The right-hand side of the longitudinally halved roots before gravistimulation
Eight seeds of Cucumis sativus L. cv. Shinkasyuu-jibai (Watanabe Seed Co., Kogota, Miyagi, Japan) were vertically placed in a crack within a block of water-absorbent plastic foam (45 mm x 20 mm x 10 mm). The two blocks with cucumber seeds were attached onto the inner surface of a plastic cap and sealed in a plastic container (60 mm x 60 mm x 60 mm) so that seedling roots could be aeroponically grown in humid air after germination. Thus, seeds were incubated in a vertical position for 24 h at 26 degree Celsius after imbibition in the container. The resulting seedlings were put into RNAlater and stored at 4 degree Celsius overnight. Then, 0.5 mm of root-tip was removed, and 1.5 mm remaining root proper was longitudinally cut into halves between the right- and left-hand sides under a microscope. This sample was the right-hand side of the longitudinally halved roots.