The upper side of the roots longitudinally halved after exposing cucumber seedlings to gravistimulation for 1 h
Cucumber seeds were germinated as the sample 'y1' and 'y2', and the 24-h-old seedlings were further grown for 1 h in a horizontal position. Then, the seedlings was put into RNAlater and stored at 4 degree Celsius overnight. The root-tip in 0.5 mm length was removed, and remaining 1.5 mm root proper was longitudinally cut into halves of the upper and lower sides under a microscope. This sample was the upper side of the longitudinally halved roots.