Jasp D2 V1
Caenorhabditis elegans
Protocols: After bleaching and L1 arrest, worms were grown at 22 °C for 72, 96, or 192 hours to obtain day 1, day 2, and day 6 adult animals, respectively. For each condition, approximately 1,000 worms were collected using M9 buffer and transferred into 1 mL of TRIzol Reagent for RNA extraction. After transfering to 1 mL of TRIzol Reagent, animals are subjected to three freeze/thaw cycles between liquid nitrogen and a 37°C bead bath, with 30 s vortexing between cycles. Following the final thaw, chloroform was added at a 1:5 ratio (chloroform:TRIzol), and RNA was separated by centrifugation in heavy gel phase‐lock tubes (VWR, 10847‐802). The aqueous phase was combined 1:1 with isopropanol, and purification was performed with a QuantaBio Extracta Plus RNA kit (95214) per the manufacturer's instructions. Library construction was performed by Novogene using their standard pipeline.
isolate
not applicable
organism
Caenorhabditis elegans
organism
Caenorhabditis elegans
collection date
not collected
sex
hermaphrodite
scientific_name
Caenorhabditis elegans
organism part
Whole organism
age
2
developmental stage
Adult
genotype
glp-4(bn2) I 6xBC
geographic location (country and/or sea)
not collected