E10_23GFP_ATAC
Protocols: AVU from pooled E9 (33 embryos, 20-25 sp) and E10 (24 embryos, 32-36 sp) 23GFP Tg were dissected, processed into single cell suspension and stained for FACS sorting, as described above. Ter119-23GFP+ cells were FACS-sorted using a BD FACSAria Fusion sorter and nuclei were isolated as 10x Genomics demonstrated protocols (CG000365). Nuclei quality was assessed, and nuclei were counted before loading each sample onto independent channels of a Chromium chip for single cell partitioning and barcoding on Chromium Controller (10x Genomics). Nucleic acid extraction was performed according to manufacturer's  guidelines for Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Kit (10x Genomics). Gene expression (GEX) and chromatin accessibility (ATAC) library preparation were performed using a Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Kit (10x Genomics) as per manufacturer's instructions.
strain
(CBA x C57BL/6)F1
organism
Mus musculus
organism
Mus musculus
collection date
not collected
scientific_name
Mus musculus
cell type
embryonic cell
common name
house mouse
organism part
aorta-gonad-meseonephros + vitelline and umbilical arteries
developmental stage
embryo stage
genotype
23GFP Tg
isolate
not applicable
age
E10
geographic location (country and/or sea)
not collected