Homo sapiens
Protocols: Epitope-specific CD4 T cells were isolated from PBMC cultured in vitro in presence of epitope peptides (10 ug/mL) for ~12-15 days, with IL2 addition to the medium at days 4 and 8. Isolation was performed using DRB1*03:01 tetramers carrying the epitope peptides and flow cytometry-based cell sorting.  Total RNA was extracted from tetramer-sorted cells using RNeasy kit (Qiagen). Libraries were prepared from 250 ng of RNA with RQN>7.2, using the Illumina TruSeq v2 platform and following manufacturer's instructions.
ENA first public
2025-11-21
INSDC center name
UMass Chan Medical School
INSDC status
public
Submitter Id
E-MTAB-16144:AB_12
broker name
ArrayExpress
cell type
CD4-positive, alpha-beta T cell
collection date
not collected
common name
human
developmental stage
adult
disease
Herpesviridae infectious disease
geographic location (country and/or sea)
not collected
individual
d368
isolate
not applicable
organism part
blood
response to treatment
In vitro expanded 12-15 days
sample name
E-MTAB-16144:AB_12
scientific_name
Homo sapiens
stimulus
HCMV epitope