Caenorhabditis elegans
Protocols: Young adult worms were harvested and washed with M9 buffer and 0.2 % diethyl pyrocarbonate (DEPC) treated water, 3 times each, for removing any adherent bacteria and to inactivate RNase enzymes. QIAzol Lysis Reagent (1000μl) was added and worms were homogenized using pestle. RNA was isolated using QIAzol Lysis Reagent and miRNeasy Mini Kit. 500ng of total RNA was used for the construction of sequencing libraries. The Libraries were made using KAPA RNA HyperPrep Kit Illumina® Platforms following manufacturer's protocol.
ENA first public
2026-04-21
INSDC center name
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India and CSIR-Central Drug Research Institute, Lucknow 226031, India
INSDC status
public
Submitter Id
E-MTAB-15794:N2_1.4
age
3
broker name
ArrayExpress
collection date
not collected
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
growth condition
Escherichia coli OP50 lawn spread over Nematode Growth Media (NGM)
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-15794:N2_1.4
scientific_name
Caenorhabditis elegans
sex
hermaphrodite
strain
N2