KK1904_141_08_ATF_pre
Cricetulus griseus
Chinese hamster lung (CHL)-YN cells (RIKEN Bioresource Cell Bank, RCB5004), serum-free suspension, 2 days post-seeding, antibody-producing, scRNA-Seq 71 cells analyzed
sample_name
KK1904_141_08_ATF_pre
age
Passage 153, 2 days in culture
cell_line
CHL-YN
collection_date
2023-01-18
geo_loc_name
Japan:Osaka
growth_condition
Viable cell density: 16.7 x 10^6 cells/mL, Viability: 99.5%
growth_protocol
CHL-YN cells were cultured in 20 mL of EX-CELL Advanced HD Perfusion Medium (MERCK, 24370C) supplemented with 1 mL of L-Glutamine (200 mmol/L stock; final concentration, 10 mM; FUJIFILM Wako, 073-05391) and 10 uL of Puromycin (10 mg/mL stock; final concentration, 5 ug/mL; InvivoGen, ant-pr-1) in a 125 mL flask. The flask was shaken (140 rpm) at 37 degree C in a humidified 5% CO2 atmosphere (80% relative humidity).
sex
not applicable
single_cell_identifier
KK1904_141_08_ATF_pre
strain
CHL-YN
tissue
lung