E10_23GFP_CS
Protocols: AGM+VU from pooled E10 23GFP Tg (9 embryos, 32-35 sp) and 110GFP Tg (5 embryos, 32-34 sp) were dissected and processed into single cell suspension. Cells were incubated in TruStain FcX™ PLUS blocking reagent (Biolegend) and TotalSeqB antibody cocktail (Biolegend), as per manufacturer's instructions. 23GFP+ and 110GFP+ cells were FACS-sorted using a BD FACSAria Fusion sorter and cell viability was assessed (99% and 95% live cells for 23GFP and 110GFP, respectively) before loading each sample onto independent channels of a Chromium chip for single cell partitioning and barcoding on Chromium Controller.  Nucleic acid extraction was performed according to manufacturer's  guidelines for Chromium Next GEM Single Cell 3' v3.1 Dual Index Kit (10x Genomics). Gene expression (GEX) libraries were prepared using a Chromium Next GEM Single Cell 3' v3.1 Dual Index Kit (10x Genomics) as per manufacturer's instructions. Cell Surface (CS) libraries (TotalSeq-B, Biolegend) were prepared as per Chromium Next GEM Single Cell 3ʹReagent Kits v3.1 with Feature Barcoding technology for Cell Surface Protein user guide.
strain
(CBA x C57BL/6)F1
organism
Mus musculus
organism
Mus musculus
collection date
not collected
scientific_name
Mus musculus
cell type
embryonic cell
common name
house mouse
organism part
aorta-gonad-meseonephros + vitelline and umbilical arteries
developmental stage
embryo stage
genotype
23GFP Tg
isolate
not applicable
age
E10
geographic location (country and/or sea)
not collected