Sample 5
Protocols: Total blood was collected in BD Vacutainer tubes (BD-Becton Dickinson and Company, NJ) with ethylenediaminetetraacetic acid as clot activator, and stored at -20C DNA extracted from total blood using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, USA) and quantified by fluorimetry using the Picogreen dsDNA Quantitation Kit (Life Technologies)  Indexed fragment libraries were prepared from 150 ng of genomic DNA using Illumina DNA Prep (S) Tagmentation kit and processed with xGen Exome Research Panel v2 (IDT) for enrichment of exonic probes to generate final WES libraries. Exonic target regions were amplified using a multiplex PCR approach. Barcoded exome libraries were QC, equalized and combined for sequencing.
isolate
not applicable
disease
non-small cell lung carcinoma
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
female
scientific_name
Homo sapiens
common name
human
organism part
blood
age
46
geographic location (country and/or sea)
not collected