Sphaerosorus coelastroides
strain
none
collection_date
2023-07
depth
upper 1 meter
env_broad_scale
freshwater river in the Appalachian Mountains of Virginia, USA
env_local_scale
mid river
env_medium
plankton
geo_loc_name
USA: Russell County, Virginia
isol_growth_condt
microalgal samples were collected from the Clinch River near Saint Paul (Russell County), Virginia using a 20 m plankton net. The samples were transported to a laboratory at the University of Virginias College at Wise, where they were centrifuged at 3000xg for 10 minutes in 250 mL bottles. The pelleted cells were resuspended in a small volume of the remaining river water. Aliquots of 25, 50, or 100 L were spread onto agarose plates containing 1/4x Bolds Modified Basal Freshwater Nutrient Solution (Sigma-Aldrich, Saint Louis, MO), Hutners trace elements (Harris 2009), Sodium Silicate (275 mg/L, 2.25 mM) and Tris HCl (0.2 g/L), dissolved in Clinch River water passed through a 0.45m filter, and adjusted to pH 7. Plates were incubated under LED growth lights (AC Infinity ION Frame EVO3, City of Industry, CA), daylight cycle 16 hrs L/ 8 hrs D, 170M/m-2 s-1 at room temperature.
lat_lon
36.53 N 82.17 W
BioSampleModel
MIGS.eu
BioSampleModel
MIGS/MIMS/MIMARKS.water