Mus musculus
Protocols: Mice were infected with Candida albicans as described in the main manuscript. At the indicated timepoints (naive, day 1, day 2 post-infection), tissues (tongue) were harvested, mechanically and enzymatically dissociated into single-cell suspensions, and filtered through a 40 µm cell strainer. Cell viability was assessed  prior to downstream processing. Single-cell suspensions were processed directly for library preparation. Cells were counted and viability confirmed (>85%) prior to loading onto the Chromium platform. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium platform (Single Cell 3′ Gene Expression, v3/v3.1 chemistry) according to the manufacturer's instructions. Briefly, single cells were encapsulated into droplets with barcoded gel beads to generate Gel Bead-in-Emulsions (GEMs), followed by reverse transcription, cDNA amplification, and library construction.
strain
C57BL/6J
organism
Mus musculus
organism
Mus musculus
collection date
not collected
sex
pooled male and female
scientific_name
Mus musculus
common name
house mouse
organism part
tongue
developmental stage
adult
genotype
wild type genotype
isolate
not applicable
age
8
geographic location (country and/or sea)
not collected
infect
naive