rgef-1p::bet-1B x glp-4 V1
Protocols: For all day 1 RNA-seq experiments, after bleaching and L1 arresting, worms were grown at 22°C for 72 hours (i.e., 3 days) to obtain day 1 adult animals. For day 7 RNA-seq experiments, we used N2 worms, as animals no longer have germline at day 7 of adulthood. After bleaching and L1 arresting, animals were grown at 20 °C for 72 hours (i.e., 3 days). Animals were then transferred to FUDR and grown for an additional 6 days prior to sample collection. Roughly 1,000 worms per condition were harvested with M9, transferred to 1 mL of TRIzol Reagent.  After transfering to 1 mL of TRIzol Reagent, animals are subjected to three freeze/thaw cycles between liquid nitrogen and a 37°C bead bath, with 30 s vortexing between cycles. Following the final thaw, chloroform was added at a 1:5 ratio (chloroform:TRIzol), and RNA was separated by centrifugation in heavy gel phase‐lock tubes (VWR, 10847‐802). The aqueous phase was combined 1:1 with isopropanol, and purification was performed with a QuantaBio Extracta Plus RNA kit (95214) per the manufacturer's instructions. Library construction was performed by Novogene using their standard pipeline.
ENA first public
2026-07-31
INSDC center name
University of Southern California Leonard Davis School of Gerontology
INSDC status
public
Submitter Id
E-MTAB-15906:rgef-1p::bet-1B x glp-4 V1
age
1
broker name
ArrayExpress
collection date
not collected
developmental stage
adult
genotype
sybIs4006[rgef-1p::bet-1b, myo-2p::mCherry] 8xBC; glp-4(bn2) I 6xBC;
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
Whole organism
sample name
E-MTAB-15906:rgef-1p::bet-1B x glp-4 V1
scientific_name
Caenorhabditis elegans
sex
hermaphrodite