Mus musculus
Protocols: Seven-month-old male C57BL/6J mice were fed either a low fat diet (LFD; 10 kcal% fat; D12450J, Research Diets) or a high fat diet (HFD; 60 kcal% fat; D12492i, Research Diets) for 20 weeks. At study termination, mice were euthanized and quadriceps muscles were dissected and immediately flash-frozen in liquid nitrogen. Fresh-frozen quadriceps were cryosectioned into 10 µm cross-sections at the Helmholtz Munich Core Facility Pathology and Tissue Analytics and placed on 10x Genomics Visium spatial gene expression slides. Sections were fixed and stained with DAPI, rat anti-Laminin (1:200, ab11576, Abcam), and rabbit anti-Perilipin (1:100, #57117SF, Cell Signaling), detected with donkey anti-rabbit AlexaFluor555 (1:100, A31572, Invitrogen) and goat anti-rat AlexaFluor647 (1:100, A21247, Invitrogen). Whole-section fluorescence images were acquired on a Zeiss AxioScan 1 slide scanner at 20x magnification. Visium Spatial Gene Expression Slide & Reagent Kit, 4 rxns, PN-1000187 10x Genomics Visium Spatial Gene Expression 3′ barcoded cDNA library-construction workflow for fresh-frozen tissue
ENA first public
2026-07-12
INSDC center name
Computational Discovery Research Group; Institute for Diabetes and Obesity; Helmholtz Munich
INSDC status
public
Submitter Id
E-MTAB-17271:Sample_24L008228
age
7
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
diet
HighFatDiet
genotype
WT
geographic location (country and/or sea)
not collected
individual
24L008226
isolate
not applicable
organism part
quadriceps
sample name
E-MTAB-17271:Sample_24L008228
scientific_name
Mus musculus
sex
male
strain
C57BL/6J