Mus musculus
sample_name
Fucci_ESC_mid_G1_R1
cell_line
R26p-Fucci2 mESC
cell_type
embryonic stem cells
collection_date
missing: lab stock
dev_stage
embryonic
geo_loc_name
missing: lab stock
growth_protocol
Mouse embryonic stem cells were maintained in 2i/LIF medium consisting of NDiff 227 base medium supplemented with the two inhibitors (2i) PD0325901 (1 uM) and CHIR99021 (3 uM), plus leukemia inhibitory factor (LIF; 1000 U/mL) on iMatrix-511-coated dishes. Cells were cultured at 37 C with 5% CO2 and passaged every 2-3 days using trypsin/EDTA.
sex
female
strain
BDF1 (C57BL/6N x DBA/2N)
tissue
embryonic stem cells
treatment
Fixation with 1% formaldehyde followed by FACS sorting