E8832
Protocols: The nematodes were propagated on tomato cv. Money Maker. Fresh eggs were harvested 10 weeks after the nematodes were inoculated. Eggs were obtained by treating roots with 0.05% (v/v) NaOCl for three minutes, following by rinsing with tap water. After further rinsing, eggs were hatched on a 25 µm sieve. Hatched J2 were collected by centrifugation on a 70% sucrose column. The purified J2s were lysed with nematode lysis buffer with proteinase-K and beta-mercaptoethanol. This was done in a ThermoCycler at 60 ºC for 1 h with continuous shaking at 650 RPM. The lysate was then mixed with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) (pH 8.0) following a standard DNA purification procedure, followed by precipitation with isopropanol. Afterwards, the DNA pellet was washed with 70% ethanol for two times, before finally resuspended with 10 mM Tris-HCL (pH 8.0). DNA library preparation and sequencing was done in Utrecht sequencing facility (USEQ). Library preparation was done using the Illumina TruSeq protocol.
ENA first public
2026-07-31
INSDC center name
Wageningen University and Research
INSDC status
public
Submitter Id
E-MTAB-17107:E8832
broker name
ArrayExpress
collection date
not collected
developmental stage
J2
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
whole organism
sample name
E-MTAB-17107:E8832
scientific_name
Meloidogyne hapla
strain
E8832