KO
Mus musculus
Protocols: Cells were isolated from the trachea of three male mice for each genotype of WT, HET, and KO. Trachea epithelial cells were isolated enzymatically in Ham's F12, supplemented with 1% (v/v) Penicillin-Streptomycin, 2 mg/ml dispase, 1 U/mL DNase I, overnight at 4 Celsius degree. After overnight enzymatic digestion, FBS was added to the tubes with trachea to inactivate dispase. Tubes were inverted 15 times to dislodge epithelial cells. nucleic acid extraction was done according to 10X 3' version 3 protocol nucleic acid library construction was done according to 10X 3' version 3 protocol
ENA first public
2026-06-18
INSDC center name
University Medical Center Groningen
INSDC status
public
Submitter Id
E-MTAB-17081:KO
age
8
broker name
ArrayExpress
cell type
epithelial cell
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Homozygous KO
geographic location (country and/or sea)
not collected
individual
3
isolate
not applicable
organism part
trachea
sample name
E-MTAB-17081:KO
scientific_name
Mus musculus
sex
male
strain
C57NL/6J