Sindbis virus
SINV-mCherry FMV2A viral RNA was extracted from Baby Hamster Kidney (BHK-21) cell culture. This construct, derived from Sindbis virus strain TE12, contains the mCherry gene followed by the Foot-and-Mouth Virus 2A element, inserted between the capsid and E3 in the SINV TE12 cDNA clone (Sokoloski et al., 2013). The construct was transcribed in vitro and transfected into BHK-21 cells, where the resulting virus was collected, passaged, and plaqued. A plaque-purified isolate was then used for reinfection of BHK-21 cells, followed by RNA extraction and sequencing via Illumina technology.
strain
TE12 mCherry FMV2A
collection_date
2024-10-19
env_broad_scale
n/a
env_local_scale
n/a
env_medium
Schneider's Insect Medium
geo_loc_name
n/a
host
Baby Hamster Kidney (BHK-21) cell culture
isol_growth_condt
RNA extracted from host cell lysate
lat_lon
n/a
propagation
budding
BioSampleModel
MIGS.vi
BioSampleModel
MIGS/MIMS/MIMARKS.human-associated