Bacteroidales bacterium
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome generated from the metagenomic sample ERS1343347 of study ERP017091.
ENA first public
2026-07-08
INSDC center name
EMG
INSDC status
public
Submitter Id
MGYG000227779
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
missing: third party data
binning parameters
missing: third party data
binning software
missing: third party data
broad-scale environmental context
human-associated habitat
broker name
EBI-EMG
collection date
2013
completeness score
84.51
completeness software
CheckM2_v1.1.0
contamination score
4.12
environmental medium
faeces
geographic location (country and/or sea)
China
geographic location (latitude)
55.67609700
geographic location (longitude)
12.56833700
investigation type
metagenome-assembled genome
isolation_source
human gut metagenome
local environmental context
human gut
metagenomic source
human gut metagenome
project name
AbstractBackgroundInflammatory bowel diseases (IBD), with Crohn's disease (CD) being one of the primary types, have been extensively studied for genetic and gut microbial factors leading to its epidemic in the modern world. However, limited information using metagenomic shotgun-sequencing to pinpoint the gut microbial species and functions involved. Exclusive enteral nutrition (EEN) is a nutritional therapy used for disease-modifying treatment of CD, but its impact on the gut microbiome remains unclear. ResultsWe collected 123 stool samples from 49 CD patients, 14 of whom were resampled after 2 weeks of EEN, and 56 subjects with no disease. Metagenomic shotgun sequencing and analyses confirmed a decreased diversity in the gut microbiota of CD patients, and identified microbial species and potential functions altered in CD, most notably an increased ratio of hexa- to penta-acylated lipopolysaccharide (LPS). Some co-abundance gene groups (CAGs) including Parabacteroides distasonis, Bacteroides intestinalis, Ruminococcus sp. showed strong correlation with clinical indicators such as hs.CRP, Prealbumin, platelets and BMI. The entire cohort could be divided into three types according to their species composition, with Type A largely consisting of healthy controls and Type C containing exclusively CD or after EEN samples. Although compositional changes were generally mild after 2 weeks of EEN, remodeling of the gut microbial network could be observed.ConclusionsOur metagenomic dataset unlock the gut microbiome in CD for functional investigations. Microbiome-based stratification of CD patients before EEN might help optimize the therapy for wider applications.
sample derived from
ERS1343347
sample name
MGYG000227779
scientific_name
Bacteroidales bacterium
sequencing method
missing: third party data
taxonomic identity marker
multi-marker approach