PEO4_MI_rep1
Protocols: PEO1 (ECACC 10032308), PEO4 (ECACC 10032309), PEO14 (ECACC 10032311) were purchased from the American European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK), whereas ovarian cancer cell lines OVCAR-3 (HTB-161) and Caov-3 (HTB-75) were ordered from American Type Culture Collection (ATCC, Manassas, VA, USA) Cells were cultivated according to manufacturers' instructions at 37°C and in the presence of 5%CO 2 . ATCC formulated RPMI 1640 medium (Euroclone, Milan, Italy), supplemented with 10%FBS (GE Healthcare, Chicago, IL) was used for maintenance of PEO1, PEO4, and PEO14 celllines. RPMI 1640 medium supplemented with 20% FBS and 0.01 mg/ml bovine insulin (Merck,Darmstadt, Germany) was used for cultivation of OVCAR-3 cell. Caov-3 cells were grown inDMEM (Euroclone) supplemented with 10% FBS. For all cell lines, 100 U/ml penicillin (Lonza,Basel, Swizzerland), 100 mg/mL streptomycin (Lonza), and 250 ng/mL Amphotericin-B (Merck)were added to the culture medium. Mycoplasma contamination of cells was routinely tested using amycoplasma PCR detection kit (ABM, Richmond, BC, Canada). For each sample, 1 µg of total RNA were used for rRNA depletion by using MGIEasy rRNA Depletion Kit (MGI). Then, the purified RNA was used as input for sequencing single-stranded circular DNA libraries preparation with MGIEasy RNA Directional Library Prep Set (MGI). Finally, according to the manufacturer's' instructions of DNBSEQ-G50RS High throughput Sequencing Kit (FCL PE100/FCS PE150) (MGI), single-stranded DNA libraries were used to make DNA nanoballs (DNBs)
isolate
not applicable
organism
Homo sapiens
organism
Homo sapiens
growth condition
MI
collection date
not collected
scientific_name
Homo sapiens
common name
human
organism part
ovary
cell line
PEO4
geographic location (country and/or sea)
not collected