Sample 27
Homo sapiens
Protocols: Peripheral blood samples were collected from patients who underwent bone mineral density (BMD) testing at the People's Hospital of Gansu Province. The cohort included 15 patients with osteoporosis combined with diabetes and 15 control subjects. Osteoporosis was defined as a spinal BMD T-score ≤ -2.5 SD, while the control group had a T-score ≥ -1.0 SD. Total RNA was isolated and purified from peripheral blood samples using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). The quantity and integrity of the extracted total RNA were assessed prior to library construction. The RNA library was prepared following these steps: mRNA with PolyA tails was captured and fragmented. First-strand cDNA was synthesized using reverse transcriptase, followed by second-strand synthesis using E. coli DNA polymerase I (NEB, Cat. No. M0209) and RNase H (NEB, Cat. No. M0297). The double-stranded DNA was then purified, and the fragment size was selected. The UDG enzyme (NEB, Cat. No. M0280) was used for digestion. Finally, PCR amplification was performed to generate sequencing libraries with a fragment size of 300 bp ± 50 bp.
ENA first public
2026-01-21
INSDC center name
Gansu Provincial People's Hospital
INSDC status
public
Submitter Id
E-MTAB-16559:Sample 27
broker name
ArrayExpress
collection date
not collected
common name
human
disease
osteoporosis with diabetes
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blood
sample name
E-MTAB-16559:Sample 27
scientific_name
Homo sapiens