VKORC1_2
Homo sapiens
Protocols: Human coronary artery endothelial cells (HCAEC) were cultured under standard conditions and harvested at 24h point following siRNA transfection. Cells were washed with PBS and collected directly into lysis reagent (Trizol)for RNA isolation. Total RNA was extracted using TRIzol–chloroform phase separation followed by isopropanol precipitation and ethanol washes. RNA was resuspended in RNase-free water and assessed for concentration, purity, and integrity prior to library preparation. RNA-seq libraries were prepared from total RNA using poly(A) mRNA enrichment and strand-specific library preparation. mRNA was fragmented, reverse transcribed, end-repaired, adapter-ligated, PCR-amplified, and quality-checked before sequencing.
ENA first public
2026-02-18
INSDC center name
Universitätsklinikum Bonn, Medizinische Klinik II - Herzzentrum
INSDC status
public
Submitter Id
E-MTAB-16661:VKORC1_2
broker name
ArrayExpress
cell type
endothelial cell
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
coronary artery
sample name
E-MTAB-16661:VKORC1_2
scientific_name
Homo sapiens
stimulus
vkorc1 siRNA