iEHM-TC1133-3
Homo sapiens
Protocols: Tissues were placed in 2 mL collection tube and were washed twice with PBS. Tissues were directly put into liquid nitrogen to be snap frozen. No dissction were applied to the samples. Day 40 SNO and day 28 EHM were fused for 6 weeks generating the iEHM which was supplemented with a 1:1 mixture of Basal medium and EHM medium. Individual EHM and SNO were also cultured individually with the iEHM 1:1 media for the same duration as the iEHM. No treatments were given to the tissues. Sequence images were transformed with BaseCaller Illumina software to BCL files and demultiplexed to fastq files with bcl2fastq v2.20.0.422. Sequencing quality was determined using FastQC v. 0.11.5 software RNA from frozen tissues were harvested using the Promega ReliaPrep RNA Tissue Miniprep system and was cleaned with the RNA Clean & Concentrator.  RNA quality was assessed by measuring the RNA integrity number (RIN) using a Fragment Analyzer HS Total RNA Kit (Advanced Analytical Technologies, Inc.). Library preparation for RNA-Seq was performed in the STAR Hamilton NGS automation using the Illumina Stranded mRNA Prep (Cat.N° 20040534) and the ID for Illumina RNA UD Indexes Set A, Ligation with 96 Indexes (Cat. N° 20091646) starting from 300 ng of total RNA. The size range of the final cDNA libraries was determined by applying the SS NGS Fragment 1- to 6000-bp Kit on the Fragment Analyzer (average 340 bp). Accurate quantification of cDNA libraries was performed by using the DeNovix DS-Series System.
disease
normal
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
scientific_name
Homo sapiens
cell type
induced pluripotent stem cell
common name
human
organism part
umbilical cord blood
developmental stage
embryo
genotype
somatic genotype
isolate
not applicable
growth condition
iEHM
cell line
RUCDRi002-A
geographic location (country and/or sea)
not collected