Mus musculus
Protocols: Bone marrow was harvested from femur, tibia and hip bones in mice. Total bone marrow was depleted of differentiated hematopoietic cells (lineage-positive cells) using Mouse Hematopoietic Progenitor (Stem) Cell Enrichment Set (BD). Magnetically sorted Lineage-negative (lin-) cells were kept overnight at 4°C in IMDM medium supplemented with 10% FBS (HyClone) and 1% penicillin-streptomycin (Thermofisher). Staining was performed for 20min at room temperature (RT) using CD3ε (Lin) – APC clone 145-2C11 (553066, BD), TER-119 (Lin) – APC clone Ter-119 (557909, BD), CD45R/B220 (Lin) – APC  clone RA3-6B2 (553092, BD), Ly6G-6C (Lin)-APC clone RB6-8C5 (553129, BD), Ly-6A/E (Sca-1) - PeCy7 clone d7 (558162, BD), CD117 (c-Kit) – PE or PerCP-Cy5.5,  clone 2B8 (553355 or 560557 respectively, BD), CD34– FITC or AF700 clone RAM34 (560238 or 560518, BD), CD135 (Flk2) – BV421 or PE clone A2F10.1 (562898 or 553842 respectively, BD). Hematopoietic stem cells (Lin-Sca+c-Kit+CD34lowFlk2-) were sorted using ARIA3, ARIA Fusion or Influx cell sorters (BD Franklin Lakes, NJ, USA) and collected in Stem Span (StemCell).  Tet2-/- C57BL/6J mice (2-4 months-old)  were housed in a specific pathogen-free environment. All procedures were reviewed and approved by the Animal Care Committee (APAFIS #23286-2019121214392327 and #35147-2022020315419972). intraperitoneal injections of PBS or 6ug LPS were performed every two days for 30 days CUT&Tag-IT assay kit (Active Motif) was used on 3,000 HSC according to manufacturer's instructions. Cells were incubated O/N with 0.5µg of H3K9me3 (C15410056-Diagenode). Library construction was performed using the Cut&Tag-IT Assay kit (Active Motif) according to manufacturer's instructions
ENA first public
2026-07-28
INSDC center name
Inserm UMR1287, Université Paris-Saclay, Gustave Roussy, 94805, Villejuif, France.
INSDC status
public
Submitter Id
E-MTAB-15444:Tet2-/- PBS -1
age
2-4
broker name
ArrayExpress
cell type
hematopoietic stem cell
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Tet2-/-
geographic location (country and/or sea)
not collected
immunophenotype
LSK FLK2- CD34-
isolate
not applicable
organism part
bone marrow
sample name
E-MTAB-15444:Tet2-/- PBS -1
scientific_name
Mus musculus
sex
female
strain
C57BL/6J