3 day/ 1 biol. rep. / 2 tech. rep / WOX2 positive / R2
Arabidopsis thaliana
Protocols: The upper part of the explants after 3 days of somatic embryogenesis culture was taken for nuclei isolation. Arabidopsis was grown on soil under a 16h-light/8h-dark regime at 21C Somatic embryogenesis was induced by Gaj 2001 protocol with 4,5uM of 2,4-D in the medium. RNA was extracted from FANSed nuclei with Trizol LS (Ambion). cDNA was synthesized using SMARTER technology (clontech).
ENA first public
2026-01-21
INSDC center name
University of Silesia in Katowice Faculty of Natural Sciences | Institute of Biology, Biotechnology and Environmental Protection
INSDC status
public
Submitter Id
E-MTAB-16183:3 day/ 1 biol. rep. / 2 tech. rep / WOX2 positive / R2
age
3rd day of somatic embryogenesis
broker name
ArrayExpress
cell type
WOX2-positive
collection date
not collected
common name
thale cress
developmental stage
embryogenic
genotype
pWOX2:H2B-GFP
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
cotyledon
sample name
E-MTAB-16183:3 day/ 1 biol. rep. / 2 tech. rep / WOX2 positive / R2
scientific_name
Arabidopsis thaliana
ecotype
Col-0