wastewater metagenome
isolation_source
wastewater
geo_loc_name
USA: Pennsylvania, Philadelphia
collected_by
Philadelphia Water Department
sequenced_by
Philadelphia Public Health Laboratory
purpose_of_ww_sampling
public health surveillance community-level
purpose_of_ww_sequencing
SARS-CoV-2 surveillance
collection_site_id
NE WPCP
isolate
WW-2025-09-02-NorthEast
specimen_processing_id
WW-2025-09-02-NorthEast-Rep3
specimen_processing
biological replicate
specimen_processing_details
Composite sample was aliquoted into 10 mL biological replicates before concentration, extraction, and sequencing (see specimen_processing_id)
concentration_method
ceres nanotrap
extraction_method
thermo magmax wastewater ultra nucleic acid isolation kit
extraction_control
murine hepatitis virus
project_name
CDC NWSS
ww_surv_system_sample_id
WW-250901-250903-NE
ww_sample_site
wastewater treatment plant
ww_sample_matrix
raw wastewater
ww_sample_type
composite
ww_sample_duration
24
ww_surv_jurisdiction
PH
ww_population
1004057
ww_surv_target_1
SARS-CoV-2
ww_surv_target_1_gene
N gene (N2)
ww_surv_target_1_known_present
yes
ww_surv_target_1_conc
68084.36
ww_surv_target_1_conc_unit
copies/L wastewater
ww_surv_target_1_protocol
ddPCR detection of SARS-CoV-2 using the BioRad PREvalence Kit on the BioRad QX200
ww_surv_target_2
SARS-CoV-2
ww_surv_target_2_gene
E gene (orf4)
ww_surv_target_2_known_present
yes
ww_surv_target_2_conc
41334.11
ww_surv_target_2_conc_unit
copies/L wastewater
ww_surv_target_2_protocol
ddPCR detection of SARS-CoV-2 using the BioRad PREvalence Kit on the BioRad QX200
gisaid_accession
EPI_ISL_20331766
gisaid_virus_name
hCoV-19/env/USA/PA-PHL2-VH-Merged-G02-0912/2025
collection_date
2025-09-01
BioSampleModel
SARS-CoV-2: wastewater surveillance