Sample 2_hash
Protocols: Mesenteric white adipose tissue from wildtype C57BL/6 and Wipf1-/- mice was harvested through vivosection, minced and digested. Tissue-resident lymphocytes were enriched with Percoll gradient centrifugation. CD45 staining and fluorescence associated cell sorting were performed to achieve a pure lymphocyte population.Single cell suspension was made with 20.000 of these cells on the 10x genomics platform and following the Chromium Next GEM Single Cell 3' Reagent Kit User Guide Revision E. Nucleic acid extraction was performed following the Chromium Next GEM Single Cell 3' Reagent Kit User Guide Revision E.  Library preparation was performed following the Chromium Next GEM Single Cell 3' Reagent Kit User Guide Revision E.
ENA first public
2026-07-31
INSDC center name
Technische Universität München
INSDC status
public
Submitter Id
E-MTAB-17384:Sample 2_hash
age
13
age unit
weeks
broker name
ArrayExpress
cell type
CD45 positive lymphocytes
collection date
not collected
common name
house mouse
developmental stage
adult
disease
autoimmune disease
genotype
Wipf1-/-
geographic location (country and/or sea)
not collected
individual
4-5
isolate
not applicable
organism part
gonadal and mesenteric adipose tissue
sample name
E-MTAB-17384:Sample 2_hash
scientific_name
Mus musculus
sex
female
strain
C57BL/6