Sample 2
Protocols: Wild-type C57BL/6J mice (6–8 weeks old) were sensitised intranasally under light isoflurane anaesthesia with 1 µg house dust mite (HDM) extract in 40 µL PBS, followed seven days later by intranasal challenges of 10 µg HDM once daily for five consecutive days. Four days after the final challenge (day 15), mice were euthanised and BAL was collected by two sequential flushes of the airways with 1 mL PBS and , medLN, lung tissue were collected at the same time. Cells were stained for surface markers and labelled with TotalSeq-C hashtag antibodies (BioLegend) for sample identification. CD3⁺CD4⁺ T cells were sorted into FCS on a BD FACSAria Fusion, washed, and resuspended in cold PBS for single-cell capture. No bulk nucleic acid extraction was performed.  Sorted CD3⁺CD4⁺ T cells in cold PBS were loaded onto the 10x Genomics Chromium controller for droplet partitioning with barcoded gel beads. Cell capture was performed by the National Genomics Infrastructure (NGI), SciLifeLab, Stockholm. The full-length cDNA was used to generate three libraries per reaction: (i) a 5′ gene-expression library, (ii) a paired T-cell receptor (TCR) V(D)J libraryand (iii) a cell-hashing library used for sample demultiplexing. Libraries were prepared with the 10x Genomics Chromium Single Cell 5′ v1 Immune Profiling reagent kit. Library preparation was performed by NGI, SciLifeLab, Stockholm.
ENA first public
2026-07-31
INSDC center name
KIS
INSDC status
public
Submitter Id
E-MTAB-17402:Sample 2
age
6–8
broker name
ArrayExpress
cell type
CD4+ CD3+ B220−
collection date
not collected
common name
house mouse
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Bronchoalveolar lavage, lung tissue and mediastinal lymph nodes
sample name
E-MTAB-17402:Sample 2
scientific_name
Mus musculus
stimulus
house dust mite (HDM) extract
strain
C57BL/6J