Homo sapiens
Protocols: Total RNA was isolated from human primary microvascular endothelial cells (HPMECs) seeded on 6 well plates (3.8 x 105cells/well). HPMECs were infected with influenza and treated with DMSO, Ap (1 μM) or Ap-1 (a modified inactive derivative of Ap) for two hours.  Lysates were prepared by washing cells twice with PBS+, lysed in recommended volume of RLT Lysis buffer from the Qiagen RNeasy RNA Isolation Kit (Cat. No. / ID: 74104) and collected by scraping with a sterile cell scraper. Lysates were vortexed for 20 seconds, centrifuged at max speed for 3 minutes at room temperature at stored at -80C for up to two weeks prior to extraction with the kit which was performed according to manufacturer's instructions.  Library prep was performed using the NEB Ultra II Directional mRNA kit.
ENA first public
2025-06-14
INSDC center name
University of Toronto, St. Michael's Hospital
INSDC status
public
Submitter Id
E-MTAB-15177:Ap no Flu 2
broker name
ArrayExpress
cell line
primary cell line
cell type
endothelial cell
collection date
not collected
common name
human
developmental stage
adult
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
endothelium
sample name
E-MTAB-15177:Ap no Flu 2
scientific_name
Homo sapiens