Mus musculus
strain
mus mus
isolate
H4SV cells
age
cell culture passage 3-4
collection_date
2023-12-12
geo_loc_name
not applicable
sex
female
tissue
fibroblast cell
cell_line
H4SV cells
cell_type
fiberblast cell
collected_by
Song Lou
culture_collection
not applicable
genotype
WT
growth_protocol
DMEM supplemented with 10% FBS and antibiotics in humidified atmosphere with 5% CO2 at 37
isolation_source
H4SV cells were crosslinked with AMT . The cross-linked RNA was extracted and fragmented using ShortCut RNase III. The purified and concentrated RNA fragments were proximity ligated by T4 RNA ligase 1 . Proximity-ligated RNA fragments were irradiated with UV254 for 30 mins. After reverse crosslinking, RNA was purified with three volumes of ethanol and 1 l GlycoBlue. Isolated RNA was reverse transcribed using Superscript II Reverse Transcriptase. Quantitative real-time PCR was performed using desgined primers.
store_cond
the RNA was extracted once the cell tissue is collected
treatment
designed primers for binding sites3, repeat1
BioSampleModel
Model organism or animal