Water-absorbent plastic side (wet side) of longitudinally halved roots from cucumber seedlings germinated under stationary conditions and exposed to H2O saturation
Cucumber seeds were germinated as described for sample A1. After the initial 18 h germination phase, seedlings were exposed to H2O-saturated conditions and grown for a further 4 h under stationary conditions. Seedlings were then placed in RNAlater and stored at 4 degree Celsius overnight. A small section of root tip (0.5 mm) was removed, and a 1.5 mm section of the remaining root was divided longitudinally into the water-absorbent plastic (wet) side (this sample) and the opposite (dry) side (sample A6).