Homo sapiens
Protocols: Human bone marrow-mesenchymal stem cells (h-BM-MSCs) were purchased from Lonza (Basel, Switzerland, PT-2501). SSEA-3(+) Muse cells were collected by fluorescence-activated cell sorting (FACS; BD FACSAria™ II cell sorter, Becton Dickinson, San Jose, CA, USA). mCherry- or GFP-h-Muse cells (1 × 105) were incubated for 3 days with m-cardiomyocyte DDCs, r-neural cell DDCs, and r-hepatic cell DDCs derived from 2 × 105 cells to make Phago-cardio-, Phago-neuro-, and Phago-hepa-Muse cells, respectively, then washed out and cultured in medium (DMEM + 10% FBS) for 4 days. Cells for all samples were stained with barcode tag-conjugated β2-microglobulin antibody and CD298 antibody (BioLegend), counted and multiplexed, and prepared for single-cell capture. Single-cell capture and cDNA synthesis were performed by the BD Rhapsody Single-Cell Analysis System according to the manufacturer's instructions.
ENA first public
2026-04-21
INSDC center name
Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine
INSDC status
public
Submitter Id
E-MTAB-16736:Sample1_WTA
broker name
ArrayExpress
cell type
Phago-cardio-, Phago-neuro-, and Phago-hepa-Muse cells
collection date
not collected
common name
human
developmental stage
adult
fraction
Naive, Phagocytosed-cardiomyocyte fragments, Phagocytosed-neuronal cell fragments, Phagocytosed-hepatocyte fragments
geographic location (country and/or sea)
not collected
isolate
not applicable
progenitor cell line
h-BM-MSCs
progenitor cell type
bone marrow-mesenchymal stem cell
sample name
E-MTAB-16736:Sample1_WTA
scientific_name
Homo sapiens