Homo sapiens
scDNA Seq data from a custom Mission BioTapestri amplicon panel, designed to capture 56 potential ABE target regions across the human genome including the 20 distinct target genes that have been edited in these samples (CD3E, B2M, CIITA, PVR, CD52, ICAM1, CD58, PDCD1, CTLA4, CD5, RASA2, MED12, ZC3H12A, RC3H1, TET2, ARID1A, SMARCC1, SMARCA4, SMYD3, ADORA2A).
isolate
Healthy human donor
age
34 years
biomaterial_provider
Carl H. June, Perelman School of Medicine, University of Pennsylvania, Smilow Center for Translational Research, 3400 Civic Center Boulevard, Building 421, 8th Floor, Room 123, Philadelphia, PA. 19104-5156
collection_date
2024-08-13
geo_loc_name
USA: Philadelphia, Pennsylvania
sex
male
tissue
Primary human T cells
treatment
Multiplex adenine base editing of 20 distinct genes (CD3E, B2M, CIITA, PVR, CD52, ICAM1, CD58, PDCD1, CTLA4, CD5, RASA2, MED12, ZC3H12A, RC3H1, TET2, ARID1A, SMARCC1, SMARCA4, SMYD3, ADORA2A) using a high dose of gRNAs (5µg per gRNA).
BioSampleModel
Human