L100342_Track-149292
Mus musculus
Protocols: Testes were detunicated and seminiferous tubules were dissociated in 1 mL HBSS containing Collagenase A (25 mg/mL), Dispase II (25 mg/mL) and DNase I (2.5 mg/mL) for 30 min at 37 °C with intermittent mixing. Digestion was quenched with 4 mL DMEM + 10% FCS, cells were pelleted (400 rcf, 5 min, RT), washed in DPBS-S (PBS + 10% knockout serum), and filtered through a 40 µm mesh. For dead-cell removal, the DPBS-S wash and filtration step was repeated, followed by differential-speed centrifugation (400/200/100 rcf) to enrich for viable cells. Cells were incubated with anti-ACRV1-PE (30 min, RT), washed, incubated with anti-PE magnetic microbeads (30 min, RT), and subjected to negative magnetic selection on Miltenyi MS columns to collect the viable, unlabeled flow-through. After a final wash and pellet, cells were resuspended in PBS + 0.04% BSA and labelled with Cell Multiplexing Oligos (CMOs) according to the manufacturer's instructions. Dead cell removal was done on two WTs Nucleic acid extraction was performed as part of the library construction.
ENA first public
2026-06-18
INSDC center name
Technische Universität Dresden
INSDC status
public
Submitter Id
E-MTAB-16317:L100342_Track-149292
broker name
ArrayExpress
cell type
Testes cell suspension
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
testis
sample name
E-MTAB-16317:L100342_Track-149292
scientific_name
Mus musculus