KO
Protocols: E10.5 embryonic hearts was dissected and quickly frozen in liquid nitrogen to retain intact nuclei.  After nuclei extraction and purification, approximately 15,000 nuclei per sample were loaded on a Chromium Controller (10x Genomics) to generate gel bead-in-emulsions.  The sc-RNA-seq libraries were generated using Chromium Single Cell 3' Reagent Kits v2 and quantified using a Bioanalyzer 2200.
ENA first public
2026-07-31
INSDC center name
SINH,CAS
INSDC status
public
Submitter Id
E-MTAB-14376:KO
broker name
ArrayExpress
cell_line
embryonic cell line
cell_type
endothelial cell of coronary artery
collection date
not collected
common name
house mouse
developmental stage
mouse embryo stage
disease
coronary heart disease
genotype
Emp2-/-
geographic location (country and/or sea)
not collected
isolate
not applicable
organism part
heart
sample name
E-MTAB-14376:KO
scientific_name
Mus musculus