sediment metagenome
Samples were obtained from an actively methanogenic sandy zone of the contaminated aquifer, from a depth of approximately 6 m (Pensacola, Florida).. Aquifer sediments (100 g) were added to sterile, anoxic 250-mL screw cap bottles, topped with 100 mL of sterile, anoxic minimal medium, and amended with 40 �M each of toluene, o-xylene, p-xylene, and ethylbenzene. Upon confirmation that toluene and o-xylene were susceptible to biodegradation, one microcosm was selected to be solely maintained on toluene. This became the parent culture of DGG-T. DGG-T has since been subcultured numerous times and is now maintained in large stainless steel vessels.
ENA first public
2026-07-16
INSDC center name
UofA
INSDC status
public
Submitter Id
DGGT
collection date
1990
geographic location (country and/or sea)
USA
sample name
DGGT
scientific_name
sediment metagenome