Sample 32
Protocols: Total blood was collected in BD Vacutainer tubes (BD-Becton Dickinson and Company, NJ) with ethylenediaminetetraacetic acid as clot activator, and stored at -20C DNA extracted from total blood using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, USA) and quantified by fluorimetry using the Picogreen dsDNA Quantitation Kit (Life Technologies)  Ion AmpliSeqâ„¢ EXome RDY Library Preparation kit from Ion Torrent was used to generate indexed libraries from each sample DNA. Starting from 100ng genomic DNA, exonic target regions were amplified using a multiplex PCR approach; barcoded adapters were ligated to amplicons for each sample. Barcoded exome libraries were QC, equalized and combined for sequencing.
disease
non-small cell lung adenocarcinoma
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
female
scientific_name
Homo sapiens
common name
human
organism part
blood
developmental stage
adult
isolate
not applicable
age
41
geographic location (country and/or sea)
not collected