gut metagenome
Cecum contents were collected from indicated groups of mice (n=3) aseptically, snap frozen, stored in -80 C and later used for the meta-genomic analysis. QIAamp Power FecalPro DNA Kit was used for microbial DNA isolation from caecum contents followed by quantification using Qubit dsDNA High Sensitivity Assay.  The V3-V4 region of 16S rRNA gene was utilised for amplicon sequencing using paired-end chemistry (2300 bp), generating high yield reads. The sequenced reads were overlapped and stitched to form long reads greater than 400 bp using join pairs program of QIIME2. The stitched reads were subjected to chimera removal and quality filtering using VSEARCH2 program.