soil metagenome
Composite topsoil sample (P3) collected at 0-10 cm depth from the Bebedouro Experimental Field (BEF) in Petrolina, Pernambuco, Brazil, during the rainy season. This sample represents agriculture-influenced Caatinga fragments and was collected for shotgun metagenomic sequencing of Caatinga tropical dry forest soil microbial communities.
collection_date
2015-05-05
depth
0-10 cm
elev
369 m
env_broad_scale
Caatinga tropical dry forest biome
env_local_scale
Caatinga fragments surrounded by agriculture-influenced irrigated area
env_medium
soil
geo_loc_name
Brazil: Pernambuco, Petrolina
lat_lon
9.138967 S 40.304083 W
agrochem_addition
fertilizer input associated with fertigation practices
altitude
369 m
annual_precpt
below 800 mm/year
collection_method
random collection of bulk topsoil; each replicate consisted of five subsamples arranged approximately 50 m apart and combined in the field
cur_land_use
not provided
cur_vegetation
Caatinga fragments surrounded by agricultural crops
isolation_source
Caatinga soil influenced by agricultural management under fertigation
link_climate_info
meteorological data obtained from automatic stations of Embrapa Semiarido
local_class
Caatinga dry forest soil
misc_param
soil temperature at site: 45°C; original coordinates: 09°8'20.28"S 40°18'14.70"W
ph
5.4
ph_meth
CaCl2 method; chemical analyses carried out according to Raij et al. (2001)
pool_dna_extracts
no
previous_land_use
irrigation perimeter with agricultural crops grown since the 1960s
rel_to_oxygen
not provided
samp_collect_device
sterile plastic bag
samp_mat_process
samples were kept refrigerated during transport, stored at -80°C, and homogenized by sieving through a 2 mm mesh before DNA extraction
season_precpt
rainfall events; wet season peak
season_temp
higher air humidity during May 2015
sieving
2 mm mesh sieve
soil_horizon
A horizon
soil_type
clay soil [ENVO:00002262]
soil_type_meth
Soil type reported in the study as yellow clay; mapped to ENVO clay soil term for BioSample submission.
source_material_id
Bebedouro_rainy_P3
store_cond
-80°C
tillage
not collected
tot_n_meth
extraction of 10 g soil in 50 mL KCl (2 M), according to Keeney and Nelson (1982)
tot_nitro
1050 mg/kg
tot_nitro_cont_meth
soil chemical analysis; total N reported for field/season group
tot_org_carb
not collected
water_content
4.6 %
water_content_soil_meth
soil chemical analysis; moisture reported for field/season group
season
rainy season
field_site
Bebedouro Experimental Field
site_code
BEF
replicate_site
P3
land_use_category
agriculture-influenced Caatinga fragments
agricultural_management
irrigation, fertilization and fertigation practices during the dry season
sampling_authorization
IBAMA process number 02001.004527/2011-90
sequencing_type
shotgun metagenomic sequencing
dna_extraction_method
PowerMax Soil DNA Isolation Kit (Mo Bio Laboratories)
library_preparation
Nextera DNA Sample Preparation Kit (Illumina-compatible)
sequencing_platform
Illumina HiSeq 2000
read_configuration
paired-end 2 x 100 bp
original_metagenome_name
Bebedouro_rainy_P3
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil