KK1902_062_01_YN_pool
Cricetulus griseus
Chinese hamster lung (CHL)-YN cells (RIKEN Bioresource Cell Bank, RCB5004), serum-free suspension, 2 days post-seeding, antibody-producing, scRNA-Seq 76 cells analyzed
sample_name
KK1902_062_01_YN_pool
age
Passage 26, 2 days in culture
cell_line
CHL-YN
collection_date
2022-11-30
geo_loc_name
Japan:Osaka
growth_condition
Viable cell density: 2.76 x 10^6 cells/mL, Viability: 98.6%
growth_protocol
CHL-YN cells were cultured in 20 mL of EX-CELL CD CHO Fusion (MERCK, 14365C-1000M) supplemented with 600 uL of L-Glutamine (200 mmol/L stock; final concentration, 6.0 mM; FUJIFILM Wako, 073-05391) and 10 uL of Puromycin (10 mg/mL stock; final concentration, 5 ug/mL; InvivoGen, ant-pr-1) in a 125 mL flask. The flask was shaken (140 rpm) at 37 degree C in a humidified 5% CO2 atmosphere (80% relative humidity).
sex
not applicable
single_cell_identifier
KK1902_062_01_YN_pool
strain
CHL-YN
tissue
lung