s08T1923
freshwater metagenome
ENA first public
2025-04-28
INSDC center name
IOW Leibniz-Institut fur Ostseeforschung Warnemuende
INSDC status
public
Submitter Id
182875:5029d317-867f-47ef-8dc3-679b6e5cb489
broker name
GFBIO
collection date
2023-05-11
comment
sequences were generated in mixed orientation, separate read files submitted for each orientation (here: R1 file contains forward reads)
environment (biome)
freshwater biome [ENVO:00000873]
environment (feature)
fresh water [ENVO:00002011]
environment (material)
freshwater river [ENVO:01000297]|microbial community [PCO:1000004]
environmental package
water
environmental_sample
yes
experiment_id
s08T1923_lib1
geographic location (country and/or sea)
Germany
geographic location (depth)
0
geographic location (elevation)
0
geographic location (latitude)
54.08414944
geographic location (longitude)
12.15100642
geographic location (region and locality)
Muehlendamm
investigation type
mimarks-survey
nucleic acid extraction
DNA was extracted from the samples using the magMAX Wastewater Kit (cat. nr. A52606) according to the protocol using the KingFisher Flex system after 10 sec of bead beating with silica beads.
project name
OTC-Genomics
sample material processing
Samples were taken with a metal bucket and transported to the research institute in a glass bottle on ice. 500 ml of the sample was filtered onto a 0.22 um pore-size polycarbonate filter (Durapore PVDF Filter, 47 mm diameter, Darmstadt, Germany) with 300-400 mbar, shock frozen in liquid nitrogen and stored in a test tube at -80 degrees Celsius until DNA extraction.
sample name
182875:5029d317-867f-47ef-8dc3-679b6e5cb489
sample_description
Surface water sample taken at Muehlendamm (54.0841494398656, 12.151006424457755) on 2023-05-11 (YYYY-MM-DD), part of sequencing batch 18.
scientific_name
freshwater metagenome
submitted to insdc
true
water environmental package
water