mouse_WT5
Protocols: Naked Mole rat: All experimental procedures followed federal and institutional guidelines and were approved by the Magee-Womens Research Institute, University of Pittsburgh (IACUC protocol# IS00024112). Naked mole-rat colonies were housed in polycarbonate cages of three sizes (large: 65 cm L × 45 cm W × 23 cm H; medium: 46 cm L × 24 cm W × 15 cm H; small: 30 cm L × 18 cm W × 13 cm H) connected by tubes (25 cm L × 18 cm D) and lined with corn cob bedding. Naked mole-rats were kept on a 12:12 light/dark cycle at 28–30 °C, humidity 40-55%, and fed ad libitum with a diet consisting of sweet potato, grapes, celery, apples, corn, bananas, yams, and Pronutro (Bokomo, South Africa). Ex-subordinate males were coupled with a single female during a year; NMR were euthanized using isoflurane administration, followed by cervical dislocation.  Mouse: All mouse studies were conducted with prior approval from the Magee-Womens Research Institute and the University of Pittsburgh Institutional Animal Care and Use Committee (Protocol # IS00020751). The mice were bred under pathogen-free, controlled temperature (22±1°C) and regulated humidity (50–55%) conditions, with 12 h light-dark cycles and food available ad libitum. Mice were euthanized using CO2 administration, followed by cervical dislocation.  Total RNA was extracted from each tissue sample using the NucleoSpin miRNA Kit (Macherey-Nagel) according to the manufacturer's instructions. Tissue samples were lysed in NucleoZOL (Macherey-Nagel) and transferred to bead mill tubes containing six 2.8 mm ceramic beads (Fisher Scientific, Cat. No. 19-628-3). Samples were homogenized using an Omni Bead Ruptor 24 bead mill at 5m/sec for one cycle of 30 seconds seconds at 4°C. RNA concentration and purity were assessed using a NanoDrop spectrophotometer, and RNA integrity was evaluated using an Agilent 2100 Bioanalyzer.  The E3330S- Nebnext ultra express RNA library prep kit was used following the manufacturers protocol.
ENA first public
2026-07-31
INSDC center name
The Hospital for Sick Children, Toronto Ontario, Canada The Ontario Institute for Cancer Research, Toronto, Ontario, Canada
INSDC status
public
Submitter Id
E-MTAB-17270:mouse_WT5
age
8
broker name
ArrayExpress
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
WT5
isolate
not applicable
organism part
sperm from epididymis
sample name
E-MTAB-17270:mouse_WT5
scientific_name
Mus musculus
sex
male
strain
C57BL6J