Sample 20
Homo sapiens
Protocols: CAR-NK-92, and NR3C1-knockout CAR-NK-92 cells were cultured under defined experimental conditions. Cells were treated with hydrocortisone and/or CoCl₂ and subsequently co-cultured with A549 target cells for 6 hours. Following co-culture, NK-92 cells were collected, washed in cold PBS, and pelleted in RNase-free tubes. Cell pellets were processed immediately for RNA extraction. Total RNA was extracted from sorted NK cells using the Qiagen RNA extraction kit following the manufacturer's instructions (including on-column DNase digestion when applicable). RNA quantity and purity were assessed using NanoDrop spectrophotometry. High-quality RNA (RIN ≥ 7) was used for downstream library preparation. RNA-seq libraries were sequenced by Novogene using an Illumina platform, generating paired-end reads. Standard Illumina sequencing chemistry and quality control procedures were applied. Raw FASTQ files were delivered after base calling, filtering, and adapter trimming performed by Novogene.
ENA first public
2025-12-19
INSDC center name
PhD student, Department of Pathology, University of Cambridge, UK
INSDC status
public
Submitter Id
E-MTAB-16367:Sample 20
broker name
ArrayExpress
cell line
NK-92
cell type
natural killer cell
collection date
not collected
common name
human
genetic deletion
NR3C1 KO
genetic modification
CEACAM5 specific CAR expression
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
blood
sample name
E-MTAB-16367:Sample 20
scientific_name
Homo sapiens
stimulus
Cocultured with CEACAM5 expressing A549 cell line