Homo sapiens
Protocols: UCL93 and KO cells were plated at 5x10^5 cells/mL in 10 cm2 plates and incubated at 33°C under 70% confluency was reached. Total RNA was extracted from the cells (n=3/ cell line).  Total RNA was extracted from the cells (n=3/ cell line) using a Trizol reagent and stored at -80°C. The RNA concentration and quality were determined using a Thermo Scientific™ NanoDrop™ spectrophotometer and Agilent 2100. After rRNA removal at Novogene, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
ENA first public
2026-07-28
INSDC center name
KRUK Postdoctoral Research Associate
INSDC status
public
Submitter Id
E-MTAB-14624:PC1KO_3
broker name
ArrayExpress
cell_line
UCL93
cell_type
epithelial cell
collection date
not collected
common name
human
genotype
PC1 knock out
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
kidney
sample name
E-MTAB-14624:PC1KO_3
scientific_name
Homo sapiens