Caenorhabditis elegans
Protocols: The worms were cultivated on 8P agar plates coated with NA22 E. coli at 20 °C, 750 worms were isolated after treatment for RNA isolation.  RNA was isolated from 750 worms in 1 mL TRI reagent® (Sigma-Aldrich). Isolated RNA was purified with the RNA clean and concentrator™-5 Kit (Zymo Research) according to the manufacturers instructions.  Library preparation was performed with 300 ng RNA using the NEBNext® Ultra™ II directional RNA library prep kit for Ilumina® with 14 PCR cycles according to manufacturers instructions.
ENA first public
2025-06-14
INSDC center name
Molecular Cell Biology
INSDC status
public
Submitter Id
E-MTAB-15180:Sample 9
age
adult
broker name
ArrayExpress
collection date
not collected
developmental stage
D2
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
750 individuals
isolate
not applicable
organism part
whole worm
sample name
E-MTAB-15180:Sample 9
scientific_name
Caenorhabditis elegans
sex
hermaphrodite
stimulus
24hFAC+24hFAC+DEM
strain
Bristol N2