Mus musculus
Protocols: After 8 month the animals were killed by cervical dislocation and the hearts were removed, frozen in liquid nitrogen and stored at -80°C for further processing. The heart tissue was ground to a powder with mortar and pestle under liquid nitrogen. We used the 2-hit regimen20, in which mice were fed with a high-fat diet (D12492, Research Diet Inc) and L-NAME 0.5g/L (nitric oxide synthase inhibitor) in drinking water, beginning at 3 months of age and continue for 16 weeks. Control mice were fed with a control diet (D12450K, Research Diet Inc) and water without L-NAME. After 16 weeks on the 2-hit regimen, when the mice had fully developed the HFpEF-like phenotype, 2hit mice and control mice were randomly received Rbm20-ASOs or PBS treatment. Rbm20-ASOs were administered through subcutaneous injections at 25 mg/kg, once a week for 6 weeks. Following this treatment period (6 weeks of ASO/PBS treatment, or 22 weeks in a 2-hit regimen), these mice were employed in endpoint studies RNA from 4 left ventricles per group were isolated using TRIzol (Thermo Fisher), treated with DNAseI (Qiagen) followed by a clean-up with the Qiagen RNA micro kit.  The cDNA libraries were generated using the TruSeq Stranded mRNA Sample Prep Kit (Illumina). Both RNA integrity and cDNA library size distribution were confirmed with TapeStation (Agilent Technologies). Concentration of indexed libraries was determined using Qubit High-Sensitivity DNA kit (Invitrogen).
ENA first public
2026-03-19
INSDC center name
Translational Cardiology and Functional Genomics, Max Delbrück Center for Molecular Medicine in the Helmholtz Association; 13125 Berlin, Germany; German Center for Cardiovascular Research (DZHK), partner site Berlin; 10785 Berlin, Germany
INSDC status
public
Submitter Id
E-MTAB-14882:Ctrl_ASO_3
age
8
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
diet
Ctrl diet
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
Ctrl_ASO_3
isolate
not applicable
organism part
heart left ventricle
sample name
E-MTAB-14882:Ctrl_ASO_3
scientific_name
Mus musculus
sex
male
strain
C57BL/6N