Unsorted
Mus musculus
strain
NIH Swiss
age
NA
collection_date
NA
geo_loc_name
USA
sex
NA
tissue
NA
cell_line
MNK-3
cell_subtype
MNK3i
sample_type
Cell Culture
treatment
MNK3i cells were transduced with a CRISPRi lentiviral gRNA library targeting 20K genes, selected for guide integration with puromycin (2ug/ml), treated with doxycycline (2ug/ml) for 48hr to induce dCas9-KRAB expression, and then stimulated with IL-1b and IL-23 (10ng/ml each) for 21hr. Cells were stained for intracellular IL-22 and sorted by flow cytometry into IL-22 negative, low, or high populations. This is the unsorted sample.
BioSampleModel
Model organism or animal