FKBP12(F36V)-ZNF24_clone2_DMSO
Protocols: 1 million HAP1 cells were detached by incubating in trypsin for 2 min. HAP1 cells (Horizon Discovery, C631) were cultured in IMDM (Thermo Fisher, 12440061) supplemented with 10% foetal calf serum (Merck, F9665-500ML, Lot #19A124) and 1X Penicillin-Streptomycin-L-Glutamine (Corning, 30-009-CI).  To induce the degradation of ZNF24 endogenously tagged with an FKBP12(F36V), the cells were cultured in medium containing either 500 nM dTAGv-1 (MedChemExpress, HY-145514) or the same volume of DMSO for 72 h prior to harvest. RNA was extracted with High Pure RNA Isolation Kit (Roche, 11828665001). 500 ng of the isolated RNA was used to prepare RNA-seq libraries using QuantSeq 3′ mRNA-Seq V2 Library Prep Kit (Lexogen, 191.24) following the manufacturer's instruction.
isolate
not applicable
organism
Homo sapiens
organism
Homo sapiens
cell_line
HAP-1
collection date
not collected
scientific_name
Homo sapiens
common name
human
geographic location (country and/or sea)
not collected