soil metagenome
assay
ITS1F + ITS2R
plot
GRASS3
distance_direction_from_center_point
2 m from center NW
collection_date
2022-04
depth
0-2
elev
1500.8
env_broad_scale
montane grassland biome[ENVO_01000194]|temperate grassland biome[ENVO_01000193]
env_local_scale
montane grassland biome[ENVO_01000194]|temperate grassland biome[ENVO_01000193]
env_medium
soil environment[ENVO_01001044]
geo_loc_name
USA: Arizona, Fort Huachuca, FH South Range
lat_lon
31.50554 N 110.30382 W
annual_precpt
360.7 mm
cur_vegetation
desert grassland
ph
1.1398837685e-06
ph_meth
H2O extracted
pool_dna_extracts
Six replicates of each sample were extracted and then pooled together into one sample before amplification. We wanted to increase the likelihood of capturing eDNA.
samp_size
Approx. 30 ml of soil
samp_vol_we_dna_ext
Approx. 0.25 g
soil_text_measure
% Sand 65.092, % Silt 22.956, % Clay 11.902, Sandy Loam
soil_texture_meth
Malvern MasterSizer
soil_type
Paleustalfs
store_cond
Samples were stored in 30mls of RNA later and kept at 4ºC until they were extracted.
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil