soil metagenome
Composite topsoil sample (P3) collected at 0-10 cm depth from the Caatinga Experimental Field (CEF) in Petrolina, Pernambuco, Brazil, during the rainy season. This sample represents preserved Caatinga dry forest and was collected for shotgun metagenomic sequencing of Caatinga tropical dry forest soil microbial communities.
collection_date
2015-05-05
depth
0-10 cm
elev
375 m
env_broad_scale
Caatinga tropical dry forest biome
env_local_scale
preserved Caatinga tropical dry forest area
env_medium
soil
geo_loc_name
Brazil: Pernambuco, Petrolina
lat_lon
9.050261 S 40.318322 W
agrochem_addition
not applicable
altitude
375 m
annual_precpt
below 800 mm/year
collection_method
random collection of bulk topsoil; each replicate consisted of five subsamples arranged approximately 50 m apart and combined in the field
cur_land_use
not provided
cur_vegetation
native Caatinga dry forest vegetation
isolation_source
preserved Caatinga tropical dry forest soil
link_climate_info
meteorological data obtained from automatic stations of Embrapa Semiarido
local_class
Caatinga dry forest soil
misc_param
soil temperature at site: 42°C; original coordinates: 09°03'0.94"S 40°19'5.96"W
ph
4.2
ph_meth
CaCl2 method; chemical analyses carried out according to Raij et al. (2001)
pool_dna_extracts
no
previous_land_use
preserved Caatinga dry forest; no human intervention for at least 43 years
rel_to_oxygen
not provided
samp_collect_device
sterile plastic bag
samp_mat_process
samples were kept refrigerated during transport, stored at -80°C, and homogenized by sieving through a 2 mm mesh before DNA extraction
season_precpt
rainfall events; wet season peak
season_temp
higher air humidity during May 2015
sieving
2 mm mesh sieve
soil_horizon
A horizon
soil_type
clay soil [ENVO:00002262]
soil_type_meth
Soil type reported in the study as yellow clay; mapped to ENVO clay soil term for BioSample submission.
source_material_id
Caatinga_rainy_P3
store_cond
-80°C
tillage
not applicable
tot_n_meth
extraction of 10 g soil in 50 mL KCl (2 M), according to Keeney and Nelson (1982)
tot_nitro
910 mg/kg
tot_nitro_cont_meth
soil chemical analysis; total N reported for field/season group
tot_org_carb
not collected
water_content
3.1 %
water_content_soil_meth
soil chemical analysis; moisture reported for field/season group
season
rainy season
field_site
Caatinga Experimental Field
site_code
CEF
replicate_site
P3
land_use_category
preserved Caatinga dry forest
agricultural_management
not applicable
sampling_authorization
IBAMA process number 02001.004527/2011-90
sequencing_type
shotgun metagenomic sequencing
dna_extraction_method
PowerMax Soil DNA Isolation Kit (Mo Bio Laboratories)
library_preparation
Nextera DNA Sample Preparation Kit (Illumina-compatible)
sequencing_platform
Illumina HiSeq 2000
read_configuration
paired-end 2 x 100 bp
original_metagenome_name
Caatinga_rainy_P3
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil