Danio rerio
Protocols: All RNA analyses were performed using triplicates for all conditions using material isolated from the muscle of 30 larvae using Tri-reagent. Total RNA was isolated from dissected injured trunk regions of larvae at 24h post injury (hpi). Tissue was homogenised in TriReagent (Sigma) and total RNA isolated according to the manufacturer's protocol. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library.
ENA first public
2025-09-25
INSDC center name
Kings College London
INSDC status
public
Submitter Id
E-MTAB-15577:Sample 2
age
5
broker name
ArrayExpress
collection date
not collected
common name
zebrafish
developmental stage
larval stage
genotype
Homozygous
geographic location (country and/or sea)
not collected
individual
30
injury
Yes
isolate
not applicable
organism part
skeletal muscle tissue
sample name
E-MTAB-15577:Sample 2
scientific_name
Danio rerio
sex
unknown
strain
tertsa654/1sa6541