Skin-H
Homo sapiens
Protocols: This study was approved by the Ethics Committee of Shuguang Hospital (No.2024-1488-071-01 and No.2024-1598-181-01). Written informed consent was obtained from all participants. Hypertrophic scar tissue was collected during elective plastic surgery. Rhinophyma specimens were harvested via five-blade scarification surgery. Normal skin was acquired from donors undergoing routine circumcision. Each sample measured approximately 0.5 cm³ (normal skin: 0.25 cm³, two samples per donor). Patients had received no prior topical/systemic treatments affecting the sampled areas for ≥4 weeks before surgery. All procedures complied with the Declaration of Helsinki. Tissues were minced and enzymatically digested with 0.75mg/mL collagenase I, 2mg/mL collagenase IV, 0.2mg/ml hyaluronidase and 75 U/mL DNase I for 45 min at 37°C. Cells were filtered through 70µm and 35µm strainers, centrifuged, and resuspended. Cell viability was assessed using AO/PI staining and Countstar Fluorescence Cell Analyzer. Single-cell RNA-seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3' V3.1 Reagent Kits. Cells were loaded to generate Gel Bead-In-Emulsions (GEMs). After reverse transcription, barcoded cDNA was amplified, fragmented, and ligated with adaptors. Library quality and quantity were assessed using the Qubit High Sensitivity DNA assay and Bioanalyzer 2200.
ENA first public
2026-02-18
INSDC center name
Department of Dermatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine
INSDC status
public
Submitter Id
E-MTAB-16629:Skin-H
age
30 year
broker name
ArrayExpress
cell type
mixed cell suspension
collection date
not collected
common name
human
developmental stage
adult
disease
Healthy skin
geographic location (country and/or sea)
not collected
individual
S1
isolate
not applicable
organism part
skin of prepuce of penis
sample name
E-MTAB-16629:Skin-H
scientific_name
Homo sapiens
sex
man