Streptococcus sp. KHUD_013
strain
KHUD_013
collection_date
2023-11-01
env_broad_scale
human-associated habitat
env_local_scale
oral cavity
env_medium
Fastidious anaerobe agar (MB cell (cat. MB-F2169)), 0.25% mucin from porcine stomach (sigma cat. M2378), 10% Fetal bovine serum (Gibco cat. 16000044), 5% sheep blood
geo_loc_name
South Korea: Seoul
host
Homo sapiens
isol_growth_condt
Bacterial samples were collected by scraping the buccal mucosa using sterile cotton swabs. The swabs were then soaked in phosphate-buffered saline (PBS) to prepare a bacterial suspension, which was serially diluted. A 200 microL aliquot of the diluted suspension was plated onto Fastidious anaerobe agar supplemented with 5% sheep blood, 0.25% mucin, and 10% fetal bovine serum. The plates were incubated anaerobically at 37 C for 24 hours in an anaerobic chamber containing 10% CO?.., 10% H?.., and a nitrogen-balanced atmosphere.
lat_lon
37.59 N 127.05 E
num_replicons
NA
ref_biomaterial
NA
BioSampleModel
MIGS.ba
BioSampleModel
MIGS/MIMS/MIMARKS.human-oral