Cas9 SL1 targeting Extracellular Vesicles
Homo sapiens
Amplicon NGS
isolate
DNA from HEK293T cells transfected with extracellular vesicles containing Cas9 and a sgRNA targeting a fluorescent Stoplight reporter construct
age
passage 53
biomaterial_provider
Olivier de Jong, Pharmaceutics, UU, Universiteitsweg 99, 3584 CG, Utrecht, The Netherlands
collection_date
2025-03-04
geo_loc_name
Netherlands:Utrecht
sex
female
tissue
Cell line
cell_line
HEK293T
culture_collection
ATCC:3216
sample_type
Cell culture
treatment
Cas9 extracellular vesicle delivery
Barcoded primers ON-target Stoplight sequence
replicate 1: tcacgctgtacaaggtgaagcagacc, replicate 2: gaccactgtacaaggtgaagcagacc, replicate 3: ccaatctgtacaaggtgaagcagacc
Barcoded primers Off-target 1 (chr2:+120580702)
replicate: 1 tcacgCTCTTGCTGACTCGACTGGT, replicate 2: gaccaCTCTTGCTGACTCGACTGGT, replicate 3: ccaatCTCTTGCTGACTCGACTGGT
Barcoded primers Off-target 2 (chr4:157825214)
replicate 1: tcacgGTGCAGGGAAAACACCATAGG, replicate 2: gaccaGTGCAGGGAAAACACCATAGG, replicate 3: ccaatGTGCAGGGAAAACACCATAGG
Barcoded primers Off-target 3 (chr14:104942109)
replicate 1: tcacgGGCATCTTGAACTTGGGCAT, replicate 2: gaccaGGCATCTTGAACTTGGGCAT, replicate 3: ccaatGGCATCTTGAACTTGGGCAT
Barcoded primers Off-target 4 (chr5:180630776)
replicate 1: tcacgCTCGCAGTCTCGCACCAC, replicate 2: gaccaCTCGCAGTCTCGCACCAC, replicate 3: ccaatCTCGCAGTCTCGCACCAC
Barcoded primers Off-target 5 (chr8:135581932)
replicate 1: tcacgTGACTCTCTTGGTTACAGGGG, replicate 2: gaccaTGACTCTCTTGGTTACAGGGG, replicate 3: ccaatTGACTCTCTTGGTTACAGGGG
BioSampleModel
Human