Secale cereale
ENA first public
2026-02-18
INSDC status
public
accession name
Lo7
accession_name
not collected
age
2-3 weeks
air_temperature_regimen
25°C day/18°C night
biological_material_altitude
112m
biological_material_coordinates_uncertainty
2000m
biological_material_doi
not collected
biological_material_geographic_location
Germany
biological_material_id
UL_seq_Rye_Lo7_1
biological_material_latitude
51.816
biological_material_longitude
11.283
biological_material_ploidy
diploid
checklist
ERC000011
collection date
not collected
genus
Secale
geographic location (country and/or sea)
Germany
humidity_regimen
~60% relative humidity
infraspecific_name
not collected
ipk_sequencing_date
2025-05-21
light_regimen
16 h light/8 h dark
material_source_altitude
not collected
material_source_description
not collected
material_source_doi
not collected
material_source_geographic_location
Germany
material_source_id
not collected
material_source_latitude
not collected
material_source_longitude
not collected
plant_anatomical_entity
leaf
plant_structure_development_stage
seedling
sample name
UL_seq_Rye_Lo7_1
sample_description
Plants were grown under controlled conditions in the greenhouse with a 16-hour light/8-hour dark photoperiod at 25°C (day) and 18°C (night). Humidity was maintained at 60%, and plants were irrigated with a nutrient solution weekly. Samples for DNA extraction were collected from young leaf tissues (2-3 weeks). High molecular weight DNA was isolated using the Macherey-Nagel HMW DNA kit. No fragment selection or additional shearing treatments were applied to maintain DNA integrity. The sequencing library was prepared with SQK-ULK114 chemistry and loaded onto a FLO-PRO114M Flow Cell (ID: PAO28231; run time = ~96 hours).
sample_id
IPK_LIMS:3400291
species
cereale