2D-iPSC-cortical-neurons_D13
Homo sapiens
Protocols: The iPSC-derived brain cells were trypsinized and dissociated using 0.5% trypsin in PBS, and then resuspended in 0.04% BSA in PBS. A 40-μm strainer was used to remove cell debris and clumps, to obtain single-cell suspension. Nucleic acids were extracted following the 10x Genomics Chromium Single Cell 5' Reagent Kits v2 User Guide, up to the cDNA synthesis step. After GEM (Gel Bead-in-Emulsion) generation and reverse transcription, emulsions were broken, and barcoded cDNA was purified using silane magnetic beads, according to the manufacturer's instructions, with a target recovery of 10,000 cells per sample. Single-cell transcriptomic libraries were generated following the Chromium Single-cell 5' Reagent Kits V2 User Guide.
ENA first public
2026-02-18
INSDC center name
Faculty of Medicine Siriraj Hospital, Mahidol University
INSDC status
public
Submitter Id
E-MTAB-15054:2D-iPSC-cortical-neurons_D13
broker name
ArrayExpress
cell type
neuron
collection date
not collected
common name
human
developmental stage
Day 13
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
brain
progenitor cell type
iPSC
sample name
E-MTAB-15054:2D-iPSC-cortical-neurons_D13
scientific_name
Homo sapiens