Sample 2
Protocols: Cells underwent neural induction of 7 days with chemically defined medium containing 1μM Dorsomorphin (Millipore), 2μM SB431542 (Tocris Bioscience) and 3.3μM CHIR99021 (Miltenyi Biotec), patterning with 0.5μM retinoic acid and 1μM purmorphamine for 7 days with a 4 day expansion in 0.1μM purmorphamine and finally plated out for terminal differentiation in the presence of compound E iPSCs were maintained on Matrigel (Corning Life Sciences) with Essential 8 Medium media (Life technologies), cells were passaged using EDTA (Life technologies, 0.5mM).  Cells underwent neural induction of 7 days with chemically defined medium containing 1μM Dorsomorphin (Millipore), 2μM SB431542 (Tocris Bioscience) and 3.3μM CHIR99021 (Miltenyi Biotec), patterning with 0.5μM retinoic acid and 1μM purmorphamine for 7 days with a 4 day expansion in 0.1μM purmorphamine and finally plated out for terminal differentiation in the presence of compound E RNA extraction was performed using the Promega Maxwell RSC simplyRNA kit (including DNAase treatment) and the Maxwell RSC instrument mRNA-Seq libraries were prepared using the polyA_KAPA_mRNA_Hyper_Prep kit (Roche, UK)
strain
SBMA
individual
WT_3m
organism
Mus musculus
organism
Mus musculus
collection date
not collected
disease staging
Presymptomatic
sex
male
scientific_name
Mus musculus
cell type
motor neuron
common name
house mouse
organism part
Spinal cord motor neurons
developmental stage
adult
genotype
wild type genotype
isolate
not applicable
genetic modification
WT
age
3 months
geographic location (country and/or sea)
not collected