viral metagenome
HtPIP 0.2 micron plate was seeded with overnight E. coli diluted in LB, floated on top of unfiltered wastewater, and incubated for 3 days at 20C shaking at 50 rpm.  Resulting lysate was filtered and combined with fresh E. coli overnight culture, top agar and plated.  Resulting plaque lawns were harvested by incubating with SM buffer, scraping, centrifuging and filtering (0.2 micron).
host
E. coli MG1655
isolation_source
Untreated municipal wastewater
collection_date
2024-04-18
geo_loc_name
USA: California, Livermore
lat_lon
37.691012 N 121.806394 W
samp_size
5 ul
BioSampleModel
Metagenome or environmental