ACI 21
Arthrostemma ciliatum
Protocols: Multiple flower buds of a defined size (corresponding to the developmental stage) were collected from plants grown in the greenhouse.  Multiple flower buds corresponding to each developmental stage were dissected under a stereo microscope and the two stamen whorls (outer-antesepalous, inner-antepetalous) were separated into pre chilled, labelled Eppendorf tubes (kept in a metal rack on dry ice) for extraction.  Plants were grown in the public greenhouses at the Botanical Garden Munich Nymphenburg RNA was extracted from each stamen whorl following the manufacturers protocol using the RNeasy Plant Mini Kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
ENA first public
2026-06-18
INSDC center name
Ludwig-Maximilians-Universität München. Systematik, Biodiversität & Evolution der Pflanzen
INSDC status
public
Submitter Id
E-MTAB-16916:ACI 21
age
20 (from date of seed sowing)
broker name
ArrayExpress
collection date
not collected
developmental stage
flower development stage
developmental stage name
Stage-12
geographic location (country and/or sea)
not collected
isolate
not applicable
number of flowers
3
number of stamen
12
organism part
stamen
plant floral morph
Stamen dimorphic; Style short
sample name
E-MTAB-16916:ACI 21
scientific_name
Arthrostemma ciliatum
size
11-12
stamen whorl name
Antepetalous-inner
strain
Arthrostemma ciliatum Pav. ex D.Don