WT
Protocols: E10.5 embryonic hearts was dissected and quickly frozen in liquid nitrogen to retain intact nuclei.  After nuclei extraction and purification, approximately 15,000 nuclei per sample were loaded on a Chromium Controller (10x Genomics) to generate gel bead-in-emulsions.  The sc-RNA-seq libraries were generated using Chromium Single Cell 3' Reagent Kits v2 and quantified using a Bioanalyzer 2200.
ENA first public
2026-07-31
INSDC center name
SINH,CAS
INSDC status
public
Submitter Id
E-MTAB-14376:WT
broker name
ArrayExpress
cell_line
embryonic cell line
cell_type
endothelial cell of coronary artery
collection date
not collected
common name
house mouse
developmental stage
mouse embryo stage
disease
coronary heart disease
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
heart
sample name
E-MTAB-14376:WT
scientific_name
Mus musculus