Homo sapiens
Protocols: Cells harvested into QIAGEN RLT buffer. Pellets homogenized with molecular grinding resin and plastic pestle. hESC/hiPSC cultured in TeSR™-E8™ on vitronectin. Differentiation to iMSCs via MesenPRO RS™. 3D pellets (200k cells) cultured in medium with TGFβ3 and BMP2. RNA extracted using QIAGEN RNeasy mini kit, as per manufacturers instructions. mRNA libraries were prepared using the Illumina stranded mRNA prep kit.
ENA first public
2026-02-12
INSDC center name
University of Manchester,
INSDC status
public
Submitter Id
E-MTAB-16599:SW-147_D21_MUT
broker name
ArrayExpress
cell line
MAN13
cell type
chondrocyte
collection date
not collected
common name
human
developmental stage
Day 21 cartilage pellet
disease
Multiple epiphyseal dysplasia
genotype
MATN3 V194D heterozygous
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Cartilage
sample name
E-MTAB-16599:SW-147_D21_MUT
scientific_name
Homo sapiens