Pimephales promelas
strain
GLTED-Duluth-wt
isolate
not applicable
breed
not applicable
cultivar
not applicable
ecotype
not applicable
age
5-6 dpf
dev_stage
larvae
collection_date
2023-05-09
geo_loc_name
USA: Minnesota, Duluth
sex
not determined
tissue
whole body
biomaterial_provider
US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804
birth_location
US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804
breeding_method
All larval fathead minnows (Pimephales promelas) used for testing were obtained from an on-site culture facility at the US EPA Great Lakes Toxicology and Ecology Division in Duluth, MN, USA. Fathead minnow embryos were collected from multiple crosses of at least three females in paired breeding. Fertilized embryos that were attached to polyvinyl chloride breeding tiles were transferred to a bath of aerated Lake Superior water and held at 25 °C. All embryos were fertilized within a two-hour window. At five-day post-fertilization, larvae that had hatched and were freely swimming were collected, pooled, and randomly sorted for use. All procedures involving larval fathead minnows were approved by an animal care and use committee in accordance with the Guide for the Care and Use of Laboratory Animals (National Research Council, 2011; US EPA Animal Care and Use Plan # Eco24-09-001).
collected_by
Great Lakes Toxicology & Ecology Division
sample_type
Total RNA
store_cond
-80 deg C
measured_conc
0.0108
measured_conc_units
μM
nominal_conc
0.034364
nominal_conc_units
μM
treatment
Fluticasone Propionate (DTXSID8045511)
plate_ID
3
replicate_ID
C
BioSampleModel
Model organism or animal