Sample 25
Protocols: CD8+ untransduced T cells, CD176-iEGFP-, CD176-iIL18-, or CD176-iIL12-TRUCKs from 4 different donors, before or after 48 h co-cultures with A549 cells at a 1:1 ratio, and A549 cells cultured alone for 48 h were used for bulk RNA analysis. Suspension cells from each sample were resuspended Total RNA was isolated using RNeasy Plus Kit (QIAGEN, Hilden, Germany) according to manufacturer's instructions. The RNA sequencing library was generated from 300 ng total RNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module, followed by the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (both from New England Biolabs®, Ipswich, MA, USA) according to the manufacturer's protocols, except that all reactions were downscaled to 2/3 of initial volumes.
ENA first public
2026-07-31
INSDC center name
IIT
INSDC status
public
Submitter Id
E-MTAB-17389:Sample 25
broker name
ArrayExpress
collection date
not collected
common name
human
geographic location (country and/or sea)
not collected
individual
4
isolate
not applicable
organism part
mixed
sample name
E-MTAB-17389:Sample 25
scientific_name
Homo sapiens