Hordeum vulgare
Plants were grown under controlled greenhouse conditions with a 16-hour light/8-hour dark photoperiod at 25 °C during the day and 18 °C at night. Humidity was maintained at 60%, and plants were irrigated weekly with a nutrient solution. Leaf tissue samples were collected from young plants (2-3 weeks old) for DNA extraction. High molecular weight (HMW) DNA was extracted from a single Asplund plant using the MACHEREY-NAGEL NucleoBond HMW DNA kit, following the manufacturer¿s protocol. DNA concentration was assessed using the Qubit 2.0® dsDNA HS assay (Invitrogen, Waltham, USA), and fragment size distribution was evaluated with the Agilent Femto Pulse System (Agilent Technologies, Santa Clara, USA). For HiFi sequencing, the HMW DNA was sheared into ~20 kb fragments using a Megaruptor device (Diagenode) at speed setting 30. HiFi SMRTbell libraries were prepared using the Pacific Biosciences SMRTbell Express Template Prep Kit 3.0. Final libraries were size-selected to a narrow 17-20 kb range using the SageELF system with a 0.75% agarose gel cassette (Sage Science), in accordance with the manufacturer¿s instructions. HiFi circular consensus sequencing (CCS) reads were generated on the PacBio Revio platform (Pacific Biosciences) following standard operating procedures (https://github.com/pacificbiosciences).
biological material ID
HiFi_235_Scarlett
collection date
not collected
sample ID
IPK_LIMS:3203083
biological material ploidy
diploid
material source geographic location
not applicable
material source altitude
not collected
material source description
not collected
plant structure development stage
seedling
biological material DOI
not collected
biological material altitude
112m
biological material geographic location
Germany
geographic location (country and/or sea)
not applicable
plant anatomical entity
leaf
material source longitude
not collected
organism
Hordeum vulgare
air temperature regimen
25°C day/18°C night
biological material coordinates uncertainty
2000m
material source ID
not collected
biological material longitude
11.283
material source DOI
not collected
checklist
ERC000011
infraspecific name
not collected
biological material latitude
51.816
biological material preprocessing
not applicable
IPK sequencing date
2023-08-15
genus
Hordeum
light regimen
16/8-hour light
species
vulgare
material source latitude
not collected
age
2-3 weeks
accession name
not collected