Equus caballus
Protocols: Muscle samples were obtained from each horse standing at rest without sedation. Sample collection was performed between 7:30 am and 11:30 am, when all the horses were at rest. Approximately 300 mg muscle tissue was collected from the ventral compartment of the middle gluteal muscle (Valette et al. 1999). After collection, the muscle samples were stored in RNAlater (Thermo Fisher Scientific, Waltham, MA, USA) at 4°C for 24 hours and then transferred to a freezer at −20°C prior to RNA extraction.  RNA extraction, quantification and quality control (QC) was performed as previously described (Bryan et al. 2017; Farries et al. 2019). Briefly, approximately 70 mg of muscle tissue underwent nucleic acid extraction with TRIzol reagent (Thermo Fisher Scientific) followed by DNase (RNase free) treatment (Qiagen, Hilden, Germany).  RNA sequencing was conducted by the Research Technology Support Facility at Brigham Young University and the Research Technology Support Facility at Michigan State University. The TruSeq Stranded mRNA Library Preparation Kit LT (Illumina, San Diego, USA) was used to build the strand-specific and indexed Illumina sequencing libraries, which were pooled, with 18–20 indexed libraries per pool, and sequenced on an Illumina HiSeq 2500 platform using Rapid Run flow cells and reagents (Illumina).
ENA first public
2026-05-20
INSDC center name
UCD
INSDC status
public
Submitter Id
E-MTAB-15170:Sample 1
age
2
broker name
ArrayExpress
collection date
not collected
common name
horse
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
middle gluteal muscle
sample name
E-MTAB-15170:Sample 1
scientific_name
Equus caballus
sex
female