Mus musculus
Protocols: Tumor tissues were collected from SCC7-bearing C3H mice following PBS or Fe-Shikonin treatment. Fresh tumor samples were excised and immediately processed for downstream single-cell preparation. Tumor-bearing mice were treated with either phosphate-buffered saline (PBS) or Fe-Shikonin according to the experimental design prior to tumor collection.  Single-cell suspensions were prepared from tumor tissues, and cellular RNA was captured during the droplet-based single-cell RNA library preparation workflow without bulk RNA extraction.  Single-cell RNA libraries were constructed using the DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0 (TaiM 4) according to the manufacturer's instructions. Briefly, single cells were partitioned into droplets, followed by reverse transcription, cDNA amplification, and library construction with sample-specific barcodes.
ENA first public
2026-03-01
INSDC center name
Department of Otorhinolaryngology, Head and Neck Surgery, The First Hospital of Jilin University, Changchun, China
INSDC status
public
Submitter Id
E-MTAB-16487:CON1
broker name
ArrayExpress
cell line
SCC7
collection date
not collected
common name
house mouse
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
tumor
sample name
E-MTAB-16487:CON1
scientific_name
Mus musculus
strain
C3H