Sample 2
Mus musculus
Protocols: Tumors were excised from LLC-OVA tumor–bearing C57BL/6J mice on day 19. Each tumor was mechanically minced and enzymatically digested using IMDM supplemented with 10% FBS, collagenase D (1 mg/mL), collagenase A (1 mg/mL), and DNase I (0.4 mg/mL). Digestion was performed at 37 °C for 30–40 minutes with gentle agitation. Collagenase activity was terminated by adding EDTA (5 mM) to the samples. The resulting suspension was filtered through 70 µm cell strainers to obtain a single-cell suspension. Tumor-infiltrating NK cells were isolated by flow cytometric sorting based on CD3⁻ CD19⁻ CD56⁺ immunophenotype and collected in RNase-free tubes for downstream processing. Total RNA was extracted from sorted NK cells using the Qiagen RNA extraction kit according to the manufacturer's instructions, including optional on-column DNase digestion. RNA concentration and purity were determined using a NanoDrop spectrophotometer, and RNA integrity was assessed using Bioanalyzer. Only high-quality RNA samples were used for library construction. RNA-seq libraries were prepared by Novogene using their standard mRNA-seq library preparation pipeline.
ENA first public
2025-12-19
INSDC center name
PhD Student, Department of Pathology, University of Cambridge, UK
INSDC status
public
Submitter Id
E-MTAB-16381:Sample 2
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Subcutaneous tumor
sample name
E-MTAB-16381:Sample 2
scientific_name
Mus musculus
strain
C57BL/6J