LatA D6 V2
Caenorhabditis elegans
Protocols: After bleaching and L1 arrest, worms were grown at 22 °C for 72, 96, or 192 hours to obtain day 1, day 2, and day 6 adult animals, respectively. For each condition, approximately 1,000 worms were collected using M9 buffer and transferred into 1 mL of TRIzol Reagent for RNA extraction. After transfering to 1 mL of TRIzol Reagent, animals are subjected to three freeze/thaw cycles between liquid nitrogen and a 37°C bead bath, with 30 s vortexing between cycles. Following the final thaw, chloroform was added at a 1:5 ratio (chloroform:TRIzol), and RNA was separated by centrifugation in heavy gel phase‐lock tubes (VWR, 10847‐802). The aqueous phase was combined 1:1 with isopropanol, and purification was performed with a QuantaBio Extracta Plus RNA kit (95214) per the manufacturer's instructions. Library construction was performed by Novogene using their standard pipeline.
ENA first public
2026-05-11
INSDC center name
University of Southern California Leonard Davis School of Gerontology
INSDC status
public
Submitter Id
E-MTAB-16193:LatA D6 V2
age
6
broker name
ArrayExpress
collection date
not collected
developmental stage
Adult
genotype
glp-4(bn2) I 6xBC
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Whole organism
sample name
E-MTAB-16193:LatA D6 V2
scientific_name
Caenorhabditis elegans
sex
hermaphrodite