Escherichia coli
This sample represents an enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain EDL933 with a targeted deletion of the stx2 gene. The mutant was generated using a CRISPR-Cas9 allelic exchange system without insertion of an antibiotic resistance cassette, and was validated by PCR. The genome was sequenced using PacBio HiFi (Revio) long-read technology.
strain
EDL933 stx2 deletion
collected_by
Han Young Chung
collection_date
2024-07
geo_loc_name
South Korea: Daejeon
subsrc_note
engineered mutant
host
Homo sapiens
host_disease
Hemolytic uremic syndrome
isolation_source
laboratory-derived mutant
lat_lon
36.37 N 127.36 E
mutation
stx2 deletion (Shiga toxin 2 gene knockout)
parent_strain
Escherichia coli O157:H7 EDL933
construction_method
CRISPR-Cas9 mediated allelic exchange (no antibiotic resistance cassette)
BioSampleModel
Pathogen.cl