KK1903_139_05_S_d5
Cricetulus griseus
Chinese hamster ovary (CHO-S) cells (FreeStyle CHO-S Cells, Thermo Fisher Scientific, R80007), serum-free suspension, 5 days post-seeding, scRNA-Seq 88 cells analyzed
sample_name
KK1903_139_05_S_d5
age
Passage 20, 5 days in culture
cell_line
CHO-S
collection_date
2022-12-21
geo_loc_name
Japan:Osaka
growth_condition
Viable cell density: 5.74 x 10^6 cells/mL, Viability: 96.3%
growth_protocol
CHO-S cells were cultured in 20 mL of FreeStyle CHO Expression Medium (Gibco, 12651-014) supplemented with 600 uL of L-Glutamine (200 mmol/L stock; final concentration, 6.0 mM; FUJIFILM Wako, 073-05391) in a 125 mL flask. The flask was shaken (140 rpm) at 37 degree C in a humidified 5% CO2 atmosphere (80% relative humidity).
sex
not applicable
single_cell_identifier
KK1903_139_05_S_d5
strain
CHO-S
tissue
ovary