TN-No-DeMy-MKD-input-rep3
Rattus rattus
Protocols: Schwann tissues at REZ of different TN groups were harvested. Schwann cells are washed by PBS twice, and resuspended by 1 mL RIPA buffer, and put on ice for 30 min, followed by highest speed centrifugation for 15 min. The supernant containing with nucleus are transfered to another new tube. whole cell lysates are sonicated to break up the genomic DNA into 200-500 bp fragments. Then, 10% lysates were saved as input and the remaining were incubated with 1 μg IP grade antibody of SOX10 at 4oC overnight. This is followed by incubating for 2 h with Protein A beads (Thermo Fisher Scientific) at 37 °C to pull down the bound DNA fragments. DNA fragments from the pull-down is isolated by ethanol precipitation.For high throughput sequencing, we added 3'-dA overhang to the SOX10 enriched or input DNA and ligated them to the adapter to build a DNA library.
ENA first public
2026-05-22
INSDC center name
Suzhou Ninth People's Hospital
INSDC status
public
Submitter Id
E-MTAB-16991:TN-No-DeMy-MKD-input-rep3
age
12
broker name
ArrayExpress
collection date
not collected
common name
black rat
developmental stage
adult
genotype
myrf knockdown
geographic location (country and/or sea)
not collected
individual
TN-No-DeMy+myrf knockdown
isolate
not applicable
organism part
Myelin sheath
sample name
E-MTAB-16991:TN-No-DeMy-MKD-input-rep3
scientific_name
Rattus rattus
sex
male
strain
Sprague-Dawley