Drosophila melanogaster
The following line was used generate the CRISPR-Cas9 mediated knock-in of the iLEXYs tag to the spt5 locus: w[1118]; Pbac{y[+mDint2] = vas-Cas9}VK00027 (vasa-Cas9) (Bloomington stock 51324). Drosophila melanogaster (D. melanogaster) and Drosophila virilis (D. virilis) were maintained in a standard cornmeal-agar medium and grown at 25°C. The spt5-HA-iLEXYs line was always maintained under safelight conditions.