Homo sapiens
Protocols: Human tumor cell line A549 (lung adenocarcinoma), transfected with luciferase and mKate fluorescent protein. Selected samples were co-cultivated with cancer-assosiated fibroblasts (CAFs), obtained from brain metastazis of 2 human patient. CAFs were isolated he indirect magnet-activated cell sorting (MACS) using the fibroblast-specific kit (Fibroblast MicroBeads, Miltenyi, Bergisch Gladbach, Germany) according to the manufacturer´s instructions. Samples were inoculated in DMEM with 10% FBS for 24 hours. After that time, samples were cultured for 48 hours. CAFs were sorted from samples, so only tumor cells were procesed. Sorting purity was greater than 98%, reaching 100%.  Total RNA was isolated by the Qiagen RNeasy Micro Kit (Qiagen), according to the manufacturer's protocol. For the library construction, the KAPA mRNA HyperPrep Kit with Poly(A) mRNA selection (Roche) was used with starting amount of 1000 ng of total RNA.
ENA first public
2026-07-28
INSDC center name
Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Czech Academy of Sciences:
INSDC status
public
Submitter Id
E-MTAB-15451:A1
broker name
ArrayExpress
cell line
A549
cell type
lung cancer cell
collection date
not collected
common name
human
developmental stage
adult
disease
cancer
genotype
wild type genotype
geographic location (country and/or sea)
not collected
growth condition
solo culture
isolate
not applicable
organism part
lung
sample name
E-MTAB-15451:A1
scientific_name
Homo sapiens