuscular injection
isolate
not applicable
organism part
skeletal muscle
sample name
E-MTAB-16208:Norm4
scientific_name
Mus musculus