Homo sapiens
Protocols: HEK293T were purchased from ATCC and were grown in DMEM supplemented with 10% FBS. HEK293T cells were co-transfected with plasmids LV-mCherry-T2A-Cas13a and LV-sgRNA-13a-GFP-Neo or LV-sgRNA-13a-GFP-MALAT1 using Polyfect (Qiagen, 301105) following the manufacturer's protocol. LwaCas13a expression was induced by adding doxycycline (Sigma D9891) to the cells growing in media supplemented with TET-free foetal bovine serum (Takara, 631107) for 48 h. Total RNA isolation was done using HP RNA isolation kit (Roche E3101K) following manufacturer's instructions. Illumina stranded mRNA library
ENA first public
2025-05-29
INSDC center name
UOM-BCF
INSDC status
public
Submitter Id
E-MTAB-14334:Sample 2
broker name
ArrayExpress
cell_line
HEK293T
cell_type
immortalized human embryonic kidney cell
collection date
not collected
common name
human
genetic construct
MALAT1
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
kidney
sample name
E-MTAB-14334:Sample 2
scientific_name
Homo sapiens
sex
female