R7_mTAM_Junf-f
Mus musculus
Protocols: 300mg tumour fragments digested with tumour dissociation kit (Miltenyi BioTec) were stained with CD45.2-PE and CD45+ cell isolated with anti-PE beads (StemCell) after enrichment iTAM and mTAM populations were sorted by flow cytometry (10000 cells). Nucleic acid extraction protocol: Arcturus PicoPure. Sample prep (RNA-cDNA) \"Smart-Seq2\" - adapted from Picelli et al 2013 https://www.nature.com/articles/nmeth.2639, in-house method on robotics. The libraries for RNA-seq were prepared using ILL DNA Library Prep Tagmentation LV (Pur,FA) and quality of the RNA libraries was confirmed using fragment analyser
ENA first public
2026-05-24
INSDC center name
CRTD, TU Dresden
INSDC status
public
Submitter Id
E-MTAB-17032:R7_mTAM_Junf-f
age
10-12
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Junf/f
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Tumor associated cells
sample name
E-MTAB-17032:R7_mTAM_Junf-f
scientific_name
Mus musculus
sex
male
strain
C57BL6/J