Sample 39
Protocols: Cells underwent neural induction of 7 days with chemically defined medium containing 1μM Dorsomorphin (Millipore), 2μM SB431542 (Tocris Bioscience) and 3.3μM CHIR99021 (Miltenyi Biotec), patterning with 0.5μM retinoic acid and 1μM purmorphamine for 7 days with a 4 day expansion in 0.1μM purmorphamine and finally plated out for terminal differentiation in the presence of compound E iPSCs were maintained on Matrigel (Corning Life Sciences) with Essential 8 Medium media (Life technologies), cells were passaged using EDTA (Life technologies, 0.5mM).  RNA extraction was performed using the Promega Maxwell RSC simplyRNA kit (including DNAase treatment) and the Maxwell RSC instrument mRNA-Seq libraries were prepared using the polyA_KAPA_mRNA_Hyper_Prep kit (Roche, UK)
disease
Spinal and Bulbar Muscular Atrophy
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
sex
male
scientific_name
Homo sapiens
cell type
motor neuron
common name
human
organism part
spinal cord
developmental stage
Day 28 Motor Neuron
genotype
wild type genotype
isolate
not applicable
growth condition
DHT Treatment
cell line
iPSC derived cell line
geographic location (country and/or sea)
not collected