KK1904_263_09_ATF_d7
Cricetulus griseus
Chinese hamster lung (CHL)-YN cells (RIKEN Bioresource Cell Bank, RCB5004), serum-free suspension, 7 days post-seeding, antibody-producing, scRNA-Seq 77 cells analyzed
sample_name
KK1904_263_09_ATF_d7
age
Passage 151, 7 days in culture, on day 4 after the initiation of perfusion
cell_line
CHL-YN
collection_date
2023-01-20
geo_loc_name
Japan:Osaka
growth_condition
Viable cell density: 20.6 x 10^6 cells/mL, Viability: 96.7%
growth_protocol
CHL-YN cells were cultured in a 2 L animal cell culture bioreactor (BCP-02NP4, ABLE Corporation & Biott Corporation) operated under perfusion mode. The culture medium consisted of 900 mL of EX-CELL Advanced HD Perfusion Medium (MERCK, 24370C) supplemented with 67.5 mL of L-glutamine (200 mmol/L stock; final concentration, 15 mM; FUJIFILM Wako, 073-05391), 450 uL of puromycin (10 mg/mL stock; final concentration, 5.0 ug/mL; InvivoGen, ant-pr-1), and 0.9 mL of FoamAway Irradiated AOF (animal origin-free) Antifoaming Agent (final concentration, 0.1% (v/v); Gibco, A1036902). The culture was maintained at 37 degree C with shaking at 120 rpm, while dissolved oxygen (DO) was controlled at 3.43 ppm and pH at 7.2.
sex
not applicable
single_cell_identifier
KK1904_263_09_ATF_d7
strain
CHL-YN
tissue
lung