GA36D20_2
Drosophila melanogaster
Protocols: Heads from adult female flies were snap frozen in liquid nitrogen and kept at -80°C before proceeding with RNA extraction RNA extraction: Fly heads were separated from the bodies by mechanical force, 15 heads per sample were placed in cold 2 mL screw cap tubes (Sarstedt) containing 425-600 μm acid-washed glass beads (Merck, Cat No. G8772). 1 mL of cold TRIzol™ reagent (ThermoFisher, Cat No. 15596026) was added to each tube. The tubes were ribolysed at maximum speed for 15 seconds twice and then incubated for 5 minutes at room temperature. After that, 200 μL of chloroform (Fisher Scientific, Cat No. 15668480) was added to each tube, followed by vortexing for 15 seconds and incubation for 2 minutes at room temperature. Samples were centrifuged at full speed for 15 minutes. After centrifugation, the upper colourless phase was collected and transferred into a new Eppendorf tube. Samples were incubated with 500 μL isopropanol (ThermoFisher, Cat No. P/7500/15) for 15 minutes at room temperature, followed by 15 minutes of centrifugation at 4°C at full speed. The RNA pellet was washed twice with 70% ethanol before resuspending in 30 μL nuclease-free water (Ambion, AM9937). The concentration of total RNA purified for each sample was measured using a Nanodrop 2000c spectrophotometer (Thermo Scientific). The samples were sent to Novogene (UK) Co., Ltd in Cambridge for sequencing. Triplicate samples were prepared for each condition. The company assessed RNA quality and quantity using the 2100 Bioanalyzer Instrument (Agilent) with the RNA Nano Chip (Agilent). Libraries were made on Illumina sequencing PE150 and sequenced as 150 bp paired-end reads, with the mRNA library preparation made with poly A enrichment, resulting in 20 million reads per sample.
ENA first public
2026-05-24
INSDC center name
Institute of Healthy Ageing, UCL
INSDC status
public
Submitter Id
E-MTAB-16995:GA36D20_2
age
20
broker name
ArrayExpress
collection date
not collected
common name
fruit fly
developmental stage
adult
disease
FTDALS
genotype
UAS-GA36; nSybGS
geographic location (country and/or sea)
not collected
individual
GA36D20_2
isolate
not applicable
organism part
head
sample name
E-MTAB-16995:GA36D20_2
scientific_name
Drosophila melanogaster
sex
female
strain
w1118