soil metagenome
Bulk mesofauna samples were subjected to DNA extraction using the DNeasy Blood and Tissue Kit (Qiagen) on a KingFisher Flex robotic platform (Thermo Scientific). Samples were homogenized prior to extraction, and DNA extracts were quantified using a NanoDrop 1000 spectrophotometer. A 418 bp fragment from the 3' end of the mitochondrial COI barcode region (bc3') was amplified using primers Ill-B-F and Fol-degen-R with Illumina adapter tails. Three independent PCR reactions were performed per sample and pooled. Amplicons were purified using AMPure XP magnetic beads and used as template for a limited-cycle PCR to incorporate dual-index barcodes and Illumina sequencing adapters. Libraries were generated for high-throughput sequencing on Illumina platforms.
isolate
Bulk of mesofauna specimens LP06_DEEP
breed
not applicable
isolation_source
soil mesofauna community samples
collection_date
2016/2020
geo_loc_name
Spain: Canary Islands
tissue
full body
biomaterial_provider
Institute of Natural Products and Agrobiology (IPNA-CSIC)
collected_by
Island Ecology and Evolution Group (GEEI)
env_broad_scale
Macaronesian Laurel Forest
lat_lon
28.630344 N 17.826626 W
BioSampleModel
Metagenome or environmental