CQ1_S99
Mus musculus
Protocols: Male C57BL/6J mice (10 weeks old) received doxorubicin (5 or 10 mg/kg) by i.v. bolus injection on days 0 and 1 and were euthanized at 6, 24, 72, or 96 h after treatment initiation (Gall et al. 2023). Colon mucosa was collected for transcriptomic profiling following reported procedures; vehicle controls were handled in parallel. Total RNA was extracted using the miRNeasy Mini Kit (Qiagen). Total RNA was extracted using the miRNeasy Mini Kit (Qiagen) following the manufacturer's instructions. RNA quantity/purity were assessed by NanoDrop and RNA integrity was assessed by Agilent 2100 Bioanalyzer; samples with RIN > 6 and sufficient yield were used for library preparation. mRNA sequencing libraries were prepared from total RNA using the Lexogen SENSE mRNA-seq library preparation kit, following the manufacturer's instructions, including sample indexing for multiplexing.
ENA first public
2026-01-21
INSDC center name
Maastricht University, Department of Translational Genomics (GROW)
INSDC status
public
Submitter Id
E-MTAB-16565:CQ1_S99
age
10
broker name
ArrayExpress
collection date
not collected
common name
house mouse
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
colonic mucosa
response to treatment
DOX
sample name
E-MTAB-16565:CQ1_S99
scientific_name
Mus musculus
sex
male
strain
C57BL/6J