Sample 30
Protocols: Cells underwent neural induction of 7 days with chemically defined medium containing 1μM Dorsomorphin (Millipore), 2μM SB431542 (Tocris Bioscience) and 3.3μM CHIR99021 (Miltenyi Biotec), patterning with 0.5μM retinoic acid and 1μM purmorphamine for 7 days with a 4 day expansion in 0.1μM purmorphamine and finally plated out for terminal differentiation in the presence of compound E iPSCs were maintained on Matrigel (Corning Life Sciences) with Essential 8 Medium media (Life technologies), cells were passaged using EDTA (Life technologies, 0.5mM).  Cells underwent neural induction of 7 days with chemically defined medium containing 1μM Dorsomorphin (Millipore), 2μM SB431542 (Tocris Bioscience) and 3.3μM CHIR99021 (Miltenyi Biotec), patterning with 0.5μM retinoic acid and 1μM purmorphamine for 7 days with a 4 day expansion in 0.1μM purmorphamine and finally plated out for terminal differentiation in the presence of compound E RNA extraction was performed using the Promega Maxwell RSC simplyRNA kit (including DNAase treatment) and the Maxwell RSC instrument mRNA-Seq libraries were prepared using the polyA_KAPA_mRNA_Hyper_Prep kit (Roche, UK)
strain
SBMA
individual
AR20_18m
organism
Mus musculus
organism
Mus musculus
collection date
not collected
disease staging
Late Stage of Disease
sex
male
scientific_name
Mus musculus
cell type
motor neuron
common name
house mouse
organism part
Spinal cord motor neurons
developmental stage
adult
genotype
AR100 pathogenic
isolate
not applicable
genetic modification
AR100: 20 CAG Repaets
age
18 months
geographic location (country and/or sea)
not collected