CO-CL-YO-05
soil metagenome
clay loam from Yorkshire
ENA first public
2025-09-11
External Id
SAMEA114349213
INSDC center name
Scotland's Rural College - Aberdeen Campus
INSDC last update
2023-09-11T17:50:39Z
INSDC status
public
Submitter Id
ms00050
adapters
Forward overhang: 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG; Reverse overhang: 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG
broad-scale environmental context
environmental system determined by an organism
chimera check software
N/A
climate environment
controlled glasshouse
collection date
2021-12-30
environmental medium
bulk soil
experimental factor
bulk soil no plant control
geographic location (country and/or sea)
United Kingdom
geographic location (latitude)
51.8094
geographic location (longitude)
0.3561
host age
3
host common name
N/A
host life stage
N/A
host subspecific genetic lineage
N/A
host taxid
410658
local environmental context
soil environment
multiplex identifiers
N/A
negative control type
N/A
nucleic acid amplification
https://emea.support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.html
nucleic acid extraction
Qiagen(TM) Power soil gDNA extraction kit
pcr conditions
95�C for 3 minutes; 25 cycles of 95�C for 30 seconds, 55�C for 30 seconds, 72�C for 30 seconds; 72�C for 5 minutes; Hold at 4�C
pcr primers
Forward: 5' CCTACGGGNGGCWGCAG; Reverse: 5' GACTACHVGGGTATCTAATCC
plant body site
N/A
plant product
N/A
positive control type
N/A
project name
UK Crop Microbiome Cryobank
reference for biomaterial
Rothamsted Research Station
relevant electronic resources
agmicrobiomebase.org
sample name
ms00050
sample volume or weight for DNA extraction
0.5
scientific_name
soil metagenome
sequence quality check
software
sequencing method
MiSeq
target gene
16S