Sample 9
Drosophila melanogaster
Protocols: For each condition, 15 brains from wandering third instar larvae were dissected and pooled per biological replicate from 3 independent crosses. Brains were transferred into 1.5 ml tubes containing 250 µl peqGOLD TriFast (peqlab) and homogenised.  RNA was isolated according to manufacturer's instructions, digested with DNase with subsequent purification with acid phenol.  After integrity check with the Bioanalyzer RNA Pico Chips (Agilent), Illumina-compatible RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs).
ENA first public
2026-06-18
INSDC center name
University of Wuppertal Molecular Cell Biology and Microbiology
INSDC status
public
Submitter Id
E-MTAB-17059:Sample 9
age
larva
broker name
ArrayExpress
collection date
not collected
common name
fruit fly
developmental stage
third instar larva, wandering stage
genotype
tub-Gal80ts/UAS-mito-IMMDHFR; nSyb-Gal4/+
geographic location (country and/or sea)
not collected
individual
15 Individuals each
isolate
not applicable
organism part
whole brain
sample name
E-MTAB-17059:Sample 9
scientific_name
Drosophila melanogaster
sex
mixed
stimulus
Day3 after induction repl1
strain
tub-Gal80ts/UAS-mito-IMMDHFR; nSyb-Gal4/+