Mus musculus
Protocols: BMEL cells were trypsinized and permeabilized with NE1 buffer as per https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3. BMEL cells were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, 10 µg/mL insulin, 30 ng/mL IGF-II, and 50 ng/mL EGF, at 37 °C under 5% CO2. Permeabilized cells were treated for Cut & Tag experiments as per https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3. Libraries were PCR amplified for Illumina sequencing using primers described in https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3
ENA first public
2025-06-14
INSDC center name
I2BC
INSDC status
public
Submitter Id
E-MTAB-15189:TKO_H3-K9me3
broker name
ArrayExpress
cell line
bipotential mouse embryonic liver
cell type
bipotential mouse embryonic liver
collection date
not collected
common name
house mouse
developmental stage
mouse embryo stage
disease
normal
genotype
Cbx1-/-,Cbx3-/-,Cbx5-/-
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
liver
sample name
E-MTAB-15189:TKO_H3-K9me3
scientific_name
Mus musculus