AU5
Homo sapiens
Protocols: A miRNA analysis on peripheral blood mononuclear cells (PBMCs) was performed by RNA-seq in 27 subjects, including 12 ASD patients and 15 healthy controls. The subjects were recruited at the Oasi Research Institute - IRCCS (Troina, Italy) and originated from the same region (Sicily) to minimize environmental influences.  Total RNA was isolated from peripheral blood mononuclear cells (PBMCs) using the TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA), following the manu-facturer's protocol. RNA concentration and purity were assessed with the NanoDrop™ 2000/2000c (Thermo Fisher Scientific, Waltham, MA, USA), whereas RNA integrity was evaluated with the Agilent TapeStation 4200 (Agilent Technologies, Santa Clara, CA, USA) using the RNA ScreenTape Assay 2.7. Extracted RNA was stored at −80 °C until further use. Indexed libraries were generated from 250 ng of total RNA using the QIAseq miRNA Li-brary Kit (Qiagen), according to the manufacturer's guidelines. Library quality and con-centration were evaluated with the Agilent TapeStation 4200 and Qubit fluorometer (Invi-trogen), respectively.
ENA first public
2026-05-11
INSDC center name
Genomix4Life
INSDC status
public
Submitter Id
E-MTAB-16206:AU5
age
not available
broker name
ArrayExpress
cell type
mononuclear cell
collection date
not collected
common name
human
disease
autism spectrum disorder
geographic location (country and/or sea)
not collected
individual
20
isolate
not applicable
organism part
peripheral blood
sample name
E-MTAB-16206:AU5
scientific_name
Homo sapiens