Homo sapiens
Protocols: Blood was collected in Tempus tubes from subjects, and Tempus tubes were stored at -80deg until RNA extraction. We performed gene expression analysis of dengue seropositive versus dengue seronegative subjects. RNA extraction from whole blood was performed using the Tempus Spin RNA Isolation kit (Thermo Scientific). RNA was eluted in 90ul of RNase-free water. 1 μg total RNA was used for following library preparation. The poly(A) mRNA isolation was performed using Oligo(dT) beads. The mRNA fragmentation was performed using divalent cations and high temperature. Priming was performed using Random Primers. First strand cDNA and the second-strand cDNA were synthesized. The purified double-stranded cDNA was treated to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. Size selection of Adaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated.
ENA first public
2025-09-09
External Id
SAMEA114497882
INSDC center name
Duke-NUS Medical School
INSDC last update
2023-10-13T16:10:19Z
INSDC status
public
Submitter Id
E-MTAB-13429:DBL-16
age
34
broker name
ArrayExpress
clinical history
dengue seropositive
collection date
not collected
common name
human
developmental stage
adult
disease
normal
geographic location (country and/or sea)
not collected
individual
DBL-16
isolate
not applicable
organism part
blood
sample name
E-MTAB-13429:DBL-16
scientific_name
Homo sapiens
sex
female