Sample 11
Homo sapiens
Protocols: NK-92, CAR NK-92, hydrocortisone-treated CAR NK-92, NR3C1-knockout CAR NK-92, and hydrocortisone-treated NR3C1-knockout CAR NK-92 cells were co-cultured with CEACAM5⁺ A549 target cells for 16 hours. After co-culture, NK-92–derived cells were collected by FACS sorting. Sorted cell pellets were immediately processed for RNA extraction. Total RNA was extracted using the Qiagen RNA extraction kit following the manufacturer's instructions, including optional on-column DNase digestion. RNA concentration and purity were assessed using NanoDrop. High-quality RNA was used for subsequent library preparation by Novogene.  RNA-seq libraries were generated by Novogene using their standard mRNA-seq workflow.
ENA first public
2025-12-19
INSDC center name
PhD student, department of Pathology, University of Cambridge, UK
INSDC status
public
Submitter Id
E-MTAB-16373:Sample 11
broker name
ArrayExpress
cell line
NK-92
cell type
natural killer cell
collection date
not collected
common name
human
genetic modification
CEACAM5 specific CAR expression
genotype
CEACAM5-CAR
geographic location (country and/or sea)
not collected
growth condition
Cocultured with CEACAM5 expressing A549 cells
isolate
not applicable
sample name
E-MTAB-16373:Sample 11
scientific_name
Homo sapiens