Mus musculus
B16 CELL LINE
strain
C57BL/6
isolate
B16
breed
mouse
cultivar
N/A
ecotype
N/A
age
N/A
dev_stage
N/A
collection_date
N/A
geo_loc_name
N/A
sex
N/A
tissue
N/A
biomaterial_provider
ATCC
birth_date
N/A
birth_location
N/A
breeding_history
N/A
breeding_method
Inbreeding
cell_line
B19 melanoma cell line
cell_subtype
None specified
cell_type
Melanoma cell
collected_by
zhaoruoyang
culture_collection
ATCC:CRL-6322
death_date
N/A
disease
Malignant melanoma
disease_stage
Primary malignant melanoma
genotype
WT
growth_protocol
Thaw frozen B16 cells in a 37°C water bath.Transfer cells to a culture dish with DMEM medium (10% FBS, 1% penicillin-streptomycin).Incubate at 37°C with 5% CO₂.Replace medium every 2-3 days.Subculture at 80-93% confluence using trypsin-EDTA.
health_state
Viable
isolation_source
C57BL/6 mouse skin melanoma
lat_lon
28 N 120 W
phenotype
Malignant melanoma cells
sample_type
Cultured tumor cell line
specimen_voucher
CRL-6322
store_cond
B16 cells are stored in liquid nitrogen (-150°C to -196°C) or at -80°C. The freezing medium typically contains 5–10% DMSO with 90–95% complete culture medium or fetal bovine serum (FBS). After thawing, cells are cultured at 37°C with 8% CO₂
stud_book_number
N/A
treatment
add nothing
BioSampleModel
Model organism or animal