C41_UDI_56_S98
Mus musculus
Protocols: After exposure (24/48/72 h), Matrigel domes were dissolved on ice with cold PBS and colonoids were pelleted by centrifugation (200 × g, 5–10 min, 4 °C). Pellets were transferred to RNAlater (4 °C overnight) and stored at −80 °C until RNA extraction. Total RNA was extracted using the miRNeasy Mini Kit (Qiagen) following the manufacturer's instructions. RNA quantity/purity were assessed by NanoDrop and RNA integrity was assessed by Agilent 2100 Bioanalyzer; samples with RIN > 6 and sufficient yield were used for library preparation. mRNA sequencing libraries were prepared from total RNA using the Lexogen SENSE mRNA-seq library preparation kit, following the manufacturer's instructions, including sample indexing for multiplexing.
ENA first public
2026-01-21
INSDC center name
Maastricht University, Department of Translational Genomics (GROW)
INSDC status
public
Submitter Id
E-MTAB-16537:C41_UDI_56_S98
broker name
ArrayExpress
collection date
not collected
common name
house mouse
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
colonic mucosa
response to treatment
DOX
sample name
E-MTAB-16537:C41_UDI_56_S98
scientific_name
Mus musculus
strain
C57BL/6J