Shard 11
Protocols: Fresh saliva was obtained from a healthy (non-patient) human donor (ethical permit # 08-047M). The saliva (5mL) was collected in a 50mL falcon tube and vortexed for 3 min with glass beads (Fisherbrand, catalog #41401001). The sample was subsequently filtered using a 35μm filter (Cat no. 352235, Corning). The filtrate was further centrifuged at 5,000g for 10 minutes. The supernatant was discarded and the pellet was washed with 1mL of PBS three times. Finally, the pellet was resuspended in 10% glycerol and stored at -80°C. Nucleic acid was extracted according to lysis protocols R1, R2, R3 described further in our manuscript Libraries were prepared using SPCs as specified in our manuscript
ENA first public
2026-07-31
INSDC center name
Department of molecular biology Umeå university
INSDC status
public
Submitter Id
E-MTAB-15285:Shard 11
broker name
ArrayExpress
collection date
not collected
common name
human
disease
normal
genotype
wild type genotype
geographic location (country and/or sea)
not collected
isolate
not applicable
organism substance
saliva
sample name
E-MTAB-15285:Shard 11
sample type
human oral metagenome
scientific_name
Homo sapiens
shard
12