metagenome
This study investigates the nitrogen removal potential of a synthetic microbial community (SynCom) derived from marine plastic biofilms (the "plastisphere"). Three bacterial strains (Pseudomonas stutzeri P5F4-3, Shimia sp. pe10, and Ruegeria sp. PMMA7) were isolated from this environment and assembled into a defined SynCom. The community was cultured in Marine Broth 2216E with carbon sources supplied at 10% of the standard concentration, under nitrate stress (20 mg/L NO3-N). 16S rRNA gene (V3-V4 region) amplicon sequencing was performed. This BioSample represents the SynCom cultured at 7% salinity for 24 hours (biological replicate 1).
collection_date
2025-03-01
depth
1 m
elev
-1
env_broad_scale
Marine coastal water
env_local_scale
Anthropogenic marine biofilm on plastic surface
env_medium
Marine Broth 2216E
geo_loc_name
China: Qingdao, Shandong
lat_lon
36.07 N 120.38 E
biomass
OD=0.8
chlorophyll
not applicable
collection_method
Plastic sheets deployed in nylon mesh bags; biofilm removed by sonication and vortexing
isolation_source
Biofilm on submerged polyethylene plastic sheet
nitrate
20 mg/L
nitrite
0
oxy_stat_samp
aerobic
perturbation
High-salinity (7%) and nitrate amendment (20 mg/L NO3-N) stress treatment
ph
8.5
phosphate
Disodium Phosphate（磷酸二钠） 0.008g
pressure
1 atm
salinity
7%
samp_collect_device
Nylon mesh bag
samp_mat_process
Cells were harvested by centrifugation and snap-frozen in liquid nitrogen
samp_store_temp
-80 degree Celsius
samp_vol_we_dna_ext
1 g
size_frac
>0.22 um
source_material_id
Dnitrification-syn7%-24h-1
temp
25
turbidity
0.8
water_content
1
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.microbial