WT_2
Protocols: Fresh mouse testes were dissected, with adherent adipose tissue carefully removed, washed twice in PBS, flash-frozen in liquid nitrogen, and stored for subsequent RNA extraction. Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions. The transcriptome sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China).
strain
C57/B6J
organism
Mus musculus
organism
Mus musculus
collection date
not collected
sex
male
scientific_name
Mus musculus
common name
house mouse
organism part
testis
genotype
wild type genotype
isolate
not applicable
age
Postnatal day 10 (P10)
geographic location (country and/or sea)
not collected